Moc02g12450 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g12450
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioncytochrome P450 81E8-like
Locationchr2: 9043788 .. 9045640 (+)
RNA-Seq ExpressionMoc02g12450
SyntenyMoc02g12450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGCACCTAGTGGAAAAACATGTAAATTATTTTTAAAAAAATATCCAACATTATATTTTTTAATGATTAGTCAACTTTTGAGCTGTGAACATAGCTCAGATGGGAGTCCTTTTCCCAGCTTTCCATTCAAACTTGGCTGCACCCAAAATTTCCTCTCCCATTCTTTCCCCTTGGCAGAACCCATATTTACACATGCATCAAATTCAACTTCTCTGATCAGCAACACCAAAAGGAGAATGGACATTCTTTTGCTTTCCATTTCTCTCTCTCTCTTCTCTCTCTTCATCTTCTTCCACTTTCTGCTGCGACCCTATCGTCGAAACCTCCCACCGAGCCCATTCTTTCGCCTTCCCCTAATCGGCCATCTCCATCTCGTCAAACATCCTGTCCATCGAACGCTTCACAAGCTCTCGCACAACTATGGCCATGTCTTCTCTCTCCGATACGGCTCACGCCTTGTCGTAGTTGTGTCATCTCCTTCTGCTGTGCAAGAATGCTTTACGAAGAACGATATCGTTCTCGCTAATCGGCCGTTGTTGAAGAGCGGCAAGTTTCTAGCATACAACCACACCACCTTGGCGGTCTCCCCTTACGGCGAACATTGGCGTAACCTTCGTCGAATCAGCACTCTCGAGATTTTCTCGACGAGTCGCCTTAATCTCTTCTTGGGAATTCGAGAGGACGAAGTCAAAAACTTGTTGCGTAAATTGCGCGGTGACTCGCTGCAAGAATTTAGAGCGGTCGAGCTGGACTCGATGTTGTTAGATCATACGTTCAACATTGTTGTGAGAATGATTGCGGGAAAGAAGTATTATGGAGAGGACTTGAGGGATGAGGGGCAATCGAAGAAATTTAGGGAGATGGTGAAAGAAATTCTTGCAAATGGTGGGACAGCAAATTTGGGAGACTTTGTACCCTTATGGAATTGGATTGATCCAACTGGATATGAGAAGAGGATAATGAAGATTGGAGAGAGATCTGATGAACTCCTTCAAGAATTGGTTGATGAGATTAGGAATCAAAAGGATGGAGGAAACACCATGATTGACCATTTGCTTAGCTTGCAAAACACCCAACCTGAATATTTTAGTGACCAAATTATCAAAGGTCTTGCACAAGTATGTTTCTCTCTCTAGTTAGGGAGAATATCGTCTTTATCAATATCTCTATCATATTTTTGTTCTAAAAAATTATCAATTTGATCTCGCAGATTCTATTATTAGCGGGAATCGACACGGCAGCTATAACGTTAGAATGGGCCCTATCTCAACTACTCAACAATCCTGAGGTGTTGAAGAAAGCAAGAGCTGAGATAGATCATTTTATTGGCGAAGATCGTCTGGTTAGCGAAGGTGATTTGTCGAGTTTGAGTTACTTGCAAGGGGTAATCACTGAGACCCTGCGATTGAACCCAGCAGCTCCTTTGCTCGTGCCACACTGCGCCTCTGAAGACTGCAAAATAGAAGGCTATGATATACCGCGTGATACTATTATATTGATCAATGCTTGGGCTATTCATAGAGACTCGAATATATGGGAAGATGCCACTAGTTTCAAGCCTGAAAGACATGCAAACACTATGGGAGGAGTTGATTCATACAAATTGTTACCATTTGGGCTGGGGAGGAGAGCCTGCCCAGGAATGGGAATGGCACAGCGTGTGGTTGGCTTGACTTTGGCATCATTGATTCAATGTTTTGAATGGGAAAGAGTGAACAGTTCATTGGTTGACATGACTGAAGGTGAAGGGCTCACTATGCCCAAAGCTCAACCATTAGTAGCCAAGTGTAAACCACGTCCAATCTTGAAAGCTATTCTTGGTGTTGGTGGCACAATTCATAATATTTGA

mRNA sequence

ATGGATGCACCTAGTGGAAAAACATGTAAATTATTTTTAAAAAAATATCCAACATTATATTTTTTAATGATTAGTCAACTTTTGAGCTGTGAACATAGCTCAGATGGGAGTCCTTTTCCCAGCTTTCCATTCAAACTTGGCTGCACCCAAAATTTCCTCTCCCATTCTTTCCCCTTGGCAGAACCCATATTTACACATGCATCAAATTCAACTTCTCTGATCAGCAACACCAAAAGGAGAATGGACATTCTTTTGCTTTCCATTTCTCTCTCTCTCTTCTCTCTCTTCATCTTCTTCCACTTTCTGCTGCGACCCTATCGTCGAAACCTCCCACCGAGCCCATTCTTTCGCCTTCCCCTAATCGGCCATCTCCATCTCGTCAAACATCCTGTCCATCGAACGCTTCACAAGCTCTCGCACAACTATGGCCATGTCTTCTCTCTCCGATACGGCTCACGCCTTGTCGTAGTTGTGTCATCTCCTTCTGCTGTGCAAGAATGCTTTACGAAGAACGATATCGTTCTCGCTAATCGGCCGTTGTTGAAGAGCGGCAAGTTTCTAGCATACAACCACACCACCTTGGCGGTCTCCCCTTACGGCGAACATTGGCGTAACCTTCGTCGAATCAGCACTCTCGAGATTTTCTCGACGAGTCGCCTTAATCTCTTCTTGGGAATTCGAGAGGACGAAGTCAAAAACTTGTTGCGTAAATTGCGCGGTGACTCGCTGCAAGAATTTAGAGCGGTCGAGCTGGACTCGATGTTGTTAGATCATACGTTCAACATTGTTGTGAGAATGATTGCGGGAAAGAAGTATTATGGAGAGGACTTGAGGGATGAGGGGCAATCGAAGAAATTTAGGGAGATGGTGAAAGAAATTCTTGCAAATGGTGGGACAGCAAATTTGGGAGACTTTGTACCCTTATGGAATTGGATTGATCCAACTGGATATGAGAAGAGGATAATGAAGATTGGAGAGAGATCTGATGAACTCCTTCAAGAATTGGTTGATGAGATTAGGAATCAAAAGGATGGAGGAAACACCATGATTGACCATTTGCTTAGCTTGCAAAACACCCAACCTGAATATTTTAGTGACCAAATTATCAAAGGTCTTGCACAAATTCTATTATTAGCGGGAATCGACACGGCAGCTATAACGTTAGAATGGGCCCTATCTCAACTACTCAACAATCCTGAGGTGTTGAAGAAAGCAAGAGCTGAGATAGATCATTTTATTGGCGAAGATCGTCTGGTTAGCGAAGGTGATTTGTCGAGTTTGAGTTACTTGCAAGGGGTAATCACTGAGACCCTGCGATTGAACCCAGCAGCTCCTTTGCTCGTGCCACACTGCGCCTCTGAAGACTGCAAAATAGAAGGCTATGATATACCGCGTGATACTATTATATTGATCAATGCTTGGGCTATTCATAGAGACTCGAATATATGGGAAGATGCCACTAGTTTCAAGCCTGAAAGACATGCAAACACTATGGGAGGAGTTGATTCATACAAATTGTTACCATTTGGGCTGGGGAGGAGAGCCTGCCCAGGAATGGGAATGGCACAGCGTGTGGTTGGCTTGACTTTGGCATCATTGATTCAATGTTTTGAATGGGAAAGAGTGAACAGTTCATTGGTTGACATGACTGAAGGTGAAGGGCTCACTATGCCCAAAGCTCAACCATTAGTAGCCAAGTGTAAACCACGTCCAATCTTGAAAGCTATTCTTGGTGTTGGTGGCACAATTCATAATATTTGA

Coding sequence (CDS)

ATGGATGCACCTAGTGGAAAAACATGTAAATTATTTTTAAAAAAATATCCAACATTATATTTTTTAATGATTAGTCAACTTTTGAGCTGTGAACATAGCTCAGATGGGAGTCCTTTTCCCAGCTTTCCATTCAAACTTGGCTGCACCCAAAATTTCCTCTCCCATTCTTTCCCCTTGGCAGAACCCATATTTACACATGCATCAAATTCAACTTCTCTGATCAGCAACACCAAAAGGAGAATGGACATTCTTTTGCTTTCCATTTCTCTCTCTCTCTTCTCTCTCTTCATCTTCTTCCACTTTCTGCTGCGACCCTATCGTCGAAACCTCCCACCGAGCCCATTCTTTCGCCTTCCCCTAATCGGCCATCTCCATCTCGTCAAACATCCTGTCCATCGAACGCTTCACAAGCTCTCGCACAACTATGGCCATGTCTTCTCTCTCCGATACGGCTCACGCCTTGTCGTAGTTGTGTCATCTCCTTCTGCTGTGCAAGAATGCTTTACGAAGAACGATATCGTTCTCGCTAATCGGCCGTTGTTGAAGAGCGGCAAGTTTCTAGCATACAACCACACCACCTTGGCGGTCTCCCCTTACGGCGAACATTGGCGTAACCTTCGTCGAATCAGCACTCTCGAGATTTTCTCGACGAGTCGCCTTAATCTCTTCTTGGGAATTCGAGAGGACGAAGTCAAAAACTTGTTGCGTAAATTGCGCGGTGACTCGCTGCAAGAATTTAGAGCGGTCGAGCTGGACTCGATGTTGTTAGATCATACGTTCAACATTGTTGTGAGAATGATTGCGGGAAAGAAGTATTATGGAGAGGACTTGAGGGATGAGGGGCAATCGAAGAAATTTAGGGAGATGGTGAAAGAAATTCTTGCAAATGGTGGGACAGCAAATTTGGGAGACTTTGTACCCTTATGGAATTGGATTGATCCAACTGGATATGAGAAGAGGATAATGAAGATTGGAGAGAGATCTGATGAACTCCTTCAAGAATTGGTTGATGAGATTAGGAATCAAAAGGATGGAGGAAACACCATGATTGACCATTTGCTTAGCTTGCAAAACACCCAACCTGAATATTTTAGTGACCAAATTATCAAAGGTCTTGCACAAATTCTATTATTAGCGGGAATCGACACGGCAGCTATAACGTTAGAATGGGCCCTATCTCAACTACTCAACAATCCTGAGGTGTTGAAGAAAGCAAGAGCTGAGATAGATCATTTTATTGGCGAAGATCGTCTGGTTAGCGAAGGTGATTTGTCGAGTTTGAGTTACTTGCAAGGGGTAATCACTGAGACCCTGCGATTGAACCCAGCAGCTCCTTTGCTCGTGCCACACTGCGCCTCTGAAGACTGCAAAATAGAAGGCTATGATATACCGCGTGATACTATTATATTGATCAATGCTTGGGCTATTCATAGAGACTCGAATATATGGGAAGATGCCACTAGTTTCAAGCCTGAAAGACATGCAAACACTATGGGAGGAGTTGATTCATACAAATTGTTACCATTTGGGCTGGGGAGGAGAGCCTGCCCAGGAATGGGAATGGCACAGCGTGTGGTTGGCTTGACTTTGGCATCATTGATTCAATGTTTTGAATGGGAAAGAGTGAACAGTTCATTGGTTGACATGACTGAAGGTGAAGGGCTCACTATGCCCAAAGCTCAACCATTAGTAGCCAAGTGTAAACCACGTCCAATCTTGAAAGCTATTCTTGGTGTTGGTGGCACAATTCATAATATTTGA

Protein sequence

MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLAEPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI
Homology
BLAST of Moc02g12450 vs. NCBI nr
Match: XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0

Query: 1   MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA 60
           MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA
Sbjct: 1   MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA 60

