Moc02g12540 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g12540
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	chr2: 9111885 .. 9113295 (+)
RNA-Seq Expression	Moc02g12540
Synteny	Moc02g12540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAACAAGCTTCTCTCTTCACTTCTCTTCTTCACTTTCCTTCTGGGTCTTACTAGTAAGCATCTCCCTTCTCCCCCCCTCCCATTTCTCACTTCAGTTCTTAACCCCTTAATGTCAGTTTCTTCCGTTAATATTGATAATCACTAGTCGGGTCAGTGTTAGAATATGAATGATTGATTATATGTTTATACGTACCTTTTAATTTTTGGGTCTAAATGGCAGATGCAGGTCAAGAATCCATTCAAGTTGTGAAGCTGGATGAGAAGACTTCAGGGGTTTTTCCAGCACTGTCTGGTTCTGAACTCCCTGTTGCTGTTTCTGTAACTGATGCTGAACTTGCAGAGGTCTCAAGCAGTGTTTTAATGGCGGAAAGTTGGCTTAGAACCTTCGTTCTGGCTCACTACCCTTCCACTAAAATCACCACCATTGTTGTGGGAAACAGCTCTCTGTGCAGCAACAACTCCAATTTGGTCTTACTTTCTCTCAAGAACTTGTTTCACTCTCTTACGAGATGGGGTTTGGAGACTCAAATCAAAGTCTCAACTCTCTTCTCAATCGATTGCTTCCACTCAGATCCTCAAGAGGAGGATTCCGCTGAGCACATGCTGAAACTTGTGTTCGATTTCATCAAAGCTACAAACTCCACGTTGACCCTCAAACTACCTGAAAATGCAATTTCTCAAGATGAAATGGACAGCTTCATCTCGTCCAAATTGGAAAAAATTGGGTTTCTAGAGCTCGAGAAATTGAATCTAATAACAAGCGTCTCAAATCAGAGAAACCCAATTGGCAGGAAGCTCAATTTCATTGAATTCGATGACCCTCTCTTTACAGCAGAGCCGCCATTGCCATCGGACCTAGCCAGACCCCCAAATCCACCGGAGTCCCAAATCGCATCTCCGCCACAGATGGGGTTCGCCGCCGCCCCGGAGCTGCCGCCGTTCGTGGTCCCGGCGAGTCCGCCGATGGGGTTCGCGCTGCCGCCGTGCAATCCGGACCAAAATGCTGGTGCGCCATTTCCACAAACAGGTGGCATGCAGAAGCAGTGGTGCGTGGCGAAGCCGAGCGTGCCGGTGGAGATTCTGCAGCAGGCGATGGACTACGCCTGCGGCGACGGCGGCGCCGACTGTAAGGAGATATCGGCGGACGGGAGCTGCTTCCATCCTGATACTCTGGTGGCGCACGCCTCTTATGCGTTCAATAACTACTGGCAGAAGAATAAGAGAAGTGGAGGAACCTGTAGCTTTGGGGGCACCGCCATGATCATCAGTTCGGACCCAAGTGAGAATCTCTGTGAATTTCTTCTTTTGTTACAAGTTTTTGTTGATTGGGAAATTTTGATGAATTGGGTTTCTTGTTTTGTTCTTGTGTTGCAGGTTTTCATCACTGCCGCTTTGTTCTCAGCTGA

mRNA sequence

ATGAAGAACAAGCTTCTCTCTTCACTTCTCTTCTTCACTTTCCTTCTGGGTCTTACTAATGCAGGTCAAGAATCCATTCAAGTTGTGAAGCTGGATGAGAAGACTTCAGGGGTTTTTCCAGCACTGTCTGGTTCTGAACTCCCTGTTGCTGTTTCTGTAACTGATGCTGAACTTGCAGAGGTCTCAAGCAGTGTTTTAATGGCGGAAAGTTGGCTTAGAACCTTCGTTCTGGCTCACTACCCTTCCACTAAAATCACCACCATTGTTGTGGGAAACAGCTCTCTGTGCAGCAACAACTCCAATTTGGTCTTACTTTCTCTCAAGAACTTGTTTCACTCTCTTACGAGATGGGGTTTGGAGACTCAAATCAAAGTCTCAACTCTCTTCTCAATCGATTGCTTCCACTCAGATCCTCAAGAGGAGGATTCCGCTGAGCACATGCTGAAACTTGTGTTCGATTTCATCAAAGCTACAAACTCCACGTTGACCCTCAAACTACCTGAAAATGCAATTTCTCAAGATGAAATGGACAGCTTCATCTCGTCCAAATTGGAAAAAATTGGGTTTCTAGAGCTCGAGAAATTGAATCTAATAACAAGCGTCTCAAATCAGAGAAACCCAATTGGCAGGAAGCTCAATTTCATTGAATTCGATGACCCTCTCTTTACAGCAGAGCCGCCATTGCCATCGGACCTAGCCAGACCCCCAAATCCACCGGAGTCCCAAATCGCATCTCCGCCACAGATGGGGTTCGCCGCCGCCCCGGAGCTGCCGCCGTTCGTGGTCCCGGCGAGTCCGCCGATGGGGTTCGCGCTGCCGCCGTGCAATCCGGACCAAAATGCTGGTGCGCCATTTCCACAAACAGGTGGCATGCAGAAGCAGTGGTGCGTGGCGAAGCCGAGCGTGCCGGTGGAGATTCTGCAGCAGGCGATGGACTACGCCTGCGGCGACGGCGGCGCCGACTGTAAGGAGATATCGGCGGACGGGAGCTGCTTCCATCCTGATACTCTGGTGGCGCACGCCTCTTATGCGTTCAATAACTACTGGCAGAAGAATAAGAGAAGTGGAGGAACCTGTAGCTTTGGGGGCACCGCCATGATCATCAGTTCGGACCCAAGTTTTCATCACTGCCGCTTTGTTCTCAGCTGA

Coding sequence (CDS)

ATGAAGAACAAGCTTCTCTCTTCACTTCTCTTCTTCACTTTCCTTCTGGGTCTTACTAATGCAGGTCAAGAATCCATTCAAGTTGTGAAGCTGGATGAGAAGACTTCAGGGGTTTTTCCAGCACTGTCTGGTTCTGAACTCCCTGTTGCTGTTTCTGTAACTGATGCTGAACTTGCAGAGGTCTCAAGCAGTGTTTTAATGGCGGAAAGTTGGCTTAGAACCTTCGTTCTGGCTCACTACCCTTCCACTAAAATCACCACCATTGTTGTGGGAAACAGCTCTCTGTGCAGCAACAACTCCAATTTGGTCTTACTTTCTCTCAAGAACTTGTTTCACTCTCTTACGAGATGGGGTTTGGAGACTCAAATCAAAGTCTCAACTCTCTTCTCAATCGATTGCTTCCACTCAGATCCTCAAGAGGAGGATTCCGCTGAGCACATGCTGAAACTTGTGTTCGATTTCATCAAAGCTACAAACTCCACGTTGACCCTCAAACTACCTGAAAATGCAATTTCTCAAGATGAAATGGACAGCTTCATCTCGTCCAAATTGGAAAAAATTGGGTTTCTAGAGCTCGAGAAATTGAATCTAATAACAAGCGTCTCAAATCAGAGAAACCCAATTGGCAGGAAGCTCAATTTCATTGAATTCGATGACCCTCTCTTTACAGCAGAGCCGCCATTGCCATCGGACCTAGCCAGACCCCCAAATCCACCGGAGTCCCAAATCGCATCTCCGCCACAGATGGGGTTCGCCGCCGCCCCGGAGCTGCCGCCGTTCGTGGTCCCGGCGAGTCCGCCGATGGGGTTCGCGCTGCCGCCGTGCAATCCGGACCAAAATGCTGGTGCGCCATTTCCACAAACAGGTGGCATGCAGAAGCAGTGGTGCGTGGCGAAGCCGAGCGTGCCGGTGGAGATTCTGCAGCAGGCGATGGACTACGCCTGCGGCGACGGCGGCGCCGACTGTAAGGAGATATCGGCGGACGGGAGCTGCTTCCATCCTGATACTCTGGTGGCGCACGCCTCTTATGCGTTCAATAACTACTGGCAGAAGAATAAGAGAAGTGGAGGAACCTGTAGCTTTGGGGGCACCGCCATGATCATCAGTTCGGACCCAAGTTTTCATCACTGCCGCTTTGTTCTCAGCTGA

Protein sequence

MKNKLLSSLLFFTFLLGLTNAGQESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSNLVLLSLKNLFHSLTRWGLETQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFISSKLEKIGFLELEKLNLITSVSNQRNPIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPESQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKPSVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCSFGGTAMIISSDPSFHHCRFVLS

Homology

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BLAST of Moc02g12540 vs. NCBI nr
Match: XP_022149437.1 (glucan endo-1,3-beta-glucosidase 13 [Momordica charantia])

HSP 1 Score: 755.0 bits (1948), Expect = 3.2e-214
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0


Query: 1   MKNKLLSSLLFFTFLLGLTNAGQESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAE 60
           MKNKLLSSLLFFTFLLGLTNAGQESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAE
Sbjct: 1   MKNKLLSSLLFFTFLLGLTNAGQESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAE 60

Query: 61  VSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSNLVLLSLKNLFHSLTRWGLE 120
           VSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSNLVLLSLKNLFHSLTRWGLE
Sbjct: 61  VSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSNLVLLSLKNLFHSLTRWGLE 120

Query: 121 TQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFI 180
           TQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFI
Sbjct: 121 TQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFI 180

Query: 181 SSKLEKIGFLELEKLNLITSVSNQRNPIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPE 240
           SSKLEKIGFLELEKLNLITSVSNQRNPIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPE
Sbjct: 181 SSKLEKIGFLELEKLNLITSVSNQRNPIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPE 240

Query: 241 SQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKP 300
           SQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKP
Sbjct: 241 SQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKP 300

Query: 301 SVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCS 360
           SVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCS
Sbjct: 301 SVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCS 360

Query: 361 FGGTAMIISSDPSFHHCRFVLS 383
           FGGTAMIISSDPSFHHCRFVLS
Sbjct: 361 FGGTAMIISSDPSFHHCRFVLS 382

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BLAST of Moc02g12540 vs. NCBI nr
Match: XP_038900662.1 (glucan endo-1,3-beta-glucosidase 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 589.0 bits (1517), Expect = 3.0e-164
Identity = 307/388 (79.12%), Postives = 337/388 (86.86%), Query Frame = 0


Query: 5   LLSSLLFFTFLLGLTNAGQESIQVVKLDE-KTSGVFPALSGSELPVAVSVTDAELAEVSS 64
           L     FFTFLLGLT AGQESIQ VKLDE  +SGVFPA+SGS+LPVAVSVTDAEL EVSS
Sbjct: 7   LFPFFFFFTFLLGLTTAGQESIQFVKLDEIISSGVFPAISGSDLPVAVSVTDAELPEVSS 66

Query: 65  SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCS------NNSNLVLLSLKNLFHSLTRW 124
           SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCS      N    VLLSLKNLF+SLTRW
Sbjct: 67  SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSTKNQDTNTLQFVLLSLKNLFYSLTRW 126

Query: 125 GLETQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMD 184
           GLE QIKVSTLF  DCFHS   +E+S ++M+KLV +FI++TNSTLTLKL EN IS  E +
Sbjct: 127 GLENQIKVSTLFPKDCFHS---QEESIQNMVKLVIEFIQSTNSTLTLKLTENVISLHETE 186

Query: 185 SFISSKLEKIGFLELEKLNLITSVSNQRNPIGRKL-NFIEFD--DPLFTAEPPLPSDLAR 244
           +FIS+ + K GFL+L K+NLITSV+NQRNPI RKL +F+E +  +P FT+EPPLPSDLA 
Sbjct: 187 NFISTHMNKFGFLKLHKVNLITSVTNQRNPISRKLSSFMELNEYEPFFTSEPPLPSDLAT 246

Query: 245 PPNPPESQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQ 304
           PP PPESQIASPP   FAAAPE PPFVVPASPPMGF+LPPCNPDQNAGAPFPQTG +QKQ
Sbjct: 247 PPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFSLPPCNPDQNAGAPFPQTGSVQKQ 306

Query: 305 WCVAKPSVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKR 364
           WCVAKPSVP EILQQAMDYACGDGGADC+EISA+GSCFHPDTLVAHASYAFN+YWQKNKR
Sbjct: 307 WCVAKPSVPTEILQQAMDYACGDGGADCREISAEGSCFHPDTLVAHASYAFNSYWQKNKR 366

Query: 365 SGGTCSFGGTAMIISSDPSFHHCRFVLS 383
           +GGTCSFGGTAMIISSDPSFHHCRFVLS
Sbjct: 367 NGGTCSFGGTAMIISSDPSFHHCRFVLS 391

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BLAST of Moc02g12540 vs. NCBI nr
Match: XP_008457775.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 13 [Cucumis melo])

HSP 1 Score: 583.6 bits (1503), Expect = 1.3e-162
Identity = 305/389 (78.41%), Postives = 336/389 (86.38%), Query Frame = 0


Query: 5   LLSSLLFFTFLLGLTNAGQESIQVVKLDE-KTSGVFPALSGSELPVAVSVTDAELAEVSS 64
           LL    FF FLLGLT AGQESIQ VKL+E  +SGVFPA+S SELPVAVS+TD EL EVSS
Sbjct: 14  LLFFFFFFFFLLGLTTAGQESIQFVKLEEIVSSGVFPAMSASELPVAVSLTDVELPEVSS 73

Query: 65  SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSNL-------VLLSLKNLFHSLTR 124
           SVLMAESWLRTFVLAHYPSTKI+TIVVGNSSLCSN +NL       VLLS+KNLF+SLTR
Sbjct: 74  SVLMAESWLRTFVLAHYPSTKISTIVVGNSSLCSNKNNLDTNNLHIVLLSMKNLFYSLTR 133

Query: 125 WGLETQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEM 184
           WGLE QIKVSTLF  DCFHS   +E+S ++M+KLV +FI++TNSTL+LKLPEN IS  E 
Sbjct: 134 WGLENQIKVSTLFPKDCFHS---QEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHET 193

Query: 185 DSFISSKLEKIGFLELEKLNLITSVSNQRNPIGRKL-NFIEFD--DPLFTAEPPLPSDLA 244
           +SFIS+   K GFL+L K+NL+TSVS QRNPI RKL +F+E    DP FT+EPPLPSDLA
Sbjct: 194 ESFISTHTNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYDPFFTSEPPLPSDLA 253

Query: 245 RPPNPPESQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQK 304
            PP PPESQIASPP  GFAAAPE PPFVVPASPPMGF LPPCNPDQNAGAPFPQTGG+QK
Sbjct: 254 TPPLPPESQIASPPHWGFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQK 313

Query: 305 QWCVAKPSVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNK 364
            WCVAKPSVP EILQQAMDYACGDGGADC+EISA+GSCFHPD+LVAHASYAFN+YWQKNK
Sbjct: 314 LWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNK 373

Query: 365 RSGGTCSFGGTAMIISSDPSFHHCRFVLS 383
           RSGGTCSFGGTAMIISSDPSF+HCRFVLS
Sbjct: 374 RSGGTCSFGGTAMIISSDPSFNHCRFVLS 399

[Close]

BLAST of Moc02g12540 vs. NCBI nr
Match: XP_004149701.2 (glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis sativus] >KAE8651912.1 hypothetical protein Csa_006335 [Cucumis sativus])

HSP 1 Score: 575.1 bits (1481), Expect = 4.5e-160
Identity = 298/382 (78.01%), Postives = 332/382 (86.91%), Query Frame = 0


Query: 11  FFTFLLGLTNAGQESIQVVKLDE-KTSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAE 70
           FF  LLGLT AGQESIQ VKLDE  +SGVFPA+S SELPVAVSVTD EL EVSSSVLMAE
Sbjct: 20  FFFLLLGLTTAGQESIQFVKLDEIASSGVFPAMSASELPVAVSVTDVELPEVSSSVLMAE 79

Query: 71  SWLRTFVLAHYPSTKITTIVVGNSSLCS------NNSNLVLLSLKNLFHSLTRWGLETQI 130
           SWLRTFVLAHYPSTKITTIVVGNSSLCS      NN ++VLLS+KNLF+SLTRWGLE QI
Sbjct: 80  SWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVLLSMKNLFYSLTRWGLENQI 139

Query: 131 KVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFISSK 190
           KVSTLF  DCFHS   +E+S ++M+KLV +FI++TNSTL+LKLPEN IS  E +SFIS+ 
Sbjct: 140 KVSTLFPKDCFHS---QEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFISTH 199

Query: 191 LEKIGFLELEKLNLITSVSNQRNPIGRKL-NFIEFD--DPLFTAEPPLPSDLARPPNPPE 250
             K GFL+L+K+NL+TSVS QRNP  RKL +F+E    +P FT+EPPLPSDLA PP PPE
Sbjct: 200 TNKFGFLKLKKVNLLTSVSKQRNPTNRKLSSFMELKEYEPFFTSEPPLPSDLATPPLPPE 259

Query: 251 SQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKP 310
           SQIASPP   FA+APE PPFVVPASPPMGF LPPCNP+QNAGAPFPQTGG+QK WCVAKP
Sbjct: 260 SQIASPPHWSFASAPESPPFVVPASPPMGFTLPPCNPNQNAGAPFPQTGGVQKLWCVAKP 319

Query: 311 SVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCS 370
           +VP EILQQAMDYACGDGGADC+EISA+GSCFHPD+LVAHASYAFN+YWQKNKRSGGTCS
Sbjct: 320 NVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCS 379

Query: 371 FGGTAMIISSDPSFHHCRFVLS 383
           FGGTAMIISSDPSF+HCRFVLS
Sbjct: 380 FGGTAMIISSDPSFNHCRFVLS 398

[Close]

BLAST of Moc02g12540 vs. NCBI nr
Match: XP_038900661.1 (glucan endo-1,3-beta-glucosidase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 569.7 bits (1467), Expect = 1.9e-158
Identity = 299/383 (78.07%), Postives = 330/383 (86.16%), Query Frame = 0


Query: 5   LLSSLLFFTFLLGLTNAGQESIQVVKLDE-KTSGVFPALSGSELPVAVSVTDAELAEVSS 64
           L     FFTFLLGLT AGQESIQ VKLDE  +SGVFPA+SGS+LPVAVSVTDAEL EVSS
Sbjct: 7   LFPFFFFFTFLLGLTTAGQESIQFVKLDEIISSGVFPAISGSDLPVAVSVTDAELPEVSS 66

Query: 65  SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCS------NNSNLVLLSLKNLFHSLTRW 124
           SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCS      N    VLLSLKNLF+SLTRW
Sbjct: 67  SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSTKNQDTNTLQFVLLSLKNLFYSLTRW 126

Query: 125 GLETQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMD 184
           GLE QIKVSTLF  DCFHS   +E+S ++M+KLV +FI++TNSTLTLKL EN IS  E +
Sbjct: 127 GLENQIKVSTLFPKDCFHS---QEESIQNMVKLVIEFIQSTNSTLTLKLTENVISLHETE 186

Query: 185 SFISSKLEKIGFLELEKLNLITSVSNQRNPIGRKL-NFIEFD--DPLFTAEPPLPSDLAR 244
           +FIS+ + K GFL+L K+NLITSV+NQRNPI RKL +F+E +  +P FT+EPPLPSDLA 
Sbjct: 187 NFISTHMNKFGFLKLHKVNLITSVTNQRNPISRKLSSFMELNEYEPFFTSEPPLPSDLAT 246

Query: 245 PPNPPESQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQ 304
           PP PPESQIASPP   FAAAPE PPFVVPASPPMGF+LPPCNPDQNAGAPFPQTG +QKQ
Sbjct: 247 PPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFSLPPCNPDQNAGAPFPQTGSVQKQ 306

Query: 305 WCVAKPSVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKR 364
           WCVAKPSVP EILQQAMDYACGDGGADC+EISA+GSCFHPDTLVAHASYAFN+YWQKNKR
Sbjct: 307 WCVAKPSVPTEILQQAMDYACGDGGADCREISAEGSCFHPDTLVAHASYAFNSYWQKNKR 366

Query: 365 SGGTCSFGGTAMIISSDPSFHHC 378
           +GGTCSFGGTAMIISSDPS + C
Sbjct: 367 NGGTCSFGGTAMIISSDPSKNLC 386

[Close]

BLAST of Moc02g12540 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.3e-21
Identity = 111/413 (26.88%), Postives = 175/413 (42.37%), Query Frame = 0


Query: 23  QESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAESWLRTFVLAHYPS 82
           Q +I+ V++ +  S V  A   + + + + V +++L   S S    ++WL+  VL +YP+
Sbjct: 49  QHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPT 108

Query: 83  TKITTIVVGNSSLCS---NNSNLVLLSLKNLFHSLTRWGLETQIKVSTLFSIDCF-HSDP 142
           TKIT I VG  S      N S+ V+ +++N+  +L + GL  +IKVST  S+     S P
Sbjct: 109 TKITYITVGAESTDDPHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFP 168

Query: 143 QE----EDSAEHMLKLVFDFIKATNSTLTLKL--------PENAISQDEMDSFISSKLEK 202
                   S  + L+ + +F+    S   + L          N +S D +    SS++  
Sbjct: 169 PSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVID 228

Query: 203 IGFLELEKLNLITSVSNQRNPIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPESQIASP 262
                L K N+  +  +        LNF      +   E   P+      +P E   AS 
Sbjct: 229 PNTGLLYK-NMFDAQVDALYYALTALNFRTI--KIMVTETGWPT----KGSPKEKAAASS 288

Query: 263 PQMGFAAAPELPPFV----VPASPPMG-----FALPPCN---------------PDQNAG 322
                  +  +   V     PA P        F+L   N               PDQ + 
Sbjct: 289 DNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSV 348

Query: 323 APFPQTG---------------GMQKQWCVAKPSVPVEILQQAMDYACGDGGADCKEISA 381
                TG               G    WC+A        L+ A+D+ACG G  DC  I  
Sbjct: 349 YQLDFTGKSNGFHSNSSGTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQP 408

[Close]

BLAST of Moc02g12540 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 104.0 bits (258), Expect = 3.9e-21
Identity = 107/416 (25.72%), Postives = 187/416 (44.95%), Query Frame = 0


Query: 6   LSSLLFFTFLLGLTNAGQESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAEVSSSV 65
           +S+LL  T L+    A  + +  V+L +    +  AL+ +++ V +SV + +L  + SS 
Sbjct: 52  VSNLLSPTELVKFLQA--QKVNHVRLYDADPELLKALAKTKVRVIISVPNNQLLAIGSSN 111

Query: 66  LMAESWLRTFVLAHYPSTKITTIVVGNSSLCS--NNSNLVLLSLKNLFHSLTRWGLETQI 125
             A SW+   V+A+YP T IT I VG+  L +  +++ L+L ++++L+++L    L TQI
Sbjct: 112 STAASWIGRNVVAYYPETLITAISVGDEVLTTVPSSAPLLLPAIESLYNALVASNLHTQI 171

Query: 126 KVST----LFSIDCFHSDPQEEDSAEHMLKL-VFDFIKATNSTLTLKL-PENAISQDE-- 185
           KVST       +D F       +   H + + +  F+  T S L + L P     Q++  
Sbjct: 172 KVSTPHAASIMLDTFPPSQAYFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYVYMQNKGV 231

Query: 186 -----------------------------MDSFISSKLEKIGFLELEKLNLITSVSNQRN 245
                                        +D+ + +    +  L +  + ++ + S   +
Sbjct: 232 VPLDNCLFEPLTPSKEMVDPNTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLVTESGWPS 291

Query: 246 PIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPESQIASPPQMGFAAAPEL-PPFVVPAS 305
               K  +   D+        +     R   P   ++ S   +      +L  P V  AS
Sbjct: 292 KGDSKEPYATIDNADTYNSNLIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRAPPVSEAS 351

Query: 306 PPM--GFALPPCNPDQNAGAPFPQTGGMQKQWCVAKPSVPVEILQQAMDYACGDGGADCK 365
             +  G + P      +    F       + +C+A   V  + LQ A+D+ACG G ++C 
Sbjct: 352 WGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCS 411

Query: 366 EISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCSFGGTAMIISSDPSFHHCRF 380
           EI    SC+ P+ +  HAS+AFN+Y+QK  R+ G+C F G AMI ++DPS   C F
Sbjct: 412 EIQPGESCYQPNNVKGHASFAFNSYYQKEGRASGSCDFKGVAMITTTDPSHGSCIF 465

[Close]

BLAST of Moc02g12540 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 8.6e-21
Identity = 107/428 (25.00%), Postives = 186/428 (43.46%), Query Frame = 0


Query: 25  SIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAESWLRTFVLAHYPSTK 84
           +I+ V++ +    V  A + + + + + V +A+L   +      ++WL   +L +YPSTK
Sbjct: 52  NIKFVRIYDANIDVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTK 111

Query: 85  ITTIVVG--NSSLCSNNSNLVLLSLKNLFHSLTRWGLETQIKVSTLFSIDCF-HSDPQEE 144
           IT+I VG   +    N + LVL +++N+  +L + GL+ +IK+S+  S+     S P   
Sbjct: 112 ITSISVGLEVTEAPDNATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSS 171

Query: 145 D--SAEH--MLKLVFDFIKATNSTL-------------TLKLP-ENAISQDE-------- 204
              S +H   LK + +F+    S               T K+P E A+ +          
Sbjct: 172 ASFSKKHSAFLKPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPAT 231

Query: 205 ---MDSFISSKLEKIGF----LELEKLNLITSVS-------------NQRNPIGRKLNFI 264
                +   ++L+ I F    +  + + ++ + S                N +    N I
Sbjct: 232 GLLYSNMFDAQLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLI 291

Query: 265 E--FDDPLFTAEPPLPSDLARPPNPPESQ---IASPPQMGFAAAPELPPFVVPASPPMGF 324
                DP   A+P    D+       E++   I S    G   A     + +  +     
Sbjct: 292 RHVIGDPGTPAKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTT 351

Query: 325 ALPPCNPDQNAGAPFPQT----------------GGMQKQWCVAKPSVPVEILQQAMDYA 383
            + P N      +P P +                GG  K+WC+A     V  LQ A+D+A
Sbjct: 352 PVSPTN-STTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWA 411

[Close]

BLAST of Moc02g12540 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 2.3e-18
Identity = 103/403 (25.56%), Postives = 175/403 (43.42%), Query Frame = 0


Query: 24  ESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAESWLRTFVLAHYPST 83
           ++I  V+L +    +  A + + + V +SV + +L  +S S   A +W+   V A+YP+T
Sbjct: 47  QNINRVRLYDADRSMLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPAT 106

Query: 84  KITTIVVGNSSLCS--NNSNLVLLSLKNLFHSLTRWGLETQIKVSTLFS----IDCF-HS 143
            ITTI VG+  L S  N +++++ +LK +  +L    L+ QIKVST  S    +D F  S
Sbjct: 107 NITTIAVGSEVLTSLTNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPS 166

Query: 144 DPQEEDSAEHMLKLVFDFIKATNSTLTLK-------LPENAISQDEMDSFISSKLEKIGF 203
                 + + ++  +  F+++T S L L        +  N +   +   F   +  K   
Sbjct: 167 QAFFNKTWDPVIVPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANK--- 226

Query: 204 LELEKLNLITSVSNQRNPIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPESQIASPPQM 263
            E    N +   +N  + I     F       FT  P + ++   P     S+  +  + 
Sbjct: 227 -EAVDANTLLHYTNVFDAIVDAAYF-AMSYLNFTNIPIVVTESGWPSKGGPSEHDATVEN 286

Query: 264 GFAAAPELPPFVV--------PASPPMGFALPPCNPDQNAGAPFPQTGGM---------- 323
                  L   V+        P +    +     N D   G    +  G+          
Sbjct: 287 ANTYNSNLIQHVINKTGTPKHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYT 346

Query: 324 ---------------QKQWCVAKPSVPVEILQQAMDYACGDGGADCKEISADGSCFHPDT 380
                           + +C+AK  V  ++LQ A+D+ACG G  DC  +    SC+ PD 
Sbjct: 347 LRLAGAGAILANDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDD 406

[Close]

BLAST of Moc02g12540 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 94.0 bits (232), Expect = 4.0e-18
Identity = 100/408 (24.51%), Postives = 172/408 (42.16%), Query Frame = 0


Query: 24  ESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAESWLRTFVLAHYPST 83
           + I+ ++L     G+  AL+ + + V +S+ + +L  +  S   A +W++  V+AHYP+T
Sbjct: 56  QEIRHIRLYNADPGLLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYPAT 115

Query: 84  KITTIVVGNSSL--CSNNSNLVLLSLKNLFHSLTRWGLETQIKVSTLFS----IDCF-HS 143
            IT + VG+  L   SN + +++ ++KN+  +L    L+  IKVST  S    +D F  S
Sbjct: 116 MITAVSVGSEVLTSLSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFPPS 175

Query: 144 DPQEEDSAEHMLKLVFDFIKATNSTLTLK-------LPENAISQDEMDSFISSKLEKIGF 203
                 S   ++  +  F+++TNS L +        +  N +   +   F      K   
Sbjct: 176 QAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNK--- 235

Query: 204 LELEKLNLITSVSNQRNPIGR----KLNFIEFDD-PLFTAEPPLPSDLARPPNPPESQIA 263
            E    N +   SN  + +       + F+ F + P+   E   PS      N P++ + 
Sbjct: 236 -EAVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPS--KGETNEPDATLD 295

Query: 264 SPPQ---------MGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTG------ 323
           +            +     P+ P   V       +     N D  AG      G      
Sbjct: 296 NANTYNSNLIRHVLNKTGTPKRPGIAVST-----YIYELYNEDTKAGLSEKNWGLFNANG 355

Query: 324 ------------------GMQKQWCVAKPSVPVEILQQAMDYACGDGGADCKEISADGSC 380
                                + +C A+     ++LQ A+D+ACG G  DC  I    +C
Sbjct: 356 EPVYVLRLTNSGSVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETC 415

[Close]

BLAST of Moc02g12540 vs. ExPASy TrEMBL
Match: A0A6J1D7Y7 (Glucan endo-1,3-beta-D-glucosidase OS=Momordica charantia OX=3673 GN=LOC111017866 PE=3 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 1.5e-214
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0


Query: 1   MKNKLLSSLLFFTFLLGLTNAGQESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAE 60
           MKNKLLSSLLFFTFLLGLTNAGQESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAE
Sbjct: 1   MKNKLLSSLLFFTFLLGLTNAGQESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAE 60

Query: 61  VSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSNLVLLSLKNLFHSLTRWGLE 120
           VSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSNLVLLSLKNLFHSLTRWGLE
Sbjct: 61  VSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSNLVLLSLKNLFHSLTRWGLE 120

Query: 121 TQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFI 180
           TQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFI
Sbjct: 121 TQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFI 180

Query: 181 SSKLEKIGFLELEKLNLITSVSNQRNPIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPE 240
           SSKLEKIGFLELEKLNLITSVSNQRNPIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPE
Sbjct: 181 SSKLEKIGFLELEKLNLITSVSNQRNPIGRKLNFIEFDDPLFTAEPPLPSDLARPPNPPE 240

Query: 241 SQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKP 300
           SQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKP
Sbjct: 241 SQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKP 300

Query: 301 SVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCS 360
           SVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCS
Sbjct: 301 SVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCS 360

Query: 361 FGGTAMIISSDPSFHHCRFVLS 383
           FGGTAMIISSDPSFHHCRFVLS
Sbjct: 361 FGGTAMIISSDPSFHHCRFVLS 382

[Close]

BLAST of Moc02g12540 vs. ExPASy TrEMBL
Match: A0A1S3C6A1 (Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo OX=3656 GN=LOC103497386 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 6.1e-163
Identity = 305/389 (78.41%), Postives = 336/389 (86.38%), Query Frame = 0


Query: 5   LLSSLLFFTFLLGLTNAGQESIQVVKLDE-KTSGVFPALSGSELPVAVSVTDAELAEVSS 64
           LL    FF FLLGLT AGQESIQ VKL+E  +SGVFPA+S SELPVAVS+TD EL EVSS
Sbjct: 14  LLFFFFFFFFLLGLTTAGQESIQFVKLEEIVSSGVFPAMSASELPVAVSLTDVELPEVSS 73

Query: 65  SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSNL-------VLLSLKNLFHSLTR 124
           SVLMAESWLRTFVLAHYPSTKI+TIVVGNSSLCSN +NL       VLLS+KNLF+SLTR
Sbjct: 74  SVLMAESWLRTFVLAHYPSTKISTIVVGNSSLCSNKNNLDTNNLHIVLLSMKNLFYSLTR 133

Query: 125 WGLETQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEM 184
           WGLE QIKVSTLF  DCFHS   +E+S ++M+KLV +FI++TNSTL+LKLPEN IS  E 
Sbjct: 134 WGLENQIKVSTLFPKDCFHS---QEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHET 193

Query: 185 DSFISSKLEKIGFLELEKLNLITSVSNQRNPIGRKL-NFIEFD--DPLFTAEPPLPSDLA 244
           +SFIS+   K GFL+L K+NL+TSVS QRNPI RKL +F+E    DP FT+EPPLPSDLA
Sbjct: 194 ESFISTHTNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYDPFFTSEPPLPSDLA 253

Query: 245 RPPNPPESQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQK 304
            PP PPESQIASPP  GFAAAPE PPFVVPASPPMGF LPPCNPDQNAGAPFPQTGG+QK
Sbjct: 254 TPPLPPESQIASPPHWGFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQK 313

Query: 305 QWCVAKPSVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNK 364
            WCVAKPSVP EILQQAMDYACGDGGADC+EISA+GSCFHPD+LVAHASYAFN+YWQKNK
Sbjct: 314 LWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNK 373

Query: 365 RSGGTCSFGGTAMIISSDPSFHHCRFVLS 383
           RSGGTCSFGGTAMIISSDPSF+HCRFVLS
Sbjct: 374 RSGGTCSFGGTAMIISSDPSFNHCRFVLS 399

[Close]

BLAST of Moc02g12540 vs. ExPASy TrEMBL
Match: A0A6J1EY45 (Glucan endo-1,3-beta-D-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111439476 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 1.2e-158
Identity = 293/379 (77.31%), Postives = 323/379 (85.22%), Query Frame = 0


Query: 11  FFTFLLGLTNAGQESIQVVKLDEK-TSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAE 70
           FF FLLGLT AGQESIQ +KLDEK +SGV P +S S+LPVAVSVTDAEL EVSSSVLMAE
Sbjct: 20  FFFFLLGLTTAGQESIQFLKLDEKISSGVSPPISASQLPVAVSVTDAELPEVSSSVLMAE 79

Query: 71  SWLRTFVLAHYPSTKITTIVVGNSSLCSNNSN---LVLLSLKNLFHSLTRWGLETQIKVS 130
           +WLR FVLAHYP+T ITTIVVGNSSLC+ ++N    VLLSLKNLF+SLTRWGLE QIKVS
Sbjct: 80  TWLRAFVLAHYPATNITTIVVGNSSLCNQDTNHLHSVLLSLKNLFYSLTRWGLENQIKVS 139

Query: 131 TLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFISSKLEK 190
           TLF  DCFHS P       +ML LV +FIK TNST TLKLP+N +S  E  SF+S+++ K
Sbjct: 140 TLFPDDCFHSKP------HNMLNLVIEFIKTTNSTFTLKLPQNVLSLHETQSFVSTRMAK 199

Query: 191 IGFLELEKLNLITSVSNQRNPIGRKL-NFIEFD--DPLFTAEPPLPSDLARPPNPPESQI 250
            GFL+L K+NLIT+VS QRNP  RKL +F+E +  +  FT+EPPLPSDLA PPNPPESQI
Sbjct: 200 FGFLKLNKVNLITTVSKQRNPFSRKLFSFMELNEYESFFTSEPPLPSDLAIPPNPPESQI 259

Query: 251 ASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKPSVP 310
           ASPP  GFAAAPE PPFVVPASPP+GF LPPCNPDQN GAPFPQTGG+QKQWCVAKPSVP
Sbjct: 260 ASPPHWGFAAAPESPPFVVPASPPLGFVLPPCNPDQNVGAPFPQTGGVQKQWCVAKPSVP 319

Query: 311 VEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCSFGG 370
            EILQQAMDYACG GGADC+EISADG CFHPDTLVAHASYAFN+YWQKNKRSGGTCSFGG
Sbjct: 320 AEILQQAMDYACGQGGADCREISADGDCFHPDTLVAHASYAFNSYWQKNKRSGGTCSFGG 379

Query: 371 TAMIISSDPSFHHCRFVLS 383
           TAMIISSDPSFHHCRFVLS
Sbjct: 380 TAMIISSDPSFHHCRFVLS 392

[Close]

BLAST of Moc02g12540 vs. ExPASy TrEMBL
Match: A0A6J1JGG8 (Glucan endo-1,3-beta-D-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111484177 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 4.3e-156
Identity = 293/388 (75.52%), Postives = 324/388 (83.51%), Query Frame = 0


Query: 3   NKLLSSLLF-FTFLLGLTNAGQESIQVVKL-DEKTSGVFPALSGSELPVAVSVTDAELAE 62
           N LL S  F F FLLGLT AGQESIQ +KL D+ +SGV P +S S+LP+AVSVTDAEL E
Sbjct: 6   NNLLCSFFFSFFFLLGLTTAGQESIQFLKLYDKISSGVSPPISASQLPIAVSVTDAELPE 65

Query: 63  VSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNNSN---LVLLSLKNLFHSLTRW 122
           VSSSVLMAE+WLR FVLAHYP+T ITTIVVGNSSLC+ ++N    VLLSLKNLF+SLTRW
Sbjct: 66  VSSSVLMAETWLRAFVLAHYPATSITTIVVGNSSLCNQDTNHLHSVLLSLKNLFYSLTRW 125

Query: 123 GLETQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMD 182
           GLE QIKVSTLF  DCFHS+P       +ML LV +FIK TNST TLKLP+N IS  E  
Sbjct: 126 GLENQIKVSTLFPEDCFHSNP------HNMLNLVTEFIKTTNSTFTLKLPQNVISLHETQ 185

Query: 183 SFISSKLEKIGFLELEKLNLITSVSNQRNPIGRKL-NFIEFD--DPLFTAEPPLPSDLAR 242
           SF+S+++ K G L+L K+NLIT VS QRNP  RKL +F+E +  +  FT+EPPLPSDLA 
Sbjct: 186 SFVSTRMAKFGILKLNKVNLITRVSKQRNPFSRKLFSFMELNEYEYFFTSEPPLPSDLAT 245

Query: 243 PPNPPESQIASPPQMGFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQ 302
           PPNPPESQIASPP  GFAAAPE PPFVVPASPP+ F LPPCNPDQN GAPFPQTGG+QKQ
Sbjct: 246 PPNPPESQIASPPHWGFAAAPESPPFVVPASPPLSFVLPPCNPDQNVGAPFPQTGGVQKQ 305

Query: 303 WCVAKPSVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKR 362
           WCVAKPSVP EILQQAMDYACG GGADC+EISADG CFHPDTLVAHASYAFN+YWQKNKR
Sbjct: 306 WCVAKPSVPAEILQQAMDYACGQGGADCREISADGDCFHPDTLVAHASYAFNSYWQKNKR 365

Query: 363 SGGTCSFGGTAMIISSDPSFHHCRFVLS 383
           SGGTCSFGGTAMIISSDPSFHHCRFVLS
Sbjct: 366 SGGTCSFGGTAMIISSDPSFHHCRFVLS 387

[Close]

BLAST of Moc02g12540 vs. ExPASy TrEMBL
Match: A0A5A7TQX1 (Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00660 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 3.4e-153
Identity = 287/364 (78.85%), Postives = 317/364 (87.09%), Query Frame = 0


Query: 21  AGQESIQVVKLDE-KTSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAESWLRTFVLAH 80
           AGQESIQ VKL+E  +SGVFPA+S SELPVAVS+TD EL EVSSSVLMAESWLRTFVLAH
Sbjct: 4   AGQESIQFVKLEEIVSSGVFPAMSASELPVAVSLTDVELPEVSSSVLMAESWLRTFVLAH 63

Query: 81  YPSTKITTIVVGNSSLCSNNSNL-------VLLSLKNLFHSLTRWGLETQIKVSTLFSID 140
           YPSTKI+TIVVGNSSLCSN +NL       VLLS+KNLF+SLTRWGLE QIKVSTLF  D
Sbjct: 64  YPSTKISTIVVGNSSLCSNKNNLDTNNLHIVLLSMKNLFYSLTRWGLENQIKVSTLFPKD 123

Query: 141 CFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFISSKLEKIGFLEL 200
           CFHS   +E+S ++M+KLV +FI++TNSTL+LKLPEN IS  E +SFIS+   K GFL+L
Sbjct: 124 CFHS---QEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFISTHTNKFGFLKL 183

Query: 201 EKLNLITSVSNQRNPIGRKL-NFIEFD--DPLFTAEPPLPSDLARPPNPPESQIASPPQM 260
            K+NL+TSVS QRNPI RKL +F+E    DP FT+EPPLPSDLA PP PPESQIASPP  
Sbjct: 184 NKVNLLTSVSKQRNPINRKLSSFMELKEYDPFFTSEPPLPSDLATPPLPPESQIASPPHW 243

Query: 261 GFAAAPELPPFVVPASPPMGFALPPCNPDQNAGAPFPQTGGMQKQWCVAKPSVPVEILQQ 320
           GFAAAPE PPFVVPASPPMGF LPPCNPDQNAGAPFPQTGG+QK WCVAKPSVP EILQQ
Sbjct: 244 GFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQ 303

Query: 321 AMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQKNKRSGGTCSFGGTAMIIS 374
           AMDYACGDGGADC+EISA+GSCFHPD+LVAHASYAFN+YWQKNKRSGGTCSFGGTAMIIS
Sbjct: 304 AMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIIS 363

[Close]

BLAST of Moc02g12540 vs. TAIR 10
Match: AT5G67460.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.3e-76
Identity = 172/391 (43.99%), Postives = 236/391 (60.36%), Query Frame = 0


Query: 5   LLSSLLFFTFLLGLTNAGQESIQVVKLDEKTSGVFPALSGSELPVAVSVT-DAELAEVSS 64
           LL   LF  F L    AGQES  +  L+   + +      ++L +AVSV+ +  + E+SS
Sbjct: 4   LLYIFLFIAF-LSFAGAGQESTPIEALN-LNNNLLQLPQTTDLDLAVSVSGNKTITEISS 63

Query: 65  SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLC---SNNSNLVLLSLKNLFHSLTRWGLE 124
           S++ AE+WL+T +L+ YPSTKITTIV+     C    ++S+LVL SLKN++HSLTRWGLE
Sbjct: 64  SIIKAETWLKTHILSRYPSTKITTIVIFFPDSCQTTQHSSDLVLSSLKNIYHSLTRWGLE 123

Query: 125 TQIKVSTLFSIDCFHSDPQEEDSAEHMLKLVFDFIKATNSTLTLKLPENAISQDEMDSFI 184
             IKVS+ FS  C ++    E     M K V  F+K  NST T+  P+N ++       +
Sbjct: 124 NNIKVSSGFSYQCLNNPKSSE-----MFKPVLIFLKTINSTFTINPPQNFLTSPHNHRDL 183

Query: 185 SSKLEKIGFLELEKLNLITSVSNQRNP---IGRKL-NFIEFDDPLFTAEPPLPSDLARPP 244
              +EK+G L   K+N +     Q +      R L + +       T+ P LPS     P
Sbjct: 184 LHSVEKLGSLSFNKVNFLNPEPEQESTTTMTRRNLRSLVNLSSKFTTSFPTLPS-----P 243

Query: 245 NPPESQIASPPQMGFAAAPELPPFVVPASPPM----GFALPPCNPDQNAGAPFP-QTGGM 304
           +P  S I S   +G  + P +P F  P+  PM    G +LPPC P   A +P P +   +
Sbjct: 244 SPETSPIHS--SIGSPSPPTIPYFPEPSQSPMESNQGISLPPCLPYHPAPSPQPVKKKNV 303

Query: 305 QKQWCVAKPSVPVEILQQAMDYACGDGGADCKEISADGSCFHPDTLVAHASYAFNNYWQK 364
           +  WCVAKPSV  E LQQ++D+ACG GGA+C EI   G C++PDT++AHASYAFN+YWQK
Sbjct: 304 EGLWCVAKPSVAAETLQQSLDFACGQGGANCDEIKPHGICYYPDTVMAHASYAFNSYWQK 363

Query: 365 NKRSGGTCSFGGTAMIISSDPSFHHCRFVLS 383
            KR+GGTCSFGGTAM+I++DPS+ HCRFVLS
Sbjct: 364 TKRNGGTCSFGGTAMLITTDPSYQHCRFVLS 380

[Close]

BLAST of Moc02g12540 vs. TAIR 10
Match: AT1G79480.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 136.7 bits (343), Expect = 3.8e-32
Identity = 78/170 (45.88%), Postives = 100/170 (58.82%), Query Frame = 0


Query: 220 PLFTAEPPLPSDLARPPNPPESQIASPPQMGFAAAPEL-PPFVVPA---------SPPMG 279
           P+    PP  S     PNPPE  + SPP+ G+   P L PP+  P          SP  G
Sbjct: 232 PITIPYPPESSS----PNPPEI-VPSPPESGYTPGPVLGPPYSEPGPSTPTGSIPSPSSG 291

Query: 280 FALPPCNPDQNAGAPFPQTGGMQKQWCVAKPSVPVEILQQAMDYACGDGGADCKEISADG 339
           F LPP         P P        WCVAKPSVP  I+Q+AM++ACG  GADC  I  +G
Sbjct: 292 F-LPPIVYPPPMAPPSPSVTPTSAYWCVAKPSVPDPIIQEAMNFACG-SGADCHSIQPNG 351

Query: 340 SCFHPDTLVAHASYAFNNYWQKNKRSGGTCSFGGTAMIISSDPSFHHCRF 380
            CF P+TL AHAS+A+N+YWQ+ K +GG+C+FGGT M+++ DPSF+ C F
Sbjct: 352 PCFKPNTLWAHASFAYNSYWQRTKSTGGSCTFGGTGMLVTVDPSFNGCHF 394

[Close]

BLAST of Moc02g12540 vs. TAIR 10
Match: AT1G79480.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 136.7 bits (343), Expect = 3.8e-32
Identity = 78/170 (45.88%), Postives = 100/170 (58.82%), Query Frame = 0


Query: 220 PLFTAEPPLPSDLARPPNPPESQIASPPQMGFAAAPEL-PPFVVPA---------SPPMG 279
           P+    PP  S     PNPPE  + SPP+ G+   P L PP+  P          SP  G
Sbjct: 231 PITIPYPPESSS----PNPPEI-VPSPPESGYTPGPVLGPPYSEPGPSTPTGSIPSPSSG 290

Query: 280 FALPPCNPDQNAGAPFPQTGGMQKQWCVAKPSVPVEILQQAMDYACGDGGADCKEISADG 339
           F LPP         P P        WCVAKPSVP  I+Q+AM++ACG  GADC  I  +G
Sbjct: 291 F-LPPIVYPPPMAPPSPSVTPTSAYWCVAKPSVPDPIIQEAMNFACG-SGADCHSIQPNG 350

Query: 340 SCFHPDTLVAHASYAFNNYWQKNKRSGGTCSFGGTAMIISSDPSFHHCRF 380
            CF P+TL AHAS+A+N+YWQ+ K +GG+C+FGGT M+++ DPSF+ C F
Sbjct: 351 PCFKPNTLWAHASFAYNSYWQRTKSTGGSCTFGGTGMLVTVDPSFNGCHF 393

[Close]

BLAST of Moc02g12540 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 123.2 bits (308), Expect = 4.4e-28
Identity = 113/422 (26.78%), Postives = 180/422 (42.65%), Query Frame = 0


Query: 24  ESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAESWLRTFVLAHYPST 83
           + I  VK+ +    V  ALSGS + V V + +  L   +     A SW++  V A++PST
Sbjct: 49  QGITRVKIFDADPSVLKALSGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPST 108

Query: 84  KITTIVVGNSSLCS--NNSNLVLLSLKNLFHSLTRWGLETQIKVSTLFSIDCF-HSDPQE 143
           +I +I VGN       N ++ ++ +++N+  +L  + L + IK+S+  ++    +S P  
Sbjct: 109 QIESIAVGNEVFVDTHNTTSFLIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSS 168

Query: 144 EDS-----AEHMLKLVFDFIKATNSTLTLK--------------------LPENAISQDE 203
             S      + ++K + DF++ T S L +                     L EN    D 
Sbjct: 169 SGSFRPELIDSVIKPMLDFLRETGSRLMINVYPFFAYEGNSDVIPLDYALLRENPGMVDS 228

Query: 204 ---------MDSFISSKLEKIGFLELEKLNLITSVS-----NQRNPIGRKL-NFIEFDDP 263
                     D+ I +    +  L+ + + +I + +        N +G  L N   ++  
Sbjct: 229 GNGLRYFNLFDAQIDAVFAAMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGN 288

Query: 264 LFTAEPPLPSDLARPPNPPESQI------------ASPPQMGFAAAPELPPFVVPASPP- 323
           L            RP       +             S    G     E   + +P +   
Sbjct: 289 LIRRILTRGGTPLRPKADLTVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEG 348

Query: 324 -----MGFALPPCNPDQNAGAPFPQTGGMQKQ-----WCVAKPSVPVEILQQAMDYACGD 380
                 G   P    DQ      P +GG+ K      WCVA      E LQ  +DYACG+
Sbjct: 349 LKHYRDGGHTPVTGGDQVTKP--PMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGE 408

[Close]

BLAST of Moc02g12540 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.7e-27
Identity = 108/411 (26.28%), Postives = 186/411 (45.26%), Query Frame = 0


Query: 24  ESIQVVKLDEKTSGVFPALSGSELPVAVSVTDAELAEVSSSVLMAESWLRTFVLAHYPST 83
           + I  +K+ +    V  AL+ S++ V V++ +  L+  +S    A++W++T ++ ++P+T
Sbjct: 49  QGINRIKIFDTDKNVLTALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPAT 108

Query: 84  KITTIVVGNSSLCSNNSNLVLL-SLKNLFHSLTRWGLETQIKVSTLFSIDCF-HSDPQEE 143
           +I  I VGN           L+ ++KN+  SL ++ L+  IK+S+  ++    +S P   
Sbjct: 109 EIEAIAVGNEVFVDPTITPYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSS 168

Query: 144 DS-----AEHMLKLVFDFIKATNSTLTLK------------------------------- 203
            S      E ++K +   ++ T+S L +                                
Sbjct: 169 GSFKPELIEPVVKPMLALLQQTSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSG 228

Query: 204 --LPENAISQDEMDSFISSKLEKIGFLELEKLNLIT---SVSNQRNPIGRKLNFIEFDDP 263
             L  N++   ++D+ + + L  +GF  ++ +   T   SV ++      + N   ++  
Sbjct: 229 TGLKYNSLFDAQIDA-VYAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAG 288

Query: 264 LFTAEPPLPSDLARPPNP---------PESQIASPP---QMGFAAAPELPPFVVPASPPM 323
           L            RP  P          E+Q   P      G     E   + VP +   
Sbjct: 289 LVKRVLTGKGTPLRPTEPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFTKK- 348

Query: 324 GFALPPCNPDQNAGAPFPQTGGMQKQWCVAKPSVPVEILQQAMDYACGDGGADCKEISAD 380
             +  P N ++    P    G     WCV+   V  E LQ+A+DYACG+GGADC+ I   
Sbjct: 349 --STTPVNGNRGK-VPVTHEG---HTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPG 408

The following BLAST results are available for this feature:

BLAST of Moc02g12540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149437.1	3.2e-214	100.00	glucan endo-1,3-beta-glucosidase 13 [Momordica charantia]	[more]
XP_038900662.1	3.0e-164	79.12	glucan endo-1,3-beta-glucosidase 1 isoform X2 [Benincasa hispida]	[more]
XP_008457775.1	1.3e-162	78.41	PREDICTED: glucan endo-1,3-beta-glucosidase 13 [Cucumis melo]	[more]
XP_004149701.2	4.5e-160	78.01	glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis sativus] >KAE8651912.1 h...	[more]
XP_038900661.1	1.9e-158	78.07	glucan endo-1,3-beta-glucosidase 1 isoform X1 [Benincasa hispida]	[more]

BLAST of Moc02g12540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJU9	1.3e-21	26.88	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
O65399	3.9e-21	25.72	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q8VYE5	8.6e-21	25.00	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9ZU91	2.3e-18	25.56	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q9C7U5	4.0e-18	24.51	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]

BLAST of Moc02g12540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7Y7	1.5e-214	100.00	Glucan endo-1,3-beta-D-glucosidase OS=Momordica charantia OX=3673 GN=LOC11101786...	[more]
A0A1S3C6A1	6.1e-163	78.41	Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo OX=3656 GN=LOC103497386 PE=3 ...	[more]
A0A6J1EY45	1.2e-158	77.31	Glucan endo-1,3-beta-D-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111439476...	[more]
A0A6J1JGG8	4.3e-156	75.52	Glucan endo-1,3-beta-D-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111484177 P...	[more]
A0A5A7TQX1	3.4e-153	78.85	Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Moc02g12540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67460.1	3.3e-76	43.99	O-Glycosyl hydrolases family 17 protein	[more]
AT1G79480.1	3.8e-32	45.88	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G79480.2	3.8e-32	45.88	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G05790.1	4.4e-28	26.78	O-Glycosyl hydrolases family 17 protein	[more]
AT4G26830.1	1.7e-27	26.28	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 294..379 e-value: 4.2E-49 score: 179.1
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 294..366 e-value: 1.4E-22 score: 80.2
None	No IPR available	GENE3D	1.20.58.1040		coord: 290..381 e-value: 1.5E-11 score: 46.6
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 19..180 e-value: 1.5E-24 score: 88.5
None	No IPR available	PANTHER	PTHR32227:SF237	GB\|AAD30228.1	coord: 5..379
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 26..153 e-value: 1.9E-12 score: 47.4
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 5..379

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g12540.1	Moc02g12540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds