Moc02g12400 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g12400
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	cytochrome P450 81D1-like
Location	chr2: 9017648 .. 9018160 (+)
RNA-Seq Expression	Moc02g12400
Synteny	Moc02g12400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGATCATAGAAACCAAACCATGGATGATATTTTTCTTTACCTTGGTCTCTTTCTGATCTCTCTCCTGGCTGCTGTCAAATTTTTGCTGCAGCCAGGTCAAAACCAAAACCTCCCGCCGAGCCCACTTTTTTGTCTTCCCTTAATAGGCCACCTCCATCTCATCAAACCTCCACTCCACCGGAAACTTCACGAACTCTCGGAGAAGTATGGCCCCATCTTCTCTCTCCGGTTCGGCTCCCGTCTTGTTGTAGTTGTATCATCTCCTTCCACTGTACGTGAATGCTTCACGAAGAATGATATCGTTCTTGCCAATCGGCCGTTCTTGAACACTGGAAAGCACTTGGCCTATAACTTTACAATACTCGCTGTCGCCCCTTACGGCGAGCTTTGGCGCAACCTCCGCCGCATCAGCACCCACGAGATTTTCTCGGCGACACGCCTTAATCTGTTCTCGGGAATCAGACAAGACGAAGTCCAGCGGTTGCTGCGTGCGTTAAGCGGAAATTAG

mRNA sequence

ATGTCTGATCATAGAAACCAAACCATGGATGATATTTTTCTTTACCTTGGTCTCTTTCTGATCTCTCTCCTGGCTGCTGTCAAATTTTTGCTGCAGCCAGGTCAAAACCAAAACCTCCCGCCGAGCCCACTTTTTTGTCTTCCCTTAATAGGCCACCTCCATCTCATCAAACCTCCACTCCACCGGAAACTTCACGAACTCTCGGAGAAGTATGGCCCCATCTTCTCTCTCCGGTTCGGCTCCCGTCTTGTTGTAGTTGTATCATCTCCTTCCACTGTACGTGAATGCTTCACGAAGAATGATATCGTTCTTGCCAATCGGCCGTTCTTGAACACTGGAAAGCACTTGGCCTATAACTTTACAATACTCGCTGTCGCCCCTTACGGCGAGCTTTGGCGCAACCTCCGCCGCATCAGCACCCACGAGATTTTCTCGGCGACACGCCTTAATCTGTTCTCGGGAATCAGACAAGACGAAGTCCAGCGGTTGCTGCGTGCGTTAAGCGGAAATTAG

Coding sequence (CDS)

ATGTCTGATCATAGAAACCAAACCATGGATGATATTTTTCTTTACCTTGGTCTCTTTCTGATCTCTCTCCTGGCTGCTGTCAAATTTTTGCTGCAGCCAGGTCAAAACCAAAACCTCCCGCCGAGCCCACTTTTTTGTCTTCCCTTAATAGGCCACCTCCATCTCATCAAACCTCCACTCCACCGGAAACTTCACGAACTCTCGGAGAAGTATGGCCCCATCTTCTCTCTCCGGTTCGGCTCCCGTCTTGTTGTAGTTGTATCATCTCCTTCCACTGTACGTGAATGCTTCACGAAGAATGATATCGTTCTTGCCAATCGGCCGTTCTTGAACACTGGAAAGCACTTGGCCTATAACTTTACAATACTCGCTGTCGCCCCTTACGGCGAGCTTTGGCGCAACCTCCGCCGCATCAGCACCCACGAGATTTTCTCGGCGACACGCCTTAATCTGTTCTCGGGAATCAGACAAGACGAAGTCCAGCGGTTGCTGCGTGCGTTAAGCGGAAATTAG

Protein sequence

MSDHRNQTMDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELSEKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPYGELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALSGN

Homology

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BLAST of Moc02g12400 vs. NCBI nr
Match: XP_038901746.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 243.8 bits (621), Expect = 1.1e-60
Identity = 126/163 (77.30%), Postives = 140/163 (85.89%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELS 68
           MD+I +Y+ LF+ISLL A+K LL  G  +NLPPSPLF LPLIGHLHL+K P+H+ LH  S
Sbjct: 1   MDNILVYIFLFVISLLLALKLLLPHG--KNLPPSPLFSLPLIGHLHLLKHPIHQNLHNHS 60

Query: 69  EKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPY 128
            KYG IFSL FGSRLVVVVSSPS VRECFTKNDIVLANRPFLNTGKHL YNFT+LAVAPY
Sbjct: 61  NKYGRIFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLAVAPY 120

Query: 129 GELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLL-RALSGN 171
           GELWR+LRRIST EIFS +RLNLFSGIRQDEV+RLL +  SGN
Sbjct: 121 GELWRSLRRISTCEIFSTSRLNLFSGIRQDEVKRLLCKLCSGN 161

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BLAST of Moc02g12400 vs. NCBI nr
Match: XP_011650030.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN63296.1 hypothetical protein Csa_022604 [Cucumis sativus])

HSP 1 Score: 232.6 bits (592), Expect = 2.4e-57
Identity = 115/158 (72.78%), Postives = 134/158 (84.81%), Query Frame = 0


Query: 10  DDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELSE 69
           ++I +Y+  FLISLL  +K LL  G  +NLPP+PLF LPLIGHLHL+K P+H+ LH LS+
Sbjct: 3   NNILVYIFFFLISLLLGLKLLLHHG--KNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 62

Query: 70  KYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPYG 129
           KYG +FSLRFGS LVVV+SSPS VRECFTKNDI+LANRPFLNTGKHL+YNFT+LAVAPYG
Sbjct: 63  KYGHVFSLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 122

Query: 130 ELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRAL 168
           ELWR LRRIST EIFS ++LN FS IRQDEV+RLL  L
Sbjct: 123 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKL 158

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BLAST of Moc02g12400 vs. NCBI nr
Match: XP_023512110.1 (cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 232.6 bits (592), Expect = 2.4e-57
Identity = 116/156 (74.36%), Postives = 131/156 (83.97%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELS 68
           M  I LY+ LF I+LL A+KF   P   +NLPP+PLF LP+IGHLHL+K P+HR LH+LS
Sbjct: 1   MAHILLYIFLFPITLLLALKFF--PRHAKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLS 60

Query: 69  EKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPY 128
            K+G +FSLRFGSRLVVV+SSPS VRECFT NDIVLANRPFLNTGKHL YNFT+LAVAPY
Sbjct: 61  NKHGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPY 120

Query: 129 GELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLL 165
           GELWR LRRIST EIFS  RL+L SGIRQDEV+RLL
Sbjct: 121 GELWRTLRRISTREIFSTCRLDLSSGIRQDEVKRLL 154

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BLAST of Moc02g12400 vs. NCBI nr
Match: XP_022943756.1 (cytochrome P450 81D1-like [Cucurbita moschata])

HSP 1 Score: 232.3 bits (591), Expect = 3.2e-57
Identity = 116/156 (74.36%), Postives = 130/156 (83.33%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELS 68
           M  I LY+ LF I+ L A+KF   P   +NLPP+PLF LP+IGHLHL+K P+HR LH LS
Sbjct: 1   MAHILLYIFLFPITFLLALKFF--PRHVKNLPPTPLFSLPIIGHLHLLKHPIHRTLHHLS 60

Query: 69  EKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPY 128
            K+G +FSLRFGSRLVVV+SSPS VRECFT NDIVLANRPFLNTGKHL YNFT+LAVAPY
Sbjct: 61  NKHGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPY 120

Query: 129 GELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLL 165
           GELWR LRRIST EIFS TRL+L SGIRQDEV+RLL
Sbjct: 121 GELWRTLRRISTREIFSTTRLDLSSGIRQDEVKRLL 154

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BLAST of Moc02g12400 vs. NCBI nr
Match: XP_022985606.1 (cytochrome P450 81D1-like [Cucurbita maxima])

HSP 1 Score: 231.1 bits (588), Expect = 7.1e-57
Identity = 115/156 (73.72%), Postives = 130/156 (83.33%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELS 68
           M  I LY+ LF I+LL A+KF   P   +NLPP+PLF LP+IGHLHL+K P+HR LH+LS
Sbjct: 1   MAHILLYIFLFPITLLLALKFF--PRHGKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLS 60

Query: 69  EKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPY 128
            K+G +FSLRFGSRLVVVVSSPS V ECFT NDIVLANRPFLNTGKHL YNFT+LAVAPY
Sbjct: 61  NKHGHVFSLRFGSRLVVVVSSPSAVHECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPY 120

Query: 129 GELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLL 165
           GELWR LRRIST EIFS  RL+LF GIRQ+EV+RLL
Sbjct: 121 GELWRTLRRISTREIFSTCRLDLFLGIRQEEVKRLL 154

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BLAST of Moc02g12400 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 6.7e-42
Identity = 85/154 (55.19%), Postives = 111/154 (72.08%), Query Frame = 0


Query: 14  LYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELSEKYGP 73
           LY  L ++ L  ++K      + +NLPPSP   LP+IGHLHLI   LHR L++LS+KYG 
Sbjct: 7   LYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGS 66

Query: 74  IFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPYGELWR 133
           +FSL+ G+RLV+VVSSP+   ECFTKNDIV ANRP    GK++ YN+T +  +PYGE WR
Sbjct: 67  VFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWR 126

Query: 134 NLRRISTHEIFSATRLNLFSGIRQDEVQRLLRAL 168
           NLRR++  EIFSA  LN F  IR+DEV++LL +L
Sbjct: 127 NLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSL 160

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BLAST of Moc02g12400 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 9.7e-41
Identity = 90/165 (54.55%), Postives = 109/165 (66.06%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQ-NLPPSPLFCLPLIGHLHLIKPPLHRKLHEL 68
           M+ I+L L LF   +  ++K L  P   + NLPPSP    P+IGHLHL+K PLHR+   L
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 69  SEKY--GPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAV 128
           SE      IFSL  GSRLV VVSS +   ECFTKND+VLANRP    GKH+ YN T +  
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 129 APYGELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALSGN 171
           A YG+ WRNLRRI T EIFS+ RLN F  IRQDE++RL+  L+ N
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKN 172

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BLAST of Moc02g12400 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 2.2e-40
Identity = 88/161 (54.66%), Postives = 112/161 (69.57%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQN-QNLPPSPLFCLPLIGHLHLIKPPLHRKLHEL 68
           M   +L L + L  L+  +K      +  +NLPP P  CLP+IG+LH +K PLH   H L
Sbjct: 1   MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTL 60

Query: 69  SEKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAP 128
           S+KYG IFSL FGSRLVVVVSS +  +ECFTKNDIVLANRP   TGK++ YN T +A +P
Sbjct: 61  SQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSP 120

Query: 129 YGELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALS 169
           YG+ WRNLRRI + EI S+ RLN F  IR+DE+ RL++ L+
Sbjct: 121 YGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLA 160

[Close]

BLAST of Moc02g12400 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.8e-39
Identity = 85/155 (54.84%), Postives = 108/155 (69.68%), Query Frame = 0


Query: 15  YLGLFLISLLAAVKFLLQ--PGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELSEKYG 74
           Y  L  +S +  +K LL+    + +NLPP P   +P+IG+LH +K PLHR    LS+ YG
Sbjct: 4   YYSLLSLSFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYG 63

Query: 75  PIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPYGELW 134
            IFSL FGSRLVVVVSSPS   ECFTKNDI+LANRP   TGK++ YN+T L  A YG+ W
Sbjct: 64  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 123

Query: 135 RNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRAL 168
           RNLRRI+T ++ S  RLN F G+R+DE  RL++ L
Sbjct: 124 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKL 157

[Close]

BLAST of Moc02g12400 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.2e-38
Identity = 84/158 (53.16%), Postives = 109/158 (68.99%), Query Frame = 0


Query: 12  IFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELSEKY 71
           I L L LF   LLA   F     +  NLPPSP + LP++GH +L+KPP+HR  H LS+ +
Sbjct: 6   IVLPLALF---LLAYKLFFTSKTKRFNLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTH 65

Query: 72  GPIFSLRFGSRLVVVVSSPSTVRECFT-KNDIVLANRPFLNTGKHLAYNFTILAVAPYGE 131
           GPIFSL+FGSR  VV+SS S   +CFT +NDI+L+NRP   T K++AYN+T +  APYG+
Sbjct: 66  GPIFSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGD 125

Query: 132 LWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALS 169
            WRNLRRI + EI S+ RL  F  IR+DE+ R+L  LS
Sbjct: 126 HWRNLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLS 160

[Close]

BLAST of Moc02g12400 vs. ExPASy TrEMBL
Match: A0A0A0LTD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.2e-57
Identity = 115/158 (72.78%), Postives = 134/158 (84.81%), Query Frame = 0


Query: 10  DDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELSE 69
           ++I +Y+  FLISLL  +K LL  G  +NLPP+PLF LPLIGHLHL+K P+H+ LH LS+
Sbjct: 3   NNILVYIFFFLISLLLGLKLLLHHG--KNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 62

Query: 70  KYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPYG 129
           KYG +FSLRFGS LVVV+SSPS VRECFTKNDI+LANRPFLNTGKHL+YNFT+LAVAPYG
Sbjct: 63  KYGHVFSLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 122

Query: 130 ELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRAL 168
           ELWR LRRIST EIFS ++LN FS IRQDEV+RLL  L
Sbjct: 123 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKL 158

[Close]

BLAST of Moc02g12400 vs. ExPASy TrEMBL
Match: A0A6J1FSK8 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448412 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.5e-57
Identity = 116/156 (74.36%), Postives = 130/156 (83.33%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELS 68
           M  I LY+ LF I+ L A+KF   P   +NLPP+PLF LP+IGHLHL+K P+HR LH LS
Sbjct: 1   MAHILLYIFLFPITFLLALKFF--PRHVKNLPPTPLFSLPIIGHLHLLKHPIHRTLHHLS 60

Query: 69  EKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPY 128
            K+G +FSLRFGSRLVVV+SSPS VRECFT NDIVLANRPFLNTGKHL YNFT+LAVAPY
Sbjct: 61  NKHGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPY 120

Query: 129 GELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLL 165
           GELWR LRRIST EIFS TRL+L SGIRQDEV+RLL
Sbjct: 121 GELWRTLRRISTREIFSTTRLDLSSGIRQDEVKRLL 154

[Close]

BLAST of Moc02g12400 vs. ExPASy TrEMBL
Match: A0A6J1J8Q1 (cytochrome P450 81D1-like OS=Cucurbita maxima OX=3661 GN=LOC111483619 PE=3 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.4e-57
Identity = 115/156 (73.72%), Postives = 130/156 (83.33%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELS 68
           M  I LY+ LF I+LL A+KF   P   +NLPP+PLF LP+IGHLHL+K P+HR LH+LS
Sbjct: 1   MAHILLYIFLFPITLLLALKFF--PRHGKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLS 60

Query: 69  EKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPY 128
            K+G +FSLRFGSRLVVVVSSPS V ECFT NDIVLANRPFLNTGKHL YNFT+LAVAPY
Sbjct: 61  NKHGHVFSLRFGSRLVVVVSSPSAVHECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPY 120

Query: 129 GELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLL 165
           GELWR LRRIST EIFS  RL+LF GIRQ+EV+RLL
Sbjct: 121 GELWRTLRRISTREIFSTCRLDLFLGIRQEEVKRLL 154

[Close]

BLAST of Moc02g12400 vs. ExPASy TrEMBL
Match: A0A6J1JBS5 (cytochrome P450 81D11-like OS=Cucurbita maxima OX=3661 GN=LOC111483618 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 2.1e-54
Identity = 111/162 (68.52%), Postives = 130/162 (80.25%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELS 68
           MD IFL + L ++S+  A  FLL+P   QNLPP+P F LP+IGHLHLI+ PLHR LH LS
Sbjct: 1   MDIIFLSISLSMLSIFLAFIFLLRP-PRQNLPPTPFFSLPIIGHLHLIRHPLHRILHNLS 60

Query: 69  EKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPY 128
           + YG +FSLRFGSR+VVVVSSPS V+ECFTKNDIVLANRP L TGKHLAYN T +AVAPY
Sbjct: 61  KTYGHVFSLRFGSRIVVVVSSPSVVQECFTKNDIVLANRPLLGTGKHLAYNHTTMAVAPY 120

Query: 129 GELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALSGN 171
           G+ WRNLRRI   EIFS +RLNLF GIR+DEV+RL+  L G+
Sbjct: 121 GDHWRNLRRIGALEIFSTSRLNLFLGIREDEVKRLMLRLRGS 161

[Close]

BLAST of Moc02g12400 vs. ExPASy TrEMBL
Match: A0A0A0LNA4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 3.6e-54
Identity = 108/162 (66.67%), Postives = 130/162 (80.25%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELS 68
           MD + L + L L SLL A  FLL+P   QNLPP+P FCLP+IGHLHLIK PLHR LH LS
Sbjct: 1   MDVLILSISLSLFSLLLAFNFLLRP-HRQNLPPTPFFCLPVIGHLHLIKHPLHRILHNLS 60

Query: 69  EKYGPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAPY 128
           + YG +FSLRFGSRLVV++SSPS V+ECFTKNDI+LANRP L+TGKHLAYN T +AVAPY
Sbjct: 61  KSYGHVFSLRFGSRLVVIISSPSVVQECFTKNDIILANRPLLDTGKHLAYNHTTMAVAPY 120

Query: 129 GELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALSGN 171
           GE WRNLRRI   E+FS +R+NLFS +R++EV+ L+  L G+
Sbjct: 121 GEHWRNLRRIGALELFSTSRINLFSRMREEEVKALMVRLCGS 161

[Close]

BLAST of Moc02g12400 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 6.9e-42
Identity = 90/165 (54.55%), Postives = 109/165 (66.06%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQ-NLPPSPLFCLPLIGHLHLIKPPLHRKLHEL 68
           M+ I+L L LF   +  ++K L  P   + NLPPSP    P+IGHLHL+K PLHR+   L
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 69  SEKY--GPIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAV 128
           SE      IFSL  GSRLV VVSS +   ECFTKND+VLANRP    GKH+ YN T +  
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 129 APYGELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALSGN 171
           A YG+ WRNLRRI T EIFS+ RLN F  IRQDE++RL+  L+ N
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKN 172

[Close]

BLAST of Moc02g12400 vs. TAIR 10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )

HSP 1 Score: 167.5 bits (423), Expect = 9.0e-42
Identity = 89/161 (55.28%), Postives = 110/161 (68.32%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPP-LHRKLHEL 68
           MD I L L   L+++      LL    N+NLPPSP  C P+IGHLHL+K P LHR L  L
Sbjct: 1   MDCILLIL-TTLVAIFIVKIVLLVTKPNKNLPPSPNICFPIIGHLHLLKKPLLHRTLSHL 60

Query: 69  SEKYGPIFSLRFGSRLVVVVSSPSTVRECF-TKNDIVLANRPFLNTGKHLAYNFTILAVA 128
           S   GP+FSLR GSRL V++SSP+   ECF TKNDIVLANRP    GK++AY++T +  A
Sbjct: 61  SHSLGPVFSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTA 120

Query: 129 PYGELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRAL 168
           PYG+ WRNLRRI+  E+FS  RLN  + IR DEV+ LL+ L
Sbjct: 121 PYGDHWRNLRRITALEVFSTNRLNASAEIRHDEVKMLLQKL 160

[Close]

BLAST of Moc02g12400 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 166.4 bits (420), Expect = 2.0e-41
Identity = 85/161 (52.80%), Postives = 112/161 (69.57%), Query Frame = 0


Query: 11  DIFLYLGLFLISLLAAVKFLL-QPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELSE 70
           D+   L L  + L  ++K LL +  +  NLPPSP + LP+IGHLHL+K P+HR  H +S+
Sbjct: 2   DLTQILLLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISK 61

Query: 71  KYG--PIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAP 130
             G  PIF LR G+RLV V+SS S   ECFTKND+VLANRP +   KH+ YNFT +  A 
Sbjct: 62  SLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAAS 121

Query: 131 YGELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALS 169
           YG+ WRNLRRI+  EIFS+ R++ FS IR+DE++RL+  LS
Sbjct: 122 YGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLS 162

[Close]

BLAST of Moc02g12400 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 163.7 bits (413), Expect = 1.3e-40
Identity = 89/161 (55.28%), Postives = 106/161 (65.84%), Query Frame = 0


Query: 11  DIFLYLGLFLISLLAAVKFLLQPGQNQ-NLPPSPLFCLPLIGHLHLIKPPLHRKLHELSE 70
           D+   L L  +SL     FLL   + + NLPPSP   LP+IGHLHL+KPPLHR    LS+
Sbjct: 2   DLNQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSK 61

Query: 71  KYG--PIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVAP 130
             G  P+F LR G+RLV V+SS S   ECFTKND+VLANRP     KHL YN T L  A 
Sbjct: 62  SIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSAS 121

Query: 131 YGELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALS 169
           YG+ WRNLRRI+  EIFS  RLN F  IR+DE++RL+  LS
Sbjct: 122 YGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLS 162

[Close]

BLAST of Moc02g12400 vs. TAIR 10
Match: AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7 )

HSP 1 Score: 163.3 bits (412), Expect = 1.7e-40
Identity = 88/164 (53.66%), Postives = 105/164 (64.02%), Query Frame = 0


Query: 9   MDDIFLYLGLFLISLLAAVKFLLQPGQNQNLPPSPLFCLPLIGHLHLIKPPLHRKLHELS 68
           M+  FL L L  +  ++      +     NLPPSP   LP IGHLHL+K PLHR     S
Sbjct: 45  METHFLILSLAFLFFISLKLLFGKRHSKFNLPPSPARPLPFIGHLHLLKQPLHRTFLSFS 104

Query: 69  EKYG--PIFSLRFGSRLVVVVSSPSTVRECFTKNDIVLANRPFLNTGKHLAYNFTILAVA 128
           +  G  PIFSLR G+ L VVVSS S   ECFTKNDIVLANRP    GKH+ YNFT +  A
Sbjct: 105 QSLGDAPIFSLRLGNHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTSA 164

Query: 129 PYGELWRNLRRISTHEIFSATRLNLFSGIRQDEVQRLLRALSGN 171
           PYG+ WRNLRRI T EIFS+ +LN F  +R+DE++ LL  LS N
Sbjct: 165 PYGDHWRNLRRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRLSKN 208

The following BLAST results are available for this feature:

BLAST of Moc02g12400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901746.1	1.1e-60	77.30	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
XP_011650030.1	2.4e-57	72.78	cytochrome P450 81Q32 [Cucumis sativus] >KGN63296.1 hypothetical protein Csa_022...	[more]
XP_023512110.1	2.4e-57	74.36	cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022943756.1	3.2e-57	74.36	cytochrome P450 81D1-like [Cucurbita moschata]	[more]
XP_022985606.1	7.1e-57	73.72	cytochrome P450 81D1-like [Cucurbita maxima]	[more]

BLAST of Moc02g12400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	6.7e-42	55.19	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q9LHA1	9.7e-41	54.55	Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1	[more]
Q6WNQ8	2.2e-40	54.66	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNQ9	1.8e-39	54.84	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q0WTF4	1.2e-38	53.16	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]

BLAST of Moc02g12400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTD0	1.2e-57	72.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1	[more]
A0A6J1FSK8	1.5e-57	74.36	cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448412 PE=3 SV=...	[more]
A0A6J1J8Q1	3.4e-57	73.72	cytochrome P450 81D1-like OS=Cucurbita maxima OX=3661 GN=LOC111483619 PE=3 SV=1	[more]
A0A6J1JBS5	2.1e-54	68.52	cytochrome P450 81D11-like OS=Cucurbita maxima OX=3661 GN=LOC111483618 PE=3 SV=1	[more]
A0A0A0LNA4	3.6e-54	66.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1	[more]

BLAST of Moc02g12400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28740.1	6.9e-42	54.55	Cytochrome P450 superfamily protein	[more]
AT4G37310.1	9.0e-42	55.28	cytochrome P450, family 81, subfamily H, polypeptide 1	[more]
AT4G37320.1	2.0e-41	52.80	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT4G37330.1	1.3e-40	55.28	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT2G23190.1	1.7e-40	53.66	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 92..113 score: 22.15 coord: 68..87 score: 45.1
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 40..167 e-value: 1.1E-15 score: 57.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 33..170 e-value: 2.6E-28 score: 100.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 37..168
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 12..168
None	No IPR available	PANTHER	PTHR24298:SF840	P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 8, PUTATIVE-RELATED	coord: 12..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g12400.1	Moc02g12400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen