Moc01g32950 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g32950
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description4a-hydroxytetrahydrobiopterin dehydratase
Locationchr1: 23150620 .. 23153589 (-)
RNA-Seq ExpressionMoc01g32950
SyntenyMoc01g32950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGGTAATTACCTTTCTCCGAATCCAACTGCACGTTTCCTTTATCATTTTATAGTACATGTCGAAGATCTCCAAGTCGAGAGGAAAACCGCTCCTGTGAATGAAAAAGTTGGGGTAAAATGTATCGCACGATTGGTGTTTGTATATATGTCTGATTCGCCCCCGTTTTGGTGTTTGCATGATGCATCTGTAATTGAGCAATCGCGGTTGAGAAACGCAGATTTGAAATAAGGTGGATCGCGATTTTGTTAATATCAGATAGAAATTAAGATTATCAGTCCAAATATCTTTGTAGTTCATCTATGCGCTCACATCCGTGTAGTGCAAGACAATCGAAGATAGATCCAAAAATTACGGTTGAAATGATAATTATGGTGTTCTGATGGTCCAAGCGTTAAAAACTTGAGTCCCGATCATTTTTGGTGGATTTCATATTGTCCATGACCAAAGTTGCTTTCAAATTATCATATTAGAAAACTTATTTTATTTAATCTGCGTCGGGCGCTATAGTTTTCCGGATACTGCTCTGATGTTGTTTATGCCAATGATCTCGCGTGTGAAGCCATTACGTTACAACCTTCTCTACCAGTGTATTTGTTTGTTCTATGATCTGCCGCATTTATGTGTGTTGTTTCATCAGTAAACAAATAATGCTTACTGCGATGCTCGTGTCAATAGCAATACAATATTAAAGTTTCATTTGTATGGGATTCCACGTTTTGTTTTACATTCAGACAGAGGTAATTTGTGTATGTACAGTCCAAATTCTCGAATTACTGGGATATGTTGGGAGCATGTGAGCTTACCATCCGGTAGAAAAACACTTTCTGGATTTAGAATGCTTTGTACTGGCGAAGGTGCGTGTTGGCCTTTAAAGCACTTTAAAATTTGTTAAGCAGCTATTACAGAGTGTCAACCTTATCTTTTATTTATTTACGAGCAAGGTTCTCTAGTTGATGGCTACTCCGACCAGAGCACTATCTTTTAATGTCAAGGAAACCTGTAGGGTCTCTTCGTATAGCGGCCATCTCGGCTCCTCCCAGTCTCCTTTCTAAAACGTGCGGCTTGTTTTGTCCCTTTGATTAAGAAAAATGTTTTTCACCTCCACTCTCTGGACTTGAAAAGCTCTGCATTGCATATAATTTCATGAGATCATATTAATCTCCTAGTCTCTGCTTGACATTTATTGAAGTCTATTGATCATTTGGGATCAATAAGTGTACTCTGACTGATGGTTCTATTTAGCCTACTAAATTATTTAGCAAGTTTTGGGACTATTTTTTGGGGAAATGAAATCTGTAGATGACCTTCCGGTCTTTTCTTAGGTCTCTATGTCAACCTTGGTTGATATTGGCTATTATTGCATTATGACCAGAACTGGGTGGGTGTTGTCTAATTGAGTTGAAGCATCATATCCGCATGTGTGTCAGTCGAAGTTGCAATTAGCAATGAATTATCACATCACGTGGTGCTGGTTTATTCTCTTTTTATGGTGCAGTCATATTGCTCTTGTGACATAATTGACCATTTTGTACATGCCAGATTTGTCAGCTAAAAAATGTGTGCCTTGCAACTCAAATGACTTGCGGCCAATGACCGAGGATGCAGCTAAACATCTGATTCAAGAGGTATGTGATATCTGTCTTTGTTTGTTAAAAACTATCATATTATACATTTCATTCATTTTTGTGGCCACTGTCTTATTGTAAAGCTAGCTGAGTGGCACCTGATAAACGAAGATGGTAAACTAAAGTTGAACCGATCATTGAAGGTGAAGAGTTTTCTTAAAGGAATGGAGTTATTTAAACTTATAGCTGAAGTTGCAGAAGCAGAGGGTATCTTGCTTTCCATCTACCAAATATACAATTCACACGTTTCTTTTTCCATGGATCACGATTAATGTAGTTGCAATATGAATTTTCAGAGAGGTCTAAATCTGATACTATAATTCAATGCAGGCCATCATCCTGATCTTCATCTAGTTGGATGGAACAATGTAACAATTGAGATCTGGACACATGCAGTAGGTAAGAATAATATTCTCTTCTATGAGGCATTCAATAAATTTTCGCAAACATTGAACTTAGACTTATTTGTTTCACTCATAATTTTAGTGTCCTATTTTAATTTGCGTAAAAGGCCATTTGAATGTGAATTTGGACCCGTCTTTCTTTGATTTTATTTCTAATTCTTGTACCTTGAAACAATATTCTTTACACTGTACGAATCTTCTGTAATATGGGATTTGAGCTTTGAAAATAATGTGGTAATGTAGCAATGAATTGGGGAACACCTTTATAATTGTTCTTTCGCTAACAATGAGCTGCCAAAACATCTAAGTCATATTGAAGTAGAAGAATGAAGTTTATAATTTTCTGCTGTTGTGTAAGCTTTTAGTGTCAATCTTCAATGAACGGTAAGGTAAGTTTTCAGGTAGCGTGTTCATTCAGATCTCAGGCTACGTTTTGGGCATGGCCGAAAGCACTTCAAACAAAACTGTATTGGAGTGGTTTTTGCCCTTTGGCGCCCAAGTACTCGCTAGAAAAACACTTCAAAGTACATAGAATTAAACATTAAAATGATATTAAGAATGTTAAACTAAAAGCAAAGTCTGGTTTGAGGAAATGCTCTCAAATGGCGATGGCTCAGAATTAGCTAGTTTTTCGTCTTCCACTCGACTGTATCTTCATCTAGCTTGTTTCTTGATTGGTTCCTTGCATCAATTAAAAGTAGTATCCTGGCCTTTTCTTCCCCGAGTTTTTTTGCTCTCCATTCTTACTGAAATCTATGTAATATATGGCTCTATTTCATAGCCCCCATTAAATGGAAGATGGTTCTGACACAATGAACTTTTGGGCAGGTGGGCTGACAGAAAATGACTTCATACTCGCTTCCAAGATCGATAAGCTTGACGTTCATAACCTACTCAGCCGGAAAGTTTCTATTAGCAGTCAACATGACTGCTGA

mRNA sequence

ATGGACGATTTGTCAGCTAAAAAATGTGTGCCTTGCAACTCAAATGACTTGCGGCCAATGACCGAGGATGCAGCTAAACATCTGATTCAAGAGCTAGCTGAGTGGCACCTGATAAACGAAGATGGTAAACTAAAGTTGAACCGATCATTGAAGGTGAAGAGTTTTCTTAAAGGAATGGAGTTATTTAAACTTATAGCTGAAGTTGCAGAAGCAGAGGGCCATCATCCTGATCTTCATCTAGTTGGATGGAACAATGTAACAATTGAGATCTGGACACATGCAGTAGGTGGGCTGACAGAAAATGACTTCATACTCGCTTCCAAGATCGATAAGCTTGACGTTCATAACCTACTCAGCCGGAAAGTTTCTATTAGCAGTCAACATGACTGCTGA

Coding sequence (CDS)

ATGGACGATTTGTCAGCTAAAAAATGTGTGCCTTGCAACTCAAATGACTTGCGGCCAATGACCGAGGATGCAGCTAAACATCTGATTCAAGAGCTAGCTGAGTGGCACCTGATAAACGAAGATGGTAAACTAAAGTTGAACCGATCATTGAAGGTGAAGAGTTTTCTTAAAGGAATGGAGTTATTTAAACTTATAGCTGAAGTTGCAGAAGCAGAGGGCCATCATCCTGATCTTCATCTAGTTGGATGGAACAATGTAACAATTGAGATCTGGACACATGCAGTAGGTGGGCTGACAGAAAATGACTTCATACTCGCTTCCAAGATCGATAAGCTTGACGTTCATAACCTACTCAGCCGGAAAGTTTCTATTAGCAGTCAACATGACTGCTGA

Protein sequence

MDDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMELFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRKVSISSQHDC
Homology
[Close]
BLAST of Moc01g32950 vs. NCBI nr
Match: XP_022142040.1 (uncharacterized protein LOC111012265 [Momordica charantia])

HSP 1 Score: 265.0 bits (676), Expect = 3.4e-67
Identity = 128/129 (99.22%), Postives = 129/129 (100.00%), Query Frame = 0 

Query: 2   DDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL 61
           +DLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL
Sbjct: 64  EDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL 123

Query: 62  FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 121
           FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK
Sbjct: 124 FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 183

Query: 122 VSISSQHDC 131
           VSISSQHDC
Sbjct: 184 VSISSQHDC 192

[Close]
BLAST of Moc01g32950 vs. NCBI nr
Match: XP_022925639.1 (uncharacterized protein LOC111432994 isoform X3 [Cucurbita moschata] >XP_023543300.1 uncharacterized protein LOC111803220 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 253.1 bits (645), Expect = 1.3e-63
Identity = 121/130 (93.08%), Postives = 126/130 (96.92%), Query Frame = 0 

Query: 1   MDDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGME 60
           MDDLS KKCVPCNS DLRPMTEDAAKHLI+ELAEW+LINEDGKLKLNRSLKVKSFLKGM+
Sbjct: 1   MDDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLKGMD 60

Query: 61  LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSR 120
           LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLDVHNLL R
Sbjct: 61  LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLDVHNLLGR 120

Query: 121 KVSISSQHDC 131
           K +ISSQHDC
Sbjct: 121 KAAISSQHDC 130

[Close]
BLAST of Moc01g32950 vs. NCBI nr
Match: KAG6581407.1 (Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 253.1 bits (645), Expect = 1.3e-63
Identity = 121/130 (93.08%), Postives = 126/130 (96.92%), Query Frame = 0 

Query: 1   MDDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGME 60
           MDDLS KKCVPCNS DLRPMTEDAAKHLI+ELAEW+LINEDGKLKLNRSLKVKSFLKGM+
Sbjct: 30  MDDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLKGMD 89

Query: 61  LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSR 120
           LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLDVHNLL R
Sbjct: 90  LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLDVHNLLGR 149

Query: 121 KVSISSQHDC 131
           K +ISSQHDC
Sbjct: 150 KAAISSQHDC 159

[Close]
BLAST of Moc01g32950 vs. NCBI nr
Match: XP_038881177.1 (pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 251.5 bits (641), Expect = 3.9e-63
Identity = 120/130 (92.31%), Postives = 127/130 (97.69%), Query Frame = 0 

Query: 1   MDDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGME 60
           MDDL+ KKCVPCNSNDLRPMTEDAAK LI+EL++W+L+NEDGKLKLNRSLKVKSFLKGME
Sbjct: 54  MDDLTTKKCVPCNSNDLRPMTEDAAKRLIRELSDWNLVNEDGKLKLNRSLKVKSFLKGME 113

Query: 61  LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSR 120
           LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI KLDVH+LLSR
Sbjct: 114 LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLDVHDLLSR 173

Query: 121 KVSISSQHDC 131
           KVSISSQHDC
Sbjct: 174 KVSISSQHDC 183

[Close]
BLAST of Moc01g32950 vs. NCBI nr
Match: XP_022925636.1 (uncharacterized protein LOC111432994 isoform X1 [Cucurbita moschata] >XP_023543298.1 uncharacterized protein LOC111803220 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 249.6 bits (636), Expect = 1.5e-62
Identity = 119/129 (92.25%), Postives = 125/129 (96.90%), Query Frame = 0 

Query: 2   DDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL 61
           +DLS KKCVPCNS DLRPMTEDAAKHLI+ELAEW+LINEDGKLKLNRSLKVKSFLKGM+L
Sbjct: 66  EDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLKGMDL 125

Query: 62  FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 121
           FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLDVHNLL RK
Sbjct: 126 FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLDVHNLLGRK 185

Query: 122 VSISSQHDC 131
            +ISSQHDC
Sbjct: 186 AAISSQHDC 194

[Close]
BLAST of Moc01g32950 vs. ExPASy Swiss-Prot
Match: Q6QJ72 (Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PDL2 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.0e-42
Identity = 78/119 (65.55%), Postives = 101/119 (84.87%), Query Frame = 0 

Query: 3   DLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMELF 62
           DLS KKCVPCN+ DLR MTE +A+ L+Q++A W L N++  LKL+RS +VKSF KG++ F
Sbjct: 65  DLSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLANDNDTLKLHRSWRVKSFTKGLDFF 124

Query: 63  KLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 122
           + +A++AE+EGHHPDLHLVGWNNV IEIWTHA+GGLTENDFILA+KI++L V +LL +K
Sbjct: 125 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKK 183

[Close]
BLAST of Moc01g32950 vs. ExPASy Swiss-Prot
Match: Q60BH8 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=MCA0497 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.4e-18
Identity = 42/109 (38.53%), Postives = 70/109 (64.22%), Query Frame = 0 

Query: 4   LSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMELFK 63
           L+AK+C PC    + P+T + A+ L+  +  W L  +D   KL R+ + ++F++ ++  +
Sbjct: 6   LTAKQCTPCQGG-IPPLTAEEAEKLLVHVPRWEL--KDAATKLKRTFRFENFMEALDFAR 65

Query: 64  LIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKL 113
            + E+ EAEGHHPD+  +GW    +E  TH + GL ENDFI+A+K+D+L
Sbjct: 66  KVGELCEAEGHHPDIG-IGWGYCRVEFQTHKINGLHENDFIMAAKVDEL 110

[Close]
BLAST of Moc01g32950 vs. ExPASy Swiss-Prot
Match: Q3B6N5 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Pelodictyon luteolum (strain DSM 273 / 2530) OX=319225 GN=Plut_0106 PE=3 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 3.0e-18
Identity = 45/112 (40.18%), Postives = 71/112 (63.39%), Query Frame = 0 

Query: 1   MDDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGME 60
           M +L+  KCV C S  L P+ E  ++ L++E+ EW +++EDG  +L R+   ++F + M 
Sbjct: 1   MGELNKTKCVSC-SEGLPPLAERESEELLKEIPEWVIVSEDGVSRLVRTFTFENFREAMA 60

Query: 61  LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKL 113
               + E+AE+E HHP L +  W  V +E WTHAV GL  NDF++A++ D+L
Sbjct: 61  FAGSVGELAESEQHHPKL-VTEWGKVRVEWWTHAVHGLHMNDFVMAARTDEL 110

[Close]
BLAST of Moc01g32950 vs. ExPASy Swiss-Prot
Match: Q8KFI4 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=CT0342 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 5.2e-18
Identity = 45/112 (40.18%), Postives = 62/112 (55.36%), Query Frame = 0 

Query: 1   MDDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGME 60
           M  L  K CVPC      PM  +  +  +  L EW L+++ G  KL R    K F   ++
Sbjct: 1   MTQLENKHCVPCEGT-AAPMASEELQRQLSSLPEWTLVDDSGTSKLVRVFTFKDFQSALD 60

Query: 61  LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKL 113
               + ++AEAEGHHP L L  W  VT+  WTHA+GG+  ND I+A+K +KL
Sbjct: 61  FTNRVGQLAEAEGHHPAL-LTEWGKVTVSWWTHAIGGIHLNDVIMATKTEKL 110

[Close]
BLAST of Moc01g32950 vs. ExPASy Swiss-Prot
Match: A6TVX6 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Alkaliphilus metalliredigens (strain QYMF) OX=293826 GN=Amet_4266 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 6.8e-18
Identity = 46/112 (41.07%), Postives = 71/112 (63.39%), Query Frame = 0 

Query: 1   MDDLSAKKCVPCNSNDLRPMTEDAAKHLIQEL-AEWHLINEDGKLKLNRSLKVKSFLKGM 60
           M++L+ KKC+PC S    P++ D  K  I +L  EW +IN+     L R  K K+F + +
Sbjct: 1   MNNLAEKKCIPC-SLGTPPLSSDEIKRYISQLHEEWKVINDH---HLEREFKFKNFKEAL 60

Query: 61  ELFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDK 112
               +I ++AE EGHHPD+ L+ W  V I ++TH + GL+E+DF+ A+K+DK
Sbjct: 61  SYTNVIGQLAEKEGHHPDM-LLSWGKVKITLFTHKIDGLSESDFVFAAKVDK 107

[Close]
BLAST of Moc01g32950 vs. ExPASy TrEMBL
Match: A0A6J1CJT1 (4a-hydroxytetrahydrobiopterin dehydratase OS=Momordica charantia OX=3673 GN=LOC111012265 PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.6e-67
Identity = 128/129 (99.22%), Postives = 129/129 (100.00%), Query Frame = 0 

Query: 2   DDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL 61
           +DLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL
Sbjct: 64  EDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL 123

Query: 62  FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 121
           FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK
Sbjct: 124 FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 183

Query: 122 VSISSQHDC 131
           VSISSQHDC
Sbjct: 184 VSISSQHDC 192

[Close]
BLAST of Moc01g32950 vs. ExPASy TrEMBL
Match: A0A6J1ECR9 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111432994 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 6.5e-64
Identity = 121/130 (93.08%), Postives = 126/130 (96.92%), Query Frame = 0 

Query: 1   MDDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGME 60
           MDDLS KKCVPCNS DLRPMTEDAAKHLI+ELAEW+LINEDGKLKLNRSLKVKSFLKGM+
Sbjct: 1   MDDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLKGMD 60

Query: 61  LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSR 120
           LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLDVHNLL R
Sbjct: 61  LFKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLDVHNLLGR 120

Query: 121 KVSISSQHDC 131
           K +ISSQHDC
Sbjct: 121 KAAISSQHDC 130

[Close]
BLAST of Moc01g32950 vs. ExPASy TrEMBL
Match: A0A6J1ECR6 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111432994 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.2e-63
Identity = 119/129 (92.25%), Postives = 125/129 (96.90%), Query Frame = 0 

Query: 2   DDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL 61
           +DLS KKCVPCNS DLRPMTEDAAKHLI+ELAEW+LINEDGKLKLNRSLKVKSFLKGM+L
Sbjct: 66  EDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLKGMDL 125

Query: 62  FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 121
           FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLDVHNLL RK
Sbjct: 126 FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLDVHNLLGRK 185

Query: 122 VSISSQHDC 131
            +ISSQHDC
Sbjct: 186 AAISSQHDC 194

[Close]
BLAST of Moc01g32950 vs. ExPASy TrEMBL
Match: A0A6J1EC79 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111432994 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.2e-63
Identity = 119/129 (92.25%), Postives = 125/129 (96.90%), Query Frame = 0 

Query: 2   DDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL 61
           +DLS KKCVPCNS DLRPMTEDAAKHLI+ELAEW+LINEDGKLKLNRSLKVKSFLKGM+L
Sbjct: 45  EDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLKGMDL 104

Query: 62  FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 121
           FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLDVHNLL RK
Sbjct: 105 FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLDVHNLLGRK 164

Query: 122 VSISSQHDC 131
            +ISSQHDC
Sbjct: 165 AAISSQHDC 173

[Close]
BLAST of Moc01g32950 vs. ExPASy TrEMBL
Match: A0A6J1IPK6 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111478787 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.7e-62
Identity = 118/129 (91.47%), Postives = 125/129 (96.90%), Query Frame = 0 

Query: 2   DDLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMEL 61
           +DLS KKCVPCNS DLRPMTEDAAKHLI+ELAEW+LINEDGKLKLNRSLKVKSFLKGM+L
Sbjct: 45  EDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLKGMDL 104

Query: 62  FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 121
           FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLDVH+LL RK
Sbjct: 105 FKLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLDVHHLLGRK 164

Query: 122 VSISSQHDC 131
            +ISSQHDC
Sbjct: 165 AAISSQHDC 173

[Close]
BLAST of Moc01g32950 vs. TAIR 10
Match: AT1G29810.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 174.1 bits (440), Expect = 7.4e-44
Identity = 78/119 (65.55%), Postives = 101/119 (84.87%), Query Frame = 0 

Query: 3   DLSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLINEDGKLKLNRSLKVKSFLKGMELF 62
           DLS KKCVPCN+ DLR MTE +A+ L+Q++A W L N++  LKL+RS +VKSF KG++ F
Sbjct: 65  DLSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLANDNDTLKLHRSWRVKSFTKGLDFF 124

Query: 63  KLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 122
           + +A++AE+EGHHPDLHLVGWNNV IEIWTHA+GGLTENDFILA+KI++L V +LL +K
Sbjct: 125 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKK 183

[Close]
BLAST of Moc01g32950 vs. TAIR 10
Match: AT5G51110.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 89.4 bits (220), Expect = 2.4e-18
Identity = 49/119 (41.18%), Postives = 74/119 (62.18%), Query Frame = 0 

Query: 4   LSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLI-NEDGKLKLNRSLKVKSFLKGMELF 63
           LS  +C P +S+   P++ D A+ L+ ++  W ++ NE G LK+    KV+ F  G+EL 
Sbjct: 99  LSQLQCSPVSSSQ-PPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELI 158

Query: 64  KLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 122
             I +VAEA GH+P LHL     V  E++T ++GGL+ NDFI+A+KID +   +L  RK
Sbjct: 159 NRIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLSPRK 216

[Close]
BLAST of Moc01g32950 vs. TAIR 10
Match: AT5G51110.2 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 89.4 bits (220), Expect = 2.4e-18
Identity = 49/119 (41.18%), Postives = 74/119 (62.18%), Query Frame = 0 

Query: 4   LSAKKCVPCNSNDLRPMTEDAAKHLIQELAEWHLI-NEDGKLKLNRSLKVKSFLKGMELF 63
           LS  +C P +S+   P++ D A+ L+ ++  W ++ NE G LK+    KV+ F  G+EL 
Sbjct: 72  LSQLQCSPVSSSQ-PPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELI 131

Query: 64  KLIAEVAEAEGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKIDKLDVHNLLSRK 122
             I +VAEA GH+P LHL     V  E++T ++GGL+ NDFI+A+KID +   +L  RK
Sbjct: 132 NRIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLSPRK 189

The following BLAST results are available for this feature:
BLAST of Moc01g32950 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022142040.13.4e-6799.22uncharacterized protein LOC111012265 [Momordica charantia][more]
XP_022925639.11.3e-6393.08uncharacterized protein LOC111432994 isoform X3 [Cucurbita moschata] >XP_0235433... [more]
KAG6581407.11.3e-6393.08Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial, partial [Cucurbita ar... [more]
XP_038881177.13.9e-6392.31pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial isoform X2 [Benincasa ... [more]
XP_022925636.11.5e-6292.25uncharacterized protein LOC111432994 isoform X1 [Cucurbita moschata] >XP_0235432... [more]
BLAST of Moc01g32950 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q6QJ721.0e-4265.55Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thalian... [more]
Q60BH81.4e-1838.53Putative pterin-4-alpha-carbinolamine dehydratase OS=Methylococcus capsulatus (s... [more]
Q3B6N53.0e-1840.18Putative pterin-4-alpha-carbinolamine dehydratase OS=Pelodictyon luteolum (strai... [more]
Q8KFI45.2e-1840.18Putative pterin-4-alpha-carbinolamine dehydratase OS=Chlorobaculum tepidum (stra... [more]
A6TVX66.8e-1841.07Putative pterin-4-alpha-carbinolamine dehydratase OS=Alkaliphilus metalliredigen... [more]
BLAST of Moc01g32950 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CJT11.6e-6799.224a-hydroxytetrahydrobiopterin dehydratase OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1ECR96.5e-6493.084a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1ECR67.2e-6392.254a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1EC797.2e-6392.254a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IPK62.7e-6291.474a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
BLAST of Moc01g32950 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G29810.17.4e-4465.55Transcriptional coactivator/pterin dehydratase [more]
AT5G51110.12.4e-1841.18Transcriptional coactivator/pterin dehydratase [more]
AT5G51110.22.4e-1841.18Transcriptional coactivator/pterin dehydratase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036428Pterin 4 alpha carbinolamine dehydratase superfamilyGENE3D3.30.1360.20Transcriptional coactivator/pterin dehydratasecoord: 19..116
e-value: 1.9E-27
score: 97.0
IPR036428Pterin 4 alpha carbinolamine dehydratase superfamilySUPERFAMILY55248PCD-likecoord: 18..112
IPR001533Pterin 4 alpha carbinolamine dehydratasePFAMPF01329Pterin_4acoord: 19..112
e-value: 5.4E-27
score: 93.7
IPR001533Pterin 4 alpha carbinolamine dehydratasePANTHERPTHR12599PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASEcoord: 3..123
NoneNo IPR availablePANTHERPTHR12599:SF0PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASEcoord: 3..123
NoneNo IPR availableCDDcd00913PCD_DCoH_subfamily_acoord: 34..112
e-value: 1.61912E-33
score: 110.396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g32950.1Moc01g32950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006729 tetrahydrobiopterin biosynthetic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0008124 4-alpha-hydroxytetrahydrobiopterin dehydratase activity