Moc03g23220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g23220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Mediator of RNA polymerase II transcription subunit 11
Location	chr3: 16444884 .. 16445974 (+)
RNA-Seq Expression	Moc03g23220
Synteny	Moc03g23220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCGCTGACCCAAAATACTTCGTTACAACGACTTCAAAATGTGGAGAAGGTTGGAATGACTTTCCATACCTGAAAATTTTTGTTTCTAATTCTTTGCCTGGTCTTTTATTGAAACACTAGATTTCTTTTGACCAACTTTTGCGTTTTTCTCCTGCAGAGAATTGTGAGGGTCTTGGAGCTAGCAGGGGGAGTTATGGAAGAGCTTGCCAACCCTGCTGGTCCAAGAAAGGAATTTGTTAACAATCATTGCAGTGAGTTCATGCAATTCATCAAGGTGAGACCTCTGTTGATTTTTCAAATGTGGATTTGATAACTGAGATTGATATCTCAGCTGGTAATGCTTCAAGGTAGAATTAGATTATCCTGAACATTCGACCATGCATTAGATCGTTCACGGTAACTTGAAATTGTTATGATAAGCTGAAACCCAACCAACTGGTTCACAAAATTGAACTTTGTTTAATTGTTGCTTTTAGAGAATGTTATTATGTTAAATTTAATCCTATTAGCAGTCCAGATGAAATTTGATTACGGAAGGGGCTCTCACTTGTAACTAATTCCTGCACGGAAAAAATAGGGAATGACTGTAGAGAATAATTGAAAGATAATTGTTTTGGGGTTGGTTTGTGTATTTTTAATATTGTTAAAGATGGGGCATCATTGTCATGATTTGGTGTTTCATGAGCGACGATAATATTACCAATTTGAGTTCTAAGTAAGAGTATGTTTTGGGCTATACGTTAAAAACATTTTTCGAAAATTTCAAAAGCACTCATTTTTTCTTGTTGGAAGAAGCCATACTTGTTTCTCTGCCCCTCCTAGTAAAGTAAGTGTTTCCATTTATAACCATATAAATGCACTCCTAGATTCCACCTAAAATCTCTTTGAATTTGAAATCAGGATATCCAAGTTACGTTGCGGGATGAAATCAAAAGTGCATGTGAATACCGTCCATTCGAGAAGTGTGATTATAGTTCAAGGATAACCAATGAACTTTGCTGCAAGAAACTAGAATACATGATCTCAAAGTTGGATGGAATGAAACAAACTATAGATGAATATCAAGTGAAAGTGGAAGATGCTTGA

mRNA sequence

ATGGATCCGCTGACCCAAAATACTTCGTTACAACGACTTCAAAATGTGGAGAAGAGAATTGTGAGGGTCTTGGAGCTAGCAGGGGGAGTTATGGAAGAGCTTGCCAACCCTGCTGGTCCAAGAAAGGAATTTGTTAACAATCATTGCAGTGAGTTCATGCAATTCATCAAGGATATCCAAGTTACGTTGCGGGATGAAATCAAAAGTGCATGTGAATACCGTCCATTCGAGAAGTGTGATTATAGTTCAAGGATAACCAATGAACTTTGCTGCAAGAAACTAGAATACATGATCTCAAAGTTGGATGGAATGAAACAAACTATAGATGAATATCAAGTGAAAGTGGAAGATGCTTGA

Coding sequence (CDS)

ATGGATCCGCTGACCCAAAATACTTCGTTACAACGACTTCAAAATGTGGAGAAGAGAATTGTGAGGGTCTTGGAGCTAGCAGGGGGAGTTATGGAAGAGCTTGCCAACCCTGCTGGTCCAAGAAAGGAATTTGTTAACAATCATTGCAGTGAGTTCATGCAATTCATCAAGGATATCCAAGTTACGTTGCGGGATGAAATCAAAAGTGCATGTGAATACCGTCCATTCGAGAAGTGTGATTATAGTTCAAGGATAACCAATGAACTTTGCTGCAAGAAACTAGAATACATGATCTCAAAGTTGGATGGAATGAAACAAACTATAGATGAATATCAAGTGAAAGTGGAAGATGCTTGA

Protein sequence

MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQVTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA

Homology

[Close]

BLAST of Moc03g23220 vs. NCBI nr
Match: XP_022142440.1 (mediator of RNA polymerase II transcription subunit 11 [Momordica charantia] >XP_022142441.1 mediator of RNA polymerase II transcription subunit 11 [Momordica charantia])

HSP 1 Score: 241.9 bits (616), Expect = 2.8e-60
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ
Sbjct: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 119
           VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA
Sbjct: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 118

[Close]

BLAST of Moc03g23220 vs. NCBI nr
Match: XP_023520392.1 (mediator of RNA polymerase II transcription subunit 11 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520393.1 mediator of RNA polymerase II transcription subunit 11 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 240.7 bits (613), Expect = 6.2e-60
Identity = 117/118 (99.15%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ
Sbjct: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 119
           VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVK+EDA
Sbjct: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKMEDA 118

[Close]

BLAST of Moc03g23220 vs. NCBI nr
Match: XP_022927640.1 (mediator of RNA polymerase II transcription subunit 11 isoform X2 [Cucurbita moschata] >XP_022927641.1 mediator of RNA polymerase II transcription subunit 11 isoform X2 [Cucurbita moschata] >KAG6583673.1 Mediator of RNA polymerase II transcription subunit 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 239.6 bits (610), Expect = 1.4e-59
Identity = 116/118 (98.31%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ
Sbjct: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 119
           VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVK+ED+
Sbjct: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKMEDS 118

[Close]

BLAST of Moc03g23220 vs. NCBI nr
Match: XP_022973172.1 (mediator of RNA polymerase II transcription subunit 11 isoform X2 [Cucurbita maxima] >XP_022973173.1 mediator of RNA polymerase II transcription subunit 11 isoform X2 [Cucurbita maxima])

HSP 1 Score: 236.1 bits (601), Expect = 1.5e-58
Identity = 115/118 (97.46%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ
Sbjct: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 119
           VTLRDEIKSACEYRPFEKCDYSSRI NELCCKKLEYMI KLDGMKQTIDEYQVK+EDA
Sbjct: 61  VTLRDEIKSACEYRPFEKCDYSSRIINELCCKKLEYMILKLDGMKQTIDEYQVKMEDA 118

[Close]

BLAST of Moc03g23220 vs. NCBI nr
Match: XP_008442051.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 11 [Cucumis melo] >XP_008442052.1 PREDICTED: mediator of RNA polymerase II transcription subunit 11 [Cucumis melo] >XP_016899531.1 PREDICTED: mediator of RNA polymerase II transcription subunit 11 [Cucumis melo])

HSP 1 Score: 233.8 bits (595), Expect = 7.6e-58
Identity = 113/118 (95.76%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDP  QNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ
Sbjct: 1   MDPPAQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 119
           VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTID+YQVK+ED+
Sbjct: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDDYQVKMEDS 118

[Close]

BLAST of Moc03g23220 vs. ExPASy Swiss-Prot
Match: Q6ID77 (Mediator of RNA polymerase II transcription subunit 11 OS=Arabidopsis thaliana OX=3702 GN=MED11 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 5.3e-46
Identity = 84/111 (75.68%), Postives = 102/111 (91.89%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDP TQNTSLQRLQNVE R+V+VLELAGGVMEELA+P+GP+KEFVN+HC EFMQ +KDIQ
Sbjct: 1   MDPQTQNTSLQRLQNVENRVVKVLELAGGVMEELASPSGPKKEFVNSHCREFMQSMKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEY 112
           VTLR+EIKSACEYRPFEKCDY++RI NE+C +KLEY++++L+ +KQT D Y
Sbjct: 61  VTLREEIKSACEYRPFEKCDYNARIANEICFQKLEYVLTQLEDLKQTADRY 111

[Close]

BLAST of Moc03g23220 vs. ExPASy TrEMBL
Match: A0A6J1CKY4 (Mediator of RNA polymerase II transcription subunit 11 OS=Momordica charantia OX=3673 GN=LOC111012572 PE=3 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.4e-60
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ
Sbjct: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 119
           VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA
Sbjct: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 118

[Close]

BLAST of Moc03g23220 vs. ExPASy TrEMBL
Match: A0A6J1EPJ1 (Mediator of RNA polymerase II transcription subunit 11 OS=Cucurbita moschata OX=3662 GN=LOC111434405 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 6.7e-60
Identity = 116/118 (98.31%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ
Sbjct: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 119
           VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVK+ED+
Sbjct: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKMEDS 118

[Close]

BLAST of Moc03g23220 vs. ExPASy TrEMBL
Match: A0A6J1I6T5 (Mediator of RNA polymerase II transcription subunit 11 OS=Cucurbita maxima OX=3661 GN=LOC111471674 PE=3 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 7.4e-59
Identity = 115/118 (97.46%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ
Sbjct: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 119
           VTLRDEIKSACEYRPFEKCDYSSRI NELCCKKLEYMI KLDGMKQTIDEYQVK+EDA
Sbjct: 61  VTLRDEIKSACEYRPFEKCDYSSRIINELCCKKLEYMILKLDGMKQTIDEYQVKMEDA 118

[Close]

BLAST of Moc03g23220 vs. ExPASy TrEMBL
Match: A0A1S3B5G7 (Mediator of RNA polymerase II transcription subunit 11 OS=Cucumis melo OX=3656 GN=LOC103486032 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.7e-58
Identity = 113/118 (95.76%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDP  QNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ
Sbjct: 1   MDPPAQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEYQVKVEDA 119
           VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTID+YQVK+ED+
Sbjct: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDDYQVKMEDS 118

[Close]

BLAST of Moc03g23220 vs. ExPASy TrEMBL
Match: A0A0A0L450 (Mediator of RNA polymerase II transcription subunit 11 OS=Cucumis sativus OX=3659 GN=MED11 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.2e-54
Identity = 111/140 (79.29%), Postives = 115/140 (82.14%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIK--- 60
           MDP   NTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIK   
Sbjct: 1   MDPPAHNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKNQR 60

Query: 61  -------------------DIQVTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMI 119
                              DIQVTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYM+
Sbjct: 61  NLTKEEEWRSKMEHHDQDLDIQVTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMV 120

[Close]

BLAST of Moc03g23220 vs. TAIR 10
Match: AT3G01435.1 (Expressed protein )

HSP 1 Score: 184.9 bits (468), Expect = 3.8e-47
Identity = 84/111 (75.68%), Postives = 102/111 (91.89%), Query Frame = 0


Query: 1   MDPLTQNTSLQRLQNVEKRIVRVLELAGGVMEELANPAGPRKEFVNNHCSEFMQFIKDIQ 60
           MDP TQNTSLQRLQNVE R+V+VLELAGGVMEELA+P+GP+KEFVN+HC EFMQ +KDIQ
Sbjct: 1   MDPQTQNTSLQRLQNVENRVVKVLELAGGVMEELASPSGPKKEFVNSHCREFMQSMKDIQ 60

Query: 61  VTLRDEIKSACEYRPFEKCDYSSRITNELCCKKLEYMISKLDGMKQTIDEY 112
           VTLR+EIKSACEYRPFEKCDY++RI NE+C +KLEY++++L+ +KQT D Y
Sbjct: 61  VTLREEIKSACEYRPFEKCDYNARIANEICFQKLEYVLTQLEDLKQTADRY 111

The following BLAST results are available for this feature:

BLAST of Moc03g23220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142440.1	2.8e-60	100.00	mediator of RNA polymerase II transcription subunit 11 [Momordica charantia] >XP...	[more]
XP_023520392.1	6.2e-60	99.15	mediator of RNA polymerase II transcription subunit 11 isoform X2 [Cucurbita pep...	[more]
XP_022927640.1	1.4e-59	98.31	mediator of RNA polymerase II transcription subunit 11 isoform X2 [Cucurbita mos...	[more]
XP_022973172.1	1.5e-58	97.46	mediator of RNA polymerase II transcription subunit 11 isoform X2 [Cucurbita max...	[more]
XP_008442051.1	7.6e-58	95.76	PREDICTED: mediator of RNA polymerase II transcription subunit 11 [Cucumis melo]...	[more]

BLAST of Moc03g23220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6ID77	5.3e-46	75.68	Mediator of RNA polymerase II transcription subunit 11 OS=Arabidopsis thaliana O...	[more]

BLAST of Moc03g23220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKY4	1.4e-60	100.00	Mediator of RNA polymerase II transcription subunit 11 OS=Momordica charantia OX...	[more]
A0A6J1EPJ1	6.7e-60	98.31	Mediator of RNA polymerase II transcription subunit 11 OS=Cucurbita moschata OX=...	[more]
A0A6J1I6T5	7.4e-59	97.46	Mediator of RNA polymerase II transcription subunit 11 OS=Cucurbita maxima OX=36...	[more]
A0A1S3B5G7	3.7e-58	95.76	Mediator of RNA polymerase II transcription subunit 11 OS=Cucumis melo OX=3656 G...	[more]
A0A0A0L450	7.2e-54	79.29	Mediator of RNA polymerase II transcription subunit 11 OS=Cucumis sativus OX=365...	[more]

BLAST of Moc03g23220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01435.1	3.8e-47	75.68	Expressed protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019404	Mediator complex, subunit Med11	PFAM	PF10280	Med11	coord: 8..111 e-value: 6.0E-14 score: 52.7
IPR019404	Mediator complex, subunit Med11	PANTHER	PTHR22890	UNCHARACTERIZED	coord: 1..114
None	No IPR available	GENE3D	1.10.287.3490		coord: 7..75 e-value: 1.1E-6 score: 31.0
None	No IPR available	PANTHER	PTHR22890:SF2	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 11	coord: 1..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g23220.1	Moc03g23220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003712	transcription coregulator activity