Moc03g23250 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc03g23250 vs. NCBI nr
Match: XP_022142447.1 (U-box domain-containing protein 35-like isoform X4 [Momordica charantia])

HSP 1 Score: 556.6 bits (1433), Expect = 1.2e-154
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180

Query: 181 ILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIES 240
           ILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIES
Sbjct: 181 ILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIES 240

Query: 241 SSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282
           SSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY
Sbjct: 241 SSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 281

BLAST of Moc03g23250 vs. NCBI nr
Match: XP_022142446.1 (U-box domain-containing protein 35-like isoform X3 [Momordica charantia])

HSP 1 Score: 552.0 bits (1421), Expect = 3.0e-153
Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = 0

Query: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180

Query: 181 ILDLISIFQIRKLILGSTKT-RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 240
           ILDLISIFQIRKLILGSTKT RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE
Sbjct: 181 ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 240

Query: 241 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282
           SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY
Sbjct: 241 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282

BLAST of Moc03g23250 vs. NCBI nr
Match: XP_022142445.1 (uncharacterized protein LOC111012574 isoform X2 [Momordica charantia])

HSP 1 Score: 551.2 bits (1419), Expect = 5.1e-153
Identity = 281/284 (98.94%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE---VKVEAVMIESDMV 180
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE   VKVEAVMIESDMV
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEFEQVKVEAVMIESDMV 180

Query: 181 AKAILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQM 240
           AKAILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQM
Sbjct: 181 AKAILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQM 240

Query: 241 IESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282
           IESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY
Sbjct: 241 IESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 284

BLAST of Moc03g23250 vs. NCBI nr
Match: XP_022142444.1 (uncharacterized protein LOC111012574 isoform X1 [Momordica charantia])

HSP 1 Score: 546.6 bits (1407), Expect = 1.3e-151
Identity = 281/285 (98.60%), Postives = 281/285 (98.60%), Query Frame = 0

Query: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE---VKVEAVMIESDMV 180
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE   VKVEAVMIESDMV
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEFEQVKVEAVMIESDMV 180

Query: 181 AKAILDLISIFQIRKLILGSTKT-RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 240
           AKAILDLISIFQIRKLILGSTKT RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ
Sbjct: 181 AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 240

Query: 241 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282
           MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY
Sbjct: 241 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 285

BLAST of Moc03g23250 vs. NCBI nr
Match: XP_038894104.1 (U-box domain-containing protein 35-like [Benincasa hispida])

HSP 1 Score: 363.2 bits (931), Expect = 2.0e-96
Identity = 198/267 (74.16%), Postives = 219/267 (82.02%), Query Frame = 0

Query: 20  GSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDCECEFEGSWFSFDFRKSNHC 79
           G ++F  DRSL  NE+  SSELFEIELGIEVRSNRKQEDDC    E SWFSFDFRKSNHC
Sbjct: 6   GGDDFLLDRSLIANEDEESSELFEIELGIEVRSNRKQEDDC----EESWFSFDFRKSNHC 65

Query: 80  VYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRFVPSPLGMLPKSQVSPRLVE 139
           VYVGI DN DSSMDAL+WTL+FAVLPST+VYL H+FPE+RFVPSPLGMLPKSQVSP+LVE
Sbjct: 66  VYVGISDNFDSSMDALQWTLDFAVLPSTIVYLFHVFPELRFVPSPLGMLPKSQVSPKLVE 125

Query: 140 RHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGSTK 199
           +  AEERAKR EFL KFVD C AA+V VE VMIESDMVAKAILDLIS+ QIRKLILGSTK
Sbjct: 126 KFKAEERAKRKEFLHKFVDKCYAAQVNVEVVMIESDMVAKAILDLISLLQIRKLILGSTK 185

Query: 200 TRKWRSKRG-ALARQVLQNAGEFCDVKIICEGKEATYQMIE--SSSSSSSNILSPNISD- 259
            RKWRS+RG  +AR VLQNA EFCDVKIICEGKE+TYQ IE  SSSSSSS+  SPN+SD 
Sbjct: 186 ARKWRSRRGKGIARHVLQNAQEFCDVKIICEGKESTYQAIESSSSSSSSSHTPSPNLSDK 245

Query: 260 NENF--DIKRMQKKYRHNSFFWCRCFG 281
           N +F    K  +K+Y  + F   RCFG
Sbjct: 246 NIHFKSHFKNTEKEYHRSGFLTWRCFG 268

BLAST of Moc03g23250 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 7.5e-06
Identity = 41/151 (27.15%), Postives = 78/151 (51.66%), Query Frame = 0

Query: 97  WTL-NFAVLPSTVVYLLHIFPEIRFVPSPLG-MLPKSQVSPRLVERHMAEERAKRTEFLR 156
           W +  FA   +    LLHI P I  VP+P+G  +P S+V   +V  +  E   +  E L+
Sbjct: 38  WAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQEILWQSEEMLK 97

Query: 157 KFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGSTKTRKWRSKRGALARQV 216
            +       +V VE ++IESD VA AI + ++   I ++++G + +R + S++  +   +
Sbjct: 98  PYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGS-SRSFFSRKADICSVI 157

Query: 217 LQNAGEFCDVKIICEGKEATYQMIESSSSSS 246
                 FC V ++ +GK +  +  +S  +++
Sbjct: 158 SALMPNFCTVYVVSKGKLSCVRPSDSDGNAT 187

BLAST of Moc03g23250 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 1.1e-04
Identity = 49/202 (24.26%), Postives = 85/202 (42.08%), Query Frame = 0

Query: 80  VYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRFVPSPLGMLPKSQVSPRLVE 139
           ++V +  ++  S   L W L         + L+H+    + +P      P   V    V 
Sbjct: 47  IFVAVDKHVAKSKSTLIWALQNT--GGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEVR 106

Query: 140 RHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGSTK 199
               +ER K    L  ++  C    V+ E + IE + +   I+ LIS   IRKL++G+  
Sbjct: 107 VFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVMGAAA 166

Query: 200 TRKWRSKRGALARQ----VLQNAGEFCDVKIICEG-----KEATYQMIESS------SSS 259
            R +  +   L  +    V + A   C +   C+G     +EAT    ES       S S
Sbjct: 167 DRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPRPSIS 226

Query: 260 SSNILSP-NISDNENFDIKRMQ 266
           +S++L   +  ++E+  I R+Q
Sbjct: 227 ASDLLQTFSTPESEHQHISRVQ 246

BLAST of Moc03g23250 vs. ExPASy TrEMBL
Match: A0A6J1CMT0 (U-box domain-containing protein 35-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111012574 PE=4 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 5.9e-155
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180

Query: 181 ILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIES 240
           ILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIES
Sbjct: 181 ILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIES 240

Query: 241 SSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282
           SSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY
Sbjct: 241 SSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 281

BLAST of Moc03g23250 vs. ExPASy TrEMBL
Match: A0A6J1CM72 (U-box domain-containing protein 35-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111012574 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.5e-153
Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = 0

Query: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180

Query: 181 ILDLISIFQIRKLILGSTKT-RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 240
           ILDLISIFQIRKLILGSTKT RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE
Sbjct: 181 ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 240

Query: 241 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282
           SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY
Sbjct: 241 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282

BLAST of Moc03g23250 vs. ExPASy TrEMBL
Match: A0A6J1CKY9 (uncharacterized protein LOC111012574 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012574 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.5e-153
Identity = 281/284 (98.94%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE---VKVEAVMIESDMV 180
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE   VKVEAVMIESDMV
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEFEQVKVEAVMIESDMV 180

Query: 181 AKAILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQM 240
           AKAILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQM
Sbjct: 181 AKAILDLISIFQIRKLILGSTKTRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQM 240

Query: 241 IESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282
           IESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY
Sbjct: 241 IESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 284

BLAST of Moc03g23250 vs. ExPASy TrEMBL
Match: A0A6J1CLJ4 (uncharacterized protein LOC111012574 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012574 PE=4 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 6.1e-152
Identity = 281/285 (98.60%), Postives = 281/285 (98.60%), Query Frame = 0

Query: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE---VKVEAVMIESDMV 180
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE   VKVEAVMIESDMV
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEFEQVKVEAVMIESDMV 180

Query: 181 AKAILDLISIFQIRKLILGSTKT-RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 240
           AKAILDLISIFQIRKLILGSTKT RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ
Sbjct: 181 AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 240

Query: 241 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282
           MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY
Sbjct: 241 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 285

BLAST of Moc03g23250 vs. ExPASy TrEMBL
Match: A0A6J1EH15 (uncharacterized protein LOC111434029 OS=Cucurbita moschata OX=3662 GN=LOC111434029 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 5.8e-94
Identity = 194/284 (68.31%), Postives = 232/284 (81.69%), Query Frame = 0

Query: 1   MMGGGIYSDDQGSTRKHYF-GSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDD 60
           ++ GG+ SD Q ST KHY+ GS++F+F RSLS NEE  SS+LFEI+LG++V+SNRK EDD
Sbjct: 203 LVEGGVRSDVQESTSKHYYLGSDDFKFHRSLSVNEEEKSSDLFEIDLGLDVQSNRKLEDD 262

Query: 61  CECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIR 120
           C    E SWFSFDFRKSNHCVYVGIGDN  SSM AL+WTL+FAVLPS++VYLLH+ PEIR
Sbjct: 263 C----EESWFSFDFRKSNHCVYVGIGDNDASSMAALQWTLDFAVLPSSIVYLLHVSPEIR 322

Query: 121 FVPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAK 180
           FVPSPLGMLP+SQV P++VE+ MAEERAKR EFL KFVDTCS+A+V VE VMIESDMVAK
Sbjct: 323 FVPSPLGMLPRSQVGPKIVEKFMAEERAKRREFLHKFVDTCSSAQVNVEVVMIESDMVAK 382

Query: 181 AILDLISIFQIRKLILG--STKTRKWRSKRGALARQVLQNAGEFCDVKIIC-EGKEATYQ 240
           AILDLIS+ QIRKLILG  STK RKWRSKR  +A+Q+L+NA EFCDVKIIC EGKE+T Q
Sbjct: 383 AILDLISLLQIRKLILGKNSTKARKWRSKREGIAKQILKNAEEFCDVKIICEEGKESTCQ 442

Query: 241 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFG 281
           +I+SSSSSSS       S + + + K M+K++ H+ F   RCFG
Sbjct: 443 VIDSSSSSSS------CSSSCSTNFKIMKKEHGHSGFVSWRCFG 476

BLAST of Moc03g23250 vs. TAIR 10
Match: AT5G47740.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 6.7e-34
Identity = 92/233 (39.48%), Postives = 134/233 (57.51%), Query Frame = 0

Query: 54  RKQEDDCECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTL-NFAVLPSTVVYLL 113
           R+ E   E + EG             VYVG+G   DSSM+AL+W + N     ST+++L+
Sbjct: 19  RRLESVRESQLEGRSSGTVSMNGEDNVYVGVGKG-DSSMEALRWAIDNLMTSSSTLLFLI 78

Query: 114 HIFPEIRFVPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMI 173
           H+FPE RF+P PLG + + + S   VE  M++ER KR   L KF+  CSA++VKVE +++
Sbjct: 79  HVFPETRFIPYPLGRITRERASQEQVESFMSQEREKRRTLLLKFLHACSASKVKVETILV 138

Query: 174 ESDMVAKAILDLISIFQIRKLILGSTKT--RKWRSKRGALARQVL--QNAGEFCDVKIIC 233
           ESD VAKA+ DLI+I  IRKL+LG  K+  RK  + +G    +++   +A + C+VK+IC
Sbjct: 139 ESDSVAKAVQDLITILNIRKLVLGIDKSNARKASTMKGNSVPELIMRSSAADVCEVKVIC 198

Query: 234 EGKEATYQ--MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCF 280
           +GKE   +  M+ESS + S               I + QKK +    F C CF
Sbjct: 199 QGKEINMEQTMMESSPAKS--------------PIVQRQKKDQPVDPFACICF 236

BLAST of Moc03g23250 vs. TAIR 10
Match: AT5G47740.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.6e-32
Identity = 92/235 (39.15%), Postives = 134/235 (57.02%), Query Frame = 0

Query: 54  RKQEDDCECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTL-NFAVLPSTVVYLL 113
           R+ E   E + EG             VYVG+G   DSSM+AL+W + N     ST+++L+
Sbjct: 19  RRLESVRESQLEGRSSGTVSMNGEDNVYVGVGKG-DSSMEALRWAIDNLMTSSSTLLFLI 78

Query: 114 HIFPEIRFVPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAA--EVKVEAV 173
           H+FPE RF+P PLG + + + S   VE  M++ER KR   L KF+  CSA+  +VKVE +
Sbjct: 79  HVFPETRFIPYPLGRITRERASQEQVESFMSQEREKRRTLLLKFLHACSASKEQVKVETI 138

Query: 174 MIESDMVAKAILDLISIFQIRKLILGSTKT--RKWRSKRGALARQVL--QNAGEFCDVKI 233
           ++ESD VAKA+ DLI+I  IRKL+LG  K+  RK  + +G    +++   +A + C+VK+
Sbjct: 139 LVESDSVAKAVQDLITILNIRKLVLGIDKSNARKASTMKGNSVPELIMRSSAADVCEVKV 198

Query: 234 ICEGKEATYQ--MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCF 280
           IC+GKE   +  M+ESS + S               I + QKK +    F C CF
Sbjct: 199 ICQGKEINMEQTMMESSPAKS--------------PIVQRQKKDQPVDPFACICF 238

BLAST of Moc03g23250 vs. TAIR 10
Match: AT5G65500.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 69.7 bits (169), Expect = 4.2e-12
Identity = 56/177 (31.64%), Postives = 90/177 (50.85%), Query Frame = 0

Query: 80  VYVGIGDNIDSSMDALKWTL-NFAVLPSTVVYLLHIFPEIR-FVPSPLGMLPKSQVSPRL 139
           VY+ +G+++      + W L  +  +P ++V LLH+    + FV +P G LP S VS   
Sbjct: 22  VYIAVGNDVQEGYKTIHWALKKWNNIPISIV-LLHLCNISQDFVYTPFGKLPASSVSEEK 81

Query: 140 VERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGS 199
           ++     E  K  + L K++  C   + ++  V  + D +   ILDLIS  +I KL++G 
Sbjct: 82  LQVLRKYEDQKIDKLLSKYITFCGKVKAELLKVEKQHDSIQVLILDLISKLRITKLVMGI 141

Query: 200 TKTR---KWRSKRGALAR-QVLQNAGEFCDVKIICEGKEATYQMIESSSSSSSNILS 251
           T  R    W+SK        V QN  EFC+  IIC GK  + +     ++++SNI S
Sbjct: 142 TFMRSSSSWKSKSAISGSFYVYQNKPEFCEFYIICGGKMVSLK--NDVNNNNSNIRS 195

BLAST of Moc03g23250 vs. TAIR 10
Match: AT5G57035.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 2.2e-08
Identity = 48/189 (25.40%), Postives = 92/189 (48.68%), Query Frame = 0

Query: 84  IGDNI--DSSMDALKWTLNFAVLPSTVVYLLHIFPEIRFVPSPLG-MLPKSQVSPRLVER 143
           +GD +   +S  AL+WT+   +     + L+H+ P +  +PSP G  +P  ++   +V  
Sbjct: 29  VGDAVGGTASRRALRWTIENFLPKIDRLVLVHVMPTVTTIPSPSGSKIPIEELDESVVSM 88

Query: 144 HMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGSTKT 203
           +  + R +  +    F   C +   KVE +++E    AKA+L  +S   +  L++GS  +
Sbjct: 89  YKRDLRKEFEQVFVPFKRICKSN--KVETLLLEHHDPAKALLKYMSDTDVECLVIGSCSS 148

Query: 204 RKWRSKRG-ALARQVLQNAGEFCDVKIICEGKEATYQMIESSSSSSSNILSPNISD--NE 263
                K+G  +   VL  A E C++ ++C+ +  T    + ++ SSS+   P  ++   E
Sbjct: 149 NFLTRKKGQEMPLTVLGEAPETCEIYVVCKDRILTKSTNQFTADSSSSFRIPEGAEAYTE 208

Query: 264 NFDIKRMQK 267
           +F   R  K
Sbjct: 209 SFSRTRSDK 215

BLAST of Moc03g23250 vs. TAIR 10
Match: AT3G61410.1 (BEST Arabidopsis thaliana protein match is: U-box domain-containing protein kinase family protein (TAIR:AT2G45910.1); Has 232 Blast hits to 229 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 13; Fungi - 0; Plants - 218; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 53.9 bits (128), Expect = 2.4e-07
Identity = 50/206 (24.27%), Postives = 92/206 (44.66%), Query Frame = 0

Query: 80  VYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRFVPSPLGMLPKSQVSPRLVE 139
           +YV +G  I ++   L W L+        + L+H  P++  +P        + V   LV 
Sbjct: 14  IYVALGREIANNKSNLAWVLDNCQGNKICIVLVHRPPQM--IPVLGTKFDAATVDEELVR 73

Query: 140 RHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILG--- 199
            +  +++AK  + L +++  C    V+ E + +E + + K I+ +IS  ++RK I+G   
Sbjct: 74  AYREKQKAKTDKILDEYLRICLRKGVQAEKLCVEMNSIEKGIVQMISENKVRKFIMGAAS 133

Query: 200 ----STKTRKWRSKRGALARQVLQNAGEFCDVKIICEG-----KEATYQMIESSS---SS 259
               STK    RSK+      V Q A   C ++  C+G     +EA    + + S   S 
Sbjct: 134 DKHFSTKMEDLRSKKAIF---VCQQASATCHIRFTCKGYLIHTREARMDEVRALSALLSD 193

Query: 260 SSNILSPNISDNENFDIKRMQKKYRH 271
              +++   S N + D    ++K  H
Sbjct: 194 FQRLVTSQSSANLDQDSGGSKRKSEH 214