Query: 61  EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL 120
           EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL
Sbjct: 61  EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL 120

Query: 121 IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL 180
           IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL
Sbjct: 121 IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL 180

Query: 181 LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG 240
           LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG
Sbjct: 181 LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG 240

Query: 241 DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA 300
           DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA
Sbjct: 241 DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA 300

Query: 301 NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ 360
           NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ
Sbjct: 301 NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ 360

Query: 361 PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS 420
           PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS
Sbjct: 361 PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS 420

Query: 421 EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS 480
           EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS
Sbjct: 421 EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS 480

Query: 481 NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE 540
           NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE
Sbjct: 481 NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE 540

Query: 541 RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI 587
           RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI
Sbjct: 541 RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI 586

BLAST of Moc02g12450 vs. NCBI nr
Match: XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])

HSP 1 Score: 800.0 bits (2065), Expect = 1.3e-227
Identity = 389/497 (78.27%), Postives = 445/497 (89.54%), Query Frame = 0

Query: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
           MDIL LSISLSLFSL + F FL R  R+NLPPSP F LP++GHLHLVKHPVH+T H+LSH
Sbjct: 1   MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60

Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
            YGHVFSLR+GSR+VVVVSSPSAVQECFTKNDIVLANRPLLKSGK LAYNHTT+AV+PYG
Sbjct: 61  KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120

Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
           EHWRNLRRI TLEIFST+RLN+FLGIREDEV+NL+ KL  +S +EF AVEL+ ML D TF
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180

Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
           NIV+RM+AGKKYY     +E QSKKFREMV ++LA+ G  N GDF+P+WNWIDPTG+EKR
Sbjct: 181 NIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 240

Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
           I+K+G+RSDELLQEL+DEIRNQ+ G NTMIDHLL LQ  +PEY+SDQIIKGL Q++LLAG
Sbjct: 241 IIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAG 300

Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
           IDTAA+TLEWALS+LLNNPEVL+KA+AEID F+G++RLV+E DLSSL YLQG+I ETLRL
Sbjct: 301 IDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRL 360

Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
           NPAAPLL+PHCAS DCKI GYD+PRDTIILIN WAIHRD N+WEDAT+FKPERH NT+ G
Sbjct: 361 NPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTI-G 420

Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
           VDSYKLLPFG GRR+CPG+GMAQRVVGLTLASLIQ FEWER+++SLVDMTEG+GLTMPKA
Sbjct: 421 VDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKA 480

Query: 561 QPLVAKCKPRPILKAIL 578
           Q LVAKCKPRPI+K IL
Sbjct: 481 QHLVAKCKPRPIMKLIL 491

BLAST of Moc02g12450 vs. NCBI nr
Match: XP_022947035.1 (cytochrome P450 81F3 [Cucurbita moschata])

HSP 1 Score: 767.7 bits (1981), Expect = 7.3e-218
Identity = 369/499 (73.95%), Postives = 433/499 (86.77%), Query Frame = 0

Query: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
           MDILLLSI L L SL   F+FL  P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS 
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60

Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
            +GHV SLR+GSRLVVV+SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLAVSPYG
Sbjct: 61  KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120

Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
           EHWRNLRRI +LEIFST+RLN+F+GIRE+EVK LLRKL GD  +EFR VEL+ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180

Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
           N+V+ M+ G+++Y     DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240

Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
           IM +  R DELLQEL+DE R+ KDGGNTMI HLL LQ  +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300

Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
           IDT+A+TLEWALS LLNNP+VLKKARAEID  IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRL 360

Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
           NPAAPLLVPHCA +DCKIEGYD+PRDTI+LIN WAIHRD N+WED T+FKPERH +T  G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDT-SG 420

Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
            DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480

Query: 561 QPLVAKCKPRPILKAILGV 580
           QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493

BLAST of Moc02g12450 vs. NCBI nr
Match: XP_023007415.1 (cytochrome P450 81F3-like [Cucurbita maxima])

HSP 1 Score: 765.8 bits (1976), Expect = 2.8e-217
Identity = 367/499 (73.55%), Postives = 435/499 (87.17%), Query Frame = 0

Query: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
           MDILLLSI L L SL   F+FL  P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS 
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60

Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
            +GHV SLR+GSRLVV++SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLA+SPYG
Sbjct: 61  KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120

Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
           EHWRNLRRI +LEIFSTSRLNLFLGIR++EVK LLRKL GD L+EFR VE++ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180

Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
           N+V+ M+ G+++Y     DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240

Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
           IM +  R DELLQEL+DE R+ KDGGN+MI HLL LQ  +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300

Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
           IDT+A+TLEWALS LLNNP+VLKKARAEID  IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRL 360

Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
           NPAAPLLVPHCA +DCKIEGYD+PRDTI+LINAWAIHRD ++WED T+FKPERH ++  G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDS-SG 420

Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
            DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480

Query: 561 QPLVAKCKPRPILKAILGV 580
           QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493

BLAST of Moc02g12450 vs. NCBI nr
Match: XP_023532689.1 (cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.1 cytochrome P450 81F3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 765.8 bits (1976), Expect = 2.8e-217
Identity = 368/499 (73.75%), Postives = 434/499 (86.97%), Query Frame = 0

Query: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
           MDILLLSI L L SL   F+FL  P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS 
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHTLSQ 60

Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
            +GHV SLR+GSRLVVV+SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLA+SPYG
Sbjct: 61  KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120

Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
           EHWRNLRRI +LEIFST+RLN+F+GIRE+EVK LLRKL G  L+E R VEL+ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGGWLEEGREVELEPMLSDLTF 180

Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
           N+V+ M+ G+++Y     DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240

Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
           IM +  R DE+LQEL+DE R+ KDGGNTMI HLL LQ  +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDEVLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300

Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
           IDT+A+TLEWALSQLLNNP+VLKKARAEID  IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSQLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRL 360

Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
           NPAAPLLVPHCA +DCKIEGYD+PRDTI+LINAWAIHRD N+WED T+FKPERH +T  G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPNLWEDPTAFKPERHGDT-SG 420

Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
            DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480

Query: 561 QPLVAKCKPRPILKAILGV 580
           QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493

BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.8e-164
Identity = 285/497 (57.34%), Postives = 369/497 (74.25%), Query Frame = 0

Query: 83  ILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNY 142
           ++LLSISL LF         +   RRNLPPSP   LP+IGHLHL+   +HR+L+ LS  Y
Sbjct: 13  VVLLSISLKLFP--------VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 72

Query: 143 GHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEH 202
           G VFSL+ G+RLV+VVSSP+A +ECFTKNDIV ANRPL   GK++ YN+TT+  SPYGEH
Sbjct: 73  GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 132

Query: 203 WRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNI 262
           WRNLRR++ +EIFS   LN FL IREDEVK LL  L   S Q+F  VE+ S L + +FN+
Sbjct: 133 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNV 192

Query: 263 VVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIM 322
            +RM+AGK+Y+G+D+ D  ++K FR ++ E+  + G +N GDFVP   WID   YEK++ 
Sbjct: 193 TMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVS 252

Query: 323 KIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGID 382
           KI +  D  LQ L+ E R  K+   TMIDHLLSLQ +QPEY++DQIIKG+  +LLLAG D
Sbjct: 253 KISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTD 312

Query: 383 TAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLNP 442
           T+A+T+EWA+S LLN+PE L+KAR EI+  +G +RL+ E DL  L+YL  +I+ET RL P
Sbjct: 313 TSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCP 372

Query: 443 AAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGV- 502
           AAP+LVPH +S+DCK++GYD+P+ TI+L+NAWAIHRD   W++ T FKPERH    GGV 
Sbjct: 373 AAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH----GGVE 432

Query: 503 -DSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 562
            +  KL+PFG+GRR+CPG G+AQRVVGLTL +LIQCFEW+R+  + +DM EG GLTMPKA
Sbjct: 433 LEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKA 492

Query: 563 QPLVAKCKPRPILKAIL 578
           QPL A CKPR IL  ++
Sbjct: 493 QPLEALCKPRNILHKVV 495

BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 6.1e-151
Identity = 277/501 (55.29%), Postives = 357/501 (71.26%), Query Frame = 0

Query: 83  ILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNY 142
           ++L SI  SL  L I F F L+P ++NLPPSP   LP+IGHL L+K P+HRTL   S   
Sbjct: 8   VVLYSI-FSLIFLIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67

Query: 143 GH-----VFSLRYGSRLVVVVSSPS-AVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAV 202
            H     V SLR GSRLV VVSS   A +ECF KND+VLANRP +  GK + YN+T +  
Sbjct: 68  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127

Query: 203 SPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLL 262
           +PYG+HWRNLRR+ T+EIFST RLN FL +R DEV+ L+ +L   +  +   VEL  ML+
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 187

Query: 263 DHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTG 322
           D TFN ++RM+ GK+YYGE+  DE ++K+ R++V ++ AN  + N  D+VP+      + 
Sbjct: 188 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SS 247

Query: 323 YEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQIL 382
           YE R+ K+GE +D+ LQ L+D+ R Q++ G TMIDHLL LQ +  EY++DQIIKG+  I+
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 307

Query: 383 LLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITE 442
           ++AG +T+A+TLEWALS LLN+P+V+ KAR EID+ +G DRL+ E DLS L YL+ ++ E
Sbjct: 308 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 367

Query: 443 TLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHAN 502
           TLRL+PA PLLVPH ASEDCKI  YD+PR T +L+NAWAIHRD N W+D  SFKPER   
Sbjct: 368 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 427

Query: 503 TMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLT 562
                ++ KLL FGLGRRACPG G+AQR+VGL L SLIQCFEWERV +  VDM EG G T
Sbjct: 428 E---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNT 487

Query: 563 MPKAQPLVAKCKPRPILKAIL 578
           +PKA PL A CK RP L  I+
Sbjct: 488 VPKAIPLKAICKARPFLHKII 501

BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 535.0 bits (1377), Expect = 1.0e-150
Identity = 268/500 (53.60%), Postives = 359/500 (71.80%), Query Frame = 0

Query: 81  MDIL-LLSISLSLFSLFIFFHFLLRPYR-RNLPPSPFFRLPLIGHLHLVKHPVHRTLHKL 140
           M+IL LLS S+   +LF  F+ ++R  + +NLPP P   LP+IG+LH +K P+HRT   L
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGL 60

Query: 141 SHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSP 200
           S  YGHVFSL +GSRLVVVVSS S  Q+CFTKND+VLANRP   SGK++ YN+TTL  + 
Sbjct: 61  SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120

Query: 201 YGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDH 260
           YGEHWRNLRRI+ L++ S  R+N F GIR DE + L+ +L  DS   F  +EL S L D 
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 180

Query: 261 TFNIVVRMIAGKKYYGE--DLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTG 320
           TFN ++RMI+GK+YYGE  D  D  ++ +FR+MV E+L   G  N  DF+PL  ++D   
Sbjct: 181 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 240

Query: 321 YEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQIL 380
            EKR+  I  ++D  L+ L++E R +K+  NTMIDHLL+LQ++QPEY++DQIIKGLA  +
Sbjct: 241 LEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 300

Query: 381 LLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITE 440
           LLAG D++A+TLEW++S LLN+PEVLKK + E+D  +G+DRLV E DL  L+YL+ VI E
Sbjct: 301 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 360

Query: 441 TLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHAN 500
           TLRL   APLL+PH  S++C I GY +P+DTI+LINAWAIHRD  +W +AT+FKPER   
Sbjct: 361 TLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERF-E 420

Query: 501 TMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLT 560
             G ++  KL+ FG+GRRACPG G+A R + +TLA LIQCF+W+ +N   +D+ E +G T
Sbjct: 421 KKGELE--KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFT 480

Query: 561 MPKAQPLVAKCKPRPILKAI 577
           + K  PL A CK RP++  +
Sbjct: 481 LTKLVPLKAMCKSRPVINKV 496

BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 7.5e-149
Identity = 269/498 (54.02%), Postives = 349/498 (70.08%), Query Frame = 0

Query: 81  MDILLLSISLSLFSLFIFFHFLLRPYR--RNLPPSPFFRLPLIGHLHLVKHPVHRTLHKL 140
           M    LS+ +SLF L I         R  +NLPP P   LP+IG+LH +K P+H T H L
Sbjct: 1   MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQC-LPIIGNLHQLKQPLHHTFHTL 60

Query: 141 SHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSP 200
           S  YG +FSL +GSRLVVVVSS +  QECFTKNDIVLANRP   +GK++ YN+TT+A SP
Sbjct: 61  SQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSP 120

Query: 201 YGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDH 260
           YG+HWRNLRRI ++EI S+ RLN FL IR DE+  L++KL   S   F  VEL  M  + 
Sbjct: 121 YGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEM 180

Query: 261 TFNIVVRMIAGKKYYGE--DLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTG 320
           TFN ++RM++GK+YYG   D+ D  +++ FR ++KE+++ GG  N+GDF+    W D  G
Sbjct: 181 TFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDG 240

Query: 321 YEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQIL 380
            EKR+ KI +R+D  LQ L+DE R  K   NTMIDHLL+ Q +QPEY++DQIIKGL  ++
Sbjct: 241 LEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 300

Query: 381 LLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITE 440
           LLAG DT+++T+EWA+S LLN+PE++KKA+ E+D  IG DR V E D+S L YLQ ++ E
Sbjct: 301 LLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYE 360

Query: 441 TLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHAN 500
           TLRL+ AAPLLVPH +SED  + GY+IP++TI+++NAW IHRD N+W D T FKPER   
Sbjct: 361 TLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK 420

Query: 501 TMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLT 560
             G V+  KLL FGLGRRACPG  ++QR  GLTL  LIQCFEW+R+    +DM E +G+T
Sbjct: 421 E-GEVN--KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGIT 480

Query: 561 MPKAQPLVAKCKPRPILK 575
             K   L A CK R  LK
Sbjct: 481 AGKKTSLNAMCKVRHPLK 494

BLAST of Moc02g12450 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 6.3e-148
Identity = 267/509 (52.46%), Postives = 354/509 (69.55%), Query Frame = 0

Query: 74  ISNTKRRMDILLLSISLSLFSLFIFFHFLL--RPYRRNLPPSPFFRLPLIGHLHLVKHPV 133
           +S+TK  M+ + L +SL  F +F+    L   RP + NLPPSP    P+IGHLHL+K P+
Sbjct: 1   MSSTKTIMETIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPL 60

Query: 134 HRTLHKLSH--NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAY 193
           HR    LS   N   +FSL  GSRLV VVSS +  +ECFTKND+VLANRP    GK + Y
Sbjct: 61  HRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGY 120

Query: 194 NHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAV 253
           N TT+  + YG+ WRNLRRI T+EIFS+ RLN F+ IR+DE++ L+  L  +S   F  V
Sbjct: 121 NSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKV 180

Query: 254 ELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLW 313
           E+  + +  T N ++RM+AGK++YG+   ++ ++K  R+++ E++ +GG  N  D+ P+ 
Sbjct: 181 EMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPIL 240

Query: 314 NWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQII 373
            ++  T YEK + K+  R DE LQ LV+E R +K  GNTMIDHLLSLQ TQP+Y++D II
Sbjct: 241 RYV--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVII 300

Query: 374 KGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSY 433
           KG+  +++LAG DT+A TLEWA+S LLN+PEVL+KA+ EID  IG DRLV E D+  L Y
Sbjct: 301 KGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPY 360

Query: 434 LQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSF 493
           LQ +++ETLRL P AP+L+PH ASEDC ++GYD+PR TIIL+NAWAIHRD  +WE+   F
Sbjct: 361 LQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKF 420

Query: 494 KPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDM 553
           KPER      G D  KL+PFG+GRR+CPG G+AQR+V L L SL+QCFEWERV    +DM
Sbjct: 421 KPERFEKK--GEDK-KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDM 480

Query: 554 TEGE-GLTMPKAQPLVAKCKPRPILKAIL 578
            E E G TM KA  L A CK RPI+  +L
Sbjct: 481 RESEKGTTMRKATSLQAMCKARPIVHKVL 504

BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0

Query: 1   MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA 60
           MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA
Sbjct: 1   MDAPSGKTCKLFLKKYPTLYFLMISQLLSCEHSSDGSPFPSFPFKLGCTQNFLSHSFPLA 60

Query: 61  EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL 120
           EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL
Sbjct: 61  EPIFTHASNSTSLISNTKRRMDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPL 120

Query: 121 IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL 180
           IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL
Sbjct: 121 IGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPL 180

Query: 181 LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG 240
           LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG
Sbjct: 181 LKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRG 240

Query: 241 DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA 300
           DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA
Sbjct: 241 DSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTA 300

Query: 301 NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ 360
           NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ
Sbjct: 301 NLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQ 360

Query: 361 PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS 420
           PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS
Sbjct: 361 PEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVS 420

Query: 421 EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS 480
           EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS
Sbjct: 421 EGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDS 480

Query: 481 NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE 540
           NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE
Sbjct: 481 NIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWE 540

Query: 541 RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI 587
           RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI
Sbjct: 541 RVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAILGVGGTIHNI 586

BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match: A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 6.4e-228
Identity = 389/497 (78.27%), Postives = 445/497 (89.54%), Query Frame = 0

Query: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
           MDIL LSISLSLFSL + F FL R  R+NLPPSP F LP++GHLHLVKHPVH+T H+LSH
Sbjct: 1   MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60

Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
            YGHVFSLR+GSR+VVVVSSPSAVQECFTKNDIVLANRPLLKSGK LAYNHTT+AV+PYG
Sbjct: 61  KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120

Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
           EHWRNLRRI TLEIFST+RLN+FLGIREDEV+NL+ KL  +S +EF AVEL+ ML D TF
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180

Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
           NIV+RM+AGKKYY     +E QSKKFREMV ++LA+ G  N GDF+P+WNWIDPTG+EKR
Sbjct: 181 NIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 240

Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
           I+K+G+RSDELLQEL+DEIRNQ+ G NTMIDHLL LQ  +PEY+SDQIIKGL Q++LLAG
Sbjct: 241 IIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAG 300

Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
           IDTAA+TLEWALS+LLNNPEVL+KA+AEID F+G++RLV+E DLSSL YLQG+I ETLRL
Sbjct: 301 IDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRL 360

Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
           NPAAPLL+PHCAS DCKI GYD+PRDTIILIN WAIHRD N+WEDAT+FKPERH NT+ G
Sbjct: 361 NPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTI-G 420

Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
           VDSYKLLPFG GRR+CPG+GMAQRVVGLTLASLIQ FEWER+++SLVDMTEG+GLTMPKA
Sbjct: 421 VDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKA 480

Query: 561 QPLVAKCKPRPILKAIL 578
           Q LVAKCKPRPI+K IL
Sbjct: 481 QHLVAKCKPRPIMKLIL 491

BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match: A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 3.5e-218
Identity = 369/499 (73.95%), Postives = 433/499 (86.77%), Query Frame = 0

Query: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
           MDILLLSI L L SL   F+FL  P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS 
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60

Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
            +GHV SLR+GSRLVVV+SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLAVSPYG
Sbjct: 61  KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120

Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
           EHWRNLRRI +LEIFST+RLN+F+GIRE+EVK LLRKL GD  +EFR VEL+ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180

Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
           N+V+ M+ G+++Y     DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240

Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
           IM +  R DELLQEL+DE R+ KDGGNTMI HLL LQ  +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300

Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
           IDT+A+TLEWALS LLNNP+VLKKARAEID  IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRL 360

Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
           NPAAPLLVPHCA +DCKIEGYD+PRDTI+LIN WAIHRD N+WED T+FKPERH +T  G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDT-SG 420

Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
            DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480

Query: 561 QPLVAKCKPRPILKAILGV 580
           QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493

BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match: A0A6J1L2W8 (cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 1.3e-217
Identity = 367/499 (73.55%), Postives = 435/499 (87.17%), Query Frame = 0

Query: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
           MDILLLSI L L SL   F+FL  P+R NLPP+P F LPLIGH HL+KHP+HR+LH LS 
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60

Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
            +GHV SLR+GSRLVV++SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLA+SPYG
Sbjct: 61  KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120

Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
           EHWRNLRRI +LEIFSTSRLNLFLGIR++EVK LLRKL GD L+EFR VE++ ML D TF
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180

Query: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
           N+V+ M+ G+++Y     DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KR
Sbjct: 181 NVVMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 240

Query: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
           IM +  R DELLQEL+DE R+ KDGGN+MI HLL LQ  +PEY+SD IIKGL Q++ LAG
Sbjct: 241 IMNLARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 300

Query: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
           IDT+A+TLEWALS LLNNP+VLKKARAEID  IG++RLV+E DL +L YLQG+I+ETLRL
Sbjct: 301 IDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRL 360

Query: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
           NPAAPLLVPHCA +DCKIEGYD+PRDTI+LINAWAIHRD ++WED T+FKPERH ++  G
Sbjct: 361 NPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDS-SG 420

Query: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
            DSYKLLPFGLGRRACPG+GMAQRVVGL LASLIQ FEWERV+SSLVDM+EG+GLTMPKA
Sbjct: 421 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480

Query: 561 QPLVAKCKPRPILKAILGV 580
           QPLVAKCKPRPI+KAI+ V
Sbjct: 481 QPLVAKCKPRPIMKAIMNV 493

BLAST of Moc02g12450 vs. ExPASy TrEMBL
Match: A0A6J1D797 (isoflavone 3'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017936 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 5.1e-217
Identity = 365/498 (73.29%), Postives = 434/498 (87.15%), Query Frame = 0

Query: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
           M+ LLLSI  SLFSL I   FL RP R NLPPSP F LPLIGH+HLVKHPVHRT HK+S 
Sbjct: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60

Query: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
            +G +FSLR+GSRL+VVVSS SAVQECFTKNDIVLANRP++KSGK+LAYN+TTL  SPYG
Sbjct: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120

Query: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
           EHWRNLRRIS LE+ STSRLN F+GIREDE+K L+ +L GDSL+EF  VE++ + LD TF
Sbjct: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180

Query: 261 NIVVRMIAGKKYYGEDL-RDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEK 320
           +IV+RM+AGKKYYG+D+  D  QS+KFRE+V +ILA+  + N GDF+P+WN IDP+G EK
Sbjct: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240

Query: 321 RIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLA 380
           RIMK+G+R+DEL Q L+DEIRNQ +GGNTMIDHLL LQNTQPEY+SDQ+IKG+ Q ++LA
Sbjct: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300

Query: 381 GIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLR 440
           GIDT+A+T+EWALS LLNNP+VLKKAR EID+ IG++R+V+E DLSSLSYLQG+I+ETLR
Sbjct: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360

Query: 441 LNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG 500
           L P  P+L+PHCASEDCK+EGYDIPRDTI+L+NAWAIHRD N+WEDA SFKPERH N + 
Sbjct: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAI- 420

Query: 501 GVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPK 560
             ++Y+LLPFG+GRRACPGMGMAQRVVGLTLASLIQCFEW+RV+SSLVDMTEGEG TMPK
Sbjct: 421 RPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPK 480

Query: 561 AQPLVAKCKPRPILKAIL 578
           AQPL+AKCKP PI+K IL
Sbjct: 481 AQPLIAKCKPHPIMKVIL 497

BLAST of Moc02g12450 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 535.8 bits (1379), Expect = 4.3e-152
Identity = 277/501 (55.29%), Postives = 357/501 (71.26%), Query Frame = 0

Query: 83  ILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNY 142
           ++L SI  SL  L I F F L+P ++NLPPSP   LP+IGHL L+K P+HRTL   S   
Sbjct: 8   VVLYSI-FSLIFLIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67

Query: 143 GH-----VFSLRYGSRLVVVVSSPS-AVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAV 202
            H     V SLR GSRLV VVSS   A +ECF KND+VLANRP +  GK + YN+T +  
Sbjct: 68  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127

Query: 203 SPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLL 262
           +PYG+HWRNLRR+ T+EIFST RLN FL +R DEV+ L+ +L   +  +   VEL  ML+
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 187

Query: 263 DHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTG 322
           D TFN ++RM+ GK+YYGE+  DE ++K+ R++V ++ AN  + N  D+VP+      + 
Sbjct: 188 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SS 247

Query: 323 YEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQIL 382
           YE R+ K+GE +D+ LQ L+D+ R Q++ G TMIDHLL LQ +  EY++DQIIKG+  I+
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 307

Query: 383 LLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITE 442
           ++AG +T+A+TLEWALS LLN+P+V+ KAR EID+ +G DRL+ E DLS L YL+ ++ E
Sbjct: 308 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 367

Query: 443 TLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHAN 502
           TLRL+PA PLLVPH ASEDCKI  YD+PR T +L+NAWAIHRD N W+D  SFKPER   
Sbjct: 368 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 427

Query: 503 TMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLT 562
                ++ KLL FGLGRRACPG G+AQR+VGL L SLIQCFEWERV +  VDM EG G T
Sbjct: 428 E---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNT 487

Query: 563 MPKAQPLVAKCKPRPILKAIL 578
           +PKA PL A CK RP L  I+
Sbjct: 488 VPKAIPLKAICKARPFLHKII 501

BLAST of Moc02g12450 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 533.5 bits (1373), Expect = 2.2e-151
Identity = 267/490 (54.49%), Postives = 355/490 (72.45%), Query Frame = 0

Query: 84  LLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH--N 143
           L+ SI   + SL      L R  + NLPPSP + LP+IGHL L+K P+HRT   LS   N
Sbjct: 6   LIFSILFVVLSLIYLIGKLKR--KPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 65

Query: 144 YGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGE 203
              +FSLR G+RLV V SS S  +ECFTKND+VLANRP     K +AY++TT+  + YG+
Sbjct: 66  NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 125

Query: 204 HWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFN 263
           HWRNLRRI ++EIFS  RLN FL IR+DE++ L+ +L  +  QEF  V++ SML D TFN
Sbjct: 126 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFN 185

Query: 264 IVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRI 323
            ++RM+AGK+YYG+ + D+ ++K+ R+++ +++A  G  N  D++P+   +  + YE R+
Sbjct: 186 NILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRV 245

Query: 324 MKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGI 383
            K+  R DE LQ LVDE R  K+ GNTMIDHLL+LQ +QP+YF+D+IIKG    L+LAG 
Sbjct: 246 KKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGT 305

Query: 384 DTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLN 443
           DT+A+TLEWALS +LN+P+VL KAR EID  IG DRL+ E D+S+L YLQ +++ETLRL 
Sbjct: 306 DTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLY 365

Query: 444 PAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGV 503
           PAAP+L+PH ASEDCK+ GYD+PR TI+L N WAIHRD  +W+D  SFKPER        
Sbjct: 366 PAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKE---G 425

Query: 504 DSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQ 563
           ++ KL+PFGLGRRACPG G+A R++ LTL SLIQC EWE++    VDM+EG+G+TMPKA+
Sbjct: 426 EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAK 485

Query: 564 PLVAKCKPRP 572
           PL A C+ RP
Sbjct: 486 PLEAMCRARP 487

BLAST of Moc02g12450 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 532.3 bits (1370), Expect = 4.8e-151
Identity = 261/488 (53.48%), Postives = 347/488 (71.11%), Query Frame = 0

Query: 90  LSLFSLFIFFHFLLRPYRR--NLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYGH--V 149
           LS   L I    LL    R  NLPPSP + LP+IGHLHL+K PVHRT H +S + G+  +
Sbjct: 9   LSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPI 68

Query: 150 FSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRN 209
           F LR G+RLV V+SS S  +ECFTKND+VLANRP +   K + YN T +  + YG+HWRN
Sbjct: 69  FHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRN 128

Query: 210 LRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVR 269
           LRRI+ +EIFS+ R++ F  IR+DE++ L+  L  DSL  F  VEL S+L +  FN ++ 
Sbjct: 129 LRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIM 188

Query: 270 MIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIMKIG 329
           M+AGK+YYG    D  ++K  RE++ EI+A  G+ NL D++P  NW+  T +E +   +G
Sbjct: 189 MVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKILG 248

Query: 330 ERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGIDTAA 389
            R D +LQ+LVDE R +K+ G T+IDHLLS Q T+PEY++D IIKG+   L+LAG DT++
Sbjct: 249 NRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAGTDTSS 308

Query: 390 ITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLNPAAP 449
           +TLEWA+S LLN+PE+L+KARAEID  IG DRLV E D+ +L YLQ +++ETLRL PA P
Sbjct: 309 VTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAVP 368

Query: 450 LLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGVDSYK 509
           LL+PH +S++CK+ GYD+PR T++L N WA+HRD  +WE+   FKPER        ++ K
Sbjct: 369 LLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKE---GEARK 428

Query: 510 LLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQPLVA 569
           L+PFG+GRRACPG  + +R+V L L  LIQ FEWERV + LVDMTEGEG+TMPKA PL A
Sbjct: 429 LMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPLRA 488

Query: 570 KCKPRPIL 574
            CK R I+
Sbjct: 489 MCKARAIV 491

BLAST of Moc02g12450 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 525.8 bits (1353), Expect = 4.5e-149
Identity = 267/509 (52.46%), Postives = 354/509 (69.55%), Query Frame = 0

Query: 74  ISNTKRRMDILLLSISLSLFSLFIFFHFLL--RPYRRNLPPSPFFRLPLIGHLHLVKHPV 133
           +S+TK  M+ + L +SL  F +F+    L   RP + NLPPSP    P+IGHLHL+K P+
Sbjct: 1   MSSTKTIMETIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPL 60

Query: 134 HRTLHKLSH--NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAY 193
           HR    LS   N   +FSL  GSRLV VVSS +  +ECFTKND+VLANRP    GK + Y
Sbjct: 61  HRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGY 120

Query: 194 NHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAV 253
           N TT+  + YG+ WRNLRRI T+EIFS+ RLN F+ IR+DE++ L+  L  +S   F  V
Sbjct: 121 NSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKV 180

Query: 254 ELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLW 313
           E+  + +  T N ++RM+AGK++YG+   ++ ++K  R+++ E++ +GG  N  D+ P+ 
Sbjct: 181 EMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPIL 240

Query: 314 NWIDPTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQII 373
            ++  T YEK + K+  R DE LQ LV+E R +K  GNTMIDHLLSLQ TQP+Y++D II
Sbjct: 241 RYV--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVII 300

Query: 374 KGLAQILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSY 433
           KG+  +++LAG DT+A TLEWA+S LLN+PEVL+KA+ EID  IG DRLV E D+  L Y
Sbjct: 301 KGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPY 360

Query: 434 LQGVITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSF 493
           LQ +++ETLRL P AP+L+PH ASEDC ++GYD+PR TIIL+NAWAIHRD  +WE+   F
Sbjct: 361 LQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKF 420

Query: 494 KPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDM 553
           KPER      G D  KL+PFG+GRR+CPG G+AQR+V L L SL+QCFEWERV    +DM
Sbjct: 421 KPERFEKK--GEDK-KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDM 480

Query: 554 TEGE-GLTMPKAQPLVAKCKPRPILKAIL 578
            E E G TM KA  L A CK RPI+  +L
Sbjct: 481 RESEKGTTMRKATSLQAMCKARPIVHKVL 504

BLAST of Moc02g12450 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 520.8 bits (1340), Expect = 1.4e-147
Identity = 268/498 (53.82%), Postives = 350/498 (70.28%), Query Frame = 0

Query: 83  ILLLSISLSLFSLFIFFHFLLRPYRR-NLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHN 142
           IL+LS  LSLF+L IF   L R  R+ NLPPSP   LP+IGHLHL+K P+HRT   LS +
Sbjct: 6   ILILSF-LSLFTLAIF--LLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKS 65

Query: 143 YGH--VFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPY 202
            G+  VF LR G+RLV V+SS S  +ECFTKND+VLANRP     K L YN T L  + Y
Sbjct: 66  IGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASY 125

Query: 203 GEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHT 262
           G+HWRNLRRI+ +EIFST RLN FL IR+DE++ L+  L  DSL  F  VE+ ++L +  
Sbjct: 126 GDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLA 185

Query: 263 FNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEK 322
            N  +RM+AGK+Y+GED  D   +K  + +V E + + G  N  D++ +  W+  + YEK
Sbjct: 186 SNTTIRMLAGKRYFGEDNDD---AKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEK 245

Query: 323 RIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLA 382
           RI  +G R D  LQ+LVDE R +K+ G TMIDHLL+LQ+ QP+Y++D IIKG+   L++A
Sbjct: 246 RIKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIA 305

Query: 383 GIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLR 442
           G DT+++TLEWA+S LLN+PE+LKKAR EID  +G DRLV E D+ +LSYLQ ++ ETLR
Sbjct: 306 GTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLR 365

Query: 443 LNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG 502
           + PA PLL+PH +SEDCK+ GYDIP  T++L NAWA+HRD  +WED   FKPER      
Sbjct: 366 MYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKE-- 425

Query: 503 GVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPK 562
             ++ KL+ FG+GRRACPG G+A R++   L SL+QCFEWERV    VDMTE +G T+PK
Sbjct: 426 -GEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPK 485

Query: 563 AQPLVAKCKPRPILKAIL 578
           A PL A CK R I+  ++
Sbjct: 486 AIPLRAMCKARSIVDKLI 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149460.10.0e+00100.00isoflavone 2'-hydroxylase-like [Momordica charantia][more]
XP_022149455.11.3e-22778.27cytochrome P450 81E8-like [Momordica charantia][more]
XP_022947035.17.3e-21873.95cytochrome P450 81F3 [Cucurbita moschata][more]
XP_023007415.12.8e-21773.55cytochrome P450 81F3-like [Cucurbita maxima][more]
XP_023532689.12.8e-21773.75cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.... [more]
Match NameE-valueIdentityDescription
W8JMU72.8e-16457.34Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q9FG656.1e-15155.29Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
P931471.0e-15053.60Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q6WNQ87.5e-14954.02Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
Q9LHA16.3e-14852.46Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D5S60.0e+00100.00isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... [more]
A0A6J1D5S16.4e-22878.27cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV... [more]
A0A6J1G5P73.5e-21873.95cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1[more]
A0A6J1L2W81.3e-21773.55cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1[more]
A0A6J1D7975.1e-21773.29isoflavone 3'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017936 PE... [more]
Match NameE-valueIdentityDescription
AT5G36220.14.3e-15255.29cytochrome p450 81d1 [more]
AT4G37370.12.2e-15154.49cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37320.14.8e-15153.48cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
AT3G28740.14.5e-14952.46Cytochrome P450 superfamily protein [more]
AT4G37330.11.4e-14753.82cytochrome P450, family 81, subfamily D, polypeptide 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 139..158
score: 41.14
coord: 506..516
score: 56.69
coord: 163..184
score: 21.47
coord: 258..276
score: 21.16
coord: 368..385
score: 26.93
coord: 472..496
score: 29.07
coord: 388..414
score: 40.74
coord: 516..539
score: 34.06
coord: 431..449
score: 37.82
IPR001128Cytochrome P450PRINTSPR00385P450coord: 507..516
score: 60.23
coord: 432..443
score: 42.74
coord: 516..527
score: 30.1
coord: 379..396
score: 39.39
IPR001128Cytochrome P450PFAMPF00067p450coord: 112..560
e-value: 1.5E-93
score: 314.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 103..570
e-value: 9.2E-121
score: 405.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 111..570
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 85..544
e-value: 6.2E-20
score: 69.4
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 88..575
NoneNo IPR availablePANTHERPTHR24298:SF836CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATEDcoord: 88..575
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 509..518

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g12450.1Moc02g12450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen