Moc01g26280 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g26280
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionNADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
Locationchr1: 18681208 .. 18686579 (+)
RNA-Seq ExpressionMoc01g26280
SyntenyMoc01g26280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAACTCTTTGCAGCGAATCTGGAACCACCGATGTGGTCTCCGGGGTACCCTGGCTCCTCTCTGCAGATCATTTTCCGCCGACTCCTTGGTTCAGGTCAAGCCCGGCGAGATCGGCATGGTCTCTGGAATTCCAGAGGAACACCTTACCAGGAGGGTATACCCCAGGCATCCATTTTCTGTACTATTTATCTATGAGTTTCTGATTGCTTACATTTTAGTGTATTTTCGAGGGATCGGGTTTATGGATGGTATTGATGATTTCTGGGGACTAGGTTTTTTTTTTTTTTTCCTACGCGTGTGGTTTTTATTTTTATTTTTATGAATGGTCTGCAGCGGCTAAACCCTTCAGGTGTTTATTTGTTGGTACTATTTCCAAATGTAGTCGGTTAACAAAAGGAGATGAAAGTTTTTTTTTTTTTTTTGTTCTGGCTAATTTATCTCTATGGCGTTGCTGTTATTACCAATTGTATGTTGATAGGCAAGGAAATTTATTAAAATAAGGTGAGAATTCTAGAGGAGATTAGAGGTACCGAGGTCCAATCACAGATTCACAAAGATCAGCTGCACCTTTTTTCTTTTGATTTTTTCGAAGCTACTCTATATTGTTACTGCTATTGGTTGCATTCACAAGATCCTAATCTATTGGTTGGCATTTAACAAAATATTTGATCAATTACCATCTTACTGTCTGTGTAAGTTATTCGGGACTAATCGTTGAATCATCCATACTTGACTAATAAAACGCGAATGTTGGGATTTTTTAATTTGCACTTAATCTTTTAGGGATTAACTTTACATGCTGAGGTTTAATCTTGAAGTTCTAAAATGAAAGCCATTTCCGTTGGGTTTGCTTGTAATATTCATCTTTTTTTTTTAATATGACCAGCTCGATTCATGTGTGTTCCTATTATGAAATTATACTAGGTTGTAATCTACTCTCCTGCTAGAACTGCATCTCAGCAAGGATCAGGCAAGGTTGGAAAGTGGAAAATCAATTTTATGTCAACGCAAAAGTATGAATTCTTATTTCCTTTATTTTTTTTCCTGGGTTTGGTTCTTTCAAAGATATGAAATTGTTGCTTCAGTAGTTAACTGAATTTTTAACTCAGTTCTAGCAGAGTGAAGAGTTTCCTTATGCTTTACCATCATGTTTGTAGGTGGGAAAACCCATTAATGGGTTGGACTTCTACTGGGGACCCCTACGCCAATGTAGGGGATTCTGCACTGTCTTTTGACAGCGAAGAAGCTGCAAAGGCATTTGCAGAGAAACATGGATGGGACTATGTGGTAATGCGTCGTTTAACCTTTTAGAATGAATGTTCTTAAAAGATTCCCTCTATGTAGTTTGAGTTTGTCATGTCTTAAATTAGTTATTAACTATGCTATTTTTTTACTCAGTTGCCATCAAATGAGTAATAATAGTATTTGGTGGTGTGGCTTGACGTGCATACATTCTTATATATTATTTGCGACAAATCTTTGCTATTCTACAAGGTTGGGATTTGAACCTCCGATCTCAAGGGAAAGAGTATATATCTTTATTGTTGAACTATGCTTACGTTGGCCTAATAAAATGATAAAATGAGATTGCCCTTGGATATGATGGTTTAATATGTATATCTAATACATATCATCAATGAAATGAAATTGATTTAAAATGTATGATTGAAATGAAATTGATACTAGGATATTTCATATCATCAATGAAATTGTACAAAAAATATGTATATCTAATACGATTCATGATTTTTGCATTCGAGCAATAATACGATATTCGGGATTTTTTACTTTGAATATAAGATAATGTTTTTGAAGATGGTGGAAAGAAATCCGATTATTTAATATCCTAGTATGATTTTTTTGTGAAAGAAACAATTTCATTGATATATAAGGAGCCCATATAAGGGAGTACAAAAGCCCATAGATGGGTTATAAAAGGCACTTCAAATTGGATAGGAGGGAAGTGTAGCTATAGTAAAGAAAAGTAGGAGAAGTAGCTATAGTAAAGAAAATTAGGAGAAGTTTTACACCATGATATAGCTTTAAACACTATAGCATCAAAAAAACGATCAAATGATTGTGACTTGTCAATGAAGATCCTTTGATTTCTTTCACCCCAAGTAGTCCACAGAAAGGCTCGAATGAAATTTGTCCATAGCTGTTCCTTTATGTTTTTGAAGGGGTGTCCCATTAAGGTGGCCTCAAGGAGAAGTTTAGGGTCATTTGGTAATGCTGTGGACCAACCAAAAGCCAAGAGGATGACATTCCAAAAATCAGAAGCAAAAGTACAGGAAGTGAAGAGATGTTGTTGAGATTCCATGCTGTTCTTGCAAATGGTACACCATTGTGGAGAAATAGCCATAAAAAGGAGTCTTCTTTGTAGCTTATCGTTGGTACTGATGGCTCGGTGACTAAGTTCCCATAGGAAGAAATTCACTTTTTTAGGGCAATTTCCATTCCGGATAATGTTGTATAGGCTAGTTTGTGGAGCTTGGTTGCTATGTGTGAGGTCCTTTGTGAGGGAGCTGGTGGAAAAAGAACCATTTGTTTCTAGTTTCCAGTAAACAACATCCCTATCTGCTGTTAAATGAATCTCTGATAGCCTAAGGTTAAGATTAGTCCATTCAATTATCTCATCATCTTTAAGGTTCCGCCTTAATCCCAAATTCCAGCAAGCTGTAGATTCGTCCCAGATATCAGAGATTGGGGCGTCCTTTCTTGTTGACAAAGCGAAAATGAGGGGAAATGATTGTTTAAAAGGCAAACTATTCATTCCATTTCCGGTTTTAAAGGCCAATCGGTCAAAAATCAAATTTTGCATCTTTCTAATGGCAGCCCATGGGCTTTTAGCAGAAGGTAGTGATGTAGAGTTAGGTTTGAGGTTCCATGATGCTTTACCAAATTTTGCCTCGATCACCTTCCTCCAAAGAGATGTTTCCTCTGTAATGAATCTCCATATCCATTTTGCAAGGAGGGTTCTGTTTTTCAGCTTCATATTAGCAATCCCTAGGCCTCCCATATGGATTTGATGTTGTATAGTGTCTCTTCTGGTGAGATGTAGTCCACTATTGTCTTTGTTACCCTTCCACAGAAATCTTGTGTAGATGTTGTCCAAAGTATGTGCTACTTTTGATGGCAAAGGGAAGAGGGAAAGATAGTAAGTAGGGAGGTTCGATAAAGTGGCTTGGATGAGAGTATGTCTACCGCCTTTGGAGAGATAGGAGGATTTCCATGATAGGAGACGTTTTTGAATTTTCTCAATCATTGGGTCCCAAAAAGAGAGTGACCGGTGCTTACCATGAAGGGGTAGTCCAAGGTATGTGGTCGGCCAAGTCCCCAAGTTACAAGCAAATTTGGATTGAAGAGCTGTCAGTTCATCCTCTCCAAGATTTATCCCAAAAATTTCTGACTTTTGGTGATTTATGTTTACCCCCGATGCCTCCTCAAAAAGCAAAATGATGTTTAACATGTTTGTGAGGGAGAGGTTATCATAAGGGGAGAAAAGGATGGTGTCATCAGCAAATTGTAGATGATGAATGGCCAATGAGTTGTTTCCCACGTCAAAACCTTAAGTTGATGTTTGCTGCATGTGTCATAAGTCTACTATAGACATCATTAATCATAATAAATAAAAAGGGAGAGAGGGGGTCACCTTGATGGAGGCCCCTAGAAGCAGTAAGTTTACCCCGAGGACGACCATTTATAATGATAGAAAAATTTGTTGTTGAGATACACCCCCAAATCCATTTTCTCCACGCGTGTCCGAAACCCTTGGCTGCAAGAACTTCATCAAGGAAGTCCCAATCCACTTTGTCAAAAGCCTTCTCAATATCAAGCGTGATAACCACTCATTTTCTCTTTTTTCTATCCCAATCCTCAATAATTTCGTTAGCAATTAAGGAGGCATCTAAGATTTGTCTTCCGGCCACAAATGCAGATTGCTCCTTGGTAATAGTACGTGGCAGTAAACTCTTCAACCTTTCTGAGAGAACTCTAGCTATAATCTTGTAGAGGCAAGAAATGAGGCTAATGGGCCGATAATCTCCTACCTTCCGAGCATCCACTTTCTTTGGGATGAGACAAATATAGGTTTCGTTGAGGCTAGCATTAATGATGCCGTTCTTAAAAAAATCTTGAAACACTCTCATAATGTCTGGCTTGAGAATGTTCCAAAATTGTTTAAAGAACTCGGCCGTGTAACCGTTAGGACCAGGAGACTTGTTTGATCCCAAAGGCTATTCGATATCTAAACAAAAGGCTGTTCGATATTTGGATGATTCCAGCTGAAGGTCAGCTTCTACATCTCACTATCATTATCATATCCTAGTGTGAATTTGAAGCCAAACTTCGGTAATTTAAAGAAGATAAATTTTGAGGGAAAGAAATGCCAGTTTTTCTTACAAATTTGGTCCATTAGAATTAGATATAAAAAGTTCATTTTTCTTATTTAGGTTTCTGTTGGCATTGTATTACAATTTTATCATTATCAGGAGAGGATCGTTAAAGGCATTTTCCCCCTACTTCTTTAATGTTAAGAATTACGTATAACTTTTCTGACCTGCAAATAATTATGAGATTACATGGAGAGGCAATCCCCAAACTTGGGATTAATTTCTAATTCATTCGCTGACTGTATTTCTTGATTTTGTTTTTCTTCTTTTTGGTAGGTTAAGAAGCGCCACACACCACTTTTGAAGGTTTGAGTTTCCAGTATTTTAACTTTCCTATGATTGAAATGATAATGAGATGTACATTTCCATTTCATTGAGCATGTACATTGTCTATAGTTCTGTGAATGCAAGCTTCGACTATGTTCAATGTCCTAAATGTTCCCTTGTGATAAATTTTATTCCTTTGATTTAATGCAACTTCTTTCATTATATTTTGGAGTTCTGCTGACATTTTATTTTCAATTTTAGTTGTTTCCTCCAATTTTTGTGGCCTTCCTGACATTTCTTATGGGATTTGTCTCCCTTTGCAATTTGTCTTCTTGTTGCTAGTTTAAGGGCTCAGCTTCAATTTCATTTTGATACCAATTAATTTTTTCCCAATTGACATTATTGAAGTTTGGGCTGTGACTATTGGAGTATTTGGATGTTGTAACAGCTAAGAGTTCCTTTAGATAAATTAGGAAGGAGGATGATTAAGATCTGACAAATTGGTTCTAAAATCAATCATGTTAGCATTCAATTGATGAAGGAATTTTAGTTCTGCTTGTTTTCTTACATATATTAAGTAAATGTTGGCTATTTAGTTCACTTGTGAGACGGAAAACAGGTAGGTTCTAACTTCCTGTTTTGACTGCAGGTGAAGGCATACGCTGACAATTTCAAATGGAAGGGCCCTCCTGAAGCCAAGGAGGCTTAA

mRNA sequence

ATGGCCAACTCTTTGCAGCGAATCTGGAACCACCGATGTGGTCTCCGGGGTACCCTGGCTCCTCTCTGCAGATCATTTTCCGCCGACTCCTTGGTTCAGGTCAAGCCCGGCGAGATCGGCATGGTCTCTGGAATTCCAGAGGAACACCTTACCAGGAGGGTTGTAATCTACTCTCCTGCTAGAACTGCATCTCAGCAAGGATCAGGCAAGGTTGGAAAGTGGAAAATCAATTTTATGTCAACGCAAAAGTGGGAAAACCCATTAATGGGTTGGACTTCTACTGGGGACCCCTACGCCAATGTAGGGGATTCTGCACTGTCTTTTGACAGCGAAGAAGCTGCAAAGGCATTTGCAGAGAAACATGGATGGGACTATGTGGTTAAGAAGCGCCACACACCACTTTTGAAGGTGAAGGCATACGCTGACAATTTCAAATGGAAGGGCCCTCCTGAAGCCAAGGAGGCTTAA

Coding sequence (CDS)

ATGGCCAACTCTTTGCAGCGAATCTGGAACCACCGATGTGGTCTCCGGGGTACCCTGGCTCCTCTCTGCAGATCATTTTCCGCCGACTCCTTGGTTCAGGTCAAGCCCGGCGAGATCGGCATGGTCTCTGGAATTCCAGAGGAACACCTTACCAGGAGGGTTGTAATCTACTCTCCTGCTAGAACTGCATCTCAGCAAGGATCAGGCAAGGTTGGAAAGTGGAAAATCAATTTTATGTCAACGCAAAAGTGGGAAAACCCATTAATGGGTTGGACTTCTACTGGGGACCCCTACGCCAATGTAGGGGATTCTGCACTGTCTTTTGACAGCGAAGAAGCTGCAAAGGCATTTGCAGAGAAACATGGATGGGACTATGTGGTTAAGAAGCGCCACACACCACTTTTGAAGGTGAAGGCATACGCTGACAATTTCAAATGGAAGGGCCCTCCTGAAGCCAAGGAGGCTTAA

Protein sequence

MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPARTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA
Homology
BLAST of Moc01g26280 vs. NCBI nr
Match: XP_022132610.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Momordica charantia])

HSP 1 Score: 327.4 bits (838), Expect = 6.7e-86
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA
Sbjct: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 155

BLAST of Moc01g26280 vs. NCBI nr
Match: XP_038881462.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 314.3 bits (804), Expect = 5.8e-82
Identity = 147/155 (94.84%), Postives = 152/155 (98.06%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW+H C LRGT+APLCRSFS DSLVQVKPGEIGMVSGIPEEHLTRRV+IYSPA
Sbjct: 1   MANSLQRIWSHGCALRGTVAPLCRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVIIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGW+YVV+KRHTPLLKVKAYADNFKWKGPPEAKEA
Sbjct: 121 HGWEYVVQKRHTPLLKVKAYADNFKWKGPPEAKEA 155

BLAST of Moc01g26280 vs. NCBI nr
Match: XP_022962977.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_022962978.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_023003205.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita maxima] >XP_023517797.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita pepo subsp. pepo] >KAG6594972.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 308.9 bits (790), Expect = 2.4e-80
Identity = 146/155 (94.19%), Postives = 149/155 (96.13%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWN  C LRGT+APLCRSFSADSLV+VKPGEIGMVSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGW+YVVKKRHTPLLK KAYADNFKWKG PE KEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHPEPKEA 155

BLAST of Moc01g26280 vs. NCBI nr
Match: XP_022977437.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 300.8 bits (769), Expect = 6.7e-78
Identity = 142/155 (91.61%), Postives = 150/155 (96.77%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW++   LRGT+APLCRSFS+DSLVQ+KPGEIGMVSGIP+EHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSNSRALRGTVAPLCRSFSSDSLVQLKPGEIGMVSGIPDEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAK FA+K
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKEFADK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGW+YVVKKRHTPLLKVKAYADNFKWKG PEAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGHPEAKEA 155

BLAST of Moc01g26280 vs. NCBI nr
Match: XP_022950018.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >KAG6604122.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 300.1 bits (767), Expect = 1.1e-77
Identity = 141/155 (90.97%), Postives = 150/155 (96.77%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW++   +RGT+APLCRSFS+DSLVQ+KPGEIGMVSGIP+EHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSNSRAIRGTVAPLCRSFSSDSLVQLKPGEIGMVSGIPDEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAK FA+K
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKEFADK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGW+YVVKKRHTPLLKVKAYADNFKWKG PEAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGHPEAKEA 155

BLAST of Moc01g26280 vs. ExPASy Swiss-Prot
Match: Q9FJW4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 3.0e-57
Identity = 103/139 (74.10%), Postives = 122/139 (87.77%), Query Frame = 0

Query: 15  LRGTLAPLCRSFSADSLVQ--VKPGEIGMVSGIPEEHLTRRVVIYSPARTASQQGSGKVG 74
           +  TL  + R F+ D++V+   K GEIG VSGIPEEHL+R+V+IYSPARTA+Q GSGK+G
Sbjct: 13  IAATLRRVARPFATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLG 72

Query: 75  KWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWDYVVKKRHT 134
           KWKINF+ST KWENPLMGWTSTGDPYANVGDSAL+FDSEEAAK+FAE+HGWDY VKK +T
Sbjct: 73  KWKINFVSTLKWENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNT 132

Query: 135 PLLKVKAYADNFKWKGPPE 152
           PLLKVK+Y+DNFKWKG P+
Sbjct: 133 PLLKVKSYSDNFKWKGNPQ 151

BLAST of Moc01g26280 vs. ExPASy Swiss-Prot
Match: Q5XIF3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ndufs4 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.0e-24
Identity = 53/110 (48.18%), Postives = 72/110 (65.45%), Query Frame = 0

Query: 38  EIGMVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGD 97
           ++  ++G+PEEH+ TR+V I+ PAR   Q G     KWK+ F + ++WENPLMGW ST D
Sbjct: 60  DVTPLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWDYVVKKRHTPLLKVKAYADNFKW 147
           P +N+    L+F ++E A AFAEKHGW Y V+ R  P  K K+Y  NF W
Sbjct: 120 PLSNM---VLTFSAKEDAVAFAEKHGWSYDVEGRKVPKPKSKSYGANFSW 166

BLAST of Moc01g26280 vs. ExPASy Swiss-Prot
Match: Q02375 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taurus OX=9913 GN=NDUFS4 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.8e-24
Identity = 52/110 (47.27%), Postives = 72/110 (65.45%), Query Frame = 0

Query: 38  EIGMVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGD 97
           +I  ++G+PEEH+ TR+  I+ PAR   Q G     KWK+ F + ++WENPLMGW ST D
Sbjct: 60  DITTITGVPEEHIKTRKARIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWDYVVKKRHTPLLKVKAYADNFKW 147
           P +N+    L+F ++E A AFAEK+GW Y V++R  P  K K+Y  NF W
Sbjct: 120 PLSNL---VLTFSTKEDAVAFAEKNGWSYDVEERKVPKPKSKSYGANFSW 166

BLAST of Moc01g26280 vs. ExPASy Swiss-Prot
Match: Q66XS7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gekko japonicus OX=146911 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 7.5e-24
Identity = 52/110 (47.27%), Postives = 74/110 (67.27%), Query Frame = 0

Query: 38  EIGMVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGD 97
           +I  ++G+PEEH+ TR+V I+ PAR   Q G     KWK+ F + ++WENPLMGW+ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWSSTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWDYVVKKRHTPLLKVKAYADNFKW 147
           P +N+    L+F ++E A AFAEK+GW + V++R  P  K K+Y  NF W
Sbjct: 120 PLSNL---VLTFSTKEDAVAFAEKNGWSFDVEERKVPKPKSKSYGANFSW 166

BLAST of Moc01g26280 vs. ExPASy Swiss-Prot
Match: Q0MQH0 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla gorilla gorilla OX=9595 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 9.8e-24
Identity = 51/110 (46.36%), Postives = 73/110 (66.36%), Query Frame = 0

Query: 38  EIGMVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGD 97
           +I  ++G+PEEH+ TR+V I+ PAR   Q G     KWK+ F + ++WENPLMGW ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWDYVVKKRHTPLLKVKAYADNFKW 147
           P +N+    L+F ++E A +FAEK+GW Y +++R  P  K K+Y  NF W
Sbjct: 120 PLSNM---VLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANFSW 166

BLAST of Moc01g26280 vs. ExPASy TrEMBL
Match: A0A6J1BSY3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111005430 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.2e-86
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA
Sbjct: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 155

BLAST of Moc01g26280 vs. ExPASy TrEMBL
Match: A0A6J1HE01 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111463327 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.2e-80
Identity = 146/155 (94.19%), Postives = 149/155 (96.13%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWN  C LRGT+APLCRSFSADSLV+VKPGEIGMVSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGW+YVVKKRHTPLLK KAYADNFKWKG PE KEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHPEPKEA 155

BLAST of Moc01g26280 vs. ExPASy TrEMBL
Match: A0A6J1KR46 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111496890 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.2e-80
Identity = 146/155 (94.19%), Postives = 149/155 (96.13%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWN  C LRGT+APLCRSFSADSLV+VKPGEIGMVSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGW+YVVKKRHTPLLK KAYADNFKWKG PE KEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHPEPKEA 155

BLAST of Moc01g26280 vs. ExPASy TrEMBL
Match: A0A6J1IIF4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111477781 PE=3 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.2e-78
Identity = 142/155 (91.61%), Postives = 150/155 (96.77%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW++   LRGT+APLCRSFS+DSLVQ+KPGEIGMVSGIP+EHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSNSRALRGTVAPLCRSFSSDSLVQLKPGEIGMVSGIPDEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAK FA+K
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKEFADK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGW+YVVKKRHTPLLKVKAYADNFKWKG PEAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGHPEAKEA 155

BLAST of Moc01g26280 vs. ExPASy TrEMBL
Match: A0A6J1GDQ9 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111453236 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 5.5e-78
Identity = 141/155 (90.97%), Postives = 150/155 (96.77%), Query Frame = 0

Query: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW++   +RGT+APLCRSFS+DSLVQ+KPGEIGMVSGIP+EHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSNSRAIRGTVAPLCRSFSSDSLVQLKPGEIGMVSGIPDEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAK FA+K
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKEFADK 120

Query: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEAKEA 156
           HGW+YVVKKRHTPLLKVKAYADNFKWKG PEAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKVKAYADNFKWKGHPEAKEA 155

BLAST of Moc01g26280 vs. TAIR 10
Match: AT5G67590.1 (NADH-ubiquinone oxidoreductase-related )

HSP 1 Score: 222.6 bits (566), Expect = 2.2e-58
Identity = 103/139 (74.10%), Postives = 122/139 (87.77%), Query Frame = 0

Query: 15  LRGTLAPLCRSFSADSLVQ--VKPGEIGMVSGIPEEHLTRRVVIYSPARTASQQGSGKVG 74
           +  TL  + R F+ D++V+   K GEIG VSGIPEEHL+R+V+IYSPARTA+Q GSGK+G
Sbjct: 13  IAATLRRVARPFATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLG 72

Query: 75  KWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWDYVVKKRHT 134
           KWKINF+ST KWENPLMGWTSTGDPYANVGDSAL+FDSEEAAK+FAE+HGWDY VKK +T
Sbjct: 73  KWKINFVSTLKWENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNT 132

Query: 135 PLLKVKAYADNFKWKGPPE 152
           PLLKVK+Y+DNFKWKG P+
Sbjct: 133 PLLKVKSYSDNFKWKGNPQ 151

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132610.16.7e-86100.00NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Momordica ... [more]
XP_038881462.15.8e-8294.84NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa ... [more]
XP_022962977.12.4e-8094.19NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucur... [more]
XP_022977437.16.7e-7891.61NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucur... [more]
XP_022950018.11.1e-7790.97NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucur... [more]
Match NameE-valueIdentityDescription
Q9FJW43.0e-5774.10NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidop... [more]
Q5XIF32.0e-2448.18NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus n... [more]
Q023755.8e-2447.27NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taur... [more]
Q66XS77.5e-2447.27NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gekko ja... [more]
Q0MQH09.8e-2446.36NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla ... [more]
Match NameE-valueIdentityDescription
A0A6J1BSY33.2e-86100.00NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Momordic... [more]
A0A6J1HE011.2e-8094.19NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit... [more]
A0A6J1KR461.2e-8094.19NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit... [more]
A0A6J1IIF43.2e-7891.61NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit... [more]
A0A6J1GDQ95.5e-7890.97NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
AT5G67590.12.2e-5874.10NADH-ubiquinone oxidoreductase-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006885NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrialPFAMPF04800ETC_C1_NDUFA4coord: 53..147
e-value: 4.4E-33
score: 113.4
IPR006885NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrialPANTHERPTHR12219NADH-UBIQUINONE OXIDOREDUCTASEcoord: 23..154
IPR038532NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamilyGENE3D3.30.160.190atu1810 like domaincoord: 52..127
e-value: 1.3E-34
score: 119.9
NoneNo IPR availablePANTHERPTHR12219:SF23NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL-LIKEcoord: 23..154

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g26280.1Moc01g26280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0070469 respirasome
molecular_function GO:0016651 oxidoreductase activity, acting on NAD(P)H