Moc01g25400 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g25400
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Lipase_3 domain-containing protein
Location	chr1: 18100943 .. 18103576 (-)
RNA-Seq Expression	Moc01g25400
Synteny	Moc01g25400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCTGCTCCTGCTTGTAACTGTAACAAGGGCTTCTCCACCAGCTATATGCTTCTGAAGCCGGAAGAAGTGAAGTTCTTTGATCTGGGGCGCCTCCTGTTTTCTAGTAACCTCAAGAAGAGAAAATTCGTGGACTCCAGCCACGCAAGAGAATACAATTTCTGGCGAAGATTCTTCATCTTCCTCTCCATCATCATCCTCAAGCTTCTGCGCCTCGTCGCCACTCCGCTCGCTTACTTAGGCTTCTGCCTCGAGTTCTGCCTCAACTTTTTGTCTGCCAACCAAGGCTTTTCCGGCATCCTCCTCAATATCTTACGCTGTATGTCCCCGGCCATCCTTTCCATCATTTTCTTCTGATCTCTCTTCCCATTCCCACGTTTTTTAATTCATTTTTTCAAATTTCAACTCCACAAACCATTTACTCGAAACTATATTTATATCCTTTCAGAATATTTTTAAACTATTTACAACTTTACTTCCTCAGCTTTCACCTTCTTTTCTACCCATTTTGTTTTTTTCTTTGAGCAAAGTATGTGACCCACATATATTTCTTATTTCTATAAGATGGAAATAATTGTTTGTGTTTGTTTTATATATCATATCTTCCGCGTCTGATCCGGGCAGACTCTGTCGTGTCTACAAATATTATATATATATATATATATATATATATAATACACGCGATGTATTGATCTCTATCTTCTATCATGTATGTGACGATATTTTAATATTTTCGATTATTTTAAAAAAGCCCAATATTTTAAATTAGGATTAAATACTATTTTCAATTTTTTTAATTATATATTTTACTTCAATTAACTTTCAACAGATTTTAAAAATAATATCCACTGTCAATTTAGAGTTAACATATATGGAAAAGAAAAAAGTTGCAAAAGTATTTTTCTTAAAAGGAAAATACTTAGCTTTTTAACTGTCACGATCAAATTAACAACTTGTTATTTATATTTTTTAAAGTTTATGTTTTTGTGTATCTTACTCTAAATATTTAATACTGGTGATTTGTATCTTCCAATTTGAAAAATATATTCACATGAAATGGGAAATGATTAAAGTAATTACAAAAACAATTTTCAAAATTCATTAGAACTTTAAATTTGAAGATCCAAGAATACACAGATTAAAATAGAATAGGTGGTAGATGGAAATTGAAGGTTTGAACTAACAAGTAAAGAAGATTTATGATGCAGTGAAATTGATAATACCGGAGAGTTCATCGGCGGAGTACTTGTCGTTGATTGGTCATTTGGACAGCAGAGTGACATTGGACAAAAGCATAAAGCGAGGCGATGTCAACTACTTCGGAGCTCTCTGCATGATGGCTTCCAAAGTGGCGTACGAGAATCAAGCTCACGTTATTCAAACTGTCAACCATGTCTGGAAGGTTCATATCTTTAATTTTCATCCAATCCAATCCAATGACCAATCATTTCTATAATCATTAACTAATTTTTAATTTAACTTGTTGGTTTCATCTTTTTTCCTGCAGATGGAGTTCTTGGGGTTTTTCAACTTTTGGAATGGTAAAAATTAAACCTAATTAAAGAAAATTCTTTTTCCACCTTAGAATTGATTTTTTATTTAATTTAATTGAATGTTTGAGAATTGTATTTATTTAATTACTAGAATATCAAGAAAAATGCTCGACCCAAGCGTTCATGATGCGCGACAGAACAGTGGATCACGACACCATCATCATATCCTTCAGAGGCACGGAGCCGTTCAACGCCGACGACTGGAGTTCCGACTTCGACATCTCTTGGTACGAGCTCCAAGGCATCGGAAAAATCCACGGCGGATTCATGAAAGCCCTGGGCCTGCAGAAGAGCCTTGGCTGGCCCAAACAACTCCCGGCCCGCCACGGCCCAGCACGCCCTTTGGCCTACTACACCCTCCGAGAGAGGCTCACGGAGCTTCTCCAACAAAGTGAGCGGAGCAGATTCGTGGTGACTGGCCACAGCCTGGGCGGCGCCCTGGCCATCCTATTTCCTGCGATTCTGGCCTTCCACGGAGACAAACTGCTGCTCCAGAGATTAGAGGGAGTGTACACTTTTGGACAGCCTCGCGTGGGGGACGCCAAATTCGGAGAGTTCATGGTCAGATCACTGTGGCAGTACAAGATTCGTTACTACAGATTCGTTTATGGGTTCGACATGGTCCCTCGATTACCGCTGGACGACAAGGCTTTGATGTTCAAGCACTTCGGCCCATGTATCTATTTCGACTGGAACTATGTTGCGCAGGTAATTAATTACATTATTATTATAATAATGAAATTACGTAGCCTTTTAATTAATTTAATTTTGAGGGCGTCAGGTTCTGGAAGAGGAGCCATTCAAGAACTATTTCTCGGTGGTGGGAGCGATACTGATGAGAATACAAGCATGCTTGGAGATAGGGAGAAGCTTCACAATTGCGTGGAGAAGGGGGAAGGAGTACGAGGAAAGAGTGTTTTTGAGAATGGTGAGATTATTTGGGGTGTTATTGCCTGGAATTCCTGCTCATTGTCCCCAAGATTATGTTAATTCCACTCGATTGGGGTCAACCGAAATCTTCGCTTCTCCTCCTCTCACAACTCGTCTCCACAACGAGTACGATGTCAAAATTCAGTAG

mRNA sequence

ATGGCTGCTGCTCCTGCTTGTAACTGTAACAAGGGCTTCTCCACCAGCTATATGCTTCTGAAGCCGGAAGAAGTGAAGTTCTTTGATCTGGGGCGCCTCCTGTTTTCTAGTAACCTCAAGAAGAGAAAATTCGTGGACTCCAGCCACGCAAGAGAATACAATTTCTGGCGAAGATTCTTCATCTTCCTCTCCATCATCATCCTCAAGCTTCTGCGCCTCGTCGCCACTCCGCTCGCTTACTTAGGCTTCTGCCTCGAGTTCTGCCTCAACTTTTTGTCTGCCAACCAAGGCTTTTCCGGCATCCTCCTCAATATCTTACGCTTGAAATTGATAATACCGGAGAGTTCATCGGCGGAGTACTTGTCGTTGATTGGTCATTTGGACAGCAGAGTGACATTGGACAAAAGCATAAAGCGAGGCGATGTCAACTACTTCGGAGCTCTCTGCATGATGGCTTCCAAAGTGGCGTACGAGAATCAAGCTCACGTTATTCAAACTGTCAACCATGTCTGGAAGATGGAGTTCTTGGGGTTTTTCAACTTTTGGAATGAATATCAAGAAAAATGCTCGACCCAAGCGTTCATGATGCGCGACAGAACAGTGGATCACGACACCATCATCATATCCTTCAGAGGCACGGAGCCGTTCAACGCCGACGACTGGAGTTCCGACTTCGACATCTCTTGGTACGAGCTCCAAGGCATCGGAAAAATCCACGGCGGATTCATGAAAGCCCTGGGCCTGCAGAAGAGCCTTGGCTGGCCCAAACAACTCCCGGCCCGCCACGGCCCAGCACGCCCTTTGGCCTACTACACCCTCCGAGAGAGGCTCACGGAGCTTCTCCAACAAAGTGAGCGGAGCAGATTCGTGGTGACTGGCCACAGCCTGGGCGGCGCCCTGGCCATCCTATTTCCTGCGATTCTGGCCTTCCACGGAGACAAACTGCTGCTCCAGAGATTAGAGGGAGTGTACACTTTTGGACAGCCTCGCGTGGGGGACGCCAAATTCGGAGAGTTCATGGTCAGATCACTGTGGCAGTACAAGATTCGTTACTACAGATTCGTTTATGGGTTCGACATGGTCCCTCGATTACCGCTGGACGACAAGGCTTTGATGTTCAAGCACTTCGGCCCATGTATCTATTTCGACTGGAACTATGTTGCGCAGGTTCTGGAAGAGGAGCCATTCAAGAACTATTTCTCGGTGGTGGGAGCGATACTGATGAGAATACAAGCATGCTTGGAGATAGGGAGAAGCTTCACAATTGCGTGGAGAAGGGGGAAGGAGTACGAGGAAAGAGTGTTTTTGAGAATGGTGAGATTATTTGGGGTGTTATTGCCTGGAATTCCTGCTCATTGTCCCCAAGATTATGTTAATTCCACTCGATTGGGGTCAACCGAAATCTTCGCTTCTCCTCCTCTCACAACTCGTCTCCACAACGAGTACGATGTCAAAATTCAGTAG

Coding sequence (CDS)

ATGGCTGCTGCTCCTGCTTGTAACTGTAACAAGGGCTTCTCCACCAGCTATATGCTTCTGAAGCCGGAAGAAGTGAAGTTCTTTGATCTGGGGCGCCTCCTGTTTTCTAGTAACCTCAAGAAGAGAAAATTCGTGGACTCCAGCCACGCAAGAGAATACAATTTCTGGCGAAGATTCTTCATCTTCCTCTCCATCATCATCCTCAAGCTTCTGCGCCTCGTCGCCACTCCGCTCGCTTACTTAGGCTTCTGCCTCGAGTTCTGCCTCAACTTTTTGTCTGCCAACCAAGGCTTTTCCGGCATCCTCCTCAATATCTTACGCTTGAAATTGATAATACCGGAGAGTTCATCGGCGGAGTACTTGTCGTTGATTGGTCATTTGGACAGCAGAGTGACATTGGACAAAAGCATAAAGCGAGGCGATGTCAACTACTTCGGAGCTCTCTGCATGATGGCTTCCAAAGTGGCGTACGAGAATCAAGCTCACGTTATTCAAACTGTCAACCATGTCTGGAAGATGGAGTTCTTGGGGTTTTTCAACTTTTGGAATGAATATCAAGAAAAATGCTCGACCCAAGCGTTCATGATGCGCGACAGAACAGTGGATCACGACACCATCATCATATCCTTCAGAGGCACGGAGCCGTTCAACGCCGACGACTGGAGTTCCGACTTCGACATCTCTTGGTACGAGCTCCAAGGCATCGGAAAAATCCACGGCGGATTCATGAAAGCCCTGGGCCTGCAGAAGAGCCTTGGCTGGCCCAAACAACTCCCGGCCCGCCACGGCCCAGCACGCCCTTTGGCCTACTACACCCTCCGAGAGAGGCTCACGGAGCTTCTCCAACAAAGTGAGCGGAGCAGATTCGTGGTGACTGGCCACAGCCTGGGCGGCGCCCTGGCCATCCTATTTCCTGCGATTCTGGCCTTCCACGGAGACAAACTGCTGCTCCAGAGATTAGAGGGAGTGTACACTTTTGGACAGCCTCGCGTGGGGGACGCCAAATTCGGAGAGTTCATGGTCAGATCACTGTGGCAGTACAAGATTCGTTACTACAGATTCGTTTATGGGTTCGACATGGTCCCTCGATTACCGCTGGACGACAAGGCTTTGATGTTCAAGCACTTCGGCCCATGTATCTATTTCGACTGGAACTATGTTGCGCAGGTTCTGGAAGAGGAGCCATTCAAGAACTATTTCTCGGTGGTGGGAGCGATACTGATGAGAATACAAGCATGCTTGGAGATAGGGAGAAGCTTCACAATTGCGTGGAGAAGGGGGAAGGAGTACGAGGAAAGAGTGTTTTTGAGAATGGTGAGATTATTTGGGGTGTTATTGCCTGGAATTCCTGCTCATTGTCCCCAAGATTATGTTAATTCCACTCGATTGGGGTCAACCGAAATCTTCGCTTCTCCTCCTCTCACAACTCGTCTCCACAACGAGTACGATGTCAAAATTCAGTAG

Protein sequence

MAAAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFFIFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHNEYDVKIQ

Homology

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BLAST of Moc01g25400 vs. NCBI nr
Match: XP_022132553.1 (uncharacterized protein LOC111005383 [Momordica charantia])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.3e-288
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MAAAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFF 60
           MAAAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFF
Sbjct: 1   MAAAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFF 60

Query: 61  IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY 120
           IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY
Sbjct: 61  IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY 120

Query: 121 LSLIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFN 180
           LSLIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFN 180

Query: 181 FWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHG 240
           FWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHG
Sbjct: 181 FWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHG 240

Query: 241 GFMKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGAL 300
           GFMKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGAL
Sbjct: 241 GFMKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGAL 300

Query: 301 AILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDM 360
           AILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDM
Sbjct: 301 AILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDM 360

Query: 361 VPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSF 420
           VPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSF
Sbjct: 361 VPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSF 420

Query: 421 TIAWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHN 480
           TIAWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHN
Sbjct: 421 TIAWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHN 480

Query: 481 EYDVKIQ 488
           EYDVKIQ
Sbjct: 481 EYDVKIQ 487

[Close]

BLAST of Moc01g25400 vs. NCBI nr
Match: XP_038883943.1 (triacylglycerol lipase OBL1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 815.5 bits (2105), Expect = 2.5e-232
Identity = 391/482 (81.12%), Postives = 431/482 (89.42%), Query Frame = 0


Query: 6   ACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFFIFLSI 65
           A NCN GFS SYMLL  EEVKF DL RLLFSSNLKKR+FVDSS ARE+NFW RFFIFLSI
Sbjct: 18  AYNCNSGFSNSYMLLNTEEVKFLDLFRLLFSSNLKKRRFVDSSRAREHNFWHRFFIFLSI 77

Query: 66  IILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIG 125
           I+LKLLR    PLA LGF LE  LNFLSAN GFSG+L N LR KL IP+SSSAEYLS+IG
Sbjct: 78  IVLKLLRFFDKPLALLGFLLESSLNFLSANGGFSGVLFNFLRFKLKIPDSSSAEYLSMIG 137

Query: 126 HLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWNEY 185
           HLDSRVTLDKSIK GDVNYFGALCMMASK+AYEN+AHV QTVN++WKMEFLGFFNFWN+Y
Sbjct: 138 HLDSRVTLDKSIKPGDVNYFGALCMMASKLAYENEAHVTQTVNNIWKMEFLGFFNFWNDY 197

Query: 186 QEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKA 245
           QEKCSTQAFMMRD+ VDHDTII+SFRGTEPFNADDWSSDFDISWYE++G+GKIHGGFMKA
Sbjct: 198 QEKCSTQAFMMRDKKVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIKGMGKIHGGFMKA 257

Query: 246 LGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFP 305
           LGLQK +GWPK++  +    R LAYYTLRE+L EL++++ER+RFVVTGHSLGGALAILFP
Sbjct: 258 LGLQKCIGWPKEIKRQDHQRRSLAYYTLREKLKELMKENERTRFVVTGHSLGGALAILFP 317

Query: 306 AILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLP 365
           +IL FH +KLLL+RLEGVYTFGQPRVGD +FGEFMV+SL QY IRYYRFVYGFDMVPRLP
Sbjct: 318 SILVFHEEKLLLERLEGVYTFGQPRVGDREFGEFMVKSLSQYNIRYYRFVYGFDMVPRLP 377

Query: 366 LDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWR 425
           LDDKALMFKHFGPCIYFDWNY AQ+LEEEPFKNYFS+VGA+LMRI ACLEIGRSFTIAWR
Sbjct: 378 LDDKALMFKHFGPCIYFDWNYDAQILEEEPFKNYFSIVGAVLMRIHACLEIGRSFTIAWR 437

Query: 426 RGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHNEYDVK 485
           RGK+YEE+VF R+VRLFG+LLPGIPAHCPQDYVNSTRLGST+         RLH+EYDVK
Sbjct: 438 RGKDYEEKVFFRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTD---------RLHSEYDVK 490

Query: 486 IQ 488
           IQ
Sbjct: 498 IQ 490

[Close]

BLAST of Moc01g25400 vs. NCBI nr
Match: XP_008440283.1 (PREDICTED: uncharacterized protein LOC107990286 [Cucumis melo])

HSP 1 Score: 813.5 bits (2100), Expect = 9.6e-232
Identity = 392/481 (81.50%), Postives = 430/481 (89.40%), Query Frame = 0


Query: 8   NCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFFIFLSIII 67
           NCN GFS+SYMLL PEEVKF DL RLLFSSNLKKR+FVDSSHARE+NFW RFFIFLSI++
Sbjct: 18  NCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIVV 77

Query: 68  LKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIGHL 127
           LKLLR    PLA LGF LE  LNFLS N GFSGILLN  RL+L IP+SSSAEYLS+IGHL
Sbjct: 78  LKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGHL 137

Query: 128 DSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWNEYQE 187
           DSRVTLD SIK GDVNYFGALCMMASK++YEN+AHV Q V  VWKMEFLGFFNFWN+YQE
Sbjct: 138 DSRVTLDSSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQE 197

Query: 188 KCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKALG 247
           KCSTQAFMMRD+ VDHDTI++SFRGTEPFNADDWSSDFDISWYE++GIGKIHGGFMKALG
Sbjct: 198 KCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALG 257

Query: 248 LQKSLGWPKQLPAR-HGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPA 307
           LQK +GWPK++  + H   RPLAYYTLRE+L EL++++ER+RFVVTGHSLGGALAILFP+
Sbjct: 258 LQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFPS 317

Query: 308 ILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPL 367
           IL FH +KLLL+RLEGVYTFGQPRVGD  FGEFMV+SL QYKIRYYRFVYGFDMVPRLPL
Sbjct: 318 ILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLPL 377

Query: 368 DDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRR 427
           DDKALMFKHFGPCIYFDWNYVAQ+LEEEPFKNYFS+VGAILMRI ACLEIGRSFTI+WRR
Sbjct: 378 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWRR 437

Query: 428 GKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHNEYDVKI 487
           GKEYEE+VFLR+VRLFG+LLPGIPAHCPQDYVNSTRLGST  F S         EYDVK+
Sbjct: 438 GKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTSHFLS---------EYDVKV 489

[Close]

BLAST of Moc01g25400 vs. NCBI nr
Match: KAG6595033.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027056.1 Lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 813.1 bits (2099), Expect = 1.3e-231
Identity = 396/487 (81.31%), Postives = 432/487 (88.71%), Query Frame = 0


Query: 1   MAAAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFF 60
           MAA  A NCN GFS SYMLLKPEEVKF DL RLLFSSNLKKR+F+DSSHAREYNFW RFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY 120
           IFLSI++LKLL  +A PLA  GF LE  LN LSAN GF GILLNILRLKL IP+SSSAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFN 180
           LSLIGHLDSRVTLD+SIK GDVNYFGALCMMASKV YEN+AHV+QTVN VWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHG 240
           FWN YQEKCSTQAFMMRD+ VDHDTII+SFRGTEPFNADDWSSDFDISWYE++GIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGAL 300
           GFMKALGLQK+ GWPK++  R    RPLAYYTLRERL ELL++ ER+RFVVTGHSLGGAL
Sbjct: 241 GFMKALGLQKTHGWPKEID-REDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGAL 300

Query: 301 AILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDM 360
           AILFP+ILAFH +KLLL+RLEGVYTFGQPRVGD+KFGEFMV+SL QYKIRYYRFVYGFDM
Sbjct: 301 AILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDM 360

Query: 361 VPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSF 420
           VPRLPLD+  LM+KHFGPCIYF+WNYVAQ+LEEEPFKNYFS+VGA+LMRI AC EIGRSF
Sbjct: 361 VPRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSF 420

Query: 421 TIAWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHN 480
           TI WRRGKEYEER+  R+VRL G+LLPGIPAHCPQDYVNSTRLGS         T  L++
Sbjct: 421 TIGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGS---------TGSLNS 477

Query: 481 EYDVKIQ 488
           EYDVKI+
Sbjct: 481 EYDVKIE 477

[Close]

BLAST of Moc01g25400 vs. NCBI nr
Match: XP_023543970.1 (uncharacterized protein LOC111803685 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 811.6 bits (2095), Expect = 3.7e-231
Identity = 392/484 (80.99%), Postives = 430/484 (88.84%), Query Frame = 0


Query: 4   APACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFFIFL 63
           A AC+CN GFS SYMLLKPEEVKFFDL RLLFSSNLKKRKFVDS+ ARE+NFW RFFIFL
Sbjct: 3   ASACSCNSGFSNSYMLLKPEEVKFFDLLRLLFSSNLKKRKFVDSTSAREHNFWHRFFIFL 62

Query: 64  SIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSL 123
           SII+LKLLR  A PLA LGF LE  LNF+SAN GFSGILLNILR KLIIP+SSSAEYLS+
Sbjct: 63  SIIVLKLLRFFAKPLALLGFFLESWLNFISANGGFSGILLNILRFKLIIPDSSSAEYLSI 122

Query: 124 IGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWN 183
           IGHLDSRV LD+ IK GDVNYFGALCMMASK+ YEN+A+V QTVNHVWKME LGFFNFWN
Sbjct: 123 IGHLDSRVRLDEKIKAGDVNYFGALCMMASKLVYENEAYVTQTVNHVWKMELLGFFNFWN 182

Query: 184 EYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFM 243
           +YQEK STQAFMMRD+  DHDTII+SFRGTEPFNADDWSSDFDISWYE+QGIGKIHGGFM
Sbjct: 183 DYQEKFSTQAFMMRDKNADHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFM 242

Query: 244 KALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAIL 303
           KALGL KS+GWPK++  +    RPLAYYT+RE+L E+L+++ER+RFVVTGHSLGGALAIL
Sbjct: 243 KALGLHKSIGWPKEIDPQQ-QRRPLAYYTIREKLREVLKENERTRFVVTGHSLGGALAIL 302

Query: 304 FPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPR 363
           FP IL FH +KLLL+RLEGVYTFGQPRVGD +FGEFM++SL QYKIRYYRFVYGFDMVPR
Sbjct: 303 FPFILVFHEEKLLLERLEGVYTFGQPRVGDGEFGEFMMKSLSQYKIRYYRFVYGFDMVPR 362

Query: 364 LPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIA 423
           LPLDDKALMFKHFG CIYFDWNYVAQ+LEEEPFKNYFS+VGA+LMRI AC EIGRSFTI 
Sbjct: 363 LPLDDKALMFKHFGRCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIG 422

Query: 424 WRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHNEYD 483
           WRRGKEYEERV LR+VRLFG+LLPG+PAHCPQDYVNSTRLGS +         R  NEYD
Sbjct: 423 WRRGKEYEERVLLRIVRLFGLLLPGVPAHCPQDYVNSTRLGSAD---------RFLNEYD 476

Query: 484 VKIQ 488
           VKIQ
Sbjct: 483 VKIQ 476

[Close]

BLAST of Moc01g25400 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.0e-99
Identity = 202/497 (40.64%), Postives = 290/497 (58.35%), Query Frame = 0


Query: 10  NKGFSTSYMLLKPEEVKFFDLGRL-LFSSNLKKRKFVDSSHAREYNFW--------RRFF 69
           N G  ++Y++++P    + DL R  +      K KF++    RE++           R+ 
Sbjct: 12  NPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWV 71

Query: 70  IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY 129
           I +SI++ K++RL+ TP+ + GF ++F LN  SAN GF G+LL +++ K++IPE  S  +
Sbjct: 72  IVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTF 131

Query: 130 LSLIGHLDSRVTLDK------------SIKRGDVNY-FGA-----LCMMASKVAYENQAH 189
           +S IG LD R++L K            S+  G V    G+     LC+MASK+AYEN   
Sbjct: 132 VSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKV 191

Query: 190 VIQTVNHVWKMEFLGFFNFWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWS 249
           V   V+  WKM  + F + WN+YQ++ STQ F+  D+  D + I+ISFRGTEPF+ADDW 
Sbjct: 192 VENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWG 251

Query: 250 SDFDISWYELQGIGKIHGGFMKALGL---------------QKSLGWPKQLPARHGPARP 309
           +DFD SWYE+  +GK+H GF++A+GL               Q S                
Sbjct: 252 TDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVER 311

Query: 310 LAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAILAFHGDKLLLQRLEGVYTFG 369
            AYY +R  L  LL + E +RFVVTGHSLGGALAILFP +L  + +  +++RL GVYTFG
Sbjct: 312 SAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFG 371

Query: 370 QPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFGPCIYFDWNYV 429
           QPR+G+ + G FM   L Q   RY+R VY  D+VPRLP DDK  ++KHFG C+++D  Y 
Sbjct: 372 QPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYN 431

Query: 430 AQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRRGKEYEERVFLRMVRLFGVLLP 465
               E+EP  N + +   IL  + A  E+ R  T+ +  G +Y+E  F  + RL G+++P
Sbjct: 432 ETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIP 491

[Close]

BLAST of Moc01g25400 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.6e-96
Identity = 197/477 (41.30%), Postives = 274/477 (57.44%), Query Frame = 0


Query: 58  RFFIFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSS 117
           R+ IF+SII+ KL+ +   P+ + G+ +EF LN  S N  F G+L NIL  K+++P   S
Sbjct: 86  RWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGKVVMPHRGS 145

Query: 118 AEYLSLIGHLDSRVTLDKS----IKRGDVNYFGA----------LCMMASKVAYENQAHV 177
             ++S IGHLD R+ L KS     + G+ +++            LCMMASK+AYEN+  V
Sbjct: 146 ETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEKVV 205

Query: 178 IQTVNHVWKMEFLGFFNFWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSS 237
              VN  WKM F+ F+N WN+++++ STQ F++ D+  D + I++SFRGTEPF+ADDW +
Sbjct: 206 RNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDWIT 265

Query: 238 DFDISWYELQGIGKIHGGFMKALGL----------------------------------- 297
           DFD SWYE+  +GK+H GF++ALGL                                   
Sbjct: 266 DFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPSES 325

Query: 298 QKSLGWPKQLPARHG------PARP---------------LAYYTLRERLTELLQQSERS 357
            KS        A  G        RP                AYY +R +L  LL++ + +
Sbjct: 326 SKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHKNA 385

Query: 358 RFVVTGHSLGGALAILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQY 417
           +FVVTGHSLGGALAILFPA+L  H +  +++RL G+YT+GQPRVG+ + G FM   L   
Sbjct: 386 KFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLEHP 445

Query: 418 KIRYYRFVYGFDMVPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAIL 465
             +Y+R VY  D+VPRLP D+K  +FKHFG C Y++  Y+ Q + EEP  NYF +   + 
Sbjct: 446 VPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFGMRFLVP 505

[Close]

BLAST of Moc01g25400 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 5.9e-83
Identity = 198/506 (39.13%), Postives = 269/506 (53.16%), Query Frame = 0


Query: 14  STSYMLLKPEEVKFFDLGRLLFSSNL-KKRKFVDSSHAR------EYNFWRRFFIFLSII 73
           STS++++ P+E  F DL + +  S+L    KF  +S  R       Y+ W      +S+ 
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWP-----VSVF 68

Query: 74  ILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIGH 133
           I K+L+L   P A LG   +F LNF   N GF GIL NI  ++L IP+   A+++S IG+
Sbjct: 69  ICKILQLFQMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLIRLKIPKRGEADFISTIGY 128

Query: 134 LDSRVTL------------------DKSIKRG-DVNYFGA--------LCMMASKVAYEN 193
           LDSR+ L                  + S+K G +    GA        LC+MASK+AYEN
Sbjct: 129 LDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYEN 188

Query: 194 QAHVIQTVNHVWKMEFLGFFNFWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNAD 253
              V + V   WKM F+  +   N +Q+  +T AF+  D+  D + I+ISFRGT PF+  
Sbjct: 189 TKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIP 248

Query: 254 DWSSDFDISWYELQGIGKIHGGFMKALGL--------------QKSLGWPKQL------- 313
           +W +DFD S   L   G +H GF++A+GL               KS G   +L       
Sbjct: 249 NWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMA 308

Query: 314 PARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAILAFHGDKLLLQ 373
           P     A    Y+     L  LL+  + ++FVVTGHSLGGALAILF  IL    +  +L 
Sbjct: 309 PDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLD 368

Query: 374 RLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFGP 433
           RL  VYTFGQPR+G+   G FM   L   + RY+R VY  DMVPR+P DD    F+HFG 
Sbjct: 369 RLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGT 428

Query: 434 CIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRRGKEYEERVFLRM 465
           CIY+D  +     +EEP +N F +  AI   I A  E+ RSF +    G EY+E    RM
Sbjct: 429 CIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRM 488

[Close]

BLAST of Moc01g25400 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 8.2e-08
Identity = 54/172 (31.40%), Postives = 85/172 (49.42%), Query Frame = 0


Query: 194 FMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKALGLQKSLG 253
           F+  D T  +  I++SFRG+   + ++W  + +   ++L+ I  I  G     G   S  
Sbjct: 88  FLALDNT--NKLIVLSFRGSR--SIENWIGNLN---FDLKEINDICSGCRGHDGFTSS-- 147

Query: 254 WPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAILAFHGD 313
           W           R +A  TLR+++ + +++    R V TGHSLGGALA +  A L  +G 
Sbjct: 148 W-----------RSVA-DTLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGY 207

Query: 314 KLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLP 366
            +       V+++G PRVG+  F EF+     Q     YR  +  D+VPRLP
Sbjct: 208 DI------DVFSYGAPRVGNRAFAEFLT---VQTGGTLYRITHTNDIVPRLP 229

[Close]

BLAST of Moc01g25400 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 4.1e-07
Identity = 54/176 (30.68%), Postives = 79/176 (44.89%), Query Frame = 0


Query: 205 TIIISFRGTEPFNADDWSSDF---DISWYELQGIGKIHGGFMKALGLQKSLGWPKQLPAR 264
           TI I FRG+      +W +D     +S+  + G  K+H GF+ + G  ++          
Sbjct: 168 TIYIVFRGSSSIR--NWIADLTFVPVSYPPVSGT-KVHKGFLDSYGEVQN---------- 227

Query: 265 HGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAILAFHGDKLLLQRLE 324
                      L   + +  +Q    +  VTGHSLGGA A+L  A+  +  ++ L     
Sbjct: 228 ----------ELVATVLDQFKQYPSYKVAVTGHSLGGATALLC-ALDLYQREEGLSSSNL 287

Query: 325 GVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFG 378
            +YT GQPRVGD  F  ++V +     I Y R V   D+VP LP    A  F H G
Sbjct: 288 FLYTQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLP--PAAFGFLHAG 313

[Close]

BLAST of Moc01g25400 vs. ExPASy TrEMBL
Match: A0A6J1BWK4 (uncharacterized protein LOC111005383 OS=Momordica charantia OX=3673 GN=LOC111005383 PE=4 SV=1)

HSP 1 Score: 1001.1 bits (2587), Expect = 1.6e-288
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MAAAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFF 60
           MAAAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFF
Sbjct: 1   MAAAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFF 60

Query: 61  IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY 120
           IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY
Sbjct: 61  IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY 120

Query: 121 LSLIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFN 180
           LSLIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFN 180

Query: 181 FWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHG 240
           FWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHG
Sbjct: 181 FWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHG 240

Query: 241 GFMKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGAL 300
           GFMKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGAL
Sbjct: 241 GFMKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGAL 300

Query: 301 AILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDM 360
           AILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDM
Sbjct: 301 AILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDM 360

Query: 361 VPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSF 420
           VPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSF
Sbjct: 361 VPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSF 420

Query: 421 TIAWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHN 480
           TIAWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHN
Sbjct: 421 TIAWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHN 480

Query: 481 EYDVKIQ 488
           EYDVKIQ
Sbjct: 481 EYDVKIQ 487

[Close]

BLAST of Moc01g25400 vs. ExPASy TrEMBL
Match: A0A1S3B1H5 (uncharacterized protein LOC107990286 OS=Cucumis melo OX=3656 GN=LOC107990286 PE=4 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 4.7e-232
Identity = 392/481 (81.50%), Postives = 430/481 (89.40%), Query Frame = 0


Query: 8   NCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFFIFLSIII 67
           NCN GFS+SYMLL PEEVKF DL RLLFSSNLKKR+FVDSSHARE+NFW RFFIFLSI++
Sbjct: 18  NCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIVV 77

Query: 68  LKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIGHL 127
           LKLLR    PLA LGF LE  LNFLS N GFSGILLN  RL+L IP+SSSAEYLS+IGHL
Sbjct: 78  LKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGHL 137

Query: 128 DSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWNEYQE 187
           DSRVTLD SIK GDVNYFGALCMMASK++YEN+AHV Q V  VWKMEFLGFFNFWN+YQE
Sbjct: 138 DSRVTLDSSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQE 197

Query: 188 KCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKALG 247
           KCSTQAFMMRD+ VDHDTI++SFRGTEPFNADDWSSDFDISWYE++GIGKIHGGFMKALG
Sbjct: 198 KCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALG 257

Query: 248 LQKSLGWPKQLPAR-HGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPA 307
           LQK +GWPK++  + H   RPLAYYTLRE+L EL++++ER+RFVVTGHSLGGALAILFP+
Sbjct: 258 LQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFPS 317

Query: 308 ILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPL 367
           IL FH +KLLL+RLEGVYTFGQPRVGD  FGEFMV+SL QYKIRYYRFVYGFDMVPRLPL
Sbjct: 318 ILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLPL 377

Query: 368 DDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRR 427
           DDKALMFKHFGPCIYFDWNYVAQ+LEEEPFKNYFS+VGAILMRI ACLEIGRSFTI+WRR
Sbjct: 378 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWRR 437

Query: 428 GKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHNEYDVKI 487
           GKEYEE+VFLR+VRLFG+LLPGIPAHCPQDYVNSTRLGST  F S         EYDVK+
Sbjct: 438 GKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTSHFLS---------EYDVKV 489

[Close]

BLAST of Moc01g25400 vs. ExPASy TrEMBL
Match: A0A6J1GET2 (uncharacterized protein LOC111453276 OS=Cucurbita moschata OX=3662 GN=LOC111453276 PE=4 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.3e-231
Identity = 393/485 (81.03%), Postives = 431/485 (88.87%), Query Frame = 0


Query: 3   AAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFFIF 62
           AA AC+CN GFS SYMLLKPEEVKFFDL RLLFSSNLKKRKFVDS+ ARE+NFW RFFIF
Sbjct: 2   AASACSCNSGFSNSYMLLKPEEVKFFDLLRLLFSSNLKKRKFVDSTSAREHNFWHRFFIF 61

Query: 63  LSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLS 122
           LSI++LKLLR  A PLA LGF LE  LNF+SAN GFSGILLNILR KLIIP+SSSAEYLS
Sbjct: 62  LSIVVLKLLRFFAKPLALLGFFLESWLNFISANGGFSGILLNILRFKLIIPDSSSAEYLS 121

Query: 123 LIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFW 182
           +IGHLDSRV LD+ IK GDVNYFGALCMMASK+AYEN+A V QTVNHVWKME LGFFNFW
Sbjct: 122 IIGHLDSRVRLDEKIKAGDVNYFGALCMMASKLAYENEACVTQTVNHVWKMELLGFFNFW 181

Query: 183 NEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGF 242
           N YQEK STQAFMMRD+  DHDTII+SFRGTEPFNADDWSSDFDISWYE+QGIGKIHGGF
Sbjct: 182 NGYQEKFSTQAFMMRDKNADHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGF 241

Query: 243 MKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAI 302
           MKALGLQKS+GWPK++  +    RPL+YYT+RE+L E+L+++ER+RFVVTGHSLGGALAI
Sbjct: 242 MKALGLQKSIGWPKEIDPQQ-QRRPLSYYTIREKLREVLKENERTRFVVTGHSLGGALAI 301

Query: 303 LFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVP 362
           LFP IL FH +KLLL+RLEGVYTFGQPRVGD +FGEFM++SL QYKIRYYRFVYGFDMVP
Sbjct: 302 LFPFILVFHEEKLLLERLEGVYTFGQPRVGDGEFGEFMMKSLSQYKIRYYRFVYGFDMVP 361

Query: 363 RLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTI 422
           RLPLDDKALMFKHFG CIYFDWNYVAQ+LEEEPFKNYFS+VGA+LMRI AC EIGRSFTI
Sbjct: 362 RLPLDDKALMFKHFGRCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTI 421

Query: 423 AWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHNEY 482
            WRRGKEYEERV LR+VRLFG+LLPG+PAHCPQDYVNSTRLGS +         R  NEY
Sbjct: 422 GWRRGKEYEERVLLRIVRLFGLLLPGVPAHCPQDYVNSTRLGSAD---------RFLNEY 476

Query: 483 DVKIQ 488
           DVKIQ
Sbjct: 482 DVKIQ 476

[Close]

BLAST of Moc01g25400 vs. ExPASy TrEMBL
Match: A0A6J1KN75 (uncharacterized protein LOC111497186 OS=Cucurbita maxima OX=3661 GN=LOC111497186 PE=4 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 6.7e-231
Identity = 394/487 (80.90%), Postives = 431/487 (88.50%), Query Frame = 0


Query: 1   MAAAPACNCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFF 60
           MAA  A NC+ GFS SYMLLKPEEVKF DL RLLFSSNLKKR+F+DSSHAREYNFW RFF
Sbjct: 1   MAARRAYNCSNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY 120
           IFLSI++LKLL  +A PLA  GF LE  LN LSAN GF GILLNILRLKL IP+SSSAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFN 180
           LSLIGHLDSRVTLD+SIK GDVNYFGALCMMASKV YEN+AHV+QTVN VWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHG 240
           FWN YQEKCSTQAFMMRD+ VDHDTII+SFRGTEPFNADDWSSDFDISWYE++GIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGAL 300
           GFMKALGLQK+ GW K++  R    RPLAYYTLRERL ELL++ ER+RFVVTGHSLGGAL
Sbjct: 241 GFMKALGLQKTHGWTKEID-REDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGAL 300

Query: 301 AILFPAILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDM 360
           AILFP ILAFH +KLLL+RLEGVYTFGQPRVGD+KFGEFMV+SL QYKIRYYRFVYGFDM
Sbjct: 301 AILFPTILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDM 360

Query: 361 VPRLPLDDKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSF 420
           VPRLPLD+  LM+KHFGPCIYF+WNYVAQ+LEEEPFKNYFS+VGA+LMRI AC EIGRSF
Sbjct: 361 VPRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSF 420

Query: 421 TIAWRRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHN 480
           TI WRRGKEYEER+  R+VRLFG+LLPGIPAHCPQDYVN+TRLGS         T  L++
Sbjct: 421 TIGWRRGKEYEERMCFRIVRLFGLLLPGIPAHCPQDYVNATRLGS---------TVSLNS 477

Query: 481 EYDVKIQ 488
           EYDVKI+
Sbjct: 481 EYDVKIE 477

[Close]

BLAST of Moc01g25400 vs. ExPASy TrEMBL
Match: A0A0A0KFS7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490940 PE=4 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.3e-230
Identity = 387/480 (80.62%), Postives = 426/480 (88.75%), Query Frame = 0


Query: 8   NCNKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFFIFLSIII 67
           NCN GFS SYMLL PEEVKF DL RLLFSSNLKKR+FVDSSHARE+NFW RFFIFLSI++
Sbjct: 17  NCNGGFSNSYMLLNPEEVKFLDLFRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIVV 76

Query: 68  LKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIGHL 127
           LKLLR    PLA LGF LE  LNFLS N GFSGILLN  RL+L IP +SSAEYLS+IGHL
Sbjct: 77  LKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPNTSSAEYLSMIGHL 136

Query: 128 DSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWNEYQE 187
           DSRVTLD+SIK GDVNYFGALCMMASK++YEN+AHV Q V  VWKMEFLGFFNFWN+YQE
Sbjct: 137 DSRVTLDRSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQE 196

Query: 188 KCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKALG 247
           KCSTQAFMMRDR  +HDTII+SFRGTEPFNADDWSSDFDISWYE++GIGKIHGGFMKALG
Sbjct: 197 KCSTQAFMMRDRKDNHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALG 256

Query: 248 LQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAI 307
           LQK +GWPK++  +     PLAYYTLRE+L EL++++ER+RFVVTGHSLGGALAILFP+I
Sbjct: 257 LQKCVGWPKEMERKGHERHPLAYYTLREKLKELMKENERTRFVVTGHSLGGALAILFPSI 316

Query: 308 LAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPLD 367
           L FH +KLLL+RLEGVYTFGQPRVGD  FGEFMV+SL +YKIRYYRFVYGFDMVPRLPLD
Sbjct: 317 LVFHEEKLLLERLEGVYTFGQPRVGDRTFGEFMVKSLAEYKIRYYRFVYGFDMVPRLPLD 376

Query: 368 DKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRRG 427
           DKALMFKHFGPCIYFDWNYVAQ+LEEEPFKNYFS+VGAILMRI ACLEIGRSFTI+WRRG
Sbjct: 377 DKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWRRG 436

Query: 428 KEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTTRLHNEYDVKIQ 487
           KEYEE+VFLR+VRLFG+LLPGIPAHCPQDYVNSTRLGS         TT  H EYDVK++
Sbjct: 437 KEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGS---------TTPFHTEYDVKVE 487

[Close]

BLAST of Moc01g25400 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 478.8 bits (1231), Expect = 5.2e-135
Identity = 238/454 (52.42%), Postives = 325/454 (71.59%), Query Frame = 0


Query: 17  YMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWRRFFIFLSIIILKLLRLVAT 76
           Y++L+PEE++ ++L RLLFS +++K + VDSS   E++F  R+ IF+S+++LKLLR  + 
Sbjct: 13  YLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEHSFRHRWLIFVSLVLLKLLRFFSK 72

Query: 77  PLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIGHLDSRVTLDKS 136
            LA +G  LEF LNFLS N  FSG+    LR ++++P+ +S  Y S IGHLD+RV+LD +
Sbjct: 73  LLALVGSALEFSLNFLS-NNSFSGL---FLRGEVVMPQRTSENYQSFIGHLDTRVSLDMT 132

Query: 137 IKRGD-VNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWNEYQEKCSTQAFM 196
           + R D   Y+ AL +MASK+AYEN A +   V + W M++LG  ++WNEYQEK +TQAF+
Sbjct: 133 LNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLVDYWNEYQEKETTQAFI 192

Query: 197 M-RDRTV-----DHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKALGLQ 256
           M  D T         T++++FRGTE FN++DW SDFDI+W+EL  IG IHGGFMKALGLQ
Sbjct: 193 MSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPNIGNIHGGFMKALGLQ 252

Query: 257 KSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAILA 316
            +  WPK+  +      PLAYY++R+ L  L+ Q++ ++FV+TGHSLGGALAILF A+L 
Sbjct: 253 NNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGGALAILFTAVLV 312

Query: 317 FHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPLDDK 376
            H +  LL+R++GVYT+GQPRVGD+KFGEFM + L +Y I+YYRFVY  D+VPRLP DDK
Sbjct: 313 IHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNNDIVPRLPYDDK 372

Query: 377 ALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRRGKE 436
            LMFKHFG CIY+D NY A+V+ E+  +N+F + G I M   A LE  RSFTI   +G E
Sbjct: 373 DLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIRSFTIVAEKGSE 432

Query: 437 YEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRL 464
           Y E   L+  R  G+++PG+  H PQDYVN+TRL
Sbjct: 433 YSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRL 462

[Close]

BLAST of Moc01g25400 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 4.0e-127
Identity = 230/455 (50.55%), Postives = 312/455 (68.57%), Query Frame = 0


Query: 16  SYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYN----FWRRFFIFLSIIILKLL 75
           +Y +L P E    DL  LLFSS+L  RKF+ SS  R  +    F RR+ IF+SI+I KL+
Sbjct: 6   NYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQKLI 65

Query: 76  RLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIGHLDSRV 135
            L   PL ++GF L   LN LS+N GF  IL N+ +  +I PE +SA + SL G+LD RV
Sbjct: 66  ILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLNGNLDRRV 125

Query: 136 TLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWNEYQEKCST 195
            L+  ++RG   Y   L +MASK++YEN   V   +++ WKM+ LGF++ WN YQ++ ST
Sbjct: 126 ELNPKVERGSKRYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQKQRST 185

Query: 196 QAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKALGLQKS 255
           +  +++D + D + II+SFRGT+PF+ADDW +D D+SWYE++ +GKIHGGFMKALGLQK 
Sbjct: 186 EVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALGLQKE 245

Query: 256 LGWPKQ--LPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAILA 315
            GWPK+  L          AYYT+R  L E+L Q+  S+F++TGHSLGGALAILF A+L 
Sbjct: 246 -GWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFTAVLV 305

Query: 316 FHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPLDDK 375
            H ++ +L+RLEGVYTFGQPRVGD +FG FM  SL ++ ++Y R+VY  DMVPRLP DDK
Sbjct: 306 MHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLPFDDK 365

Query: 376 ALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRRGKE 435
            LMFKHFG C+Y+D  Y  +V EEEP KNYF++V  +   + A  E+ RSF + + +G E
Sbjct: 366 TLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYWKGGE 425

Query: 436 YEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLG 465
           + E  FLR  R+  +L+PG+PAH P +Y+N T LG
Sbjct: 426 FREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLG 459

[Close]

BLAST of Moc01g25400 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 422.5 bits (1085), Expect = 4.4e-118
Identity = 220/458 (48.03%), Postives = 305/458 (66.59%), Query Frame = 0


Query: 16  SYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSS----HAREYNFWRRFFIFLSIIILKLL 75
           +Y +L P E    DL RLLF S+L+ RKFVD+S      R   F  R+ IF+SI++ KLL
Sbjct: 6   NYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKLL 65

Query: 76  RLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIGHLDSRV 135
            ++  PL++LGF L F LN  S+N GF  I LN+++ + I PE +SA + S+ G+LD +V
Sbjct: 66  IILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTSATFASINGNLDQKV 125

Query: 136 T--LDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWNEYQEKC 195
              L +SIK GD  Y   L +MASK+AYEN+  +   +   W+M+ LGF++  N++ +  
Sbjct: 126 ELGLGRSIKIGDERYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDFDQTR 185

Query: 196 STQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKALGLQ 255
           ST+  ++RD   + + I++SFRGT+PFNADDW +D D+SW+ +  +GKIHGGFMKALGL 
Sbjct: 186 STEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKALGLP 245

Query: 256 KSLGWPKQLPA--RHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAI 315
           K  GW +++ +         LAYYT+  +L E+ +Q+  S+F+++GHSLGGALAILF A+
Sbjct: 246 KE-GWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILFTAV 305

Query: 316 LAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPLD 375
           L  H +K +L+RLEGVYTFGQPRVGD  FG +M   L ++ ++Y R+VY  DMVPRLP D
Sbjct: 306 LIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRLPFD 365

Query: 376 DKALMFKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRRG 435
           DK LMFKHFG C+Y D  Y  +V EEEP KNYF++   I   I A  E+ RSF I+  +G
Sbjct: 366 DKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISCWQG 425

Query: 436 KEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGS 466
           +EY E   L   RL  +L+PG+PAH P +YVN   LG+
Sbjct: 426 REYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGN 462

[Close]

BLAST of Moc01g25400 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 419.1 bits (1076), Expect = 4.9e-117
Identity = 215/472 (45.55%), Postives = 303/472 (64.19%), Query Frame = 0


Query: 10  NKGFSTSYMLLKPEEVKFFDLGRLLFSSNLKKRKFVDSSHAREYNFWR----RFFIFLSI 69
           N  F  SY L+ P +  F DL  LLFSSNL   +F+DS       F R    R+ + L+I
Sbjct: 5   NMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAI 64

Query: 70  IILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEYLSLIG 129
            + K+L L++ P A++G  L + LN L+AN GF  ++LN++  KL+ P+ SSA Y S IG
Sbjct: 65  FLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYTSFIG 124

Query: 130 HLDSRVTLDKSIKRGDVNYFGALCMMASKVAYENQAHVIQTVNHVWKMEFLGFFNFWNEY 189
             D R+ LD+ I  G + Y   L +MASK++YE++ ++   V + WKM+ +G ++F+N +
Sbjct: 125 CSDRRIELDEKINVGSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 184

Query: 190 QEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWSSDFDISWYELQGIGKIHGGFMKA 249
           QE   TQAF+ +  + + D I++SFRGTEPF A DW +D D+SWYE++ +GK+H GF +A
Sbjct: 185 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 244

Query: 250 LGLQKSLGWPKQLPARHGPARPLAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFP 309
           LGLQK  GWPK+           AYYT+R+ L + L +++  ++++TGHSLGGALA LFP
Sbjct: 245 LGLQKD-GWPKE---NISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFP 304

Query: 310 AILAFHGDKLLLQRLEGVYTFGQPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLP 369
           AILA HG+  LL +LEG+YTFGQPRVGD  FGEFM   + ++ I Y RFVY  D+VPR+P
Sbjct: 305 AILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVP 364

Query: 370 LDDKALM-FKHFGPCIYFDWNYVAQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAW 429
            DDK L  +KH+GPC  F+  Y  +V E+ P  NYF+++  I   +    E  RSF + +
Sbjct: 365 FDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQF 424

Query: 430 RRGKEYEERVFLRMVRLFGVLLPGIPAHCPQDYVNSTRLGSTEIFASPPLTT 477
            +G EY+E   +R VR+ G++ PG   H P DYVNSTRLG   +   PP TT
Sbjct: 425 WKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGG--LVRPPPTTT 470

[Close]

BLAST of Moc01g25400 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-100
Identity = 202/497 (40.64%), Postives = 290/497 (58.35%), Query Frame = 0


Query: 10  NKGFSTSYMLLKPEEVKFFDLGRL-LFSSNLKKRKFVDSSHAREYNFW--------RRFF 69
           N G  ++Y++++P    + DL R  +      K KF++    RE++           R+ 
Sbjct: 12  NPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWV 71

Query: 70  IFLSIIILKLLRLVATPLAYLGFCLEFCLNFLSANQGFSGILLNILRLKLIIPESSSAEY 129
           I +SI++ K++RL+ TP+ + GF ++F LN  SAN GF G+LL +++ K++IPE  S  +
Sbjct: 72  IVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTF 131

Query: 130 LSLIGHLDSRVTLDK------------SIKRGDVNY-FGA-----LCMMASKVAYENQAH 189
           +S IG LD R++L K            S+  G V    G+     LC+MASK+AYEN   
Sbjct: 132 VSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKV 191

Query: 190 VIQTVNHVWKMEFLGFFNFWNEYQEKCSTQAFMMRDRTVDHDTIIISFRGTEPFNADDWS 249
           V   V+  WKM  + F + WN+YQ++ STQ F+  D+  D + I+ISFRGTEPF+ADDW 
Sbjct: 192 VENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWG 251

Query: 250 SDFDISWYELQGIGKIHGGFMKALGL---------------QKSLGWPKQLPARHGPARP 309
           +DFD SWYE+  +GK+H GF++A+GL               Q S                
Sbjct: 252 TDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVER 311

Query: 310 LAYYTLRERLTELLQQSERSRFVVTGHSLGGALAILFPAILAFHGDKLLLQRLEGVYTFG 369
            AYY +R  L  LL + E +RFVVTGHSLGGALAILFP +L  + +  +++RL GVYTFG
Sbjct: 312 SAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFG 371

Query: 370 QPRVGDAKFGEFMVRSLWQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFGPCIYFDWNYV 429
           QPR+G+ + G FM   L Q   RY+R VY  D+VPRLP DDK  ++KHFG C+++D  Y 
Sbjct: 372 QPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYN 431

Query: 430 AQVLEEEPFKNYFSVVGAILMRIQACLEIGRSFTIAWRRGKEYEERVFLRMVRLFGVLLP 465
               E+EP  N + +   IL  + A  E+ R  T+ +  G +Y+E  F  + RL G+++P
Sbjct: 432 ETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIP 491

The following BLAST results are available for this feature:

BLAST of Moc01g25400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132553.1	3.3e-288	100.00	uncharacterized protein LOC111005383 [Momordica charantia]	[more]
XP_038883943.1	2.5e-232	81.12	triacylglycerol lipase OBL1-like isoform X1 [Benincasa hispida]	[more]
XP_008440283.1	9.6e-232	81.50	PREDICTED: uncharacterized protein LOC107990286 [Cucumis melo]	[more]
KAG6595033.1	1.3e-231	81.31	Lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027056.1 Lipase, pa...	[more]
XP_023543970.1	3.7e-231	80.99	uncharacterized protein LOC111803685 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc01g25400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JFU8	2.0e-99	40.64	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
A0A1S3ZP85	3.6e-96	41.30	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	5.9e-83	39.13	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
O59952	8.2e-08	31.40	Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1	[more]
P19515	4.1e-07	30.68	Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2	[more]

BLAST of Moc01g25400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWK4	1.6e-288	100.00	uncharacterized protein LOC111005383 OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A1S3B1H5	4.7e-232	81.50	uncharacterized protein LOC107990286 OS=Cucumis melo OX=3656 GN=LOC107990286 PE=...	[more]
A0A6J1GET2	2.3e-231	81.03	uncharacterized protein LOC111453276 OS=Cucurbita moschata OX=3662 GN=LOC1114532...	[more]
A0A6J1KN75	6.7e-231	80.90	uncharacterized protein LOC111497186 OS=Cucurbita maxima OX=3661 GN=LOC111497186...	[more]
A0A0A0KFS7	3.3e-230	80.63	Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490940 PE...	[more]

BLAST of Moc01g25400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67050.1	5.2e-135	52.42	alpha/beta-Hydrolases superfamily protein	[more]
AT5G42930.1	4.0e-127	50.55	alpha/beta-Hydrolases superfamily protein	[more]
AT1G56630.1	4.4e-118	48.03	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.1	4.9e-117	45.55	triacylglycerol lipase-like 1	[more]
AT3G14360.1	1.4e-100	40.64	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 207..368 e-value: 4.9E-37 score: 127.0
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 122..411 e-value: 9.7E-47 score: 161.9
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 148..404
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 9..470
None	No IPR available	PANTHER	PTHR46086:SF17	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 9..470
None	No IPR available	CDD	cd00519	Lipase_3	coord: 148..383 e-value: 8.08148E-49 score: 165.727

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g25400.1	Moc01g25400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity