Moc02g02360 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g02360
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Germin-like protein
Location	chr2: 1802808 .. 1803416 (+)
RNA-Seq Expression	Moc02g02360
Synteny	Moc02g02360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCCCAAATTTGCAGCAGCGCTTCTCCTAACAACTGCCATGGCGGTGTCGGTGGCGGCGAGTGACCCATCTCTCCTCTCGGACTTCTTCTCCGCCAACGCCACCAAAATCGACGCCAATTACTTCACCTTCCACGGAATTCGCAAAGCCCTCTCCCAAAACCCTCCAAATTTCACGGCAACCAAAGCGAGCCTGGCCCAATTTCCGGCCAAGATTGGGCAGAGCGTTTCTCTGGCTCTTCTCCAGTTCCCGGCCGGCTCCGTCAACCCGCCGCACACCCACCCCCGCGCGGCGGAGCTTCTGCTCCTCGTTAAAGGCCGTCTTGAGGTCGGATTTGTTGATACCAATAACACGCTCTATAGTTCGAGGCTTGAGATGGGGGATTCGTTCTTGTTTCCCAAGGGATTGGTGCATTTTCAGTTCAATAGCGATGCGGATAATTCGGCTGTGGCTGTTTCCGGATTTGGAAGTGCGGACGCCGGCACGGTGGCTGTTCCGTCCGCCGTTTTTGGCTCCGGTATCGACGATGCGGCACTCGCCCTGTCTTTTAGAACGGACGTCGCCACCGTTCGCAAAATCAGAGCAGCTCTTGCTCCTCAAACCTAA

mRNA sequence

ATGGCGCCCAAATTTGCAGCAGCGCTTCTCCTAACAACTGCCATGGCGGTGTCGGTGGCGGCGAGTGACCCATCTCTCCTCTCGGACTTCTTCTCCGCCAACGCCACCAAAATCGACGCCAATTACTTCACCTTCCACGGAATTCGCAAAGCCCTCTCCCAAAACCCTCCAAATTTCACGGCAACCAAAGCGAGCCTGGCCCAATTTCCGGCCAAGATTGGGCAGAGCGTTTCTCTGGCTCTTCTCCAGTTCCCGGCCGGCTCCGTCAACCCGCCGCACACCCACCCCCGCGCGGCGGAGCTTCTGCTCCTCGTTAAAGGCCGTCTTGAGGTCGGATTTGTTGATACCAATAACACGCTCTATAGTTCGAGGCTTGAGATGGGGGATTCGTTCTTGTTTCCCAAGGGATTGGTGCATTTTCAGTTCAATAGCGATGCGGATAATTCGGCTGTGGCTGTTTCCGGATTTGGAAGTGCGGACGCCGGCACGGTGGCTGTTCCGTCCGCCGTTTTTGGCTCCGGTATCGACGATGCGGCACTCGCCCTGTCTTTTAGAACGGACGTCGCCACCGTTCGCAAAATCAGAGCAGCTCTTGCTCCTCAAACCTAA

Coding sequence (CDS)

ATGGCGCCCAAATTTGCAGCAGCGCTTCTCCTAACAACTGCCATGGCGGTGTCGGTGGCGGCGAGTGACCCATCTCTCCTCTCGGACTTCTTCTCCGCCAACGCCACCAAAATCGACGCCAATTACTTCACCTTCCACGGAATTCGCAAAGCCCTCTCCCAAAACCCTCCAAATTTCACGGCAACCAAAGCGAGCCTGGCCCAATTTCCGGCCAAGATTGGGCAGAGCGTTTCTCTGGCTCTTCTCCAGTTCCCGGCCGGCTCCGTCAACCCGCCGCACACCCACCCCCGCGCGGCGGAGCTTCTGCTCCTCGTTAAAGGCCGTCTTGAGGTCGGATTTGTTGATACCAATAACACGCTCTATAGTTCGAGGCTTGAGATGGGGGATTCGTTCTTGTTTCCCAAGGGATTGGTGCATTTTCAGTTCAATAGCGATGCGGATAATTCGGCTGTGGCTGTTTCCGGATTTGGAAGTGCGGACGCCGGCACGGTGGCTGTTCCGTCCGCCGTTTTTGGCTCCGGTATCGACGATGCGGCACTCGCCCTGTCTTTTAGAACGGACGTCGCCACCGTTCGCAAAATCAGAGCAGCTCTTGCTCCTCAAACCTAA

Protein sequence

MAPKFAAALLLTTAMAVSVAASDPSLLSDFFSANATKIDANYFTFHGIRKALSQNPPNFTATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAALALSFRTDVATVRKIRAALAPQT

Homology

[Close]

BLAST of Moc02g02360 vs. NCBI nr
Match: XP_022152984.1 (germin-like protein 9-3 [Momordica charantia])

HSP 1 Score: 380.6 bits (976), Expect = 8.6e-102
Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 1   MAPKFAAALLLTTAMAVSVAASDPSLLSDFFSANATKIDANYFTFHGIRKALSQNPPNFT 60
           MAPKFAAALLLTTAMAVSVAASDPSLLSDFFSANATKIDANYFTFHGIRKALSQNPPNFT
Sbjct: 1   MAPKFAAALLLTTAMAVSVAASDPSLLSDFFSANATKIDANYFTFHGIRKALSQNPPNFT 60

Query: 61  ATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTL 120
           ATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTL
Sbjct: 61  ATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTL 120

Query: 121 YSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL 180
           YSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL
Sbjct: 121 YSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL 180

Query: 181 ALSFRTDVATVRKIRAALAPQT 203
           ALSFRTDVATVRKIRAALAPQT
Sbjct: 181 ALSFRTDVATVRKIRAALAPQT 202

[Close]

BLAST of Moc02g02360 vs. NCBI nr
Match: XP_040991201.1 (germin-like protein 9-3 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 233.8 bits (595), Expect = 1.3e-57
Identity = 119/201 (59.20%), Postives = 157/201 (78.11%), Query Frame = 0


Query: 4   KFAAALLLTTAMAVSVA-ASDPSLLSDFFS-ANATKIDANYFTFHGIRKALSQNPPNFTA 63
           K++   LL       VA ASDP++LSDF + AN+T +D NYFTF+G+R   SQ PPNF  
Sbjct: 7   KYSVFTLLAVFATFWVAKASDPNILSDFIAPANSTSVDGNYFTFNGLRGIFSQTPPNFKV 66

Query: 64  TKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTLY 123
           TKAS+ +FPA  GQSVS+A+LQFP+G VNPPHTHPR+AELL LV+G LEVGF+DT N LY
Sbjct: 67  TKASMVEFPALNGQSVSIAVLQFPSGGVNPPHTHPRSAELLFLVQGSLEVGFIDTTNKLY 126

Query: 124 SSRLEMGDSFLFPKGLVHFQFNSD-ADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL 183
           +  L++GD F+FPKGLVHFQ+N+D A+ +A A+S FGSA+AGT+++P+ VF +GIDDA L
Sbjct: 127 NQTLQVGDMFIFPKGLVHFQYNADQANRTATAISAFGSANAGTISIPANVFTTGIDDAIL 186

Query: 184 ALSFRTDVATVRKIRAALAPQ 202
           A +F+TD AT++ I+  LAP+
Sbjct: 187 AKAFKTDTATIQNIKVGLAPK 207

[Close]

BLAST of Moc02g02360 vs. NCBI nr
Match: XP_018831257.1 (germin-like protein 9-3 [Juglans regia] >KAF5447484.1 hypothetical protein F2P56_033038 [Juglans regia])

HSP 1 Score: 233.4 bits (594), Expect = 1.7e-57
Identity = 120/201 (59.70%), Postives = 155/201 (77.11%), Query Frame = 0


Query: 4   KFAAALLLTTAMAVSVA-ASDPSLLSDFFS-ANATKIDANYFTFHGIRKALSQNPPNFTA 63
           K++   LL       VA ASDP++LSDF + AN+T +D NYFTF+G+R   SQ PPNF  
Sbjct: 7   KYSVFTLLAVFATFWVAKASDPNILSDFIAPANSTSVDGNYFTFNGLRGIFSQTPPNFKV 66

Query: 64  TKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTLY 123
           TKAS+ +FPA  GQSVS+A+LQFP G VNPPHTHPR+AELL LV+G LEVGF+DT N LY
Sbjct: 67  TKASMVEFPALNGQSVSIAVLQFPTGGVNPPHTHPRSAELLFLVQGSLEVGFIDTTNKLY 126

Query: 124 SSRLEMGDSFLFPKGLVHFQFNSDADN-SAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL 183
           +  L++GD F+FPKGLVHFQ+N+DA N +A A+S FGSA+AGT ++P+ VF +GIDDA L
Sbjct: 127 NQTLQVGDMFIFPKGLVHFQYNADAANRTATAISAFGSANAGTSSIPATVFTTGIDDAIL 186

Query: 184 ALSFRTDVATVRKIRAALAPQ 202
           A +F+TD AT++ I+  LAP+
Sbjct: 187 AKAFKTDTATIQNIKVGLAPK 207

[Close]

BLAST of Moc02g02360 vs. NCBI nr
Match: KAF6165878.1 (hypothetical protein GIB67_012775 [Kingdonia uniflora])

HSP 1 Score: 227.3 bits (578), Expect = 1.2e-55
Identity = 119/193 (61.66%), Postives = 149/193 (77.20%), Query Frame = 0


Query: 9   LLLTTAMAVSVAASDPSLLSDF-FSANA-TKIDANYFTFHGIRKALSQNPPNFTATKASL 68
           LL+   M+  V ASDP + SDF   ANA T ID  +FT+ G+R  +   PP F  TKASL
Sbjct: 13  LLVAHTMSQMVYASDPDITSDFIIPANANTFIDGAFFTYTGLRGVVGATPPAFKVTKASL 72

