Moc01g32420 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g32420
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionUbiquitin-like modifier-activating enzyme ATG7
Locationchr1: 22820277 .. 22824554 (+)
RNA-Seq ExpressionMoc01g32420
SyntenyMoc01g32420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCTAACGATAAGGGTTCTATTTTGCAATTCGCACCATTCCAAAGCTCAGTTGATGAAGGATTCTGGCATAGACTCTCTTCCTTGAAGCTCAACGAGCTTGGCATCGACGATTCCCCTATTCCCATCACTGGTCTGCTTCTTATTTCTTTGGTTCTTTCTTTCTTTCTTTCTTTTCTGTGATTCCCAATTATTAGCGGCAAGCAATTTTCTTCTTGATGGTTTCGGAGTTCAGATGATCTTGTGACTAACTATCAGTAAAAATCAAACTCTTTCACGAGGAAAGAATGTTGGTTAATCAATGTGATTAAGCTGAACAATATATTTGACGAGCATACAGTTTGAATTTGAATTCTTTGTTTTGGGCATTCTTTAAATTCAAACTTTTTAACTATGTGATCTGGTTTTGCTTGTATTATAATGCTTTATCTTTATCTTTTGTTCATGCATTAGCCATCTTTATATAGCCTATTTTGGTTACTCATAGCGTGTGTATAAAATAATCAAGAACCATCTCCTTGTAGGTTTTTACGCACCTTGTTCACATTCACAAGTGTCAAATCATATGACACTTCTTTCTGAGTCATTGCCTTCCGAAGCAAGCAGGCAACCGTCGGCACCATCAACTAGTACAGGTAACAGGAATAGGTGCTCTGTTCCTGGAATTCTGTACAATACAAATACAGTCGAAAGCTTCCATGCCCTTGACAAGCAAAGCTTACTAAAGTCTGAAGCAAAGAAGGTATATTACGCTCAAATGATTTAATATCTTGAATTATACACGGGTAATGGCTATTTCTATTTCTTGTTTAGGATAAATTTATTATGTTAGAAACTCTTGCGGATTGTTTGCGTGATGTTTGTGTTACAAGTTCAGTTTTCATTGTTGTGAGTAGATTCGGGAGGATATTCTCAGTGGAAAAGCTATAGAGGATAGTTCAGTACTGGCAAGGTTCCTTTTGATCTCATTTGCTGACTTGAAAAAATGGAACTTTTACTATTGGTTTGCTTTCCCTGCTCTTGTTCTTGATCCTCCAGCAACTGTAGTTGAACTGCAGCCTGCTTCACATTGGCTTAACTCAAAAGAGGTACTTAAAAGTGGTATTTTGTTGTTAAAGTGTTTGAATAGCTATACGTTTTTTATATCTATGTTTCATTTTAATATATAGGCTGAATCAGTTTCCTCAGCTTTTGGTCAGTGGCGCAGCTCGGACCTAACCTCTGGTAGGCTTGCATTCTTCACTGTTTAGAGTTTTGTAAATTTTCATGTCCAGTTTTTGTTGTTTACAGTCTAATTTGCATTCTGCTGCAGATATACCATTCTTCTTGGTGCATATTGATTCGAGTTCTCAAGCTAGCATTAAACATCTAACAGATTTTGAAACCTTCGAGAGCAATGGGGAAAAGGTCTGTTGGCATTACTCTTCCAGTTAGTGGGAATGGGAAAAAATGCTTGCTTCTAGAGAAAATTAGATCTGCTGGATTGTTTGGTGATTTAAACTGTTATGAACTCCCATTACCATACACTTATTGATGAAACTTTCTTAAAGCCAGTTTAGTCTCTTTTTGCAGCTTCTTTTTGGTTTTTATGACCCTTGTCATCTTCCAAATAATCCTGGCTGGCCTCTTCGCAACTTCCTAGCTCTCATTTACTCAAGATGGAATCTGAAGTCAGTTAACTTTTTATGCTATAGAGAGAACCGTGGTTTTGCAGATTTAACACTATCCCTTGTTGGAAAAGCCCTGATTGATGCTCCACAAGGTAGAGTCTAAGAAATTTGTAACAGTATCAATTTTATCTCTACTCACCGAGGAGGTAGCTTATCTTATATTTTGTTGATTTCTAGGGTTGATAGATCAAAGGTGCATGCCTAATGCAGTTGGATGGGAACTCAACCGAGGGAAGAAATCTTGTAGATCTATTAACCTTGCAAAATCCATGGACCCAACTAGGTATATTTTTATGCACATGCTGTTTTGGTTCGGGGAGTCCTCATCTAATTATTGACTCAATACATATATTGTTGACAGGTTAGCTATAACAGCAGCTGATTTGAATTTGAGATTAATGAGATGGCGTGCTTTGCCATCTTTAAACTTGAACATCTTGTCATCTCTCAAGTGTCTCTTGCTAGGGGCTGGTACTCTTGGATGTCAGGTTGCTCGTATGCTTATGGTGAGTGCTTCATAGAAAGTTGTAGTTTAGATTTCTCTCTCTTTGTTGATGAAGGTAACTTATTCGTAGGCATGGGGCATACGGAAAATTACACTAGTTGACAGTGGAAGGGTAGCTATGTCTAATCCGTTGAGGCAATCCCTTTACACATTGGAGGACTGCCTTAATGGTGGTGACTTTAAAGCCAAAGCAGCAGTTAAAAGTCTTGATCGAATATTTCCTGGCATGGTAAAATTTTGATGCATGACCCCAACAGAATGAAAATTGCCTGTTTTATAATTTGATTCATTTGTGTGTTTTTTTTTTATTTTAAAAATACATAGGAAGCTGAAGGTGTTGTGATTTCCATACCAATGCCTGGGCATCCTGTTCCAGACCATGAAGTAGCCAGCATGATTGATGATTGTAGACGCCTTGATGATTTAATTAATTCTCATGATGCAGTTTTCTTGTTGACTGATACTAGGGAAAGTCGGTGGCTTCCTACGCTTTTATGTGCCAATACAAATAAGGTATAATTTTGTCAAAGAAATTGTTTGTATAGGTTTCTCGTACTTGTCTTAATGTAATGTCTGTTTCTGGTTTGAAGGTTACAATCAATGCAGCTTTGGGGTTTGATAGTTTCTTAGTTATGCGCCATGGAGCTGGTCCTTCTGGTTCCTGTCATGACGCAACATCTCAAATTAATATGGCTAACCTTTCCTTAAATGCAACAGACAGAAGGCAGAGATTAGGCTGTTACTTCTGTAATGATGTTGTTGCACCCATTGATGTAATTTTCTCAAGCTCACGTGATGTATTTTGTTGATTTTTTTTTTTTTTTTTGTGTCGTGTTTATTTTTGATTCTCATGCTTTTATTGTAGTCAACTACCAATCGCACATTGGACCAGCAATGCACTGTTACTCGCCCAGGGCTGGCTCCCATCGCATCATCGATTGCAGTTGAACTTCTTGTCGGAATTTTGCATCACCCTGAGGGGTAGGTTCTCTTATACATTGGTGATTAAGGCTTGAGATTTGTCTGTGGCTCGACTTTTGAAATTATGCTTAGGTTGATACTACTAGTCGAGTTAATTAGAGTTCTCATACTGAGTTTCAGAGGTGCCTAACAGTGGCTTACTGGTTTTTACCCCAAATTGTACAGAATATACGCTGAAGGTGAACTCTTAAACTCTGGGATCGATGGAACTTCTGAGCAGCCTCTGGGTATCTTGCCCCATCAAATTCGAGGCTCCCTCTCACAGTTTTCTCAGATGACACTTGTGGGCCGCTCATCTAACAGTTGTACAGCTTGCTCCAGCACGGTAAGTTATTCTGCCTGCTCATTTCTGCCATATTTCTTATTTGTCCCTTTACAGATAACATAATATTTTGTATGACACATACTTTAAACTTATATTCTGATTTCAATTTGCGTTGAACCTTGACGTTTGGGTTCTTCTGAGACTAGCCTAACCAAACTAATTAGGGATCTCAAAGAAAGTGGATTCAAGTATTTTGCCCGCTCTTACCAATCATCTAATTACTTCGGACACTTGAATGGTCATGCAGTTTGATTAGGTGAATGTTTAATGTGATCCAACCGTCCATATGAAAATTTCTTCCAACACTGTCACTGCCCTCTTAAACTCTCGAATCATACTCGTTCAAAGATTATCGAATTTAATCGACAATGTAGTGGCTACAACTCGAGCCCCCTAGAAACCAGCCATGTAGGTCAAATATGGAAAGAGTTGGTGTAGGATGGTGTCATTTGGCCATAGTCTAGAGAGACAAGAGATGGGTGTAGCCTAAATCTGTTGGATAAGTTTCTTTCATTTTTCTGAACCTGTTTTGAATGCTCCTTGGATTATATCTTTCCCTTCTTTTACTGTCAAGCATCAAGCTTTATTTGATTCATGGTGAACTTTTGCAGGTTGTGTCGGAATATCGCAATAGGGGCATGGATTTTATTCTTGAAGCGATTAACCATCCAACTTATTTGGAGGATTTGACCGGACTTACAGAGTTGATGAAATCAACTAGCTCTTTCCAACTGGACTGGGATAATGATACAGATGGAAGTGATGAAGATAATGATGGATGTGTCGAAATTTGA

mRNA sequence

ATGGCCGCTAACGATAAGGGTTCTATTTTGCAATTCGCACCATTCCAAAGCTCAGTTGATGAAGGATTCTGGCATAGACTCTCTTCCTTGAAGCTCAACGAGCTTGGCATCGACGATTCCCCTATTCCCATCACTGGTTTTTACGCACCTTGTTCACATTCACAAGTGTCAAATCATATGACACTTCTTTCTGAGTCATTGCCTTCCGAAGCAAGCAGGCAACCGTCGGCACCATCAACTAGTACAGGTAACAGGAATAGGTGCTCTGTTCCTGGAATTCTGTACAATACAAATACAGTCGAAAGCTTCCATGCCCTTGACAAGCAAAGCTTACTAAAGTCTGAAGCAAAGAAGATTCGGGAGGATATTCTCAGTGGAAAAGCTATAGAGGATAGTTCAGTACTGGCAAGGTTCCTTTTGATCTCATTTGCTGACTTGAAAAAATGGAACTTTTACTATTGGTTTGCTTTCCCTGCTCTTGTTCTTGATCCTCCAGCAACTGTAGTTGAACTGCAGCCTGCTTCACATTGGCTTAACTCAAAAGAGGCTGAATCAGTTTCCTCAGCTTTTGGTCAGTGGCGCAGCTCGGACCTAACCTCTGATATACCATTCTTCTTGGTGCATATTGATTCGAGTTCTCAAGCTAGCATTAAACATCTAACAGATTTTGAAACCTTCGAGAGCAATGGGGAAAAGCTTCTTTTTGGTTTTTATGACCCTTGTCATCTTCCAAATAATCCTGGCTGGCCTCTTCGCAACTTCCTAGCTCTCATTTACTCAAGATGGAATCTGAAGTCAGTTAACTTTTTATGCTATAGAGAGAACCGTGGTTTTGCAGATTTAACACTATCCCTTGTTGGAAAAGCCCTGATTGATGCTCCACAAGGGTTGATAGATCAAAGGTGCATGCCTAATGCAGTTGGATGGGAACTCAACCGAGGGAAGAAATCTTGTAGATCTATTAACCTTGCAAAATCCATGGACCCAACTAGGTTAGCTATAACAGCAGCTGATTTGAATTTGAGATTAATGAGATGGCGTGCTTTGCCATCTTTAAACTTGAACATCTTGTCATCTCTCAAGTGTCTCTTGCTAGGGGCTGGTACTCTTGGATGTCAGGTTGCTCGTATGCTTATGGCATGGGGCATACGGAAAATTACACTAGTTGACAGTGGAAGGGTAGCTATGTCTAATCCGTTGAGGCAATCCCTTTACACATTGGAGGACTGCCTTAATGGTGGTGACTTTAAAGCCAAAGCAGCAGTTAAAAGTCTTGATCGAATATTTCCTGGCATGGAAGCTGAAGGTGTTGTGATTTCCATACCAATGCCTGGGCATCCTGTTCCAGACCATGAAGTAGCCAGCATGATTGATGATTGTAGACGCCTTGATGATTTAATTAATTCTCATGATGCAGTTTTCTTGTTGACTGATACTAGGGAAAGTCGGTGGCTTCCTACGCTTTTATGTGCCAATACAAATAAGGTTACAATCAATGCAGCTTTGGGGTTTGATAGTTTCTTAGTTATGCGCCATGGAGCTGGTCCTTCTGGTTCCTGTCATGACGCAACATCTCAAATTAATATGGCTAACCTTTCCTTAAATGCAACAGACAGAAGGCAGAGATTAGGCTGTTACTTCTGTAATGATGTTGTTGCACCCATTGATTCAACTACCAATCGCACATTGGACCAGCAATGCACTGTTACTCGCCCAGGGCTGGCTCCCATCGCATCATCGATTGCAGTTGAACTTCTTGTCGGAATTTTGCATCACCCTGAGGGAATATACGCTGAAGGTGAACTCTTAAACTCTGGGATCGATGGAACTTCTGAGCAGCCTCTGGGTATCTTGCCCCATCAAATTCGAGGCTCCCTCTCACAGTTTTCTCAGATGACACTTGTGGGCCGCTCATCTAACAGTTGTACAGCTTGCTCCAGCACGGTTGTGTCGGAATATCGCAATAGGGGCATGGATTTTATTCTTGAAGCGATTAACCATCCAACTTATTTGGAGGATTTGACCGGACTTACAGAGTTGATGAAATCAACTAGCTCTTTCCAACTGGACTGGGATAATGATACAGATGGAAGTGATGAAGATAATGATGGATGTGTCGAAATTTGA

Coding sequence (CDS)

ATGGCCGCTAACGATAAGGGTTCTATTTTGCAATTCGCACCATTCCAAAGCTCAGTTGATGAAGGATTCTGGCATAGACTCTCTTCCTTGAAGCTCAACGAGCTTGGCATCGACGATTCCCCTATTCCCATCACTGGTTTTTACGCACCTTGTTCACATTCACAAGTGTCAAATCATATGACACTTCTTTCTGAGTCATTGCCTTCCGAAGCAAGCAGGCAACCGTCGGCACCATCAACTAGTACAGGTAACAGGAATAGGTGCTCTGTTCCTGGAATTCTGTACAATACAAATACAGTCGAAAGCTTCCATGCCCTTGACAAGCAAAGCTTACTAAAGTCTGAAGCAAAGAAGATTCGGGAGGATATTCTCAGTGGAAAAGCTATAGAGGATAGTTCAGTACTGGCAAGGTTCCTTTTGATCTCATTTGCTGACTTGAAAAAATGGAACTTTTACTATTGGTTTGCTTTCCCTGCTCTTGTTCTTGATCCTCCAGCAACTGTAGTTGAACTGCAGCCTGCTTCACATTGGCTTAACTCAAAAGAGGCTGAATCAGTTTCCTCAGCTTTTGGTCAGTGGCGCAGCTCGGACCTAACCTCTGATATACCATTCTTCTTGGTGCATATTGATTCGAGTTCTCAAGCTAGCATTAAACATCTAACAGATTTTGAAACCTTCGAGAGCAATGGGGAAAAGCTTCTTTTTGGTTTTTATGACCCTTGTCATCTTCCAAATAATCCTGGCTGGCCTCTTCGCAACTTCCTAGCTCTCATTTACTCAAGATGGAATCTGAAGTCAGTTAACTTTTTATGCTATAGAGAGAACCGTGGTTTTGCAGATTTAACACTATCCCTTGTTGGAAAAGCCCTGATTGATGCTCCACAAGGGTTGATAGATCAAAGGTGCATGCCTAATGCAGTTGGATGGGAACTCAACCGAGGGAAGAAATCTTGTAGATCTATTAACCTTGCAAAATCCATGGACCCAACTAGGTTAGCTATAACAGCAGCTGATTTGAATTTGAGATTAATGAGATGGCGTGCTTTGCCATCTTTAAACTTGAACATCTTGTCATCTCTCAAGTGTCTCTTGCTAGGGGCTGGTACTCTTGGATGTCAGGTTGCTCGTATGCTTATGGCATGGGGCATACGGAAAATTACACTAGTTGACAGTGGAAGGGTAGCTATGTCTAATCCGTTGAGGCAATCCCTTTACACATTGGAGGACTGCCTTAATGGTGGTGACTTTAAAGCCAAAGCAGCAGTTAAAAGTCTTGATCGAATATTTCCTGGCATGGAAGCTGAAGGTGTTGTGATTTCCATACCAATGCCTGGGCATCCTGTTCCAGACCATGAAGTAGCCAGCATGATTGATGATTGTAGACGCCTTGATGATTTAATTAATTCTCATGATGCAGTTTTCTTGTTGACTGATACTAGGGAAAGTCGGTGGCTTCCTACGCTTTTATGTGCCAATACAAATAAGGTTACAATCAATGCAGCTTTGGGGTTTGATAGTTTCTTAGTTATGCGCCATGGAGCTGGTCCTTCTGGTTCCTGTCATGACGCAACATCTCAAATTAATATGGCTAACCTTTCCTTAAATGCAACAGACAGAAGGCAGAGATTAGGCTGTTACTTCTGTAATGATGTTGTTGCACCCATTGATTCAACTACCAATCGCACATTGGACCAGCAATGCACTGTTACTCGCCCAGGGCTGGCTCCCATCGCATCATCGATTGCAGTTGAACTTCTTGTCGGAATTTTGCATCACCCTGAGGGAATATACGCTGAAGGTGAACTCTTAAACTCTGGGATCGATGGAACTTCTGAGCAGCCTCTGGGTATCTTGCCCCATCAAATTCGAGGCTCCCTCTCACAGTTTTCTCAGATGACACTTGTGGGCCGCTCATCTAACAGTTGTACAGCTTGCTCCAGCACGGTTGTGTCGGAATATCGCAATAGGGGCATGGATTTTATTCTTGAAGCGATTAACCATCCAACTTATTTGGAGGATTTGACCGGACTTACAGAGTTGATGAAATCAACTAGCTCTTTCCAACTGGACTGGGATAATGATACAGATGGAAGTGATGAAGATAATGATGGATGTGTCGAAATTTGA

Protein sequence

MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHMTLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIREDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNSKEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDPCHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQRCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSLKCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKAAVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTRESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRRQRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEGELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDFILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI
Homology
BLAST of Moc01g32420 vs. NCBI nr
Match: XP_022142296.1 (ubiquitin-like modifier-activating enzyme atg7 [Momordica charantia])

HSP 1 Score: 1425.2 bits (3688), Expect = 0.0e+00
Identity = 708/708 (100.00%), Postives = 708/708 (100.00%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60
           MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM
Sbjct: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60

Query: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120
           TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR
Sbjct: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120

Query: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180
           EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS
Sbjct: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180

Query: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240
           KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP
Sbjct: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240

Query: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300
           CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ
Sbjct: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300

Query: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360
           RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL
Sbjct: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360

Query: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420
           KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA
Sbjct: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420

Query: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480
           AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR
Sbjct: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480

Query: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540
           ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR
Sbjct: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540

Query: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600
           QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG
Sbjct: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600

Query: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660
           ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF
Sbjct: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660

Query: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 709
           ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI
Sbjct: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 708

BLAST of Moc01g32420 vs. NCBI nr
Match: XP_008439746.1 (PREDICTED: ubiquitin-like modifier-activating enzyme atg7 [Cucumis melo])

HSP 1 Score: 1295.0 bits (3350), Expect = 0.0e+00
Identity = 640/708 (90.40%), Postives = 674/708 (95.20%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60
           MAANDKGSILQFAPFQS+VDEGFWHRLSSLKLN+LGIDDSPIPITGF+APCSHSQVSNH+
Sbjct: 1   MAANDKGSILQFAPFQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSQVSNHL 60

Query: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120
           TLLSESLP+E  R  S P T+ GNRNRC+VPGILYNTNTVESFHAL+K SLLKSEAKKI 
Sbjct: 61  TLLSESLPTEVKRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALEKLSLLKSEAKKIW 120

Query: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180
           EDILSGKA+EDSSVLARFLLISFADLKKWNF+YWFAFPALVLDPPATVVEL+ AS W N 
Sbjct: 121 EDILSGKALEDSSVLARFLLISFADLKKWNFHYWFAFPALVLDPPATVVELKSASQWFNL 180

Query: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240
           +EAES+S+AFGQWRSSDLTSDIPFFLV IDS+SQASIKHL DFET ++NGEKLLFGFYDP
Sbjct: 181 EEAESLSTAFGQWRSSDLTSDIPFFLVIIDSNSQASIKHLRDFETCQNNGEKLLFGFYDP 240

Query: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300
           CHLP+NPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALID P+GL DQ
Sbjct: 241 CHLPSNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDPKGLRDQ 300

Query: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360
            CMPNAVGWELN+GKK  ++INLAKSMDPTRLAI+AADLNLRLMRWRALPSLN+NILSSL
Sbjct: 301 CCMPNAVGWELNKGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSL 360

Query: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420
           KCLLLGAGTLGCQVARMLMAWG+RKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKA+A
Sbjct: 361 KCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAEA 420

Query: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480
           AVKSL+RIFP MEAEGVVISIPMPGHPVPDHE AS IDDCRRLDDLINSHDAVFLLTDTR
Sbjct: 421 AVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAVFLLTDTR 480

Query: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540
           ESRWLPTLLCAN NKVTI AALGFDSFLVMRHGAGPSGSCHD+TSQ N+ANLSLNAT+ R
Sbjct: 481 ESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNATNTR 540

Query: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600
           QRLGCYFCNDVVAPIDST NRTLDQQCTVTRPGLAPIAS+IAVELLVGILHHPEGIYAEG
Sbjct: 541 QRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEG 600

Query: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660
           ELLNSGIDG SEQPLGILPHQIRG LSQFSQMTLVGRSSNSCTACSS VVSEYRNRGMDF
Sbjct: 601 ELLNSGIDGASEQPLGILPHQIRGFLSQFSQMTLVGRSSNSCTACSSMVVSEYRNRGMDF 660

Query: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 709
           IL+AINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGS +D DGC+EI
Sbjct: 661 ILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSGDD-DGCIEI 707

BLAST of Moc01g32420 vs. NCBI nr
Match: XP_038880974.1 (ubiquitin-like modifier-activating enzyme atg7 [Benincasa hispida])

HSP 1 Score: 1287.7 bits (3331), Expect = 0.0e+00
Identity = 636/708 (89.83%), Postives = 673/708 (95.06%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60
           MAAN KGSILQFAPFQS+VDEGFWHRLSSLKLNELGIDDSPIPITGF+APCSHSQVSNH+
Sbjct: 1   MAANHKGSILQFAPFQSAVDEGFWHRLSSLKLNELGIDDSPIPITGFFAPCSHSQVSNHL 60

Query: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120
            LLSESLP++ +R+ SAPST+ GNRNRCSVPG LYNTNTVESFHALDK SLLKSEAKKI 
Sbjct: 61  ILLSESLPAKVTRESSAPSTTKGNRNRCSVPGTLYNTNTVESFHALDKLSLLKSEAKKIW 120

Query: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180
           EDILSGKA+EDSSVLARFLLISFADLKKWNF+YWFAFPALVL PPATVVEL+PASHW N 
Sbjct: 121 EDILSGKALEDSSVLARFLLISFADLKKWNFHYWFAFPALVLHPPATVVELKPASHWFNL 180

Query: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240
           +EAES+S+AFGQWRSSDLT DIPFFLV+IDSSSQA IKHL DFET ++ GEKLLFGFYDP
Sbjct: 181 EEAESLSTAFGQWRSSDLTCDIPFFLVNIDSSSQAIIKHLRDFETCQNTGEKLLFGFYDP 240

Query: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300
           CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGF DLTLSLVG+ALID P+GL DQ
Sbjct: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFPDLTLSLVGRALIDDPKGLRDQ 300

Query: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360
            CMPNAVGWELN+GKK  R+INLAKSMDPTRLAI+AADLNLRLMRWRALPSLN+N+LSSL
Sbjct: 301 CCMPNAVGWELNKGKKCYRTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINVLSSL 360

Query: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420
           KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNP+RQSLYTLEDCLNGGDFKA+A
Sbjct: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPMRQSLYTLEDCLNGGDFKAEA 420

Query: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480
           AVKSL+RIFP MEAEGVVISIPMPGHPVPDHE AS IDDCRRLDDLINSHDAVFLLTDTR
Sbjct: 421 AVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAVFLLTDTR 480

Query: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540
           ESRWLPTLLCANTNKVTI AALGFDSFLVMRHGAGPSGSCHD+ SQ N+ANLSL+AT+ R
Sbjct: 481 ESRWLPTLLCANTNKVTITAALGFDSFLVMRHGAGPSGSCHDSISQTNIANLSLSATNTR 540

Query: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600
           QRLGCYFCNDVVAPIDST NRTLDQQCTVTRPGLAPIAS+IAVELLVGILHHPEGIYAEG
Sbjct: 541 QRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEG 600

Query: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660
           ELLNSGI+G SEQPLGILPHQIRGSLSQFSQMTLVGRSS+SCTACSS VVSEYRNRGMDF
Sbjct: 601 ELLNSGINGASEQPLGILPHQIRGSLSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDF 660

Query: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 709
           IL+AINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGS +D D C+EI
Sbjct: 661 ILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSGDD-DECIEI 707

BLAST of Moc01g32420 vs. NCBI nr
Match: XP_022926466.1 (ubiquitin-like modifier-activating enzyme atg7 [Cucurbita moschata])

HSP 1 Score: 1282.7 bits (3318), Expect = 0.0e+00
Identity = 637/708 (89.97%), Postives = 670/708 (94.63%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60
           MAANDKGSILQFAPFQS VDEGFWHRLSSLKLNELGIDDSPIPITGF+APC+HSQVSNH+
Sbjct: 1   MAANDKGSILQFAPFQSLVDEGFWHRLSSLKLNELGIDDSPIPITGFFAPCAHSQVSNHL 60

Query: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120
           TLLSESLPSE S + SAP T+ GNRNRCSVPGILYNTNTVESFHALDK SLLKSEAKKI 
Sbjct: 61  TLLSESLPSEVSSESSAPLTTKGNRNRCSVPGILYNTNTVESFHALDKLSLLKSEAKKIW 120

Query: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180
           EDILSGKAIEDSSVLARFLLISFADLKKWNF+YWFAFPALVL+PPATVVEL+PASHW N 
Sbjct: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFHYWFAFPALVLNPPATVVELKPASHWFNL 180

Query: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240
           +EAESVSS+F QWRSS+LTSDIPFFLV+IDSSSQASIKHL DFE  ++NGEKLLFGFYDP
Sbjct: 181 EEAESVSSSFAQWRSSNLTSDIPFFLVNIDSSSQASIKHLRDFEACQNNGEKLLFGFYDP 240

Query: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300
           CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALID  QG  DQ
Sbjct: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDTQGFRDQ 300

Query: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360
           +CMPN VGWELNRGKK  +SINLAKSMDPTRLAI+AADLNLRLMRWRALPSLN+NILSSL
Sbjct: 301 QCMPNVVGWELNRGKKCHKSINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSL 360

Query: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420
           KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYT+EDCLNGGDFKA+A
Sbjct: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTMEDCLNGGDFKAEA 420

Query: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480
           AVKSL+RIFP MEAEGVVISIPMPGHPV D+E ASM+DDCRRLDDLINSHDAVFLLTDTR
Sbjct: 421 AVKSLNRIFPAMEAEGVVISIPMPGHPVSDNEAASMLDDCRRLDDLINSHDAVFLLTDTR 480

Query: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540
           ESRWLPTLLCANTNKVTI AALGFDSFLVMRHGAGPSGSCHD+TSQ N+ANLSLNA + +
Sbjct: 481 ESRWLPTLLCANTNKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNAANTK 540

Query: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600
           QRLGCYFCNDVVAPIDST NRTLDQQCTVTRPGLAPIAS+IAVELLVGILHHPEGIYAE 
Sbjct: 541 QRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEC 600

Query: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660
           EL NS   G SEQPLGILPHQIRGSLSQFSQMTLVGRSSN+CTACSS VVSEYRNRGMDF
Sbjct: 601 ELSNS---GASEQPLGILPHQIRGSLSQFSQMTLVGRSSNTCTACSSLVVSEYRNRGMDF 660

Query: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 709
           IL+AINHPTYLEDLTGLTELMKSTSSFQLDWDN TDGS +D+DGC+EI
Sbjct: 661 ILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNYTDGSGDDDDGCIEI 705

BLAST of Moc01g32420 vs. NCBI nr
Match: KAG6594550.1 (Ubiquitin-like modifier-activating enzyme atg7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026526.1 Ubiquitin-like modifier-activating enzyme atg7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1281.2 bits (3314), Expect = 0.0e+00
Identity = 636/708 (89.83%), Postives = 670/708 (94.63%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60
           MAANDKGSILQFAPFQS VDEGFWHRLSSLKLNELGIDDSPIPITGF+APC+HSQVSNH+
Sbjct: 1   MAANDKGSILQFAPFQSLVDEGFWHRLSSLKLNELGIDDSPIPITGFFAPCAHSQVSNHL 60

Query: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120
           TLLSESLPSE S + SAP T+ GNRNRCSVPGILYNTNTVESFHALDK SLLKSEAKKI 
Sbjct: 61  TLLSESLPSEVSSESSAPLTTKGNRNRCSVPGILYNTNTVESFHALDKLSLLKSEAKKIW 120

Query: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180
           EDILSGKAIEDSSVLARFLLISFADLKKWNF+YWFAFPALVL+PPATVVEL+PASHW N 
Sbjct: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFHYWFAFPALVLNPPATVVELKPASHWFNL 180

Query: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240
           +EAESVSS+F QWRSS+LTSDIPFFLV+IDSSSQASIKHL DFE  ++NGEKLLFGFYDP
Sbjct: 181 EEAESVSSSFAQWRSSNLTSDIPFFLVNIDSSSQASIKHLRDFEACQNNGEKLLFGFYDP 240

Query: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300
           CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLT SLVGKALID  QG  DQ
Sbjct: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTSSLVGKALIDDAQGFRDQ 300

Query: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360
           +CMPN VGWELNRGKK  +SINLAKSMDPTRLAI+AADLNLRLMRWRALPSLN+NILSSL
Sbjct: 301 QCMPNVVGWELNRGKKCHKSINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSL 360

Query: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420
           KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYT+EDCLNGGDFKA+A
Sbjct: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTMEDCLNGGDFKAEA 420

Query: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480
           AVKSL+RIFP MEAEGVVISIPMPGHPV D+E ASM+DDCRRLDDLINSHDAVFLLTDTR
Sbjct: 421 AVKSLNRIFPAMEAEGVVISIPMPGHPVSDNEAASMLDDCRRLDDLINSHDAVFLLTDTR 480

Query: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540
           ESRWLPTLLCANTNKVTI AALGFDSFLVMRHGAGPSGSCHD+TSQ N+ANLSLNAT+ +
Sbjct: 481 ESRWLPTLLCANTNKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNATNTK 540

Query: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600
           QRLGCYFCNDVVAPIDST NRTLDQQCTVTRPGLAPIAS+IAVELLVGILHHPEGIYAE 
Sbjct: 541 QRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEC 600

Query: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660
           EL NS   G SEQPLGILPHQIRGSLSQFSQMTLVGRSSN+CTACSS VVSEYRNRGMDF
Sbjct: 601 ELSNS---GASEQPLGILPHQIRGSLSQFSQMTLVGRSSNTCTACSSLVVSEYRNRGMDF 660

Query: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 709
           IL+AINHPTYLEDLTGLTELMKST+SFQLDWDN TDGS +D+DGC+EI
Sbjct: 661 ILQAINHPTYLEDLTGLTELMKSTNSFQLDWDNYTDGSGDDDDGCIEI 705

BLAST of Moc01g32420 vs. ExPASy Swiss-Prot
Match: Q94CD5 (Ubiquitin-like modifier-activating enzyme atg7 OS=Arabidopsis thaliana OX=3702 GN=ATG7 PE=1 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 6.6e-285
Identity = 492/695 (70.79%), Postives = 569/695 (81.87%), Query Frame = 0

Query: 9   ILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHMTLLSESLP 68
           ILQFAP  SSVDEGFWH  SSLKL++LGIDDSPI ITGFY PC H QVSNH+TLLSESLP
Sbjct: 10  ILQFAPLNSSVDEGFWHSFSSLKLDKLGIDDSPISITGFYGPCGHPQVSNHLTLLSESLP 69

Query: 69  SEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIREDILSGKA 128
            +   Q    STS GNRN+C VPGILYNTNTVESF+ LDKQSLLK+EA KI EDI SGKA
Sbjct: 70  LD--EQSLIASTSHGNRNKCPVPGILYNTNTVESFNKLDKQSLLKAEANKIWEDIQSGKA 129

Query: 129 IEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNSKEAESVSS 188
           +ED SVL RFL+ISFADLKKW+F YWFAFPA VLDPP +++EL+PAS + +S+EAESVS+
Sbjct: 130 LEDPSVLPRFLVISFADLKKWSFRYWFAFPAFVLDPPVSLIELKPASEYFSSEEAESVSA 189

Query: 189 AFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDPCHLPNNPG 248
           A   WR SDLT+D+PFFLV + S S+ASI+HL D E  + + +KLLFGFYDPCHLP+NPG
Sbjct: 190 ACNDWRDSDLTTDVPFFLVSVSSDSKASIRHLKDLEACQGDHQKLLFGFYDPCHLPSNPG 249

Query: 249 WPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQRCMPNAVG 308
           WPLRN+LALI SRWNL++V F CYRE+RGFADL LSLVG+A I    G      +PN+VG
Sbjct: 250 WPLRNYLALIRSRWNLETVWFFCYRESRGFADLNLSLVGQASITLSSG-ESAETVPNSVG 309

Query: 309 WELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSLKCLLLGAG 368
           WELN+GK+  RSI+LA SMDPTRLA++A DLNL+LMRWRALPSLNLN+LSS+KCLLLGAG
Sbjct: 310 WELNKGKRVPRSISLANSMDPTRLAVSAVDLNLKLMRWRALPSLNLNVLSSVKCLLLGAG 369

Query: 369 TLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKAAVKSLDRI 428
           TLGCQVAR LM WGIR IT VD G+VAMSNP+RQSLY  EDCL  G+FKA AAVKSL +I
Sbjct: 370 TLGCQVARTLMGWGIRNITFVDYGKVAMSNPVRQSLYNFEDCLGRGEFKAVAAVKSLKQI 429

Query: 429 FPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTRESRWLPTL 488
           FP ME  GVV++IPMPGHP+   E  S++ DC+RL +LI SHDAVFLLTDTRESRWLP+L
Sbjct: 430 FPAMETSGVVMAIPMPGHPISSQEEDSVLGDCKRLSELIESHDAVFLLTDTRESRWLPSL 489

Query: 489 LCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRRQRLGCYFC 548
           LCAN NK+ INAALGFDS++VMRHGAGP+    D      M NL +N T+  QRLGCYFC
Sbjct: 490 LCANANKIAINAALGFDSYMVMRHGAGPTSLSDD------MQNLDINKTN-TQRLGCYFC 549

Query: 549 NDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEGELLNSGID 608
           NDVVAP DS T+RTLDQQCTVTRPGLAPIA ++AVELLVG+L HP GI A+G+  NS + 
Sbjct: 550 NDVVAPQDSMTDRTLDQQCTVTRPGLAPIAGALAVELLVGVLQHPLGINAKGD--NSSLS 609

Query: 609 GT---SEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDFILEAI 668
            T    + PLGILPHQIRGS+SQFSQ+TL+G++SNSCTACS TV+SEYR RG  FILEAI
Sbjct: 610 NTGNNDDSPLGILPHQIRGSVSQFSQITLLGQASNSCTACSETVISEYRERGNSFILEAI 669

Query: 669 NHPTYLEDLTGLTELMKSTSSFQLDW-DNDTDGSD 700
           NHPTYLEDLTGLTEL K+ +SF LDW D+DTD  D
Sbjct: 670 NHPTYLEDLTGLTELKKAANSFNLDWEDDDTDDDD 692

BLAST of Moc01g32420 vs. ExPASy Swiss-Prot
Match: Q5ZKY2 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Gallus gallus OX=9031 GN=ATG7 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 7.9e-161
Identity = 326/709 (45.98%), Postives = 444/709 (62.62%), Query Frame = 0

Query: 8   SILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHMTLLSESL 67
           S LQFAPF S+++ GFWH L+  KLNE  +D++P  I G+Y     S     +TL   + 
Sbjct: 17  SKLQFAPFSSALNVGFWHELTQKKLNEYRLDETPKVIKGYYYNGDPSGFPARLTLEYSAF 76

Query: 68  PSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIREDILSGK 127
              A    S P+        C   G LYNTNT E+F + DK+SLL+ EA +I E I SG 
Sbjct: 77  DINA----SIPARC------CPAFGTLYNTNTFETFKSCDKKSLLEKEANEIWESIKSGA 136

Query: 128 AIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPA-SHWLNSKEAESV 187
           A+E+  +L RFLL++FADLKK++FYYWF +PAL       V++         +  + +++
Sbjct: 137 ALENPMLLNRFLLLTFADLKKYHFYYWFCYPALCFPDGIHVIQKPVCLGDRFSLNQIQAL 196

Query: 188 SSAFGQWRSSDLTSDIPFFLV--HIDSSSQASIKHLTDFETFESNGEKLLFGFYDPCHLP 247
             A+ +   ++  +  P+FL+  H +S   + +K    F  F+  G K+  G YDPC+L 
Sbjct: 197 QKAYDELCQTEGVTAFPYFLIKYHDNSVVVSPLKKWDGF--FQDQGGKVTVGVYDPCNLS 256

Query: 248 NNPGWPLRNFLALIYSRWN--LKSVNFLCYREN--RGFADLTLSLVGKALIDAPQGLIDQ 307
           + PGWPLRNFL L   +W   L+S+  LC+R+   +G  D+T S++ +  I  PQG    
Sbjct: 257 HYPGWPLRNFLILASHKWGNILQSIEVLCFRDRTMQGVRDITHSIIFE--IKLPQGAFGP 316

Query: 308 RCMPNAVGWELN-RGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSS 367
            C P AVGWE N +G    R +NL++ MDP RLA ++ DLNL+LM WR +P+L+L  + S
Sbjct: 317 DC-PKAVGWEKNQKGGMGPRVVNLSECMDPKRLAESSVDLNLKLMCWRLVPTLDLEKIVS 376

Query: 368 LKCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAK 427
            KCLLLGAGTLGC VAR LM WG+RKIT VD+ R++ SNP+RQ LY  EDCL+GG  KA 
Sbjct: 377 AKCLLLGAGTLGCSVARTLMGWGVRKITFVDNARISYSNPVRQPLYEFEDCLSGGKPKAL 436

Query: 428 AAVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEV--ASMIDDCRRLDDLINSHDAVFLLT 487
           AA + L +IFPG+ +EG  +SIPMPGHPV   EV  A    D   L++LI++HD VFLL 
Sbjct: 437 AAAERLQKIFPGVNSEGYNMSIPMPGHPVNFSEVTMAQARKDVATLEELIDAHDVVFLLM 496

Query: 488 DTRESRWLPTLLCANTNKVTINAALGFDSFLVMRHG------AGPSGSCHD-ATSQINMA 547
           DTRESRWLP ++ A+  K+ INAALGFD+F+VMRHG           +C   A    ++ 
Sbjct: 497 DTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQETGNACFSTAPGPSDLL 556

Query: 548 NLSLNATDRRQRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGIL 607
             SL +     +LGCYFCNDVVAP DST +RTLDQQCTV+RPGLA IA ++AVEL+V +L
Sbjct: 557 GSSLFSNIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAMIAGALAVELMVSVL 616

Query: 608 HHPEGIYAEGELLNSGIDGTSEQP--LGILPHQIRGSLSQFSQMTLVGRSSNSCTACSST 667
            HPEG YA   + +S  D  +E P  LG++PHQIRG LS+F  +  V  + + CTACS  
Sbjct: 617 QHPEGGYA---VASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSPK 676

Query: 668 VVSEYRNRGMDFILEAINHP-TYLEDLTGLTELMKSTSSFQLDWDNDTD 697
           V+ +Y   G +F+ +  N   ++LEDLTGLT L + T + ++ WD   D
Sbjct: 677 VLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEI-WDMSDD 706

BLAST of Moc01g32420 vs. ExPASy Swiss-Prot
Match: O95352 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Homo sapiens OX=9606 GN=ATG7 PE=1 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 8.7e-160
Identity = 326/717 (45.47%), Postives = 443/717 (61.79%), Query Frame = 0

Query: 2   AANDKG-SILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 61
           A  D G S LQFAPF S++D GFWH L+  KLNE  +D++P  I G+Y            
Sbjct: 4   ATGDPGLSKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGD-------- 63

Query: 62  TLLSESLPSEASRQPSAPSTSTGNRNR-CSVPGILYNTNTVESFHALDKQSLLKSEAKKI 121
              S  LP+  + + SA   S     R C   G LYNTNT+ESF   DK+ LL+  A +I
Sbjct: 64  ---SAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTADKKLLLEQAANEI 123

Query: 122 REDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPA-SHWL 181
            E I SG A+E+  +L +FLL++FADLKK++FYYWF +PAL L     +++         
Sbjct: 124 WESIKSGTALENPVLLNKFLLLTFADLKKYHFYYWFCYPALCLPESLPLIQGPVGLDQRF 183

Query: 182 NSKEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSS--QASIKHLTDFETFESNGEKLLFG 241
           + K+ E++  A+     ++  + +P+FL+  D +    + +KH +DF  F+    K+  G
Sbjct: 184 SLKQIEALECAYDNLCQTEGVTALPYFLIKYDENMVLVSLLKHYSDF--FQGQRTKITIG 243

Query: 242 FYDPCHLPNNPGWPLRNFLALIYSRW--NLKSVNFLCYREN--RGFADLTLSLVGKALID 301
            YDPC+L   PGWPLRNFL L   RW  + +SV  +C+R+   +G  D+  S++ +  + 
Sbjct: 244 VYDPCNLAQYPGWPLRNFLVLAAHRWSSSFQSVEVVCFRDRTMQGARDVAHSIIFE--VK 303

Query: 302 APQGLIDQRCMPNAVGWELN-RGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPS 361
            P+      C P AVGWE N +G    R +NL++ MDP RLA ++ DLNL+LM WR +P+
Sbjct: 304 LPEMAFSPDC-PKAVGWEKNQKGGMGPRMVNLSECMDPKRLAESSVDLNLKLMCWRLVPT 363

Query: 362 LNLNILSSLKCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCL 421
           L+L+ + S+KCLLLGAGTLGC VAR LM WG+R IT VD+ +++ SNP+RQ LY  EDCL
Sbjct: 364 LDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCL 423

Query: 422 NGGDFKAKAAVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVA--SMIDDCRRLDDLINS 481
            GG  KA AA   L +IFPG+ A G  +SIPMPGHPV    V       D  +L+ LI S
Sbjct: 424 GGGKPKALAAADRLQKIFPGVNARGFNMSIPMPGHPVNFSSVTLEQARRDVEQLEQLIES 483

Query: 482 HDAVFLLTDTRESRWLPTLLCANTNKVTINAALGFDSFLVMRHG------AGPSGSC-HD 541
           HD VFLL DTRESRWLP ++ A+  K+ INAALGFD+F+VMRHG       G    C + 
Sbjct: 484 HDVVFLLMDTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPNH 543

Query: 542 ATSQINMANLSLNATDRRQRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIA 601
             +  ++   SL A     +LGCYFCNDVVAP DST +RTLDQQCTV+RPGLA IA ++A
Sbjct: 544 PVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALA 603

Query: 602 VELLVGILHHPEGIYAEGELLNSGIDGTSEQP--LGILPHQIRGSLSQFSQMTLVGRSSN 661
           VEL+V +L HPEG YA   + +S  D  +E P  LG++PHQIRG LS+F  +  V  + +
Sbjct: 604 VELMVSVLQHPEGGYA---IASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFD 663

Query: 662 SCTACSSTVVSEYRNRGMDFILEAINHP-TYLEDLTGLTELMKSTSSFQLDWDNDTD 697
            CTACSS V+ +Y   G +F+ +  N   ++LEDLTGLT L + T + ++ WD   D
Sbjct: 664 KCTACSSKVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEI-WDMSDD 700

BLAST of Moc01g32420 vs. ExPASy Swiss-Prot
Match: Q641Y5 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Rattus norvegicus OX=10116 GN=Atg7 PE=2 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 2.5e-159
Identity = 320/704 (45.45%), Postives = 440/704 (62.50%), Query Frame = 0

Query: 8   SILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHMTLLSESL 67
           S LQFAPF S++D GFWH L+  KLNE  +D++P  I G+Y     + +   +TL     
Sbjct: 7   SKLQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTL----- 66

Query: 68  PSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIREDILSGK 127
              ++   SAP+ +      C   G L+NTNT+E+F   DK+ LL+  A +I E I SG 
Sbjct: 67  -EFSAFDMSAPTPA----RCCPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGA 126

Query: 128 AIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPA-SHWLNSKEAESV 187
           A+E+  +L +FLL++FADLKK++FYYWF  PAL L     ++         L+ K+ +++
Sbjct: 127 ALENPMLLNKFLLLTFADLKKYHFYYWFCCPALCLPESIPLIRGPVGLDQRLSPKQIQAL 186

Query: 188 SSAFGQWRSSDLTSDIPFFLVHIDSSS--QASIKHLTDFETFESNGEKLLFGFYDPCHLP 247
             A+     ++  + +P+FL   D  +   + +KH +DF  F+    KL  G YDPC+L 
Sbjct: 187 EHAYDDLCRTEGVTALPYFLFKYDDDTVLVSLLKHYSDF--FQGQRTKLTVGVYDPCNLT 246

Query: 248 NNPGWPLRNFLALIYSRW--NLKSVNFLCYREN--RGFADLTLSLVGKALIDAPQGLIDQ 307
            +PGWPLRNFL L   RW  + +SV  LC+R+   +G  D+T S++ +  +  P+     
Sbjct: 247 QHPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSIIFE--VKLPEMAFSP 306

Query: 308 RCMPNAVGWELN-RGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSS 367
            C P AVGWE N +G    R +NL+  MDP RLA ++ DLNL+LM WR +P+L+L+ + S
Sbjct: 307 DC-PKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVS 366

Query: 368 LKCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAK 427
           +KCLLLGAGTLGC VAR LM WG+R +T VD+ +++ SNP+RQ LY  EDCL GG  KA 
Sbjct: 367 VKCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKAL 426

Query: 428 AAVKSLDRIFPGMEAEGVVISIPMPGHPV--PDHEVASMIDDCRRLDDLINSHDAVFLLT 487
           AA + L +IFPG+ A G  +SIPMPGHPV   D  +     D  +L++LI+SHD +FLL 
Sbjct: 427 AAAERLQKIFPGVNASGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEELIDSHDVIFLLM 486

Query: 488 DTRESRWLPTLLCANTNKVTINAALGFDSFLVMRHG------AGPSGSCHDATSQINMAN 547
           DTRESRWLPT++ A+  K+ INAALGFD+F+VMRHG       G    C           
Sbjct: 487 DTRESRWLPTVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLG 546

Query: 548 LSLNATDRRQRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILH 607
            SL A     +LGCYFCNDVVAP DST +RTLDQQCTV+RPGLA IA ++AVEL+V +L 
Sbjct: 547 SSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQ 606

Query: 608 HPEGIYAEGELLNSGIDGTSEQP--LGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTV 667
           HPEG YA   + +S  D  +E P  LG++PHQIRG LS+F  +  V  + + CTACSS V
Sbjct: 607 HPEGGYA---IASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKV 666

Query: 668 VSEYRNRGMDFILEAINHP-TYLEDLTGLTELMKSTSSFQLDWD 693
           + +Y   G  F+ +  N   ++LEDLTGLT L + T + ++ WD
Sbjct: 667 LDQYEQEGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEI-WD 691

BLAST of Moc01g32420 vs. ExPASy Swiss-Prot
Match: Q9D906 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Mus musculus OX=10090 GN=Atg7 PE=1 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 2.8e-158
Identity = 318/703 (45.23%), Postives = 435/703 (61.88%), Query Frame = 0

Query: 10  LQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHMTLLSESLPS 69
           LQFAPF S++D GFWH L+  KLNE  +D++P  I G+Y     + +   +TL   +   
Sbjct: 9   LQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTLEFSAFDM 68

Query: 70  EASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIREDILSGKAI 129
            AS               C   G L+NTNT+E+F   DK+ LL+  A +I E I SG A+
Sbjct: 69  SASTPAHC----------CPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGAAL 128

Query: 130 EDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPAS--HWLNSKEAESVS 189
           E+  +L +FLL++FADLKK++FYYWF  PAL L P +  +   P S    L+ K+ +++ 
Sbjct: 129 ENPMLLNKFLLLTFADLKKYHFYYWFCCPALCL-PESIPLIRGPVSLDQRLSPKQIQALE 188

Query: 190 SAFGQWRSSDLTSDIPFFLVHIDSSS--QASIKHLTDFETFESNGEKLLFGFYDPCHLPN 249
            A+     ++  + +P+FL   D  +   + +KH +DF  F+    K+  G YDPC+L  
Sbjct: 189 HAYDDLCRAEGVTALPYFLFKYDDDTVLVSLLKHYSDF--FQGQRTKITVGVYDPCNLAQ 248

Query: 250 NPGWPLRNFLALIYSRW--NLKSVNFLCYREN--RGFADLTLSLVGKALIDAPQGLIDQR 309
            PGWPLRNFL L   RW  + +SV  LC+R+   +G  D+T S++ +  +  P+      
Sbjct: 249 YPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSIIFE--VKLPEMAFSPD 308

Query: 310 CMPNAVGWELN-RGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 369
           C P AVGWE N +G    R +NL+  MDP RLA ++ DLNL+LM WR +P+L+L+ + S+
Sbjct: 309 C-PKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSV 368

Query: 370 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 429
           KCLLLGAGTLGC VAR LM WG+R +T VD+ +++ SNP+RQ LY  EDCL GG  KA A
Sbjct: 369 KCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALA 428

Query: 430 AVKSLDRIFPGMEAEGVVISIPMPGHPV--PDHEVASMIDDCRRLDDLINSHDAVFLLTD 489
           A + L +IFPG+ A G  +SIPMPGHPV   D  +     D  +L+ LI++HD +FLL D
Sbjct: 429 AAERLQKIFPGVNARGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEQLIDNHDVIFLLMD 488

Query: 490 TRESRWLPTLLCANTNKVTINAALGFDSFLVMRHG------AGPSGSCHDATSQINMANL 549
           TRESRWLPT++ A+  K+ INAALGFD+F+VMRHG       G    C            
Sbjct: 489 TRESRWLPTVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLGS 548

Query: 550 SLNATDRRQRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHH 609
           SL A     +LGCYFCNDVVAP DST +RTLDQQCTV+RPGLA IA ++AVEL+V +L H
Sbjct: 549 SLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQH 608

Query: 610 PEGIYAEGELLNSGIDGTSEQP--LGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVV 669
           PEG YA   + +S  D  +E P  LG++PHQIRG LS+F  +  V  + + CTACS  V+
Sbjct: 609 PEGGYA---IASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSPKVL 668

Query: 670 SEYRNRGMDFILEAINHP-TYLEDLTGLTELMKSTSSFQLDWD 693
            +Y   G  F+ +  N   ++LEDLTGLT L + T + ++ WD
Sbjct: 669 DQYEREGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEI-WD 691

BLAST of Moc01g32420 vs. ExPASy TrEMBL
Match: A0A6J1CLS4 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Momordica charantia OX=3673 GN=LOC111012450 PE=3 SV=1)

HSP 1 Score: 1425.2 bits (3688), Expect = 0.0e+00
Identity = 708/708 (100.00%), Postives = 708/708 (100.00%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60
           MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM
Sbjct: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60

Query: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120
           TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR
Sbjct: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120

Query: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180
           EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS
Sbjct: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180

Query: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240
           KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP
Sbjct: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240

Query: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300
           CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ
Sbjct: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300

Query: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360
           RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL
Sbjct: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360

Query: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420
           KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA
Sbjct: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420

Query: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480
           AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR
Sbjct: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480

Query: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540
           ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR
Sbjct: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540

Query: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600
           QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG
Sbjct: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600

Query: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660
           ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF
Sbjct: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660

Query: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 709
           ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI
Sbjct: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 708

BLAST of Moc01g32420 vs. ExPASy TrEMBL
Match: A0A1S3AZ28 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis melo OX=3656 GN=LOC103484451 PE=3 SV=1)

HSP 1 Score: 1295.0 bits (3350), Expect = 0.0e+00
Identity = 640/708 (90.40%), Postives = 674/708 (95.20%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60
           MAANDKGSILQFAPFQS+VDEGFWHRLSSLKLN+LGIDDSPIPITGF+APCSHSQVSNH+
Sbjct: 1   MAANDKGSILQFAPFQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSQVSNHL 60

Query: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120
           TLLSESLP+E  R  S P T+ GNRNRC+VPGILYNTNTVESFHAL+K SLLKSEAKKI 
Sbjct: 61  TLLSESLPTEVKRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALEKLSLLKSEAKKIW 120

Query: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180
           EDILSGKA+EDSSVLARFLLISFADLKKWNF+YWFAFPALVLDPPATVVEL+ AS W N 
Sbjct: 121 EDILSGKALEDSSVLARFLLISFADLKKWNFHYWFAFPALVLDPPATVVELKSASQWFNL 180

Query: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240
           +EAES+S+AFGQWRSSDLTSDIPFFLV IDS+SQASIKHL DFET ++NGEKLLFGFYDP
Sbjct: 181 EEAESLSTAFGQWRSSDLTSDIPFFLVIIDSNSQASIKHLRDFETCQNNGEKLLFGFYDP 240

Query: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300
           CHLP+NPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALID P+GL DQ
Sbjct: 241 CHLPSNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDPKGLRDQ 300

Query: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360
            CMPNAVGWELN+GKK  ++INLAKSMDPTRLAI+AADLNLRLMRWRALPSLN+NILSSL
Sbjct: 301 CCMPNAVGWELNKGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSL 360

Query: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420
           KCLLLGAGTLGCQVARMLMAWG+RKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKA+A
Sbjct: 361 KCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAEA 420

Query: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480
           AVKSL+RIFP MEAEGVVISIPMPGHPVPDHE AS IDDCRRLDDLINSHDAVFLLTDTR
Sbjct: 421 AVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAVFLLTDTR 480

Query: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540
           ESRWLPTLLCAN NKVTI AALGFDSFLVMRHGAGPSGSCHD+TSQ N+ANLSLNAT+ R
Sbjct: 481 ESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNATNTR 540

Query: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600
           QRLGCYFCNDVVAPIDST NRTLDQQCTVTRPGLAPIAS+IAVELLVGILHHPEGIYAEG
Sbjct: 541 QRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEG 600

Query: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660
           ELLNSGIDG SEQPLGILPHQIRG LSQFSQMTLVGRSSNSCTACSS VVSEYRNRGMDF
Sbjct: 601 ELLNSGIDGASEQPLGILPHQIRGFLSQFSQMTLVGRSSNSCTACSSMVVSEYRNRGMDF 660

Query: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 709
           IL+AINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGS +D DGC+EI
Sbjct: 661 ILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSGDD-DGCIEI 707

BLAST of Moc01g32420 vs. ExPASy TrEMBL
Match: A0A6J1EF00 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucurbita moschata OX=3662 GN=LOC111433608 PE=3 SV=1)

HSP 1 Score: 1282.7 bits (3318), Expect = 0.0e+00
Identity = 637/708 (89.97%), Postives = 670/708 (94.63%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60
           MAANDKGSILQFAPFQS VDEGFWHRLSSLKLNELGIDDSPIPITGF+APC+HSQVSNH+
Sbjct: 1   MAANDKGSILQFAPFQSLVDEGFWHRLSSLKLNELGIDDSPIPITGFFAPCAHSQVSNHL 60

Query: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120
           TLLSESLPSE S + SAP T+ GNRNRCSVPGILYNTNTVESFHALDK SLLKSEAKKI 
Sbjct: 61  TLLSESLPSEVSSESSAPLTTKGNRNRCSVPGILYNTNTVESFHALDKLSLLKSEAKKIW 120

Query: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180
           EDILSGKAIEDSSVLARFLLISFADLKKWNF+YWFAFPALVL+PPATVVEL+PASHW N 
Sbjct: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFHYWFAFPALVLNPPATVVELKPASHWFNL 180

Query: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240
           +EAESVSS+F QWRSS+LTSDIPFFLV+IDSSSQASIKHL DFE  ++NGEKLLFGFYDP
Sbjct: 181 EEAESVSSSFAQWRSSNLTSDIPFFLVNIDSSSQASIKHLRDFEACQNNGEKLLFGFYDP 240

Query: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300
           CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALID  QG  DQ
Sbjct: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDTQGFRDQ 300

Query: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360
           +CMPN VGWELNRGKK  +SINLAKSMDPTRLAI+AADLNLRLMRWRALPSLN+NILSSL
Sbjct: 301 QCMPNVVGWELNRGKKCHKSINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSL 360

Query: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420
           KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYT+EDCLNGGDFKA+A
Sbjct: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTMEDCLNGGDFKAEA 420

Query: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480
           AVKSL+RIFP MEAEGVVISIPMPGHPV D+E ASM+DDCRRLDDLINSHDAVFLLTDTR
Sbjct: 421 AVKSLNRIFPAMEAEGVVISIPMPGHPVSDNEAASMLDDCRRLDDLINSHDAVFLLTDTR 480

Query: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540
           ESRWLPTLLCANTNKVTI AALGFDSFLVMRHGAGPSGSCHD+TSQ N+ANLSLNA + +
Sbjct: 481 ESRWLPTLLCANTNKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNAANTK 540

Query: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600
           QRLGCYFCNDVVAPIDST NRTLDQQCTVTRPGLAPIAS+IAVELLVGILHHPEGIYAE 
Sbjct: 541 QRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEC 600

Query: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660
           EL NS   G SEQPLGILPHQIRGSLSQFSQMTLVGRSSN+CTACSS VVSEYRNRGMDF
Sbjct: 601 ELSNS---GASEQPLGILPHQIRGSLSQFSQMTLVGRSSNTCTACSSLVVSEYRNRGMDF 660

Query: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 709
           IL+AINHPTYLEDLTGLTELMKSTSSFQLDWDN TDGS +D+DGC+EI
Sbjct: 661 ILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNYTDGSGDDDDGCIEI 705

BLAST of Moc01g32420 vs. ExPASy TrEMBL
Match: A0A6J1KW24 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucurbita maxima OX=3661 GN=LOC111496942 PE=3 SV=1)

HSP 1 Score: 1275.8 bits (3300), Expect = 0.0e+00
Identity = 636/709 (89.70%), Postives = 670/709 (94.50%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHM 60
           MAANDKGSILQFAPFQS VDEGFWHRLSSLKLNELGIDDSPIPITGF+APC+HSQVSNH+
Sbjct: 1   MAANDKGSILQFAPFQSLVDEGFWHRLSSLKLNELGIDDSPIPITGFFAPCAHSQVSNHL 60

Query: 61  TLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIR 120
           TLLSESL SE S + SAP T+ GNRNRCSVPGILYNTNTVESFHALDK SLLKSEAKKI 
Sbjct: 61  TLLSESLASEVSSESSAPLTTKGNRNRCSVPGILYNTNTVESFHALDKLSLLKSEAKKIW 120

Query: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNS 180
           EDILSGKAIEDSSVLARFLLISFADLKKWNF+YWFAFPALVL+PPATVVEL+PASHW N 
Sbjct: 121 EDILSGKAIEDSSVLARFLLISFADLKKWNFHYWFAFPALVLNPPATVVELKPASHWFNL 180

Query: 181 KEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDP 240
           +EAESVSS+F QWRSS+LTSD PFFLV+IDSSSQASIKHL DFE  ++NGEKLLFGFYDP
Sbjct: 181 EEAESVSSSFAQWRSSNLTSDTPFFLVNIDSSSQASIKHLRDFEACKNNGEKLLFGFYDP 240

Query: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQ 300
           CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALID  QG  DQ
Sbjct: 241 CHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDTQGFRDQ 300

Query: 301 RCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSL 360
           +CMPN VGWELNRGKK  +SINLAKSMDPTRLAI+AADLNLRLMRWRALPSLN+NILSSL
Sbjct: 301 QCMPNVVGWELNRGKKCHKSINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSL 360

Query: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKA 420
           KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYT+EDCLNGG+FKA+A
Sbjct: 361 KCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTMEDCLNGGEFKAEA 420

Query: 421 AVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTR 480
           AVKSL+RIFP MEAEGVVISIPMPGHPV D+E ASM+DDCRRLDDLINSHDAVFLLTDTR
Sbjct: 421 AVKSLNRIFPAMEAEGVVISIPMPGHPVSDNEAASMLDDCRRLDDLINSHDAVFLLTDTR 480

Query: 481 ESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRR 540
           ESRWLPTLLCANTNKVTI AALGFDSFLVMRHGAGPSGSCHD+TSQ N+ANLSLNAT+ +
Sbjct: 481 ESRWLPTLLCANTNKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNATNTK 540

Query: 541 QRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEG 600
           QRLGCYFCNDVVAPIDST NRTLDQQCTVTRPGLAPIAS+IAVELLVGILHHPEGIYAE 
Sbjct: 541 QRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEC 600

Query: 601 ELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDF 660
           EL NS   G SEQPLGILPHQIRGSLSQFSQMTLVGRSSN+CTACSS VVSEYRNRGMDF
Sbjct: 601 ELSNS---GASEQPLGILPHQIRGSLSQFSQMTLVGRSSNTCTACSSLVVSEYRNRGMDF 660

Query: 661 ILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGS-DEDNDGCVEI 709
           IL+AINHPTYLEDLTGLTELMKSTSSFQLDWDN TDGS D+D+DGC+EI
Sbjct: 661 ILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNYTDGSGDDDDDGCIEI 706

BLAST of Moc01g32420 vs. ExPASy TrEMBL
Match: A0A5D3CNQ1 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008180 PE=3 SV=1)

HSP 1 Score: 1249.6 bits (3232), Expect = 0.0e+00
Identity = 626/711 (88.05%), Postives = 659/711 (92.69%), Query Frame = 0

Query: 1   MAANDKGSILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITG---FYAPCSHSQVS 60
           MAANDKGSILQFAPFQS+VDEGFWHRLSSLKLN+LGIDDSPIPITG   F+ P S     
Sbjct: 1   MAANDKGSILQFAPFQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGLFLFFFPFS----- 60

Query: 61  NHMTLLSESLPSEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAK 120
                   SLP+E  R  S P T+ GNRNRC+VPGILYNTNTVESFHAL+K SLLKSEAK
Sbjct: 61  -----FFYSLPTEVKRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALEKLSLLKSEAK 120

Query: 121 KIREDILSGKAIEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHW 180
           KI EDILSGKA+EDSSVLARFLLISFADLKKWNF+YWFAFPALVLDPPATVVEL+ AS W
Sbjct: 121 KIWEDILSGKALEDSSVLARFLLISFADLKKWNFHYWFAFPALVLDPPATVVELKSASQW 180

Query: 181 LNSKEAESVSSAFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGF 240
            N +EAES+S+AFGQWRSSDLTSDIPFFLV IDS+SQASIKHL DFET ++NGEKLLFGF
Sbjct: 181 FNLEEAESLSTAFGQWRSSDLTSDIPFFLVIIDSNSQASIKHLRDFETCQNNGEKLLFGF 240

Query: 241 YDPCHLPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGL 300
           YDPCHLP+NPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALID P+GL
Sbjct: 241 YDPCHLPSNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDPKGL 300

Query: 301 IDQRCMPNAVGWELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNIL 360
            DQ CMPNAVGWELN+GKK  ++INLAKSMDPTRLAI+AADLNLRLMRWRALPSLN+NIL
Sbjct: 301 RDQCCMPNAVGWELNKGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINIL 360

Query: 361 SSLKCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFK 420
           SSLKCLLLGAGTLGCQVARMLMAWG+RKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFK
Sbjct: 361 SSLKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFK 420

Query: 421 AKAAVKSLDRIFPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLT 480
           A+AAVKSL+RIFP MEAEGVVISIPMPGHPVPDHE AS IDDCRRLDDLINSHDAVFLLT
Sbjct: 421 AEAAVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAVFLLT 480

Query: 481 DTRESRWLPTLLCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNAT 540
           DTRESRWLPTLLCAN NKVTI AALGFDSFLVMRHGAGPSGSCHD+TSQ N+ANLSLNAT
Sbjct: 481 DTRESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNAT 540

Query: 541 DRRQRLGCYFCNDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIY 600
           + RQRLGCYFCNDVVAPIDST NRTLDQQCTVTRPGLAPIAS+IAVELLVGILHHPEGIY
Sbjct: 541 NTRQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIY 600

Query: 601 AEGELLNSGIDGTSEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRG 660
           AEGELLNSGIDG SEQPLGILPHQIRG LSQFSQMTLVGRSSNSCTACSS VVSEYRNRG
Sbjct: 601 AEGELLNSGIDGASEQPLGILPHQIRGFLSQFSQMTLVGRSSNSCTACSSMVVSEYRNRG 660

Query: 661 MDFILEAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSDEDNDGCVEI 709
           MDFIL+AINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGS +D DGC+EI
Sbjct: 661 MDFILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSGDD-DGCIEI 700

BLAST of Moc01g32420 vs. TAIR 10
Match: AT5G45900.1 (ThiF family protein )

HSP 1 Score: 981.1 bits (2535), Expect = 4.7e-286
Identity = 492/695 (70.79%), Postives = 569/695 (81.87%), Query Frame = 0

Query: 9   ILQFAPFQSSVDEGFWHRLSSLKLNELGIDDSPIPITGFYAPCSHSQVSNHMTLLSESLP 68
           ILQFAP  SSVDEGFWH  SSLKL++LGIDDSPI ITGFY PC H QVSNH+TLLSESLP
Sbjct: 10  ILQFAPLNSSVDEGFWHSFSSLKLDKLGIDDSPISITGFYGPCGHPQVSNHLTLLSESLP 69

Query: 69  SEASRQPSAPSTSTGNRNRCSVPGILYNTNTVESFHALDKQSLLKSEAKKIREDILSGKA 128
            +   Q    STS GNRN+C VPGILYNTNTVESF+ LDKQSLLK+EA KI EDI SGKA
Sbjct: 70  LD--EQSLIASTSHGNRNKCPVPGILYNTNTVESFNKLDKQSLLKAEANKIWEDIQSGKA 129

Query: 129 IEDSSVLARFLLISFADLKKWNFYYWFAFPALVLDPPATVVELQPASHWLNSKEAESVSS 188
           +ED SVL RFL+ISFADLKKW+F YWFAFPA VLDPP +++EL+PAS + +S+EAESVS+
Sbjct: 130 LEDPSVLPRFLVISFADLKKWSFRYWFAFPAFVLDPPVSLIELKPASEYFSSEEAESVSA 189

Query: 189 AFGQWRSSDLTSDIPFFLVHIDSSSQASIKHLTDFETFESNGEKLLFGFYDPCHLPNNPG 248
           A   WR SDLT+D+PFFLV + S S+ASI+HL D E  + + +KLLFGFYDPCHLP+NPG
Sbjct: 190 ACNDWRDSDLTTDVPFFLVSVSSDSKASIRHLKDLEACQGDHQKLLFGFYDPCHLPSNPG 249

Query: 249 WPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDAPQGLIDQRCMPNAVG 308
           WPLRN+LALI SRWNL++V F CYRE+RGFADL LSLVG+A I    G      +PN+VG
Sbjct: 250 WPLRNYLALIRSRWNLETVWFFCYRESRGFADLNLSLVGQASITLSSG-ESAETVPNSVG 309

Query: 309 WELNRGKKSCRSINLAKSMDPTRLAITAADLNLRLMRWRALPSLNLNILSSLKCLLLGAG 368
           WELN+GK+  RSI+LA SMDPTRLA++A DLNL+LMRWRALPSLNLN+LSS+KCLLLGAG
Sbjct: 310 WELNKGKRVPRSISLANSMDPTRLAVSAVDLNLKLMRWRALPSLNLNVLSSVKCLLLGAG 369

Query: 369 TLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAKAAVKSLDRI 428
           TLGCQVAR LM WGIR IT VD G+VAMSNP+RQSLY  EDCL  G+FKA AAVKSL +I
Sbjct: 370 TLGCQVARTLMGWGIRNITFVDYGKVAMSNPVRQSLYNFEDCLGRGEFKAVAAVKSLKQI 429

Query: 429 FPGMEAEGVVISIPMPGHPVPDHEVASMIDDCRRLDDLINSHDAVFLLTDTRESRWLPTL 488
           FP ME  GVV++IPMPGHP+   E  S++ DC+RL +LI SHDAVFLLTDTRESRWLP+L
Sbjct: 430 FPAMETSGVVMAIPMPGHPISSQEEDSVLGDCKRLSELIESHDAVFLLTDTRESRWLPSL 489

Query: 489 LCANTNKVTINAALGFDSFLVMRHGAGPSGSCHDATSQINMANLSLNATDRRQRLGCYFC 548
           LCAN NK+ INAALGFDS++VMRHGAGP+    D      M NL +N T+  QRLGCYFC
Sbjct: 490 LCANANKIAINAALGFDSYMVMRHGAGPTSLSDD------MQNLDINKTN-TQRLGCYFC 549

Query: 549 NDVVAPIDSTTNRTLDQQCTVTRPGLAPIASSIAVELLVGILHHPEGIYAEGELLNSGID 608
           NDVVAP DS T+RTLDQQCTVTRPGLAPIA ++AVELLVG+L HP GI A+G+  NS + 
Sbjct: 550 NDVVAPQDSMTDRTLDQQCTVTRPGLAPIAGALAVELLVGVLQHPLGINAKGD--NSSLS 609

Query: 609 GT---SEQPLGILPHQIRGSLSQFSQMTLVGRSSNSCTACSSTVVSEYRNRGMDFILEAI 668
            T    + PLGILPHQIRGS+SQFSQ+TL+G++SNSCTACS TV+SEYR RG  FILEAI
Sbjct: 610 NTGNNDDSPLGILPHQIRGSVSQFSQITLLGQASNSCTACSETVISEYRERGNSFILEAI 669

Query: 669 NHPTYLEDLTGLTELMKSTSSFQLDW-DNDTDGSD 700
           NHPTYLEDLTGLTEL K+ +SF LDW D+DTD  D
Sbjct: 670 NHPTYLEDLTGLTELKKAANSFNLDWEDDDTDDDD 692

BLAST of Moc01g32420 vs. TAIR 10
Match: AT5G19180.1 (E1 C-terminal related 1 )

HSP 1 Score: 48.9 bits (115), Expect = 1.9e-05
Identity = 28/84 (33.33%), Postives = 47/84 (55.95%), Query Frame = 0

Query: 350 PSLNLNILSSLKCLLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTLED 409
           P L  +I   ++ L++GAG LGC++ + L   G R + ++D  R+ ++N  RQ L+ +ED
Sbjct: 37  PGLRDDIRDYVRILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIED 96

Query: 410 CLNGGDFKAKAAVKSLDRIFPGME 434
               G  KA+ A K +     G+E
Sbjct: 97  V---GKPKAEVAAKRVMERVSGVE 117

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142296.10.0e+00100.00ubiquitin-like modifier-activating enzyme atg7 [Momordica charantia][more]
XP_008439746.10.0e+0090.40PREDICTED: ubiquitin-like modifier-activating enzyme atg7 [Cucumis melo][more]
XP_038880974.10.0e+0089.83ubiquitin-like modifier-activating enzyme atg7 [Benincasa hispida][more]
XP_022926466.10.0e+0089.97ubiquitin-like modifier-activating enzyme atg7 [Cucurbita moschata][more]
KAG6594550.10.0e+0089.83Ubiquitin-like modifier-activating enzyme atg7, partial [Cucurbita argyrosperma ... [more]
Match NameE-valueIdentityDescription
Q94CD56.6e-28570.79Ubiquitin-like modifier-activating enzyme atg7 OS=Arabidopsis thaliana OX=3702 G... [more]
Q5ZKY27.9e-16145.98Ubiquitin-like modifier-activating enzyme ATG7 OS=Gallus gallus OX=9031 GN=ATG7 ... [more]
O953528.7e-16045.47Ubiquitin-like modifier-activating enzyme ATG7 OS=Homo sapiens OX=9606 GN=ATG7 P... [more]
Q641Y52.5e-15945.45Ubiquitin-like modifier-activating enzyme ATG7 OS=Rattus norvegicus OX=10116 GN=... [more]
Q9D9062.8e-15845.23Ubiquitin-like modifier-activating enzyme ATG7 OS=Mus musculus OX=10090 GN=Atg7 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CLS40.0e+00100.00Ubiquitin-like modifier-activating enzyme ATG7 OS=Momordica charantia OX=3673 GN... [more]
A0A1S3AZ280.0e+0090.40Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1EF000.0e+0089.97Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KW240.0e+0089.70Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5D3CNQ10.0e+0088.05Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
AT5G45900.14.7e-28670.79ThiF family protein [more]
AT5G19180.11.9e-0533.33E1 C-terminal related 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042523Ubiquitin-like modifier-activating enzyme Atg7, N-terminal, subdomain 2GENE3D3.40.140.100coord: 10..293
e-value: 5.2E-124
score: 415.4
IPR032197Ubiquitin-like modifier-activating enzyme Atg7, N-terminalPFAMPF16420ATG7_Ncoord: 10..327
e-value: 4.2E-98
score: 328.8
IPR042522Ubiquitin-like modifier-activating enzyme Atg7, N-terminal, subdomain 1GENE3D3.40.140.70coord: 15..323
e-value: 5.2E-124
score: 415.4
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 355..643
e-value: 6.3E-40
score: 137.1
NoneNo IPR availableGENE3D3.40.50.720coord: 327..703
e-value: 3.8E-138
score: 461.8
NoneNo IPR availablePIRSRPIRSR606285-1PIRSR606285-1coord: 8..701
e-value: 1.5E-240
score: 798.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 688..708
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..88
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 693..708
NoneNo IPR availablePANTHERPTHR10953:SF3UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7coord: 10..694
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 10..694
NoneNo IPR availableCDDcd01486Apg7coord: 361..677
e-value: 0.0
score: 515.385
IPR006285Ubiquitin-like modifier-activating enzyme Atg7TIGRFAMTIGR01381TIGR01381coord: 12..700
e-value: 6.0E-282
score: 934.9
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 344..672

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g32420.1Moc01g32420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000045 autophagosome assembly
biological_process GO:0000422 autophagy of mitochondrion
biological_process GO:0006995 cellular response to nitrogen starvation
biological_process GO:0006501 C-terminal protein lipidation
biological_process GO:0050832 defense response to fungus
biological_process GO:0044805 late nucleophagy
biological_process GO:0010150 leaf senescence
biological_process GO:0034727 piecemeal microautophagy of the nucleus
biological_process GO:0032446 protein modification by small protein conjugation
biological_process GO:0015031 protein transport
cellular_component GO:0000407 phagophore assembly site
cellular_component GO:0005737 cytoplasm
molecular_function GO:0019778 Atg12 activating enzyme activity
molecular_function GO:0019779 Atg8 activating enzyme activity
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity