Moc02g07490 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g07490
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptiontransmembrane protein 56-B isoform X1
Locationchr2: 5359219 .. 5365438 (+)
RNA-Seq ExpressionMoc02g07490
SyntenyMoc02g07490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGAGGTGTTCAACATGGATTTAAGGTTGATTCATTCCGTCGAAGTAGAAGAAGTTATTCTTGTCTTTTTTTGTTTGTTTTAAGTTTTATTGTTTTCTTTATGAACATTTGAAAATTTACTAATTAAAATAAGATTTATACTTTAATATAAAATATTTTTACCTACTTTTTTTTTGTAATATTTTATTTAAGTCACAATTTATCTGAAAAAGCAAATCTGAGTTCCCGAACAGGCCCACTACGCATTACGAGGCCCAAACTCGCACAATAAGAACGCTCGCAGCGCTCTCGGAACGAAATGCTGACGTGTATTTTCCATTTCTTGTCGGCATCTGTACAGAAAGAGATTATTCCAATTTCCAGTTTCCCACACGGTGGAGAAAGAGCCGATCGACTGGTCAAGTCAAGTATCTGATCTCCTCGTCTGCCATCCACTTGCTCCGCAGTACGTCAAAATTCTCTGTGCTCTACAATATATGTATCTTAATTTACGCCCTCTTTTATTCGATCTCCCGCCGGCCGCCGTGCCGTATTTTCACCTCGCCGGCGGTTTTTTGTTCTGCTACTTCCGCCCGCCGTTGCTACTTTTTTTCCCGTGATTTCAATCAACCGTAATTAAGAAATGTACTTTGATTTCCATAGCCGGCAATGCGGGTTGCTTTCTGAGCCTGTCCTGTCATTTTCGGATAAGTACGTGTATGTCTATGTACAGCATCCGCCGGAAAAAGATGATTTCGAAACTACATTGACGGCATTAACTCATTCGTCACGTGAATTTAGGGCTTTATACTGGTTTGCGTTTACTCCGGTGACGGTGAGTTAGCTGTTCGATATTGATTTGGTTATCTTTTGTTCAGTTTGCAGCAATGGTGCACTTGCCTCTGTCTCTTGCGTACATGAGTATCTCGGTCAGTGACACTCCCGGTCCGAACAAGGAACTTCAATGGCTGGCGTCTATCTTTTCTGGCATTGTTTTCTGCAAGATTGTAAGTGTGCTTATTTGAACTAGTAACGTATCGTATTGTTGATGTTTGATTGAAGTGGATCGAGCGAAAAGTTTTTTCTCTTGACTATTTTCTGCAAGCCGTGATTTCCTCCTGTCTCTCTCTCGCTCTCCATTGATGTATATTGGTTAGTAACCTTCACATCTAGTAAGATCTCTCTGCGTCTTGTTAGACTTGAGAACAGTGTTCTTGCCTCTGTTACGTACGTTCTTTGTTGACCTAGTTTCTCCTGGTTCTTTTAGAAGCTGTCAGTTGATTCAATCACCAATCTCCATTGATTTGTTGTCCTGTCTCTACGCGCGTATATATTAATATAGTTTATTGATGGGAGTATTTTTAGGGCATATATAAAATTTAAGACATGCTACTCTCATATGCAAGTCTGTTGGTTTATTTTGAATTTTTAATTAAGGAAGAAACATATTTCCCCTCTGATTCAGTTACAGTGCATCCACCTTCTTTTGATGTGTGTGAACTTGAAGTTCTAGGGATGTTCTCGACCATTCGCTTCTCCTATACTGGTGTCAAATTTTTCGAAATACGTTTTATTACTTATGTTCATAAATTGCATATACAATATTAAAAAGATAAAAATAACATATAATTTTGAATCTTCTCTTTATTTAAAGAATTGTCAAGTGAAACTTCAATTTTCTTAAAAGATCAAGAACCAGAAAAGCAGAAACAATAATGGTTGTCAAACACATTTTTTTTATTTCTTATTTTGTTCAAAGTAAAAAACAGAGGGAAATAAACAAACGAGAAACGGTTACCAAGTGAGAAACTAGAACTAGGAAATGAAATCAAAGCATTACAAGATGGACCCTTAGTAGCAGTATTTGTGTTGTTGCGTGCTGGCACACGAGTGAAAAGAAGAATGGATGTTAAGGAACTTCTCTGTTACTGGATAATTCACGCTTTCTTTTGTGAAGTATAAATTGGTTTCTTTCTTGAAGGTTTACACATTAACAGGCATTATTAGCTTCCAAAGCTTCAAAGCATATGGCCGGTTAAGCGATTTTGGAAAGGTTGAATGGAACAATAGGTAAACTGATGATTTTGTTTCTTAATGTTGTTTAATAATTAGCTTATTTTTCAGTAATACACTTGCTACGTGAATGTAAAAATATGAATTATTATTCTTTTGTAGGGGGTTTTCAACATTCCACGCCCTTGTTGCAGCATCTTCTTCTCTTTACCTTTTACTATTATCAAGTACTTTTCATCCAGGTTCCAGTGATGAGCTTCTTATAAGAAGAACGTCCTCTTTATCTGATATGACATTGGGGGTAAGCTGAAAATATCGTCAAGGGTTCTTCTTGTGTGATATTTGGTTCTAATTTTTTCTCAACTTTTTTTTTCTTTTTCTTTCCTGTTAGCATTACAATTTTTCTTGTAACCTATTTAGAAGTTGGTTGTTCCAGTTTGCTCCTGGCCACTAAAAAGATGATGAGAATATCATAAGCTAAACTAAACTACCACTTTCATCATCAAGTCATTATTTGATATAAAGGTTCATGGTTTGACACTATGAGTATTGTTCAGAATAGCTACTTGTATTAATTCTTATTGTCGTTCTGTTCTTTCATTGATGAGTAAGTGTAAGATTTTTTGCTCAGACGTGTACATAAGGGAGTTGCAATTGGTCAACCTTGATTAGGTTCAAATACAAGTGCTGATGCATTTAATTATTTTTCATATGGTAGAACTAAAATATCAGCACCGTTGCATTTTAATTATATTCCATATTGATATAGTATAATTTGAAATTTAACATTCAGTAAATATATATATTTTTTTCTTGTAGACACGAAATTTCTAATGCTCTTGCAGTCAACCAAGTGATATGGTAGTAATAAACATAAATGTCATTTTGGTCTCTATCTAGTAATCTACCGATTTTTCTTAATTTCTGGTTCTGTGTTCAATCTTAATTTTTTTTTTCCTTTAATGTGTTCTTATATCTTTTTCCATTATCATCATTTCTCTTCCACTAGGTGCGTAGTGACTGCAAGTTAGTTATGTGAAATGTAATTTTTTTTTACGTATTTAATGTTTTACACTATTCTCCATACAAAAAGAGCAGTTAGTTTTTTCGTTGCCTGTGATTTTTATCGTTTTGAGTTATTAATTTCATTTTTCATTTTCCTGCTGGTCTTTTTACAGTTCTCCATTGGCTATTTTCTATCAGATTTAGCAATGGTTCTTTGGTATTTCCCGGCTTTAGGTGGTCTGGAGTATGTAAGTATGAAGAAAAGGTTCTTTTCTTTTCGTTTATTTTGTTTTTTTTTCGATTACTGGAATATATTAGATTCCATTTGATATCATGTTTAGTTCTGCTTTATGTTTCACTTTTTAATATAGTTTTCTAGAATGGTAAAACAAAATGTTGCTAAATAACTGCTTTTGTTTTTCTTCTTCTTTACTTAATAGAAAGGTAACTAATTTCAGTTGAGAACTGTAATAACAAGAATTTCGTAAGAGAAACCCAAACTTGTTTTCTCAATTTCAGTTTTAGTTTTCATTTTAATAATTAAAAAACGACGTTGATGAAATGCAAGGACATAAACTAGAGACTATTTTTGTGCTTATATTGTAGACATATAAGTAGACTTGTGTGATTCAAAACTGAGTGAAAGCTGTTAACTGATTTTCTTGATTTTAATTGCTTCAATTAGGTCCTACATCATGCGCTATCACTGTTCTCAATCGTTCAGTCCCTCCTAAGTGGTCAAGGACAGATTTACATACTAATGGTTCTTTTTACAGAGAGCACGACTCCATTTGTAAATCTTAGATGGTACAGTTATCGTTAAAAAACTTTACGCTTTCAAATGTTTAATTGTGAACTTTATACTGTGTTTCTAATTAGAAAATAAAGAATTCGTTACTCTGTTTTTCTCATTCAGAAAAAAAAGGTATTTAGTTTTGAACATTGGCTCGTTGCTTTTATTTACTCCATCAGGTACCTGGACAATGCTGGCAACAAGAACTCAAACTTCTACGTCGTCAACGGCATTGCATTGTTTTTCGGGTGGTTGGTAATGTCCATAACTTGAAATCTTGTTTTTTTATAGTGTTTGACAGTAATCCCTAATCAACAGAAATAGTATTTATGTGCATTCATTTAATTGAACTAAGTCCAAGTGTCCAATGGCAACAAAGTTCTTTGATTTTGTACCCCTTGATTTCTTGGTGGAGGATTATTTTCCTCATGGAAAAGCTATAAATTTTGAAAAGGTAGGGGGCAATTTTATTTTTCTAATGTTTCTGATGCGATGTTATCATTTCCATGGAATTTAGACGTAATTAATACGAGTTCCATCTATTTCATTTCCATTTTTTTATTCAAGACTGACATGGGTTTTGTTTCTTCAGGTTGCCAGGATTCTCTTGTTTATATATTTTTTTAGTCACATGTATAGAAATTTTGATGAGGTAAGTGATTATCATCTCTCGTTGCTAACTGAGCCTTTGAATATTTTGCTTATGGAAAATTATTTTCTCTGTCAACTGTGAAGAGAATAGAATTAAACCACAAAAGGATGAATAAATTCCTATATGGTAACATTATCCAATTACAGCTTCCTAAAAGTAGGATGCTAGAAATTATAGATTTACAAGCTTGATTTATTCAAGAGAAGCTAGAGATGTATGTATAAACTGACACCCAAGGATTTCATTCTTTCGACGAAATACTAGTTAGGCTGTTCGTTATCGAAAAGAAAAATGTAATATCTGTAGCAGTTTGATGCCGACTGTTTGAACACTTCTGTCTATAATCCAATAGGACCCGGATCTCGAAAATAGAAGAAAACAACACTAAATTCTATTATGATATGGAAGGGAAACTTACAATTCTCGAACATTTAAGAGGCTGAAACTCTTTCATAATAAGCCAACAAAAGAGGTTAGTATGTATCATCGACTAAAAGGTCGGAGGTTTGAATCCCTACTTGAACCCAACAAGAAAAGATAAAAAGAAAAACTCTTCCATAATAAATACCTTTTAAATCTTGCCTACCCCCAAACTTTCCTAGTACTCCTATTTATAACCACCTTTTGCCAGCCAACTCCTACCAATGATAAATATGCTTTCTAGTATCCTATTCCTAATACTGTCTCTAATATTTCCTACTAATAGTCCTAACACCCTACGACTAAAAGCATCTTTTCAAGAAAAAGGAAAACAAACTACCTTCAAATGGTTCCTCTTAATCACAGTGAATGAATAAATACTCCAAATTTCATGTTTCAAGGTAGTTTGTTTTCTCGGTGGCTCGAGTAGGATTCCTGAAGAAATATTTGCCATGTTTCTCTGAAGTGGGTAGGTAGGAGATATTGGTAAAACTTTTTAGGCTAAATTACAAAAAATACCCGTGAGTTTTTCCATTTGTTTAAATAATACCTCCATCCTTTCAAAAATTTCAACACTTCCCTAACCTTTCATCAACATTTAAAAAAATATCCTTTGACCAAACATCCATTAATATTTTGAACAAAAAGTTAACATGACAAGAATCTGAAACGATGTGACAATTAACTTGGAAGCTTGCCTAGAACTATAAGATGGACGAAGTCATTCAACCACGTTATTTTTGACGAAGAGAAGAGGGAGGAAAAGATTTGCCATTCAGTCACGTTAAATGCCCTAACCCCTAAATTTCAAAACGACGTCATCCACCTAATTGTTCTAGGCAAACTTCTATGTTAATTGTTACGTTGTTCTAGATTCTGTCACGTCAACTTTTTTTTGTCCAAAATATTAACAAATGTTTGCTCAAATGTAACTTTGAAAGGTTTATAAAAGGTAAAGGTAGTAGAACTTCGAAAGGATAAGAGTATTTTTTAGAGGAAGGCAAAATTTAGGAGTATTTACTTTTTTTCCTTTGTTGTTGTTTTTTTTTTTTGTTTGGGGTAATTTGGCCCACTTATTACAATTCGTTAATGGCCGACTATTTTGTTGAAGTGGGTAGATTGGAAATATTGGTAAAGCCTTTAAGCACTCCCAAAGAAATTGAATACTGCCAGAATTAGGCGGTGGCTGCTGAATTTTCTGTTTCATGTGTTTTAGGTGAAGACTGTGTTTCCTTTGGGCTTCTATAGCTTGCTGGCGGTGCCTCCTGTTCTTTCTATGATGAATCTGTTCTGGTTTTGGAAGATCGCCAGAGGAATGATTAAAACTATCAGAAAGGCAAGGCACGGCAAATGA

