Moc02g12930 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g12930
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionGTP-binding protein At2g22870
Locationchr2: 9429406 .. 9432928 (-)
RNA-Seq ExpressionMoc02g12930
SyntenyMoc02g12930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATACTGAAGAGCCGCCTGCAGAATCTCCCTCTCGCAGCCGTCTTCTCCGCCGCTCTCTCTCCAAAAACCTCCCCCGGCCCCCTCTCTCTCTCTTTCAGAACCCCACTTTCGCATTCCGCCGCCCCCCACCCTCCGCCGGAGGGCCTAAAACGTTCCGCTTCCAACGCCGCCAAGCCCTCGATCTCCGACAGCGCTCGGTTTGCTCGGACGGTTCTGTTTATTCCCCCTGGAGTCGAGCCCGATGAGGTCTCCGAAGAAATGATCCTGCCGGGCTCCAACATTGTGCTCGGCCCTTACGCCGGCGACTCTCGGGTCAAGGAGGTGGAATTCGTGAAGAGCAGCGCTCGAGCCAGGGATTGCCCTAAAGATGATAAACCGGAATTTGCGATTCTTGGTCGCTCCAATGTCGGCAAATCTTCCCTTATCAATGCCCTTGTTCGCAAAAAGGAAGTGGCGCTTACGTCTAAGAAACCAGGTCTTCTGTATTCATTGTCTACTTTTGAATTTGGTTTTGAATTTTGGGGATTCGCCATGTGCGATTAGATAGGTTTGGTGGCTACTGGGTTTTTGACGGTTATGAAATTTCATCTGATTGCAGGGAAGACGCAGCTGATTAATCACTTCTTGGTGAATAAAAGCTGGTACATAGTAGACTTACCGGGCTATGGGTAATTCCTGTTCTCTTTCTTGGAAGTTATTTTTATTCATTATGAACTTGAACTTTCAATTTTGACTTGTCTAAAAGTCAGTTTAATGCTTATATGCTATATTTACTGTATTATTTAAGGATTAGGTTAGAAATCATGATGCTTACCTAATGACAAAAACTGTGTTAGGAAGCCAAAGAACCTGTTACACATCAAATTGGGGGTCTAGAGACTTATTAGAAACTTTTTAAAATACAAAGACTAAATAAATACGACTCTGCATAACACGTTGGTTTAGCATTTACATGATTCAACTCCTCCGTGTACATCTTTATAATGATTGTTAAGTTTCTGTACAAATTTTTGCAATTACCTTTTTAATTTTGGCATAGTGGAGCTGCCTTTTTGTTGAAATTTTGTATTGGAGAAGCATTGGAGCTTCCTTATGTTTATATGATTTGAATAACACAGCGGAAGGAATGACTGTTTTCTTGCAACCAACTGTTAGTGTATCGGTCTTAAGTTTCTAATTGTGTAGTCCAATATCTGCTCTCTGTGGTTCATCTCTCAGTAATACACTATGGCTCTTCTAAAAGTTTTGTACTTGGTATTTTAAGTTGATAGCAACTTAGAATTTGCCTGCCCTTTCTAACTTCTATTGCTTTTTGACTGAACGGGTAGGCCATACTAGTCTGTATCTTGTCTATAATTTCTTGATTAGTAGGAAAATTGGGCGATCGGCAAACTAGTACACAATCCTGTTTAAGAGATTAATTACAAACAACTCGAGGCATTTCATAAGAAAAAACATAATTGATAATATAAAGTTAAAAAAGGGCATAAATGCATTTTCAAAATTATGCTATGGGACAAATATTAGTTTTCTTATGGTAGTGGTGGTATAATGCTATATTTGTGCCCCCTGCATTATGCAAGATAGATGGAGAGTAATGTATTTAAAAGGTGTTCTTGCCTCTTGGTTTTATTTTGAAGTCAGCTCTCTGGCCTATGATTTTTTGACCATAAATATAATTTCTTTTCGGACTCTATTGGACTCTCACTATGATGTTTTGTTTCCATCATCTACTTGTGGGGTGAGTTTTTTATTGGTTTTGGGACCATTGTGAAGAATTCTTTGCCATCAAGAAAAACTCAGCAATGAATCTTTCAGATATCTTAGTCGGTGTTTTAAAATGATTTTGAGAATATAAATGTGCTTTCTAATATGTAGTCAAACATGAAGATTTATTTTTAACCAGCACAAAGTGGTTCTTGAAATACCTTAGAAATATTGCCTAACATTTTGAAGTTGAGTCCACAAGCCTTGATATAGAGAAATTTCTCATGATAGTGTGAGATATCTCCTCACTTGATTATTTTCTGATAATGAAGAAAAAGCCTCAATGGCGATACAAGCCACCCCACTAATTCGAGAGGGTGGGTAACCTCTCCTCCTACCCCAGTACAAGGCACAAAAGCATAGACTGATCTCTCCCTACCCTACGAGGCCCTTAACTAACCCCTCGCACCAACACGCTCCCCTTCCCCTGGCCCACATTCTCTCTCTAGGCCTTTATCCCCTCCTTGTTCATTTCCCAATCTACCCCTTCTGGAATAAGTCTTCAATATAGGTGGTCTAACAGATAGTTTATTAAATTTTAAATTCTTATTCAGTTTGTTAGAAAATTGTTGCTGCTGCTGTATTGAAATGGAGATGAATCAAAATGAACTACACTTCTTAGACAAATATCAATTCTTGACTCGAGACAAATCATTACCATTTGAAGTTTGTTTGCTTTTTTTTTTTTTTTTTCCTTTTCTTTTTTGCAATCAAGCTAAGAATGAATCTTCGTCCCCATAGAATCAAAATCTCCTAACATCTGCATATTTGAGCCTTAGGTTGGAATCATTTTGTTCCTCTATGTGAAATATTTGATCCTCTTTACTTGTTTTCCAGCTACGTCATCTTGTATGGTCTCCTTGATATGCAACCATTGGATTTTGAATGTGTCTGTGTTCTTTCGAGTCCCAAATATTTTTTCTCCACTCTTTGAGAATCAATCTTTCATATTGCCTCTTTGTGATTGAAAATGAATCACATTAAGACGTCTCAAGGAATAATCAATCATCATTCTCAAGTATTTCAATAGAATTGGAAGATTTGGACAATGTGTGGAAGAGTATTGAAATTCTAGTATAATGTTGGATCCAAAATGTATAACAGTATAAGAGTTTGTTATTGTATAGTATTTAAAGGGGATCTTTTGTTGACATTTTATCGATATTGATATAGTTTTTTAAACTATTTTTGAATAAAACATAATGCAATCTCTATTCTACATCTTGTAAAATATCATTGTTTAATACTACAAATTCGATTTAGTTTTGCCAAAGCCTCAGAAGCTGCTCGAATGGATTGGTCCTCGTTCACGAAAGGTTATTTTATAAACCGTGATACTCTGGTAGCTGTGCTGCTTCTTATTGATGCAAGTGTTCCACCTCAAAAAATCGACTTAGATTGTGCCAATTGGCTTGGTCGCAACAATGTATGGCATCTAACTTTTAAGTTCATTGCTTTGTATGTGTGCAAAATGTTCTAGTTGGATTATAGTCCTTGTCTGATGCATTTGCTGAACGTTTTCTGTTGTGTCATGTTTTTTCAGATACCGATGACATTTGTCTTCACAAAGTGCGACAAAATGAAGGTGAGCAAAGGAAAAAGACCCGACGAGAACATCAGGGACTTTCAACAACTTATCAGACAAAATTACAAGGTACATCCTTCTTGGATCATGACGAGTAGCGTCACGGGGTTAGGTCGGGATGAATTGCTTCTACACATGTCCCAACTGAGGAATTTCTGGGATCAATAA

mRNA sequence

ATGATACTGAAGAGCCGCCTGCAGAATCTCCCTCTCGCAGCCGTCTTCTCCGCCGCTCTCTCTCCAAAAACCTCCCCCGGCCCCCTCTCTCTCTCTTTCAGAACCCCACTTTCGCATTCCGCCGCCCCCCACCCTCCGCCGGAGGGCCTAAAACGTTCCGCTTCCAACGCCGCCAAGCCCTCGATCTCCGACAGCGCTCGGTTTGCTCGGACGGTTCTGTTTATTCCCCCTGGAGTCGAGCCCGATGAGGTCTCCGAAGAAATGATCCTGCCGGGCTCCAACATTGTGCTCGGCCCTTACGCCGGCGACTCTCGGGTCAAGGAGGTGGAATTCGTGAAGAGCAGCGCTCGAGCCAGGGATTGCCCTAAAGATGATAAACCGGAATTTGCGATTCTTGGTCGCTCCAATGTCGGCAAATCTTCCCTTATCAATGCCCTTGTTCGCAAAAAGGAAGTGGCGCTTACGTCTAAGAAACCAGGGAAGACGCAGCTGATTAATCACTTCTTGGTGAATAAAAGCTGGTACATAGTAGACTTACCGGGCTATGGTTTTGCCAAAGCCTCAGAAGCTGCTCGAATGGATTGGTCCTCGTTCACGAAAGGTTATTTTATAAACCGTGATACTCTGGTAGCTGTGCTGCTTCTTATTGATGCAAGTGTTCCACCTCAAAAAATCGACTTAGATTGTGCCAATTGGCTTGGTCGCAACAATATACCGATGACATTTGTCTTCACAAAGTGCGACAAAATGAAGGTGAGCAAAGGAAAAAGACCCGACGAGAACATCAGGGACTTTCAACAACTTATCAGACAAAATTACAAGGTACATCCTTCTTGGATCATGACGAGTAGCGTCACGGGGTTAGGTCGGGATGAATTGCTTCTACACATGTCCCAACTGAGGAATTTCTGGGATCAATAA

Coding sequence (CDS)

ATGATACTGAAGAGCCGCCTGCAGAATCTCCCTCTCGCAGCCGTCTTCTCCGCCGCTCTCTCTCCAAAAACCTCCCCCGGCCCCCTCTCTCTCTCTTTCAGAACCCCACTTTCGCATTCCGCCGCCCCCCACCCTCCGCCGGAGGGCCTAAAACGTTCCGCTTCCAACGCCGCCAAGCCCTCGATCTCCGACAGCGCTCGGTTTGCTCGGACGGTTCTGTTTATTCCCCCTGGAGTCGAGCCCGATGAGGTCTCCGAAGAAATGATCCTGCCGGGCTCCAACATTGTGCTCGGCCCTTACGCCGGCGACTCTCGGGTCAAGGAGGTGGAATTCGTGAAGAGCAGCGCTCGAGCCAGGGATTGCCCTAAAGATGATAAACCGGAATTTGCGATTCTTGGTCGCTCCAATGTCGGCAAATCTTCCCTTATCAATGCCCTTGTTCGCAAAAAGGAAGTGGCGCTTACGTCTAAGAAACCAGGGAAGACGCAGCTGATTAATCACTTCTTGGTGAATAAAAGCTGGTACATAGTAGACTTACCGGGCTATGGTTTTGCCAAAGCCTCAGAAGCTGCTCGAATGGATTGGTCCTCGTTCACGAAAGGTTATTTTATAAACCGTGATACTCTGGTAGCTGTGCTGCTTCTTATTGATGCAAGTGTTCCACCTCAAAAAATCGACTTAGATTGTGCCAATTGGCTTGGTCGCAACAATATACCGATGACATTTGTCTTCACAAAGTGCGACAAAATGAAGGTGAGCAAAGGAAAAAGACCCGACGAGAACATCAGGGACTTTCAACAACTTATCAGACAAAATTACAAGGTACATCCTTCTTGGATCATGACGAGTAGCGTCACGGGGTTAGGTCGGGATGAATTGCTTCTACACATGTCCCAACTGAGGAATTTCTGGGATCAATAA

Protein sequence

MILKSRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAAKPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNFWDQ
Homology
BLAST of Moc02g12930 vs. NCBI nr
Match: XP_022149441.1 (GTP-binding protein At2g22870 [Momordica charantia])

HSP 1 Score: 614.4 bits (1583), Expect = 5.4e-172
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 0

Query: 1   MILKSRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAAKP 60
           MILKSRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAAKP
Sbjct: 1   MILKSRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAAKP 60

Query: 61  SISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD
Sbjct: 61  SISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120

Query: 121 CPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP 180
           CPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP
Sbjct: 121 CPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP 180

Query: 181 GYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPM 240
           GYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPM
Sbjct: 181 GYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPM 240

Query: 241 TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL 300
           TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL
Sbjct: 241 TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL 300

Query: 301 RNFWDQ 307
           RNFWDQ
Sbjct: 301 RNFWDQ 306

BLAST of Moc02g12930 vs. NCBI nr
Match: XP_022986232.1 (GTP-binding protein At2g22870 [Cucurbita maxima])

HSP 1 Score: 533.1 bits (1372), Expect = 1.6e-147
Identity = 270/308 (87.66%), Postives = 284/308 (92.21%), Query Frame = 0

Query: 1   MILK--SRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAA 60
           MILK  SRL  LPLA++FS  LSPK+SP  L +S  +PLSHS AP+  P  L   ++  A
Sbjct: 1   MILKTLSRLHTLPLASLFSPTLSPKSSPHFLPIS--SPLSHSTAPNRSPSLLASVSNTTA 60

Query: 61  KPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120
           K SISDSARFARTVLF+PPGV+ DEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA
Sbjct: 61  KTSISDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120

Query: 121 RDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180
           RDCPKD+KPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD
Sbjct: 121 RDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180

Query: 181 LPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240
           LPGYGFAKASE ARMDWSSFTKGYFINRDTLVAVLLL+DASVPPQKIDLDCANWLGRNNI
Sbjct: 181 LPGYGFAKASETARMDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNI 240

Query: 241 PMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300
           P+TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS
Sbjct: 241 PLTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300

Query: 301 QLRNFWDQ 307
           QLRN+WDQ
Sbjct: 301 QLRNYWDQ 306

BLAST of Moc02g12930 vs. NCBI nr
Match: XP_022927961.1 (GTP-binding protein At2g22870 [Cucurbita moschata])

HSP 1 Score: 530.4 bits (1365), Expect = 1.0e-146
Identity = 269/308 (87.34%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 1   MILK--SRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAA 60
           MILK  SRL  LPLA++FS  LSPK+SP  L +S  +PLSHS AP+  P  L   ++  A
Sbjct: 1   MILKTLSRLHTLPLASLFSPTLSPKSSPHFLPVS--SPLSHSTAPNRSPSLLASVSNTTA 60

Query: 61  KPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120
           K SISDSARFARTVLF+PPGV+ DEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA
Sbjct: 61  KTSISDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120

Query: 121 RDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180
           RDCPKD+KPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD
Sbjct: 121 RDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180

Query: 181 LPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240
           LPGYGFAKASE AR DWSSFTKGYFINRDTLVAVLLL+DASVPPQKIDLDCANWLGRNNI
Sbjct: 181 LPGYGFAKASETARTDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNI 240

Query: 241 PMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300
           P+TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS
Sbjct: 241 PLTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300

Query: 301 QLRNFWDQ 307
           QLRN+WDQ
Sbjct: 301 QLRNYWDQ 306

BLAST of Moc02g12930 vs. NCBI nr
Match: KAG6571274.1 (GTP-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 530.0 bits (1364), Expect = 1.3e-146
Identity = 268/308 (87.01%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 1   MILK--SRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAA 60
           MILK  +RL  LPLA++FS  LSPK+SP  L +S  +PLSHS AP+  P  L   ++  A
Sbjct: 1   MILKTLTRLHTLPLASLFSPTLSPKSSPHFLPIS--SPLSHSTAPNRSPSLLASVSNTTA 60

Query: 61  KPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120
           K SISDSARFARTVLF+PPGV+ D VSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA
Sbjct: 61  KASISDSARFARTVLFVPPGVDADHVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120

Query: 121 RDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180
           RDCPKD+KPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD
Sbjct: 121 RDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180

Query: 181 LPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240
           LPGYGFAKASE ARMDWSSFTKGYFINRDTLVAVLLL+DASVPPQKIDLDCANWLGRNNI
Sbjct: 181 LPGYGFAKASETARMDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNI 240

Query: 241 PMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300
           P+TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS
Sbjct: 241 PLTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300

Query: 301 QLRNFWDQ 307
           QLRN+WDQ
Sbjct: 301 QLRNYWDQ 306

BLAST of Moc02g12930 vs. NCBI nr
Match: KAG7011050.1 (GTP-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 530.0 bits (1364), Expect = 1.3e-146
Identity = 268/308 (87.01%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 1   MILK--SRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAA 60
           MILK  +RL  LPLA++FS  LSPK+SP  L +S  +PLSHS AP+  P  L   ++  A
Sbjct: 1   MILKTLTRLHTLPLASLFSPTLSPKSSPHFLPIS--SPLSHSTAPNRSPSLLASVSNTTA 60

Query: 61  KPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120
           K SISDSARFARTVLF+PPGV+ D VSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA
Sbjct: 61  KTSISDSARFARTVLFVPPGVDADHVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120

Query: 121 RDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180
           RDCPKD+KPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD
Sbjct: 121 RDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180

Query: 181 LPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240
           LPGYGFAKASE ARMDWSSFTKGYFINRDTLVAVLLL+DASVPPQKIDLDCANWLGRNNI
Sbjct: 181 LPGYGFAKASETARMDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNI 240

Query: 241 PMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300
           P+TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS
Sbjct: 241 PLTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300

Query: 301 QLRNFWDQ 307
           QLRN+WDQ
Sbjct: 301 QLRNYWDQ 306

BLAST of Moc02g12930 vs. ExPASy Swiss-Prot
Match: O81004 (GTP-binding protein At2g22870 OS=Arabidopsis thaliana OX=3702 GN=EMB2001 PE=2 SV=2)

HSP 1 Score: 444.9 bits (1143), Expect = 7.4e-124
Identity = 219/306 (71.57%), Postives = 255/306 (83.33%), Query Frame = 0

Query: 1   MILKSRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAAKP 60
           M+L  R ++L    +    L PK+       SFR P   S    P       + +     
Sbjct: 1   MVLLLRYRSL---TINLTPLIPKSQKFHTLQSFRNPNFISI---PKISASTNNPTTTTNR 60

Query: 61  SISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SISD+ +FA++VLFIPPGVE +E++++M+LPGSNIV+GP+AG S++KEVEFVKSSARARD
Sbjct: 61  SISDATKFAKSVLFIPPGVEIEELTDDMVLPGSNIVIGPFAGHSQIKEVEFVKSSARARD 120

Query: 121 CPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP 180
           CPKDD+PE AILGRSNVGKSSLIN LVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP
Sbjct: 121 CPKDDRPEIAILGRSNVGKSSLINCLVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP 180

Query: 181 GYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPM 240
           GYGFAK S+AA+ DWS+FTKGYF+NRD+LV VLLLIDASVPPQKIDLDCANWLGRNN+PM
Sbjct: 181 GYGFAKVSDAAKTDWSAFTKGYFLNRDSLVCVLLLIDASVPPQKIDLDCANWLGRNNVPM 240

Query: 241 TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL 300
           TFVFTKCDKMK +KGKRPDENI+ FQQ+IR+N+KVHP WI+TSSV+GLGRDELLLHMSQL
Sbjct: 241 TFVFTKCDKMKATKGKRPDENIKAFQQIIRENFKVHPPWILTSSVSGLGRDELLLHMSQL 300

Query: 301 RNFWDQ 307
           RN+WDQ
Sbjct: 301 RNYWDQ 300

BLAST of Moc02g12930 vs. ExPASy Swiss-Prot
Match: A0M537 (Probable GTP-binding protein EngB OS=Gramella forsetii (strain KT0803) OX=411154 GN=engB PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.1e-46
Identity = 85/198 (42.93%), Postives = 132/198 (66.67%), Query Frame = 0

Query: 105 RVKEVEFVKSSARARDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQL 164
           ++K  EFV S+++   CP    PE+A +GRSNVGKSSLIN L  +K +A TS KPGKTQL
Sbjct: 2   KIKTAEFVISNSKVDHCPNSTLPEYAFIGRSNVGKSSLINMLTGRKSLAKTSAKPGKTQL 61

Query: 165 INHFLVNKSWYIVDLPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQK 224
           INHFL+N +W++VDLPGYG+A+ S++ +  +  F   YF  R+ ++   +LID+   PQ 
Sbjct: 62  INHFLINNNWHLVDLPGYGYAQVSKSTKKVFQKFITAYFKKREQMICAFVLIDSRHKPQP 121

Query: 225 IDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSS 284
           ID++   WLG +NIP   +FTK DK+   K K  D+NI +++  + +++   P + +TS+
Sbjct: 122 IDMEFMQWLGEHNIPFCIIFTKADKL---KPKILDKNIENYKNEMLESWVEMPEYFITSA 181

Query: 285 VTGLGRDELLLHMSQLRN 303
            + LG+D++L ++  + N
Sbjct: 182 TSKLGQDDILDYIEGINN 196

BLAST of Moc02g12930 vs. ExPASy Swiss-Prot
Match: A5FIZ1 (Probable GTP-binding protein EngB OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=engB PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.4e-45
Identity = 87/196 (44.39%), Postives = 125/196 (63.78%), Query Frame = 0

Query: 105 RVKEVEFVKSSARARDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQL 164
           ++   EF+ S++ A  CPKD  PE+A +GRSNVGKSSLIN +   K +A TS KPGKTQL
Sbjct: 2   KINTAEFIVSNSDAAKCPKDFLPEYAFIGRSNVGKSSLINMITNHKNLAKTSGKPGKTQL 61

Query: 165 INHFLVNKSWYIVDLPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQK 224
           INHF +N +W++VDLPGYG+AK S+  +  +  F   YF NR+ LV   +LID     QK
Sbjct: 62  INHFKINNNWFLVDLPGYGYAKVSKKTKSIFQQFITDYFENREQLVCAFVLIDIRHEAQK 121

Query: 225 IDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSS 284
           ID++  +++G + IP   +FTK D  K+SKGK         +Q+   N+   P + +TSS
Sbjct: 122 IDIEFMSYMGESEIPFCIIFTKAD--KISKGKIDSHIAAYRKQMYANNWAEMPQYFVTSS 181

Query: 285 VTGLGRDELLLHMSQL 301
              +G+DELL ++ ++
Sbjct: 182 TESIGKDELLTYIDEV 195

BLAST of Moc02g12930 vs. ExPASy Swiss-Prot
Match: A6LBY4 (Probable GTP-binding protein EngB OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152) OX=435591 GN=engB PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.6e-44
Identity = 87/197 (44.16%), Postives = 126/197 (63.96%), Query Frame = 0

Query: 106 VKEVEFVKSSARARDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLI 165
           +K  EFV S+   R CP+ +KPE+A +GRSNVGKSSLIN L  KK +A+TS+ PGKT LI
Sbjct: 3   IKSAEFVISNTDVRKCPEGNKPEYAFIGRSNVGKSSLINMLTNKKGLAMTSQTPGKTLLI 62

Query: 166 NHFLVNKSWYIVDLPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKI 225
           NHFL+N  WY+VDLPGYGFA+  +  R       + Y ++R+ L  + +L+D     QKI
Sbjct: 63  NHFLINNEWYLVDLPGYGFAQRGKENREQLKRIIENYILDREQLTNLFVLLDCRHEAQKI 122

Query: 226 DLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSV 285
           DL+   WLG + +P + +FTK D  K+SKG R  EN++ +   + + ++  P   ++SS 
Sbjct: 123 DLEFMEWLGESGVPFSIIFTKID--KISKG-RLKENLKVYTDKLLETWEELPPIFLSSSE 182

Query: 286 TGLGRDELLLHMSQLRN 303
              GRDE+L ++  + N
Sbjct: 183 KKEGRDEILDYIESINN 196

BLAST of Moc02g12930 vs. ExPASy Swiss-Prot
Match: Q5LDL2 (Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=engB PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 9.9e-44
Identity = 85/195 (43.59%), Postives = 123/195 (63.08%), Query Frame = 0

Query: 106 VKEVEFVKSSARARDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLI 165
           +   EFV S+   + CP    PE+A +GRSNVGKSSLIN L  +K +A+TS  PGKT LI
Sbjct: 3   ITNAEFVISNTDVKKCPAGTFPEYAFIGRSNVGKSSLINMLTGRKGLAMTSATPGKTMLI 62

Query: 166 NHFLVNKSWYIVDLPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKI 225
           NHFL+N SWY+VDLPGYG+A+  +  +    +  + Y + R+ +  + +LID+ + PQKI
Sbjct: 63  NHFLINNSWYLVDLPGYGYARRGQKGQKQIRTIIEDYILEREQMTNLFVLIDSRLEPQKI 122

Query: 226 DLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSV 285
           DL+   WLG N IP   +FTK DK+   KG R   NI  + + +R+ ++  P + +TSS 
Sbjct: 123 DLEFMEWLGENGIPFAIIFTKADKL---KGGRLKINISAYLRELRKQWEELPPYFITSSE 182

Query: 286 TGLGRDELLLHMSQL 301
             LGR E+L ++  +
Sbjct: 183 ERLGRTEVLNYIESI 194

BLAST of Moc02g12930 vs. ExPASy TrEMBL
Match: A0A6J1D8D5 (GTP-binding protein At2g22870 OS=Momordica charantia OX=3673 GN=LOC111017869 PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 2.6e-172
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 0

Query: 1   MILKSRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAAKP 60
           MILKSRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAAKP
Sbjct: 1   MILKSRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAAKP 60

Query: 61  SISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD
Sbjct: 61  SISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120

Query: 121 CPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP 180
           CPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP
Sbjct: 121 CPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP 180

Query: 181 GYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPM 240
           GYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPM
Sbjct: 181 GYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPM 240

Query: 241 TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL 300
           TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL
Sbjct: 241 TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL 300

Query: 301 RNFWDQ 307
           RNFWDQ
Sbjct: 301 RNFWDQ 306

BLAST of Moc02g12930 vs. ExPASy TrEMBL
Match: A0A6J1J701 (GTP-binding protein At2g22870 OS=Cucurbita maxima OX=3661 GN=LOC111484051 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 7.6e-148
Identity = 270/308 (87.66%), Postives = 284/308 (92.21%), Query Frame = 0

Query: 1   MILK--SRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAA 60
           MILK  SRL  LPLA++FS  LSPK+SP  L +S  +PLSHS AP+  P  L   ++  A
Sbjct: 1   MILKTLSRLHTLPLASLFSPTLSPKSSPHFLPIS--SPLSHSTAPNRSPSLLASVSNTTA 60

Query: 61  KPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120
           K SISDSARFARTVLF+PPGV+ DEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA
Sbjct: 61  KTSISDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120

Query: 121 RDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180
           RDCPKD+KPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD
Sbjct: 121 RDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180

Query: 181 LPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240
           LPGYGFAKASE ARMDWSSFTKGYFINRDTLVAVLLL+DASVPPQKIDLDCANWLGRNNI
Sbjct: 181 LPGYGFAKASETARMDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNI 240

Query: 241 PMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300
           P+TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS
Sbjct: 241 PLTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300

Query: 301 QLRNFWDQ 307
           QLRN+WDQ
Sbjct: 301 QLRNYWDQ 306

BLAST of Moc02g12930 vs. ExPASy TrEMBL
Match: A0A6J1EIY5 (GTP-binding protein At2g22870 OS=Cucurbita moschata OX=3662 GN=LOC111434809 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 4.9e-147
Identity = 269/308 (87.34%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 1   MILK--SRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAA 60
           MILK  SRL  LPLA++FS  LSPK+SP  L +S  +PLSHS AP+  P  L   ++  A
Sbjct: 1   MILKTLSRLHTLPLASLFSPTLSPKSSPHFLPVS--SPLSHSTAPNRSPSLLASVSNTTA 60

Query: 61  KPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120
           K SISDSARFARTVLF+PPGV+ DEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA
Sbjct: 61  KTSISDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120

Query: 121 RDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180
           RDCPKD+KPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD
Sbjct: 121 RDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180

Query: 181 LPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240
           LPGYGFAKASE AR DWSSFTKGYFINRDTLVAVLLL+DASVPPQKIDLDCANWLGRNNI
Sbjct: 181 LPGYGFAKASETARTDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNI 240

Query: 241 PMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300
           P+TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS
Sbjct: 241 PLTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300

Query: 301 QLRNFWDQ 307
           QLRN+WDQ
Sbjct: 301 QLRNYWDQ 306

BLAST of Moc02g12930 vs. ExPASy TrEMBL
Match: A0A5D3BI84 (GTP-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00870 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 9.0e-141
Identity = 259/308 (84.09%), Postives = 272/308 (88.31%), Query Frame = 0

Query: 1   MILK--SRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAA 60
           M+LK  SRL   PLA++FS  LSPK SP  LS S R               L  S++  A
Sbjct: 1   MLLKNFSRLHTFPLASLFSPTLSPKPSPHSLSFSSR---------------LVSSSNATA 60

Query: 61  KPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120
           K SISDSARFARTVLFIPPGV+ D++S+E ILPGSNIVLGPYAGDSRVKEVEFVKSS RA
Sbjct: 61  KSSISDSARFARTVLFIPPGVDADDISDEKILPGSNIVLGPYAGDSRVKEVEFVKSSGRA 120

Query: 121 RDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180
           RDCPKD+KPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD
Sbjct: 121 RDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180

Query: 181 LPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240
           LPGYGFAKASE ARMDWSSFTKGYF+NRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI
Sbjct: 181 LPGYGFAKASETARMDWSSFTKGYFMNRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240

Query: 241 PMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300
           PMTFVFTKCDKMKVSKGKRPDENIR+FQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS
Sbjct: 241 PMTFVFTKCDKMKVSKGKRPDENIREFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 293

Query: 301 QLRNFWDQ 307
           QLRN+WDQ
Sbjct: 301 QLRNYWDQ 293

BLAST of Moc02g12930 vs. ExPASy TrEMBL
Match: A0A1S3C6C3 (GTP-binding protein At2g22870 OS=Cucumis melo OX=3656 GN=LOC103497360 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 9.0e-141
Identity = 259/308 (84.09%), Postives = 272/308 (88.31%), Query Frame = 0

Query: 1   MILK--SRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAA 60
           M+LK  SRL   PLA++FS  LSPK SP  LS S R               L  S++  A
Sbjct: 1   MLLKNFSRLHTFPLASLFSPTLSPKPSPHSLSFSSR---------------LVSSSNATA 60

Query: 61  KPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120
           K SISDSARFARTVLFIPPGV+ D++S+E ILPGSNIVLGPYAGDSRVKEVEFVKSS RA
Sbjct: 61  KSSISDSARFARTVLFIPPGVDADDISDEKILPGSNIVLGPYAGDSRVKEVEFVKSSGRA 120

Query: 121 RDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180
           RDCPKD+KPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD
Sbjct: 121 RDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVD 180

Query: 181 LPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240
           LPGYGFAKASE ARMDWSSFTKGYF+NRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI
Sbjct: 181 LPGYGFAKASETARMDWSSFTKGYFMNRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNI 240

Query: 241 PMTFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 300
           PMTFVFTKCDKMKVSKGKRPDENIR+FQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS
Sbjct: 241 PMTFVFTKCDKMKVSKGKRPDENIREFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMS 293

Query: 301 QLRNFWDQ 307
           QLRN+WDQ
Sbjct: 301 QLRNYWDQ 293

BLAST of Moc02g12930 vs. TAIR 10
Match: AT2G22870.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 444.9 bits (1143), Expect = 5.3e-125
Identity = 219/306 (71.57%), Postives = 255/306 (83.33%), Query Frame = 0

Query: 1   MILKSRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAAKP 60
           M+L  R ++L    +    L PK+       SFR P   S    P       + +     
Sbjct: 1   MVLLLRYRSL---TINLTPLIPKSQKFHTLQSFRNPNFISI---PKISASTNNPTTTTNR 60

Query: 61  SISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SISD+ +FA++VLFIPPGVE +E++++M+LPGSNIV+GP+AG S++KEVEFVKSSARARD
Sbjct: 61  SISDATKFAKSVLFIPPGVEIEELTDDMVLPGSNIVIGPFAGHSQIKEVEFVKSSARARD 120

Query: 121 CPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP 180
           CPKDD+PE AILGRSNVGKSSLIN LVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP
Sbjct: 121 CPKDDRPEIAILGRSNVGKSSLINCLVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLP 180

Query: 181 GYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPM 240
           GYGFAK S+AA+ DWS+FTKGYF+NRD+LV VLLLIDASVPPQKIDLDCANWLGRNN+PM
Sbjct: 181 GYGFAKVSDAAKTDWSAFTKGYFLNRDSLVCVLLLIDASVPPQKIDLDCANWLGRNNVPM 240

Query: 241 TFVFTKCDKMKVSKGKRPDENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL 300
           TFVFTKCDKMK +KGKRPDENI+ FQQ+IR+N+KVHP WI+TSSV+GLGRDELLLHMSQL
Sbjct: 241 TFVFTKCDKMKATKGKRPDENIKAFQQIIRENFKVHPPWILTSSVSGLGRDELLLHMSQL 300

Query: 301 RNFWDQ 307
           RN+WDQ
Sbjct: 301 RNYWDQ 300

BLAST of Moc02g12930 vs. TAIR 10
Match: AT5G11480.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.5e-82
Identity = 148/259 (57.14%), Postives = 187/259 (72.20%), Query Frame = 0

Query: 61  SISDS-------ARFARTVLFIPPGVEPDEVSEE------MILPGSNIVLGPYAGDSRVK 120
           S+SDS          A   LFIPP  E D   E+       IL GSNIVL  YA D++V 
Sbjct: 59  SVSDSTDLDDAPVEIALDKLFIPP--ETDISGEDSASLTTRILKGSNIVLSKYARDAQVV 118

Query: 121 EVEFVKSSARARDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINH 180
           + ++VKSS +  DCP D  PEFA++GRSNVGKSSL+N+LVR+K +ALTSKKPGKTQ INH
Sbjct: 119 QADYVKSSVKTEDCPADGLPEFALVGRSNVGKSSLLNSLVRRKRLALTSKKPGKTQCINH 178

Query: 181 FLVNKSWYIVDLPGYGFAKASEAARMDWSSFTKGYFINRDTLVAVLLLIDASVPPQKIDL 240
           F +N  WY+VDLPGYG+A A    + DW+ FTK YF+NR TLV+V LL+DAS+P + IDL
Sbjct: 179 FRINDKWYLVDLPGYGYASAPHELKQDWNKFTKDYFLNRSTLVSVFLLVDASIPVKPIDL 238

Query: 241 DCANWLGRNNIPMTFVFTKCDKMKVSK--GKRPDENIRDFQQLIRQNYKVHPSWIMTSSV 300
           + A+WLG+N +PMT +FTKCDK K  K  GK+P+ NI++FQ LI+  ++  P WIMTSSV
Sbjct: 239 EYASWLGQNQVPMTLIFTKCDKRKKKKNGGKKPEANIKEFQDLIQGFFETTPPWIMTSSV 298

Query: 301 TGLGRDELLLHMSQLRNFW 305
           T  GRDE+LLHM+QLRN+W
Sbjct: 299 TNQGRDEILLHMAQLRNYW 315

BLAST of Moc02g12930 vs. TAIR 10
Match: AT5G58370.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 2.1e-25
Identity = 74/224 (33.04%), Postives = 107/224 (47.77%), Query Frame = 0

Query: 87  EMILPGSNIVLGPYAGDSRVKE------VEFVKSSARARDCPKDDKPEFAILGRSNVGKS 146
           E+  P  NI         R++E      +EF  ++  +   P  D PE A  GRSNVGKS
Sbjct: 243 ELPAPLDNIPQSTSTERERIEESVFRNKLEFFAAANVSSSFPPPDVPEIAFAGRSNVGKS 302

Query: 147 SLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLPGYGFAKASEAARMDWSSFTK 206
           SL+NAL R+  V  TS KPG TQ IN F +     +VDLPGYGFA A +  +  W    K
Sbjct: 303 SLLNALTRQWGVVRTSDKPGLTQTINFFGLGPKVRLVDLPGYGFAFAKDEVKEAWEDLVK 362

Query: 207 GYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKM-KVSKGKRPD 266
            Y   R +L  V LL+D     +  D +  N + R+N     V TK D +  +   +R  
Sbjct: 363 EYVSTRTSLKRVCLLVDTKWGMKPRDQELINLMERSNTKYQIVLTKTDVVFPIDVARR-- 422

Query: 267 ENIRDFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNF 304
                 ++ ++ N  +    +M SS +G G   L   ++++  F
Sbjct: 423 --AMQIEEKLKANRSIVQPLMMVSSRSGAGIGSLRTALAKIARF 462

BLAST of Moc02g12930 vs. TAIR 10
Match: AT5G58370.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 6.8e-24
Identity = 65/168 (38.69%), Postives = 84/168 (50.00%), Query Frame = 0

Query: 87  EMILPGSNIVLGPYAGDSRVKE------VEFVKSSARARDCPKDDKPEFAILGRSNVGKS 146
           E+  P  NI         R++E      +EF  ++  +   P  D PE A  GRSNVGKS
Sbjct: 243 ELPAPLDNIPQSTSTERERIEESVFRNKLEFFAAANVSSSFPPPDVPEIAFAGRSNVGKS 302

Query: 147 SLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLPGYGFAKASEAARMDWSSFTK 206
           SL+NAL R+  V  TS KPG TQ IN F +     +VDLPGYGFA A +  +  W    K
Sbjct: 303 SLLNALTRQWGVVRTSDKPGLTQTINFFGLGPKVRLVDLPGYGFAFAKDEVKEAWEDLVK 362

Query: 207 GYFINRDTLVAVLLLIDASVPPQKIDLDCANWLGRNNIPMTFVFTKCD 249
            Y   R +L  V LL+D     +  D +  N + R+N     V TK D
Sbjct: 363 EYVSTRTSLKRVCLLVDTKWGMKPRDQELINLMERSNTKYQIVLTKTD 410

BLAST of Moc02g12930 vs. TAIR 10
Match: AT1G08410.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 9.3e-05
Identity = 25/66 (37.88%), Postives = 35/66 (53.03%), Query Frame = 0

Query: 125 DKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLPGYGF 184
           D+     +G  NVGKSS INALV +K   +TS  PGKT+     +++    + D PG  F
Sbjct: 307 DQAVVGFVGYPNVGKSSTINALVGQKRTGVTS-TPGKTKHFQTLIISDELMLCDCPGLVF 366

Query: 185 AKASEA 191
              S +
Sbjct: 367 PSFSSS 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149441.15.4e-172100.00GTP-binding protein At2g22870 [Momordica charantia][more]
XP_022986232.11.6e-14787.66GTP-binding protein At2g22870 [Cucurbita maxima][more]
XP_022927961.11.0e-14687.34GTP-binding protein At2g22870 [Cucurbita moschata][more]
KAG6571274.11.3e-14687.01GTP-binding protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7011050.11.3e-14687.01GTP-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
O810047.4e-12471.57GTP-binding protein At2g22870 OS=Arabidopsis thaliana OX=3702 GN=EMB2001 PE=2 SV... [more]
A0M5372.1e-4642.93Probable GTP-binding protein EngB OS=Gramella forsetii (strain KT0803) OX=411154... [more]
A5FIZ12.4e-4544.39Probable GTP-binding protein EngB OS=Flavobacterium johnsoniae (strain ATCC 1706... [more]
A6LBY42.6e-4444.16Probable GTP-binding protein EngB OS=Parabacteroides distasonis (strain ATCC 850... [more]
Q5LDL29.9e-4443.59Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain ATCC 25285 / D... [more]
Match NameE-valueIdentityDescription
A0A6J1D8D52.6e-172100.00GTP-binding protein At2g22870 OS=Momordica charantia OX=3673 GN=LOC111017869 PE=... [more]
A0A6J1J7017.6e-14887.66GTP-binding protein At2g22870 OS=Cucurbita maxima OX=3661 GN=LOC111484051 PE=3 S... [more]
A0A6J1EIY54.9e-14787.34GTP-binding protein At2g22870 OS=Cucurbita moschata OX=3662 GN=LOC111434809 PE=3... [more]
A0A5D3BI849.0e-14184.09GTP-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G... [more]
A0A1S3C6C39.0e-14184.09GTP-binding protein At2g22870 OS=Cucumis melo OX=3656 GN=LOC103497360 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G22870.15.3e-12571.57P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G11480.12.5e-8257.14P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G58370.22.1e-2533.04P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G58370.16.8e-2438.69P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G08410.19.3e-0537.88P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019987GTP-binding protein, ribosome biogenesis, YsxCTIGRFAMTIGR03598TIGR03598coord: 110..296
e-value: 1.5E-58
score: 195.5
IPR019987GTP-binding protein, ribosome biogenesis, YsxCHAMAPMF_00321GTPase_EngBcoord: 114..301
score: 23.894402
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 101..302
e-value: 2.0E-55
score: 189.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 118..302
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 129..246
e-value: 1.6E-22
score: 79.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..57
NoneNo IPR availablePANTHERPTHR11649:SF75PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 53..306
NoneNo IPR availablePANTHERPTHR11649MSS1/TRME-RELATED GTP-BINDING PROTEINcoord: 53..306
IPR030393EngB-type guanine nucleotide-binding (G) domainPROSITEPS51706G_ENGBcoord: 125..303
score: 35.617107
IPR030393EngB-type guanine nucleotide-binding (G) domainCDDcd01876YihA_EngBcoord: 128..300
e-value: 1.14492E-71
score: 216.993

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g12930.1Moc02g12930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005525 GTP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding