Homology
BLAST of Moc02g01030 vs. NCBI nr
Match:
XP_022148713.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])
HSP 1 Score: 1694.5 bits (4387), Expect = 0.0e+00
Identity = 835/932 (89.59%), Postives = 835/932 (89.59%), Query Frame = 0
Query: 64 MENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLE 123
MENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLE
Sbjct: 1 MENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLE 60
Query: 124 KGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVP 183
KGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVP
Sbjct: 61 KGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVP 120
Query: 184 YGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRES 243
YGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRES
Sbjct: 121 YGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRES 180
Query: 244 HHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYV 303
HHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYV
Sbjct: 181 HHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYV 240
Query: 304 PRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSA 363
PRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSA
Sbjct: 241 PRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSA 300
Query: 364 LLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPL 423
LLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPL
Sbjct: 301 LLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPL 360
Query: 424 ESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPL 483
ESKLDPAIYGPTKSAITNEIVEQQIKGFMTLN
Sbjct: 361 ESKLDPAIYGPTKSAITNEIVEQQIKGFMTLN---------------------------- 420
Query: 484 LTVDCPWFSLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLL 543
Sbjct: 421 ------------------------------------------------------------ 480
Query: 544 RSRSRMKEDEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLA 603
EAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLA
Sbjct: 481 ---------EAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLA 540
Query: 604 IELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEP 663
IELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEP
Sbjct: 541 IELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEP 600
Query: 664 YIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSM 723
YIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSM
Sbjct: 601 YIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSM 660
Query: 724 VYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEY 783
VYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEY
Sbjct: 661 VYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEY 720
Query: 784 VNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSG 843
VNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSG
Sbjct: 721 VNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSG 780
Query: 844 HHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATV 903
HHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATV
Sbjct: 781 HHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATV 835
Query: 904 LLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLK 963
LLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLK
Sbjct: 841 LLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLK 835
Query: 964 NRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 996
NRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI
Sbjct: 901 NRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 835
BLAST of Moc02g01030 vs. NCBI nr
Match:
XP_022148734.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])
HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 644/1000 (64.40%), Postives = 741/1000 (74.10%), Query Frame = 0
Query: 1 MLKSPVYNSSPHFHFVYRNRNPFVENNVEALLLKHPIPN---TPRLRGRFASASPSTAVK 60
MLKSPVYNS +Y R PF+ +N A PI P R SP++AVK
Sbjct: 1 MLKSPVYNSKQAL-CIY--RGPFLLSNNVAEPNLLPIGRQSVKPNAAPRLCFDSPNSAVK 60
Query: 61 ASVAAPMENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTL--VFELLSTE 120
+S A P NP SA VVVT+KR P++ D + W TKT V ELLS +
Sbjct: 61 SSAATPTGNPVSAAVVVTLKRPP----FPSI-------DGITHFWATKTKPPVLELLSAD 120
Query: 121 MDPTTGLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLK 180
+DPTTGLEKGTIK YA+KV RE+ + I Y+ + V IPA+FGAIGAVLVENQ+N ELFLK
Sbjct: 121 LDPTTGLEKGTIKVYAKKVAREDAE-VIMYDIKFV-IPADFGAIGAVLVENQHNKELFLK 180
Query: 181 DIVIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQG 240
DIVI G+P GP+RFSCNSWLSPKSHS DRRIFFTTKSYLPSNTP+GLKRFR ++L+ LQ
Sbjct: 181 DIVIQGLPTGPVRFSCNSWLSPKSHSGDRRIFFTTKSYLPSNTPDGLKRFRAKELRNLQD 240
Query: 241 NGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSE 300
NG GKRE+HHRIYDYDVYNDLGDPD GDE+KRP+LGGKQHPYPRRCRTGR+RS DPLSE
Sbjct: 241 NGSGKREAHHRIYDYDVYNDLGDPDNGDEYKRPVLGGKQHPYPRRCRTGRSRSPTDPLSE 300
Query: 301 VTSSNFYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSS 360
V S N YVPRDE F++ KQV A K V MLH +P LEA S +N L S
Sbjct: 301 VRSLNVYVPRDETFLEIKQVNMLA-KAVSWMLHCVVPTLEADTNLCSSHLSNTNNL---S 360
Query: 361 KYTWEPSALLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSI 420
K+TW + LL RL+DF KVV + L PSQ + DKFFWF+DEEFARQTLAGLNP SI
Sbjct: 361 KHTWNLNTLLRRLVDFVAHKVVDV-LPSPSQ-TFNGDKFFWFRDEEFARQTLAGLNPYSI 420
Query: 421 RLVTEWPLESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNG 480
RLVTEWP++SKLDP IYGP +SAIT+EIVE+QIKGFMTL
Sbjct: 421 RLVTEWPIKSKLDPCIYGPPESAITDEIVEEQIKGFMTL--------------------- 480
Query: 481 IFGQNIPLLTVDCPWFSLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGK 540
Sbjct: 481 ------------------------------------------------------------ 540
Query: 541 RMRSPSLLRSRSRMKEDEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNP 600
DEAV+QKKL+ LDYHDLFLPYV KVRK++GKTLYGSRTLFFLNP
Sbjct: 541 ----------------DEAVKQKKLFALDYHDLFLPYVTKVRKLKGKTLYGSRTLFFLNP 600
Query: 601 DNTLKPLAIELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWL 660
DNTLKPLAIELTRPP + K QWKDVF PC ++TGLWLWRIAKAHV+AHD GYHQLVSHWL
Sbjct: 601 DNTLKPLAIELTRPPTNAKPQWKDVFKPCCDATGLWLWRIAKAHVVAHDFGYHQLVSHWL 660
Query: 661 RTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGK 720
RTHCSVEPYIIA HRQLSAMHPIYRLLHPHFR T+EINA AR+ L NV GIIETAFS GK
Sbjct: 661 RTHCSVEPYIIATHRQLSAMHPIYRLLHPHFRGTIEINAAARKTLINVEGIIETAFSPGK 720
Query: 721 YSMEVSSMVYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDA 780
YSME++S+VYQKQWQFNLEALPADLI+RG+AVEDPNA HG+KL+IEDYPFAND LILW+A
Sbjct: 721 YSMEITSVVYQKQWQFNLEALPADLINRGLAVEDPNAPHGLKLTIEDYPFANDALILWEA 780
Query: 781 IKEWATEYVNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVS 840
IK+WATEYVNHYYP+P ++K D+ELQAWW EIR+VGHGDKK+EPWWPILNTP+DLIDVV+
Sbjct: 781 IKQWATEYVNHYYPEPNLIKLDEELQAWWMEIRTVGHGDKKDEPWWPILNTPKDLIDVVT 840
Query: 841 SMMWVTSGHHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTF 900
++MWVTSGHHAAVNFGQY+F YFP RPS AR++VPTE+PNS+LWK FLE+PE+VF DTF
Sbjct: 841 TIMWVTSGHHAAVNFGQYFFTRYFPIRPSFARVDVPTEDPNSRLWKYFLENPEKVFSDTF 881
Query: 901 PAHIQATVLLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQ 960
P H++A+VLL IL+ILSTH P+E+YLG +MEPAWG+D ++K+AF+KFSG+LKKLE+IID+
Sbjct: 901 PTHVEASVLLCILNILSTHPPDEKYLGGEMEPAWGEDAIIKEAFEKFSGKLKKLEEIIDE 881
Query: 961 RNANPKLKNRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 996
RN NPKLKNRHGAGV+PYQLLKPYSEPGVT +GVP SVSI
Sbjct: 961 RNGNPKLKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSVSI 881
BLAST of Moc02g01030 vs. NCBI nr
Match:
XP_022958149.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])
HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 614/996 (61.65%), Postives = 719/996 (72.19%), Query Frame = 0
Query: 1 MLKSPVYNSSPHFHFVYRNRNPFVENNVEALLLKHPIPNTPRLRGRFASASPSTAVKASV 60
ML+SPVY S + V+ L+L P P +P + VKASV
Sbjct: 1 MLRSPVYYSLSSSYQVH-------------LVLGRPYGIKPYT--TLTRCAPPSVVKASV 60
Query: 61 AAPMENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTT 120
APM++P SA VVTV R + L L N G+K+ L+D+TD LWG KTLV ELLS E+D T
Sbjct: 61 DAPMDHPLSAKAVVTVHRPLRGLSL-NFGIKQRLHDITD-LWGAKTLVLELLSAEIDSRT 120
Query: 121 GLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIH 180
GLEKGTIKGYA++V R+ +D ITYET+ +PAEFG+IGAVLVEN++N ELFLKDIV++
Sbjct: 121 GLEKGTIKGYAERVDRDGDD-VITYETKF-KVPAEFGSIGAVLVENEHNQELFLKDIVVY 180
Query: 181 GVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGK 240
G+P PLRFSCNSWL+PKS S D RIFFTTKSYLPSNTP+GLKR R E+LK LQGNG+GK
Sbjct: 181 GIPTAPLRFSCNSWLTPKSQSDDTRIFFTTKSYLPSNTPKGLKRIRAEELKKLQGNGYGK 240
Query: 241 RESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSN 300
RE+HHRIYDYDVYNDLGDPD+G EF+RP+LGGKQHPYPRRCRTGRAR Q D LSEV S +
Sbjct: 241 RETHHRIYDYDVYNDLGDPDKGVEFQRPVLGGKQHPYPRRCRTGRARCQTDSLSEVRSWD 300
Query: 301 FYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSS-KYTW 360
Y+PRDEAF + V T YS+L+ LE D + T + K W
Sbjct: 301 IYLPRDEAFSRIEYVRLLG-NTFYSLLNSL--KLETTITDTKFSITEELSNSNKGIKGIW 360
Query: 361 EPSALLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVT 420
+A+LP+L V K V IL L PS D DKFFWF+DEEFARQTLAGLNP SIRLV
Sbjct: 361 NFNAILPKLFSSVVTKAVDILTL-PSSETFDKDKFFWFRDEEFARQTLAGLNPYSIRLVK 420
Query: 421 EWPLESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQ 480
EWPL+S+L+P IYGP +SAIT+EIVE+QIKG MTL
Sbjct: 421 EWPLKSQLEPDIYGPPESAITDEIVERQIKGVMTL------------------------- 480
Query: 481 NIPLLTVDCPWFSLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRS 540
Sbjct: 481 ------------------------------------------------------------ 540
Query: 541 PSLLRSRSRMKEDEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTL 600
+EAV+QKKLYILDYHDL +PYVAKVRK++GKTLYGSRTLFFLNPD TL
Sbjct: 541 ------------EEAVKQKKLYILDYHDLLVPYVAKVRKLKGKTLYGSRTLFFLNPDGTL 600
Query: 601 KPLAIELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHC 660
+PLAIEL+RPP+DGK QWKDV TPC ++TGLWLW+I KAHVLAHDSG+HQLVSHWLRTHC
Sbjct: 601 RPLAIELSRPPMDGKAQWKDVLTPCWDATGLWLWKIGKAHVLAHDSGHHQLVSHWLRTHC 660
Query: 661 SVEPYIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSME 720
VEPYIIA HRQLSAMHPIYRLLHPHFRYTME NAIAR +L N GGIIET FS GKYSME
Sbjct: 661 CVEPYIIATHRQLSAMHPIYRLLHPHFRYTMETNAIARSSLINEGGIIETTFSPGKYSME 720
Query: 721 VSSMVYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEW 780
SS+ Y++ WQFNLEALPADLIHRG+AVEDPNA HG+KLSIEDYP+ANDGLILWDAIKEW
Sbjct: 721 FSSLAYRQHWQFNLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPYANDGLILWDAIKEW 780
Query: 781 ATEYVNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMW 840
A EYVNHYYPDP VVKSD+ELQAWWTEIR VGHGDKKEEPWWP+L+TP+DLID+V+++MW
Sbjct: 781 AMEYVNHYYPDPSVVKSDEELQAWWTEIREVGHGDKKEEPWWPVLDTPKDLIDIVTTIMW 840
Query: 841 VTSGHHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHI 900
+T+GHHA NFGQY FA YFP RPS+AR NVPTE+P K WK FLE PE VFL+TFP I
Sbjct: 841 ITTGHHAVANFGQYSFAKYFPNRPSIARANVPTEQP-KKHWKYFLEKPENVFLETFPTQI 875
Query: 901 QATVLLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNAN 960
QAT++ S+ S+L++H P+E+YLG+DME AW D+P + QAF+KFS +L+KLE IID+RN N
Sbjct: 901 QATIVSSVASVLASHPPDEKYLGKDMEAAWADEPNINQAFNKFSEKLRKLEKIIDERNVN 875
Query: 961 PKLKNRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 996
P+LKNRHGAG++PY+LLKP+SEPGVT +GVP S+SI
Sbjct: 961 PELKNRHGAGIEPYELLKPHSEPGVTGKGVPFSISI 875
BLAST of Moc02g01030 vs. NCBI nr
Match:
XP_022958530.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])
HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 610/984 (61.99%), Postives = 713/984 (72.46%), Query Frame = 0
Query: 15 FVYRNRNPFVENNVEALL-LKHPI--PNTPRLRGRFASASPSTAVKASVAAPMENPFSAH 74
F+Y + P + NV+A + L+H + P LR + +AV SV + ++
Sbjct: 18 FLY--QKPLLPGNVDACVSLQHSLTPPAASPLRFTSPNVIKPSAVHVSVPSIQSPVYTKA 77
Query: 75 VVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLEKGTIKGYA 134
VV TV+R + + L P G L+D+ + ++ L ELLS +MDPTTGLEKG +K Y
Sbjct: 78 VVTTVQRPIHQ-LFPKFGFIGRLHDIINR--RSRVLALELLSADMDPTTGLEKGRMKAYE 137
Query: 135 QKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVPYGPLRFSC 194
QKV R ED EI YE V IPA+FG IGAVLVEN + E+FLKDIVIHG+P GPL FSC
Sbjct: 138 QKVKR--EDDEIIYEISFV-IPADFGPIGAVLVENMHKKEVFLKDIVIHGIPTGPLHFSC 197
Query: 195 NSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRESHHRIYDYD 254
NSW++ K+ +DRRIFFTTKSYLPS TP+GLKR R E+L+ILQGNG+GKRE RIYDYD
Sbjct: 198 NSWITSKAQCNDRRIFFTTKSYLPSKTPDGLKRLRLEELRILQGNGYGKREKQERIYDYD 257
Query: 255 VYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYVPRDEAFID 314
VYNDLG+PD+GDE+KRP+LGGKQHPYPRRCRTGR R+Q DP SEV S FYVPRDEAF
Sbjct: 258 VYNDLGNPDKGDEYKRPVLGGKQHPYPRRCRTGRPRTQTDPSSEVKSLEFYVPRDEAFSI 317
Query: 315 TKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSALLPRLIDF 374
P K ++SML +PAL+ I D + SK+TW ++LLP L+
Sbjct: 318 PMIQENPLTKALHSMLSALLPALDTIGTDKQCSL---------SKHTWRFNSLLPTLMG- 377
Query: 375 AVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAI 434
ILLL SQ + DKFFWFKDEEFARQTLAGLNP SIRLVTEWPLESKL+P+I
Sbjct: 378 -------ILLLDSSQTSNNGDKFFWFKDEEFARQTLAGLNPYSIRLVTEWPLESKLEPSI 437
Query: 435 YGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWF 494
YG +SAIT+EIVEQQIKGFMT+
Sbjct: 438 YGSPESAITDEIVEQQIKGFMTI------------------------------------- 497
Query: 495 SLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKE 554
Sbjct: 498 ------------------------------------------------------------ 557
Query: 555 DEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPI 614
DEAV+Q+KL+ILDYHDLF+PYVAKVR+++G+TLYGSRTLFFLNPDNTL+PLAIEL+RPPI
Sbjct: 558 DEAVKQRKLFILDYHDLFIPYVAKVRELKGRTLYGSRTLFFLNPDNTLRPLAIELSRPPI 617
Query: 615 DGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQ 674
D K QWKDVFTPC ++ GLWLWRIAKAHVLAHDSG+HQLVSHWLRTHC VEPY+IA HRQ
Sbjct: 618 DDKPQWKDVFTPCWDAYGLWLWRIAKAHVLAHDSGHHQLVSHWLRTHCCVEPYVIATHRQ 677
Query: 675 LSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQF 734
LSAMHPIYRLLHPHFR+TM INA+ARE L NV G++E+ FS GKYSME+SS+VY+KQWQF
Sbjct: 678 LSAMHPIYRLLHPHFRHTMAINAVARETLINVEGLVESIFSPGKYSMEISSVVYEKQWQF 737
Query: 735 NLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDP 794
NLEALPADLIHRG+AVEDPNA HG+KLSIEDYPFANDGLILWDAIK+W TEYVNHYYPDP
Sbjct: 738 NLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPFANDGLILWDAIKQWVTEYVNHYYPDP 797
Query: 795 GVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFG 854
+V SD ELQAWWTEIR VGHGDKK+EPWWPILNTP DLI +V++++WVTSGHHAAVNFG
Sbjct: 798 SLVNSDGELQAWWTEIREVGHGDKKDEPWWPILNTPNDLIHIVTTIIWVTSGHHAAVNFG 857
Query: 855 QYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSIL 914
QY F SY+P RPS+ R+N+PTEEPNS L K FLE+PE VFLDTFP IQA +LL IL+IL
Sbjct: 858 QYPFTSYYPIRPSLTRLNIPTEEPNSTLRKYFLENPENVFLDTFPTQIQANILLFILNIL 879
Query: 915 STHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVD 974
S+HS +EEYLG DMEPAWGD+PV+K+AF+KFS +LKKLE+IID+RN NPKLKNRHGAGV
Sbjct: 918 SSHSRDEEYLGADMEPAWGDEPVIKEAFEKFSRKLKKLEEIIDERNGNPKLKNRHGAGVG 879
Query: 975 PYQLLKPYSEPGVTARGVPCSVSI 996
PYQ+LKPYSEPGVTARGVPCSVSI
Sbjct: 978 PYQILKPYSEPGVTARGVPCSVSI 879
BLAST of Moc02g01030 vs. NCBI nr
Match:
XP_022996082.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita maxima])
HSP 1 Score: 1203.7 bits (3113), Expect = 0.0e+00
Identity = 605/984 (61.48%), Postives = 713/984 (72.46%), Query Frame = 0
Query: 15 FVYRNRNPFVENNV-EALLLKHPIPNTPRLRGRFASAS--PSTAVKASVAAPMENPFSAH 74
F+Y + P++ N E + L+H + T RF S + +AV V + ++
Sbjct: 18 FLY--QKPWLPGNADECVSLQHSLTPTAASPLRFTSPNVIKPSAVHVPVPSIQTPVYTKA 77
Query: 75 VVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLEKGTIKGYA 134
VV TV+R + + L P G L+D+ D +K L ELLS +MDPTTGLEKG +K Y
Sbjct: 78 VVTTVQRPIHQ-LFPKFGFIGRLHDIIDR--RSKVLDLELLSADMDPTTGLEKGRMKAYE 137
Query: 135 QKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVPYGPLRFSC 194
+KV R ED EI YE V IPA+FG IGAVLVE+ + ++FLKDIVIHG+P GPL FSC
Sbjct: 138 EKVKR--EDDEIIYEISFV-IPADFGPIGAVLVESMHKKDVFLKDIVIHGIPTGPLHFSC 197
Query: 195 NSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRESHHRIYDYD 254
NSW++ K+ +DRRIFFTTKSYLPS TP+GLKR R ++L+ LQGNG+GKR+ H RIYDYD
Sbjct: 198 NSWITSKAQCNDRRIFFTTKSYLPSKTPDGLKRLRLQELRNLQGNGYGKRDKHQRIYDYD 257
Query: 255 VYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYVPRDEAFID 314
VYND+G+PD+G+E+KRP+LGGKQHPYPRRCRTGRAR+Q DP SEV S FYVPRDEAF
Sbjct: 258 VYNDIGNPDKGEEYKRPVLGGKQHPYPRRCRTGRARTQTDPSSEVKSPKFYVPRDEAFSI 317
Query: 315 TKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSALLPRLIDF 374
P K ++SML +PAL+ I+ + SK+TW+ ++ LP
Sbjct: 318 PMIQENPLTKALHSMLSALLPALDTISTHKQCNL---------SKHTWKFNSFLP----- 377
Query: 375 AVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAI 434
+ ILLL SQ + DKFFWFKDEEFARQTLAGLNP SIRLVTEWPL+SKL+P+I
Sbjct: 378 ---TPMGILLLDSSQISNNGDKFFWFKDEEFARQTLAGLNPYSIRLVTEWPLKSKLEPSI 437
Query: 435 YGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWF 494
YG +SAIT+EIVEQQIKGFMTL
Sbjct: 438 YGSPESAITDEIVEQQIKGFMTL------------------------------------- 497
Query: 495 SLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKE 554
Sbjct: 498 ------------------------------------------------------------ 557
Query: 555 DEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPI 614
DEAV+Q+KL+ILDYHDLF+PYVAKVR+++G+TLYGSRTLFFLNPDNTL+PLAIEL+RPPI
Sbjct: 558 DEAVKQRKLFILDYHDLFIPYVAKVRELKGRTLYGSRTLFFLNPDNTLRPLAIELSRPPI 617
Query: 615 DGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQ 674
D K QWKDVFTPC ++ GLWLWRIAKAHVLAHDSGYHQLVSHWLRTHC VEPY+IA HRQ
Sbjct: 618 DDKPQWKDVFTPCWDAYGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCCVEPYVIATHRQ 677
Query: 675 LSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQF 734
LSAMHPIYRLLHPHFR+TM INA+ARE L NV G+IE+ FS GKYSME+SS+VY+KQWQF
Sbjct: 678 LSAMHPIYRLLHPHFRHTMAINAVARETLINVEGLIESIFSPGKYSMEISSVVYEKQWQF 737
Query: 735 NLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDP 794
NLEALPADLIHRG+AVEDPNA HG+KLSIEDYPFANDGLILWDAIK+W TEYVNHYYPDP
Sbjct: 738 NLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPFANDGLILWDAIKQWVTEYVNHYYPDP 797
Query: 795 GVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFG 854
+V SD ELQAWWTEIR VGHGDKK+EPWWPILNTP DLI +V++++WVTSGHHAAVNFG
Sbjct: 798 SLVNSDGELQAWWTEIREVGHGDKKDEPWWPILNTPNDLIHIVTTIIWVTSGHHAAVNFG 857
Query: 855 QYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSIL 914
QY F SY+P RPS+ R+N+PTEEPNS LWK FLE+PE VFLDTFP IQA +LL IL+IL
Sbjct: 858 QYPFTSYYPIRPSLTRLNIPTEEPNSTLWKYFLENPENVFLDTFPTQIQANILLFILNIL 879
Query: 915 STHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVD 974
S+HS +EEYLG DMEPAWGD+PV+K+AF+KFS +LKKLE+IID+RN NPKLKNRHGAGV
Sbjct: 918 SSHSRDEEYLGADMEPAWGDEPVIKEAFEKFSRKLKKLEEIIDERNGNPKLKNRHGAGVG 879
Query: 975 PYQLLKPYSEPGVTARGVPCSVSI 996
PYQ+LKPYSEPGVTARGVPCSVSI
Sbjct: 978 PYQILKPYSEPGVTARGVPCSVSI 879
BLAST of Moc02g01030 vs. ExPASy Swiss-Prot
Match:
O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)
HSP 1 Score: 980.7 bits (2534), Expect = 1.2e-284
Identity = 498/969 (51.39%), Postives = 644/969 (66.46%), Query Frame = 0
Query: 39 NTPRLRGRFASASPSTAVKASVAAPMENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVT 98
N R+ + AS + AV +S E VVTV++ V NL L R L D+
Sbjct: 45 NNFRVHHNYNGASTTKAVLSST----EKATGVKAVVTVQKQV------NLNLSRGLDDIG 104
Query: 99 DYLWGTKTLVFELLSTEMDPTTGLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGA 158
D L K+L+ +++ E+D TG+EK I+ YA + GR + DG+ YE + V IP +FG
Sbjct: 105 DLL--GKSLLLWIVAAELDHKTGIEKPGIRAYAHR-GR-DVDGDTHYEADFV-IPQDFGE 164
Query: 159 IGAVLVENQYNTELFLKDIVIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNT 218
+GA+L+EN+++ E+++K+IVI G +G + +CNSW+ K + D+RIFFT KSYLPS T
Sbjct: 165 VGAILIENEHHKEMYVKNIVIDGFVHGKVEITCNSWVHSKFDNPDKRIFFTNKSYLPSQT 224
Query: 219 PEGLKRFREEDLKILQGNGHGKRESHHRIYDYDVYNDLGDPD-RGDEFKRPILGGKQHPY 278
P G+ R REE+L L+G+G G+R+ RIYDYDVYNDLG+ D D+ KRP+LGGK+ PY
Sbjct: 225 PSGVSRLREEELVTLRGDGIGERKVFERIYDYDVYNDLGEADSNNDDAKRPVLGGKELPY 284
Query: 279 PRRCRTGRARSQADPLSEVTSSNFYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAI 338
PRRC+TGR RS+ DPLSE S+ YVPRDEAF + K V TVYS+LH +PALE++
Sbjct: 285 PRRCKTGRPRSKKDPLSETRSTFVYVPRDEAFSEVKSVAFSG-NTVYSVLHAVVPALESV 344
Query: 339 AQD----------IESRFTNRVMLLPSSKYTWEPSALLPRLIDFAVDKVVRILLLGPSQA 398
D I+S F V L ++PRLI A+ + +LL S
Sbjct: 345 VTDPNLGFPHFPAIDSLFNVGVDLPGLGDKKSGLFNVVPRLIK-AISDTRKDVLLFESPQ 404
Query: 399 VLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAIYGPTKSAITNEIVEQQ 458
++ DKF WF+D EFARQTLAGLNP SIRLVTEWPL SKLDP +YGP +S IT E++E++
Sbjct: 405 LVQRDKFSWFRDVEFARQTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKELIEKE 464
Query: 459 IKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWFSLFLSLLFLSHLRPAA 518
I +MT+
Sbjct: 465 IGNYMTV----------------------------------------------------- 524
Query: 519 TVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKEDEAVEQKKLYILDYHD 578
++AV+QKKL+ILDYHD
Sbjct: 525 --------------------------------------------EQAVQQKKLFILDYHD 584
Query: 579 LFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQWKDVFTP-CLN 638
L LPYV KV +++G LYGSRT+FFL P TLKPLAIELTRPP+D K QWK+V++P N
Sbjct: 585 LLLPYVNKVNELKGSMLYGSRTIFFLTPQGTLKPLAIELTRPPVDDKPQWKEVYSPNDWN 644
Query: 639 STGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMHPIYRLLHPHF 698
+TG WLW++AKAHVL+HDSGYHQLVSHWLRTHC EPYIIA +RQLSAMHPIYRLLHPHF
Sbjct: 645 ATGAWLWKLAKAHVLSHDSGYHQLVSHWLRTHCCTEPYIIASNRQLSAMHPIYRLLHPHF 704
Query: 699 RYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEALPADLIHRGMA 758
RYTMEINA+AREAL N G+IE++F GKY++E+SS+ Y +W+F+ EALP +LI RG+A
Sbjct: 705 RYTMEINALAREALINANGVIESSFFPGKYAIELSSIAYGAEWRFDQEALPQNLISRGLA 764
Query: 759 VEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKSDKELQAWWTE 818
VEDPN HG+KL+IEDYPFANDGL+LWD +K+W T YVNHYYP +++SDKELQAWW+E
Sbjct: 765 VEDPNEPHGLKLAIEDYPFANDGLVLWDILKQWVTNYVNHYYPQTNLIESDKELQAWWSE 824
Query: 819 IRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFASYFPARPSVA 878
I++VGHGDK++EPWWP L TP DLI ++++++WVTSGHHAAVNFGQY +A YFP RP+VA
Sbjct: 825 IKNVGHGDKRDEPWWPELKTPNDLIGIITTIVWVTSGHHAAVNFGQYSYAGYFPNRPTVA 884
Query: 879 RINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSPEEEYLGEDME 938
R +PTE+P ++ W+ F+ PE L FP+ IQAT +++IL +LS HSP+EEY+GE +E
Sbjct: 885 RSKMPTEDPTAEEWEWFMNKPEEALLRCFPSQIQATKVMAILDVLSNHSPDEEYIGEKIE 899
Query: 939 PAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVDPYQLLKPYSEPGVTA 996
P W +DPV+ AF+ FSG+LK+LE IID RN + KL NR+GAGV PY+LLKPYSEPGVT
Sbjct: 945 PYWAEDPVINAAFEVFSGKLKELEGIIDARNNDSKLSNRNGAGVMPYELLKPYSEPGVTG 899
BLAST of Moc02g01030 vs. ExPASy Swiss-Prot
Match:
R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)
HSP 1 Score: 934.5 bits (2414), Expect = 1.0e-270
Identity = 481/954 (50.42%), Postives = 626/954 (65.62%), Query Frame = 0
Query: 53 STAVKASVAAPMENPFSAHVVVTVKRHV-GRLLLPNLGLKRWLYD-VTDYLWGTKTLVFE 112
S+ +KA + + VVTV+ + G L +GL + D V+D+L ++ + E
Sbjct: 53 SSNIKAIMPDSIGKSIRKKCVVTVQPTISGALTAVTVGLLGTVADSVSDFL--GRSFLLE 112
Query: 113 LLSTEMDPTTGLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNT 172
L+S+++D ++G EK T+K YA ++E Y+ E +P +FG IGAVLV+N+ +
Sbjct: 113 LVSSDLD-SSGKEKDTVKAYATYDELDKESKLYKYQCEF-EVPDDFGEIGAVLVQNERHR 172
Query: 173 ELFLKDIVIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDL 232
+ ++K+IV+ + + F+C+SW+ K + D+RIFF KSYLPS TPEGLK R++DL
Sbjct: 173 DAYVKNIVLDEI----VTFTCDSWIHSKFDNPDKRIFFLNKSYLPSETPEGLKSLRQKDL 232
Query: 233 KILQGNGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQA 292
+ L+GNG G+R+S RIYDYD YND+GDPD + RP+LGG +HP+PRRCRTGR +
Sbjct: 233 ESLRGNGEGERQSFDRIYDYDTYNDIGDPDTDSDMARPVLGGNEHPFPRRCRTGRKMTST 292
Query: 293 DPLSEV-TSSNFYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFT--N 352
+P SE T+ FYVPRDE F + KQ+T A T+YS+LHG IPAL ++ +D + F
Sbjct: 293 EPWSESRTTLPFYVPRDEDFAEIKQITRGAT-TLYSVLHGVIPALSSVLKDEDKGFPLFR 352
Query: 353 RVMLLPSSKYTWEPS------ALLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEE 412
+ LL +P + LPRL+ A+ + +L + + D F WF+DEE
Sbjct: 353 DIELLYEKGVDIDPPPDSGTLSALPRLVK-AITNSTKKVLQFETPRIKHKDSFSWFRDEE 412
Query: 413 FARQTLAGLNPCSIRLVTEWPLESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNECRFDG 472
F RQTLAGLNP SI+LVTEWPL SKLDP +YGP +SAIT E VE++IKGFMT
Sbjct: 413 FCRQTLAGLNPYSIQLVTEWPLMSKLDPEVYGPAESAITKETVEEEIKGFMT-------- 472
Query: 473 FGVGVYLIPKFQNGIFGQNIPLLTVDCPWFSLFLSLLFLSHLRPAATVDQPSAVAALSPL 532
Sbjct: 473 ------------------------------------------------------------ 532
Query: 533 HYHRRALSFAIVGKRMRSPSLLRSRSRMKEDEAVEQKKLYILDYHDLFLPYVAKVRKIEG 592
+EA+EQK+L++LDYHDL LPYV KVR+IEG
Sbjct: 533 -----------------------------XEEALEQKRLFLLDYHDLLLPYVNKVREIEG 592
Query: 593 KTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVL 652
TLYGSRTL FL TL+PLAIELTRPP +GK QWK V+TPC ++T WLW++AKAHVL
Sbjct: 593 TTLYGSRTLMFLTCTGTLRPLAIELTRPPNNGKPQWKHVYTPCWDATDXWLWKLAKAHVL 652
Query: 653 AHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALT 712
AHDSGYHQLVSHWLRTHC EPYIIA +RQLS MHPI RLL PH RYTM+IN +AR +L
Sbjct: 653 AHDSGYHQLVSHWLRTHCVTEPYIIATNRQLSKMHPIQRLLCPHLRYTMQINGLARLSLI 712
Query: 713 NVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIE 772
N GIIE++FS KYSM++SS Y ++W+F+ EALPADLI RGMAVED +A HG+KL+IE
Sbjct: 713 NANGIIESSFSPRKYSMQLSSDAYAQKWRFDHEALPADLISRGMAVEDESAPHGIKLTIE 772
Query: 773 DYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWW 832
DYPFANDGL+LWDAIK+WAT Y+NHYYP +V+SD+ELQAWWTEIR+VGH DKK+EPWW
Sbjct: 773 DYPFANDGLLLWDAIKQWATAYINHYYPQAKLVESDEELQAWWTEIRTVGHADKKDEPWW 832
Query: 833 PILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWK 892
P L T +DLI VVS++MWV+SGHH+AVNFGQY F YFP RP++AR +P E+P + W+
Sbjct: 833 PQLKTQQDLIGVVSTIMWVSSGHHSAVNFGQYDFGGYFPNRPTIARTKMPNEDPTFEEWE 892
Query: 893 RFLEDPERVFLDTFPAHIQATVLLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDK 952
F+E PE V L+ FP IQAT +++IL +LS+HSP+EEY+G ME +W +P +K AF++
Sbjct: 893 AFMEKPEDVLLNCFPTQIQATKVMAILDVLSSHSPDEEYIGTSMEASWEAEPAIKSAFEE 899
Query: 953 FSGRLKKLEDIIDQRNANPKLKNRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 996
F GRLKKL+DIID RN +P L+NR GAG+ YQLLKP+S GVT +GVP S+SI
Sbjct: 953 FCGRLKKLDDIIDSRNRDPILRNRTGAGLVQYQLLKPFSGHGVTGKGVPYSISI 899
BLAST of Moc02g01030 vs. ExPASy Swiss-Prot
Match:
P38418 (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1)
HSP 1 Score: 867.1 bits (2239), Expect = 2.0e-250
Identity = 446/925 (48.22%), Postives = 584/925 (63.14%), Query Frame = 0
Query: 85 LPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLEKGTIKGYAQKVGREEEDGEIT 144
L + R L D+ D ++L+ EL+S + D ++ T++ YAQ+V E D +
Sbjct: 86 LEGITWSRGLDDIADI--RGRSLLVELISAKTD-----QRITVEDYAQRVWAEAPDEK-- 145
Query: 145 YETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVPYGPLRFSCNSWLSPKSHSHDR 204
YE E +P +FG +GA+ ++NQY+ +LFLK + + +P G + F+C SW++PKS +
Sbjct: 146 YECEF-EMPEDFGPVGAIKIQNQYHRQLFLKGVELK-LPGGSITFTCESWVAPKSVDPTK 205
Query: 205 RIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGH---GKRESHHRIYDYDVYNDLGDPDR 264
RIFF+ KSYLPS TPE LK++R+E+L+ LQG G+ RIYDYDVYND+GDPD
Sbjct: 206 RIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTKFERIYDYDVYNDVGDPDN 265
Query: 265 GDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVT-SSNFYVPRDEAFIDTKQVTTPAV 324
E RP++GG HPYPRRC+TGR + DP SE FYVPRDE F T + T+
Sbjct: 266 DPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEF-STAKGTSFTG 325
Query: 325 KTVYSMLHGFIPALEAIA----------QDIESRFTNRVMLLPSSKYTWEPSALLPRLID 384
K V + L P +E++ + I++ F + L + LLPR+I
Sbjct: 326 KAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKDAGLL----PLLPRIIK 385
Query: 385 FAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPA 444
A+ + +L + +++ D+F W +D+EFARQTLAGLNP SI+LV EWPL SKLDPA
Sbjct: 386 -ALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPA 445
Query: 445 IYGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPW 504
+YG S IT EIVE+++KG MT+
Sbjct: 446 VYGDPTSLITWEIVEREVKGNMTV------------------------------------ 505
Query: 505 FSLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMK 564
Sbjct: 506 ------------------------------------------------------------ 565
Query: 565 EDEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPP 624
DEA++ K+L++LDYHDL LPYV KVR++ TLY SRTLFFL+ D+TL+P+AIELT PP
Sbjct: 566 -DEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPP 625
Query: 625 IDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHR 684
K QWK VFTP ++T WLW +AK H ++HD+GYHQL+SHWLRTH EPYIIA +R
Sbjct: 626 NINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANR 685
Query: 685 QLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQ 744
QLSAMHPIYRLLHPHFRYTMEINA AR++L N GGIIET F GKY++E+SS VY K W+
Sbjct: 686 QLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWR 745
Query: 745 FNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPD 804
F+ E LPADLI RG+A ED A HGV+L+I DYPFANDGLILWDAIKEW T+YV HYYPD
Sbjct: 746 FDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPD 805
Query: 805 PGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNF 864
++ SD+ELQ WW+E+R++GHGDKK+EPWWP+L T +DLI VV+++ WVTSGHHAAVNF
Sbjct: 806 EELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNF 865
Query: 865 GQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSI 924
GQY + YFP RP+ RI +PTE+P + K F E PE+V L T+P+ QAT+++ L +
Sbjct: 866 GQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDL 896
Query: 925 LSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGV 984
LSTHSP+EEY+GE E +W ++PV+ AF++F G+L+ LE +ID+RN N LKNR GAGV
Sbjct: 926 LSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGV 896
Query: 985 DPYQLLKPYSEPGVTARGVPCSVSI 996
Y+LLKP SE GVT GVP S+SI
Sbjct: 986 VKYELLKPTSEHGVTGMGVPYSISI 896
BLAST of Moc02g01030 vs. ExPASy Swiss-Prot
Match:
Q8GSM2 (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1)
HSP 1 Score: 859.0 bits (2218), Expect = 5.3e-248
Identity = 447/965 (46.32%), Postives = 609/965 (63.11%), Query Frame = 0
Query: 46 RFASASPSTAVKASVAAPMENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTK 105
R + AS AV S + + +TV V + ++ + R L D+ D L+G K
Sbjct: 47 RISCASTEEAVGVSTSVTTKER-----ALTVTAIVTAQVPTSVYVARGLDDIQD-LFG-K 106
Query: 106 TLVFELLSTEMDPTTGLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVE 165
TL+ EL+S+E+DP TG E+ +KG+A +E TYE ++ ++PA FG +GAVLVE
Sbjct: 107 TLLLELVSSELDPKTGRERERVKGFAHMTLKEG-----TYEAKM-SVPASFGPVGAVLVE 166
Query: 166 NQYNTELFLKDI--VIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLK 225
N+++ E+F+KDI + G + F SW+ K + R FFT KSYLPS TP G++
Sbjct: 167 NEHHREMFIKDIKLITGGDESTAITFDVASWVHSKFDDPEPRAFFTVKSYLPSQTPPGIE 226
Query: 226 RFREEDLKILQGNGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRT 285
R+++L+ L+G+GH +R+ H R+YDYD YNDLGDPD+ + KRP+LG K+HPYPRRCRT
Sbjct: 227 ALRKKELETLRGDGHSERKFHERVYDYDTYNDLGDPDKNIDHKRPVLGTKEHPYPRRCRT 286
Query: 286 GRARSQADPLSEVTSSNFYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQD--- 345
GR ++ DP +E SS YVPRDE F D K T A T+ S LH +PA+ + +
Sbjct: 287 GRPKTLYDPETETRSSPVYVPRDEQFSDVKGRTFSAT-TLRSGLHAILPAVAPLLNNSHG 346
Query: 346 ------IESRFTNRVMLLPSSKYTWEP----SALLPRLIDFAVDKVVRILLLGPSQAVLD 405
I++ +++ + LP + + ++PR++ D +L + +L+
Sbjct: 347 FSHFPAIDALYSDGIP-LPVDGHGGNSFNVINDVIPRVVQMIEDTTEHVLRFEVPE-MLE 406
Query: 406 SDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAIYGPTKSAITNEIVEQQIKG 465
D+F WF+DEEFARQTLAGLNP IR +TE+P+ SKLDPA+YGP +SA++ EI+E+ + G
Sbjct: 407 RDRFSWFRDEEFARQTLAGLNPICIRRLTEFPIVSKLDPAVYGPAESALSKEILEKMMNG 466
Query: 466 FMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWFSLFLSLLFLSHLRPAATVD 525
MT+
Sbjct: 467 RMTV-------------------------------------------------------- 526
Query: 526 QPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKEDEAVEQKKLYILDYHDLFL 585
+EA+E+K+L++LDYHD+FL
Sbjct: 527 -----------------------------------------EEAMEKKRLFLLDYHDVFL 586
Query: 586 PYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQWKDVFTPCLNSTGL 645
PYV +VR++ TLYGSRT+FFL+ + TL PLAIELTRP K QWK FT ++T
Sbjct: 587 PYVHRVRELPDTTLYGSRTVFFLSDEGTLMPLAIELTRPQSPTKPQWKRAFTHGSDATES 646
Query: 646 WLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFRYTM 705
WLW++AKAHVL HD+GYHQLVSHWLRTH VEPYIIA +RQLS MHP+YRLLHPHFRYTM
Sbjct: 647 WLWKLAKAHVLTHDTGYHQLVSHWLRTHACVEPYIIATNRQLSRMHPVYRLLHPHFRYTM 706
Query: 706 EINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEALPADLIHRGMAVEDP 765
EINA+AREAL N GIIE AF GKYS+E+SS+ Y WQFN EALP DLI+RG+AV
Sbjct: 707 EINALAREALINADGIIEEAFLAGKYSIELSSVAYGAAWQFNTEALPEDLINRGLAVRRD 766
Query: 766 NAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKSDKELQAWWTEIRSV 825
+ ++L+I+DYP+A+DGL++W +IK+WA++YV+ YY G V D+EL+AWW E+R+
Sbjct: 767 DGE--LELAIKDYPYADDGLLIWGSIKQWASDYVDFYYKSDGDVAGDEELRAWWEEVRTK 826
Query: 826 GHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFASYFPARPSVARINV 885
GH DKK+EPWWP+ +T E+L+ +++ +MWVTSGHHAAVNFGQY++A YFP RP+V R N+
Sbjct: 827 GHADKKDEPWWPVCDTKENLVQILTIIMWVTSGHHAAVNFGQYHYAGYFPNRPTVVRRNI 886
Query: 886 PTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSPEEEYLGEDMEPAWG 945
P EE K+F+ PE V L + P+ +QA +++ L ILS+HSP+EEY+GE EPAW
Sbjct: 887 PVEENRDDEMKKFMARPEEVLLQSLPSQMQAIKVMATLDILSSHSPDEEYMGEYAEPAWL 896
Query: 946 DDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVDPYQLLKPYSEPGVTARGVP 996
+P+VK AF+KFSGRLK+ E ID RN NP+ KNR GAG+ PY+LLKP+SEPGVT RG+P
Sbjct: 947 AEPMVKAAFEKFSGRLKEAEGTIDMRNNNPENKNRCGAGIVPYELLKPFSEPGVTGRGIP 896
BLAST of Moc02g01030 vs. ExPASy Swiss-Prot
Match:
Q84YK8 (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)
HSP 1 Score: 839.3 bits (2167), Expect = 4.4e-242
Identity = 428/914 (46.83%), Postives = 579/914 (63.35%), Query Frame = 0
Query: 105 KTLVFELLSTEMDPTTGLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLV 164
++L EL+S+E++ TG +K T+ YA KV +++ G +TYE + ++P FG IGAV+V
Sbjct: 130 RSLFLELVSSELEAKTGKKKATVHSYAHKVD-DDDHGVVTYEADF-DVPTGFGPIGAVVV 189
Query: 165 ENQYNTELFLKDI-VIHGVPYG---PLRFSCNSWLSPKSHSHD----RRIFFTTKSYLPS 224
N+ E+FL+D+ + G G L CNSW+ PKS + +RIFF K+YLP
Sbjct: 190 TNELGQEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQPKSSIDEGTPGKRIFF-AKAYLPG 249
Query: 225 NTPEGLKRFREEDLKILQGNGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGG-KQH 284
TP GL+ +REEDLK +GNG G+RE+ R+YDYDVYNDLG+PD + RP+LGG KQ
Sbjct: 250 QTPAGLRSYREEDLKQKRGNGAGQREADDRVYDYDVYNDLGNPDSNGDLARPVLGGSKQF 309
Query: 285 PYPRRCRTGRARSQADPLSEVTSSNFYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALE 344
PYPRRCRTGR S+ DP SE N YVPRDE F + K +KT+ S+LH +PA +
Sbjct: 310 PYPRRCRTGRPPSKKDPKSETRKGNVYVPRDEEFSEVKNAQF-LLKTLQSVLHAAVPAAQ 369
Query: 345 AIAQD-------------IESRFTNRVMLLPSSKYTWEPSALLPRLIDFAVDKVVRILLL 404
+ D I+ F + V LP + +++PRL++ D +LL
Sbjct: 370 SALIDNLSLNLPFPSFFVIDKLFEDGVE-LPGVEKLGFLHSIVPRLLELLRDSPGDKILL 429
Query: 405 GPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAIYGPTKSAITNE 464
+ A + DKF W +DEEFAR+TLAG+NP +I LV E+PL+SKLDPA+YGP +SAIT +
Sbjct: 430 FDTPANVQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITAD 489
Query: 465 IVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWFSLFLSLLFLSH 524
++E+Q++ MT+
Sbjct: 490 LLEEQMRRVMTV------------------------------------------------ 549
Query: 525 LRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKEDEAVEQKKLYI 584
+EA+ QK+L++
Sbjct: 550 -------------------------------------------------EEAISQKRLFM 609
Query: 585 LDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQWKDVFT 644
LD+HDLFLPYV K+R ++ T+YGSRT+FFL D TL+ LAIELTRP + QW+ VFT
Sbjct: 610 LDFHDLFLPYVHKIRSLKHTTMYGSRTIFFLTDDGTLRLLAIELTRPASPSQPQWRQVFT 669
Query: 645 PCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMHPIYRLL 704
P ++T WLWR+AKAHV AHD+G+H+L++HWLRTHC+VEPYIIA +RQLS MHPIY+LL
Sbjct: 670 PSTDTTKSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLL 729
Query: 705 HPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEALPADLIH 764
HPHFRYTM INA+AR L + GIIE +FS KYSME+SS+ Y K W+F++EALPADL+
Sbjct: 730 HPHFRYTMRINALARSRLISAAGIIELSFSPQKYSMELSSVAYDKLWRFDMEALPADLVR 789
Query: 765 RGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKSDKELQA 824
RGMA EDP A HG++L+IEDYPFANDGL++WDAIK W YV +YPD V D+ELQA
Sbjct: 790 RGMAEEDPTAEHGLRLAIEDYPFANDGLLIWDAIKTWVQAYVARFYPDADSVAGDEELQA 849
Query: 825 WWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFASYFPAR 884
+WTE+R+ GHGDKK+ PWWP L++PE L +++++WV + HHAAVNFGQY F YFP R
Sbjct: 850 FWTEVRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNR 909
Query: 885 PSVARINVPTEEP-NSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSPEEEYL 944
PS+AR +P EEP + +RFL++P++ + FP+ +QATV++++L +LSTHS +EEYL
Sbjct: 910 PSIARTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQATVVMAVLDVLSTHSTDEEYL 941
Query: 945 GEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVDPYQLLKPYSE 996
G + W D V+ A+ F+ RLK++E +ID RN + KLKNR GAG+ PYQL+KP+S+
Sbjct: 970 GGEQTRPWNSDAAVQAAYAGFTARLKEIEGVIDGRNKDRKLKNRCGAGILPYQLMKPFSD 941
BLAST of Moc02g01030 vs. ExPASy TrEMBL
Match:
A0A6J1D4V6 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017309 PE=3 SV=1)
HSP 1 Score: 1694.5 bits (4387), Expect = 0.0e+00
Identity = 835/932 (89.59%), Postives = 835/932 (89.59%), Query Frame = 0
Query: 64 MENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLE 123
MENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLE
Sbjct: 1 MENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLE 60
Query: 124 KGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVP 183
KGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVP
Sbjct: 61 KGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVP 120
Query: 184 YGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRES 243
YGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRES
Sbjct: 121 YGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRES 180
Query: 244 HHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYV 303
HHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYV
Sbjct: 181 HHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYV 240
Query: 304 PRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSA 363
PRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSA
Sbjct: 241 PRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSA 300
Query: 364 LLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPL 423
LLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPL
Sbjct: 301 LLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPL 360
Query: 424 ESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPL 483
ESKLDPAIYGPTKSAITNEIVEQQIKGFMTLN
Sbjct: 361 ESKLDPAIYGPTKSAITNEIVEQQIKGFMTLN---------------------------- 420
Query: 484 LTVDCPWFSLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLL 543
Sbjct: 421 ------------------------------------------------------------ 480
Query: 544 RSRSRMKEDEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLA 603
EAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLA
Sbjct: 481 ---------EAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLA 540
Query: 604 IELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEP 663
IELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEP
Sbjct: 541 IELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEP 600
Query: 664 YIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSM 723
YIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSM
Sbjct: 601 YIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSM 660
Query: 724 VYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEY 783
VYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEY
Sbjct: 661 VYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEY 720
Query: 784 VNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSG 843
VNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSG
Sbjct: 721 VNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSG 780
Query: 844 HHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATV 903
HHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATV
Sbjct: 781 HHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATV 835
Query: 904 LLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLK 963
LLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLK
Sbjct: 841 LLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLK 835
Query: 964 NRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 996
NRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI
Sbjct: 901 NRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 835
BLAST of Moc02g01030 vs. ExPASy TrEMBL
Match:
A0A6J1D4V5 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017327 PE=3 SV=1)
HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 644/1000 (64.40%), Postives = 741/1000 (74.10%), Query Frame = 0
Query: 1 MLKSPVYNSSPHFHFVYRNRNPFVENNVEALLLKHPIPN---TPRLRGRFASASPSTAVK 60
MLKSPVYNS +Y R PF+ +N A PI P R SP++AVK
Sbjct: 1 MLKSPVYNSKQAL-CIY--RGPFLLSNNVAEPNLLPIGRQSVKPNAAPRLCFDSPNSAVK 60
Query: 61 ASVAAPMENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTL--VFELLSTE 120
+S A P NP SA VVVT+KR P++ D + W TKT V ELLS +
Sbjct: 61 SSAATPTGNPVSAAVVVTLKRPP----FPSI-------DGITHFWATKTKPPVLELLSAD 120
Query: 121 MDPTTGLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLK 180
+DPTTGLEKGTIK YA+KV RE+ + I Y+ + V IPA+FGAIGAVLVENQ+N ELFLK
Sbjct: 121 LDPTTGLEKGTIKVYAKKVAREDAE-VIMYDIKFV-IPADFGAIGAVLVENQHNKELFLK 180
Query: 181 DIVIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQG 240
DIVI G+P GP+RFSCNSWLSPKSHS DRRIFFTTKSYLPSNTP+GLKRFR ++L+ LQ
Sbjct: 181 DIVIQGLPTGPVRFSCNSWLSPKSHSGDRRIFFTTKSYLPSNTPDGLKRFRAKELRNLQD 240
Query: 241 NGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSE 300
NG GKRE+HHRIYDYDVYNDLGDPD GDE+KRP+LGGKQHPYPRRCRTGR+RS DPLSE
Sbjct: 241 NGSGKREAHHRIYDYDVYNDLGDPDNGDEYKRPVLGGKQHPYPRRCRTGRSRSPTDPLSE 300
Query: 301 VTSSNFYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSS 360
V S N YVPRDE F++ KQV A K V MLH +P LEA S +N L S
Sbjct: 301 VRSLNVYVPRDETFLEIKQVNMLA-KAVSWMLHCVVPTLEADTNLCSSHLSNTNNL---S 360
Query: 361 KYTWEPSALLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSI 420
K+TW + LL RL+DF KVV + L PSQ + DKFFWF+DEEFARQTLAGLNP SI
Sbjct: 361 KHTWNLNTLLRRLVDFVAHKVVDV-LPSPSQ-TFNGDKFFWFRDEEFARQTLAGLNPYSI 420
Query: 421 RLVTEWPLESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNG 480
RLVTEWP++SKLDP IYGP +SAIT+EIVE+QIKGFMTL
Sbjct: 421 RLVTEWPIKSKLDPCIYGPPESAITDEIVEEQIKGFMTL--------------------- 480
Query: 481 IFGQNIPLLTVDCPWFSLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGK 540
Sbjct: 481 ------------------------------------------------------------ 540
Query: 541 RMRSPSLLRSRSRMKEDEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNP 600
DEAV+QKKL+ LDYHDLFLPYV KVRK++GKTLYGSRTLFFLNP
Sbjct: 541 ----------------DEAVKQKKLFALDYHDLFLPYVTKVRKLKGKTLYGSRTLFFLNP 600
Query: 601 DNTLKPLAIELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWL 660
DNTLKPLAIELTRPP + K QWKDVF PC ++TGLWLWRIAKAHV+AHD GYHQLVSHWL
Sbjct: 601 DNTLKPLAIELTRPPTNAKPQWKDVFKPCCDATGLWLWRIAKAHVVAHDFGYHQLVSHWL 660
Query: 661 RTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGK 720
RTHCSVEPYIIA HRQLSAMHPIYRLLHPHFR T+EINA AR+ L NV GIIETAFS GK
Sbjct: 661 RTHCSVEPYIIATHRQLSAMHPIYRLLHPHFRGTIEINAAARKTLINVEGIIETAFSPGK 720
Query: 721 YSMEVSSMVYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDA 780
YSME++S+VYQKQWQFNLEALPADLI+RG+AVEDPNA HG+KL+IEDYPFAND LILW+A
Sbjct: 721 YSMEITSVVYQKQWQFNLEALPADLINRGLAVEDPNAPHGLKLTIEDYPFANDALILWEA 780
Query: 781 IKEWATEYVNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVS 840
IK+WATEYVNHYYP+P ++K D+ELQAWW EIR+VGHGDKK+EPWWPILNTP+DLIDVV+
Sbjct: 781 IKQWATEYVNHYYPEPNLIKLDEELQAWWMEIRTVGHGDKKDEPWWPILNTPKDLIDVVT 840
Query: 841 SMMWVTSGHHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTF 900
++MWVTSGHHAAVNFGQY+F YFP RPS AR++VPTE+PNS+LWK FLE+PE+VF DTF
Sbjct: 841 TIMWVTSGHHAAVNFGQYFFTRYFPIRPSFARVDVPTEDPNSRLWKYFLENPEKVFSDTF 881
Query: 901 PAHIQATVLLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQ 960
P H++A+VLL IL+ILSTH P+E+YLG +MEPAWG+D ++K+AF+KFSG+LKKLE+IID+
Sbjct: 901 PTHVEASVLLCILNILSTHPPDEKYLGGEMEPAWGEDAIIKEAFEKFSGKLKKLEEIIDE 881
Query: 961 RNANPKLKNRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 996
RN NPKLKNRHGAGV+PYQLLKPYSEPGVT +GVP SVSI
Sbjct: 961 RNGNPKLKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSVSI 881
BLAST of Moc02g01030 vs. ExPASy TrEMBL
Match:
A0A6J1H492 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459464 PE=3 SV=1)
HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 614/996 (61.65%), Postives = 719/996 (72.19%), Query Frame = 0
Query: 1 MLKSPVYNSSPHFHFVYRNRNPFVENNVEALLLKHPIPNTPRLRGRFASASPSTAVKASV 60
ML+SPVY S + V+ L+L P P +P + VKASV
Sbjct: 1 MLRSPVYYSLSSSYQVH-------------LVLGRPYGIKPYT--TLTRCAPPSVVKASV 60
Query: 61 AAPMENPFSAHVVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTT 120
APM++P SA VVTV R + L L N G+K+ L+D+TD LWG KTLV ELLS E+D T
Sbjct: 61 DAPMDHPLSAKAVVTVHRPLRGLSL-NFGIKQRLHDITD-LWGAKTLVLELLSAEIDSRT 120
Query: 121 GLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIH 180
GLEKGTIKGYA++V R+ +D ITYET+ +PAEFG+IGAVLVEN++N ELFLKDIV++
Sbjct: 121 GLEKGTIKGYAERVDRDGDD-VITYETKF-KVPAEFGSIGAVLVENEHNQELFLKDIVVY 180
Query: 181 GVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGK 240
G+P PLRFSCNSWL+PKS S D RIFFTTKSYLPSNTP+GLKR R E+LK LQGNG+GK
Sbjct: 181 GIPTAPLRFSCNSWLTPKSQSDDTRIFFTTKSYLPSNTPKGLKRIRAEELKKLQGNGYGK 240
Query: 241 RESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSN 300
RE+HHRIYDYDVYNDLGDPD+G EF+RP+LGGKQHPYPRRCRTGRAR Q D LSEV S +
Sbjct: 241 RETHHRIYDYDVYNDLGDPDKGVEFQRPVLGGKQHPYPRRCRTGRARCQTDSLSEVRSWD 300
Query: 301 FYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSS-KYTW 360
Y+PRDEAF + V T YS+L+ LE D + T + K W
Sbjct: 301 IYLPRDEAFSRIEYVRLLG-NTFYSLLNSL--KLETTITDTKFSITEELSNSNKGIKGIW 360
Query: 361 EPSALLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVT 420
+A+LP+L V K V IL L PS D DKFFWF+DEEFARQTLAGLNP SIRLV
Sbjct: 361 NFNAILPKLFSSVVTKAVDILTL-PSSETFDKDKFFWFRDEEFARQTLAGLNPYSIRLVK 420
Query: 421 EWPLESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQ 480
EWPL+S+L+P IYGP +SAIT+EIVE+QIKG MTL
Sbjct: 421 EWPLKSQLEPDIYGPPESAITDEIVERQIKGVMTL------------------------- 480
Query: 481 NIPLLTVDCPWFSLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRS 540
Sbjct: 481 ------------------------------------------------------------ 540
Query: 541 PSLLRSRSRMKEDEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTL 600
+EAV+QKKLYILDYHDL +PYVAKVRK++GKTLYGSRTLFFLNPD TL
Sbjct: 541 ------------EEAVKQKKLYILDYHDLLVPYVAKVRKLKGKTLYGSRTLFFLNPDGTL 600
Query: 601 KPLAIELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHC 660
+PLAIEL+RPP+DGK QWKDV TPC ++TGLWLW+I KAHVLAHDSG+HQLVSHWLRTHC
Sbjct: 601 RPLAIELSRPPMDGKAQWKDVLTPCWDATGLWLWKIGKAHVLAHDSGHHQLVSHWLRTHC 660
Query: 661 SVEPYIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSME 720
VEPYIIA HRQLSAMHPIYRLLHPHFRYTME NAIAR +L N GGIIET FS GKYSME
Sbjct: 661 CVEPYIIATHRQLSAMHPIYRLLHPHFRYTMETNAIARSSLINEGGIIETTFSPGKYSME 720
Query: 721 VSSMVYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEW 780
SS+ Y++ WQFNLEALPADLIHRG+AVEDPNA HG+KLSIEDYP+ANDGLILWDAIKEW
Sbjct: 721 FSSLAYRQHWQFNLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPYANDGLILWDAIKEW 780
Query: 781 ATEYVNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMW 840
A EYVNHYYPDP VVKSD+ELQAWWTEIR VGHGDKKEEPWWP+L+TP+DLID+V+++MW
Sbjct: 781 AMEYVNHYYPDPSVVKSDEELQAWWTEIREVGHGDKKEEPWWPVLDTPKDLIDIVTTIMW 840
Query: 841 VTSGHHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHI 900
+T+GHHA NFGQY FA YFP RPS+AR NVPTE+P K WK FLE PE VFL+TFP I
Sbjct: 841 ITTGHHAVANFGQYSFAKYFPNRPSIARANVPTEQP-KKHWKYFLEKPENVFLETFPTQI 875
Query: 901 QATVLLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNAN 960
QAT++ S+ S+L++H P+E+YLG+DME AW D+P + QAF+KFS +L+KLE IID+RN N
Sbjct: 901 QATIVSSVASVLASHPPDEKYLGKDMEAAWADEPNINQAFNKFSEKLRKLEKIIDERNVN 875
Query: 961 PKLKNRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 996
P+LKNRHGAG++PY+LLKP+SEPGVT +GVP S+SI
Sbjct: 961 PELKNRHGAGIEPYELLKPHSEPGVTGKGVPFSISI 875
BLAST of Moc02g01030 vs. ExPASy TrEMBL
Match:
A0A6J1H3S4 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459733 PE=3 SV=1)
HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 610/984 (61.99%), Postives = 713/984 (72.46%), Query Frame = 0
Query: 15 FVYRNRNPFVENNVEALL-LKHPI--PNTPRLRGRFASASPSTAVKASVAAPMENPFSAH 74
F+Y + P + NV+A + L+H + P LR + +AV SV + ++
Sbjct: 18 FLY--QKPLLPGNVDACVSLQHSLTPPAASPLRFTSPNVIKPSAVHVSVPSIQSPVYTKA 77
Query: 75 VVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLEKGTIKGYA 134
VV TV+R + + L P G L+D+ + ++ L ELLS +MDPTTGLEKG +K Y
Sbjct: 78 VVTTVQRPIHQ-LFPKFGFIGRLHDIINR--RSRVLALELLSADMDPTTGLEKGRMKAYE 137
Query: 135 QKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVPYGPLRFSC 194
QKV R ED EI YE V IPA+FG IGAVLVEN + E+FLKDIVIHG+P GPL FSC
Sbjct: 138 QKVKR--EDDEIIYEISFV-IPADFGPIGAVLVENMHKKEVFLKDIVIHGIPTGPLHFSC 197
Query: 195 NSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRESHHRIYDYD 254
NSW++ K+ +DRRIFFTTKSYLPS TP+GLKR R E+L+ILQGNG+GKRE RIYDYD
Sbjct: 198 NSWITSKAQCNDRRIFFTTKSYLPSKTPDGLKRLRLEELRILQGNGYGKREKQERIYDYD 257
Query: 255 VYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYVPRDEAFID 314
VYNDLG+PD+GDE+KRP+LGGKQHPYPRRCRTGR R+Q DP SEV S FYVPRDEAF
Sbjct: 258 VYNDLGNPDKGDEYKRPVLGGKQHPYPRRCRTGRPRTQTDPSSEVKSLEFYVPRDEAFSI 317
Query: 315 TKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSALLPRLIDF 374
P K ++SML +PAL+ I D + SK+TW ++LLP L+
Sbjct: 318 PMIQENPLTKALHSMLSALLPALDTIGTDKQCSL---------SKHTWRFNSLLPTLMG- 377
Query: 375 AVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAI 434
ILLL SQ + DKFFWFKDEEFARQTLAGLNP SIRLVTEWPLESKL+P+I
Sbjct: 378 -------ILLLDSSQTSNNGDKFFWFKDEEFARQTLAGLNPYSIRLVTEWPLESKLEPSI 437
Query: 435 YGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWF 494
YG +SAIT+EIVEQQIKGFMT+
Sbjct: 438 YGSPESAITDEIVEQQIKGFMTI------------------------------------- 497
Query: 495 SLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKE 554
Sbjct: 498 ------------------------------------------------------------ 557
Query: 555 DEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPI 614
DEAV+Q+KL+ILDYHDLF+PYVAKVR+++G+TLYGSRTLFFLNPDNTL+PLAIEL+RPPI
Sbjct: 558 DEAVKQRKLFILDYHDLFIPYVAKVRELKGRTLYGSRTLFFLNPDNTLRPLAIELSRPPI 617
Query: 615 DGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQ 674
D K QWKDVFTPC ++ GLWLWRIAKAHVLAHDSG+HQLVSHWLRTHC VEPY+IA HRQ
Sbjct: 618 DDKPQWKDVFTPCWDAYGLWLWRIAKAHVLAHDSGHHQLVSHWLRTHCCVEPYVIATHRQ 677
Query: 675 LSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQF 734
LSAMHPIYRLLHPHFR+TM INA+ARE L NV G++E+ FS GKYSME+SS+VY+KQWQF
Sbjct: 678 LSAMHPIYRLLHPHFRHTMAINAVARETLINVEGLVESIFSPGKYSMEISSVVYEKQWQF 737
Query: 735 NLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDP 794
NLEALPADLIHRG+AVEDPNA HG+KLSIEDYPFANDGLILWDAIK+W TEYVNHYYPDP
Sbjct: 738 NLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPFANDGLILWDAIKQWVTEYVNHYYPDP 797
Query: 795 GVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFG 854
+V SD ELQAWWTEIR VGHGDKK+EPWWPILNTP DLI +V++++WVTSGHHAAVNFG
Sbjct: 798 SLVNSDGELQAWWTEIREVGHGDKKDEPWWPILNTPNDLIHIVTTIIWVTSGHHAAVNFG 857
Query: 855 QYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSIL 914
QY F SY+P RPS+ R+N+PTEEPNS L K FLE+PE VFLDTFP IQA +LL IL+IL
Sbjct: 858 QYPFTSYYPIRPSLTRLNIPTEEPNSTLRKYFLENPENVFLDTFPTQIQANILLFILNIL 879
Query: 915 STHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVD 974
S+HS +EEYLG DMEPAWGD+PV+K+AF+KFS +LKKLE+IID+RN NPKLKNRHGAGV
Sbjct: 918 SSHSRDEEYLGADMEPAWGDEPVIKEAFEKFSRKLKKLEEIIDERNGNPKLKNRHGAGVG 879
Query: 975 PYQLLKPYSEPGVTARGVPCSVSI 996
PYQ+LKPYSEPGVTARGVPCSVSI
Sbjct: 978 PYQILKPYSEPGVTARGVPCSVSI 879
BLAST of Moc02g01030 vs. ExPASy TrEMBL
Match:
A0A6J1K0X9 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491398 PE=3 SV=1)
HSP 1 Score: 1203.7 bits (3113), Expect = 0.0e+00
Identity = 605/984 (61.48%), Postives = 713/984 (72.46%), Query Frame = 0
Query: 15 FVYRNRNPFVENNV-EALLLKHPIPNTPRLRGRFASAS--PSTAVKASVAAPMENPFSAH 74
F+Y + P++ N E + L+H + T RF S + +AV V + ++
Sbjct: 18 FLY--QKPWLPGNADECVSLQHSLTPTAASPLRFTSPNVIKPSAVHVPVPSIQTPVYTKA 77
Query: 75 VVVTVKRHVGRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLEKGTIKGYA 134
VV TV+R + + L P G L+D+ D +K L ELLS +MDPTTGLEKG +K Y
Sbjct: 78 VVTTVQRPIHQ-LFPKFGFIGRLHDIIDR--RSKVLDLELLSADMDPTTGLEKGRMKAYE 137
Query: 135 QKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVPYGPLRFSC 194
+KV R ED EI YE V IPA+FG IGAVLVE+ + ++FLKDIVIHG+P GPL FSC
Sbjct: 138 EKVKR--EDDEIIYEISFV-IPADFGPIGAVLVESMHKKDVFLKDIVIHGIPTGPLHFSC 197
Query: 195 NSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRESHHRIYDYD 254
NSW++ K+ +DRRIFFTTKSYLPS TP+GLKR R ++L+ LQGNG+GKR+ H RIYDYD
Sbjct: 198 NSWITSKAQCNDRRIFFTTKSYLPSKTPDGLKRLRLQELRNLQGNGYGKRDKHQRIYDYD 257
Query: 255 VYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYVPRDEAFID 314
VYND+G+PD+G+E+KRP+LGGKQHPYPRRCRTGRAR+Q DP SEV S FYVPRDEAF
Sbjct: 258 VYNDIGNPDKGEEYKRPVLGGKQHPYPRRCRTGRARTQTDPSSEVKSPKFYVPRDEAFSI 317
Query: 315 TKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSALLPRLIDF 374
P K ++SML +PAL+ I+ + SK+TW+ ++ LP
Sbjct: 318 PMIQENPLTKALHSMLSALLPALDTISTHKQCNL---------SKHTWKFNSFLP----- 377
Query: 375 AVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAI 434
+ ILLL SQ + DKFFWFKDEEFARQTLAGLNP SIRLVTEWPL+SKL+P+I
Sbjct: 378 ---TPMGILLLDSSQISNNGDKFFWFKDEEFARQTLAGLNPYSIRLVTEWPLKSKLEPSI 437
Query: 435 YGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWF 494
YG +SAIT+EIVEQQIKGFMTL
Sbjct: 438 YGSPESAITDEIVEQQIKGFMTL------------------------------------- 497
Query: 495 SLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKE 554
Sbjct: 498 ------------------------------------------------------------ 557
Query: 555 DEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPI 614
DEAV+Q+KL+ILDYHDLF+PYVAKVR+++G+TLYGSRTLFFLNPDNTL+PLAIEL+RPPI
Sbjct: 558 DEAVKQRKLFILDYHDLFIPYVAKVRELKGRTLYGSRTLFFLNPDNTLRPLAIELSRPPI 617
Query: 615 DGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQ 674
D K QWKDVFTPC ++ GLWLWRIAKAHVLAHDSGYHQLVSHWLRTHC VEPY+IA HRQ
Sbjct: 618 DDKPQWKDVFTPCWDAYGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCCVEPYVIATHRQ 677
Query: 675 LSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQF 734
LSAMHPIYRLLHPHFR+TM INA+ARE L NV G+IE+ FS GKYSME+SS+VY+KQWQF
Sbjct: 678 LSAMHPIYRLLHPHFRHTMAINAVARETLINVEGLIESIFSPGKYSMEISSVVYEKQWQF 737
Query: 735 NLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDP 794
NLEALPADLIHRG+AVEDPNA HG+KLSIEDYPFANDGLILWDAIK+W TEYVNHYYPDP
Sbjct: 738 NLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPFANDGLILWDAIKQWVTEYVNHYYPDP 797
Query: 795 GVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFG 854
+V SD ELQAWWTEIR VGHGDKK+EPWWPILNTP DLI +V++++WVTSGHHAAVNFG
Sbjct: 798 SLVNSDGELQAWWTEIREVGHGDKKDEPWWPILNTPNDLIHIVTTIIWVTSGHHAAVNFG 857
Query: 855 QYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSIL 914
QY F SY+P RPS+ R+N+PTEEPNS LWK FLE+PE VFLDTFP IQA +LL IL+IL
Sbjct: 858 QYPFTSYYPIRPSLTRLNIPTEEPNSTLWKYFLENPENVFLDTFPTQIQANILLFILNIL 879
Query: 915 STHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVD 974
S+HS +EEYLG DMEPAWGD+PV+K+AF+KFS +LKKLE+IID+RN NPKLKNRHGAGV
Sbjct: 918 SSHSRDEEYLGADMEPAWGDEPVIKEAFEKFSRKLKKLEEIIDERNGNPKLKNRHGAGVG 879
Query: 975 PYQLLKPYSEPGVTARGVPCSVSI 996
PYQ+LKPYSEPGVTARGVPCSVSI
Sbjct: 978 PYQILKPYSEPGVTARGVPCSVSI 879
BLAST of Moc02g01030 vs. TAIR 10
Match:
AT3G45140.1 (lipoxygenase 2 )
HSP 1 Score: 867.1 bits (2239), Expect = 1.4e-251
Identity = 446/925 (48.22%), Postives = 584/925 (63.14%), Query Frame = 0
Query: 85 LPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLEKGTIKGYAQKVGREEEDGEIT 144
L + R L D+ D ++L+ EL+S + D ++ T++ YAQ+V E D +
Sbjct: 86 LEGITWSRGLDDIADI--RGRSLLVELISAKTD-----QRITVEDYAQRVWAEAPDEK-- 145
Query: 145 YETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVPYGPLRFSCNSWLSPKSHSHDR 204
YE E +P +FG +GA+ ++NQY+ +LFLK + + +P G + F+C SW++PKS +
Sbjct: 146 YECEF-EMPEDFGPVGAIKIQNQYHRQLFLKGVELK-LPGGSITFTCESWVAPKSVDPTK 205
Query: 205 RIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGH---GKRESHHRIYDYDVYNDLGDPDR 264
RIFF+ KSYLPS TPE LK++R+E+L+ LQG G+ RIYDYDVYND+GDPD
Sbjct: 206 RIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTKFERIYDYDVYNDVGDPDN 265
Query: 265 GDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVT-SSNFYVPRDEAFIDTKQVTTPAV 324
E RP++GG HPYPRRC+TGR + DP SE FYVPRDE F T + T+
Sbjct: 266 DPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEF-STAKGTSFTG 325
Query: 325 KTVYSMLHGFIPALEAIA----------QDIESRFTNRVMLLPSSKYTWEPSALLPRLID 384
K V + L P +E++ + I++ F + L + LLPR+I
Sbjct: 326 KAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKDAGLL----PLLPRIIK 385
Query: 385 FAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPA 444
A+ + +L + +++ D+F W +D+EFARQTLAGLNP SI+LV EWPL SKLDPA
Sbjct: 386 -ALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPA 445
Query: 445 IYGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPW 504
+YG S IT EIVE+++KG MT+
Sbjct: 446 VYGDPTSLITWEIVEREVKGNMTV------------------------------------ 505
Query: 505 FSLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMK 564
Sbjct: 506 ------------------------------------------------------------ 565
Query: 565 EDEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPP 624
DEA++ K+L++LDYHDL LPYV KVR++ TLY SRTLFFL+ D+TL+P+AIELT PP
Sbjct: 566 -DEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPP 625
Query: 625 IDGKQQWKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHR 684
K QWK VFTP ++T WLW +AK H ++HD+GYHQL+SHWLRTH EPYIIA +R
Sbjct: 626 NINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANR 685
Query: 685 QLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQ 744
QLSAMHPIYRLLHPHFRYTMEINA AR++L N GGIIET F GKY++E+SS VY K W+
Sbjct: 686 QLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWR 745
Query: 745 FNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPD 804
F+ E LPADLI RG+A ED A HGV+L+I DYPFANDGLILWDAIKEW T+YV HYYPD
Sbjct: 746 FDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPD 805
Query: 805 PGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNF 864
++ SD+ELQ WW+E+R++GHGDKK+EPWWP+L T +DLI VV+++ WVTSGHHAAVNF
Sbjct: 806 EELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNF 865
Query: 865 GQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSI 924
GQY + YFP RP+ RI +PTE+P + K F E PE+V L T+P+ QAT+++ L +
Sbjct: 866 GQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDL 896
Query: 925 LSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGV 984
LSTHSP+EEY+GE E +W ++PV+ AF++F G+L+ LE +ID+RN N LKNR GAGV
Sbjct: 926 LSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGV 896
Query: 985 DPYQLLKPYSEPGVTARGVPCSVSI 996
Y+LLKP SE GVT GVP S+SI
Sbjct: 986 VKYELLKPTSEHGVTGMGVPYSISI 896
BLAST of Moc02g01030 vs. TAIR 10
Match:
AT1G17420.1 (lipoxygenase 3 )
HSP 1 Score: 707.2 bits (1824), Expect = 1.8e-203
Identity = 366/907 (40.35%), Postives = 537/907 (59.21%), Query Frame = 0
Query: 105 KTLVFELLSTEMDPTTGLEK----GTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIG 164
+ +V EL+ST++DP T L K +K +++K + E + T + A FG+ G
Sbjct: 117 RNIVLELISTQLDPKTKLPKKSNAAVLKDWSKK---SKTKAERVHYTAEFTVDAAFGSPG 176
Query: 165 AVLVENQYNTELFLKDIVIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPE 224
A+ V N++ E FL+ I I G GP+ F CNSW+ + D+RIFFT + YLP+ TP
Sbjct: 177 AITVMNKHQKEFFLESITIEGFALGPVHFPCNSWVQSQKDHPDKRIFFTNQPYLPNETPS 236
Query: 225 GLKRFREEDLKILQGNGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRR 284
GL+ RE++LK L+G+G G R+ RIYD+DVYNDLG+PD+ E RP LGGK+ PYPRR
Sbjct: 237 GLRVLREKELKNLRGDGSGVRKLSDRIYDFDVYNDLGNPDKSSELSRPKLGGKEVPYPRR 296
Query: 285 CRTGRARSQADPLSEVTSSN---FYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEA- 344
CRTGR + +D +E YVPRDE F ++KQ T A + + ++LH IP+L+A
Sbjct: 297 CRTGRQSTVSDKDAESRVEKPLPMYVPRDEQFEESKQDTFAAGR-LKAVLHHLIPSLKAS 356
Query: 345 -IAQD------IESRFTNRVMLLPSSKYTWEPSALLPRLIDFAVDKVVRILLLGPSQAVL 404
+A+D I+ + ++L + LP+++ + + + LL + +L
Sbjct: 357 IVAEDFADFGEIDRLYKEGLLLKLGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTPKIL 416
Query: 405 DSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAIYGPTKSAITNEIVEQQIK 464
DK W +D+EFARQ +AG+NP +I V +P S LDP IYGP SA+T++ I
Sbjct: 417 SKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDD----HII 476
Query: 465 GFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWFSLFLSLLFLSHLRPAATV 524
G + DGF V
Sbjct: 477 GHL-------DGFSV--------------------------------------------- 536
Query: 525 DQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKEDEAVEQKKLYILDYHDLF 584
+A+E+ +LY+LDYHD+F
Sbjct: 537 ------------------------------------------QQALEENRLYMLDYHDIF 596
Query: 585 LPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQWKDVFTPCLNSTG 644
LP++ ++ ++G+ Y +RT+FFL TLKP+AIEL+ PP K + K V TP +++T
Sbjct: 597 LPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIELSLPPHGPKHRSKRVLTPPVDATS 656
Query: 645 LWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFRYT 704
W+W++AKAHV ++D+G HQLV+HWLRTH +EP+I+A HRQLSAMHPI++LL PH RYT
Sbjct: 657 NWMWQLAKAHVSSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYT 716
Query: 705 MEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEALPADLIHRGMAVED 764
+EINA+AR++L + G+IE F+ G Y ME+S+ Y+ W+F++E LPADLI RGMA+ D
Sbjct: 717 LEINALARQSLISADGVIEGGFTAGAYGMEMSAAAYKSSWRFDMEGLPADLIRRGMAIPD 776
Query: 765 PNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKSDKELQAWWTEIRS 824
HG+KL IEDYP+ANDGL+LW AI+ W YV YYP+P ++K+D ELQ+W++E +
Sbjct: 777 ATQPHGLKLLIEDYPYANDGLLLWSAIQTWVRTYVERYYPNPNLIKTDSELQSWYSESIN 836
Query: 825 VGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFASYFPARPSVARIN 884
VGH D ++ WWP L+T +DL+ ++++++W+ S HAA+NFGQY + Y P RP + R
Sbjct: 837 VGHADLRDADWWPELSTVDDLVSILTTLIWLASAQHAALNFGQYPYGGYVPNRPPLMRRL 896
Query: 885 VPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSPEEEYLGEDMEPA- 944
+P E + + F+ PE+ + + P+ Q + ++++ LSTHSP+EEY+GE +P+
Sbjct: 897 IPDE--SDPEYASFISHPEKYYFSSMPSLAQTSKFMAVVDTLSTHSPDEEYIGERQQPSI 919
Query: 945 WGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVDPYQLLKPYSEPGVTARG 996
W D + +AF F+ + ++E I++RNA+P +NR GAGV PY+LL P SEPGVT RG
Sbjct: 957 WTGDAEIVEAFYGFAAEIGRIEKEIEKRNADPDRRNRCGAGVLPYELLVPSSEPGVTCRG 919
BLAST of Moc02g01030 vs. TAIR 10
Match:
AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )
HSP 1 Score: 700.7 bits (1807), Expect = 1.7e-201
Identity = 364/909 (40.04%), Postives = 535/909 (58.86%), Query Frame = 0
Query: 105 KTLVFELLSTEMDPTTGLEK----GTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIG 164
+ +V EL+ST++DP T K +K +++K + E T E + + FG+ G
Sbjct: 123 RNVVLELMSTQVDPKTNEPKKSKAAVLKDWSKKSNSKAERVHYTAE---FTVDSAFGSPG 182
Query: 165 AVLVENQYNTELFLKDIVIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPE 224
A+ V N++ E FL+ I I G GP+ F CNSW+ + +RI FT + YLPS TP
Sbjct: 183 AITVTNKHQKEFFLESITIEGFACGPVHFPCNSWVQSQKDHPSKRILFTNQPYLPSETPS 242
Query: 225 GLKRFREEDLKILQGNGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRR 284
GL+ RE++L+ L+GNG G+R+ RIYDYDVYND+G+PD E RP LGG++ PYPRR
Sbjct: 243 GLRTLREKELENLRGNGKGERKLSDRIYDYDVYNDIGNPDISRELARPTLGGREFPYPRR 302
Query: 285 CRTGRARSQADPLSEVTSSN---FYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEA- 344
CRTGR+ + D +SE YVPRDE F ++KQ T A + + ++LH IP+L+A
Sbjct: 303 CRTGRSSTDTDMMSERRVEKPLPMYVPRDEQFEESKQNTFAACR-LKAVLHNLIPSLKAS 362
Query: 345 -IAQD------IESRFTNRVMLLPSSKYTWEPSALLPRLIDFAVDKVVRILLLGPSQAVL 404
+A+D I+S + ++L + LP+++ + K LL + ++
Sbjct: 363 ILAEDFANFGEIDSLYKEGLLLKLGFQDDMFKKFPLPKIVT-TLQKSSEGLLRYDTPKIV 422
Query: 405 DSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAIYGP-TKSAITNEIVEQQI 464
DK+ W +D+EFARQ +AG+NP +I VT +P S LDP IYGP SA+T + + Q+
Sbjct: 423 SKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHIIGQL 482
Query: 465 KGFMTLNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWFSLFLSLLFLSHLRPAAT 524
G LTV
Sbjct: 483 DG---------------------------------LTV---------------------- 542
Query: 525 VDQPSAVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKEDEAVEQKKLYILDYHDL 584
+A+E +L+++DYHD+
Sbjct: 543 -------------------------------------------QQALETNRLFMVDYHDI 602
Query: 585 FLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRP-PIDGKQQWKDVFTPCLNS 644
+LP++ ++ ++G+ Y +RT+ FL TLKP+AIEL+ P Q+ K V TP +++
Sbjct: 603 YLPFLDRINALDGRKAYATRTILFLTRLGTLKPIAIELSLPSQSSSNQKSKRVVTPPVDA 662
Query: 645 TGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFR 704
T W+W++AKAHV ++D+G HQLV+HWLRTH +EP+I+A HRQLSAMHPI++LL PH R
Sbjct: 663 TSNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMR 722
Query: 705 YTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEALPADLIHRGMAV 764
YT+EINA+AR+ L + G+IE+ F+ G+Y +E+SS Y+ +W+F++E LPADLI RGMAV
Sbjct: 723 YTLEINAVARQTLISADGVIESCFTAGQYGLEISSAAYKNKWRFDMEGLPADLIRRGMAV 782
Query: 765 EDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKSDKELQAWWTEI 824
DP HG+KL +EDYP+ANDGL+LW AI+ W YV YY + ++++D ELQAW++E
Sbjct: 783 PDPTQPHGLKLLVEDYPYANDGLLLWSAIQTWVRTYVERYYANSNLIQTDTELQAWYSES 842
Query: 825 RSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFASYFPARPSVAR 884
+VGH D ++ WWP L+T EDL+ V+++++W+ S HAA+NFGQY + Y P RP + R
Sbjct: 843 INVGHADHRDAEWWPKLSTVEDLVSVITTIIWLASAQHAALNFGQYPYGGYVPNRPPLMR 902
Query: 885 INVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSPEEEYLGEDMEP 944
+P E + + F+EDP++ F + P+ +Q T ++++ LSTHSP+EEY+GE +P
Sbjct: 903 RLIPDE--SDPEFTSFIEDPQKYFFSSMPSLLQTTKFMAVVDTLSTHSPDEEYIGERQQP 926
Query: 945 A-WGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVDPYQLLKPYSEPGVTA 996
+ W D + AF FS + ++E ID+RN +P +NR GAGV PY+L+ P SEPGVT
Sbjct: 963 SIWTGDAEIVDAFYGFSAEIGRIEKEIDKRNRDPSRRNRCGAGVLPYELMAPSSEPGVTC 926
BLAST of Moc02g01030 vs. TAIR 10
Match:
AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )
HSP 1 Score: 683.3 bits (1762), Expect = 2.9e-196
Identity = 363/907 (40.02%), Postives = 529/907 (58.32%), Query Frame = 0
Query: 107 LVFELLSTEMDPTTGLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVEN 166
++ +L+S E+DP TG + +++ + + +D T +P FG GA+LV N
Sbjct: 115 MLIQLVSEEIDPETGKGRKSLESPVMGLPKAVKDPRYLVFTADFTVPINFGKPGAILVTN 174
Query: 167 QYNTELFLKDIVIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFR 226
+TE+ L +I+I + F N+W+ K+ + RI F ++ LPS TP+G+K R
Sbjct: 175 LLSTEICLSEIIIED-STDTILFPANTWIHSKNDNPQARIIFRSQPCLPSETPDGIKELR 234
Query: 227 EEDLKILQGNGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRA 286
E+DL ++G+G G+R+ H RIYDYDVYNDLGDP R E RP+LG + PYPRRCRTGR
Sbjct: 235 EKDLVSVRGDGKGERKPHERIYDYDVYNDLGDP-RKTERVRPVLGVPETPYPRRCRTGRP 294
Query: 287 RSQADPLSE---VTSSNFYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIA----- 346
DP E FYVPRDE F + K+ T A + ++ H +P++ A
Sbjct: 295 LVSKDPPCESRGKEKEEFYVPRDEVFEEIKRDTFRAGR-FKALFHNLVPSIAAALSNLDI 354
Query: 347 -----QDIESRFTNRVMLLPSSKYTWEPSALLPRLIDFAVDKVVRILLLGPSQAVLDSDK 406
DI++ + + ++L + + ++ + V LL + AV+ D+
Sbjct: 355 PFTCFSDIDNLYKSNIVLGHTEPKDTGLGGFIGGFMN-GILNVTETLLKYDTPAVIKWDR 414
Query: 407 FFWFKDEEFARQTLAGLNPCSIRLVTEWPLESKLDPAIYGPTKSAITNEIVEQQIKGFMT 466
F W +D EF RQ LAG+NP +I L+ E P+ S LDPA+YGP +S +T EI+ ++++ + T
Sbjct: 415 FAWLRDNEFGRQALAGVNPVNIELLKELPIRSNLDPALYGPQESVLTEEIIAREVEHYGT 474
Query: 467 LNECRFDGFGVGVYLIPKFQNGIFGQNIPLLTVDCPWFSLFLSLLFLSHLRPAATVDQPS 526
E
Sbjct: 475 TIE--------------------------------------------------------- 534
Query: 527 AVAALSPLHYHRRALSFAIVGKRMRSPSLLRSRSRMKEDEAVEQKKLYILDYHDLFLPYV 586
+A+E+K+L+++DYHD+ LP+V
Sbjct: 535 ---------------------------------------KALEEKRLFLVDYHDILLPFV 594
Query: 587 AKVRKI--EGKTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQWKDVFTPCLNSTGLW 646
K+ I + + Y SRT+FF + + L+PLAIEL+ PP + + K V+T ++T W
Sbjct: 595 EKINSIKEDPRKTYASRTIFFYSKNGALRPLAIELSLPP-TAESENKFVYTHGHDATTHW 654
Query: 647 LWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFRYTME 706
+W++AKAHV ++D+G HQLV+HWLRTH S+EPYIIA +RQLS MHP+Y+LLHPH RYT+E
Sbjct: 655 IWKLAKAHVCSNDAGVHQLVNHWLRTHASMEPYIIATNRQLSTMHPVYKLLHPHMRYTLE 714
Query: 707 INAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEALPADLIHRGMAVEDPN 766
INA AR++L N GGIIE+ F+ GKY+ME+SS Y+ W+F++E LPADL+ RGMA ED +
Sbjct: 715 INARARKSLINGGGIIESCFTPGKYAMELSSAAYKSMWRFDMEGLPADLVRRGMAEEDSS 774
Query: 767 AAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKSDKELQAWWTEIRSVG 826
A GV+L I+DYP+A DGL++W AIK+ YV H+Y D + SD ELQAWW EI++ G
Sbjct: 775 AECGVRLVIDDYPYAADGLLIWKAIKDLVESYVKHFYSDSKSITSDLELQAWWDEIKNKG 834
Query: 827 HGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFASYFPARPSVARINVP 886
H DKK+EPWWP LNT +DL ++++M+W+ SG HAA+NFGQY F Y P RP++ R +P
Sbjct: 835 HYDKKDEPWWPKLNTTQDLSQILTNMIWIASGQHAAINFGQYPFGGYVPNRPTLLRKLIP 894
Query: 887 TE-EPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSPEEEYLGE--DMEPA 946
E +P+ ++ F+ +P+ FL + P +QAT ++++ LSTHSP+EEYL E +++
Sbjct: 895 QETDPD---YEMFMRNPQYSFLGSLPTQLQATKVMAVQETLSTHSPDEEYLIELREVQRH 917
Query: 947 WGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVDPYQLLKPYSEPGVTARG 996
W D V + F+KFS L K+E I++RN + KLKNR GAG+ PY+LL P S GVT RG
Sbjct: 955 WFQDEQVVKYFNKFSEELVKIEKTINERNKDKKLKNRTGAGMPPYELLLPTSPHGVTGRG 917
BLAST of Moc02g01030 vs. TAIR 10
Match:
AT1G55020.1 (lipoxygenase 1 )
HSP 1 Score: 596.7 bits (1537), Expect = 3.5e-170
Identity = 347/881 (39.39%), Postives = 484/881 (54.94%), Query Frame = 0
Query: 142 EITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVP-YGPLRFSCNSWLSPKSH 201
++T++ E +FG GA L+ N + +E LK + + VP +G + + CNSW+ P H
Sbjct: 98 KVTFDYE-----TDFGYPGAFLIRNSHFSEFLLKSLTLEDVPGHGRVHYICNSWIYPAKH 157
Query: 202 SHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRESHHRIYDYDVYNDLGDPD 261
R+FF+ K+YLP TP L ++REE+L L+G G G+ + R+YDY YNDLG P
Sbjct: 158 YTTDRVFFSNKTYLPHETPATLLKYREEELVSLRGTGEGELKEWDRVYDYAYYNDLGVPP 217
Query: 262 RGDEFKRPILGGKQ-HPYPRRCRTGRARSQADPLSE----VTSS-NFYVPRDEAF--IDT 321
+ RP+LGG Q +PYPRR RTGR ++ DP +E +TSS + YVPRDE F +
Sbjct: 218 KN---PRPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRLPITSSLDIYVPRDERFGHLKM 277
Query: 322 KQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVMLLPSSKYTWEPSALLPR--LID 381
A+K + + PALEA+ D F + +L +E LP LID
Sbjct: 278 SDFLAYALKAIAQFIQ---PALEAVFDDTPKEFDSFEDVLK----IYEEGIDLPNQALID 337
Query: 382 FAVDKVV------------RILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLV 441
V + + L P V+ DK W DEEFAR+ LAGLNP I+L+
Sbjct: 338 SIVKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDKTAWRTDEEFAREMLAGLNPVVIQLL 397
Query: 442 TEWPLESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNECRFDGFGVGVYLIPKFQNGIFG 501
E+P +SKLD YG S IT +E + G
Sbjct: 398 KEFPPKSKLDSESYGNQNSTITKSHIEHNLDG---------------------------- 457
Query: 502 QNIPLLTVDCPWFSLFLSLLFLSHLRPAATVDQPSAVAALSPLHYHRRALSFAIVGKRMR 561
LTV
Sbjct: 458 -----LTV---------------------------------------------------- 517
Query: 562 SPSLLRSRSRMKEDEAVEQKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNT 621
+EA+E+++L+ILD+HD +PY+ +V KT Y SRTL FL D T
Sbjct: 518 -------------EEALEKERLFILDHHDTLMPYLGRVNTTTTKT-YASRTLLFLKDDGT 577
Query: 622 LKPLAIELTRPPIDGKQ--QWKDVFTPCLNSTGLW--LWRIAKAHVLAHDSGYHQLVSHW 681
LKPL IEL+ P +G + +V+TP G++ LW++AKA V +DSG HQL+SHW
Sbjct: 578 LKPLVIELSLPHPNGDKFGAVSEVYTP---GEGVYDSLWQLAKAFVGVNDSGNHQLISHW 637
Query: 682 LRTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLG 741
++TH S+EP++IA +RQLS +HP+++LL PHFR TM INA+AR+ L N GGI E
Sbjct: 638 MQTHASIEPFVIATNRQLSVLHPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPS 697
Query: 742 KYSMEVSSMVYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWD 801
KY+ME+SS +Y+ W F +ALPA+L RGMAVEDP A HG++L I+DYP+A DGL +W
Sbjct: 698 KYAMEMSSFIYKNHWTFPDQALPAELKKRGMAVEDPEAPHGLRLRIKDYPYAVDGLEVWY 757
Query: 802 AIKEWATEYVNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVV 861
AI+ W +Y+ +Y +++D ELQAWW E+R GHGDKK EPWWP + T E+L++
Sbjct: 758 AIESWVRDYIFLFYKIEEDIQTDTELQAWWKEVREEGHGDKKSEPWWPKMQTREELVESC 817
Query: 862 SSMMWVTSGHHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDT 921
+ ++WV S HAAVNFGQY A Y P RP+++R +P E N+ ++ ++P++VFL T
Sbjct: 818 TIIIWVASALHAAVNFGQYPVAGYLPNRPTISRQYMPKE--NTPEFEELEKNPDKVFLKT 859
Query: 922 FPAHIQATVLLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIID 981
A +Q + +S++ ILSTHS +E YLG+ W + +AF+KF ++K++E ID
Sbjct: 878 ITAQLQTLLGISLIEILSTHSSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNID 859
Query: 982 QRNANPKLKNRHGAGVDPYQLLKPYSEPGVTARGVPCSVSI 996
+RN + LKNR G PY LL P SE GVT RG+P SVSI
Sbjct: 938 ERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022148713.1 | 0.0e+00 | 89.59 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia] | [more] |
XP_022148734.1 | 0.0e+00 | 64.40 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia] | [more] |
XP_022958149.1 | 0.0e+00 | 61.65 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata] | [more] |
XP_022958530.1 | 0.0e+00 | 61.99 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata] | [more] |
XP_022996082.1 | 0.0e+00 | 61.48 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
O24370 | 1.2e-284 | 51.39 | Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO... | [more] |
R9WS04 | 1.0e-270 | 50.42 | Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=... | [more] |
P38418 | 2.0e-250 | 48.22 | Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1 | [more] |
Q8GSM2 | 5.3e-248 | 46.32 | Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1 | [more] |
Q84YK8 | 4.4e-242 | 46.83 | Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1D4V6 | 0.0e+00 | 89.59 | Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017309 PE=3 SV=1 | [more] |
A0A6J1D4V5 | 0.0e+00 | 64.40 | Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017327 PE=3 SV=1 | [more] |
A0A6J1H492 | 0.0e+00 | 61.65 | Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459464 PE=3 SV=1 | [more] |
A0A6J1H3S4 | 0.0e+00 | 61.99 | Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459733 PE=3 SV=1 | [more] |
A0A6J1K0X9 | 0.0e+00 | 61.48 | Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491398 PE=3 SV=1 | [more] |