Moc01g22220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g22220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Isocitrate dehydrogenase [NAD] subunit, mitochondrial
Location	chr1: 15453778 .. 15457031 (+)
RNA-Seq Expression	Moc01g22220
Synteny	Moc01g22220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCGCAGCTCCTCAGACGCGCCCTCGGAAGCCGCTCGGGTCAGATCTTCTCCCTTTCATCTGACCTTAGCTCCTCCGCTCCCGTCCCCTTGAGGGCTTTCTCTTCCGCCGCTTCTCCGATCCGAGCTACTCTCTTCCCCGGCGATGGTATTGGTCCCGAAATTGCCGAGTCCGTCAAACAGGTATTGCTTTTTCTCCATGATCGCAATTCTGTTTCAGTACGATAATTTTCCCGTTTTCTGTACCTAGTGGCAACTTCTCTGGTAATTGCTCCAGAAAAATTGATCTGAATTTGTTATTTTAATGTTTAAAGGTATGTGGGGTATTTTTCGAGTTCTCTTTTATGAGAATTTTCTTATTTGGTTCATGTCTTTGGGTTTATGTTTCTGTTATTAGATGGTGATTCAAGAGAACTACTGTCTTTTTTTTGTAGTGTCAAATCCACTTTAGAATTGCAACTCATTTTTAATACAGAAAAGAAGTAAATTTCATGCGTCTTGTGTGATAAATATACCAAGTGAGATACTTCTTGTTTGATTTACTAAGTGATGTTCATGGTATTGTGTAATTGATAAATTTCATTCTTAACCTATACAGGTATTCAATACTGCTGAAGTGCCTATCGAATGGGAAGAGCACTACGTCAGTGACCAAATAGATCCTCGAACTCAAAGTTTTCTCACATGGGAAAGCTTAGAATCAGTGAGACGTAATCGTGTGGGCTTGAAAGGACCCATGGCCACACCAATTGGAAAAGGCCATCGTTCTTTGAACCTTACCTTAAGGAAGGAACTTAATCTGTATGCTAATGTAAGGCCTTGTTACAGCCTGCCTGGTTATAAAACTCGATATGATAATGTAAATCTTATCACTATCCGTGAGAACACAGAAGGGGAGTACAGTGGACTTGAACATCAAGTAAGTATCTATAATTTCTATTGGATTGTGGAGAGCAATCTCAACGTGGTTCATGTGTACAAATTATTGCGTTCTAGGTGGTGAGAGGTGTGGTTGAAAGTCTTAAGATCATTACCCGTCAAGCAAGTTTAAGGGTGGCAGAATATGCTTTTCACTATGCCAAGACTCATGGAAGAGAAAGAGTCTCCGCGATACACAAAGCCAACATTATGCAGAAAACTGATGGTCTTTTTCTTAAGGTGTGACTAAATTTCAAAATTATTTACTATCCCCTCATCCTCACTTTTCATCCAGGTGTTCTCATCTGAAATTTTCTTTGTGTCTTCACTTTCAGTGTTGCCGTGAAGTTGCAGAGAAGTATCCTGAGATAAAGTACGAGGAAGTTGTCATTGATAACTGCTGTATGATGGTATGGATTAATTTCTTTTCAAGAATCACAGAACCCATAAAAAGTTTAATTCTGCATTGGAGGTGACGTGGAGGTCTGAGAGGGTCAATCTAGAATATCTAGTAGATTCTTGAGAAAGTATTTCTGATCATTACTTGATTTGATTATGAAATTTGGGATCATCAATGGGTTTCTGTAGAACGGCAGCGAAATTTGACTTTTGTTATTCAAGTGCAAGTAAGGTGGAACTGGTCTTCATTATGTATTGCCTTTGTGCAGCTTGTGAAGAATCCATCTCTCTTTGATGTATTGGTGATGCCTAATCTTTATGGTGACATTATCAGTGATCTCTGTGCTGGGCTGATTGGAGGATTGGGCTTAACGCCAAGGTATTTACGCCTCATTTTCATTTCTATTGGTAAGCTTAAATATTATCTGACATCCAAAAAAATAATATTATGCAGCTGTAATATTGGTGAGGGAGGTATTGCTCTTGCTGAGGCTGTGCATGGTTCAGCACCAGATATTGCTGGAAAGGTCAGGCTTCATAACTTTAAGATCATTGACTGCACCTTCATTTATTTCTTTTGTTTAATCATATATCCCTCTTGAATGCCTCATTGTTCTGTATGTTGCTTGTATTTATTGCCGGGGCTTTTATCAGCTTGCTGTGTTTTTTCACTAAGCAACTCATGGTTGGTCCTAAACTATCTCTAGCTGAACTTTAACTACCATCATAGGGTTTCGTGGTGTCAATTTAGTTCTTGCACTAATAATTTATTTCAAATTCCTCCTTTCGATCAATAATCTTGACAATTGTTAACATGTCAACCTTGGACCTCTGTTTTGTGACTAATAGGGGCATTTATGGCATGGAAATGCTTGATATTCCTGGGAATAAAATTGATGAGAATGAAATTTATGAAATTAAATGCTCGAGAGAGATAGACATGTTTAAATATAGCATTCTTAAGATAGAAAATCTGCACTATTGGATCAATGTATTTGATCTGTAGAATATGTTAATCATAAGTTGTACATTTTCAAACATAAAAATAAATTTGTGTCTGCAAGTTAGTATTATCAGTAGTAAATGATATTAAAATTGTATTCAATTATTTAAATTAAAATTAGAAGCTAAAAGATTAAGTTTTAAAAAATGACCTTGGTTGTGGGATCACATGGAAGTTAAATAGTAACAATCACACCCTTCTTTTCATAGAAAGCACTATTTTTTATGCAGAAATACAGGAATAAACATTGTAATATAGAAAGTGTATGTAGAGAGAAAGAATGGTGAGACTGTAGAGATAAATAGGAAAAAGTTTGTAGAAAAAATGAAGTGCAGAGAGAGGGAGTAGCAAGAGGAAATGTTATATGTTTCTGCGAAGAATCTCTTTTTAGGGAAAAGGGCTAAGATTTCTCACACCGTTTTTGAGAAAAATAGGAACTAAAAAAATATATTATCAAACAAATATGTGAAACAGAAAGAATAGAACATTTTCAAACAACACTTTTGAAGCCTTTTCTCCTTTTTTTTTTTTTTGGTCAAAAACATGAGTCCAATTGACTCAAATCCAAATAGGGCTTCGTACCACACATTCTTGCAGAAGGGCATACAGTAAAATGTAATTGCTTAGGTAGAATATATATCTTTTGCAAACACGCGGTGAAGAGTCGAAGTAGTAAATGCCCCTTCCTATTTGAAAGTGAATTTTCATGTATCTGTATGCTCTGGTTAAACTTGCACAGAATTTGGCAAATCCAACTGCATTGCTTTTGAGTTCTGTCACAATGCTTCGCCATTTGGAGCTCCATGACAAAGCTGATCGGATTCAAGATGCTATTCTCAAGACCATTGCGGAAGGGAAGTACCGAACAGCTGATCTCGGCGGTTCATCATCAACTACCGTGTTCACGAACGCAATTTGCGACCATCTTTGA

mRNA sequence

ATGGCTTCGCAGCTCCTCAGACGCGCCCTCGGAAGCCGCTCGGGTCAGATCTTCTCCCTTTCATCTGACCTTAGCTCCTCCGCTCCCGTCCCCTTGAGGGCTTTCTCTTCCGCCGCTTCTCCGATCCGAGCTACTCTCTTCCCCGGCGATGGTATTGGTCCCGAAATTGCCGAGTCCGTCAAACAGGTATTCAATACTGCTGAAGTGCCTATCGAATGGGAAGAGCACTACGTCAGTGACCAAATAGATCCTCGAACTCAAAGTTTTCTCACATGGGAAAGCTTAGAATCAGTGAGACGTAATCGTGTGGGCTTGAAAGGACCCATGGCCACACCAATTGGAAAAGGCCATCGTTCTTTGAACCTTACCTTAAGGAAGGAACTTAATCTGTATGCTAATGTAAGGCCTTGTTACAGCCTGCCTGGTTATAAAACTCGATATGATAATGTAAATCTTATCACTATCCGTGAGAACACAGAAGGGGAGTACAGTGGACTTGAACATCAAGTGGTGAGAGGTGTGGTTGAAAGTCTTAAGATCATTACCCGTCAAGCAAGTTTAAGGGTGGCAGAATATGCTTTTCACTATGCCAAGACTCATGGAAGAGAAAGAGTCTCCGCGATACACAAAGCCAACATTATGCAGAAAACTGATGGTCTTTTTCTTAAGTGTTGCCGTGAAGTTGCAGAGAAGTATCCTGAGATAAAGTACGAGGAAGTTGTCATTGATAACTGCTGTATGATGCTTGTGAAGAATCCATCTCTCTTTGATGTATTGGTGATGCCTAATCTTTATGGTGACATTATCAGTGATCTCTGTGCTGGGCTGATTGGAGGATTGGGCTTAACGCCAAGCTGTAATATTGGTGAGGGAGGTATTGCTCTTGCTGAGGCTGTGCATGGTTCAGCACCAGATATTGCTGGAAAGAATTTGGCAAATCCAACTGCATTGCTTTTGAGTTCTGTCACAATGCTTCGCCATTTGGAGCTCCATGACAAAGCTGATCGGATTCAAGATGCTATTCTCAAGACCATTGCGGAAGGGAAGTACCGAACAGCTGATCTCGGCGGTTCATCATCAACTACCGTGTTCACGAACGCAATTTGCGACCATCTTTGA

Coding sequence (CDS)

ATGGCTTCGCAGCTCCTCAGACGCGCCCTCGGAAGCCGCTCGGGTCAGATCTTCTCCCTTTCATCTGACCTTAGCTCCTCCGCTCCCGTCCCCTTGAGGGCTTTCTCTTCCGCCGCTTCTCCGATCCGAGCTACTCTCTTCCCCGGCGATGGTATTGGTCCCGAAATTGCCGAGTCCGTCAAACAGGTATTCAATACTGCTGAAGTGCCTATCGAATGGGAAGAGCACTACGTCAGTGACCAAATAGATCCTCGAACTCAAAGTTTTCTCACATGGGAAAGCTTAGAATCAGTGAGACGTAATCGTGTGGGCTTGAAAGGACCCATGGCCACACCAATTGGAAAAGGCCATCGTTCTTTGAACCTTACCTTAAGGAAGGAACTTAATCTGTATGCTAATGTAAGGCCTTGTTACAGCCTGCCTGGTTATAAAACTCGATATGATAATGTAAATCTTATCACTATCCGTGAGAACACAGAAGGGGAGTACAGTGGACTTGAACATCAAGTGGTGAGAGGTGTGGTTGAAAGTCTTAAGATCATTACCCGTCAAGCAAGTTTAAGGGTGGCAGAATATGCTTTTCACTATGCCAAGACTCATGGAAGAGAAAGAGTCTCCGCGATACACAAAGCCAACATTATGCAGAAAACTGATGGTCTTTTTCTTAAGTGTTGCCGTGAAGTTGCAGAGAAGTATCCTGAGATAAAGTACGAGGAAGTTGTCATTGATAACTGCTGTATGATGCTTGTGAAGAATCCATCTCTCTTTGATGTATTGGTGATGCCTAATCTTTATGGTGACATTATCAGTGATCTCTGTGCTGGGCTGATTGGAGGATTGGGCTTAACGCCAAGCTGTAATATTGGTGAGGGAGGTATTGCTCTTGCTGAGGCTGTGCATGGTTCAGCACCAGATATTGCTGGAAAGAATTTGGCAAATCCAACTGCATTGCTTTTGAGTTCTGTCACAATGCTTCGCCATTTGGAGCTCCATGACAAAGCTGATCGGATTCAAGATGCTATTCTCAAGACCATTGCGGAAGGGAAGTACCGAACAGCTGATCTCGGCGGTTCATCATCAACTACCGTGTTCACGAACGCAATTTGCGACCATCTTTGA

Protein sequence

MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESVKQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSSTTVFTNAICDHL

Homology

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BLAST of Moc01g22220 vs. NCBI nr
Match: XP_022143811.1 (isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial [Momordica charantia])

HSP 1 Score: 733.8 bits (1893), Expect = 7.4e-208
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV
Sbjct: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           TTVFTNAICDHL
Sbjct: 361 TTVFTNAICDHL 372

[Close]

BLAST of Moc01g22220 vs. NCBI nr
Match: XP_022925844.1 (isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial-like [Cucurbita moschata] >KAG7034566.1 Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 709.9 bits (1831), Expect = 1.1e-200
Identity = 357/372 (95.97%), Postives = 366/372 (98.39%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MA QLLRRALG++SGQIFSLSS+LSSS+P+P+RAFSSAASPIRATLFPGDGIGPEIAESV
Sbjct: 1   MALQLLRRALGNQSGQIFSLSSNLSSSSPLPVRAFSSAASPIRATLFPGDGIGPEIAESV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF TA+VPIEWEEHYVSDQIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFRTADVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAK HGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKAHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDA+L TIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAVLNTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           TT FTNAICDH+
Sbjct: 361 TTEFTNAICDHI 372

[Close]

BLAST of Moc01g22220 vs. NCBI nr
Match: XP_022977759.1 (isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 708.0 bits (1826), Expect = 4.3e-200
Identity = 356/372 (95.70%), Postives = 365/372 (98.12%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MA QLLRRALG++SGQIFSLSS+LSSS+P+P+R FSSAASPIRATLFPGDGIGPEIAESV
Sbjct: 1   MALQLLRRALGNQSGQIFSLSSNLSSSSPLPVRPFSSAASPIRATLFPGDGIGPEIAESV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF TA+VPIEWEEHYVSDQIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFRTADVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAK HGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKAHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDA+L TIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAVLNTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           TT FTNAICDH+
Sbjct: 361 TTEFTNAICDHI 372

[Close]

BLAST of Moc01g22220 vs. NCBI nr
Match: XP_023518600.1 (isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 706.1 bits (1821), Expect = 1.7e-199
Identity = 355/372 (95.43%), Postives = 366/372 (98.39%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MA QLLRRALG++SGQIFSLSS+LSSS+P+P+RAFSSAASPIRATLFPGDGIGPEIA+SV
Sbjct: 1   MALQLLRRALGNQSGQIFSLSSNLSSSSPLPVRAFSSAASPIRATLFPGDGIGPEIADSV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF TAEVPIEWEEHYV DQIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFTTAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNV+LITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAK HGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKAHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHL+LHDKADRIQDA+LKTIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLKLHDKADRIQDAVLKTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           T+ FTNAICDHL
Sbjct: 361 TSEFTNAICDHL 372

[Close]

BLAST of Moc01g22220 vs. NCBI nr
Match: XP_023544050.1 (isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 705.7 bits (1820), Expect = 2.2e-199
Identity = 355/372 (95.43%), Postives = 365/372 (98.12%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MA QLLRRALG++SGQIFSLSS+LSSS+P+ +RAFSSAASPIRATLFPGDGIGPEIAESV
Sbjct: 1   MALQLLRRALGNQSGQIFSLSSNLSSSSPLSVRAFSSAASPIRATLFPGDGIGPEIAESV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF TA+VPIEWEEHYVSDQIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFRTADVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAK HGR+RVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKAHGRDRVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDA+L TIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAVLNTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           TT FTNAICDH+
Sbjct: 361 TTEFTNAICDHI 372

[Close]

BLAST of Moc01g22220 vs. ExPASy Swiss-Prot
Match: Q945K7 (Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH5 PE=1 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.0e-179
Identity = 316/372 (84.95%), Postives = 339/372 (91.13%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MA+ L RR +G+RS QI    +  S +A    RAF S+ +PI ATLFPGDGIGPEIAESV
Sbjct: 3   MAANLARRLIGNRSTQILGAVNSSSGAASSVARAFCSSTTPITATLFPGDGIGPEIAESV 62

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           K+VF TA VPIEWEEHYV  +IDPRTQSFLTWESLESVRRN+VGLKGPMATPIGKGHRSL
Sbjct: 63  KKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGPMATPIGKGHRSL 122

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 123 NLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVRGVVESLKI 182

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAF YAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEI YEEV
Sbjct: 183 ITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEITYEEV 242

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNP+LFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE G+ALAEAVH
Sbjct: 243 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEDGVALAEAVH 302

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLS V MLRHL+ +++A++I  AI+ TIAEGKYRTADLGGSS+
Sbjct: 303 GSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQAEQIHSAIINTIAEGKYRTADLGGSST 362

Query: 361 TTVFTNAICDHL 373
           TT FT AICDHL
Sbjct: 363 TTEFTKAICDHL 374

[Close]

BLAST of Moc01g22220 vs. ExPASy Swiss-Prot
Match: P29696 (3-isopropylmalate dehydrogenase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.4e-169
Identity = 305/351 (86.89%), Postives = 321/351 (91.45%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASP--IRATLFPGDGIGPEIAE 60
           MA Q+ +R L  R+  +       +SS     R F+S ++   IRATLFPGDGIGPEIAE
Sbjct: 1   MALQIAKRLLRCRADSV-------ASSVRFFDRTFTSESNSNLIRATLFPGDGIGPEIAE 60

Query: 61  SVKQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHR 120
           SV+Q+F  AEVPIEWEEHYV  ++DPRT SFLTWESLESVRRN+VGLKGPMATPIGKGHR
Sbjct: 61  SVRQIFKVAEVPIEWEEHYVGTEVDPRTNSFLTWESLESVRRNKVGLKGPMATPIGKGHR 120

Query: 121 SLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESL 180
           SLNLTLRKELNLYANVRPCYSLPGYKTRYD+VNLITIRENTEGEYSGLEHQVVRGVVESL
Sbjct: 121 SLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRGVVESL 180

Query: 181 KIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYE 240
           KIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYE
Sbjct: 181 KIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYE 240

Query: 241 EVVIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEA 300
           EVVIDNCCMMLVKNP+LFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEA
Sbjct: 241 EVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEA 300

Query: 301 VHGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGK 350
           VHGSAPDIAGKNLANPTALLLSSV+MLRHLELHDKADRIQDAILKTIA GK
Sbjct: 301 VHGSAPDIAGKNLANPTALLLSSVSMLRHLELHDKADRIQDAILKTIAGGK 344

[Close]

BLAST of Moc01g22220 vs. ExPASy Swiss-Prot
Match: Q8LG77 (Isocitrate dehydrogenase [NAD] catalytic subunit 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH6 PE=1 SV=2)

HSP 1 Score: 586.3 bits (1510), Expect = 2.5e-166
Identity = 294/372 (79.03%), Postives = 330/372 (88.71%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           M + L RR +G+ S QI   SS  S       RAF S+++PI+ATLFPGDGIGPEIAESV
Sbjct: 3   MTAFLARRLIGNGSSQILGTSSSSSGPFISVSRAFFSSSTPIKATLFPGDGIGPEIAESV 62

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF  A+V I+W+E +V  ++DPRT SFLTW++L+SV +N+VGLKGPMATPIGKGHRSL
Sbjct: 63  KQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKNKVGLKGPMATPIGKGHRSL 122

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVV+GVVESLKI
Sbjct: 123 NLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVKGVVESLKI 182

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITR+AS+RVAEYAF YAKTHGR++VSAIHKANIMQKTDGLFL+CC EVA KYPEI YE+V
Sbjct: 183 ITRKASMRVAEYAFLYAKTHGRKKVSAIHKANIMQKTDGLFLQCCDEVAAKYPEIYYEKV 242

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNP+LFDVLVMPNLYGDIISDLCAGL+GGLGLTPS NIGE GIALAEAVH
Sbjct: 243 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSMNIGEDGIALAEAVH 302

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAG NLANPTALLLS V MLRHL+L+ +A++I  AI+ TIAEGKYRTADLGGSS+
Sbjct: 303 GSAPDIAGMNLANPTALLLSGVMMLRHLKLNKQAEQIHSAIINTIAEGKYRTADLGGSST 362

Query: 361 TTVFTNAICDHL 373
           TT FT AICDHL
Sbjct: 363 TTDFTKAICDHL 374

[Close]

BLAST of Moc01g22220 vs. ExPASy Swiss-Prot
Match: Q9VWH4 (Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Idh3a PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.5e-118
Identity = 208/347 (59.94%), Postives = 273/347 (78.67%), Query Frame = 0


Query: 26  SSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESVKQVFNTAEVPIEWEEHYVSDQIDPR 85
           ++ P   R++SS     + TL PGDGIGPEI+ +V+++F  A VPIEWE   V+    P 
Sbjct: 34  NATPAASRSYSSGTK--KVTLIPGDGIGPEISAAVQKIFTAANVPIEWEAVDVTPVRGPD 93

Query: 86  TQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKT 145
            +  +   +++SV  N++GLKGP+ TP+GKGHRSLNL LRKE NLYANVRPC SL GYKT
Sbjct: 94  GKFGIPQAAIDSVNTNKIGLKGPLMTPVGKGHRSLNLALRKEFNLYANVRPCRSLEGYKT 153

Query: 146 RYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERV 205
            YD+V+++TIRENTEGEYSG+EH++V GVV+S+K+IT +AS RVAEYAF YAK + R++V
Sbjct: 154 LYDDVDVVTIRENTEGEYSGIEHEIVDGVVQSIKLITEEASKRVAEYAFQYAKNNNRKKV 213

Query: 206 SAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPSLFDVLVMPNLY 265
           + +HKANIM+ +DGLFL+C R++A+K+PEI++EE  +D  C+ +V+NP  +DVLVMPNLY
Sbjct: 214 TVVHKANIMRMSDGLFLRCVRDMAQKFPEIQFEEKYLDTVCLNMVQNPGKYDVLVMPNLY 273

Query: 266 GDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTML 325
           GDI+SD+CAGL+GGLGLTPS N+G  G AL E+VHG+APDIAGK+LANPTALLLS+V ML
Sbjct: 274 GDILSDMCAGLVGGLGLTPSGNMGLNG-ALFESVHGTAPDIAGKDLANPTALLLSAVMML 333

Query: 326 RHLELHDKADRIQDAILKTIAEGKYRTADLGGSSSTTVFTNAICDHL 373
           RH+EL+  AD+I+ A  +TI EGKY T DLGG +  + FTN IC  L
Sbjct: 334 RHMELNTYADKIERAAFETIKEGKYLTGDLGGRAKCSEFTNEICAKL 377

[Close]

BLAST of Moc01g22220 vs. ExPASy Swiss-Prot
Match: Q55BI2 (Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=idhA PE=3 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 7.4e-118
Identity = 207/338 (61.24%), Postives = 270/338 (79.88%), Query Frame = 0


Query: 32  LRAFSSAASPI-RATLFPGDGIGPEIAESVKQVFNTAEVPIEWEEHYVSDQIDPRTQSFL 91
           LR +SS+ S I + TL PGDGIGPEI+ESVK+VF+  + PIEWE   V         + +
Sbjct: 13  LRNYSSSTSKIQKVTLIPGDGIGPEISESVKRVFSAVKAPIEWETVVVD------ANTGI 72

Query: 92  TWESLESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNV 151
           + E +ES+ +N++GLKGP++TPIG GH+SLNL LRK  NLYAN+RPC S+PG+KTRY+NV
Sbjct: 73  SKEVIESISKNKIGLKGPISTPIGTGHQSLNLGLRKTFNLYANIRPCLSIPGHKTRYNNV 132

Query: 152 NLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHK 211
           N + +RENTEGEYSG+E+Q V+GV +S+KIIT++AS R+A YAF YA  +GR++V+ IHK
Sbjct: 133 NTVVVRENTEGEYSGIENQPVKGVAQSIKIITKEASTRIAHYAFQYALANGRKKVTCIHK 192

Query: 212 ANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPSLFDVLVMPNLYGDIIS 271
           ANIM+++DGLF+K CREV+ +YP IKYEE+ IDN CM LV +P+  DV+V+PNLYGDI+S
Sbjct: 193 ANIMKQSDGLFVKSCREVSTRYPSIKYEELTIDNNCMQLVLDPNQMDVMVLPNLYGDIVS 252

Query: 272 DLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHLEL 331
           DLCAGLIGGLGLTPS NIGE G A+ EAVHG+APDIAGKN ANPTAL+LSS+ MLRHL  
Sbjct: 253 DLCAGLIGGLGLTPSGNIGENGSAIFEAVHGTAPDIAGKNKANPTALILSSIMMLRHLGH 312

Query: 332 HDKADRIQDAILKTIAEGKYRTADLGGSSSTTVFTNAI 369
             +A  I++A+L T+ EGK +T DLGG+SS + +T+ +
Sbjct: 313 FHEASIIENAVLNTLTEGKVKTGDLGGNSSCSEYTDEL 344

[Close]

BLAST of Moc01g22220 vs. ExPASy TrEMBL
Match: A0A6J1CRF5 (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111013632 PE=3 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 3.6e-208
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV
Sbjct: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           TTVFTNAICDHL
Sbjct: 361 TTVFTNAICDHL 372

[Close]

BLAST of Moc01g22220 vs. ExPASy TrEMBL
Match: A0A6J1EDB6 (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111433132 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.5e-201
Identity = 357/372 (95.97%), Postives = 366/372 (98.39%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MA QLLRRALG++SGQIFSLSS+LSSS+P+P+RAFSSAASPIRATLFPGDGIGPEIAESV
Sbjct: 1   MALQLLRRALGNQSGQIFSLSSNLSSSSPLPVRAFSSAASPIRATLFPGDGIGPEIAESV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF TA+VPIEWEEHYVSDQIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFRTADVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAK HGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKAHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDA+L TIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAVLNTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           TT FTNAICDH+
Sbjct: 361 TTEFTNAICDHI 372

[Close]

BLAST of Moc01g22220 vs. ExPASy TrEMBL
Match: A0A6J1ISA6 (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111477965 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.1e-200
Identity = 356/372 (95.70%), Postives = 365/372 (98.12%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MA QLLRRALG++SGQIFSLSS+LSSS+P+P+R FSSAASPIRATLFPGDGIGPEIAESV
Sbjct: 1   MALQLLRRALGNQSGQIFSLSSNLSSSSPLPVRPFSSAASPIRATLFPGDGIGPEIAESV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF TA+VPIEWEEHYVSDQIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFRTADVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAK HGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKAHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDA+L TIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAVLNTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           TT FTNAICDH+
Sbjct: 361 TTEFTNAICDHI 372

[Close]

BLAST of Moc01g22220 vs. ExPASy TrEMBL
Match: A0A6J1IAG8 (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111471141 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.8e-199
Identity = 354/372 (95.16%), Postives = 364/372 (97.85%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MAS LLRR LGSRSGQIFSLSS+L+S++P+P RAFSSAASPIRATLFPGDGIGPEIA+SV
Sbjct: 1   MASHLLRRTLGSRSGQIFSLSSNLTSASPLPFRAFSSAASPIRATLFPGDGIGPEIADSV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF TAEVPIEWEEHYV DQIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFTTAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNV+LITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAK HGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKAHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHL+LHDKADRIQDA+LKTIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLKLHDKADRIQDAVLKTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           T+ FTNAICDHL
Sbjct: 361 TSEFTNAICDHL 372

[Close]

BLAST of Moc01g22220 vs. ExPASy TrEMBL
Match: A0A6J1HE33 (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111463345 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.8e-199
Identity = 354/372 (95.16%), Postives = 364/372 (97.85%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MAS LLRR LGSRSGQIFSLSS+L+S++P+P RAFSSAASPIRATLFPGDGIGPEIA+SV
Sbjct: 1   MASHLLRRTLGSRSGQIFSLSSNLTSASPLPFRAFSSAASPIRATLFPGDGIGPEIADSV 60

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF TAEVPIEWEEHYV DQIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFTTAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNV+LITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAK HGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKAHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLSSVTMLRHL+LHDKADRIQDA+LKTIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLKLHDKADRIQDAVLKTIAEGKYRTADLGGSSS 360

Query: 361 TTVFTNAICDHL 373
           T+ FTNAICDHL
Sbjct: 361 TSEFTNAICDHL 372

[Close]

BLAST of Moc01g22220 vs. TAIR 10
Match: AT5G03290.1 (isocitrate dehydrogenase V )

HSP 1 Score: 629.8 bits (1623), Expect = 1.4e-180
Identity = 316/372 (84.95%), Postives = 339/372 (91.13%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           MA+ L RR +G+RS QI    +  S +A    RAF S+ +PI ATLFPGDGIGPEIAESV
Sbjct: 3   MAANLARRLIGNRSTQILGAVNSSSGAASSVARAFCSSTTPITATLFPGDGIGPEIAESV 62

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           K+VF TA VPIEWEEHYV  +IDPRTQSFLTWESLESVRRN+VGLKGPMATPIGKGHRSL
Sbjct: 63  KKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGPMATPIGKGHRSL 122

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 123 NLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVRGVVESLKI 182

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITRQASLRVAEYAF YAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEI YEEV
Sbjct: 183 ITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEITYEEV 242

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNP+LFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE G+ALAEAVH
Sbjct: 243 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEDGVALAEAVH 302

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNLANPTALLLS V MLRHL+ +++A++I  AI+ TIAEGKYRTADLGGSS+
Sbjct: 303 GSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQAEQIHSAIINTIAEGKYRTADLGGSST 362

Query: 361 TTVFTNAICDHL 373
           TT FT AICDHL
Sbjct: 363 TTEFTKAICDHL 374

[Close]

BLAST of Moc01g22220 vs. TAIR 10
Match: AT3G09810.1 (isocitrate dehydrogenase VI )

HSP 1 Score: 586.3 bits (1510), Expect = 1.8e-167
Identity = 294/372 (79.03%), Postives = 330/372 (88.71%), Query Frame = 0


Query: 1   MASQLLRRALGSRSGQIFSLSSDLSSSAPVPLRAFSSAASPIRATLFPGDGIGPEIAESV 60
           M + L RR +G+ S QI   SS  S       RAF S+++PI+ATLFPGDGIGPEIAESV
Sbjct: 3   MTAFLARRLIGNGSSQILGTSSSSSGPFISVSRAFFSSSTPIKATLFPGDGIGPEIAESV 62

Query: 61  KQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSL 120
           KQVF  A+V I+W+E +V  ++DPRT SFLTW++L+SV +N+VGLKGPMATPIGKGHRSL
Sbjct: 63  KQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKNKVGLKGPMATPIGKGHRSL 122

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVV+GVVESLKI
Sbjct: 123 NLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVKGVVESLKI 182

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEV 240
           ITR+AS+RVAEYAF YAKTHGR++VSAIHKANIMQKTDGLFL+CC EVA KYPEI YE+V
Sbjct: 183 ITRKASMRVAEYAFLYAKTHGRKKVSAIHKANIMQKTDGLFLQCCDEVAAKYPEIYYEKV 242

Query: 241 VIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNP+LFDVLVMPNLYGDIISDLCAGL+GGLGLTPS NIGE GIALAEAVH
Sbjct: 243 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSMNIGEDGIALAEAVH 302

Query: 301 GSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSS 360
           GSAPDIAG NLANPTALLLS V MLRHL+L+ +A++I  AI+ TIAEGKYRTADLGGSS+
Sbjct: 303 GSAPDIAGMNLANPTALLLSGVMMLRHLKLNKQAEQIHSAIINTIAEGKYRTADLGGSST 362

Query: 361 TTVFTNAICDHL 373
           TT FT AICDHL
Sbjct: 363 TTDFTKAICDHL 374

[Close]

BLAST of Moc01g22220 vs. TAIR 10
Match: AT4G35260.1 (isocitrate dehydrogenase 1 )

HSP 1 Score: 329.7 bits (844), Expect = 3.0e-90
Identity = 168/332 (50.60%), Postives = 232/332 (69.88%), Query Frame = 0


Query: 40  SPIRATLFPGDGIGPEIAESVKQVFNTAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVR 99
           +P   TL PGDGIGP +  +V+QV      PI +E++ V  ++     S +  E +ES+R
Sbjct: 36  APRAVTLIPGDGIGPLVTNAVEQVMEAMHAPIFFEKYDVHGEM-----SRVPPEVMESIR 95

Query: 100 RNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENT 159
           +N+V LKG + TP+G G  SLN+ LRKEL+L+A++  C++LPG  TR++NV+++ IRENT
Sbjct: 96  KNKVCLKGGLKTPVGGGVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENT 155

Query: 160 EGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDG 219
           EGEY+GLEH+VV GVVESLK+IT+  S R+A+YAF YA  + R++V+A+HKANIM+  DG
Sbjct: 156 EGEYAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADG 215

Query: 220 LFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGG 279
           LFL+ CREVA+KYP I Y E+++DNCCM LV  P  FDV+V PNLYG+++++  AG+ GG
Sbjct: 216 LFLESCREVAKKYPSITYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLVANTAAGIAGG 275

Query: 280 LGLTPSCNIGEGGIAL---AEAVHGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADR 339
            G+ P  N+G         A A +     I  +N ANP ALLLSS  MLRHL+    ADR
Sbjct: 276 TGVMPGGNVGADHAVFEQGASAGNVGKDKIVLENKANPVALLLSSAMMLRHLQFPSFADR 335

Query: 340 IQDAILKTIAEGKYRTADLGGSSSTTVFTNAI 369
           ++ A+ K IAEGK RT DLGG+S+T    +A+
Sbjct: 336 LETAVKKVIAEGKCRTKDLGGTSTTQEVVDAV 362

[Close]

BLAST of Moc01g22220 vs. TAIR 10
Match: AT2G17130.1 (isocitrate dehydrogenase subunit 2 )

HSP 1 Score: 315.5 bits (807), Expect = 5.9e-86
Identity = 169/373 (45.31%), Postives = 242/373 (64.88%), Query Frame = 0


Query: 14  SGQIFSLSSDL---SSSAPVPLRAFS-----SAASPIRATLFPGDGIGPEIAESVKQVFN 73
           S Q FSL  +L   +S + +  R+ +         P   TL PGDG+GP +  +V+QV  
Sbjct: 2   SRQSFSLLKNLRSIASGSKIQTRSVTYMPRPGDGKPRPVTLIPGDGVGPLVTNAVQQVME 61

Query: 74  TAEVPIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSLNLTLR 133
               P+ +E   V   +    +       LES+++N+V LKG + TP+G G  SLN+ LR
Sbjct: 62  AMHAPVYFEPFEVHGDMKSLPEGL-----LESIKKNKVCLKGGLKTPVGGGVSSLNVNLR 121

Query: 134 KELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQA 193
           KEL+L+A++  C++LPG  +R++NV+++ IRENTEGEY+GLEH+VV GVVESLK+IT+  
Sbjct: 122 KELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKFC 181

Query: 194 SLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNC 253
           S R+A+YAF YA  + R++V+A+HKANIM+  DGLFL+ C+EVA+KYP I Y E+++DNC
Sbjct: 182 SERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDNC 241

Query: 254 CMMLVKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPD 313
           CM LV  P  FDV+V PNLYG+++++  AG+ GG G+ P  N+   G   A    G++  
Sbjct: 242 CMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNV---GAEYAVFEQGASAG 301

Query: 314 IAGKNL------ANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSS 373
             GK+       ANP ALLLSS  MLRHL+    ADR++ A+ + IAEG  RT DLGG+S
Sbjct: 302 NVGKDTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNS 361

[Close]

BLAST of Moc01g22220 vs. TAIR 10
Match: AT4G35650.1 (isocitrate dehydrogenase III )

HSP 1 Score: 312.8 bits (800), Expect = 3.8e-85
Identity = 169/362 (46.69%), Postives = 236/362 (65.19%), Query Frame = 0


Query: 19  SLSSDLSSSAPVPLRAFSSA---------ASPIRATLFPGDGIGPEIAESVKQVFNTAEV 78
           S+ + L ++ P P  + S +          +P   TL PGDGIGP +  +V+QV      
Sbjct: 7   SIFNRLLANPPSPFTSLSRSITYMPRPGDGAPRTVTLIPGDGIGPLVTGAVEQVMEAMHA 66

Query: 79  PIEWEEHYVSDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELN 138
           P+ +E + V   +    +     E +ESV+RN+V LKG +ATP+G G  SLN+ LRKEL+
Sbjct: 67  PVHFERYEVLGNMRKVPE-----EVIESVKRNKVCLKGGLATPVGGGVSSLNMQLRKELD 126

Query: 139 LYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRV 198
           ++A++  C ++PG  TR++NV+++ IRENTEGEYSGLEH+VV GVVESLK+IT+  S R+
Sbjct: 127 IFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERI 186

Query: 199 AEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMML 258
           A YAF YA  + R++V+A+HKANIM+  DGLFL+ CREVA+ Y  I Y E+++DNCCM L
Sbjct: 187 ARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKHYSGITYNEIIVDNCCMQL 246

Query: 259 VKNPSLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIG-EGGIALAEAVHGSAPD--I 318
           V  P  FDV+V PNLYG++I++  AG+ GG G+ P  N+G E  I    A  G+  +  +
Sbjct: 247 VAKPEQFDVMVTPNLYGNLIANTAAGIAGGTGVMPGGNVGAEHAIFEQGASAGNVGNDKM 306

Query: 319 AGKNLANPTALLLSSVTMLRHLELHDKADRIQDAILKTIAEGKYRTADLGGSSSTTVFTN 369
             +  ANP ALLLSS  MLRHL     ADR++ A+ + I EGKYRT DLGG  +T    +
Sbjct: 307 VEQKKANPVALLLSSAMMLRHLRFPTFADRLETAVKQVIKEGKYRTKDLGGDCTTQEVVD 363

The following BLAST results are available for this feature:

BLAST of Moc01g22220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143811.1	7.4e-208	100.00	isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial [Momordica cha...	[more]
XP_022925844.1	1.1e-200	95.97	isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial-like [Cucurbit...	[more]
XP_022977759.1	4.3e-200	95.70	isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial-like [Cucurbit...	[more]
XP_023518600.1	1.7e-199	95.43	isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial [Cucurbita pep...	[more]
XP_023544050.1	2.2e-199	95.43	isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial-like [Cucurbit...	[more]

BLAST of Moc01g22220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945K7	2.0e-179	84.95	Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial OS=Arabidopsis...	[more]
P29696	1.4e-169	86.89	3-isopropylmalate dehydrogenase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2...	[more]
Q8LG77	2.5e-166	79.03	Isocitrate dehydrogenase [NAD] catalytic subunit 6, mitochondrial OS=Arabidopsis...	[more]
Q9VWH4	2.5e-118	59.94	Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Drosophi...	[more]
Q55BI2	7.4e-118	61.24	Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial OS=Dictyostel...	[more]

BLAST of Moc01g22220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CRF5	3.6e-208	100.00	Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Momordica charantia OX=...	[more]
A0A6J1EDB6	5.5e-201	95.97	Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucurbita moschata OX=3...	[more]
A0A6J1ISA6	2.1e-200	95.70	Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucurbita maxima OX=366...	[more]
A0A6J1IAG8	1.8e-199	95.16	Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucurbita maxima OX=366...	[more]
A0A6J1HE33	1.8e-199	95.16	Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucurbita moschata OX=3...	[more]

BLAST of Moc01g22220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03290.1	1.4e-180	84.95	isocitrate dehydrogenase V	[more]
AT3G09810.1	1.8e-167	79.03	isocitrate dehydrogenase VI	[more]
AT4G35260.1	3.0e-90	50.60	isocitrate dehydrogenase 1	[more]
AT2G17130.1	5.9e-86	45.31	isocitrate dehydrogenase subunit 2	[more]
AT4G35650.1	3.8e-85	46.69	isocitrate dehydrogenase III	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024084	Isopropylmalate dehydrogenase-like domain	SMART	SM01329	Iso_dh_2	coord: 43..368 e-value: 1.3E-174 score: 596.0
IPR024084	Isopropylmalate dehydrogenase-like domain	PFAM	PF00180	Iso_dh	coord: 45..371 e-value: 7.5E-105 score: 350.9
None	No IPR available	GENE3D	3.40.718.10	Isopropylmalate Dehydrogenase	coord: 32..372 e-value: 1.8E-144 score: 483.0
None	No IPR available	PANTHER	PTHR11835:SF68	ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT, MITOCHONDRIAL	coord: 5..372
None	No IPR available	PANTHER	PTHR11835	DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE	coord: 5..372
None	No IPR available	SUPERFAMILY	53659	Isocitrate/Isopropylmalate dehydrogenase-like	coord: 42..372
IPR004434	Isocitrate dehydrogenase NAD-dependent	TIGRFAM	TIGR00175	TIGR00175	coord: 43..372 e-value: 3.7E-146 score: 484.5
IPR019818	Isocitrate/isopropylmalate dehydrogenase, conserved site	PROSITE	PS00470	IDH_IMDH	coord: 263..282

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g22220.1	Moc01g22220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006102	isocitrate metabolic process
biological_process	GO:0006099	tricarboxylic acid cycle
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004449	isocitrate dehydrogenase (NAD+) activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor