Moc02g12370 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc02g12370 vs. NCBI nr
Match: XP_023513339.1 (cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570.9 bits (1470), Expect = 8.9e-159
Identity = 307/497 (61.77%), Postives = 352/497 (70.82%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSEK 62
           MENL+L LC+ L  FI ALIFLLR    LPPSPPFSLP+IGHLYL+KRPVH+TLQ LS K
Sbjct: 1   MENLMLCLCLPLMAFISALIFLLRPSTKLPPSPPFSLPIIGHLYLLKRPVHQTLQKLSNK 60

Query: 63  YGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYGE 122
           YG VF+LRLGS LVVV+SSPS+VKECFT+NDI+LANRP SNI KHLTYNNTIFSASPYG+
Sbjct: 61  YGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASPYGD 120

Query: 123 HWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTSN 182
           HWRELRRIS +EIFSATRLN+FLETRKDEIK LL KL   S+  FVRVEM SM  EL+SN
Sbjct: 121 HWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLCCISSDSFVRVEMSSMISELSSN 180

Query: 183 IVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKRT 242
           IVMRMV GKRFYGE VSD EQAR+FR IVKQVM  CG+++SR+F+PLLNW DITHYEKR 
Sbjct: 181 IVMRMVMGKRFYGEGVSDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDITHYEKRI 240

Query: 243 VRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLAG 302
           ++LGQ+ DEFLQGLIDE RNQKEEG N MIDHLLSLQ+SQL+++SDQVIKGLI VILLAG
Sbjct: 241 IKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQLDFMSDQVIKGLITVILLAG 300

Query: 303 TDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-------- 362
           TDTIAVTIEWALSHLLNNP+VLKKAR ELDS++G+ERLV+ESD+SKL YLQG        
Sbjct: 301 TDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVEESDMSKLSYLQGVISETLRL 360

Query: 363 ------------------------------------------------------------ 398
                                                                       
Sbjct: 361 NPAAPLLVPHYASENCSIAGYEIPRDTIILINAWAIHRDPNIWEDANTFNPERYKITMVD 420

BLAST of Moc02g12370 vs. NCBI nr
Match: XP_022944471.1 (cytochrome P450 81D1-like [Cucurbita moschata])

HSP 1 Score: 565.1 bits (1455), Expect = 4.9e-157
Identity = 303/497 (60.97%), Postives = 351/497 (70.62%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSEK 62
           MENL+LYLC+ L  FI +LIFLLR    LPPSPPFSLP+IGHLYL+KRPVH+TLQ LS K
Sbjct: 1   MENLMLYLCLPLMAFISSLIFLLRPSTKLPPSPPFSLPIIGHLYLLKRPVHQTLQKLSNK 60

Query: 63  YGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYGE 122
           YG VFSLRLGS LVVV+SSPS VKECFT+NDI+LANRP SNI KHLTYNNTIFSAS YG+
Sbjct: 61  YGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASSYGD 120

Query: 123 HWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTSN 182
           HWRELRRIS +EIFSA+RLN+FLETRKDEIKR+L KL   S+  F RVEM SM  EL+SN
Sbjct: 121 HWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRISSDSFARVEMSSMISELSSN 180

Query: 183 IVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKRT 242
           IVMRMV GKRFYGE V D EQAR+FR IVKQVM  CG+++SR+F+PLLNW DITHYEK+ 
Sbjct: 181 IVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDITHYEKKI 240

Query: 243 VRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLAG 302
           ++LGQ+ DEFLQGLIDE RNQKEEG N MIDHLLSLQ+SQL+++SDQVIKGLIMVILLAG
Sbjct: 241 IKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQLDFMSDQVIKGLIMVILLAG 300

Query: 303 TDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-------- 362
           TDTIAVTIEWALSHLLNNP+VLKKAR ELDS++G+ERLV+ESD+SKL YLQG        
Sbjct: 301 TDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVEESDMSKLSYLQGVISETLRL 360

Query: 363 ------------------------------------------------------------ 398
                                                                       
Sbjct: 361 NPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDPNIWEDANTFNPERYKITMVD 420

BLAST of Moc02g12370 vs. NCBI nr
Match: XP_038902550.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 538.9 bits (1387), Expect = 3.7e-149
Identity = 298/498 (59.84%), Postives = 340/498 (68.27%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSEK 62
           MENL+LYL +SL  FI AL FL++   NLPPSPPFSLP+IGHLYL+KRP+H+TLQ LS+K
Sbjct: 1   MENLMLYLFLSLIIFISALKFLVKPSINLPPSPPFSLPIIGHLYLLKRPIHQTLQKLSKK 60

Query: 63  YGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYGE 122
           YG VFSLRLGS LVVV+SSPS VKECFTVNDI LANRP +NITKHLTYNNTIFSAS YG+
Sbjct: 61  YGHVFSLRLGSQLVVVVSSPSAVKECFTVNDITLANRPQTNITKHLTYNNTIFSASSYGD 120

Query: 123 HWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTSN 182
           HWR+LRRIS +EIFS TRLN+FLE RKDEIKRLL KL   S+  F  VEM SMF +L+SN
Sbjct: 121 HWRDLRRISTIEIFSTTRLNMFLEIRKDEIKRLLGKLCGISSDDFASVEMGSMFSKLSSN 180

Query: 183 IVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKRT 242
           IVMRMV GKRFYGEDV DAEQA+EFR IVKQVM NCG+Y+S + + LLNW DIT YEKR 
Sbjct: 181 IVMRMVTGKRFYGEDVLDAEQAKEFRDIVKQVMENCGSYDSSSIL-LLNWIDITGYEKRI 240

Query: 243 VRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLAG 302
           + LGQ+ D+FLQGLIDE RNQKEEG N MIDHLLSLQESQL+ +SDQVIKGLIMVILLAG
Sbjct: 241 IELGQRNDQFLQGLIDEHRNQKEEGRNTMIDHLLSLQESQLDCMSDQVIKGLIMVILLAG 300

Query: 303 TDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-------- 362
           TDT+AVT+EWALSHLLNNPDVL  AR ELDS+VG+ +LVDESDLSKL YLQG        
Sbjct: 301 TDTVAVTMEWALSHLLNNPDVLNNARIELDSIVGQGQLVDESDLSKLSYLQGIIYETLRL 360

Query: 363 ------------------------------------------------------------ 398
                                                                       
Sbjct: 361 NPAAPLLVPHYASKNCSIAGYEIPRDTMILINAWAIHRDPNIWEDATTFEPKRYKNRNVD 420

BLAST of Moc02g12370 vs. NCBI nr
Match: XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 436.4 bits (1121), Expect = 2.6e-118
Identity = 247/498 (49.60%), Postives = 305/498 (61.24%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILALIFLLRS-PKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSE 62
           M+ LLL + +SL +  +   FLLR   +NLPPSP F LPLIGHL+L+K PVHRTL  LS 
Sbjct: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140

Query: 63  KYGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYG 122
            YG VFSLR GS LVVV+SSPS V+ECFT NDIVLANRPL    K L YN+T  + SPYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200

Query: 123 EHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTS 182
           EHWR LRRIS LEIFS +RLN+FL  R+DE+K LL KLR  S  +F  VE++SM ++ T 
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260

Query: 183 NIVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKR 242
           NIV+RM+AGK++YGED+ D  Q+++FR +VK+++AN G  N  +FVPL NW D T YEKR
Sbjct: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320

Query: 243 TVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLA 302
            +++G+++DE LQ L+DE RNQK +G N MIDHLLSLQ +Q  Y SDQ+IKGL  ++LLA
Sbjct: 321 IMKIGERSDELLQELVDEIRNQK-DGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLA 380

Query: 303 GTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG------- 362
           G DT A+T+EWALS LLNNP+VLKKAR E+D  +G++RLV E DLS L YLQG       
Sbjct: 381 GIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLR 440

Query: 363 ------------------------------------------------------------ 397
                                                                       
Sbjct: 441 LNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG 500

BLAST of Moc02g12370 vs. NCBI nr
Match: XP_022985606.1 (cytochrome P450 81D1-like [Cucurbita maxima])

HSP 1 Score: 431.4 bits (1108), Expect = 8.4e-117
Identity = 243/497 (48.89%), Postives = 304/497 (61.17%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSEK 62
           M ++LLY+ +   T +LAL F  R  KNLPP+P FSLP+IGHL+L+K P+HRTL  LS K
Sbjct: 1   MAHILLYIFLFPITLLLALKFFPRHGKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLSNK 60

Query: 63  YGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYGE 122
           +G VFSLR GS LVVV+SSPS V ECFT NDIVLANRP  N  KHLTYN T+ + +PYGE
Sbjct: 61  HGHVFSLRFGSRLVVVVSSPSAVHECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 123 HWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTSN 182
            WR LRRIS  EIFS  RL++FL  R++E+KRLLCKL  G+    V VE++ M M+LTSN
Sbjct: 121 LWRTLRRISTREIFSTCRLDLFLGIRQEEVKRLLCKLCRGNT---VVVEVQPMIMDLTSN 180

Query: 183 IVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKRT 242
           IVMRMVAGKR++G+DVSD EQA  FR +VK+VM   G  N  +F+PL NW D + +EK+ 
Sbjct: 181 IVMRMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKI 240

Query: 243 VRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLAG 302
           +++G++AD   QGLIDE RNQK  G N MIDHLL LQ +Q  Y SDQ+IKGLI VILLAG
Sbjct: 241 MKVGEEADAIFQGLIDEIRNQKGSG-NTMIDHLLHLQTTQSEYFSDQIIKGLIHVILLAG 300

Query: 303 TDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-------- 362
            DT AVT+EWALSHLLNNP+V+KKAR E++   G++RLV+E DLS L Y+QG        
Sbjct: 301 IDTTAVTLEWALSHLLNNPEVIKKARFEIERCTGQDRLVNEEDLSSLSYVQGIILETLRL 360

Query: 363 ------------------------------------------------------------ 397
                                                                       
Sbjct: 361 TPAAPLLVPHCASTDCKIQGYDVPRDTIIFVNAWAIHRDPNHWEDVTSFRPERHENAIEL 420

BLAST of Moc02g12370 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.5e-100
Identity = 217/498 (43.57%), Postives = 286/498 (57.43%), Query Frame = 0

Query: 1   MEMENLL-LYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSL 60
           ME   LL  +L V L +  L L  + R  +NLPPSP  +LP+IGHL+LI + +HR+L  L
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60

Query: 61  SEKYGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASP 120
           S+KYG VFSL+LG+ LV+V+SSP+  +ECFT NDIV ANRPL  + K++ YN T    SP
Sbjct: 61  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120

Query: 121 YGEHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMEL 180
           YGEHWR LRR++ +EIFSA  LN FL  R+DE+K+LL  L   S   F +VEM+S   EL
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSEL 180

Query: 181 TSNIVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYE 240
           + N+ MRMVAGKR++G+DV D+++A+ FR ++ +V  + G  N  +FVP L W D  +YE
Sbjct: 181 SFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYE 240

Query: 241 KRTVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVIL 300
           K+  ++ Q+ D FLQ LI E R  K      MIDHLLSLQESQ  Y +DQ+IKG+IMV+L
Sbjct: 241 KKVSKISQEMDAFLQRLIHESRINK--NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLL 300

Query: 301 LAGTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYL------- 360
           LAGTDT AVT+EWA+S LLN+P+ L+KARTE+++ VG  RL++E DL KL YL       
Sbjct: 301 LAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISET 360

Query: 361 ---------------------QG------------------------------------- 397
                                QG                                     
Sbjct: 361 FRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGV 420

BLAST of Moc02g12370 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 5.0e-96
Identity = 212/504 (42.06%), Postives = 288/504 (57.14%), Query Frame = 0

Query: 1   MEMENL--LLYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQS 60
           ME  N+  +LY   SL   I++  FL    +NLPPSPP  LP+IGHL L+K P+HRTL+S
Sbjct: 1   MEETNIRVVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRS 60

Query: 61  LSEKY-----GPVFSLRLGSHLVVVISSPSI-VKECFTVNDIVLANRPLSNITKHLTYNN 120
            SE       G V SLRLGS LV V+SS  +  +ECF  ND+VLANRP   I KH+ YNN
Sbjct: 61  FSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNN 120

Query: 121 TIFSASPYGEHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEM 180
           T   A+PYG+HWR LRR+  +EIFS  RLN FL  R DE++RL+ +L   +  K   VE+
Sbjct: 121 TNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVEL 180

Query: 181 ESMFMELTSNIVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNW 240
           + M M+LT N +MRM+ GKR+YGE+ +D E+A+  R +V  V AN  + N+ ++VP+L  
Sbjct: 181 KPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRL 240

Query: 241 FDITHYEKRTVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIK 300
           F  + YE R  +LG++ D+FLQGLID++R Q+E G   MIDHLL LQ+S + Y +DQ+IK
Sbjct: 241 F--SSYENRVKKLGEETDKFLQGLIDDKRGQQETG-TTMIDHLLVLQKSDIEYYTDQIIK 300

Query: 301 GLIMVILLAGTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYL 360
           G+I+++++AGT+T AVT+EWALS+LLN+PDV+ KAR E+D+ VG +RL++E+DLS+L YL
Sbjct: 301 GIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYL 360

Query: 361 QG---------------------------------------------------------- 397
           +                                                           
Sbjct: 361 KNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFK 420

BLAST of Moc02g12370 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.5e-95
Identity = 196/367 (53.41%), Postives = 258/367 (70.30%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILAL-IFL--LRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSL 62
           M    L L +SL   I+ L +F    R  KNLPP P   LP+IG+L+ +K+P+H T  +L
Sbjct: 1   MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTL 60

Query: 63  SEKYGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASP 122
           S+KYG +FSL  GS LVVV+SS +I +ECFT NDIVLANRP     K++ YNNT  + SP
Sbjct: 61  SQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSP 120

Query: 123 YGEHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMEL 182
           YG+HWR LRRI  +EI S+ RLN FLE R+DEI RL+ KL   S + F  VE+  MF E+
Sbjct: 121 YGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEM 180

Query: 183 TSNIVMRMVAGKRFYGE--DVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITH 242
           T N +MRMV+GKR+YG   DVSD E+AR FRGI+K+V++  G  N  +F+  L WFD   
Sbjct: 181 TFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDG 240

Query: 243 YEKRTVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMV 302
            EKR  ++ ++ D FLQGLIDE R  K    N MIDHLL+ Q+SQ  Y +DQ+IKGL++V
Sbjct: 241 LEKRLKKISKRTDAFLQGLIDEHRFGK-RNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVV 300

Query: 303 ILLAGTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-CF 362
           +LLAGTDT +VTIEWA+S+LLN+P+++KKA+ ELD+ +G +R VDE D+SKL YLQ   +
Sbjct: 301 MLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVY 360

Query: 363 EWERLNS 364
           E  RL++
Sbjct: 361 ETLRLHA 365

BLAST of Moc02g12370 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.3e-91
Identity = 199/496 (40.12%), Postives = 282/496 (56.85%), Query Frame = 0

Query: 6   LLLYLCVSL----TTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSE 65
           +L YLC SL      FI+ L+F  R  KNLPP P  SLP+IG+L+ +KRP++RT ++L+E
Sbjct: 3   ILSYLCYSLFYLSIFFIIRLLFQSRKFKNLPPGPT-SLPIIGNLHHLKRPLNRTFKALTE 62

Query: 66  KYGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYG 125
           KYG V SL  GS LVVV+SS S  +ECFT ND+VLANRP     K++ YN T   ++ YG
Sbjct: 63  KYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 122

Query: 126 EHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTS 185
           EHWR LRRI+ L++ S  R+N F   R+DE +RL+ KL   S+ KF  VE+   F ++T 
Sbjct: 123 EHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTF 182

Query: 186 NIVMRMVAGKRFYGE--DVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYE 245
           N +MRM++GKR+YG+  D+S+ ++A +FR +V +++   G  N  +F+PLL + D  + E
Sbjct: 183 NNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLE 242

Query: 246 KRTVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVIL 305
           KR  R+G++ D FL GL+ E+R++KE   N MIDHLL++QESQ  Y +D +IKGL + +L
Sbjct: 243 KRVKRIGEKNDVFLSGLLQEQRSKKER-TNTMIDHLLNMQESQPEYYTDTIIKGLCLAML 302

Query: 306 LAGTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG----- 365
           LAGTD+ AVT+EW +S++LN P+VLKK R E+D+ VG++RLVDESDL KL YL+      
Sbjct: 303 LAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYET 362

Query: 366 ------------------------------------------------------------ 396
                                                                       
Sbjct: 363 LRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKK 422

BLAST of Moc02g12370 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 4.8e-91
Identity = 198/498 (39.76%), Postives = 278/498 (55.82%), Query Frame = 0

Query: 1   MEMENLLLYLCVSLTT-FILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSL 60
           ME+ +LL Y    L   FI  ++   R  KNLPP PP SLP+IG+L+ +KRP+HRT + L
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGL 60

Query: 61  SEKYGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASP 120
           SEKYG VFSL  GS LVVV+SS S  ++CFT ND+VLANRP     K++ YN T   ++ 
Sbjct: 61  SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120

Query: 121 YGEHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMEL 180
           YGEHWR LRRI+ L++ S  R+N F   R+DE +RL+ +L   S+  F  +E+ S   ++
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 180

Query: 181 TSNIVMRMVAGKRFYGE--DVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITH 240
           T N +MRM++GKR+YGE  D SD ++A +FR +V +++   G  N  +F+PLL + D  +
Sbjct: 181 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 240

Query: 241 YEKRTVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMV 300
            EKR   +  + D FL+GLI+E R +KE   N MIDHLL+LQ+SQ  Y +DQ+IKGL + 
Sbjct: 241 LEKRLKDISGKTDAFLRGLIEEHRTKKERA-NTMIDHLLNLQDSQPEYYTDQIIKGLALA 300

Query: 301 ILLAGTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG--- 360
           +LLAGTD+ AVT+EW++S+LLN+P+VLKK + ELD+ VG++RLVDESDL KL YL+    
Sbjct: 301 MLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVIN 360

Query: 361 ------------------------------------------------------------ 396
                                                                       
Sbjct: 361 ETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE 420

BLAST of Moc02g12370 vs. ExPASy TrEMBL
Match: A0A6J1FUI0 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448921 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.4e-157
Identity = 303/497 (60.97%), Postives = 351/497 (70.62%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSEK 62
           MENL+LYLC+ L  FI +LIFLLR    LPPSPPFSLP+IGHLYL+KRPVH+TLQ LS K
Sbjct: 1   MENLMLYLCLPLMAFISSLIFLLRPSTKLPPSPPFSLPIIGHLYLLKRPVHQTLQKLSNK 60

Query: 63  YGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYGE 122
           YG VFSLRLGS LVVV+SSPS VKECFT+NDI+LANRP SNI KHLTYNNTIFSAS YG+
Sbjct: 61  YGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASSYGD 120

Query: 123 HWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTSN 182
           HWRELRRIS +EIFSA+RLN+FLETRKDEIKR+L KL   S+  F RVEM SM  EL+SN
Sbjct: 121 HWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRISSDSFARVEMSSMISELSSN 180

Query: 183 IVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKRT 242
           IVMRMV GKRFYGE V D EQAR+FR IVKQVM  CG+++SR+F+PLLNW DITHYEK+ 
Sbjct: 181 IVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDITHYEKKI 240

Query: 243 VRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLAG 302
           ++LGQ+ DEFLQGLIDE RNQKEEG N MIDHLLSLQ+SQL+++SDQVIKGLIMVILLAG
Sbjct: 241 IKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQLDFMSDQVIKGLIMVILLAG 300

Query: 303 TDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-------- 362
           TDTIAVTIEWALSHLLNNP+VLKKAR ELDS++G+ERLV+ESD+SKL YLQG        
Sbjct: 301 TDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVEESDMSKLSYLQGVISETLRL 360

Query: 363 ------------------------------------------------------------ 398
                                                                       
Sbjct: 361 NPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDPNIWEDANTFNPERYKITMVD 420

BLAST of Moc02g12370 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 1.3e-118
Identity = 247/498 (49.60%), Postives = 305/498 (61.24%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILALIFLLRS-PKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSE 62
           M+ LLL + +SL +  +   FLLR   +NLPPSP F LPLIGHL+L+K PVHRTL  LS 
Sbjct: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140

Query: 63  KYGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYG 122
            YG VFSLR GS LVVV+SSPS V+ECFT NDIVLANRPL    K L YN+T  + SPYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200

Query: 123 EHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTS 182
           EHWR LRRIS LEIFS +RLN+FL  R+DE+K LL KLR  S  +F  VE++SM ++ T 
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260

Query: 183 NIVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKR 242
           NIV+RM+AGK++YGED+ D  Q+++FR +VK+++AN G  N  +FVPL NW D T YEKR
Sbjct: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320

Query: 243 TVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLA 302
            +++G+++DE LQ L+DE RNQK +G N MIDHLLSLQ +Q  Y SDQ+IKGL  ++LLA
Sbjct: 321 IMKIGERSDELLQELVDEIRNQK-DGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLA 380

Query: 303 GTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG------- 362
           G DT A+T+EWALS LLNNP+VLKKAR E+D  +G++RLV E DLS L YLQG       
Sbjct: 381 GIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLR 440

Query: 363 ------------------------------------------------------------ 397
                                                                       
Sbjct: 441 LNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG 500

BLAST of Moc02g12370 vs. ExPASy TrEMBL
Match: A0A6J1J8Q1 (cytochrome P450 81D1-like OS=Cucurbita maxima OX=3661 GN=LOC111483619 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 4.1e-117
Identity = 243/497 (48.89%), Postives = 304/497 (61.17%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSEK 62
           M ++LLY+ +   T +LAL F  R  KNLPP+P FSLP+IGHL+L+K P+HRTL  LS K
Sbjct: 1   MAHILLYIFLFPITLLLALKFFPRHGKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLSNK 60

Query: 63  YGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYGE 122
           +G VFSLR GS LVVV+SSPS V ECFT NDIVLANRP  N  KHLTYN T+ + +PYGE
Sbjct: 61  HGHVFSLRFGSRLVVVVSSPSAVHECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 123 HWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTSN 182
            WR LRRIS  EIFS  RL++FL  R++E+KRLLCKL  G+    V VE++ M M+LTSN
Sbjct: 121 LWRTLRRISTREIFSTCRLDLFLGIRQEEVKRLLCKLCRGNT---VVVEVQPMIMDLTSN 180

Query: 183 IVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKRT 242
           IVMRMVAGKR++G+DVSD EQA  FR +VK+VM   G  N  +F+PL NW D + +EK+ 
Sbjct: 181 IVMRMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKI 240

Query: 243 VRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLAG 302
           +++G++AD   QGLIDE RNQK  G N MIDHLL LQ +Q  Y SDQ+IKGLI VILLAG
Sbjct: 241 MKVGEEADAIFQGLIDEIRNQKGSG-NTMIDHLLHLQTTQSEYFSDQIIKGLIHVILLAG 300

Query: 303 TDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-------- 362
            DT AVT+EWALSHLLNNP+V+KKAR E++   G++RLV+E DLS L Y+QG        
Sbjct: 301 IDTTAVTLEWALSHLLNNPEVIKKARFEIERCTGQDRLVNEEDLSSLSYVQGIILETLRL 360

Query: 363 ------------------------------------------------------------ 397
                                                                       
Sbjct: 361 TPAAPLLVPHCASTDCKIQGYDVPRDTIIFVNAWAIHRDPNHWEDVTSFRPERHENAIEL 420

BLAST of Moc02g12370 vs. ExPASy TrEMBL
Match: A0A6J1FSK8 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448412 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 3.4e-116
Identity = 244/497 (49.09%), Postives = 302/497 (60.76%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSEK 62
           M ++LLY+ +   TF+LAL F  R  KNLPP+P FSLP+IGHL+L+K P+HRTL  LS K
Sbjct: 1   MAHILLYIFLFPITFLLALKFFPRHVKNLPPTPLFSLPIIGHLHLLKHPIHRTLHHLSNK 60

Query: 63  YGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYGE 122
           +G VFSLR GS LVVVISSPS V+ECFT NDIVLANRP  N  KHLTYN T+ + +PYGE
Sbjct: 61  HGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 123 HWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTSN 182
            WR LRRIS  EIFS TRL++    R+DE+KRLLCKL    N   V VE++ M M+LTSN
Sbjct: 121 LWRTLRRISTREIFSTTRLDLSSGIRQDEVKRLLCKLCRDDN---VVVEVQPMIMDLTSN 180

Query: 183 IVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKRT 242
           IVMRMVAGKR++G+DVSD EQA  FR +VK+VM   G  N  +F+PL NW D + +EK+ 
Sbjct: 181 IVMRMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKI 240

Query: 243 VRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLAG 302
           +++G++AD    GLIDE RNQK  G N MIDHLL LQ +Q  Y SDQ+IKGLI VILLAG
Sbjct: 241 MKVGEEADAIFNGLIDEIRNQKGSG-NTMIDHLLHLQATQSEYFSDQIIKGLIHVILLAG 300

Query: 303 TDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-------- 362
            DT AVT+EWALSHLLN+P+V+KKAR E++S  G++RLV+E DLS L Y+QG        
Sbjct: 301 IDTTAVTLEWALSHLLNDPEVIKKARFEIESCTGQDRLVNEEDLSSLSYMQGIILETLRL 360

Query: 363 ------------------------------------------------------------ 397
                                                                       
Sbjct: 361 TPAAPLLVPHCASTDCKIQGYDVPRDTIVFVNAWAIHRDPNHWEDVTSFKPERHENAIEL 420

BLAST of Moc02g12370 vs. ExPASy TrEMBL
Match: A0A2I4GDS1 (cytochrome P450 81E8-like OS=Juglans regia OX=51240 GN=LOC109007026 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 1.3e-115
Identity = 251/503 (49.90%), Postives = 306/503 (60.83%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFIL--ALIFLL---RSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQ 62
           ME++LLY  +SL TF+L  AL F     R  KNLPPSPP SLP++GHL+L+K+P+HRT  
Sbjct: 1   MEDILLYSSLSLLTFLLLFALKFYFQTRRLRKNLPPSPPNSLPVLGHLHLLKKPLHRTFY 60

Query: 63  SLSEKYGPVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSA 122
            LS+KYG V SLR GS LVVV+SSPS V+ECFT NDIVLANRP     KHL YN T  +A
Sbjct: 61  RLSQKYGQVLSLRFGSRLVVVVSSPSAVEECFTKNDIVLANRPAMLGGKHLGYNYTTLTA 120

Query: 123 SPYGEHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFM 182
           +PYG+HWR LRRIS LEI S  RLN+F   R+DEIKRLL KL   S   F +VE++S+F 
Sbjct: 121 APYGDHWRNLRRISSLEILSTNRLNMFSGIRRDEIKRLLRKLSHNSCKDFAKVELKSLFS 180

Query: 183 ELTSNIVMRMVAGKRF--YGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDI 242
           ELT NIVMRMVAGK +  YG DV D E+AR+FR I+K+V AN G  N   FVPLL W D 
Sbjct: 181 ELTFNIVMRMVAGKSYYGYGVDVKDEEEARQFREIMKEVTANGGASNPGEFVPLLQWIDR 240

Query: 243 THYEKRTVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLI 302
             +EKR  +L  + D FLQGLIDE+R +KE+G N MIDHLLSLQ+SQ  Y +DQ+IKGLI
Sbjct: 241 GGFEKRLKKLANRTDAFLQGLIDEKRREKEQG-NTMIDHLLSLQKSQPEYYTDQIIKGLI 300

Query: 303 MVILLAGTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG- 362
           +V+LLAGTDT +VT+EWA+S LLN+PD LKKA+ ELD  +G+ERLVDES++S+L YLQ  
Sbjct: 301 LVLLLAGTDTSSVTLEWAMSSLLNHPDTLKKAKVELDGQIGEERLVDESNVSQLCYLQNI 360

Query: 363 ------------------------------------------------------------ 397
                                                                       
Sbjct: 361 ISETLRLYPPAPLLLPHMSSEDCTIEGYDVPRNTIVFINAWAIHRDPNVWDNATDFKPER 420

BLAST of Moc02g12370 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 354.0 bits (907), Expect = 1.6e-97
Identity = 212/500 (42.40%), Postives = 285/500 (57.00%), Query Frame = 0

Query: 1   MEMENLLLYLCVSLTTFILALIFLLRSPK--NLPPSPPFSLPLIGHLYLIKRPVHRTLQS 60
           M++  +L+   +SL  F LA+  L RS +  NLPPSP  SLP+IGHL+L+K P+HRT  S
Sbjct: 1   MDLNQILILSFLSL--FTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLS 60

Query: 61  LSEKYG--PVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFS 120
           LS+  G  PVF LRLG+ LV VISS SI +ECFT ND+VLANRP   I+KHL YN T   
Sbjct: 61  LSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLL 120

Query: 121 ASPYGEHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMF 180
           ++ YG+HWR LRRI+ +EIFS  RLN FL  RKDEI+RL+  L   S H FV VEM+++ 
Sbjct: 121 SASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLL 180

Query: 181 MELTSNIVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDIT 240
             L SN  +RM+AGKR++GED  DA+     + +V + + + G  N  +++ +L W  ++
Sbjct: 181 TNLASNTTIRMLAGKRYFGEDNDDAKLV---KNLVSEAVTSAGAGNPIDYLSILRW--VS 240

Query: 241 HYEKRTVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIM 300
            YEKR   LG + D FLQ L+DE+R +KE+GE  MIDHLL+LQ+ Q +Y +D +IKG+I+
Sbjct: 241 SYEKRIKNLGNRFDTFLQKLVDEKRAEKEKGE-TMIDHLLALQDIQPDYYTDVIIKGIIL 300

Query: 301 VILLAGTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-- 360
            +++AGTDT +VT+EWA+S+LLN+P++LKKAR E+D  VG +RLVDESD+  L YLQ   
Sbjct: 301 TLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIV 360

Query: 361 ------------------------------------------------------------ 397
                                                                       
Sbjct: 361 LETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERF 420

BLAST of Moc02g12370 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 353.6 bits (906), Expect = 2.1e-97
Identity = 202/494 (40.89%), Postives = 281/494 (56.88%), Query Frame = 0

Query: 8   LYLCVSLTTFILALIFLLRSPK---NLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSEKYG 67
           L L  +    +L+LIFL+   K   NLPPSP ++LP+IGHL L+K P+HR   S+S+  G
Sbjct: 4   LMLIFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLG 63

Query: 68  --PVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYGE 127
             P+ SLRLG+ L+ V+SS SI +ECFT ND++LANR  +  TKH++Y N+   ++ Y E
Sbjct: 64  DAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSE 123

Query: 128 HWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTSN 187
           HWR LRRI  LEIFSA RLN F   R+DEI+RL+ +L   S++ F +VEM+SMF +LT N
Sbjct: 124 HWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFN 183

Query: 188 IVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKRT 247
            ++RM+AGK +YG+   D  +A+  R ++ + M++ G  N+ +++P+L W  IT+ E R 
Sbjct: 184 NIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTW--ITYSETRI 243

Query: 248 VRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLAG 307
            +L  + DEFLQGL+DE+R  KE+ EN M+DHLL LQE+Q  Y  D++IKG ++ ++  G
Sbjct: 244 KKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGG 303

Query: 308 TDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG-------- 367
           TDT AVT+EWALS LLNNP+VL KAR E+D ++G +RL++ESD+  L YLQ         
Sbjct: 304 TDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRL 363

Query: 368 ------------------------------------------------------------ 397
                                                                       
Sbjct: 364 YPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEGEA 423

BLAST of Moc02g12370 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 353.6 bits (906), Expect = 2.1e-97
Identity = 208/491 (42.36%), Postives = 286/491 (58.25%), Query Frame = 0

Query: 10  LCVSLTTFILALIFL---LRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQSLSEKY--G 69
           L  S+   +L+LI+L   L+   NLPPSP +SLP+IGHL L+K P+HRT  SLS+     
Sbjct: 6   LIFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNA 65

Query: 70  PVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSASPYGEHW 129
           P+FSLRLG+ LV V SS SI +ECFT ND+VLANRP   + KH+ Y+ T   A+ YG+HW
Sbjct: 66  PIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHW 125

Query: 130 RELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFMELTSNIV 189
           R LRRI  +EIFS  RLN FL  RKDEI+RL+ +L    + +FV+V+M+SM  +LT N +
Sbjct: 126 RNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNI 185

Query: 190 MRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHYEKRTVR 249
           +RMVAGKR+YG+ V D  +A+  R ++  V+A  G  N+ +++P+L    ++ YE R  +
Sbjct: 186 LRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRL--VSDYETRVKK 245

Query: 250 LGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVILLAGTD 309
           L  + DEFLQGL+DE+R  KE+G N MIDHLL+LQESQ +Y +D++IKG ++ ++LAGTD
Sbjct: 246 LAGRLDEFLQGLVDEKREAKEKG-NTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTD 305

Query: 310 TIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQG---------- 369
           T AVT+EWALS++LN+PDVL KAR E+D  +G +RL+DESD+S L YLQ           
Sbjct: 306 TSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYP 365

Query: 370 ------------------------------------------------------------ 396
                                                                       
Sbjct: 366 AAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEGEAQK 425

BLAST of Moc02g12370 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 352.8 bits (904), Expect = 3.5e-97
Identity = 212/504 (42.06%), Postives = 288/504 (57.14%), Query Frame = 0

Query: 1   MEMENL--LLYLCVSLTTFILALIFLLRSPKNLPPSPPFSLPLIGHLYLIKRPVHRTLQS 60
           ME  N+  +LY   SL   I++  FL    +NLPPSPP  LP+IGHL L+K P+HRTL+S
Sbjct: 1   MEETNIRVVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRS 60

Query: 61  LSEKY-----GPVFSLRLGSHLVVVISSPSI-VKECFTVNDIVLANRPLSNITKHLTYNN 120
            SE       G V SLRLGS LV V+SS  +  +ECF  ND+VLANRP   I KH+ YNN
Sbjct: 61  FSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNN 120

Query: 121 TIFSASPYGEHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEM 180
           T   A+PYG+HWR LRR+  +EIFS  RLN FL  R DE++RL+ +L   +  K   VE+
Sbjct: 121 TNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVEL 180

Query: 181 ESMFMELTSNIVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNW 240
           + M M+LT N +MRM+ GKR+YGE+ +D E+A+  R +V  V AN  + N+ ++VP+L  
Sbjct: 181 KPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRL 240

Query: 241 FDITHYEKRTVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIK 300
           F  + YE R  +LG++ D+FLQGLID++R Q+E G   MIDHLL LQ+S + Y +DQ+IK
Sbjct: 241 F--SSYENRVKKLGEETDKFLQGLIDDKRGQQETG-TTMIDHLLVLQKSDIEYYTDQIIK 300

Query: 301 GLIMVILLAGTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYL 360
           G+I+++++AGT+T AVT+EWALS+LLN+PDV+ KAR E+D+ VG +RL++E+DLS+L YL
Sbjct: 301 GIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYL 360

Query: 361 QG---------------------------------------------------------- 397
           +                                                           
Sbjct: 361 KNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFK 420

BLAST of Moc02g12370 vs. TAIR 10
Match: AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7 )

HSP 1 Score: 350.9 bits (899), Expect = 1.3e-96
Identity = 188/355 (52.96%), Postives = 252/355 (70.99%), Query Frame = 0

Query: 3   MENLLLYLCVSLTTFI-LALIFLLRSPK-NLPPSPPFSLPLIGHLYLIKRPVHRTLQSLS 62
           ME   L L ++   FI L L+F  R  K NLPPSP   LP IGHL+L+K+P+HRT  S S
Sbjct: 45  METHFLILSLAFLFFISLKLLFGKRHSKFNLPPSPARPLPFIGHLHLLKQPLHRTFLSFS 104

Query: 63  EKYG--PVFSLRLGSHLVVVISSPSIVKECFTVNDIVLANRPLSNITKHLTYNNTIFSAS 122
           +  G  P+FSLRLG+HL VV+SS SI +ECFT NDIVLANRP   + KH+ YN T  +++
Sbjct: 105 QSLGDAPIFSLRLGNHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTSA 164

Query: 123 PYGEHWRELRRISILEIFSATRLNVFLETRKDEIKRLLCKLRSGSNHKFVRVEMESMFME 182
           PYG+HWR LRRI  LEIFS+ +LN FL  RKDEI+ LL +L   S H F +VEM  +F +
Sbjct: 165 PYGDHWRNLRRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRLSKNSQHGFAKVEMRQLFYD 224

Query: 183 LTSNIVMRMVAGKRFYGEDVSDAEQAREFRGIVKQVMANCGNYNSRNFVPLLNWFDITHY 242
           LT N ++RMVAGKRFYGE     E AR    ++ +++   G  N+ +++P+L W  IT +
Sbjct: 225 LTINNILRMVAGKRFYGEGTEQDEVARRVTQLIDEIVYRAGVGNAADYIPILRW--ITDF 284

Query: 243 EKRTVRLGQQADEFLQGLIDERRNQKEEGENNMIDHLLSLQESQLNYLSDQVIKGLIMVI 302
           EK    L  + DEFLQ L+DERR  K++G N M+DHLLSLQE+Q +Y +D  +KG+I+V+
Sbjct: 285 EKGVKELASRVDEFLQSLVDERRVHKQKG-NTMMDHLLSLQETQPDYYTDVTLKGIIIVM 344

Query: 303 LLAGTDTIAVTIEWALSHLLNNPDVLKKARTELDSVVGKERLVDESDLSKLQYLQ 354
           +LAGT+T+A T+EWA+ +LLN+P+VL+KARTE+D+ VG +RL+DE+D   L YLQ
Sbjct: 345 ILAGTETLAGTLEWAMLNLLNHPEVLEKARTEIDTEVGFDRLMDEADTKNLPYLQ 396