Moc01g23550 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g23550
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	IAA-amino acid hydrolase ILR1
Location	chr1: 16502313 .. 16504539 (-)
RNA-Seq Expression	Moc01g23550
Synteny	Moc01g23550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGCCGCCATGGACAGCTGCTCCACGTTGTTTTGTTGGATGGTGTTCCTGGGGATGGCCTTGAGCCGATCGGCGGCGGCGGCGGCGCGCGGCGGAGATGGGTCGGAGCTCCACTCACTGACCCGGAACTTATTGGGGTCGGCGAGGGAAGGGGAGTTCATGGAGTGGATAAAGGGAGTTAGGAGGAGAATCCATGAGTATCCGGAGCTGGGATTTGAGGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTCGACTCGCTCGGAATCAGTTACCAGTGGCCTGTGGCAGAGACTGGAGTGGTGGCTTCCATTAAAGGGGCGGCTTCGCTTTCTTCTTCCAGACCTGTTTTTGCTCTCAGAGCTGACATGGATGCCCTTCCTTTGCAGGTCCTCTCTTCTTCTGCTGCTTCTTCTATCAGACATGAAGTTAAAAAAAGTCATGCATGGAGATTATTTGCTATATTTTTTCTAAGCTATATAAAGCATGATAAATACAAAGCATGTCTTTCCAATTCCATATTTTGAGTGCAATCCAATTCACTTCAGATAAGGACTAGACTAATTTTACAACCATCTTGTTTTACGTTCTTAAAAAACAAAACAGGATGGTTATATTATAAAACAGTTTGAAAACGAAAACAAAATGATTATCAGGTGGGTCAAATCAATTCTCTCTCTTGCTTCTAAAACTGAAATAATTTTTCATACCAGAAAGGCATATCAAGGTACTCCAACTAAATTTCTGTGTTCTTCTCCTTCCTACAAAATTGAAATTCAACCTTTCTAATTGTTGTTGTCAATGGCGAGGATCACGTCAGGGAATTGAGTACAATGAGTCAGCAGTTTCAGATTCTCCAAGCTTAATATAAAGTTTATTCTCTTCATATAATTTTGAAGGAATTGGTAGAATGGGAATTCAAAAGCAAGGTAGAAGGTAAAATGCATGCCTGTGGCCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTACTACAGAGTAAAAGAGACAAACTTAAGGTAAACATTTTCGGACTTTATAATTGTAAATCTGTTTGTTTGATTCAGTTAATTGGGAAGGAGACTGACCAGGATTGTCTGAATATGTTGTTATTTGAAGGGAACTGTGAAGCTAGTTTTCCAGCCTGGAGAAGAGTGTAATGGAGCTTATAAAATGCTAAAAGATGAAGCTCTTGATGGTATAGATGGAATCTTTGCATTGCATGTTCTGCCCTCCTTACCAACCGGTGTCATAGCCTCTAGGCCCGGTCCGGTATGTGCTGGAGCTGGACACTTTTCAGCCTTGATCCGAGGAAAGGGTGGACATGCTGCAATCCCACATATGACGAAGGACCCAGTTCTGGCAACAGCTTTCATAATTCAGGCTCTTCAACAAATTGTATCCCGTGAGACAGATCCTCTTGAGGCAGGAGTAAGTTTCGCGCCCTCTAAATGATCTCCATAGTGTTATATACTATAAGAACAGAATAACATCAGATAAATTATTAACTACTCAAAAAGATTCAACATCTAGTCCAAACAACAGCTCAAAATGGTCATCGGTTCACTTGTTGAACTGGAGGGTGATGCATAGCCAAGCAACTCATAAACATGATCGTAGTTGACTAATACTCAGCAATGTAGGTGGTGACAATTGCATTTGTGGAGGGAGGGCAGGCAGAAAACATAGTTCCCGAGACCGTGAAAGTCGGGGGAACTTTTAGGAGCTTGTCTCCTGAGGGTTTCTCTTACCTTAAGGAAAGGATCAGAGAGGTATGGCATACAGTTGATGAACTTAACAAATTTCCTTATTACTTTTGAAACTGGGCAGAACTTAATTCCTCCTGAACAGGTCATCAATACACAAGCAGCAGTGCACCATTGTCATGCTGCAGTGAAGTTTATGGAGGATACACCAGTAATGGTTAACAATGTAGCATTATTTGAACATGCAAATATGGTAGGAAATAGCTTACTTGGAGAGTCCAATGTGCAACTCCTTCCATGGACCATGGGAGCTGAAGATTTTGGTTTCTTCTCCCAGAGGATTGCAGCTACCATATTTGGGATTGGGGTAAGGAATGATACTCTGAGATCAAACCGGCCATTGCACTCACCACACTTTGTCCTTGATGAGGAAGCCCTTCCCATAGGAGCAGCACTTCATGCTGCTGTGGCCATGTCATACTTGGAACATAATTCAGTTTTCAGTGATTAA

mRNA sequence

ATGACAGCCGCCATGGACAGCTGCTCCACGTTGTTTTGTTGGATGGTGTTCCTGGGGATGGCCTTGAGCCGATCGGCGGCGGCGGCGGCGCGCGGCGGAGATGGGTCGGAGCTCCACTCACTGACCCGGAACTTATTGGGGTCGGCGAGGGAAGGGGAGTTCATGGAGTGGATAAAGGGAGTTAGGAGGAGAATCCATGAGTATCCGGAGCTGGGATTTGAGGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTCGACTCGCTCGGAATCAGTTACCAGTGGCCTGTGGCAGAGACTGGAGTGGTGGCTTCCATTAAAGGGGCGGCTTCGCTTTCTTCTTCCAGACCTGTTTTTGCTCTCAGAGCTGACATGGATGCCCTTCCTTTGCAGGAATTGGTAGAATGGGAATTCAAAAGCAAGGTAGAAGGTAAAATGCATGCCTGTGGCCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTACTACAGAGTAAAAGAGACAAACTTAAGGGAACTGTGAAGCTAGTTTTCCAGCCTGGAGAAGAGTGTAATGGAGCTTATAAAATGCTAAAAGATGAAGCTCTTGATGGTATAGATGGAATCTTTGCATTGCATGTTCTGCCCTCCTTACCAACCGGTGTCATAGCCTCTAGGCCCGGTCCGGTATGTGCTGGAGCTGGACACTTTTCAGCCTTGATCCGAGGAAAGGGTGGACATGCTGCAATCCCACATATGACGAAGGACCCAGTTCTGGCAACAGCTTTCATAATTCAGGCTCTTCAACAAATTGTATCCCGTGAGACAGATCCTCTTGAGGCAGGAGTGGTGACAATTGCATTTGTGGAGGGAGGGCAGGCAGAAAACATAGTTCCCGAGACCGTGAAAGTCGGGGGAACTTTTAGGAGCTTGTCTCCTGAGGGTTTCTCTTACCTTAAGGAAAGGATCAGAGAGGTCATCAATACACAAGCAGCAGTGCACCATTGTCATGCTGCAGTGAAGTTTATGGAGGATACACCAGTAATGGTTAACAATGTAGCATTATTTGAACATGCAAATATGGTAGGAAATAGCTTACTTGGAGAGTCCAATGTGCAACTCCTTCCATGGACCATGGGAGCTGAAGATTTTGGTTTCTTCTCCCAGAGGATTGCAGCTACCATATTTGGGATTGGGGTAAGGAATGATACTCTGAGATCAAACCGGCCATTGCACTCACCACACTTTGTCCTTGATGAGGAAGCCCTTCCCATAGGAGCAGCACTTCATGCTGCTGTGGCCATGTCATACTTGGAACATAATTCAGTTTTCAGTGATTAA

Coding sequence (CDS)

ATGACAGCCGCCATGGACAGCTGCTCCACGTTGTTTTGTTGGATGGTGTTCCTGGGGATGGCCTTGAGCCGATCGGCGGCGGCGGCGGCGCGCGGCGGAGATGGGTCGGAGCTCCACTCACTGACCCGGAACTTATTGGGGTCGGCGAGGGAAGGGGAGTTCATGGAGTGGATAAAGGGAGTTAGGAGGAGAATCCATGAGTATCCGGAGCTGGGATTTGAGGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTCGACTCGCTCGGAATCAGTTACCAGTGGCCTGTGGCAGAGACTGGAGTGGTGGCTTCCATTAAAGGGGCGGCTTCGCTTTCTTCTTCCAGACCTGTTTTTGCTCTCAGAGCTGACATGGATGCCCTTCCTTTGCAGGAATTGGTAGAATGGGAATTCAAAAGCAAGGTAGAAGGTAAAATGCATGCCTGTGGCCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTACTACAGAGTAAAAGAGACAAACTTAAGGGAACTGTGAAGCTAGTTTTCCAGCCTGGAGAAGAGTGTAATGGAGCTTATAAAATGCTAAAAGATGAAGCTCTTGATGGTATAGATGGAATCTTTGCATTGCATGTTCTGCCCTCCTTACCAACCGGTGTCATAGCCTCTAGGCCCGGTCCGGTATGTGCTGGAGCTGGACACTTTTCAGCCTTGATCCGAGGAAAGGGTGGACATGCTGCAATCCCACATATGACGAAGGACCCAGTTCTGGCAACAGCTTTCATAATTCAGGCTCTTCAACAAATTGTATCCCGTGAGACAGATCCTCTTGAGGCAGGAGTGGTGACAATTGCATTTGTGGAGGGAGGGCAGGCAGAAAACATAGTTCCCGAGACCGTGAAAGTCGGGGGAACTTTTAGGAGCTTGTCTCCTGAGGGTTTCTCTTACCTTAAGGAAAGGATCAGAGAGGTCATCAATACACAAGCAGCAGTGCACCATTGTCATGCTGCAGTGAAGTTTATGGAGGATACACCAGTAATGGTTAACAATGTAGCATTATTTGAACATGCAAATATGGTAGGAAATAGCTTACTTGGAGAGTCCAATGTGCAACTCCTTCCATGGACCATGGGAGCTGAAGATTTTGGTTTCTTCTCCCAGAGGATTGCAGCTACCATATTTGGGATTGGGGTAAGGAATGATACTCTGAGATCAAACCGGCCATTGCACTCACCACACTTTGTCCTTGATGAGGAAGCCCTTCCCATAGGAGCAGCACTTCATGCTGCTGTGGCCATGTCATACTTGGAACATAATTCAGTTTTCAGTGATTAA

Protein sequence

MTAAMDSCSTLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNSVFSD

Homology

[Close]

BLAST of Moc01g23550 vs. NCBI nr
Match: XP_022132922.1 (IAA-amino acid hydrolase ILR1-like 3 [Momordica charantia])

HSP 1 Score: 877.1 bits (2265), Expect = 6.4e-251
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0


Query: 1   MTAAMDSCSTLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKG 60
           MTAAMDSCSTLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKG
Sbjct: 1   MTAAMDSCSTLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKG 60

Query: 61  VRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFA 120
           VRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFA
Sbjct: 61  VRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFA 120

Query: 121 LRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVF 180
           LRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVF
Sbjct: 121 LRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVF 180

Query: 181 QPGEECNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKG 240
           QPGEECNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKG
Sbjct: 181 QPGEECNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKG 240

Query: 241 GHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVG 300
           GHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVG
Sbjct: 241 GHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVG 300

Query: 301 GTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNS 360
           GTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNS
Sbjct: 301 GTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNS 360

Query: 361 LLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPI 420
           LLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPI
Sbjct: 361 LLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPI 420

Query: 421 GAALHAAVAMSYLEHNSVFSD 442
           GAALHAAVAMSYLEHNSVFSD
Sbjct: 421 GAALHAAVAMSYLEHNSVFSD 441

[Close]

BLAST of Moc01g23550 vs. NCBI nr
Match: XP_023543889.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 776.5 bits (2004), Expect = 1.2e-220
Identity = 389/431 (90.26%), Postives = 410/431 (95.13%), Query Frame = 0


Query: 11  LFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPE 70
           LFCWM+F G+AL R  +AA  GGDGSELHSLT+ L+G AREG+FMEWIKGVRRRIHEYPE
Sbjct: 11  LFCWMLFFGVALWR-PSAAGHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPE 70

Query: 71  LGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRADMDALPL 130
           LGFEEYRTSQLVRSELDSLGISYQWPVA+TGVVASI G ASLSSS PVFALRADMDALPL
Sbjct: 71  LGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITG-ASLSSSTPVFALRADMDALPL 130

Query: 131 QELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAY 190
           QELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSKR KLKGTVKLVFQPGEECNGAY
Sbjct: 131 QELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEECNGAY 190

Query: 191 KMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTK 250
           +ML+++ALD IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA PH TK
Sbjct: 191 QMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTK 250

Query: 251 DPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEG 310
           DPVLATAFIIQALQQIVSRETDPLEAGVVT+AFV+GGQAEN+VPETVKVGGTFRSLSPEG
Sbjct: 251 DPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEG 310

Query: 311 FSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESNVQLL 370
            SYLKERIREVIN QA VHHC+A+V+FMEDTPVMVNN ALFEHAN VGNSLLGESNVQLL
Sbjct: 311 LSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGNSLLGESNVQLL 370

Query: 371 PWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAM 430
           PWTMGAEDFGFFSQRIAATI+GIGVRN+TL S+RPLHSPHFVLDEEALPIGAALHAAVAM
Sbjct: 371 PWTMGAEDFGFFSQRIAATIYGIGVRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAM 430

Query: 431 SYLEHNSVFSD 442
           SYLEHNSVFSD
Sbjct: 431 SYLEHNSVFSD 439

[Close]

BLAST of Moc01g23550 vs. NCBI nr
Match: XP_022925994.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata] >KAG6604330.1 IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034481.1 IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 773.5 bits (1996), Expect = 1.0e-219
Identity = 387/431 (89.79%), Postives = 409/431 (94.90%), Query Frame = 0


Query: 11  LFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPE 70
           LFCWM+  G+AL R  +AA  GGDGSELHSLT+ L+G AREG+FMEWIKGVRRRIHEYPE
Sbjct: 10  LFCWMLLFGVALWR-PSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPE 69

Query: 71  LGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRADMDALPL 130
           LGFEEYRTSQLVRSELDSLGISYQWPVA+TGVVASI G ASLSSS PVFALRADMDALPL
Sbjct: 70  LGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITG-ASLSSSMPVFALRADMDALPL 129

Query: 131 QELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAY 190
           QELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSKR KLKGTVKLVFQPGEECNGAY
Sbjct: 130 QELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEECNGAY 189

Query: 191 KMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTK 250
           +ML+++ALD IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA PH TK
Sbjct: 190 QMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTK 249

Query: 251 DPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEG 310
           DPVLATAFIIQALQQIVSRETDPLEAGVVT+AFV+GGQAEN+VPETVKVGGTFRSLSPEG
Sbjct: 250 DPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEG 309

Query: 311 FSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESNVQLL 370
            SYLKERIREVIN QA VHHC+A+V+FMEDTPVMVNN ALFEHAN VGNSLLGESNVQLL
Sbjct: 310 LSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLGESNVQLL 369

Query: 371 PWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAM 430
           PWTMGAEDFGFFSQRIAATI+GIG+RN+TL S+RPLHSPHFVLDEEALPIGAALHAAVAM
Sbjct: 370 PWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAM 429

Query: 431 SYLEHNSVFSD 442
           SYLEHNSVFSD
Sbjct: 430 SYLEHNSVFSD 438

[Close]

BLAST of Moc01g23550 vs. NCBI nr
Match: XP_022979169.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita maxima])

HSP 1 Score: 767.3 bits (1980), Expect = 7.2e-218
Identity = 386/431 (89.56%), Postives = 409/431 (94.90%), Query Frame = 0


Query: 11  LFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPE 70
           LFC M+F G+AL R  +AA  GGDGS+LHSLT+ L+G AREGEFMEWIKGVRRRIHEYPE
Sbjct: 10  LFCSMLFFGVALWR-PSAAEHGGDGSDLHSLTQKLMGLAREGEFMEWIKGVRRRIHEYPE 69

Query: 71  LGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRADMDALPL 130
           LGFEEYRTSQLVRSELDSLGISYQWPVA+TGVVASI G ASLSSS PVFALRADMDALPL
Sbjct: 70  LGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITG-ASLSSSTPVFALRADMDALPL 129

Query: 131 QELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAY 190
           QELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLL+SKR KLKGTVKLVFQPGEECNGAY
Sbjct: 130 QELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLRSKRLKLKGTVKLVFQPGEECNGAY 189

Query: 191 KMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTK 250
           +ML+++ALD IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA PH TK
Sbjct: 190 QMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTK 249

Query: 251 DPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEG 310
           DPVLATAFIIQALQQIVSRETDPLEAGVVT+AFV+GGQAEN+VPETVKVGGTFRSLSPEG
Sbjct: 250 DPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEG 309

Query: 311 FSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESNVQLL 370
            SYLKERIREVIN QA VHHC+A+V+FMEDTPVMVNN ALFEHAN VGNSLLGESNVQLL
Sbjct: 310 LSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGNSLLGESNVQLL 369

Query: 371 PWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAM 430
           PWTMGAEDFGFFSQRIAATI+GIG+RN+TL S+RPLHSPHFVLDEEALPIGAALHAAVAM
Sbjct: 370 PWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAM 429

Query: 431 SYLEHNSVFSD 442
           SYLEHNSVFSD
Sbjct: 430 SYLEHNSVFSD 438

[Close]

BLAST of Moc01g23550 vs. NCBI nr
Match: XP_022962856.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata])

HSP 1 Score: 763.5 bits (1970), Expect = 1.0e-216
Identity = 381/430 (88.60%), Postives = 406/430 (94.42%), Query Frame = 0


Query: 11  LFCWMVFLGMALSR-SAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYP 70
           LFCWM+  G+AL R SA+    GGDG ELHSLTR LLG AREGEF+EWIKGVRRRIHEYP
Sbjct: 33  LFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYP 92

Query: 71  ELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGA---ASLSSSRPVFALRADMD 130
           E+GFEEYRTSQLVRSELD LGISYQWPVA+TGVVASIKGA   +S SSSRPVFALRADMD
Sbjct: 93  EVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVFALRADMD 152

Query: 131 ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEEC 190
           ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK +KLKGTVKLVFQPGEEC
Sbjct: 153 ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLVFQPGEEC 212

Query: 191 NGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIP 250
           NGAY++L+++ALD IDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGKGGHAA P
Sbjct: 213 NGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAP 272

Query: 251 HMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSL 310
           H TKDPVLATAFIIQALQQIVSRETDPLEAGVVT+AFV+GGQA+N+VPETVKVGGTFRSL
Sbjct: 273 HKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSL 332

Query: 311 SPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESN 370
           SP+G SYLKERIREVINTQA VHHC+A+V+FMEDTPVMVNNVALFEHAN VGNSLLGESN
Sbjct: 333 SPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGNSLLGESN 392

Query: 371 VQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHA 430
           VQLLPWTMGAEDFGF SQ+IAATI+GIG+RNDTLRSNRPLHSPHFVLDEEALPIGAALHA
Sbjct: 393 VQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAALHA 452

Query: 431 AVAMSYLEHN 437
           AVAMSYLEHN
Sbjct: 453 AVAMSYLEHN 462

[Close]

BLAST of Moc01g23550 vs. ExPASy Swiss-Prot
Match: P54968 (IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2)

HSP 1 Score: 499.6 bits (1285), Expect = 3.6e-140
Identity = 250/437 (57.21%), Postives = 326/437 (74.60%), Query Frame = 0


Query: 5   MDSCSTLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRR 64
           MD   + F  ++F+        ++A     GS L SL R +L SA++ EF EW++G+RR+
Sbjct: 1   MDFSGSFF--VIFVTFFFLPPLSSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRK 60

Query: 65  IHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRAD 124
           IHE PE GF+E++TSQLVR ELDSLG+ Y++PVA+TGVVA I      S S+PVF LRAD
Sbjct: 61  IHENPETGFQEFKTSQLVRDELDSLGVKYKYPVAKTGVVAWIG-----SCSKPVFGLRAD 120

Query: 125 MDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGE 184
           MDALPLQELVEWE KSKV+GKMHACGHD+HVAMLLGAA+LLQ+ +  +KGTVKLVFQPGE
Sbjct: 121 MDALPLQELVEWESKSKVDGKMHACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGE 180

Query: 185 E-CNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 244
           E   GAY+MLKDE LD +DGI ++HV PS+P+G I SRPG V AGAG F+  + G+G HA
Sbjct: 181 EGYAGAYEMLKDEILDDLDGILSVHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHA 240

Query: 245 AIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTF 304
           A PH +KDPVLA +  + ALQQIVSRE DPLEAGVVT+ ++EGG A+N++P++ K GGTF
Sbjct: 241 ATPHFSKDPVLAASSAVVALQQIVSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTF 300

Query: 305 RSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDT----PVMVNNVALFEHANMVGN 364
           RSLS +G  +++ RI+E+   QA+V+ C A V F E      PVM N+  L+EH   V  
Sbjct: 301 RSLSNDGLLFIQRRIKEISEAQASVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAE 360

Query: 365 SLLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALP 424
           +++G++N    P TMG EDF FF+Q+  A IF +GV+N+TL + +PLHSP+F +DEEALP
Sbjct: 361 AMIGKNNFHDFPVTMGGEDFSFFTQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALP 420

Query: 425 IGAALHAAVAMSYLEHN 437
           +GAALHAA+A+SYL+ +
Sbjct: 421 VGAALHAAMAVSYLDEH 430

[Close]

BLAST of Moc01g23550 vs. ExPASy Swiss-Prot
Match: Q851L5 (IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL3 PE=2 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 3.9e-134
Identity = 240/408 (58.82%), Postives = 304/408 (74.51%), Query Frame = 0


Query: 40  SLTRNLLGSAREGEFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAE 99
           +L R LL +AR  EF  W++G+RRRIH++PEL F+E+RTS LVR+ELD+LG++Y WPVA+
Sbjct: 8   TLGRELLEAARAPEFAGWLRGLRRRIHQHPELAFQEHRTSALVRAELDALGVAYVWPVAQ 67

Query: 100 TGVVASIKGAASLSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLL 159
           TGVVA++ GAA      PVF LRADMDALP+QE+VEWEFKS  +GKMHACGHD HVAMLL
Sbjct: 68  TGVVATVVGAA---GPGPVFGLRADMDALPIQEMVEWEFKSLEDGKMHACGHDVHVAMLL 127

Query: 160 GAARLLQSKRDKLKGTVKLVFQPGEE-CNGAYKMLKDEALDGIDGIFALHVLPSLPTGVI 219
           GAA+LLQS+RD   G VKLVFQP EE   G Y +L++ A+D + GIF +HV   LP GV+
Sbjct: 128 GAAKLLQSRRDHFNGKVKLVFQPAEEGYAGGYYVLEEGAVDDVQGIFGMHVDAGLPAGVV 187

Query: 220 ASRPGPVCAGAGHFSALIRGKGGHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGV 279
           ASRPGP  AG+  F+A I GKGGHAA PH   DP++A +  + +LQQIV+RETDPL+  V
Sbjct: 188 ASRPGPFLAGSARFTATINGKGGHAAAPHHAVDPIVAVSSAVLSLQQIVARETDPLQGAV 247

Query: 280 VTIAFVEGGQAENIVPETVKVGGTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFM 339
           V++  ++GG+A N++PE+V +GGT RS++ +G SYL +RIREVI  QAAV+ C AAV FM
Sbjct: 248 VSVTTIKGGEAFNVIPESVTLGGTLRSMTTDGMSYLMKRIREVIEGQAAVNRCTAAVDFM 307

Query: 340 EDT----PVMVNNVALFEHANMVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIG 399
           ED     P  VN+  ++ HA  V  S+LGE+NV+L P  MGAEDFGF++QRI A  FGIG
Sbjct: 308 EDKLPPYPATVNDEEMYAHAKAVAESMLGEANVKLSPQGMGAEDFGFYAQRIPAAFFGIG 367

Query: 400 VRND-----TLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNS 438
           V ND        +   LHSPHFV+DEEALP+GAA HAAVA+ YL  N+
Sbjct: 368 VGNDGGGMAETTTKNQLHSPHFVVDEEALPVGAAFHAAVAIEYLNKNA 412

[Close]

BLAST of Moc01g23550 vs. ExPASy Swiss-Prot
Match: Q851L6 (IAA-amino acid hydrolase ILR1-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL4 PE=2 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 2.4e-131
Identity = 234/409 (57.21%), Postives = 300/409 (73.35%), Query Frame = 0


Query: 40  SLTRNLLGSAREGEFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAE 99
           +L R LL +AR  EF  W++G+RRRIH++PEL F+E+RTS LVR+ELD+LG++Y WP+A+
Sbjct: 4   TLGRELLEAARAPEFAGWLRGLRRRIHQHPELAFQEHRTSALVRAELDALGVAYVWPIAQ 63

Query: 100 TGVVASIKGAASLSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLL 159
           TGVVA++ GAA      PVFALRADMDALP+QE+VEWEFKS  +GKMHACGHD+HVAMLL
Sbjct: 64  TGVVATVAGAA---GPGPVFALRADMDALPIQEMVEWEFKSLEDGKMHACGHDAHVAMLL 123

Query: 160 GAARLLQSKRDKLKGTVKLVFQPGEECNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIA 219
            AA+LLQS+RD   G VKLVFQP E   G Y +LK+  LD    IFA+HV   LP GV+ 
Sbjct: 124 VAAKLLQSRRDHFNGKVKLVFQPAEGGAGGYHVLKEGVLDDTQTIFAVHVATDLPAGVVG 183

Query: 220 SRPGPVCAGAGHFSALIRGKGGHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVV 279
           SRPGP  AG+  F+A I GKGGHAA PH+  DP++A +  + +LQQIV+RET+PL+  VV
Sbjct: 184 SRPGPFLAGSARFTATITGKGGHAAEPHLAVDPIVAASSAVLSLQQIVARETNPLQGAVV 243

Query: 280 TIAFVEGGQAENIVPETVKVGGTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFME 339
           ++  ++GG+A N++PE+V +GGT RS++ +G SYL  RIREVI  QAAV+ C AAV FME
Sbjct: 244 SVTTIKGGEAFNVIPESVTLGGTLRSMTTDGLSYLMNRIREVIEGQAAVNRCTAAVDFME 303

Query: 340 DT----PVMVNNVALFEHANMVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGV 399
           D     P  VN+  ++ HA  V  S+LGE+NV + P  MGAEDFGF++QRI A  FGIGV
Sbjct: 304 DKLRPYPATVNDEGMYAHAKAVAESMLGEANVTVSPMCMGAEDFGFYAQRIPAAFFGIGV 363

Query: 400 RND-------TLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNS 438
            ++          +   LHSPHFV+DEEALP+GAA HAAVA+ YL  N+
Sbjct: 364 GSNGNDGGGMAETTKNQLHSPHFVVDEEALPVGAAFHAAVAIEYLNKNA 409

[Close]

BLAST of Moc01g23550 vs. ExPASy Swiss-Prot
Match: Q8H3C8 (IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL8 PE=2 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.4e-128
Identity = 229/405 (56.54%), Postives = 299/405 (73.83%), Query Frame = 0


Query: 38  LHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPV 97
           L +L  +LL +A    F  W+ G+RRRIH+ PEL F+E RTS+LVR+ELD++G+ Y WPV
Sbjct: 37  LKALGEDLLAAAGAAGFAGWLSGLRRRIHQRPELAFQEVRTSELVRAELDAIGVPYAWPV 96

Query: 98  AETGVVASIKGAASLSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAM 157
           A TGVVA+I G A    + PV ALRADMDALPLQELV+WEFKS+ +GKMHACGHD+HV M
Sbjct: 97  ARTGVVATIDGGA---GAGPVVALRADMDALPLQELVDWEFKSQEKGKMHACGHDAHVTM 156

Query: 158 LLGAARLLQSKRDKLKGTVKLVFQPGEECN-GAYKMLKDEALDGIDGIFALHVLPSLPTG 217
           LLGAA+LLQS++D+LKGT+KLVFQP EE + GAY +L+   LD +  IF LHV+P+LP G
Sbjct: 157 LLGAAKLLQSRKDELKGTIKLVFQPAEEGHAGAYHVLESGLLDDVSVIFGLHVIPNLPVG 216

Query: 218 VIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEA 277
           V+ASRPGP  + A  F+A   GKGGHA +PH   DPV+A +  + +LQQ+VSRETDPLEA
Sbjct: 217 VVASRPGPFMSAAARFAATFTGKGGHAGVPHDAVDPVVAVSSAVLSLQQLVSRETDPLEA 276

Query: 278 GVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVK 337
            VV+I  ++GG A N++PE+  +GGTFRS++ EG +YL +RIRE+I  QA V+ C AAV 
Sbjct: 277 AVVSITILKGGDAYNVIPESASLGGTFRSMTDEGLAYLMKRIREIIEAQAGVNRCAAAVD 336

Query: 338 FMEDT----PVMVNNVALFEHANMVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIFG 397
           F+E+     P  VN+  ++ HA  V  ++LGE+NV++   +MG EDF F+++R     F 
Sbjct: 337 FLEEELRPYPATVNDDGMYGHAKAVAEAMLGEANVRVAARSMGGEDFAFYARRSPGAFFF 396

Query: 398 IGVRNDT----LRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 434
           IGV N+T      + RP+HSPHFVLDE ALP+GAALHAAVA+ YL
Sbjct: 397 IGVGNETTMGPAAAVRPVHSPHFVLDERALPVGAALHAAVAIEYL 438

[Close]

BLAST of Moc01g23550 vs. ExPASy Swiss-Prot
Match: Q8H3C9 (IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL7 PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 3.9e-126
Identity = 234/423 (55.32%), Postives = 296/423 (69.98%), Query Frame = 0


Query: 21  ALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPELGFEEYRTSQ 80
           A +  AA A  GG GS        LL +AR   F  W++G+RR IH +PEL FEE RTS+
Sbjct: 34  AAAADAAPAGGGGGGS-----GGELLSAARAPGFAAWLRGLRRSIHRHPELAFEEVRTSE 93

Query: 81  LVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRADMDALPLQELVEWEFKS 140
           LVR+ELD++G+ Y+WPVA TGVVA+I G    + +  VFALRADMDALPLQELV+WE KS
Sbjct: 94  LVRAELDAIGVPYEWPVARTGVVATIAGGDG-AGAGTVFALRADMDALPLQELVDWEHKS 153

Query: 141 KVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEE-CNGAYKMLKDEALD 200
           +  GKMHACGHD+H  MLLGAA+LLQS++D LKGTVKLVFQP EE   GA  +L++  LD
Sbjct: 154 EESGKMHACGHDAHTTMLLGAAKLLQSQKDDLKGTVKLVFQPAEEGYAGARYVLQEGVLD 213

Query: 201 GIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTKDPVLATAFI 260
            +  IF LHV P +  G + SRPGP  A +G F A I GKGGHAA PH   DP+L  +  
Sbjct: 214 DVSAIFGLHVDPRIQVGTVTSRPGPFLAASGRFLATITGKGGHAAGPHNAVDPILTASSA 273

Query: 261 IQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEGFSYLKERIR 320
           I +LQQIV+RETDPLEA V+++ F++GG A N++PE+V  GGTFRSL+ EG SYLK+RI+
Sbjct: 274 IVSLQQIVARETDPLEAAVISVTFMKGGDAYNVIPESVSFGGTFRSLTSEGLSYLKKRIK 333

Query: 321 EVINTQAAVHHCHAAVKFMEDT----PVMVNNVALFEHANMVGNSLLGESNVQLLPWTMG 380
           E++   A VH C A V FME+     P  VN+  ++ HA  V   +LGE  V++    MG
Sbjct: 334 EIVEAHATVHRCTATVDFMEEERIPYPATVNDEGMYRHARAVAVDVLGEDGVKVGTPFMG 393

Query: 381 AEDFGFFSQRIAATIFGIGVRND-TLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLE 438
           +EDF F++QR  A  F IGV N+ T+R   PLHSPHFV+DE+ LP+GAALHAAVAM YL 
Sbjct: 394 SEDFAFYAQRFPAAFFMIGVGNETTMRKVYPLHSPHFVVDEDVLPVGAALHAAVAMEYLN 450

[Close]

BLAST of Moc01g23550 vs. ExPASy TrEMBL
Match: A0A6J1BV66 (IAA-amino acid hydrolase ILR1-like 3 OS=Momordica charantia OX=3673 GN=LOC111005645 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.1e-251
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0


Query: 1   MTAAMDSCSTLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKG 60
           MTAAMDSCSTLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKG
Sbjct: 1   MTAAMDSCSTLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKG 60

Query: 61  VRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFA 120
           VRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFA
Sbjct: 61  VRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFA 120

Query: 121 LRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVF 180
           LRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVF
Sbjct: 121 LRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVF 180

Query: 181 QPGEECNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKG 240
           QPGEECNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKG
Sbjct: 181 QPGEECNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKG 240

Query: 241 GHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVG 300
           GHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVG
Sbjct: 241 GHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVG 300

Query: 301 GTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNS 360
           GTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNS
Sbjct: 301 GTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNS 360

Query: 361 LLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPI 420
           LLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPI
Sbjct: 361 LLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPI 420

Query: 421 GAALHAAVAMSYLEHNSVFSD 442
           GAALHAAVAMSYLEHNSVFSD
Sbjct: 421 GAALHAAVAMSYLEHNSVFSD 441

[Close]

BLAST of Moc01g23550 vs. ExPASy TrEMBL
Match: A0A6J1EDS0 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111433244 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 4.8e-220
Identity = 387/431 (89.79%), Postives = 409/431 (94.90%), Query Frame = 0


Query: 11  LFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPE 70
           LFCWM+  G+AL R  +AA  GGDGSELHSLT+ L+G AREG+FMEWIKGVRRRIHEYPE
Sbjct: 10  LFCWMLLFGVALWR-PSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPE 69

Query: 71  LGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRADMDALPL 130
           LGFEEYRTSQLVRSELDSLGISYQWPVA+TGVVASI G ASLSSS PVFALRADMDALPL
Sbjct: 70  LGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITG-ASLSSSMPVFALRADMDALPL 129

Query: 131 QELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAY 190
           QELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSKR KLKGTVKLVFQPGEECNGAY
Sbjct: 130 QELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEECNGAY 189

Query: 191 KMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTK 250
           +ML+++ALD IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA PH TK
Sbjct: 190 QMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTK 249

Query: 251 DPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEG 310
           DPVLATAFIIQALQQIVSRETDPLEAGVVT+AFV+GGQAEN+VPETVKVGGTFRSLSPEG
Sbjct: 250 DPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEG 309

Query: 311 FSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESNVQLL 370
            SYLKERIREVIN QA VHHC+A+V+FMEDTPVMVNN ALFEHAN VGNSLLGESNVQLL
Sbjct: 310 LSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLGESNVQLL 369

Query: 371 PWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAM 430
           PWTMGAEDFGFFSQRIAATI+GIG+RN+TL S+RPLHSPHFVLDEEALPIGAALHAAVAM
Sbjct: 370 PWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAM 429

Query: 431 SYLEHNSVFSD 442
           SYLEHNSVFSD
Sbjct: 430 SYLEHNSVFSD 438

[Close]

BLAST of Moc01g23550 vs. ExPASy TrEMBL
Match: A0A6J1IVY2 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111478889 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.5e-218
Identity = 386/431 (89.56%), Postives = 409/431 (94.90%), Query Frame = 0


Query: 11  LFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPE 70
           LFC M+F G+AL R  +AA  GGDGS+LHSLT+ L+G AREGEFMEWIKGVRRRIHEYPE
Sbjct: 10  LFCSMLFFGVALWR-PSAAEHGGDGSDLHSLTQKLMGLAREGEFMEWIKGVRRRIHEYPE 69

Query: 71  LGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRADMDALPL 130
           LGFEEYRTSQLVRSELDSLGISYQWPVA+TGVVASI G ASLSSS PVFALRADMDALPL
Sbjct: 70  LGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITG-ASLSSSTPVFALRADMDALPL 129

Query: 131 QELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAY 190
           QELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLL+SKR KLKGTVKLVFQPGEECNGAY
Sbjct: 130 QELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLRSKRLKLKGTVKLVFQPGEECNGAY 189

Query: 191 KMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTK 250
           +ML+++ALD IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA PH TK
Sbjct: 190 QMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTK 249

Query: 251 DPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEG 310
           DPVLATAFIIQALQQIVSRETDPLEAGVVT+AFV+GGQAEN+VPETVKVGGTFRSLSPEG
Sbjct: 250 DPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEG 309

Query: 311 FSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESNVQLL 370
            SYLKERIREVIN QA VHHC+A+V+FMEDTPVMVNN ALFEHAN VGNSLLGESNVQLL
Sbjct: 310 LSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGNSLLGESNVQLL 369

Query: 371 PWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAM 430
           PWTMGAEDFGFFSQRIAATI+GIG+RN+TL S+RPLHSPHFVLDEEALPIGAALHAAVAM
Sbjct: 370 PWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAM 429

Query: 431 SYLEHNSVFSD 442
           SYLEHNSVFSD
Sbjct: 430 SYLEHNSVFSD 438

[Close]

BLAST of Moc01g23550 vs. ExPASy TrEMBL
Match: A0A6J1HEG5 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111463223 PE=3 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 5.0e-217
Identity = 381/430 (88.60%), Postives = 406/430 (94.42%), Query Frame = 0


Query: 11  LFCWMVFLGMALSR-SAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYP 70
           LFCWM+  G+AL R SA+    GGDG ELHSLTR LLG AREGEF+EWIKGVRRRIHEYP
Sbjct: 33  LFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYP 92

Query: 71  ELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGA---ASLSSSRPVFALRADMD 130
           E+GFEEYRTSQLVRSELD LGISYQWPVA+TGVVASIKGA   +S SSSRPVFALRADMD
Sbjct: 93  EVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVFALRADMD 152

Query: 131 ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEEC 190
           ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK +KLKGTVKLVFQPGEEC
Sbjct: 153 ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLVFQPGEEC 212

Query: 191 NGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIP 250
           NGAY++L+++ALD IDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGKGGHAA P
Sbjct: 213 NGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAP 272

Query: 251 HMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSL 310
           H TKDPVLATAFIIQALQQIVSRETDPLEAGVVT+AFV+GGQA+N+VPETVKVGGTFRSL
Sbjct: 273 HKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSL 332

Query: 311 SPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESN 370
           SP+G SYLKERIREVINTQA VHHC+A+V+FMEDTPVMVNNVALFEHAN VGNSLLGESN
Sbjct: 333 SPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGNSLLGESN 392

Query: 371 VQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHA 430
           VQLLPWTMGAEDFGF SQ+IAATI+GIG+RNDTLRSNRPLHSPHFVLDEEALPIGAALHA
Sbjct: 393 VQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAALHA 452

Query: 431 AVAMSYLEHN 437
           AVAMSYLEHN
Sbjct: 453 AVAMSYLEHN 462

[Close]

BLAST of Moc01g23550 vs. ExPASy TrEMBL
Match: A0A0A0KIR8 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454350 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 7.5e-213
Identity = 376/429 (87.65%), Postives = 399/429 (93.01%), Query Frame = 0


Query: 13  CWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPELG 72
           CWM+  G+AL R  AA   GGDGSELHSLT++LLG ARE EFMEWIKGVRRRIHEYPELG
Sbjct: 13  CWMIVFGIALWR-PAAGENGGDGSELHSLTQSLLGLAREREFMEWIKGVRRRIHEYPELG 72

Query: 73  FEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKG-AASLSSSRPVFALRADMDALPLQ 132
           FEEY+TSQLVRSELDSLGISY+WPVA+TGVVASI+G + S SSS PVF LRADMDALPLQ
Sbjct: 73  FEEYKTSQLVRSELDSLGISYRWPVAKTGVVASIRGDSVSSSSSTPVFGLRADMDALPLQ 132

Query: 133 ELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAYK 192
           ELVEWEFKSKVEGKMHACGHDSHVAM+LGAARLLQS R+KLKGTVKLVFQP EECNGAY+
Sbjct: 133 ELVEWEFKSKVEGKMHACGHDSHVAMVLGAARLLQSIREKLKGTVKLVFQPAEECNGAYQ 192

Query: 193 MLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTKD 252
           MLKD+ALDGIDGIFALHV PSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA PH TKD
Sbjct: 193 MLKDDALDGIDGIFALHVQPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAATPHKTKD 252

Query: 253 PVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEGF 312
           PVLATAFIIQALQQIVSRETDPLEAGVVT+AFV+GGQAEN+VPETVKVGGTFRSLSPEGF
Sbjct: 253 PVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEGF 312

Query: 313 SYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESNVQLLP 372
           SYLKERIREVI+TQA VHHC+A+VKFMEDTPVMVNN ALFEH N VGNSLLGESNVQLLP
Sbjct: 313 SYLKERIREVISTQAMVHHCYASVKFMEDTPVMVNNEALFEHVNRVGNSLLGESNVQLLP 372

Query: 373 WTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMS 432
           WTMGAEDFGFFSQRI ATI+ IG  N+TL SNRP+HSPHFVLDEEALPIGAALHAAVA +
Sbjct: 373 WTMGAEDFGFFSQRIPATIYVIGTGNETLGSNRPVHSPHFVLDEEALPIGAALHAAVATT 432

Query: 433 YLEHNSVFS 441
           YLEHNSVFS
Sbjct: 433 YLEHNSVFS 440

[Close]

BLAST of Moc01g23550 vs. TAIR 10
Match: AT3G02875.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 499.6 bits (1285), Expect = 2.6e-141
Identity = 250/437 (57.21%), Postives = 326/437 (74.60%), Query Frame = 0


Query: 5   MDSCSTLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRR 64
           MD   + F  ++F+        ++A     GS L SL R +L SA++ EF EW++G+RR+
Sbjct: 1   MDFSGSFF--VIFVTFFFLPPLSSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRK 60

Query: 65  IHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRAD 124
           IHE PE GF+E++TSQLVR ELDSLG+ Y++PVA+TGVVA I      S S+PVF LRAD
Sbjct: 61  IHENPETGFQEFKTSQLVRDELDSLGVKYKYPVAKTGVVAWIG-----SCSKPVFGLRAD 120

Query: 125 MDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGE 184
           MDALPLQELVEWE KSKV+GKMHACGHD+HVAMLLGAA+LLQ+ +  +KGTVKLVFQPGE
Sbjct: 121 MDALPLQELVEWESKSKVDGKMHACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGE 180

Query: 185 E-CNGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 244
           E   GAY+MLKDE LD +DGI ++HV PS+P+G I SRPG V AGAG F+  + G+G HA
Sbjct: 181 EGYAGAYEMLKDEILDDLDGILSVHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHA 240

Query: 245 AIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTF 304
           A PH +KDPVLA +  + ALQQIVSRE DPLEAGVVT+ ++EGG A+N++P++ K GGTF
Sbjct: 241 ATPHFSKDPVLAASSAVVALQQIVSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTF 300

Query: 305 RSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDT----PVMVNNVALFEHANMVGN 364
           RSLS +G  +++ RI+E+   QA+V+ C A V F E      PVM N+  L+EH   V  
Sbjct: 301 RSLSNDGLLFIQRRIKEISEAQASVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAE 360

Query: 365 SLLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALP 424
           +++G++N    P TMG EDF FF+Q+  A IF +GV+N+TL + +PLHSP+F +DEEALP
Sbjct: 361 AMIGKNNFHDFPVTMGGEDFSFFTQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALP 420

Query: 425 IGAALHAAVAMSYLEHN 437
           +GAALHAA+A+SYL+ +
Sbjct: 421 VGAALHAAMAVSYLDEH 430

[Close]

BLAST of Moc01g23550 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.5e-104
Identity = 195/401 (48.63%), Postives = 266/401 (66.33%), Query Frame = 0


Query: 38  LHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPV 97
           L  +    L  A+  +F +W+ G+RRRIHE PELG+EE  TS+LVR+EL+ +G+SY++PV
Sbjct: 28  LSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPELGYEEVETSKLVRAELEKMGVSYKYPV 87

Query: 98  AETGVVASIKGAASLSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAM 157
           A TGVV  +      +   P  ALRADMDAL +QE+VEWE KSKV GKMHACGHD+H  M
Sbjct: 88  AVTGVVGYVG-----TGHAPFVALRADMDALAMQEMVEWEHKSKVPGKMHACGHDAHTTM 147

Query: 158 LLGAARLLQSKRDKLKGTVKLVFQPGEE-CNGAYKMLKDEALDGIDGIFALHVLPSLPTG 217
           LLGAA+LL+   ++L+GTV LVFQP EE   GA K+++   L+ +  IF LHV   L  G
Sbjct: 148 LLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVEAGVLENVSAIFGLHVTNQLALG 207

Query: 218 VIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEA 277
            ++SR GP+ AG+G F A I GKGGHAA+P  T DP+LA + +I +LQ +VSRE DPL++
Sbjct: 208 QVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPILAASNVIVSLQHLVSREADPLDS 267

Query: 278 GVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVK 337
            VVT+A  EGG A N++P++V +GGTFR+ S + F  LK+RI +VI  QA+V+ C+A V 
Sbjct: 268 QVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQLKKRIEQVITRQASVNMCNATVD 327

Query: 338 FMEDT----PVMVNNVALFEHANMVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIFG 397
           F+E+     P  VN+ AL +    V   +LG  N   +   MG+EDF F+ Q I      
Sbjct: 328 FIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFYQQAIPGHFSF 387

Query: 398 IGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 434
           +G++N         HSP+F ++EE LP GA+LHA++A  YL
Sbjct: 388 VGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATRYL 423

[Close]

BLAST of Moc01g23550 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 370.2 bits (949), Expect = 2.4e-102
Identity = 198/408 (48.53%), Postives = 272/408 (66.67%), Query Frame = 0


Query: 36  SELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQW 95
           ++L  + +N L  A+  +F +W+ G+RRRIHE PELG+EE  TS+LV++ELD +G+SY+ 
Sbjct: 26  NDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGVSYKN 85

Query: 96  PVAETGVVASIKGAASLSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHV 155
           PVA TGV+  +      +   P  ALRADMDALP+QE+VEWE KSK+ GKMHACGHD+H 
Sbjct: 86  PVAVTGVIGYVG-----TGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHT 145

Query: 156 AMLLGAARLLQSKRDKLKGTVKLVFQPGEECN-GAYKMLKDEALDGIDGIFALHVLPSLP 215
            MLLGAA+LL+  +++L+GTV LVFQP EE   GA K+++   L+ +  IF LHV   L 
Sbjct: 146 TMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLG 205

Query: 216 TGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTKDPVLATAFIIQALQQIVSRETDPL 275
            G ++SR G + AG+G F A I GKGGHAA+P    DPVLA + +I +LQ +VSRE DPL
Sbjct: 206 LGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPL 265

Query: 276 EAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEGFSYLKERIREVINTQAAVHHCHAA 335
           ++ VVT+A  EG  A N++P++V +GGTFR+L P+ F  LK+RI +VI TQA+V+ C+A 
Sbjct: 266 DSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNAT 325

Query: 336 VKFMEDT----PVMVNNVALFEHANMVGNSLLG-ESNVQLLPWTMGAEDFGFFSQRIAAT 395
           V F+ED     P  VNN  L      V   +LG E+ V+ LP  M +EDF F+ Q I   
Sbjct: 326 VDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLP-VMVSEDFAFYQQAIPGH 385

Query: 396 IFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNS 438
              +G++N +       HSP F ++EE LP GA+L A++A  YL  +S
Sbjct: 386 FSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSS 427

[Close]

BLAST of Moc01g23550 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 367.5 bits (942), Expect = 1.5e-101
Identity = 196/382 (51.31%), Postives = 254/382 (66.49%), Query Frame = 0


Query: 57  WIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSR 116
           W+K VRR IHE PEL FEEY TS+L+RSELD +GI Y++P+A+TG+ A I      S   
Sbjct: 88  WLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIRAWIG-----SGGP 147

Query: 117 PVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTV 176
           P  A+RADMDALP+QE VEWE  SKV GKMHACGHD+HV MLLGAA +L+++   LKGTV
Sbjct: 148 PFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHILKAREHLLKGTV 207

Query: 177 KLVFQPGEEC-NGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSAL 236
            L+FQP EE  NGA  M++D ALD ++ IFA+HV    PTGVI SR GP+ AG G F A+
Sbjct: 208 VLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLAGCGIFRAV 267

Query: 237 IRGKGGHAAIPHMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPE 296
           I  +    A      + +LA +  + +LQ IVSRE  PL++ VV++   +GG + ++ P+
Sbjct: 268 ITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSVTSFDGGHSLDVAPD 327

Query: 297 TVKVGGTFRSLSPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDT----PVMVNNVALF 356
           TV +GGTFR+ S   F YLK+RI+EV+  Q  V  C A V F E      P   NN A +
Sbjct: 328 TVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPPTTNNDATY 387

Query: 357 EHANMVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHF 416
            H   V   LLG+S+  L P  MGAEDF F+S+ I A  + IG+RN+ L S    HSPHF
Sbjct: 388 NHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSVHIAHSPHF 447

Query: 417 VLDEEALPIGAALHAAVAMSYL 434
           ++DE++LP+GAA+HAAVA  YL
Sbjct: 448 MIDEDSLPVGAAVHAAVAERYL 459

[Close]

BLAST of Moc01g23550 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 363.2 bits (931), Expect = 2.9e-100
Identity = 203/435 (46.67%), Postives = 286/435 (65.75%), Query Frame = 0


Query: 9   STLFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEY 68
           S  F  ++FL   LS S+ +     D S++ +    LL  A+  E  +W+  +RR+IHE 
Sbjct: 8   SLTFQLLLFL---LSVSSESPWIAEDTSQIQT---KLLEFAKSPEVFDWMVKIRRKIHEN 67

Query: 69  PELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRADMDAL 128
           PELG+EE  TS+L+RSEL+ +GI Y++PVA TGV+  I      +   P  ALRADMDAL
Sbjct: 68  PELGYEELETSKLIRSELELIGIKYRYPVAITGVIGYIG-----TGEPPFVALRADMDAL 127

Query: 129 PLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEE-CN 188
           P+QE VEWE KSK+ GKMHACGHD HV MLLGAA++L   R  L+GTV L+FQP EE  +
Sbjct: 128 PIQEGVEWEHKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLS 187

Query: 189 GAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPH 248
           GA KM ++ AL  ++ IF +H+   +P G  ASR G   AGAG F A+I GKGGHAAIP 
Sbjct: 188 GAKKMREEGALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQ 247

Query: 249 MTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLS 308
            T DPV+A + I+ +LQQ+VSRETDPL++ VVT++ V GG A N++P+++ +GGT R+ +
Sbjct: 248 HTIDPVVAASSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT 307

Query: 309 PEGFSYLKERIREVINTQAAVHHCHAAVKF----MEDTPVMVNNVALFEHANMVGNSLLG 368
             GF+ L++R++EVI  QAAVH C+A+V       E  P  VNN  L++    V   LLG
Sbjct: 308 --GFTQLQQRVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLG 367

Query: 369 -ESNVQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGA 428
            E+ V+  P  MG+EDF +F++ I      +G++++T       HSP + ++E+ LP GA
Sbjct: 368 QEAFVEAAP-VMGSEDFSYFAETIPGHFSLLGMQDET-NGYASSHSPLYRINEDVLPYGA 427

Query: 429 ALHAAVAMSYLEHNS 438
           A+HA++A+ YL+  +
Sbjct: 428 AIHASMAVQYLKEKA 427

The following BLAST results are available for this feature:

BLAST of Moc01g23550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132922.1	6.4e-251	100.00	IAA-amino acid hydrolase ILR1-like 3 [Momordica charantia]	[more]
XP_023543889.1	1.2e-220	90.26	IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022925994.1	1.0e-219	89.79	IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata] >KAG6604330.1 IAA-amin...	[more]
XP_022979169.1	7.2e-218	89.56	IAA-amino acid hydrolase ILR1-like 3 [Cucurbita maxima]	[more]
XP_022962856.1	1.0e-216	88.60	IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata]	[more]

BLAST of Moc01g23550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P54968	3.6e-140	57.21	IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2	[more]
Q851L5	3.9e-134	58.82	IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
Q851L6	2.4e-131	57.21	IAA-amino acid hydrolase ILR1-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
Q8H3C8	1.4e-128	56.54	IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
Q8H3C9	3.9e-126	55.32	IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]

BLAST of Moc01g23550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BV66	3.1e-251	100.00	IAA-amino acid hydrolase ILR1-like 3 OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A6J1EDS0	4.8e-220	89.79	IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC1114332...	[more]
A0A6J1IVY2	3.5e-218	89.56	IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111478889...	[more]
A0A6J1HEG5	5.0e-217	88.60	IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC1114632...	[more]
A0A0A0KIR8	7.5e-213	87.65	M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454350 P...	[more]

BLAST of Moc01g23550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02875.1	2.6e-141	57.21	Peptidase M20/M25/M40 family protein	[more]
AT1G51760.1	2.5e-104	48.63	peptidase M20/M25/M40 family protein	[more]
AT1G51780.1	2.4e-102	48.53	IAA-leucine resistant (ILR)-like gene 5	[more]
AT1G44350.1	1.5e-101	51.31	IAA-leucine resistant (ILR)-like gene 6	[more]
AT5G56660.1	2.9e-100	46.67	IAA-leucine resistant (ILR)-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017439	Amidohydrolase	TIGRFAM	TIGR01891	TIGR01891	coord: 60..421 e-value: 1.4E-113 score: 377.7
IPR017439	Amidohydrolase	PIRSF	PIRSF005962	Amidohydrol_AmhX	coord: 47..437 e-value: 1.6E-116 score: 387.3
IPR017439	Amidohydrolase	PANTHER	PTHR11014	PEPTIDASE M20 FAMILY MEMBER	coord: 13..436
IPR002933	Peptidase M20	PFAM	PF01546	Peptidase_M20	coord: 120..429 e-value: 1.2E-31 score: 110.6
IPR011650	Peptidase M20, dimerisation domain	PFAM	PF07687	M20_dimer	coord: 236..327 e-value: 1.3E-9 score: 37.9
None	No IPR available	GENE3D	3.40.630.10	Zn peptidases	coord: 61..418 e-value: 1.4E-131 score: 440.6
None	No IPR available	GENE3D	3.30.70.360		coord: 227..341 e-value: 1.4E-131 score: 440.6
None	No IPR available	PANTHER	PTHR11014:SF108	IAA-AMINO ACID HYDROLASE ILR1	coord: 13..436
None	No IPR available	SUPERFAMILY	53187	Zn-dependent exopeptidases	coord: 53..435
IPR044757	IAA-amino acid hydrolase ILR1-like	CDD	cd08017	M20_IAA_Hyd	coord: 58..433 e-value: 0.0 score: 584.667
IPR036264	Bacterial exopeptidase dimerisation domain	SUPERFAMILY	55031	Bacterial exopeptidase dimerisation domain	coord: 227..344

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g23550.1	Moc01g23550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009850	auxin metabolic process
molecular_function	GO:0010179	IAA-Ala conjugate hydrolase activity
molecular_function	GO:0016787	hydrolase activity