Query: 69  AQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTLYSSRLE 128
           A+FPA  GQSVSLA+LQFPAG VNPPHTHPR+AELL +V+G LEVGFVDT N LY+  L+
Sbjct: 73  AEFPALDGQSVSLAVLQFPAGGVNPPHTHPRSAELLFVVEGSLEVGFVDTKNVLYTQTLQ 132

Query: 129 MGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAALALSFRT 188
           +GD F+FPKGLVHFQ+N++A   A A+SGFGSA+AGTV++P+ +F +GIDD  LA SF+T
Sbjct: 133 LGDMFIFPKGLVHFQYNANAKVPATAISGFGSANAGTVSIPATIFATGIDDGILAKSFKT 192

Query: 189 DVATVRKIRAALA 200
           DVAT++KI+A LA
Sbjct: 193 DVATIQKIKAGLA 205

[Close]

BLAST of Moc02g02360 vs. NCBI nr
Match: RWR82312.1 (putative germin-like protein 9-2 [Cinnamomum micranthum f. kanehirae])

HSP 1 Score: 227.3 bits (578), Expect = 1.2e-55
Identity = 118/192 (61.46%), Postives = 148/192 (77.08%), Query Frame = 0


Query: 11  LTTAMAVSVAASDPSLLSDF-FSANATKIDANYFTFHGIRKALSQNPPNFTATKASLAQF 70
           L T +A    A DP+++SDF   AN + +D N+FT+ G+R   +  P  F  TK S+A+F
Sbjct: 38  LITTIAQIANAGDPNIVSDFILPANLSIVDGNFFTYTGLRALANGFPDTFKVTKVSMAEF 97

Query: 71  PAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTLYSSRLEMGD 130
           PA  GQSVS A+LQ+PAG+VNPPHTHPR+AELL L+ G LEVGFVDT+N LY+  L  GD
Sbjct: 98  PALNGQSVSYAVLQYPAGTVNPPHTHPRSAELLFLIDGALEVGFVDTSNKLYTQILHSGD 157

Query: 131 SFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAALALSFRTDVA 190
            F+FPKGLVH+Q+NSD +NSAVA+S FGSA AGTV+VPS+VF +GID+A LA SFRTDVA
Sbjct: 158 IFVFPKGLVHYQYNSDVNNSAVALSAFGSASAGTVSVPSSVFTTGIDEAILAKSFRTDVA 217

Query: 191 TVRKIRAALAPQ 202
           TV KI+A LAP+
Sbjct: 218 TVEKIKAGLAPK 229

[Close]

BLAST of Moc02g02360 vs. ExPASy Swiss-Prot
Match: Q652P9 (Germin-like protein 9-3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568700 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.3e-49
Identity = 112/210 (53.33%), Postives = 143/210 (68.10%), Query Frame = 0


Query: 6   AAALLLTTAMAV------SVAASDPSLLSDFF---SANATKIDANYFTFHGIRKALSQN- 65
           A+++LL   +AV       V A DP +L+D+     +NA  I  ++FTF G R  LS N 
Sbjct: 2   ASSILLLVVLAVVSAPVALVMAGDPDILTDYVIPAGSNAENITGDFFTFTGFRNPLSMNM 61

Query: 66  -----PPNFTATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLE 125
                  NFT TKA++A+FPA  GQSVS A+L +P  ++NPPHTHPR+AELLLLV G L 
Sbjct: 62  SMPMPNANFTVTKATMAEFPALNGQSVSYAVLMYPPATLNPPHTHPRSAELLLLVDGALS 121

Query: 126 VGFVDTNNTLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAV 185
           VGFVDT N LY+  L  GD F+FPKG+VHFQFNS  +  A+A+S FGSA AG V+VP  V
Sbjct: 122 VGFVDTTNKLYTQDLAAGDMFVFPKGMVHFQFNS-GNQPAMALSAFGSAAAGLVSVPVTV 181

Query: 186 FGSGIDDAALALSFRTDVATVRKIRAALAP 201
           FG+ IDDA LA SF+TDV T++K++A L P
Sbjct: 182 FGTNIDDAVLAKSFKTDVPTIQKLKAGLTP 210

[Close]

BLAST of Moc02g02360 vs. ExPASy Swiss-Prot
Match: Q652Q0 (Putative germin-like protein 9-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568600 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.2e-47
Identity = 110/211 (52.13%), Postives = 140/211 (66.35%), Query Frame = 0


Query: 1   MAPKFAAALLLTTAM----AVSVAASDPSLLSDFF---SANATKIDANYFTFHGIRKALS 60
           MA  + + LLL  A+       V A DP +L+D+    + N   I  ++FTF G RK  +
Sbjct: 1   MALSYYSLLLLLLAVWAPALTLVMAGDPDILTDYVIPANGNPMNITGDFFTFTGFRKVFN 60

Query: 61  QN----PPNFTATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRL 120
            +    P +FT TKA++A+FPA  GQSVS A L FP  +VNPPHTHPR+AELLL+V G L
Sbjct: 61  TSSAPEPNSFTVTKATMAEFPALNGQSVSYATLVFPPSTVNPPHTHPRSAELLLVVDGAL 120

Query: 121 EVGFVDTNNTLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSA 180
            VGF+DT N LY+  L  GD F+FPKG+VHFQFNS  +  A+A+S FGSA  G V VP  
Sbjct: 121 SVGFIDTTNKLYTQDLAAGDMFVFPKGMVHFQFNS-GNQPAMALSAFGSAAPGVVPVPVT 180

Query: 181 VFGSGIDDAALALSFRTDVATVRKIRAALAP 201
           VFG+GIDDA LA SF+TDV T+ K++A L P
Sbjct: 181 VFGTGIDDAVLAKSFKTDVPTILKLKANLTP 210

[Close]

BLAST of Moc02g02360 vs. ExPASy Swiss-Prot
Match: Q652Q1 (Germin-like protein 9-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568500 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.2e-43
Identity = 99/197 (50.25%), Postives = 132/197 (67.01%), Query Frame = 0


Query: 9   LLLTTAMAVSVAASDPSLLSDFFSANATK---IDANYFTFHGIRKALSQNPPNFTATKAS 68
           +LL  A+ +S  A+DP +L+DF   + T    ID  +FT+  +    S +P   T TKA+
Sbjct: 13  VLLLLAVILSAGAADPDILTDFVVPSDTDPSGIDGAFFTYKNLVTGNSGDPAKLTVTKAT 72

Query: 69  LAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDT--NNTLYSS 128
            A+FPA +GQSVS A L F AG+VNPPH HPRA+ELL++V+G L VG VD   N T+Y+ 
Sbjct: 73  HAEFPALLGQSVSYAALVFGAGTVNPPHIHPRASELLVVVQGPLLVGLVDAARNGTVYTQ 132

Query: 129 RLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAALALS 188
            L+ GD F+FPKG+VHFQFN+  D  A A S FGSA  GT+++P+A+FGSGIDD  L  S
Sbjct: 133 TLQTGDMFVFPKGMVHFQFNNGTDVVARAFSAFGSATPGTISLPAALFGSGIDDTILDKS 192

Query: 189 FRTDVATVRKIRAALAP 201
             TD ATV +++   AP
Sbjct: 193 MHTDQATVDQLKQDQAP 209

[Close]

BLAST of Moc02g02360 vs. ExPASy Swiss-Prot
Match: Q9M8X6 (Germin-like protein subfamily 1 member 6 OS=Arabidopsis thaliana OX=3702 GN=At3g04200 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.4e-24
Identity = 82/220 (37.27%), Postives = 114/220 (51.82%), Query Frame = 0


Query: 9   LLLTTAMAVSVAAS-----DPSLLSDF---------------FSANATKIDANYFTFHGI 68
           LL+T  + +++A S     DP+ L DF               F  +   + AN F++ G+
Sbjct: 8   LLVTQVILLALATSFVSCYDPNPLQDFCVAASETNRVFVNGKFCKDPKSVTANDFSYSGL 67

Query: 69  RKALSQNPPNF---TATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLV 128
              +++N  NF     T   + + P      VSLA L F  G  NPPH HPRA E+L++ 
Sbjct: 68  N--IARNTTNFLGSNVTTVDVNKIPGLNTLGVSLARLDFAQGGQNPPHIHPRATEILVVT 127

Query: 129 KGRLEVGFVDT---NNTLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAG 188
           KG+L VGFV +   NN L+   L+ GD F+FP GL+HFQ N      AVA +GFGS + G
Sbjct: 128 KGKLLVGFVSSNQDNNRLFYKVLKRGDVFVFPIGLIHFQMNV-RRTRAVAFAGFGSQNPG 187

Query: 189 TVAVPSAVFGS--GIDDAALALSFRTDVATVRKIRAALAP 201
           T+ +  AVFGS   I    LA +F+ DV  VR +     P
Sbjct: 188 TIRIADAVFGSNPSIPQEVLAKAFQLDVKLVRFLHIVFGP 224

[Close]

BLAST of Moc02g02360 vs. ExPASy Swiss-Prot
Match: Q6YZA1 (Germin-like protein 8-8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0189700 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 5.7e-24
Identity = 76/214 (35.51%), Postives = 110/214 (51.40%), Query Frame = 0


Query: 2   APKFAAALLLTTAMAVSVAASDPSLLSDFFSANATK---------IDANYFTFHGIRKAL 61
           +P F     L   ++    ASDPS L DF  A+            +D  Y T     KA 
Sbjct: 3   SPSFCLLAALLALVSWQAIASDPSPLQDFCVADKHSPVLVNGFACLDPKYVTADHFFKAA 62

Query: 62  SQNPPNFT------ATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVK 121
             + P  T       T  ++ Q P      +S+A + +     NPPHTHPRA E+L +++
Sbjct: 63  MLDTPRKTNKVGSNVTLINVMQIPGLNTLGISIARIDYAPLGENPPHTHPRATEILTVLE 122

Query: 122 GRLEVGFV--DTNNTLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTV 181
           G L VGFV  + NNTL+S  L  GD F+FP+GL+HFQFN +    AVA++   S + G +
Sbjct: 123 GTLYVGFVTSNPNNTLFSKVLNKGDVFVFPEGLIHFQFNPNPHQPAVAIAALSSQNPGAI 182

Query: 182 AVPSAVFGS--GIDDAALALSFRTDVATVRKIRA 197
            + +AVFGS   I D  LA +F+ +  T+  ++A
Sbjct: 183 TIANAVFGSKPPISDKVLAKAFQVEKGTIDWLQA 216

[Close]

BLAST of Moc02g02360 vs. ExPASy TrEMBL
Match: A0A6J1DHP2 (Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111020591 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.2e-102
Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 1   MAPKFAAALLLTTAMAVSVAASDPSLLSDFFSANATKIDANYFTFHGIRKALSQNPPNFT 60
           MAPKFAAALLLTTAMAVSVAASDPSLLSDFFSANATKIDANYFTFHGIRKALSQNPPNFT
Sbjct: 1   MAPKFAAALLLTTAMAVSVAASDPSLLSDFFSANATKIDANYFTFHGIRKALSQNPPNFT 60

Query: 61  ATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTL 120
           ATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTL
Sbjct: 61  ATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTL 120

Query: 121 YSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL 180
           YSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL
Sbjct: 121 YSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL 180

Query: 181 ALSFRTDVATVRKIRAALAPQT 203
           ALSFRTDVATVRKIRAALAPQT
Sbjct: 181 ALSFRTDVATVRKIRAALAPQT 202

[Close]

BLAST of Moc02g02360 vs. ExPASy TrEMBL
Match: A0A2I4FHZ0 (Germin-like protein OS=Juglans regia OX=51240 GN=LOC108998942 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 8.3e-58
Identity = 120/201 (59.70%), Postives = 155/201 (77.11%), Query Frame = 0


Query: 4   KFAAALLLTTAMAVSVA-ASDPSLLSDFFS-ANATKIDANYFTFHGIRKALSQNPPNFTA 63
           K++   LL       VA ASDP++LSDF + AN+T +D NYFTF+G+R   SQ PPNF  
Sbjct: 7   KYSVFTLLAVFATFWVAKASDPNILSDFIAPANSTSVDGNYFTFNGLRGIFSQTPPNFKV 66

Query: 64  TKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTLY 123
           TKAS+ +FPA  GQSVS+A+LQFP G VNPPHTHPR+AELL LV+G LEVGF+DT N LY
Sbjct: 67  TKASMVEFPALNGQSVSIAVLQFPTGGVNPPHTHPRSAELLFLVQGSLEVGFIDTTNKLY 126

Query: 124 SSRLEMGDSFLFPKGLVHFQFNSDADN-SAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL 183
           +  L++GD F+FPKGLVHFQ+N+DA N +A A+S FGSA+AGT ++P+ VF +GIDDA L
Sbjct: 127 NQTLQVGDMFIFPKGLVHFQYNADAANRTATAISAFGSANAGTSSIPATVFTTGIDDAIL 186

Query: 184 ALSFRTDVATVRKIRAALAPQ 202
           A +F+TD AT++ I+  LAP+
Sbjct: 187 AKAFKTDTATIQNIKVGLAPK 207

[Close]

BLAST of Moc02g02360 vs. ExPASy TrEMBL
Match: A0A3S3QAC0 (Germin-like protein OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_01102800 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 5.9e-56
Identity = 118/192 (61.46%), Postives = 148/192 (77.08%), Query Frame = 0


Query: 11  LTTAMAVSVAASDPSLLSDF-FSANATKIDANYFTFHGIRKALSQNPPNFTATKASLAQF 70
           L T +A    A DP+++SDF   AN + +D N+FT+ G+R   +  P  F  TK S+A+F
Sbjct: 38  LITTIAQIANAGDPNIVSDFILPANLSIVDGNFFTYTGLRALANGFPDTFKVTKVSMAEF 97

Query: 71  PAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTLYSSRLEMGD 130
           PA  GQSVS A+LQ+PAG+VNPPHTHPR+AELL L+ G LEVGFVDT+N LY+  L  GD
Sbjct: 98  PALNGQSVSYAVLQYPAGTVNPPHTHPRSAELLFLIDGALEVGFVDTSNKLYTQILHSGD 157

Query: 131 SFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAALALSFRTDVA 190
            F+FPKGLVH+Q+NSD +NSAVA+S FGSA AGTV+VPS+VF +GID+A LA SFRTDVA
Sbjct: 158 IFVFPKGLVHYQYNSDVNNSAVALSAFGSASAGTVSVPSSVFTTGIDEAILAKSFRTDVA 217

Query: 191 TVRKIRAALAPQ 202
           TV KI+A LAP+
Sbjct: 218 TVEKIKAGLAPK 229

[Close]

BLAST of Moc02g02360 vs. ExPASy TrEMBL
Match: A0A7J7NG83 (Germin-like protein OS=Kingdonia uniflora OX=39325 GN=GIB67_012775 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 5.9e-56
Identity = 119/193 (61.66%), Postives = 149/193 (77.20%), Query Frame = 0


Query: 9   LLLTTAMAVSVAASDPSLLSDF-FSANA-TKIDANYFTFHGIRKALSQNPPNFTATKASL 68
           LL+   M+  V ASDP + SDF   ANA T ID  +FT+ G+R  +   PP F  TKASL
Sbjct: 13  LLVAHTMSQMVYASDPDITSDFIIPANANTFIDGAFFTYTGLRGVVGATPPAFKVTKASL 72

Query: 69  AQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTLYSSRLE 128
           A+FPA  GQSVSLA+LQFPAG VNPPHTHPR+AELL +V+G LEVGFVDT N LY+  L+
Sbjct: 73  AEFPALDGQSVSLAVLQFPAGGVNPPHTHPRSAELLFVVEGSLEVGFVDTKNVLYTQTLQ 132

Query: 129 MGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAALALSFRT 188
           +GD F+FPKGLVHFQ+N++A   A A+SGFGSA+AGTV++P+ +F +GIDD  LA SF+T
Sbjct: 133 LGDMFIFPKGLVHFQYNANAKVPATAISGFGSANAGTVSIPATIFATGIDDGILAKSFKT 192

Query: 189 DVATVRKIRAALA 200
           DVAT++KI+A LA
Sbjct: 193 DVATIQKIKAGLA 205

[Close]

BLAST of Moc02g02360 vs. ExPASy TrEMBL
Match: A0A175YEM0 (Germin-like protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_029274 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 7.7e-56
Identity = 118/201 (58.71%), Postives = 149/201 (74.13%), Query Frame = 0


Query: 9   LLLTTAMAVSVA-ASDPSLLSDFF------SANATKIDANYFTFHGIRKAL-SQNPPNFT 68
           LL+ T   + +A A DP +L+DF       S N T ID N+FTF G+R  +  + PP F 
Sbjct: 13  LLVATFATIEIAVAGDPDILTDFIVPANLTSGNGTSIDGNFFTFTGMRVLVGGETPPAFK 72

Query: 69  ATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNNTL 128
             KAS+A+FPA  GQSV  A LQFPAGS NPPHTHPRA+ELL +V G LEVGF+DT N L
Sbjct: 73  VLKASMAEFPALNGQSVMYAALQFPAGSTNPPHTHPRASELLFVVDGSLEVGFIDTTNKL 132

Query: 129 YSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGSGIDDAAL 188
           Y+  L+ GD F+FPKGLVH+QFN+DA  SA+A+SGFGS++AGTV++PS VF +GIDD  L
Sbjct: 133 YTQTLQTGDMFVFPKGLVHYQFNADAQKSALAISGFGSSNAGTVSIPSTVFATGIDDNIL 192

Query: 189 ALSFRTDVATVRKIRAALAPQ 202
           ALSF+TDVAT++KI+A L P+
Sbjct: 193 ALSFKTDVATIKKIKAGLVPK 213

[Close]

BLAST of Moc02g02360 vs. TAIR 10
Match: AT3G04200.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 2.4e-25
Identity = 82/220 (37.27%), Postives = 114/220 (51.82%), Query Frame = 0


Query: 9   LLLTTAMAVSVAAS-----DPSLLSDF---------------FSANATKIDANYFTFHGI 68
           LL+T  + +++A S     DP+ L DF               F  +   + AN F++ G+
Sbjct: 8   LLVTQVILLALATSFVSCYDPNPLQDFCVAASETNRVFVNGKFCKDPKSVTANDFSYSGL 67

Query: 69  RKALSQNPPNF---TATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLV 128
              +++N  NF     T   + + P      VSLA L F  G  NPPH HPRA E+L++ 
Sbjct: 68  N--IARNTTNFLGSNVTTVDVNKIPGLNTLGVSLARLDFAQGGQNPPHIHPRATEILVVT 127

Query: 129 KGRLEVGFVDT---NNTLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAG 188
           KG+L VGFV +   NN L+   L+ GD F+FP GL+HFQ N      AVA +GFGS + G
Sbjct: 128 KGKLLVGFVSSNQDNNRLFYKVLKRGDVFVFPIGLIHFQMNV-RRTRAVAFAGFGSQNPG 187

Query: 189 TVAVPSAVFGS--GIDDAALALSFRTDVATVRKIRAALAP 201
           T+ +  AVFGS   I    LA +F+ DV  VR +     P
Sbjct: 188 TIRIADAVFGSNPSIPQEVLAKAFQLDVKLVRFLHIVFGP 224

[Close]

BLAST of Moc02g02360 vs. TAIR 10
Match: AT1G09560.1 (germin-like protein 5 )

HSP 1 Score: 111.3 bits (277), Expect = 9.1e-25
Identity = 75/217 (34.56%), Postives = 114/217 (52.53%), Query Frame = 0


Query: 2   APKFAAALLLTTAMAVSVAASDPSLLSDFFSAN---------------ATKIDANYFTFH 61
           +P     LLLTT      +++DP +L D   A+               AT   A++F+  
Sbjct: 3   SPTLTLLLLLTTVSFFISSSADPDMLQDLCVADLPSGIKINGFPCKDAATVTSADFFSQG 62

Query: 62  GIRKALSQNPPNFTATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVK 121
             +  L+ N      T A++   P      VSL+ + +  G +NPPHTHPRA E++ +++
Sbjct: 63  LAKPGLTNNTFGALVTGANVMTIPGLNTLGVSLSRIDYAPGGLNPPHTHPRATEVVFVLE 122

Query: 122 GRLEVGFVDTNNTLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAV 181
           G L+VGF+ T N L S  L+ GD F FPKGLVHFQ N + D  A  ++ F S   GT ++
Sbjct: 123 GTLDVGFLTTANKLISQSLKKGDVFAFPKGLVHFQKN-NGDVPASVIAAFNSQLPGTQSL 182

Query: 182 PSAVFGS--GIDDAALALSFRTDVATVRKIRAALAPQ 202
            + +FGS   + D  LA +F+T   TV+ I++   P+
Sbjct: 183 GATLFGSTPPVPDNILAQAFQTSPGTVKHIKSKFQPK 218

[Close]

BLAST of Moc02g02360 vs. TAIR 10
Match: AT1G18980.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 108.6 bits (270), Expect = 5.9e-24
Identity = 73/210 (34.76%), Postives = 113/210 (53.81%), Query Frame = 0


Query: 5   FAAALLLTTAMAVSVAASDPSLLSDFFSAN------------ATKIDANYFTFHGIRKAL 64
           F + LL+     +   +SD   L DF   +             + + A+ F F G+   L
Sbjct: 11  FRSFLLVICVFVIPSLSSDSDPLQDFCVGDLKASPSINGFPCKSSVSASDFFFSGLGGPL 70

Query: 65  SQNPPNFTA-TKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEV 124
           + + PN  A + A++  FP      +S+  ++F  G VNPPH+HPRA E  ++++G + V
Sbjct: 71  NTSTPNGVAVSPANVLTFPGLNTLGLSMNNVEFAPGGVNPPHSHPRATEAGVVIEGSVFV 130

Query: 125 GFVDTNNTLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVF 184
           GF+ TNNTL+S  L  G+ F+ P+GLVHFQ+N      A  ++ F S   G+  +PS +F
Sbjct: 131 GFLTTNNTLFSKVLNAGEMFVVPRGLVHFQWNV-GKVKARLITSFNSQLPGSAVLPSTLF 190

Query: 185 GSG--IDDAALALSFRTDVATVRKIRAALA 200
           GS   I +A L  +FRTD  TV K+++  A
Sbjct: 191 GSNPTIPNAVLTKTFRTDDVTVNKLKSKFA 219

[Close]

BLAST of Moc02g02360 vs. TAIR 10
Match: AT3G62020.1 (germin-like protein 10 )

HSP 1 Score: 107.8 bits (268), Expect = 1.0e-23
Identity = 68/205 (33.17%), Postives = 108/205 (52.68%), Query Frame = 0


Query: 14  AMAVSVAASDPSLLSDFFSANAT--------------KIDANYFTFHGIRK-ALSQNPPN 73
           ++ V V A DP  L D   A+ T               I A+ F F GI K A+  N   
Sbjct: 14  SLNVIVLAYDPDTLQDLCVADRTSGIKVNGFTCKPESNITASDFFFAGIGKPAVVNNTVG 73

Query: 74  FTATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGRLEVGFVDTNN 133
              T A++ +        VSLA + +  G +NPPHTHPRA E++ +++G L+VGF+ T N
Sbjct: 74  SAVTGANVEKIAGLNTLGVSLARIDYAPGGLNPPHTHPRATEVIFVLEGELDVGFITTAN 133

Query: 134 TLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPSAVFGS--GID 193
            L++  ++ G+ F+FP+GL+H+Q N+D    A  +S F S   GT ++ + +F +   I 
Sbjct: 134 KLFAKTVKKGEVFVFPRGLIHYQKNNDKAKPASVISAFNSQLPGTQSIAATLFTATPAIP 193

Query: 194 DAALALSFRTDVATVRKIRAALAPQ 202
           D  L  +F+     + KI++  AP+
Sbjct: 194 DHVLTTTFQIGTKEIEKIKSKFAPK 218

[Close]

BLAST of Moc02g02360 vs. TAIR 10
Match: AT3G10080.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.3e-23
Identity = 71/212 (33.49%), Postives = 100/212 (47.17%), Query Frame = 0


Query: 8   ALLLTTAMAVSVAASDPSLLSDF--------------FSA-----NATKIDANYFTFHGI 67
           AL L         ASDP  + DF              FS      N++++    F F G+
Sbjct: 11  ALCLLCFNVCFTLASDPDPIQDFCIPKPVTSPYHDHHFSTNLPCKNSSEVTTEDFVFSGL 70

Query: 68  RKALSQNPPNFTATKASLAQFPAKIGQSVSLALLQFPAGSVNPPHTHPRAAELLLLVKGR 127
           + A +     F         FP      +S        GS+NPPH HPRA E+  LVKGR
Sbjct: 71  KTAGNFTETGFATVPVGPENFPGLNTLGISFVRADLKPGSINPPHYHPRATEVAHLVKGR 130

Query: 128 LEVGFVDTNNTLYSSRLEMGDSFLFPKGLVHFQFNSDADNSAVAVSGFGSADAGTVAVPS 187
           +  GFVD+NN +Y+  +E G+  ++PKGLVHFQ N   D +A  V G  S + G   +PS
Sbjct: 131 VYSGFVDSNNKVYAKVMEEGEMMVYPKGLVHFQMNV-GDVTATIVGGLNSQNPGIQKIPS 190

Query: 188 AVFGSGIDDAALALSFRTDVATVRKIRAALAP 201
            VFGSGI++  L  +F   +  +  ++    P
Sbjct: 191 VVFGSGINEELLMKAFGLSLKQIGTLKKRFDP 221

The following BLAST results are available for this feature:

BLAST of Moc02g02360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152984.1	8.6e-102	100.00	germin-like protein 9-3 [Momordica charantia]	[more]
XP_040991201.1	1.3e-57	59.20	germin-like protein 9-3 [Juglans microcarpa x Juglans regia]	[more]
XP_018831257.1	1.7e-57	59.70	germin-like protein 9-3 [Juglans regia] >KAF5447484.1 hypothetical protein F2P56...	[more]
KAF6165878.1	1.2e-55	61.66	hypothetical protein GIB67_012775 [Kingdonia uniflora]	[more]
RWR82312.1	1.2e-55	61.46	putative germin-like protein 9-2 [Cinnamomum micranthum f. kanehirae]	[more]

BLAST of Moc02g02360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q652P9	2.3e-49	53.33	Germin-like protein 9-3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568700...	[more]
Q652Q0	2.2e-47	52.13	Putative germin-like protein 9-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q652Q1	4.2e-43	50.25	Germin-like protein 9-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568500...	[more]
Q9M8X6	3.4e-24	37.27	Germin-like protein subfamily 1 member 6 OS=Arabidopsis thaliana OX=3702 GN=At3g...	[more]
Q6YZA1	5.7e-24	35.51	Germin-like protein 8-8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0189700...	[more]

BLAST of Moc02g02360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHP2	4.2e-102	100.00	Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111020591 PE=3 SV=1	[more]
A0A2I4FHZ0	8.3e-58	59.70	Germin-like protein OS=Juglans regia OX=51240 GN=LOC108998942 PE=3 SV=1	[more]
A0A3S3QAC0	5.9e-56	61.46	Germin-like protein OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_0110...	[more]
A0A7J7NG83	5.9e-56	61.66	Germin-like protein OS=Kingdonia uniflora OX=39325 GN=GIB67_012775 PE=3 SV=1	[more]
A0A175YEM0	7.7e-56	58.71	Germin-like protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_029274 PE=3...	[more]

BLAST of Moc02g02360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G04200.1	2.4e-25	37.27	RmlC-like cupins superfamily protein	[more]
AT1G09560.1	9.1e-25	34.56	germin-like protein 5	[more]
AT1G18980.1	5.9e-24	34.76	RmlC-like cupins superfamily protein	[more]
AT3G62020.1	1.0e-23	33.17	germin-like protein 10	[more]
AT3G10080.1	1.3e-23	33.49	RmlC-like cupins superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001929	Germin	PRINTS	PR00325	GERMIN	coord: 123..143 score: 52.82 coord: 93..113 score: 54.6 coord: 157..172 score: 34.45
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 46..192 e-value: 6.4E-36 score: 135.3
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 55..189 e-value: 6.4E-26 score: 90.7
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 22..200 e-value: 9.7E-52 score: 177.3
None	No IPR available	PANTHER	PTHR31238:SF154	GERMIN-LIKE PROTEIN	coord: 9..196
None	No IPR available	PANTHER	PTHR31238	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3	coord: 9..196
None	No IPR available	CDD	cd02241	cupin_OxOx	coord: 23..198 e-value: 6.42558E-62 score: 188.959
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 60..197

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g02360.1	Moc02g02360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0048046	apoplast
molecular_function	GO:0030145	manganese ion binding