mRNA sequence

ATGAATGAGGTGTTCAACATGGATTTAAGAAAGAGATTATTCCAATTTCCAGTTTCCCACACGGTGGAGAAAGAGCCGATCGACTGGTCAAGTCAAGTATCTGATCTCCTCGTCTGCCATCCACTTGCTCCGCACCGGCAATGCGGGTTGCTTTCTGAGCCTGTCCTGTCATTTTCGGATAAGTACGTGTATGTCTATGTACAGCATCCGCCGGAAAAAGATGATTTCGAAACTACATTGACGGCATTAACTCATTCGTCACCAATGGTGCACTTGCCTCTGTCTCTTGCGTACATGAGTATCTCGGTCAGTGACACTCCCGGTCCGAACAAGGAACTTCAATGGCTGGCGTCTATCTTTTCTGGCATTGTTTTCTGCAAGATTGTTTACACATTAACAGGCATTATTAGCTTCCAAAGCTTCAAAGCATATGGCCGGTTAAGCGATTTTGGAAAGGTTGAATGGAACAATAGGGGGTTTTCAACATTCCACGCCCTTGTTGCAGCATCTTCTTCTCTTTACCTTTTACTATTATCAAGTACTTTTCATCCAGGTTCCAGTGATGAGCTTCTTATAAGAAGAACGTCCTCTTTATCTGATATGACATTGGGGTTCTCCATTGGCTATTTTCTATCAGATTTAGCAATGGTTCTTTGGTATTTCCCGGCTTTAGGTGGTCTGGAGTATGTCCTACATCATGCGCTATCACTGTTCTCAATCGTTCAGTCCCTCCTAAGTGGTCAAGGACAGATTTACATACTAATGGTTCTTTTTACAGAGAGCACGACTCCATTTGTAAATCTTAGATGGTACCTGGACAATGCTGGCAACAAGAACTCAAACTTCTACGTCGTCAACGGCATTGCATTGTTTTTCGGGTGGTTGGTTGCCAGGATTCTCTTGTTTATATATTTTTTTAGTCACATGTATAGAAATTTTGATGAGGTGAAGACTGTGTTTCCTTTGGGCTTCTATAGCTTGCTGGCGGTGCCTCCTGTTCTTTCTATGATGAATCTGTTCTGGTTTTGGAAGATCGCCAGAGGAATGATTAAAACTATCAGAAAGGCAAGGCACGGCAAATGA

Coding sequence (CDS)

ATGAATGAGGTGTTCAACATGGATTTAAGAAAGAGATTATTCCAATTTCCAGTTTCCCACACGGTGGAGAAAGAGCCGATCGACTGGTCAAGTCAAGTATCTGATCTCCTCGTCTGCCATCCACTTGCTCCGCACCGGCAATGCGGGTTGCTTTCTGAGCCTGTCCTGTCATTTTCGGATAAGTACGTGTATGTCTATGTACAGCATCCGCCGGAAAAAGATGATTTCGAAACTACATTGACGGCATTAACTCATTCGTCACCAATGGTGCACTTGCCTCTGTCTCTTGCGTACATGAGTATCTCGGTCAGTGACACTCCCGGTCCGAACAAGGAACTTCAATGGCTGGCGTCTATCTTTTCTGGCATTGTTTTCTGCAAGATTGTTTACACATTAACAGGCATTATTAGCTTCCAAAGCTTCAAAGCATATGGCCGGTTAAGCGATTTTGGAAAGGTTGAATGGAACAATAGGGGGTTTTCAACATTCCACGCCCTTGTTGCAGCATCTTCTTCTCTTTACCTTTTACTATTATCAAGTACTTTTCATCCAGGTTCCAGTGATGAGCTTCTTATAAGAAGAACGTCCTCTTTATCTGATATGACATTGGGGTTCTCCATTGGCTATTTTCTATCAGATTTAGCAATGGTTCTTTGGTATTTCCCGGCTTTAGGTGGTCTGGAGTATGTCCTACATCATGCGCTATCACTGTTCTCAATCGTTCAGTCCCTCCTAAGTGGTCAAGGACAGATTTACATACTAATGGTTCTTTTTACAGAGAGCACGACTCCATTTGTAAATCTTAGATGGTACCTGGACAATGCTGGCAACAAGAACTCAAACTTCTACGTCGTCAACGGCATTGCATTGTTTTTCGGGTGGTTGGTTGCCAGGATTCTCTTGTTTATATATTTTTTTAGTCACATGTATAGAAATTTTGATGAGGTGAAGACTGTGTTTCCTTTGGGCTTCTATAGCTTGCTGGCGGTGCCTCCTGTTCTTTCTATGATGAATCTGTTCTGGTTTTGGAAGATCGCCAGAGGAATGATTAAAACTATCAGAAAGGCAAGGCACGGCAAATGA

Protein sequence

MNEVFNMDLRKRLFQFPVSHTVEKEPIDWSSQVSDLLVCHPLAPHRQCGLLSEPVLSFSDKYVYVYVQHPPEKDDFETTLTALTHSSPMVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGMIKTIRKARHGK
Homology
BLAST of Moc02g07490 vs. NCBI nr
Match: XP_022149739.1 (transmembrane protein 56-B isoform X1 [Momordica charantia] >XP_022149740.1 transmembrane protein 56-B isoform X1 [Momordica charantia])

HSP 1 Score: 538.1 bits (1385), Expect = 5.8e-149
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 89  MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 148
           MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS
Sbjct: 1   MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 60

Query: 149 DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 208
           DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG
Sbjct: 61  DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 120

Query: 209 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 268
           YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL
Sbjct: 121 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 180

Query: 269 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 328
           RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL
Sbjct: 181 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 240

Query: 329 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
           AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK
Sbjct: 241 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 272

BLAST of Moc02g07490 vs. NCBI nr
Match: XP_023533878.1 (transmembrane protein 56-B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.8 bits (1231), Expect = 4.2e-131
Identity = 233/272 (85.66%), Postives = 260/272 (95.59%), Query Frame = 0

Query: 89  MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 148
           +VHLPLSLAYM+ SV+D   P+K LQWLAS+FSGIVFCKIVYTLTGI+S QSFKAYG+LS
Sbjct: 13  IVHLPLSLAYMTFSVTDASDPSKGLQWLASVFSGIVFCKIVYTLTGILSLQSFKAYGKLS 72

Query: 149 DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 208
           DFGKVEWNNRGFSTFHALVAASSSLYL+LLSSTF+PGSS+ELLIRRTSSLSDMTLGFSIG
Sbjct: 73  DFGKVEWNNRGFSTFHALVAASSSLYLVLLSSTFNPGSSNELLIRRTSSLSDMTLGFSIG 132

Query: 209 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 268
           YFLSDLAMVLW+FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNL
Sbjct: 133 YFLSDLAMVLWFFPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTEITTPFVNL 192

Query: 269 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 328
           RWYLDNAG KNSN YV+NGIALFFGWLVARI+LFIYFF HM+++FDEVK+V+PLGFY+LL
Sbjct: 193 RWYLDNAGQKNSNLYVINGIALFFGWLVARIVLFIYFFGHMFKHFDEVKSVYPLGFYTLL 252

Query: 329 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
           AVPP+L++MN+FWFWKIARGMIKT++KARH K
Sbjct: 253 AVPPMLAIMNIFWFWKIARGMIKTLKKARHSK 284

BLAST of Moc02g07490 vs. NCBI nr
Match: XP_023007223.1 (transmembrane protein 56-B-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 478.0 bits (1229), Expect = 7.1e-131
Identity = 233/272 (85.66%), Postives = 260/272 (95.59%), Query Frame = 0

Query: 89  MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 148
           +VHLPLSLAYM+ SV+D   P+K LQWLAS+FSGIVFCKIVYTLTGI+S QSFKAYG+LS
Sbjct: 13  IVHLPLSLAYMTFSVTDASDPSKGLQWLASVFSGIVFCKIVYTLTGILSLQSFKAYGQLS 72

Query: 149 DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 208
           DFGKVEWNNRGFSTFHALVAASSSLYL+LLSSTF+PGSS+ELLIRRTSSLSDMTLGFSIG
Sbjct: 73  DFGKVEWNNRGFSTFHALVAASSSLYLVLLSSTFNPGSSNELLIRRTSSLSDMTLGFSIG 132

Query: 209 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 268
           YFLSDLAMVLW+FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNL
Sbjct: 133 YFLSDLAMVLWFFPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTEITTPFVNL 192

Query: 269 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 328
           RWYLDNAG KNSN YV+NGIALFFGWLVARI+LFIYFF HM+++FDEVK+V+PLGFY+LL
Sbjct: 193 RWYLDNAGQKNSNLYVINGIALFFGWLVARIVLFIYFFGHMFKHFDEVKSVYPLGFYTLL 252

Query: 329 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
           AVPP+L++MN+FWFWKIARGMIKT++KARH K
Sbjct: 253 AVPPMLAVMNVFWFWKIARGMIKTLKKARHSK 284

BLAST of Moc02g07490 vs. NCBI nr
Match: XP_022948078.1 (transmembrane protein 56-B-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 477.2 bits (1227), Expect = 1.2e-130
Identity = 232/272 (85.29%), Postives = 260/272 (95.59%), Query Frame = 0

Query: 89  MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 148
           +VHLPLSLAYM+ SV+D   P+K LQW+AS+FSGIVFCKIVYTLTGI+S QSFKAYG+LS
Sbjct: 13  IVHLPLSLAYMTFSVTDASDPSKGLQWMASVFSGIVFCKIVYTLTGILSLQSFKAYGKLS 72

Query: 149 DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 208
           DFGKVEWNNRGFSTFHALVAASSSLYL+LLSSTF+PGSS+ELLIRRTSSLSDMTLGFSIG
Sbjct: 73  DFGKVEWNNRGFSTFHALVAASSSLYLVLLSSTFNPGSSNELLIRRTSSLSDMTLGFSIG 132

Query: 209 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 268
           YFLSDLAMVLW+FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNL
Sbjct: 133 YFLSDLAMVLWFFPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTEITTPFVNL 192

Query: 269 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 328
           RWYLDNAG KNSNFYV+NGIALFFGWLVARI+LFIYFF HM+++FDEVK+V+ LGFY+LL
Sbjct: 193 RWYLDNAGQKNSNFYVINGIALFFGWLVARIVLFIYFFGHMFKHFDEVKSVYSLGFYTLL 252

Query: 329 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
           AVPP+L++MN+FWFWKIARGMIKT++KARH K
Sbjct: 253 AVPPMLAIMNIFWFWKIARGMIKTLKKARHSK 284

BLAST of Moc02g07490 vs. NCBI nr
Match: KAG6570610.1 (TLC domain-containing protein 4-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 476.5 bits (1225), Expect = 2.1e-130
Identity = 233/272 (85.66%), Postives = 256/272 (94.12%), Query Frame = 0

Query: 89  MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 148
           +VHL LSLAYM+ISV+D  GP KELQWLAS+FSGIVFCKIVYTLTGI+S QSFK YG+LS
Sbjct: 36  IVHLLLSLAYMTISVNDVSGPGKELQWLASVFSGIVFCKIVYTLTGIVSLQSFKTYGKLS 95

Query: 149 DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 208
           DFGKVEWNNRGFSTFHALVAASSSLYL+LLSSTF PGSS+ELLIRR+SSLSDMTLGFS+G
Sbjct: 96  DFGKVEWNNRGFSTFHALVAASSSLYLVLLSSTFDPGSSNELLIRRSSSLSDMTLGFSVG 155

Query: 209 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 268
           YFLSDLAMVLWYFPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNL
Sbjct: 156 YFLSDLAMVLWYFPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTECTTPFVNL 215

Query: 269 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 328
           RWYLDNAG KNSN YV+NGIALF GWLVARILLFIYFF HM+++FD+VKTV+PLG YSLL
Sbjct: 216 RWYLDNAGQKNSNSYVINGIALFLGWLVARILLFIYFFGHMFKHFDQVKTVYPLGLYSLL 275

Query: 329 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
            VPP+L+MMN+FWFWKI+RGMIKT+RKARH K
Sbjct: 276 TVPPMLAMMNVFWFWKISRGMIKTLRKARHNK 307

BLAST of Moc02g07490 vs. ExPASy Swiss-Prot
Match: Q6PGS5 (TLC domain-containing protein 4-B OS=Xenopus laevis OX=8355 GN=tlcd4-b PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.6e-16
Identity = 67/241 (27.80%), Postives = 118/241 (48.96%), Query Frame = 0

Query: 118 SIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAASSSLYLLL 177
           S+ S  VF  + +  +  +S +    + +LS   K+EWN+R  S+FHALV     LY+L+
Sbjct: 12  SVTSLAVFQFLFHVGSSWLSTRLTGGFHKLSARQKIEWNSRTVSSFHALVVGCFCLYILV 71

Query: 178 LSSTFH--PGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHAL 237
                +  P   D  +++       + +  + GY +SDL ++++Y+  +G   +V HH  
Sbjct: 72  YDDAVNADPVWGDPFMVK-------LNVAVTSGYLISDLLLIIYYWKEIGDKYFVTHHLA 131

Query: 238 SLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAG-NKNSNFYVVNGIALFFGW 297
           +L++    L  G    +    L  E +TPFVN RW+ +  G +K S   +VNG+ +   +
Sbjct: 132 ALYACYYVLGEGMLPYFGNFRLIAEFSTPFVNQRWFFEVLGYSKYSLPNMVNGVLMTISF 191

Query: 298 LVARILLFIYFFSHMYRNF-DEVKTVFPLGFYSLLAVPPV-LSMMNLFWFWKIARGMIKT 354
            + RI +   ++  ++  F  E      LG      +  V L +MN+ W  KIA+G  K 
Sbjct: 192 FIVRIAVIPIYYGRVFSTFGTEAFHRLGLGAQCAWIISSVSLDIMNVMWMIKIAKGCYKV 245

BLAST of Moc02g07490 vs. ExPASy Swiss-Prot
Match: Q96MV1 (TLC domain-containing protein 4 OS=Homo sapiens OX=9606 GN=TLCD4 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 3.0e-15
Identity = 72/255 (28.24%), Postives = 119/255 (46.67%), Query Frame = 0

Query: 110 NKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAA 169
           N +L    +  S   F  + Y ++   S +    +  LS   K+EWN+R  ST H+LV  
Sbjct: 4   NTKLLISVTCISFFTFQLLFYFVSYWFSAKVSPGFNSLSFKKKIEWNSRVVSTCHSLVVG 63

Query: 170 SSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEY 229
              LY+ L         +D L      SL+++ +  + GY +SDL++++ Y+  +G   +
Sbjct: 64  IFGLYIFLFDEA---TKADPLW--GGPSLANVNIAIASGYLISDLSIIILYWKVIGDKFF 123

Query: 230 VLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAG-NKNSNFYVVNGI 289
           ++HH  SL++    L +G         L  E ++PFVN RW+ +     K S   V+NGI
Sbjct: 124 IMHHCASLYAYYLVLKNGVLAYIGNFRLLAELSSPFVNQRWFFEALKYPKFSKAIVINGI 183

Query: 290 ALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGF---YSLLAVPPVLSMMNLFWFWKI 349
            +   + + RI   +  +  MY  +   +    LG     S +    VL +MN+ W  KI
Sbjct: 184 LMTVVFFIVRIASMLPHYGFMYSVYG-TEPYIRLGVLIQLSWVISCVVLDVMNVMWMIKI 243

Query: 350 ARGMIKTIRKARHGK 361
           ++G IK I   R  K
Sbjct: 244 SKGCIKVISHIRQEK 252

BLAST of Moc02g07490 vs. ExPASy Swiss-Prot
Match: Q6P4N1 (TLC domain-containing protein 4 OS=Xenopus tropicalis OX=8364 GN=tlcd4 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.9e-15
Identity = 71/239 (29.71%), Postives = 113/239 (47.28%), Query Frame = 0

Query: 132 LTGIISFQSFKAYG---RLSDFGK-VEWNNRGFSTFHALVAASSSLYLLLLSSTFH--PG 191
           L  IIS ++F  Y    R   FGK  EW++R  ST HALV  S  LY+L      +  P 
Sbjct: 22  LFSIISPRTFTKYSSTYRQLSFGKQCEWDSRCVSTTHALVVGSGCLYILAYDEAVNADPI 81

Query: 192 SSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLL 251
             D   ++       M +  + GY + DL ++  ++  +     V HH    +S    L 
Sbjct: 82  WGDPFWVK-------MNVAITCGYLVHDLLLLARFWKVMRDPYMVCHHLAVFYSYGYVLN 141

Query: 252 SGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYV-VNGIALFFGWLVARILLFIY 311
            G    +    L +E +TPFVN RW+ D  G   S++ V +NG+A+   + + RI +   
Sbjct: 142 RGVLPYFANFRLISELSTPFVNQRWFFDVIGKPRSSWPVLLNGLAMALVFFIVRIAVIPS 201

Query: 312 FFSHMYRNFDEVKTVFPLGF---YSLLAVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
           ++S ++  F   +    LG     + +    VL ++N+FW +KIARG  K ++    GK
Sbjct: 202 YYSQVFATFG-TEGYIRLGIGPQVAWIVSCVVLDILNVFWMYKIARGFYKVVKAKPDGK 252

BLAST of Moc02g07490 vs. ExPASy Swiss-Prot
Match: Q6GLX2 (TLC domain-containing protein 4-A OS=Xenopus laevis OX=8355 GN=tlcd4-a PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.1e-14
Identity = 65/230 (28.26%), Postives = 109/230 (47.39%), Query Frame = 0

Query: 138 FQSFKAYGRLSDFGK-VEWNNRGFSTFHALVAASSSLYLLLLSSTFH--PGSSDELLIRR 197
           F  + +  R   FGK  EW++R  ST HAL+  S+ LY+L      +  P   D   ++ 
Sbjct: 30  FTKYSSTYRQLSFGKQCEWDSRFVSTNHALIVGSACLYILAYDDAVNADPIWGDPFWVK- 89

Query: 198 TSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYIL 257
                 M +  + GY + DL ++  ++  +     V HH    +S    L  G    +  
Sbjct: 90  ------MNVAITCGYLVQDLLLLARFWKVMRDPYMVCHHLAVFYSYGYVLNRGVLPYFAN 149

Query: 258 MVLFTESTTPFVNLRWYLDNAGNKNSNFYV-VNGIALFFGWLVARILLFIYFFSHMYRNF 317
             L +E +TPFVN RW+ D  G   S++ V +NG+A+   + + RI +   ++S ++  F
Sbjct: 150 FRLISELSTPFVNQRWFFDVIGKPRSSWPVLLNGLAMALVFFIVRIAVIPSYYSQVFATF 209

Query: 318 DEVKTVFPLGF---YSLLAVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
              +    LG     + +    VL ++N+FW +KIARG  K ++    GK
Sbjct: 210 G-TEGYIRLGIGPQVAWIVSCVVLDILNVFWMYKIARGFYKVVKAKPDGK 251

BLAST of Moc02g07490 vs. ExPASy Swiss-Prot
Match: Q550S9 (TLC domain-containing protein 4 B OS=Dictyostelium discoideum OX=44689 GN=tlcd4b PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.6e-14
Identity = 60/207 (28.99%), Postives = 107/207 (51.69%), Query Frame = 0

Query: 152 KVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFL 211
           ++EW NR  ST +A+V ++ S+Y L  +            +R TS +S     F   YF+
Sbjct: 46  RIEWPNRIISTVNAIVTSALSIYCLYYNEWIVNS------LRSTSEMSYFIFKFITYYFI 105

Query: 212 SDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIY--ILMVLFTESTTPFVNLR 271
            D  +  +Y   L     +LHH ++L S   + L G+G  +  +L   FTE TTP +NLR
Sbjct: 106 YDFIISSYYSKYLFTWGNLLHHTIALLSF--TFLGGKGLAHHLLLSYTFTEITTPLINLR 165

Query: 272 WYLDNAGNKNSNFYVVNGIALFFGWLVARIL-----LFIYFFSHMYRNFDEVKTVFPLGF 331
           ++L +   KN   YV+NG+ +F G+++ R+      +F   F+  + + +      PL  
Sbjct: 166 FFLLDLNLKNHPLYVINGLLIFVGFVLFRVFYTSATMFDVIFNQPHYSIE----TDPLIP 225

Query: 332 YSLLAVPPVLSMMNLFWFWKIARGMIK 352
           + +  V P ++++NL+W + I++ + K
Sbjct: 226 FFINFVYPAITLLNLYWTFYISKSIFK 240

BLAST of Moc02g07490 vs. ExPASy TrEMBL
Match: A0A6J1D7X6 (transmembrane protein 56-B isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018098 PE=4 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.8e-149
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 89  MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 148
           MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS
Sbjct: 1   MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 60

Query: 149 DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 208
           DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG
Sbjct: 61  DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 120

Query: 209 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 268
           YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL
Sbjct: 121 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 180

Query: 269 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 328
           RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL
Sbjct: 181 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 240

Query: 329 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
           AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK
Sbjct: 241 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 272

BLAST of Moc02g07490 vs. ExPASy TrEMBL
Match: A0A6J1L744 (transmembrane protein 56-B-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499774 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 3.4e-131
Identity = 233/272 (85.66%), Postives = 260/272 (95.59%), Query Frame = 0

Query: 89  MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 148
           +VHLPLSLAYM+ SV+D   P+K LQWLAS+FSGIVFCKIVYTLTGI+S QSFKAYG+LS
Sbjct: 13  IVHLPLSLAYMTFSVTDASDPSKGLQWLASVFSGIVFCKIVYTLTGILSLQSFKAYGQLS 72

Query: 149 DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 208
           DFGKVEWNNRGFSTFHALVAASSSLYL+LLSSTF+PGSS+ELLIRRTSSLSDMTLGFSIG
Sbjct: 73  DFGKVEWNNRGFSTFHALVAASSSLYLVLLSSTFNPGSSNELLIRRTSSLSDMTLGFSIG 132

Query: 209 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 268
           YFLSDLAMVLW+FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNL
Sbjct: 133 YFLSDLAMVLWFFPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTEITTPFVNL 192

Query: 269 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 328
           RWYLDNAG KNSN YV+NGIALFFGWLVARI+LFIYFF HM+++FDEVK+V+PLGFY+LL
Sbjct: 193 RWYLDNAGQKNSNLYVINGIALFFGWLVARIVLFIYFFGHMFKHFDEVKSVYPLGFYTLL 252

Query: 329 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
           AVPP+L++MN+FWFWKIARGMIKT++KARH K
Sbjct: 253 AVPPMLAVMNVFWFWKIARGMIKTLKKARHSK 284

BLAST of Moc02g07490 vs. ExPASy TrEMBL
Match: A0A6J1G8D2 (transmembrane protein 56-B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451771 PE=4 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.8e-131
Identity = 232/272 (85.29%), Postives = 260/272 (95.59%), Query Frame = 0

Query: 89  MVHLPLSLAYMSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLS 148
           +VHLPLSLAYM+ SV+D   P+K LQW+AS+FSGIVFCKIVYTLTGI+S QSFKAYG+LS
Sbjct: 13  IVHLPLSLAYMTFSVTDASDPSKGLQWMASVFSGIVFCKIVYTLTGILSLQSFKAYGKLS 72

Query: 149 DFGKVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIG 208
           DFGKVEWNNRGFSTFHALVAASSSLYL+LLSSTF+PGSS+ELLIRRTSSLSDMTLGFSIG
Sbjct: 73  DFGKVEWNNRGFSTFHALVAASSSLYLVLLSSTFNPGSSNELLIRRTSSLSDMTLGFSIG 132

Query: 209 YFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNL 268
           YFLSDLAMVLW+FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNL
Sbjct: 133 YFLSDLAMVLWFFPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTEITTPFVNL 192

Query: 269 RWYLDNAGNKNSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLL 328
           RWYLDNAG KNSNFYV+NGIALFFGWLVARI+LFIYFF HM+++FDEVK+V+ LGFY+LL
Sbjct: 193 RWYLDNAGQKNSNFYVINGIALFFGWLVARIVLFIYFFGHMFKHFDEVKSVYSLGFYTLL 252

Query: 329 AVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 361
           AVPP+L++MN+FWFWKIARGMIKT++KARH K
Sbjct: 253 AVPPMLAIMNIFWFWKIARGMIKTLKKARHSK 284

BLAST of Moc02g07490 vs. ExPASy TrEMBL
Match: A0A6J1JHF4 (transmembrane protein 56-B-like OS=Cucurbita maxima OX=3661 GN=LOC111484516 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 8.7e-127
Identity = 226/262 (86.26%), Postives = 247/262 (94.27%), Query Frame = 0

Query: 99  MSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNR 158
           M+ISV+D  GP+KELQWLAS+FSGIVFCKIVYTLTGI+S QSFK YG+LSDFGKVEW NR
Sbjct: 1   MTISVTDASGPSKELQWLASVFSGIVFCKIVYTLTGIVSLQSFKTYGKLSDFGKVEWKNR 60

Query: 159 GFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVL 218
           GFSTFHALVAASSSLYL+LLSSTF PGSS+ELLIRRTSSLSD TLGFS+GYFLSDLAMVL
Sbjct: 61  GFSTFHALVAASSSLYLVLLSSTFDPGSSNELLIRRTSSLSDTTLGFSVGYFLSDLAMVL 120

Query: 219 WYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNK 278
           WYFPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG K
Sbjct: 121 WYFPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTECTTPFVNLRWYLDNAGQK 180

Query: 279 NSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMN 338
           NSN YV+NGIALF GWLVARILLFIYFF HM+++FD+VKTV+PLGFYSLL VPP+L+MMN
Sbjct: 181 NSNSYVINGIALFLGWLVARILLFIYFFGHMFKHFDQVKTVYPLGFYSLLTVPPMLAMMN 240

Query: 339 LFWFWKIARGMIKTIRKARHGK 361
           +FWFWKIARGMIKT+RKARH K
Sbjct: 241 VFWFWKIARGMIKTLRKARHNK 262

BLAST of Moc02g07490 vs. ExPASy TrEMBL
Match: A0A6J1FYI8 (transmembrane protein 56-B OS=Cucurbita moschata OX=3662 GN=LOC111448719 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.9e-126
Identity = 226/262 (86.26%), Postives = 247/262 (94.27%), Query Frame = 0

Query: 99  MSISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNR 158
           M+ISV+D   P+KELQWLAS+FSGIVFCKIVYTLTGI+S QSFK YG+LSDFGKVEW NR
Sbjct: 1   MTISVTDVSVPSKELQWLASVFSGIVFCKIVYTLTGIVSLQSFKTYGKLSDFGKVEWKNR 60

Query: 159 GFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVL 218
           GFSTFHALVAASSSLYL+LLSSTF PGSS+ELLIRRTSSLSDMTLGFS+GYFLSDLAMVL
Sbjct: 61  GFSTFHALVAASSSLYLVLLSSTFDPGSSNELLIRRTSSLSDMTLGFSVGYFLSDLAMVL 120

Query: 219 WYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNK 278
           WYFPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG K
Sbjct: 121 WYFPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTECTTPFVNLRWYLDNAGQK 180

Query: 279 NSNFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMN 338
           NSN YV+NGIALF GWLVARILLFIYFF HM+++FD+VKTV+PLGFYSLL VPP+L+MMN
Sbjct: 181 NSNSYVINGIALFLGWLVARILLFIYFFGHMFKHFDQVKTVYPLGFYSLLTVPPMLAMMN 240

Query: 339 LFWFWKIARGMIKTIRKARHGK 361
           +FWFWKIARGMIKT+RKARH K
Sbjct: 241 VFWFWKIARGMIKTLRKARHNK 262

BLAST of Moc02g07490 vs. TAIR 10
Match: AT4G10360.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.3e-82
Identity = 145/248 (58.47%), Postives = 187/248 (75.40%), Query Frame = 0

Query: 110 NKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAA 169
           +++L  LASI SG + CKIVY LT  IS   F  YG+L    ++EWNNRGFSTFHA+  +
Sbjct: 12  SRQLLLLASICSGALMCKIVYDLTRFISPLLFSVYGKLDSKVRMEWNNRGFSTFHAVFTS 71

Query: 170 SSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEY 229
            +S+Y L++S  F      + +I  T+ LS+  +G S+GYFL+DLAM+ WYFP LGG+EY
Sbjct: 72  VASIYFLVISDQFDENVHGDSVINSTTRLSESVMGISLGYFLADLAMIFWYFPTLGGIEY 131

Query: 230 VLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIA 289
           V HH LS+F+I+ S+ SGQ Q YI +VL +E+TTPFVNLRWYLDN+G K S  Y +NGIA
Sbjct: 132 VFHHFLSMFAIILSVTSGQSQFYIFLVLLSEATTPFVNLRWYLDNSGQKGSKAYTLNGIA 191

Query: 290 LFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGM 349
           LF GWLVAR+LLFI+FF HMY +F +VK VFPLGFYSLL +PP L++MNL WFWKI +G+
Sbjct: 192 LFLGWLVARVLLFIFFFVHMYLHFHQVKQVFPLGFYSLLTIPPALAVMNLLWFWKITKGL 251

Query: 350 IKTIRKAR 358
           IKT+ KA+
Sbjct: 252 IKTLSKAK 259

BLAST of Moc02g07490 vs. TAIR 10
Match: AT4G10360.2 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.3e-82
Identity = 145/248 (58.47%), Postives = 187/248 (75.40%), Query Frame = 0

Query: 110 NKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAA 169
           +++L  LASI SG + CKIVY LT  IS   F  YG+L    ++EWNNRGFSTFHA+  +
Sbjct: 12  SRQLLLLASICSGALMCKIVYDLTRFISPLLFSVYGKLDSKVRMEWNNRGFSTFHAVFTS 71

Query: 170 SSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEY 229
            +S+Y L++S  F      + +I  T+ LS+  +G S+GYFL+DLAM+ WYFP LGG+EY
Sbjct: 72  VASIYFLVISDQFDENVHGDSVINSTTRLSESVMGISLGYFLADLAMIFWYFPTLGGIEY 131

Query: 230 VLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIA 289
           V HH LS+F+I+ S+ SGQ Q YI +VL +E+TTPFVNLRWYLDN+G K S  Y +NGIA
Sbjct: 132 VFHHFLSMFAIILSVTSGQSQFYIFLVLLSEATTPFVNLRWYLDNSGQKGSKAYTLNGIA 191

Query: 290 LFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGM 349
           LF GWLVAR+LLFI+FF HMY +F +VK VFPLGFYSLL +PP L++MNL WFWKI +G+
Sbjct: 192 LFLGWLVARVLLFIFFFVHMYLHFHQVKQVFPLGFYSLLTIPPALAVMNLLWFWKITKGL 251

Query: 350 IKTIRKAR 358
           IKT+ KA+
Sbjct: 252 IKTLSKAK 259

BLAST of Moc02g07490 vs. TAIR 10
Match: AT1G31300.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-61
Identity = 117/238 (49.16%), Postives = 160/238 (67.23%), Query Frame = 0

Query: 118 SIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAASSSLYLLL 177
           S+ +GI  CK+VY L   IS    K Y  L+   ++EWNNRG ST HA+  ++ SLY + 
Sbjct: 37  SVLTGIFLCKVVYDLCHFISNSHSKTYIILTKIQRIEWNNRGISTVHAIFISAMSLYFVF 96

Query: 178 LSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALSL 237
            S  F     ++L++ R+S LS + LG SIGYFL+DL M+ W +P+LGG+EY++HH+LS 
Sbjct: 97  WSDLFSDRWHNDLVVFRSSRLSSLGLGISIGYFLADLGMIFWKYPSLGGIEYIVHHSLSG 156

Query: 238 FSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIALFFGWLVA 297
            ++  SL SG+GQ+Y  MVL +E TTP +NLRWYLD AG K S  YVVNG+ +F  WLVA
Sbjct: 157 VAVAYSLFSGEGQLYTYMVLISEITTPEINLRWYLDTAGMKKSLAYVVNGVFIFLAWLVA 216

Query: 298 RILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGMIKTIRK 356
           RILLFIY F H+Y ++++V  +   G+  +  VP  L +MNL WF KI RG+ KT+ K
Sbjct: 217 RILLFIYMFYHVYLHYNQVMRMHIFGYVLVFGVPAALGVMNLIWFGKIVRGVKKTLAK 274

BLAST of Moc02g07490 vs. TAIR 10
Match: AT1G31300.2 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-61
Identity = 117/238 (49.16%), Postives = 160/238 (67.23%), Query Frame = 0

Query: 118 SIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAASSSLYLLL 177
           S+ +GI  CK+VY L   IS    K Y  L+   ++EWNNRG ST HA+  ++ SLY + 
Sbjct: 37  SVLTGIFLCKVVYDLCHFISNSHSKTYIILTKIQRIEWNNRGISTVHAIFISAMSLYFVF 96

Query: 178 LSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALSL 237
            S  F     ++L++ R+S LS + LG SIGYFL+DL M+ W +P+LGG+EY++HH+LS 
Sbjct: 97  WSDLFSDRWHNDLVVFRSSRLSSLGLGISIGYFLADLGMIFWKYPSLGGIEYIVHHSLSG 156

Query: 238 FSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIALFFGWLVA 297
            ++  SL SG+GQ+Y  MVL +E TTP +NLRWYLD AG K S  YVVNG+ +F  WLVA
Sbjct: 157 VAVAYSLFSGEGQLYTYMVLISEITTPEINLRWYLDTAGMKKSLAYVVNGVFIFLAWLVA 216

Query: 298 RILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGMIKTIRK 356
           RILLFIY F H+Y ++++V  +   G+  +  VP  L +MNL WF KI RG+ KT+ K
Sbjct: 217 RILLFIYMFYHVYLHYNQVMRMHIFGYVLVFGVPAALGVMNLIWFGKIVRGVKKTLAK 274

BLAST of Moc02g07490 vs. TAIR 10
Match: AT4G19645.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 229.2 bits (583), Expect = 5.3e-60
Identity = 117/239 (48.95%), Postives = 155/239 (64.85%), Query Frame = 0

Query: 118 SIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAASSSLYLLL 177
           S+  GI  CK+VY LT + S    K+Y  L+   ++EWNNRG ST HA+  +  +LY   
Sbjct: 28  SVLVGICLCKLVYDLTELFSSIHVKSYSALTKIKRIEWNNRGISTVHAIFISFMALYFAF 87

Query: 178 LSSTFHPGSS-DELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALS 237
            S  F    S + L + R S LS   LG S+GYFL+DL M+ W +P+LGG EY+LHH LS
Sbjct: 88  FSDLFSDQRSLETLTVFRNSPLSTFGLGVSVGYFLADLGMIFWLYPSLGGSEYILHHCLS 147

Query: 238 LFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIALFFGWLV 297
             ++  SL SG+ Q+Y  MVL +E TTP +NLRWYLD AG K S  Y+VNG+A+FF WL 
Sbjct: 148 GTAVAYSLFSGEAQLYTYMVLISEVTTPEINLRWYLDIAGLKRSKAYLVNGVAIFFAWLT 207

Query: 298 ARILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGMIKTIRK 356
           ARILLFIY F H+Y ++D+V  +   G+  +  VP  LS+MNL WF KI +G+ KT+ K
Sbjct: 208 ARILLFIYMFYHVYTHYDQVIEMHTFGYLLVFVVPISLSVMNLIWFGKIVKGLKKTLEK 266

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149739.15.8e-149100.00transmembrane protein 56-B isoform X1 [Momordica charantia] >XP_022149740.1 tran... [more]
XP_023533878.14.2e-13185.66transmembrane protein 56-B-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023007223.17.1e-13185.66transmembrane protein 56-B-like isoform X1 [Cucurbita maxima][more]
XP_022948078.11.2e-13085.29transmembrane protein 56-B-like isoform X1 [Cucurbita moschata][more]
KAG6570610.12.1e-13085.66TLC domain-containing protein 4-B, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q6PGS51.6e-1627.80TLC domain-containing protein 4-B OS=Xenopus laevis OX=8355 GN=tlcd4-b PE=2 SV=1[more]
Q96MV13.0e-1528.24TLC domain-containing protein 4 OS=Homo sapiens OX=9606 GN=TLCD4 PE=1 SV=1[more]
Q6P4N13.9e-1529.71TLC domain-containing protein 4 OS=Xenopus tropicalis OX=8364 GN=tlcd4 PE=2 SV=1[more]
Q6GLX21.1e-1428.26TLC domain-containing protein 4-A OS=Xenopus laevis OX=8355 GN=tlcd4-a PE=2 SV=1[more]
Q550S95.6e-1428.99TLC domain-containing protein 4 B OS=Dictyostelium discoideum OX=44689 GN=tlcd4b... [more]
Match NameE-valueIdentityDescription
A0A6J1D7X62.8e-149100.00transmembrane protein 56-B isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1L7443.4e-13185.66transmembrane protein 56-B-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1G8D25.8e-13185.29transmembrane protein 56-B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1JHF48.7e-12786.26transmembrane protein 56-B-like OS=Cucurbita maxima OX=3661 GN=LOC111484516 PE=4... [more]
A0A6J1FYI81.9e-12686.26transmembrane protein 56-B OS=Cucurbita moschata OX=3662 GN=LOC111448719 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT4G10360.11.3e-8258.47TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
AT4G10360.21.3e-8258.47TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
AT1G31300.11.3e-6149.16TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
AT1G31300.21.3e-6149.16TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
AT4G19645.15.3e-6048.95TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006634TRAM/LAG1/CLN8 homology domainSMARTSM00724lag1_27coord: 150..354
e-value: 6.6E-44
score: 161.9
IPR006634TRAM/LAG1/CLN8 homology domainPFAMPF03798TRAM_LAG1_CLN8coord: 154..347
e-value: 2.2E-30
score: 105.9
IPR006634TRAM/LAG1/CLN8 homology domainPROSITEPS50922TLCcoord: 150..354
score: 39.51865
NoneNo IPR availablePANTHERPTHR13439:SF54OS05G0485300 PROTEINcoord: 108..358
NoneNo IPR availablePANTHERPTHR13439CT120 PROTEINcoord: 108..358

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g07490.1Moc02g07490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055088 lipid homeostasis
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane