Homology
BLAST of Moc02g12450 vs. NCBI nr
Match:
XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])
HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0
Query: 1 MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA 60
MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA
Sbjct: 1 MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA 60
Query: 61 EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL 120
EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL
Sbjct: 61 EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL 120
Query: 121 IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL 180
IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL
Sbjct: 121 IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL 180
Query: 181 LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG 240
LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG
Sbjct: 181 LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG 240
Query: 241 DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA 300
DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA
Sbjct: 241 DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA 300
Query: 301 NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ 360
NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ
Sbjct: 301 NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ 360
Query: 361 PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS 420
PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS
Sbjct: 361 PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS 420
Query: 421 EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS 480
EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS
Sbjct: 421 EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS 480
Query: 481 NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE 540
NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE
Sbjct: 481 NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE 540
Query: 541 RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI 587
RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI
Sbjct: 541 RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI 586
BLAST of Moc02g12450 vs. NCBI nr
Match:
XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])
HSP 1 Score: 800.0 bits (2065), Expect = 1.3e-227
Identity = 389/497 (78.27%), Postives = 445/497 (89.54%), Query Frame = 0
Query: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
MDIL LSISLSLFSL + F FL R R+NLPPSP F LP++GHLHLVKHPVH+T H+LSH
Sbjct: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
YGHVFSLR+GSR+VVVVSSPSAVQECFTKNDIVLANRPLLKSGK LAYNHTT+AV+PYG
Sbjct: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
EHWRNLRRI TLEIFST+RLN+FLGIREDEV+NL+ KL +S +EF AVEL+ ML D TF
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
NIV+RM+AGKKYY +E QSKKFREMV ++LA+ G N GDF+P+WNWIDPTG+EKR
Sbjct: 181 NIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 240
Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
I+K+G+RSDELLQEL+DEIRNQ+ G NTMIDHLL LQ +PEY+SDQIIKGL Q++LLAG
Sbjct: 241 IIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAG 300
Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
IDTAA+TLEWALS+LLNNPEVL+KA+AEID F+G++RLV+E DLSSL YLQG+I ETLRL
Sbjct: 301 IDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRL 360
Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
NPAAPLL+PHCAS DCKI GYD+PRDTIILIN WAIHRD N+WEDAT+FKPERH NT+ G
Sbjct: 361 NPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTI-G 420
Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
VDSYKLLPFG GRR+CPG+GMAQRVVGLTLASLIQ FEWER+++SLVDMTEG+GLTMPKA
Sbjct: 421 VDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKA 480
Query: 561 QPLVAKCKPRPILKAIL 578
Q LVAKCKPRPI+K IL
Sbjct: 481 QHLVAKCKPRPIMKLIL 491
BLAST of Moc02g12450 vs. NCBI nr
Match:
XP_022947035.1 (cytochrome P450 81F3 [Cucurbita moschata])
HSP 1 Score: 767.7 bits (1981), Expect = 7.3e-218
Identity = 369/499 (73.95%), Postives = 433/499 (86.77%), Query Frame = 0
Query: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
MDILLLSI L L SL F+FL P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60
Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
+GHV SLR+GSRLVVV+SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLAVSPYG
Sbjct: 61 KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120
Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
EHWRNLRRI +LEIFST+RLN+F+GIRE+EVK LLRKL GD +EFR VEL+ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180
Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
N+V+ M+ G+++Y DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240
Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
IM + R DELLQEL+DE R+ KDGGNTMI HLL LQ +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300
Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
IDT+A+TLEWALS LLNNP+VLKKARAEID IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRL 360
Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
NPAAPLLVPHCA +DCKIEGYD+PRDTI+LIN WAIHRD N+WED T+FKPERH +T G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDT-SG 420
Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480
Query: 561 QPLVAKCKPRPILKAILGV 580
QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493
BLAST of Moc02g12450 vs. NCBI nr
Match:
XP_023007415.1 (cytochrome P450 81F3-like [Cucurbita maxima])
HSP 1 Score: 765.8 bits (1976), Expect = 2.8e-217
Identity = 367/499 (73.55%), Postives = 435/499 (87.17%), Query Frame = 0
Query: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
MDILLLSI L L SL F+FL P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60
Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
+GHV SLR+GSRLVV++SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLA+SPYG
Sbjct: 61 KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120
Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
EHWRNLRRI +LEIFSTSRLNLFLGIR++EVK LLRKL GD L+EFR VE++ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180
Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
N+V+ M+ G+++Y DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240
Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
IM + R DELLQEL+DE R+ KDGGN+MI HLL LQ +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300
Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
IDT+A+TLEWALS LLNNP+VLKKARAEID IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRL 360
Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
NPAAPLLVPHCA +DCKIEGYD+PRDTI+LINAWAIHRD ++WED T+FKPERH ++ G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDS-SG 420
Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480
Query: 561 QPLVAKCKPRPILKAILGV 580
QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493
BLAST of Moc02g12450 vs. NCBI nr
Match:
XP_023532689.1 (cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.1 cytochrome P450 81F3-like isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 765.8 bits (1976), Expect = 2.8e-217
Identity = 368/499 (73.75%), Postives = 434/499 (86.97%), Query Frame = 0
Query: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
MDILLLSI L L SL F+FL P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHTLSQ 60
Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
+GHV SLR+GSRLVVV+SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLA+SPYG
Sbjct: 61 KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120
Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
EHWRNLRRI +LEIFST+RLN+F+GIRE+EVK LLRKL G L+E R VEL+ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGGWLEEGREVELEPMLSDLTF 180
Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
N+V+ M+ G+++Y DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240
Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
IM + R DE+LQEL+DE R+ KDGGNTMI HLL LQ +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDEVLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300
Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
IDT+A+TLEWALSQLLNNP+VLKKARAEID IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSQLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRL 360
Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
NPAAPLLVPHCA +DCKIEGYD+PRDTI+LINAWAIHRD N+WED T+FKPERH +T G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPNLWEDPTAFKPERHGDT-SG 420
Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480
Query: 561 QPLVAKCKPRPILKAILGV 580
QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493
BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 580.1 bits (1494), Expect = 2.8e-164
Identity = 285/497 (57.34%), Postives = 369/497 (74.25%), Query Frame = 0
Query: 83 ILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNY 142
++LLSISL LF + RRNLPPSP LP+IGHLHL+ +HR+L+ LS Y
Sbjct: 13 VVLLSISLKLFP--------VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 72
Query: 143 GHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEH 202
G VFSL+ G+RLV+VVSSP+A +ECFTKNDIV ANRPL GK++ YN+TT+ SPYGEH
Sbjct: 73 GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 132
Query: 203 WRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNI 262
WRNLRR++ +EIFS LN FL IREDEVK LL L S Q+F VE+ S L + +FN+
Sbjct: 133 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNV 192
Query: 263 VVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIM 322
+RM+AGK+Y+G+D+ D ++K FR ++ E+ + G +N GDFVP WID YEK++
Sbjct: 193 TMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVS 252
Query: 323 KIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGID 382
KI + D LQ L+ E R K+ TMIDHLLSLQ +QPEY++DQIIKG+ +LLLAG D
Sbjct: 253 KISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTD 312
Query: 383 TAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLNP 442
T+A+T+EWA+S LLN+PE L+KAR EI+ +G +RL+ E DL L+YL +I+ET RL P
Sbjct: 313 TSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCP 372
Query: 443 AAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGV- 502
AAP+LVPH +S+DCK++GYD+P+ TI+L+NAWAIHRD W++ T FKPERH GGV
Sbjct: 373 AAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH----GGVE 432
Query: 503 -DSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 562
+ KL+PFG+GRR+CPG G+AQRVVGLTL +LIQCFEW+R+ + +DM EG GLTMPKA
Sbjct: 433 LEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKA 492
Query: 563 QPLVAKCKPRPILKAIL 578
QPL A CKPR IL ++
Sbjct: 493 QPLEALCKPRNILHKVV 495
BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 535.8 bits (1379), Expect = 6.1e-151
Identity = 277/501 (55.29%), Postives = 357/501 (71.26%), Query Frame = 0
Query: 83 ILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNY 142
++L SI SL L I F F L+P ++NLPPSP LP+IGHL L+K P+HRTL S
Sbjct: 8 VVLYSI-FSLIFLIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67
Query: 143 GH-----VFSLRYGSRLVVVVSSPS-AVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAV 202
H V SLR GSRLV VVSS A +ECF KND+VLANRP + GK + YN+T +
Sbjct: 68 DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127
Query: 203 SPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLL 262
+PYG+HWRNLRR+ T+EIFST RLN FL +R DEV+ L+ +L + + VEL ML+
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 187
Query: 263 DHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTG 322
D TFN ++RM+ GK+YYGE+ DE ++K+ R++V ++ AN + N D+VP+ +
Sbjct: 188 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SS 247
Query: 323 YEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQIL 382
YE R+ K+GE +D+ LQ L+D+ R Q++ G TMIDHLL LQ + EY++DQIIKG+ I+
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 307
Query: 383 LLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITE 442
++AG +T+A+TLEWALS LLN+P+V+ KAR EID+ +G DRL+ E DLS L YL+ ++ E
Sbjct: 308 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 367
Query: 443 TLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHAN 502
TLRL+PA PLLVPH ASEDCKI YD+PR T +L+NAWAIHRD N W+D SFKPER
Sbjct: 368 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 427
Query: 503 TMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLT 562
++ KLL FGLGRRACPG G+AQR+VGL L SLIQCFEWERV + VDM EG G T
Sbjct: 428 E---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNT 487
Query: 563 MPKAQPLVAKCKPRPILKAIL 578
+PKA PL A CK RP L I+
Sbjct: 488 VPKAIPLKAICKARPFLHKII 501
BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 535.0 bits (1377), Expect = 1.0e-150
Identity = 268/500 (53.60%), Postives = 359/500 (71.80%), Query Frame = 0
Query: 81 MDIL-LLSISLSLFSLFIFFHFLLRPYR-RNLPPSPFFRLPLIGHLHLVKHPVHRTLHKL 140
M+IL LLS S+ +LF F+ ++R + +NLPP P LP+IG+LH +K P+HRT L
Sbjct: 1 MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGL 60
Query: 141 SHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSP 200
S YGHVFSL +GSRLVVVVSS S Q+CFTKND+VLANRP SGK++ YN+TTL +
Sbjct: 61 SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120
Query: 201 YGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDH 260
YGEHWRNLRRI+ L++ S R+N F GIR DE + L+ +L DS F +EL S L D
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 180
Query: 261 TFNIVVRMIAGKKYYGE--DLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTG 320
TFN ++RMI+GK+YYGE D D ++ +FR+MV E+L G N DF+PL ++D
Sbjct: 181 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 240
Query: 321 YEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQIL 380
EKR+ I ++D L+ L++E R +K+ NTMIDHLL+LQ++QPEY++DQIIKGLA +
Sbjct: 241 LEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 300
Query: 381 LLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITE 440
LLAG D++A+TLEW++S LLN+PEVLKK + E+D +G+DRLV E DL L+YL+ VI E
Sbjct: 301 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 360
Query: 441 TLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHAN 500
TLRL APLL+PH S++C I GY +P+DTI+LINAWAIHRD +W +AT+FKPER
Sbjct: 361 TLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERF-E 420
Query: 501 TMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLT 560
G ++ KL+ FG+GRRACPG G+A R + +TLA LIQCF+W+ +N +D+ E +G T
Sbjct: 421 KKGELE--KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFT 480
Query: 561 MPKAQPLVAKCKPRPILKAI 577
+ K PL A CK RP++ +
Sbjct: 481 LTKLVPLKAMCKSRPVINKV 496
BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 528.9 bits (1361), Expect = 7.5e-149
Identity = 269/498 (54.02%), Postives = 349/498 (70.08%), Query Frame = 0
Query: 81 MDILLLSISLSLFSLFIFFHFLLRPYR--RNLPPSPFFRLPLIGHLHLVKHPVHRTLHKL 140
M LS+ +SLF L I R +NLPP P LP+IG+LH +K P+H T H L
Sbjct: 1 MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQC-LPIIGNLHQLKQPLHHTFHTL 60
Query: 141 SHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSP 200
S YG +FSL +GSRLVVVVSS + QECFTKNDIVLANRP +GK++ YN+TT+A SP
Sbjct: 61 SQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSP 120
Query: 201 YGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDH 260
YG+HWRNLRRI ++EI S+ RLN FL IR DE+ L++KL S F VEL M +
Sbjct: 121 YGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEM 180
Query: 261 TFNIVVRMIAGKKYYGE--DLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTG 320
TFN ++RM++GK+YYG D+ D +++ FR ++KE+++ GG N+GDF+ W D G
Sbjct: 181 TFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDG 240
Query: 321 YEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQIL 380
EKR+ KI +R+D LQ L+DE R K NTMIDHLL+ Q +QPEY++DQIIKGL ++
Sbjct: 241 LEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 300
Query: 381 LLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITE 440
LLAG DT+++T+EWA+S LLN+PE++KKA+ E+D IG DR V E D+S L YLQ ++ E
Sbjct: 301 LLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYE 360
Query: 441 TLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHAN 500
TLRL+ AAPLLVPH +SED + GY+IP++TI+++NAW IHRD N+W D T FKPER
Sbjct: 361 TLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK 420
Query: 501 TMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLT 560
G V+ KLL FGLGRRACPG ++QR GLTL LIQCFEW+R+ +DM E +G+T
Sbjct: 421 E-GEVN--KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGIT 480
Query: 561 MPKAQPLVAKCKPRPILK 575
K L A CK R LK
Sbjct: 481 AGKKTSLNAMCKVRHPLK 494
BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match:
Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)
HSP 1 Score: 525.8 bits (1353), Expect = 6.3e-148
Identity = 267/509 (52.46%), Postives = 354/509 (69.55%), Query Frame = 0
Query: 74 ISNTKRRMDILLLSISLSLFSLFIFFHFLL--RPYRRNLPPSPFFRLPLIGHLHLVKHPV 133
+S+TK M+ + L +SL F +F+ L RP + NLPPSP P+IGHLHL+K P+
Sbjct: 1 MSSTKTIMETIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPL 60
Query: 134 HRTLHKLSH--NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAY 193
HR LS N +FSL GSRLV VVSS + +ECFTKND+VLANRP GK + Y
Sbjct: 61 HRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGY 120
Query: 194 NHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAV 253
N TT+ + YG+ WRNLRRI T+EIFS+ RLN F+ IR+DE++ L+ L +S F V
Sbjct: 121 NSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKV 180
Query: 254 ELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLW 313
E+ + + T N ++RM+AGK++YG+ ++ ++K R+++ E++ +GG N D+ P+
Sbjct: 181 EMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPIL 240
Query: 314 NWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQII 373
++ T YEK + K+ R DE LQ LV+E R +K GNTMIDHLLSLQ TQP+Y++D II
Sbjct: 241 RYV--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVII 300
Query: 374 KGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSY 433
KG+ +++LAG DT+A TLEWA+S LLN+PEVL+KA+ EID IG DRLV E D+ L Y
Sbjct: 301 KGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPY 360
Query: 434 LQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSF 493
LQ +++ETLRL P AP+L+PH ASEDC ++GYD+PR TIIL+NAWAIHRD +WE+ F
Sbjct: 361 LQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKF 420
Query: 494 KPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDM 553
KPER G D KL+PFG+GRR+CPG G+AQR+V L L SL+QCFEWERV +DM
Sbjct: 421 KPERFEKK--GEDK-KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDM 480
Query: 554 TEGE-GLTMPKAQPLVAKCKPRPILKAIL 578
E E G TM KA L A CK RPI+ +L
Sbjct: 481 RESEKGTTMRKATSLQAMCKARPIVHKVL 504
BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match:
A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)
HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0
Query: 1 MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA 60
MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA
Sbjct: 1 MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA 60
Query: 61 EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL 120
EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL
Sbjct: 61 EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL 120
Query: 121 IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL 180
IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL
Sbjct: 121 IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL 180
Query: 181 LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG 240
LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG
Sbjct: 181 LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG 240
Query: 241 DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA 300
DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA
Sbjct: 241 DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA 300
Query: 301 NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ 360
NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ
Sbjct: 301 NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ 360
Query: 361 PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS 420
PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS
Sbjct: 361 PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS 420
Query: 421 EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS 480
EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS
Sbjct: 421 EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS 480
Query: 481 NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE 540
NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE
Sbjct: 481 NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE 540
Query: 541 RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI 587
RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI
Sbjct: 541 RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI 586
BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match:
A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)
HSP 1 Score: 800.0 bits (2065), Expect = 6.4e-228
Identity = 389/497 (78.27%), Postives = 445/497 (89.54%), Query Frame = 0
Query: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
MDIL LSISLSLFSL + F FL R R+NLPPSP F LP++GHLHLVKHPVH+T H+LSH
Sbjct: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
YGHVFSLR+GSR+VVVVSSPSAVQECFTKNDIVLANRPLLKSGK LAYNHTT+AV+PYG
Sbjct: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
EHWRNLRRI TLEIFST+RLN+FLGIREDEV+NL+ KL +S +EF AVEL+ ML D TF
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
NIV+RM+AGKKYY +E QSKKFREMV ++LA+ G N GDF+P+WNWIDPTG+EKR
Sbjct: 181 NIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 240
Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
I+K+G+RSDELLQEL+DEIRNQ+ G NTMIDHLL LQ +PEY+SDQIIKGL Q++LLAG
Sbjct: 241 IIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAG 300
Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
IDTAA+TLEWALS+LLNNPEVL+KA+AEID F+G++RLV+E DLSSL YLQG+I ETLRL
Sbjct: 301 IDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRL 360
Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
NPAAPLL+PHCAS DCKI GYD+PRDTIILIN WAIHRD N+WEDAT+FKPERH NT+ G
Sbjct: 361 NPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTI-G 420
Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
VDSYKLLPFG GRR+CPG+GMAQRVVGLTLASLIQ FEWER+++SLVDMTEG+GLTMPKA
Sbjct: 421 VDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKA 480
Query: 561 QPLVAKCKPRPILKAIL 578
Q LVAKCKPRPI+K IL
Sbjct: 481 QHLVAKCKPRPIMKLIL 491
BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match:
A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)
HSP 1 Score: 767.7 bits (1981), Expect = 3.5e-218
Identity = 369/499 (73.95%), Postives = 433/499 (86.77%), Query Frame = 0
Query: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
MDILLLSI L L SL F+FL P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60
Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
+GHV SLR+GSRLVVV+SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLAVSPYG
Sbjct: 61 KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120
Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
EHWRNLRRI +LEIFST+RLN+F+GIRE+EVK LLRKL GD +EFR VEL+ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180
Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
N+V+ M+ G+++Y DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240
Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
IM + R DELLQEL+DE R+ KDGGNTMI HLL LQ +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300
Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
IDT+A+TLEWALS LLNNP+VLKKARAEID IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRL 360
Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
NPAAPLLVPHCA +DCKIEGYD+PRDTI+LIN WAIHRD N+WED T+FKPERH +T G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDT-SG 420
Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480
Query: 561 QPLVAKCKPRPILKAILGV 580
QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493
BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match:
A0A6J1L2W8 (cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1)
HSP 1 Score: 765.8 bits (1976), Expect = 1.3e-217
Identity = 367/499 (73.55%), Postives = 435/499 (87.17%), Query Frame = 0
Query: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
MDILLLSI L L SL F+FL P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60
Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
+GHV SLR+GSRLVV++SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLA+SPYG
Sbjct: 61 KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120
Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
EHWRNLRRI +LEIFSTSRLNLFLGIR++EVK LLRKL GD L+EFR VE++ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180
Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
N+V+ M+ G+++Y DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240
Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
IM + R DELLQEL+DE R+ KDGGN+MI HLL LQ +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300
Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
IDT+A+TLEWALS LLNNP+VLKKARAEID IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRL 360
Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
NPAAPLLVPHCA +DCKIEGYD+PRDTI+LINAWAIHRD ++WED T+FKPERH ++ G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDS-SG 420
Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480
Query: 561 QPLVAKCKPRPILKAILGV 580
QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493
BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match:
A0A6J1D797 (isoflavone 3'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017936 PE=3 SV=1)
HSP 1 Score: 763.8 bits (1971), Expect = 5.1e-217
Identity = 365/498 (73.29%), Postives = 434/498 (87.15%), Query Frame = 0
Query: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
M+ LLLSI SLFSL I FL RP R NLPPSP F LPLIGH+HLVKHPVHRT HK+S
Sbjct: 1 MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60
Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
+G +FSLR+GSRL+VVVSS SAVQECFTKNDIVLANRP++KSGK+LAYN+TTL SPYG
Sbjct: 61 KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120
Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
EHWRNLRRIS LE+ STSRLN F+GIREDE+K L+ +L GDSL+EF VE++ + LD TF
Sbjct: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180
Query: 261 NIVVRMIAGKKYYGEDL-RDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEK 320
+IV+RM+AGKKYYG+D+ D QS+KFRE+V +ILA+ + N GDF+P+WN IDP+G EK
Sbjct: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240
Query: 321 RIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLA 380
RIMK+G+R+DEL Q L+DEIRNQ +GGNTMIDHLL LQNTQPEY+SDQ+IKG+ Q ++LA
Sbjct: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300
Query: 381 GIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLR 440
GIDT+A+T+EWALS LLNNP+VLKKAR EID+ IG++R+V+E DLSSLSYLQG+I+ETLR
Sbjct: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360
Query: 441 LNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG 500
L P P+L+PHCASEDCK+EGYDIPRDTI+L+NAWAIHRD N+WEDA SFKPERH N +
Sbjct: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAI- 420
Query: 501 GVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPK 560
++Y+LLPFG+GRRACPGMGMAQRVVGLTLASLIQCFEW+RV+SSLVDMTEGEG TMPK
Sbjct: 421 RPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPK 480
Query: 561 AQPLVAKCKPRPILKAIL 578
AQPL+AKCKP PI+K IL
Sbjct: 481 AQPLIAKCKPHPIMKVIL 497
BLAST of Moc02g12450 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 535.8 bits (1379), Expect = 4.3e-152
Identity = 277/501 (55.29%), Postives = 357/501 (71.26%), Query Frame = 0
Query: 83 ILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNY 142
++L SI SL L I F F L+P ++NLPPSP LP+IGHL L+K P+HRTL S
Sbjct: 8 VVLYSI-FSLIFLIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67
Query: 143 GH-----VFSLRYGSRLVVVVSSPS-AVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAV 202
H V SLR GSRLV VVSS A +ECF KND+VLANRP + GK + YN+T +
Sbjct: 68 DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127
Query: 203 SPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLL 262
+PYG+HWRNLRR+ T+EIFST RLN FL +R DEV+ L+ +L + + VEL ML+
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 187
Query: 263 DHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTG 322
D TFN ++RM+ GK+YYGE+ DE ++K+ R++V ++ AN + N D+VP+ +
Sbjct: 188 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SS 247
Query: 323 YEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQIL 382
YE R+ K+GE +D+ LQ L+D+ R Q++ G TMIDHLL LQ + EY++DQIIKG+ I+
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 307
Query: 383 LLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITE 442
++AG +T+A+TLEWALS LLN+P+V+ KAR EID+ +G DRL+ E DLS L YL+ ++ E
Sbjct: 308 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 367
Query: 443 TLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHAN 502
TLRL+PA PLLVPH ASEDCKI YD+PR T +L+NAWAIHRD N W+D SFKPER
Sbjct: 368 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 427
Query: 503 TMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLT 562
++ KLL FGLGRRACPG G+AQR+VGL L SLIQCFEWERV + VDM EG G T
Sbjct: 428 E---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNT 487
Query: 563 MPKAQPLVAKCKPRPILKAIL 578
+PKA PL A CK RP L I+
Sbjct: 488 VPKAIPLKAICKARPFLHKII 501
BLAST of Moc02g12450 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 533.5 bits (1373), Expect = 2.2e-151
Identity = 267/490 (54.49%), Postives = 355/490 (72.45%), Query Frame = 0
Query: 84 LLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH--N 143
L+ SI + SL L R + NLPPSP + LP+IGHL L+K P+HRT LS N
Sbjct: 6 LIFSILFVVLSLIYLIGKLKR--KPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 65
Query: 144 YGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGE 203
+FSLR G+RLV V SS S +ECFTKND+VLANRP K +AY++TT+ + YG+
Sbjct: 66 NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 125
Query: 204 HWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFN 263
HWRNLRRI ++EIFS RLN FL IR+DE++ L+ +L + QEF V++ SML D TFN
Sbjct: 126 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFN 185
Query: 264 IVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRI 323
++RM+AGK+YYG+ + D+ ++K+ R+++ +++A G N D++P+ + + YE R+
Sbjct: 186 NILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRV 245
Query: 324 MKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGI 383
K+ R DE LQ LVDE R K+ GNTMIDHLL+LQ +QP+YF+D+IIKG L+LAG
Sbjct: 246 KKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGT 305
Query: 384 DTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLN 443
DT+A+TLEWALS +LN+P+VL KAR EID IG DRL+ E D+S+L YLQ +++ETLRL
Sbjct: 306 DTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLY 365
Query: 444 PAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGV 503
PAAP+L+PH ASEDCK+ GYD+PR TI+L N WAIHRD +W+D SFKPER
Sbjct: 366 PAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKE---G 425
Query: 504 DSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQ 563
++ KL+PFGLGRRACPG G+A R++ LTL SLIQC EWE++ VDM+EG+G+TMPKA+
Sbjct: 426 EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAK 485
Query: 564 PLVAKCKPRP 572
PL A C+ RP
Sbjct: 486 PLEAMCRARP 487
BLAST of Moc02g12450 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 532.3 bits (1370), Expect = 4.8e-151
Identity = 261/488 (53.48%), Postives = 347/488 (71.11%), Query Frame = 0
Query: 90 LSLFSLFIFFHFLLRPYRR--NLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYGH--V 149
LS L I LL R NLPPSP + LP+IGHLHL+K PVHRT H +S + G+ +
Sbjct: 9 LSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPI 68
Query: 150 FSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRN 209
F LR G+RLV V+SS S +ECFTKND+VLANRP + K + YN T + + YG+HWRN
Sbjct: 69 FHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRN 128
Query: 210 LRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVR 269
LRRI+ +EIFS+ R++ F IR+DE++ L+ L DSL F VEL S+L + FN ++
Sbjct: 129 LRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIM 188
Query: 270 MIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIMKIG 329
M+AGK+YYG D ++K RE++ EI+A G+ NL D++P NW+ T +E + +G
Sbjct: 189 MVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKILG 248
Query: 330 ERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGIDTAA 389
R D +LQ+LVDE R +K+ G T+IDHLLS Q T+PEY++D IIKG+ L+LAG DT++
Sbjct: 249 NRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAGTDTSS 308
Query: 390 ITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLNPAAP 449
+TLEWA+S LLN+PE+L+KARAEID IG DRLV E D+ +L YLQ +++ETLRL PA P
Sbjct: 309 VTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAVP 368
Query: 450 LLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGVDSYK 509
LL+PH +S++CK+ GYD+PR T++L N WA+HRD +WE+ FKPER ++ K
Sbjct: 369 LLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKE---GEARK 428
Query: 510 LLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQPLVA 569
L+PFG+GRRACPG + +R+V L L LIQ FEWERV + LVDMTEGEG+TMPKA PL A
Sbjct: 429 LMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPLRA 488
Query: 570 KCKPRPIL 574
CK R I+
Sbjct: 489 MCKARAIV 491
BLAST of Moc02g12450 vs. TAIR 10
Match:
AT3G28740.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 525.8 bits (1353), Expect = 4.5e-149
Identity = 267/509 (52.46%), Postives = 354/509 (69.55%), Query Frame = 0
Query: 74 ISNTKRRMDILLLSISLSLFSLFIFFHFLL--RPYRRNLPPSPFFRLPLIGHLHLVKHPV 133
+S+TK M+ + L +SL F +F+ L RP + NLPPSP P+IGHLHL+K P+
Sbjct: 1 MSSTKTIMETIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPL 60
Query: 134 HRTLHKLSH--NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAY 193
HR LS N +FSL GSRLV VVSS + +ECFTKND+VLANRP GK + Y
Sbjct: 61 HRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGY 120
Query: 194 NHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAV 253
N TT+ + YG+ WRNLRRI T+EIFS+ RLN F+ IR+DE++ L+ L +S F V
Sbjct: 121 NSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKV 180
Query: 254 ELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLW 313
E+ + + T N ++RM+AGK++YG+ ++ ++K R+++ E++ +GG N D+ P+
Sbjct: 181 EMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPIL 240
Query: 314 NWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQII 373
++ T YEK + K+ R DE LQ LV+E R +K GNTMIDHLLSLQ TQP+Y++D II
Sbjct: 241 RYV--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVII 300
Query: 374 KGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSY 433
KG+ +++LAG DT+A TLEWA+S LLN+PEVL+KA+ EID IG DRLV E D+ L Y
Sbjct: 301 KGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPY 360
Query: 434 LQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSF 493
LQ +++ETLRL P AP+L+PH ASEDC ++GYD+PR TIIL+NAWAIHRD +WE+ F
Sbjct: 361 LQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKF 420
Query: 494 KPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDM 553
KPER G D KL+PFG+GRR+CPG G+AQR+V L L SL+QCFEWERV +DM
Sbjct: 421 KPERFEKK--GEDK-KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDM 480
Query: 554 TEGE-GLTMPKAQPLVAKCKPRPILKAIL 578
E E G TM KA L A CK RPI+ +L
Sbjct: 481 RESEKGTTMRKATSLQAMCKARPIVHKVL 504
BLAST of Moc02g12450 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 520.8 bits (1340), Expect = 1.4e-147
Identity = 268/498 (53.82%), Postives = 350/498 (70.28%), Query Frame = 0
Query: 83 ILLLSISLSLFSLFIFFHFLLRPYRR-NLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHN 142
IL+LS LSLF+L IF L R R+ NLPPSP LP+IGHLHL+K P+HRT LS +
Sbjct: 6 ILILSF-LSLFTLAIF--LLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKS 65
Query: 143 YGH--VFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPY 202
G+ VF LR G+RLV V+SS S +ECFTKND+VLANRP K L YN T L + Y
Sbjct: 66 IGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASY 125
Query: 203 GEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHT 262
G+HWRNLRRI+ +EIFST RLN FL IR+DE++ L+ L DSL F VE+ ++L +
Sbjct: 126 GDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLA 185
Query: 263 FNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEK 322
N +RM+AGK+Y+GED D +K + +V E + + G N D++ + W+ + YEK
Sbjct: 186 SNTTIRMLAGKRYFGEDNDD---AKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEK 245
Query: 323 RIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLA 382
RI +G R D LQ+LVDE R +K+ G TMIDHLL+LQ+ QP+Y++D IIKG+ L++A
Sbjct: 246 RIKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIA 305
Query: 383 GIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLR 442
G DT+++TLEWA+S LLN+PE+LKKAR EID +G DRLV E D+ +LSYLQ ++ ETLR
Sbjct: 306 GTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLR 365
Query: 443 LNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG 502
+ PA PLL+PH +SEDCK+ GYDIP T++L NAWA+HRD +WED FKPER
Sbjct: 366 MYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKE-- 425
Query: 503 GVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPK 562
++ KL+ FG+GRRACPG G+A R++ L SL+QCFEWERV VDMTE +G T+PK
Sbjct: 426 -GEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPK 485
Query: 563 AQPLVAKCKPRPILKAIL 578
A PL A CK R I+ ++
Sbjct: 486 AIPLRAMCKARSIVDKLI 492
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
W8JMU7 | 2.8e-164 | 57.34 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q9FG65 | 6.1e-151 | 55.29 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
P93147 | 1.0e-150 | 53.60 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q6WNQ8 | 7.5e-149 | 54.02 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q9LHA1 | 6.3e-148 | 52.46 | Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1D5S6 | 0.0e+00 | 100.00 | isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... | [more] |
A0A6J1D5S1 | 6.4e-228 | 78.27 | cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV... | [more] |
A0A6J1G5P7 | 3.5e-218 | 73.95 | cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1 | [more] |
A0A6J1L2W8 | 1.3e-217 | 73.55 | cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1 | [more] |
A0A6J1D797 | 5.1e-217 | 73.29 | isoflavone 3'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017936 PE... | [more] |