Moc03g02700 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g02700
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionDUF4219 domain-containing protein/UBN2 domain-containing protein
Locationchr3: 2048007 .. 2048492 (-)
RNA-Seq ExpressionMoc03g02700
SyntenyMoc03g02700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCTAGGATCAAACAATCTTTCTTTACTGGCTCCACCTGTGTTTGATGGTGAAATCTATCAAACATGGGCAATCAGAATACAAGCTTACATGGAAGGTTGTGATTATTGGGAAGCAATTGAGCAAGATTATGAAATTTCTCCACTTCCTGATAATCCAACGATGCATCAGATCAAGACTCACAAGGAGAGGGTCACCAGGAAGGCAAAGGCTCGAACTTGCCTATATGCAGCTGTGTCTCCCACCATATTTAATAGAATTATGGCATTGAAGTCAGCAAAGGAGATCTGGGAGTTCCTCAAAAGTGAGTATGAAGGTGATGAGAGGATTAAAGGCATGAAGGTGTTGAACTTGGTAAGGGAATTCGAGAGAATGCAGATGAATGATTTTGAGTCCATCAAAGAGTATTCAGACAAGTTGATCGGGATTGCTAACAAGTCAAGAGCATTAGTAACTGATCTATCTGACAATAGATTGGTTTAG

mRNA sequence

ATGGAGCTAGGATCAAACAATCTTTCTTTACTGGCTCCACCTGTGTTTGATGGTGAAATCTATCAAACATGGGCAATCAGAATACAAGCTTACATGGAAGGTTGTGATTATTGGGAAGCAATTGAGCAAGATTATGAAATTTCTCCACTTCCTGATAATCCAACGATGCATCAGATCAAGACTCACAAGGAGAGGGTCACCAGGAAGGCAAAGGCTCGAACTTGCCTATATGCAGCTGTGTCTCCCACCATATTTAATAGAATTATGGCATTGAAGTCAGCAAAGGAGATCTGGGAGTTCCTCAAAAGTGAGTATGAAGGTGATGAGAGGATTAAAGGCATGAAGGTGTTGAACTTGGTAAGGGAATTCGAGAGAATGCAGATGAATGATTTTGAGTCCATCAAAGAGTATTCAGACAAGTTGATCGGGATTGCTAACAAGTCAAGAGCATTAGTAACTGATCTATCTGACAATAGATTGGTTTAG

Coding sequence (CDS)

ATGGAGCTAGGATCAAACAATCTTTCTTTACTGGCTCCACCTGTGTTTGATGGTGAAATCTATCAAACATGGGCAATCAGAATACAAGCTTACATGGAAGGTTGTGATTATTGGGAAGCAATTGAGCAAGATTATGAAATTTCTCCACTTCCTGATAATCCAACGATGCATCAGATCAAGACTCACAAGGAGAGGGTCACCAGGAAGGCAAAGGCTCGAACTTGCCTATATGCAGCTGTGTCTCCCACCATATTTAATAGAATTATGGCATTGAAGTCAGCAAAGGAGATCTGGGAGTTCCTCAAAAGTGAGTATGAAGGTGATGAGAGGATTAAAGGCATGAAGGTGTTGAACTTGGTAAGGGAATTCGAGAGAATGCAGATGAATGATTTTGAGTCCATCAAAGAGTATTCAGACAAGTTGATCGGGATTGCTAACAAGTCAAGAGCATTAGTAACTGATCTATCTGACAATAGATTGGTTTAG

Protein sequence

MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIKTHKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLVREFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV
Homology
BLAST of Moc03g02700 vs. NCBI nr
Match: XP_022157914.1 (uncharacterized protein LOC111024525 [Momordica charantia])

HSP 1 Score: 326.2 bits (835), Expect = 1.5e-85
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK
Sbjct: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
           THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV
Sbjct: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV
Sbjct: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 161

BLAST of Moc03g02700 vs. NCBI nr
Match: XP_022158688.1 (uncharacterized protein LOC111025149 [Momordica charantia])

HSP 1 Score: 301.2 bits (770), Expect = 5.3e-78
Identity = 149/161 (92.55%), Postives = 153/161 (95.03%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           MELGSNNLS LAPPVFDGE YQTWAIRIQAYMEGCDYWEAIEQDYEI+PLPDNPTMHQIK
Sbjct: 1   MELGSNNLSSLAPPVFDGENYQTWAIRIQAYMEGCDYWEAIEQDYEIAPLPDNPTMHQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
           THKERVTRKAKAR CLYAAVSP IFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV
Sbjct: 61  THKERVTRKAKARACLYAAVSPAIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFER+QM D ESIKEYSDKLIGIANK+RAL  DLS+NRLV
Sbjct: 121 REFERIQMKDSESIKEYSDKLIGIANKARALGIDLSNNRLV 161

BLAST of Moc03g02700 vs. NCBI nr
Match: XP_022152365.1 (uncharacterized protein LOC111020109 [Momordica charantia])

HSP 1 Score: 300.8 bits (769), Expect = 6.9e-78
Identity = 148/161 (91.93%), Postives = 153/161 (95.03%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           MELGSNNLS LAPPVFDGE YQ WAIRIQAYMEGC+YWEAIEQDYEI+PLPDNPTMHQIK
Sbjct: 1   MELGSNNLSSLAPPVFDGENYQAWAIRIQAYMEGCNYWEAIEQDYEIAPLPDNPTMHQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
           THKERVTRKAKAR CLYAAVSP IFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV
Sbjct: 61  THKERVTRKAKARACLYAAVSPAIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFERMQM D +SIKEYSDKLIGIANK+RAL TD+SDNRLV
Sbjct: 121 REFERMQMKDSDSIKEYSDKLIGIANKARALGTDVSDNRLV 161

BLAST of Moc03g02700 vs. NCBI nr
Match: XP_022151329.1 (uncharacterized protein LOC111019291 [Momordica charantia])

HSP 1 Score: 297.0 bits (759), Expect = 1.0e-76
Identity = 147/161 (91.30%), Postives = 149/161 (92.55%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           ME GSNNLS LAPPVFDGE YQ W IRIQAYMEGCDYWEAIEQDYEI+PLPDNPTMHQIK
Sbjct: 1   MEFGSNNLSSLAPPVFDGENYQAWTIRIQAYMEGCDYWEAIEQDYEIAPLPDNPTMHQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
           THK RVTRKAKAR CLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDER KGM VLNLV
Sbjct: 61  THKVRVTRKAKARACLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERTKGMTVLNLV 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFERMQM D ESIKEYSDKLIGIANK+RAL TDLSDNRLV
Sbjct: 121 REFERMQMKDSESIKEYSDKLIGIANKARALGTDLSDNRLV 161

BLAST of Moc03g02700 vs. NCBI nr
Match: XP_038889190.1 (uncharacterized protein LOC120079069 [Benincasa hispida])

HSP 1 Score: 278.9 bits (712), Expect = 2.8e-71
Identity = 137/161 (85.09%), Postives = 145/161 (90.06%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           ME GSN+LS L P VFDGE YQ WAIR+QAYME CDYWE IEQDYEI+PLPDNPT++QIK
Sbjct: 1   MESGSNSLSSLVPLVFDGENYQAWAIRMQAYMEDCDYWEVIEQDYEIAPLPDNPTIYQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
           THKERVTRK KAR CLY AVSP IFNRIMALKS KEIWEFLKSEYEGDERIKGMKVLNLV
Sbjct: 61  THKERVTRKEKARVCLYVAVSPAIFNRIMALKSKKEIWEFLKSEYEGDERIKGMKVLNLV 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFERMQM D++SIKEYSDKLIGIANK+RAL  DLSDNRLV
Sbjct: 121 REFERMQMKDYKSIKEYSDKLIGIANKARALGIDLSDNRLV 161

BLAST of Moc03g02700 vs. ExPASy TrEMBL
Match: A0A6J1DXX3 (uncharacterized protein LOC111024525 OS=Momordica charantia OX=3673 GN=LOC111024525 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 7.5e-86
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK
Sbjct: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
           THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV
Sbjct: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV
Sbjct: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 161

BLAST of Moc03g02700 vs. ExPASy TrEMBL
Match: A0A6J1DWT9 (uncharacterized protein LOC111025149 OS=Momordica charantia OX=3673 GN=LOC111025149 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.6e-78
Identity = 149/161 (92.55%), Postives = 153/161 (95.03%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           MELGSNNLS LAPPVFDGE YQTWAIRIQAYMEGCDYWEAIEQDYEI+PLPDNPTMHQIK
Sbjct: 1   MELGSNNLSSLAPPVFDGENYQTWAIRIQAYMEGCDYWEAIEQDYEIAPLPDNPTMHQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
           THKERVTRKAKAR CLYAAVSP IFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV
Sbjct: 61  THKERVTRKAKARACLYAAVSPAIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFER+QM D ESIKEYSDKLIGIANK+RAL  DLS+NRLV
Sbjct: 121 REFERIQMKDSESIKEYSDKLIGIANKARALGIDLSNNRLV 161

BLAST of Moc03g02700 vs. ExPASy TrEMBL
Match: A0A6J1DDR0 (uncharacterized protein LOC111020109 OS=Momordica charantia OX=3673 GN=LOC111020109 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.4e-78
Identity = 148/161 (91.93%), Postives = 153/161 (95.03%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           MELGSNNLS LAPPVFDGE YQ WAIRIQAYMEGC+YWEAIEQDYEI+PLPDNPTMHQIK
Sbjct: 1   MELGSNNLSSLAPPVFDGENYQAWAIRIQAYMEGCNYWEAIEQDYEIAPLPDNPTMHQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
           THKERVTRKAKAR CLYAAVSP IFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV
Sbjct: 61  THKERVTRKAKARACLYAAVSPAIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFERMQM D +SIKEYSDKLIGIANK+RAL TD+SDNRLV
Sbjct: 121 REFERMQMKDSDSIKEYSDKLIGIANKARALGTDVSDNRLV 161

BLAST of Moc03g02700 vs. ExPASy TrEMBL
Match: A0A6J1DCR7 (uncharacterized protein LOC111019291 OS=Momordica charantia OX=3673 GN=LOC111019291 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.8e-77
Identity = 147/161 (91.30%), Postives = 149/161 (92.55%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           ME GSNNLS LAPPVFDGE YQ W IRIQAYMEGCDYWEAIEQDYEI+PLPDNPTMHQIK
Sbjct: 1   MEFGSNNLSSLAPPVFDGENYQAWTIRIQAYMEGCDYWEAIEQDYEIAPLPDNPTMHQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
           THK RVTRKAKAR CLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDER KGM VLNLV
Sbjct: 61  THKVRVTRKAKARACLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERTKGMTVLNLV 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFERMQM D ESIKEYSDKLIGIANK+RAL TDLSDNRLV
Sbjct: 121 REFERMQMKDSESIKEYSDKLIGIANKARALGTDLSDNRLV 161

BLAST of Moc03g02700 vs. ExPASy TrEMBL
Match: A0A1U8PA84 (uncharacterized protein LOC107955882 OS=Gossypium hirsutum OX=3635 GN=LOC107955882 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.0e-63
Identity = 118/161 (73.29%), Postives = 139/161 (86.34%), Query Frame = 0

Query: 1   MELGSNNLSLLAPPVFDGEIYQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTMHQIK 60
           ME GS+NLS+LAPPVFDGE YQ WA+R+QAYMEGCDYWE IE+DYE++PLP NPTM+QIK
Sbjct: 1   MESGSSNLSILAPPVFDGENYQAWAVRMQAYMEGCDYWEVIEEDYEVTPLPKNPTMNQIK 60

Query: 61  THKERVTRKAKARTCLYAAVSPTIFNRIMALKSAKEIWEFLKSEYEGDERIKGMKVLNLV 120
            H ER TRK KAR+CLYA+VSP IFNRIMA  S KEIW++LK +Y+GDERIK MKVLNL+
Sbjct: 61  MHNERTTRKTKARSCLYASVSPAIFNRIMAFGSTKEIWDYLKVDYQGDERIKSMKVLNLI 120

Query: 121 REFERMQMNDFESIKEYSDKLIGIANKSRALVTDLSDNRLV 162
           REFER+Q+ + +SIKEYSDKLI IANK R L  DLSD++LV
Sbjct: 121 REFERLQIKESKSIKEYSDKLIDIANKVRVLGADLSDSKLV 161

BLAST of Moc03g02700 vs. TAIR 10
Match: AT3G21000.1 (Gag-Pol-related retrotransposon family protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.3e-05
Identity = 32/145 (22.07%), Postives = 69/145 (47.59%), Query Frame = 0

Query: 21  YQTWAIRIQAYMEGCDYWEAIEQDYEISPLPDNPTM------HQIKTHKERVTRKAKART 80
           Y+ WA   ++ +     W+ +       P   NP +       ++   ++ V + AKA  
Sbjct: 17  YEIWAPITKSTLIEQGLWDVVVNGVPQDP-SKNPELAATIQPEELSKWRDFVVKDAKALQ 76

Query: 81  CLYAAVSPTIFNRIMALKSAKEIWEFLK--SEYEGDERIKGMKVLNLVREFERMQMNDFE 140
            L ++++ ++F + ++  SAK++W+ L+  +E     R++ + +  L ++ E ++M D E
Sbjct: 77  ILQSSLTDSVFRKTLSASSAKDVWDLLRKGNEQATIRRLEQVTIRRLEKQLEDLKMVDKE 136

Query: 141 SIKEYSDKLIGIANKSRALVTDLSD 158
           S   Y DK + I  +      + SD
Sbjct: 137 SGSSYLDKALEILERLGRAKLEKSD 160

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022157914.11.5e-85100.00uncharacterized protein LOC111024525 [Momordica charantia][more]
XP_022158688.15.3e-7892.55uncharacterized protein LOC111025149 [Momordica charantia][more]
XP_022152365.16.9e-7891.93uncharacterized protein LOC111020109 [Momordica charantia][more]
XP_022151329.11.0e-7691.30uncharacterized protein LOC111019291 [Momordica charantia][more]
XP_038889190.12.8e-7185.09uncharacterized protein LOC120079069 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DXX37.5e-86100.00uncharacterized protein LOC111024525 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1DWT92.6e-7892.55uncharacterized protein LOC111025149 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DDR03.4e-7891.93uncharacterized protein LOC111020109 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1DCR74.8e-7791.30uncharacterized protein LOC111019291 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A1U8PA841.0e-6373.29uncharacterized protein LOC107955882 OS=Gossypium hirsutum OX=3635 GN=LOC1079558... [more]
Match NameE-valueIdentityDescription
AT3G21000.11.3e-0522.07Gag-Pol-related retrotransposon family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 67..160
e-value: 8.5E-14
score: 51.5
NoneNo IPR availablePANTHERPTHR35317OS04G0629600 PROTEINcoord: 2..161
NoneNo IPR availablePANTHERPTHR35317:SF11SUBFAMILY NOT NAMEDcoord: 2..161

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g02700.1Moc03g02700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding