Moc02g09550 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g09550
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Poly(A)-specific ribonuclease
Location	chr2: 6761319 .. 6762212 (+)
RNA-Seq Expression	Moc02g09550
Synteny	Moc02g09550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAGAGGAAAGCGAGGGCATTCGAGAAGAGGAAACGCTCAGATTCGAGAAGTTTGGTCTCGAAATTTCGATTTGGAACTCGCGAATTTGAATGACTGTCTAAATCTCTACCCGATTCTGACCATCGACACAGAATTTCCTGGGTTTATTCGGAGTACTCCATGGGGCGCCATTGAAGAAAAACTCTACGACGATTTTCGATTCAATGTGAATCGGACTAAGCTCATCCAACTGGGTCTCACGGCGTCTGATGAAATCGGCAATATTGGTAGCACATGGGAGTTCAATTTCTCCGATTTCGACCCCCAAATCGACACCCACAACGCCTCCTCTGTCTCCTTCCTGAAACAGAACGGCTTGGATTTTGATAAACTGAAGGAATTCGGAATCCCCATCAACGAATTCACTTCCAAAGCCTTTTTTCTCTTCCGAAAGCATCGGATTGTTCGATGGGTCACGTTCCACGGCTTGTACGACATTGGGTATCTGCTGAAGGCGATGGGGGTGGCCGGGGAGATACCGGAATCAATGGAGGAATTTGCAGAGCTGGTGGAGAGGGCACTGGGGAATGTGAGTGATTTGAAGCATATGGCGCAGTTCTGCGAAGGGCTGGAAGATGGGAAACTTGGGCTTGAGAAACTTGCCCAATTGATGAACCAGAAGCGCTTTGGAAACAAGCACCACGCTGGCTCAGACAGCTTGCTGACGGCTTCGTTGTATGCGAAGATGAAGAATCAATTCGAGCTTGGTTCAGAGGTCTGTGATGGGTTTCTGTATGGCCTCAGGATCAAACTCCATGCGAAGATTAAGCTGTATCAGAATCTGATGTTTGGGCAGATTCCTTATGGCCTTCGAGCTTGTTATCCGCCTTGTTACATTGCTTATTATTGA

mRNA sequence

ATGGGCAGAGGAAAGCGAGGGCATTCGAGAAGAGGAAACGCTCAGATTCGAGAAGTTTGGTCTCGAAATTTCGATTTGGAACTCGCGAATTTGAATGACTGTCTAAATCTCTACCCGATTCTGACCATCGACACAGAATTTCCTGGGTTTATTCGGAGTACTCCATGGGGCGCCATTGAAGAAAAACTCTACGACGATTTTCGATTCAATGTGAATCGGACTAAGCTCATCCAACTGGGTCTCACGGCGTCTGATGAAATCGGCAATATTGGTAGCACATGGGAGTTCAATTTCTCCGATTTCGACCCCCAAATCGACACCCACAACGCCTCCTCTGTCTCCTTCCTGAAACAGAACGGCTTGGATTTTGATAAACTGAAGGAATTCGGAATCCCCATCAACGAATTCACTTCCAAAGCCTTTTTTCTCTTCCGAAAGCATCGGATTGTTCGATGGGTCACGTTCCACGGCTTGTACGACATTGGGTATCTGCTGAAGGCGATGGGGGTGGCCGGGGAGATACCGGAATCAATGGAGGAATTTGCAGAGCTGGTGGAGAGGGCACTGGGGAATGTGAGTGATTTGAAGCATATGGCGCAGTTCTGCGAAGGGCTGGAAGATGGGAAACTTGGGCTTGAGAAACTTGCCCAATTGATGAACCAGAAGCGCTTTGGAAACAAGCACCACGCTGGCTCAGACAGCTTGCTGACGGCTTCGTTGTATGCGAAGATGAAGAATCAATTCGAGCTTGGTTCAGAGGTCTGTGATGGGTTTCTGTATGGCCTCAGGATCAAACTCCATGCGAAGATTAAGCTGTATCAGAATCTGATGTTTGGGCAGATTCCTTATGGCCTTCGAGCTTGTTATCCGCCTTGTTACATTGCTTATTATTGA

Coding sequence (CDS)

ATGGGCAGAGGAAAGCGAGGGCATTCGAGAAGAGGAAACGCTCAGATTCGAGAAGTTTGGTCTCGAAATTTCGATTTGGAACTCGCGAATTTGAATGACTGTCTAAATCTCTACCCGATTCTGACCATCGACACAGAATTTCCTGGGTTTATTCGGAGTACTCCATGGGGCGCCATTGAAGAAAAACTCTACGACGATTTTCGATTCAATGTGAATCGGACTAAGCTCATCCAACTGGGTCTCACGGCGTCTGATGAAATCGGCAATATTGGTAGCACATGGGAGTTCAATTTCTCCGATTTCGACCCCCAAATCGACACCCACAACGCCTCCTCTGTCTCCTTCCTGAAACAGAACGGCTTGGATTTTGATAAACTGAAGGAATTCGGAATCCCCATCAACGAATTCACTTCCAAAGCCTTTTTTCTCTTCCGAAAGCATCGGATTGTTCGATGGGTCACGTTCCACGGCTTGTACGACATTGGGTATCTGCTGAAGGCGATGGGGGTGGCCGGGGAGATACCGGAATCAATGGAGGAATTTGCAGAGCTGGTGGAGAGGGCACTGGGGAATGTGAGTGATTTGAAGCATATGGCGCAGTTCTGCGAAGGGCTGGAAGATGGGAAACTTGGGCTTGAGAAACTTGCCCAATTGATGAACCAGAAGCGCTTTGGAAACAAGCACCACGCTGGCTCAGACAGCTTGCTGACGGCTTCGTTGTATGCGAAGATGAAGAATCAATTCGAGCTTGGTTCAGAGGTCTGTGATGGGTTTCTGTATGGCCTCAGGATCAAACTCCATGCGAAGATTAAGCTGTATCAGAATCTGATGTTTGGGCAGATTCCTTATGGCCTTCGAGCTTGTTATCCGCCTTGTTACATTGCTTATTATTGA

Protein sequence

MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGLRACYPPCYIAYY

Homology

[Close]

BLAST of Moc02g09550 vs. NCBI nr
Match: XP_022146113.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 614.0 bits (1582), Expect = 6.8e-172
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60
           MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE
Sbjct: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60

Query: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120
           EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG
Sbjct: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120

Query: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180
           LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE
Sbjct: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180

Query: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240
           FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL
Sbjct: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240

Query: 241 YAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGLRACYPPCYIAYY 298
           YAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGLRACYPPCYIAYY
Sbjct: 241 YAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGLRACYPPCYIAYY 297

[Close]

BLAST of Moc02g09550 vs. NCBI nr
Match: XP_038895744.1 (putative CCR4-associated factor 1 homolog 8 [Benincasa hispida])

HSP 1 Score: 380.9 bits (977), Expect = 9.7e-102
Identity = 179/297 (60.27%), Postives = 232/297 (78.11%), Query Frame = 0


Query: 7   GHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDD 66
           G +R   + I +VW+ NFD E++ L +CL  + I++IDTEFPGFIR++PWG+I+E LY+D
Sbjct: 2   GENRIRKSLIHKVWASNFDAEISKLEECLEFHTIVSIDTEFPGFIRNSPWGSIDETLYED 61

Query: 67  FRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKL 126
           FRFNVN+TKLIQLGLTA D+ G IG +WEFNFSDFDP+ID  + SS SFL+QNGLDF+KL
Sbjct: 62  FRFNVNQTKLIQLGLTACDDSGKIGGSWEFNFSDFDPEIDAQSPSSASFLEQNGLDFNKL 121

Query: 127 KEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVE 186
           K++GIPIN FT+K   + RKH + RWVTFHGLYDIGYL+KAMG+   +PE+MEEFA +V 
Sbjct: 122 KKYGIPINLFTAKFVHIIRKHNVFRWVTFHGLYDIGYLIKAMGITEVLPETMEEFATVVV 181

Query: 187 RALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKMKN 246
           R LG V DLKHMA+FCEGLEDGKLGLE+L +L++QKR+G KH+AGSDSLLTAS + +M  
Sbjct: 182 RRLGIVRDLKHMARFCEGLEDGKLGLERLGKLLDQKRYGLKHNAGSDSLLTASAHFEMIQ 241

Query: 247 QFELGSEVCDGFLYGLRIKLHA---KIKLYQN--LMFGQIPYGLRA-CYPPCYIAYY 298
           +F++ +E+CDG+LYG   KL +    + ++    + FGQIPY +   CY P YI YY
Sbjct: 242 RFDMDAEICDGYLYGFSNKLQSMDINMNMFHRKIITFGQIPYAIPLYCYVPSYITYY 298

[Close]

BLAST of Moc02g09550 vs. NCBI nr
Match: KAG6604931.1 (putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027010.1 putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 356.7 bits (914), Expect = 2.0e-94
Identity = 177/285 (62.11%), Postives = 217/285 (76.14%), Query Frame = 0


Query: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60
           MGR  R   RR    I+EVW+ NFD E+  L  CL    IL+IDTEFPG IRSTPWG+I+
Sbjct: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120
           E++Y+D RFNVN+TKLIQLGLTASDE G +  +WEFNFSDFD QIDT N SS+SFL+QNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120

Query: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180
           LDF KLK+ GIP++ FT     + R+ R+ +WVTFHGLYDIGYL+KAMGVA E+PESMEE
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240
           FAE+V R +G V DLK +A++CEGLE G++GLE+L +L+ +KRFG KHHAGSDSLLTA +
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 241 YAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGL 286
           +AKM   F L S   DG LYGL+ KL   +K+ Q +MFGQI   L
Sbjct: 241 HAKMARWFRLESGAYDGHLYGLQNKLE-HMKVNQTIMFGQISCSL 284

[Close]

BLAST of Moc02g09550 vs. NCBI nr
Match: XP_023533172.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 354.8 bits (909), Expect = 7.5e-94
Identity = 168/279 (60.22%), Postives = 209/279 (74.91%), Query Frame = 0


Query: 3   RGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEK 62
           RG RG     N  IREVW+ N D E+  L+ CL  Y +++IDTEFPG I+ TPWG I+E+
Sbjct: 6   RGPRG----DNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDEE 65

Query: 63  LYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLD 122
           LY DFRFNVN+TK+IQLG+T SDE+GNIG TWEFNFSDFDP+ID HN +S+ FLKQNGLD
Sbjct: 66  LYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLD 125

Query: 123 FDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFA 182
           F KLK+ GI + +F  +  +  RKH I RWVTFHGLYDIGYL+ AMGV   +PE++ EF 
Sbjct: 126 FGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFE 185

Query: 183 ELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYA 242
            +V R +G V DLKHMA+FCEGLE G LGLEKL QL++QKRFG KHHAGSDSL+TA L+ 
Sbjct: 186 WIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHE 245

Query: 243 KMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQI 282
           KM  +++  +E+CDGFLYGL  K    + L  + ++ QI
Sbjct: 246 KMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQI 280

[Close]

BLAST of Moc02g09550 vs. NCBI nr
Match: KAG7027009.1 (putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 353.6 bits (906), Expect = 1.7e-93
Identity = 167/279 (59.86%), Postives = 209/279 (74.91%), Query Frame = 0


Query: 3   RGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEK 62
           RG RG     N  IREVW+ N D E+  L+ CL  Y +++IDTEFPG I+ TPWG I+E+
Sbjct: 6   RGPRG----DNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEE 65

Query: 63  LYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLD 122
           LY DFRFNVN+TK+IQLG+T SDE+GNIG TWEFNFSDFDP+ID HN +S+ FLKQNGLD
Sbjct: 66  LYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLD 125

Query: 123 FDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFA 182
           F KLK+ GI + +F  +  +  RKH I RW+TFHGLYDIGYL+ AMGV   +PE++ EF 
Sbjct: 126 FGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFE 185

Query: 183 ELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYA 242
            +V R +G V DLKHMA+FCEGLE G LGLEKL QL++QKRFG KHHAGSDSL+TA L+ 
Sbjct: 186 WIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHE 245

Query: 243 KMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQI 282
           KM  +++  +E+CDGFLYGL  K    + L  + ++ QI
Sbjct: 246 KMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQI 280

[Close]

BLAST of Moc02g09550 vs. ExPASy Swiss-Prot
Match: Q9LXM4 (Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=CAF1-8 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 5.3e-50
Identity = 111/222 (50.00%), Postives = 140/222 (63.06%), Query Frame = 0


Query: 31  LNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKLIQLGLTASDEIGNI 90
           + DCL  Y  + IDTEFP  +R T   A +E+ Y D  F+V+R KLIQLGLT  D  G I
Sbjct: 4   IEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRI 63

Query: 91  GSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPINEFTSKAFFLFRK-HRI 150
           G TWE NFSDF    D  N  S+ FL++NGLD  K++E GI I  F S+ F++ +K  R 
Sbjct: 64  GGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRN 123

Query: 151 VRWVTFHGLYDIGYLLKAMGVAGE-IPESMEEFAELVERALGNVSDLKHMAQFCEGLEDG 210
           + WVTFHG YDI YLLK  G  GE +P + E F++ V R LG+V DLK MA  CEGL   
Sbjct: 124 ITWVTFHGSYDIAYLLK--GFTGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SS 183

Query: 211 KLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKMKNQFEL 251
           +LGLE LA      R G  HHAGS++ LTA ++AK+ + F L
Sbjct: 184 RLGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPFPL 221

[Close]

BLAST of Moc02g09550 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.2e-49
Identity = 105/248 (42.34%), Postives = 146/248 (58.87%), Query Frame = 0


Query: 17  REVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKL 76
           R VW  N D E+A + +CL  +P++  DTE+PG I  T + +  ++ Y   + NV  TKL
Sbjct: 10  RRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVENTKL 69

Query: 77  IQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPI--N 136
           IQ G T  +  G IG  WE NFS+F    DT N  S+ FL+++GLD  K+++ G+ +   
Sbjct: 70  IQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVDMFGY 129

Query: 137 EFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERALGNVSD 196
            F  K   +FR  + V +VTF G YD  Y L  +   G++PE+  EFA  V +  G V D
Sbjct: 130 GFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILN-HGKLPETHGEFATEVVKVFGQVYD 189

Query: 197 LKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKMKNQFELGSEV 256
            K MA FCEGL +  LGL KLAQL+   R G  HHAGSDSL+TA ++ K+K+ +E  S  
Sbjct: 190 TKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-DSRF 249

Query: 257 CDGFLYGL 263
             G +YG+
Sbjct: 250 ARGLIYGI 254

[Close]

BLAST of Moc02g09550 vs. ExPASy Swiss-Prot
Match: Q9SFX6 (Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=CAF1-3 PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.2e-47
Identity = 102/229 (44.54%), Postives = 138/229 (60.26%), Query Frame = 0


Query: 18  EVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKLI 77
           EVW  N ++E+ ++ DCL     + IDTEFPG ++ TP  A EE  Y D +FNV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 78  QLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPINE-F 137
           QLG T  D  G    TWE N SDFD      N  S++FLK NGL+ DK++E GI I+E F
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 138 TSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERALGN-VSDL 197
              +  L  K   + WV F G YD  YL+K +     +PE+ EEF E V++ LG  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 198 KHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKM 245
           K +A+ C GL   + GL+++A ++  KR G  HHAGSDS LTA ++ K+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

[Close]

BLAST of Moc02g09550 vs. ExPASy Swiss-Prot
Match: Q9C6M9 (Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=CAF1-4 PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.2e-47
Identity = 103/229 (44.98%), Postives = 138/229 (60.26%), Query Frame = 0


Query: 18  EVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKLI 77
           EVW  N ++E+ ++ DCL  +  + IDTEFPG ++ TP  A EE  Y D +FNV+ T LI
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 78  QLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPINE-F 137
           QLG T  D  G I  TWE N SDF+      N  S++FLK NGL+ DK+ E GI I E F
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 138 TSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERALGN-VSDL 197
              +  L  K   + WV F G YD  YL+K +     +PE+ EEF E VE+ LG  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 198 KHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKM 245
           K +A+ C GL   + GL+++A ++  KR G  HHAGSDS LTA ++ K+
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 229

[Close]

BLAST of Moc02g09550 vs. ExPASy Swiss-Prot
Match: O64773 (Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=CAF1-5 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.2e-47
Identity = 104/229 (45.41%), Postives = 139/229 (60.70%), Query Frame = 0


Query: 18  EVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKLI 77
           EVW  N   E+ ++ DCL     + IDTEFPG ++ TP  A +E  Y D +FNV+ T LI
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 78  QLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPINEFT 137
           QLGLT   +   I  TWE N SDF+      N  S++FLK NGLD DK++E GI I EF 
Sbjct: 64  QLGLTLFGK--GITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 138 SK-AFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERALGN-VSDL 197
            + +  L  KH  +RWVTF G YD  YLLK +     +PE+ +EF E V++ LG  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 198 KHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKM 245
           K MA  C GL   + GL+++A ++  +R G  HHAGSDS LTA ++ K+
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKL 228

[Close]

BLAST of Moc02g09550 vs. ExPASy TrEMBL
Match: A0A6J1CXP9 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015407 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 3.3e-172
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60
           MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE
Sbjct: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60

Query: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120
           EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG
Sbjct: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120

Query: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180
           LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE
Sbjct: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180

Query: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240
           FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL
Sbjct: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240

Query: 241 YAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGLRACYPPCYIAYY 298
           YAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGLRACYPPCYIAYY
Sbjct: 241 YAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGLRACYPPCYIAYY 297

[Close]

BLAST of Moc02g09550 vs. ExPASy TrEMBL
Match: A0A6J1I6H7 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 8.9e-93
Identity = 162/272 (59.56%), Postives = 205/272 (75.37%), Query Frame = 0


Query: 10  RRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRF 69
           R  N  I EVW+ N D E+  L+ CL LYP+++IDTEFPG I+ TPWG I+E+LY DFRF
Sbjct: 9   RGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDEELYADFRF 68

Query: 70  NVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEF 129
           NVN+TK+IQLG+T SDE GNIG TWEFNFSDFDP+ID HN +S+ FLKQNGLDF KLK+ 
Sbjct: 69  NVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKD 128

Query: 130 GIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERAL 189
           GI + +F  +  +  RKH I +W+TFHGLYDIGYL+ A+GV   +PE++ EF  +V R +
Sbjct: 129 GIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEFEWIVARRV 188

Query: 190 GNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKMKNQFE 249
           G V DLKHMA+FCEGLE G LGLEKL QL++QKRFG KHHAGSDSL+TA L+ KM   ++
Sbjct: 189 GTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQLYD 248

Query: 250 LGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQI 282
             +E+CDGFLYGL  K    + +  + ++ QI
Sbjct: 249 FNAEICDGFLYGLSKKFEEFVGMQSHRIYVQI 280

[Close]

BLAST of Moc02g09550 vs. ExPASy TrEMBL
Match: A0A6J1G7L2 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451625 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 1.2e-92
Identity = 170/266 (63.91%), Postives = 207/266 (77.82%), Query Frame = 0


Query: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60
           MGR  R   RR    I+EVW+ NFD E+  L  CL    IL+IDTEFPG IRSTPWG+I+
Sbjct: 1   MGRRNRERPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120
           E++Y+D RFNVN+TKLIQLGLTASDE G +  +WEFNFSDFD QIDT N SS++FL+QNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSIAFLEQNG 120

Query: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180
           LDF KLK+ GIP++ FT     + R+ R+ +WVTFHGLYDIGYL+KAMGVA E+PESMEE
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240
           FAE+V R +G V DLK +A++CEGLE G++GLE+L +L+ +KRFG KHHAGSDSLLTA +
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 241 YAKMKNQFELGSEVCDGFLYGLRIKL 267
           +AKM   F L SE  DG LYGLR KL
Sbjct: 241 HAKMARWFRLESEAYDGHLYGLRNKL 266

[Close]

BLAST of Moc02g09550 vs. ExPASy TrEMBL
Match: A0A6J1I3Q4 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469634 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.6e-92
Identity = 161/272 (59.19%), Postives = 204/272 (75.00%), Query Frame = 0


Query: 10  RRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRF 69
           R  N  I EVW+ N D E+  L+ CL LYP+++IDTEFPG I+ TPWG I+E+LY DFRF
Sbjct: 9   RGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDEELYADFRF 68

Query: 70  NVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEF 129
           NVN+TK+IQLG+T SDE GNIG TWEFNFSDFDP+ID HN +S+ FLKQNGLDF KLK+ 
Sbjct: 69  NVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKD 128

Query: 130 GIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERAL 189
           GI + +F  +  +  RKH I +W+TFHGLYDIGYL+ A+GV   +PE++ EF  +V R +
Sbjct: 129 GIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEFEWIVARRV 188

Query: 190 GNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKMKNQFE 249
           G V DLKHMA+FCEGLE G LGLEKL QL++QKRFG KHHAGSDSL+T  L+ KM   ++
Sbjct: 189 GTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTVLLHEKMLQLYD 248

Query: 250 LGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQI 282
             +E+CDGFLYGL  K    + +  + ++ QI
Sbjct: 249 FNAEICDGFLYGLSKKFEEFVGMQSHRIYVQI 280

[Close]

BLAST of Moc02g09550 vs. ExPASy TrEMBL
Match: A0A6J1G7T4 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.6e-92
Identity = 167/279 (59.86%), Postives = 206/279 (73.84%), Query Frame = 0


Query: 3   RGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEK 62
           RG RG     N  IREVW+ N D E+  L+ CL  Y I++IDTEFPG I+ TPWG  +E+
Sbjct: 6   RGPRG----DNPLIREVWAYNLDHEIIRLDRCLLSYSIMSIDTEFPGCIKRTPWGTFDEE 65

Query: 63  LYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLD 122
           LY DFRFNVN+TK+IQLG+T SDE GNIG TWEFNFSDFDP+ID HN +S+ FLKQNGLD
Sbjct: 66  LYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLD 125

Query: 123 FDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFA 182
           F KLK+ GI + +F  +  +   KH I RWVTFHGLYDIGYL+ AMGV   +PE++ EF 
Sbjct: 126 FGKLKKDGIKVRKFAIRFLYTLGKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFK 185

Query: 183 ELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYA 242
            +V R +G V DLKHMA+FCEGLE G LGLEKL QL++QKRFG KHHAGSDSL+TA L+ 
Sbjct: 186 WIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHE 245

Query: 243 KMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQI 282
           KM  +++  +E+CDGFLYGL  K    + L  + ++ QI
Sbjct: 246 KMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQI 280

[Close]

BLAST of Moc02g09550 vs. TAIR 10
Match: AT3G44240.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 199.5 bits (506), Expect = 3.7e-51
Identity = 111/222 (50.00%), Postives = 140/222 (63.06%), Query Frame = 0


Query: 31  LNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKLIQLGLTASDEIGNI 90
           + DCL  Y  + IDTEFP  +R T   A +E+ Y D  F+V+R KLIQLGLT  D  G I
Sbjct: 4   IEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRI 63

Query: 91  GSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPINEFTSKAFFLFRK-HRI 150
           G TWE NFSDF    D  N  S+ FL++NGLD  K++E GI I  F S+ F++ +K  R 
Sbjct: 64  GGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRN 123

Query: 151 VRWVTFHGLYDIGYLLKAMGVAGE-IPESMEEFAELVERALGNVSDLKHMAQFCEGLEDG 210
           + WVTFHG YDI YLLK  G  GE +P + E F++ V R LG+V DLK MA  CEGL   
Sbjct: 124 ITWVTFHGSYDIAYLLK--GFTGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SS 183

Query: 211 KLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKMKNQFEL 251
           +LGLE LA      R G  HHAGS++ LTA ++AK+ + F L
Sbjct: 184 RLGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPFPL 221

[Close]

BLAST of Moc02g09550 vs. TAIR 10
Match: AT1G06450.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 198.4 bits (503), Expect = 8.3e-51
Identity = 105/248 (42.34%), Postives = 146/248 (58.87%), Query Frame = 0


Query: 17  REVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKL 76
           R VW  N D E+A + +CL  +P++  DTE+PG I  T + +  ++ Y   + NV  TKL
Sbjct: 10  RRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVENTKL 69

Query: 77  IQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPI--N 136
           IQ G T  +  G IG  WE NFS+F    DT N  S+ FL+++GLD  K+++ G+ +   
Sbjct: 70  IQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVDMFGY 129

Query: 137 EFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERALGNVSD 196
            F  K   +FR  + V +VTF G YD  Y L  +   G++PE+  EFA  V +  G V D
Sbjct: 130 GFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILN-HGKLPETHGEFATEVVKVFGQVYD 189

Query: 197 LKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKMKNQFELGSEV 256
            K MA FCEGL +  LGL KLAQL+   R G  HHAGSDSL+TA ++ K+K+ +E  S  
Sbjct: 190 TKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-DSRF 249

Query: 257 CDGFLYGL 263
             G +YG+
Sbjct: 250 ARGLIYGI 254

[Close]

BLAST of Moc02g09550 vs. TAIR 10
Match: AT1G27890.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.0e-48
Identity = 103/229 (44.98%), Postives = 138/229 (60.26%), Query Frame = 0


Query: 18  EVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKLI 77
           EVW  N ++E+ ++ DCL  +  + IDTEFPG ++ TP  A EE  Y D +FNV+ T LI
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 78  QLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPINE-F 137
           QLG T  D  G I  TWE N SDF+      N  S++FLK NGL+ DK+ E GI I E F
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 138 TSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERALGN-VSDL 197
              +  L  K   + WV F G YD  YL+K +     +PE+ EEF E VE+ LG  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 198 KHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKM 245
           K +A+ C GL   + GL+++A ++  KR G  HHAGSDS LTA ++ K+
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 229

[Close]

BLAST of Moc02g09550 vs. TAIR 10
Match: AT1G27820.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.0e-48
Identity = 102/229 (44.54%), Postives = 138/229 (60.26%), Query Frame = 0


Query: 18  EVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKLI 77
           EVW  N ++E+ ++ DCL     + IDTEFPG ++ TP  A EE  Y D +FNV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 78  QLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPINE-F 137
           QLG T  D  G    TWE N SDFD      N  S++FLK NGL+ DK++E GI I+E F
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 138 TSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERALGN-VSDL 197
              +  L  K   + WV F G YD  YL+K +     +PE+ EEF E V++ LG  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 198 KHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKM 245
           K +A+ C GL   + GL+++A ++  KR G  HHAGSDS LTA ++ K+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

[Close]

BLAST of Moc02g09550 vs. TAIR 10
Match: AT1G61470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.0e-48
Identity = 104/229 (45.41%), Postives = 139/229 (60.70%), Query Frame = 0


Query: 18  EVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIEEKLYDDFRFNVNRTKLI 77
           EVW  N   E+ ++ DCL     + IDTEFPG ++ TP  A +E  Y D +FNV+ T LI
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 78  QLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNGLDFDKLKEFGIPINEFT 137
           QLGLT   +   I  TWE N SDF+      N  S++FLK NGLD DK++E GI I EF 
Sbjct: 64  QLGLTLFGK--GITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 138 SK-AFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEEFAELVERALGN-VSDL 197
            + +  L  KH  +RWVTF G YD  YLLK +     +PE+ +EF E V++ LG  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 198 KHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASLYAKM 245
           K MA  C GL   + GL+++A ++  +R G  HHAGSDS LTA ++ K+
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKL 228

The following BLAST results are available for this feature:

BLAST of Moc02g09550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146113.1	6.8e-172	100.00	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_038895744.1	9.7e-102	60.27	putative CCR4-associated factor 1 homolog 8 [Benincasa hispida]	[more]
KAG6604931.1	2.0e-94	62.11	putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp....	[more]
XP_023533172.1	7.5e-94	60.22	putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo]	[more]
KAG7027009.1	1.7e-93	59.86	putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of Moc02g09550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXM4	5.3e-50	50.00	Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SHJ0	1.2e-49	42.34	Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SFX6	4.2e-47	44.54	Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9C6M9	4.2e-47	44.98	Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
O64773	4.2e-47	45.41	Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]

BLAST of Moc02g09550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXP9	3.3e-172	100.00	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015407 PE=...	[more]
A0A6J1I6H7	8.9e-93	59.56	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 S...	[more]
A0A6J1G7L2	1.2e-92	63.91	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451625 PE=3...	[more]
A0A6J1I3Q4	2.6e-92	59.19	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469634 PE=3 S...	[more]
A0A6J1G7T4	2.6e-92	59.86	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3...	[more]

BLAST of Moc02g09550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G44240.1	3.7e-51	50.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G06450.1	8.3e-51	42.34	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G27890.1	3.0e-48	44.98	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G27820.1	3.0e-48	44.54	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G61470.1	3.0e-48	45.41	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 19..132 e-value: 1.0E-6 score: 28.3
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 153..238 e-value: 1.2E-5 score: 24.7
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 6..265 e-value: 1.1E-70 score: 239.8
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 14..270
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 14..270
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 16..252

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g09550.1	Moc02g09550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006402	mRNA catabolic process
biological_process	GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component	GO:0030014	CCR4-NOT complex
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity