Moc03g20230 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g20230
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr3: 13657786 .. 13658109 (+)
RNA-Seq ExpressionMoc03g20230
SyntenyMoc03g20230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGTTTCATCCTCAGAGAGAAATTCAGAATTATTTGTAACAAACAAGAATTACTCAATAGTGAATCCTGGCGATAAGATTTCAACGGTGAAATTGAATGACGAAAATTTTTTGCTGTGGAGGTTACAAGTTCTCACAGCGCTCAAAGGGCACGGTTTAGAAAAATTCATCGATCCAAATGCTGAAATTCCACCAGCCTTTGTACAATCTGGAGATGAATCATCGTCAACCTTAATTCCTAATCAGGAATTTCTCAATTGGAAAAGGCAAGACAAACTAATCACTTCATGGCTTCTTGGATCTATGACTGAAGACGTTTGA

mRNA sequence

ATGGTGGTTTCATCCTCAGAGAGAAATTCAGAATTATTTGTAACAAACAAGAATTACTCAATAGTGAATCCTGGCGATAAGATTTCAACGGTGAAATTGAATGACGAAAATTTTTTGCTGTGGAGGTTACAAGTTCTCACAGCGCTCAAAGGGCACGGTTTAGAAAAATTCATCGATCCAAATGCTGAAATTCCACCAGCCTTTGTACAATCTGGAGATGAATCATCGTCAACCTTAATTCCTAATCAGGAATTTCTCAATTGGAAAAGGCAAGACAAACTAATCACTTCATGGCTTCTTGGATCTATGACTGAAGACGTTTGA

Coding sequence (CDS)

ATGGTGGTTTCATCCTCAGAGAGAAATTCAGAATTATTTGTAACAAACAAGAATTACTCAATAGTGAATCCTGGCGATAAGATTTCAACGGTGAAATTGAATGACGAAAATTTTTTGCTGTGGAGGTTACAAGTTCTCACAGCGCTCAAAGGGCACGGTTTAGAAAAATTCATCGATCCAAATGCTGAAATTCCACCAGCCTTTGTACAATCTGGAGATGAATCATCGTCAACCTTAATTCCTAATCAGGAATTTCTCAATTGGAAAAGGCAAGACAAACTAATCACTTCATGGCTTCTTGGATCTATGACTGAAGACGTTTGA

Protein sequence

MVVSSSERNSELFVTNKNYSIVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDPNAEIPPAFVQSGDESSSTLIPNQEFLNWKRQDKLITSWLLGSMTEDV
Homology
BLAST of Moc03g20230 vs. NCBI nr
Match: XP_022159146.1 (uncharacterized protein LOC111025572 [Momordica charantia])

HSP 1 Score: 158.7 bits (400), Expect = 2.8e-35
Identity = 77/107 (71.96%), Postives = 90/107 (84.11%), Query Frame = 0

Query: 1   MVVSSSERNSELFVTNKNYSIVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDP 60
           M +SSSE +S   V N+N +IVNPG+KIST+KLNDENFLLWRLQ+ TAL+GHGL KFIDP
Sbjct: 1   MAISSSETDST--VVNQNSTIVNPGNKISTIKLNDENFLLWRLQITTALQGHGLGKFIDP 60

Query: 61  NAEIPPAFVQSGDESSSTLIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
            A+IP  FVQS DESSS   PN EF+NWKRQDKLITSWLLGSM+E++
Sbjct: 61  EAKIPSEFVQSADESSSVPSPNPEFINWKRQDKLITSWLLGSMSEEI 105

BLAST of Moc03g20230 vs. NCBI nr
Match: XP_022154487.1 (uncharacterized protein LOC111021757 [Momordica charantia])

HSP 1 Score: 108.2 bits (269), Expect = 4.4e-20
Identity = 53/109 (48.62%), Postives = 74/109 (67.89%), Query Frame = 0

Query: 1   MVVSSSERNSELFVTNKNYSIVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDP 60
           M   SS RNS+     +    +NPG K+S V+LND+N LLW+ Q+ TAL+G+GLE +ID 
Sbjct: 1   MASLSSIRNSDAARIIQASKTINPGSKVSIVRLNDDNXLLWKFQIRTALQGNGLESYIDS 60

Query: 61  NAEIPPAFVQSGDE--SSSTLIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
           N + P  FVQ+ ++  SSS+L  N  +  W +QDKLI++WLLGSM ED+
Sbjct: 61  NEDTPAQFVQTTEDESSSSSLQQNPAYFEWIKQDKLISAWLLGSMNEDI 109

BLAST of Moc03g20230 vs. NCBI nr
Match: KAA0053143.1 (keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 98.2 bits (243), Expect = 4.5e-17
Identity = 48/104 (46.15%), Postives = 68/104 (65.38%), Query Frame = 0

Query: 6   SERNSELFVTNKNYSIVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDPNAEIP 65
           S  +S L V N   SI   G+KIS VKL+D+NFLLW+ Q+LTAL+ + LE F +   E P
Sbjct: 2   SSNSSPLGVENTEVSIFGSGNKISLVKLSDDNFLLWKFQILTALEAYDLENFFESELEPP 61

Query: 66  PAFVQS--GDESSSTLIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
             ++ S     +S+T  PN E+  WKR ++LI+ WLLGSM+E++
Sbjct: 62  SKYLTSTGSSSTSATRTPNPEYKVWKRHNRLISPWLLGSMSEEI 105

BLAST of Moc03g20230 vs. NCBI nr
Match: KAA0048297.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 2.9e-16
Identity = 45/89 (50.56%), Postives = 64/89 (71.91%), Query Frame = 0

Query: 21  IVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDPNAEIPPAFVQSGDES--SST 80
           I   G+KIS VKLND+ FLLW+ Q+LTAL+ + LE F++  +E P  ++ S + S  S+T
Sbjct: 22  IFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLESESEPPSKYLISTESSSASAT 81

Query: 81  LIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
             PN  +  WKRQD+LI+SWLLGSM+E++
Sbjct: 82  GTPNPAYKVWKRQDRLISSWLLGSMSEEI 110

BLAST of Moc03g20230 vs. NCBI nr
Match: TYK10642.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 2.9e-16
Identity = 45/89 (50.56%), Postives = 64/89 (71.91%), Query Frame = 0

Query: 21  IVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDPNAEIPPAFVQSGDES--SST 80
           I   G+KIS VKLND+ FLLW+ Q+LTAL+ + LE F++  +E P  ++ S + S  S+T
Sbjct: 22  IFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLESESEPPSKYLISTESSSASAT 81

Query: 81  LIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
             PN  +  WKRQD+LI+SWLLGSM+E++
Sbjct: 82  GTPNPAYKVWKRQDRLISSWLLGSMSEEI 110

BLAST of Moc03g20230 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.4e-07
Identity = 35/99 (35.35%), Postives = 57/99 (57.58%), Query Frame = 0

Query: 9   NSELFVTNKNYSIVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDPNAEIPPAF 68
           ++E  V N N SI+N  +  +  KL   N+L+W  QV     G+ L  F+D +  +PPA 
Sbjct: 4   HAEELVLN-NTSILNV-NMSNVTKLTSTNYLMWSRQVHALFDGYELAGFLDGSTTMPPAT 63

Query: 69  VQSGDESSSTLIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
           +  G +++  +  N ++  WKRQDKLI S +LG+++  V
Sbjct: 64  I--GTDAAPRV--NPDYTRWKRQDKLIYSAVLGAISMSV 96

BLAST of Moc03g20230 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 4.4e-07
Identity = 32/97 (32.99%), Postives = 52/97 (53.61%), Query Frame = 0

Query: 11  ELFVTNKNYSIVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDPNAEIPPAFVQ 70
           E+ + N N   VN     +  KL   N+L+W  QV     G+ L  F+D +  +PPA + 
Sbjct: 7   EIVLVNTNILNVNMS---NVTKLTSTNYLMWSRQVHALFDGYELAGFLDGSTPMPPATI- 66

Query: 71  SGDESSSTLIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
            G ++   +  N ++  W+RQDKLI S +LG+++  V
Sbjct: 67  -GTDAVPRV--NPDYTRWRRQDKLIYSAILGAISMSV 96

BLAST of Moc03g20230 vs. ExPASy TrEMBL
Match: A0A6J1E314 (uncharacterized protein LOC111025572 OS=Momordica charantia OX=3673 GN=LOC111025572 PE=4 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.4e-35
Identity = 77/107 (71.96%), Postives = 90/107 (84.11%), Query Frame = 0

Query: 1   MVVSSSERNSELFVTNKNYSIVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDP 60
           M +SSSE +S   V N+N +IVNPG+KIST+KLNDENFLLWRLQ+ TAL+GHGL KFIDP
Sbjct: 1   MAISSSETDST--VVNQNSTIVNPGNKISTIKLNDENFLLWRLQITTALQGHGLGKFIDP 60

Query: 61  NAEIPPAFVQSGDESSSTLIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
            A+IP  FVQS DESSS   PN EF+NWKRQDKLITSWLLGSM+E++
Sbjct: 61  EAKIPSEFVQSADESSSVPSPNPEFINWKRQDKLITSWLLGSMSEEI 105

BLAST of Moc03g20230 vs. ExPASy TrEMBL
Match: A0A6J1DLT9 (uncharacterized protein LOC111021757 OS=Momordica charantia OX=3673 GN=LOC111021757 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.1e-20
Identity = 53/109 (48.62%), Postives = 74/109 (67.89%), Query Frame = 0

Query: 1   MVVSSSERNSELFVTNKNYSIVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDP 60
           M   SS RNS+     +    +NPG K+S V+LND+N LLW+ Q+ TAL+G+GLE +ID 
Sbjct: 1   MASLSSIRNSDAARIIQASKTINPGSKVSIVRLNDDNXLLWKFQIRTALQGNGLESYIDS 60

Query: 61  NAEIPPAFVQSGDE--SSSTLIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
           N + P  FVQ+ ++  SSS+L  N  +  W +QDKLI++WLLGSM ED+
Sbjct: 61  NEDTPAQFVQTTEDESSSSSLQQNPAYFEWIKQDKLISAWLLGSMNEDI 109

BLAST of Moc03g20230 vs. ExPASy TrEMBL
Match: A0A5A7UB21 (Keratin, type II cytoskeletal 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1486G00150 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.2e-17
Identity = 48/104 (46.15%), Postives = 68/104 (65.38%), Query Frame = 0

Query: 6   SERNSELFVTNKNYSIVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDPNAEIP 65
           S  +S L V N   SI   G+KIS VKL+D+NFLLW+ Q+LTAL+ + LE F +   E P
Sbjct: 2   SSNSSPLGVENTEVSIFGSGNKISLVKLSDDNFLLWKFQILTALEAYDLENFFESELEPP 61

Query: 66  PAFVQS--GDESSSTLIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
             ++ S     +S+T  PN E+  WKR ++LI+ WLLGSM+E++
Sbjct: 62  SKYLTSTGSSSTSATRTPNPEYKVWKRHNRLISPWLLGSMSEEI 105

BLAST of Moc03g20230 vs. ExPASy TrEMBL
Match: A0A5A7U233 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G00060 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.4e-16
Identity = 45/89 (50.56%), Postives = 64/89 (71.91%), Query Frame = 0

Query: 21  IVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDPNAEIPPAFVQSGDES--SST 80
           I   G+KIS VKLND+ FLLW+ Q+LTAL+ + LE F++  +E P  ++ S + S  S+T
Sbjct: 22  IFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLESESEPPSKYLISTESSSASAT 81

Query: 81  LIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
             PN  +  WKRQD+LI+SWLLGSM+E++
Sbjct: 82  GTPNPAYKVWKRQDRLISSWLLGSMSEEI 110

BLAST of Moc03g20230 vs. ExPASy TrEMBL
Match: A0A5D3CH97 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00040 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.4e-16
Identity = 45/89 (50.56%), Postives = 64/89 (71.91%), Query Frame = 0

Query: 21  IVNPGDKISTVKLNDENFLLWRLQVLTALKGHGLEKFIDPNAEIPPAFVQSGDES--SST 80
           I   G+KIS VKLND+ FLLW+ Q+LTAL+ + LE F++  +E P  ++ S + S  S+T
Sbjct: 22  IFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLESESEPPSKYLISTESSSASAT 81

Query: 81  LIPNQEFLNWKRQDKLITSWLLGSMTEDV 108
             PN  +  WKRQD+LI+SWLLGSM+E++
Sbjct: 82  GTPNPAYKVWKRQDRLISSWLLGSMSEEI 110

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159146.12.8e-3571.96uncharacterized protein LOC111025572 [Momordica charantia][more]
XP_022154487.14.4e-2048.62uncharacterized protein LOC111021757 [Momordica charantia][more]
KAA0053143.14.5e-1746.15keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa][more]
KAA0048297.12.9e-1650.56Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
TYK10642.12.9e-1650.56Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
Match NameE-valueIdentityDescription
Q94HW23.4e-0735.35Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT944.4e-0732.99Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1E3141.4e-3571.96uncharacterized protein LOC111025572 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DLT92.1e-2048.62uncharacterized protein LOC111021757 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A5A7UB212.2e-1746.15Keratin, type II cytoskeletal 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5A7U2331.4e-1650.56Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
A0A5D3CH971.4e-1650.56Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR37610FAMILY NOT NAMEDcoord: 22..106
NoneNo IPR availablePANTHERPTHR37610:SF39GAG-POLYPEPTIDE OF LTR COPIA-TYPE-RELATEDcoord: 22..106

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g20230.1Moc03g20230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044237 cellular metabolic process