Moc01g20820 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g20820
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	protein NRT1/ PTR FAMILY 2.8-like
Location	chr1: 14543383 .. 14544584 (-)
RNA-Seq Expression	Moc01g20820
Synteny	Moc01g20820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAGATAGAGGGAAAAGAGGGGAAACATATATGGAGAATGACGCCATTGATCATTCTTCCTTGGATCCTCCACCCGTTGCTCCTTCAAAAGCAGCCGTCGGAGGAGGATGGAGAGCCGTGAGATTCATACTTGGTAAATTAATTAATACATAAATTTAATTAAATTTGTTTGTTTTTGTTTTTGTTCGGTTTGTGATTTGGTTTTTGGTTTATTATTGTATAATAGCAAATGAGACGTTTGAGAAATTGGCGTCGATGAGTTTGGTGGCGAATTTGATATTGTATTTGCACACGATGTACAATTTGGACAACGTGGCTTCGGCTAATGTGATCAACATCTGGGTGGCACCACCAATTTCGCTCCCATCGCCGGAGCTTTTCTTGCCGATGCTTACTTGGGAAGGTTTTACACTCTCCTCTTCGGCTCCATCGCATCCTTCTTCGTACGTTCCCATCTTCACAAAATTTTAACAAGTCTTACCGTTTCTAGTAAATCATCAAACTTTCTCCAGGTTCTTAACCCTATCCATTTAATATCAAATTATAAGTTTATAATAGTTAAATTTTTATACTTTTATAAGCTTTTAATTATTATTAAAGATTACGTGTTGGGTTAATTTGGAGTAGAAGTAGAAGTACAAAGTGGAGAGAGTTGGAATGAGTAAATGGGAAATGTGAAAAAAGCAAAGGGGTTTTGTGTACTCCAAAGAAAAAAAAAAGAAAACCGTGTCTTTGACATTCGCATCGTGTTGTTGTAATGTTATGGTCGGACAAAATTTATGGAATTAGAGAATACCATATTTTGTCGACCATTTCATTCATTGCTTGTGACATTATCATCGCGTATTAAAAATGACGTGGGATCAATTTGTTTTTGTTATTATATAAATAAAAAGGAGTATAATGTTCCGATAATAATGAACTTTTTCCACGTGGTTTTGAATATTCCAATATGTTAATAATAGGGCATGGGAGTGTTGACCCTAGGCGCTGGTCTTCCTGAGCTAAGGCCTCCCAGCTGCAGCCACAGCCAAGCTAAGTGCCCACAGCCGCAGCCTTGGCAACTCGCCTTCCTCTACCTCGGCCTTGGATTACTAGTGATCGGAGCCGGCGGAATAAGACCCTGCAACATCTCTTCGGTGCCGACCAGTTCGACACCACGACCTACAAGGGCCGAGCCAAGCTCGAAAGCTTCTTAA

mRNA sequence

ATGAGAGATAGAGGGAAAAGAGGGGAAACATATATGGAGAATGACGCCATTGATCATTCTTCCTTGGATCCTCCACCCGTTGCTCCTTCAAAAGCAGCCGTCGGAGGAGGATGGAGAGCCGTGAGATTCATACTTGCAAATGAGACGTTTGAGAAATTGGCGTCGATGAGTTTGGTGGCGAATTTGATATTGTATTTGCACACGATGTACAATTTGGACAACGTGGCTTCGGCTAATGTGATCAACATCTGGGTGGCACCACCAATTTCGCTCCCATCGCCGGAGCTTTTCTTGCCGATGCTTACTTGGGAAGGCGCTGGTCTTCCTGAGCTAAGGCCTCCCAGCTGCAGCCACAGCCAAGCTAAGTGCCCACAGCCGCAGCCTTGGCAACTCGCCTTCCTCTACCTCGGCCTTGGATTACTAGTGATCGGAGCCGGCGGAATAAGACCCTGCAACATCTCTTCGGTGCCGACCAGTTCGACACCACGACCTACAAGGGCCGAGCCAAGCTCGAAAGCTTCTTAA

Coding sequence (CDS)

ATGAGAGATAGAGGGAAAAGAGGGGAAACATATATGGAGAATGACGCCATTGATCATTCTTCCTTGGATCCTCCACCCGTTGCTCCTTCAAAAGCAGCCGTCGGAGGAGGATGGAGAGCCGTGAGATTCATACTTGCAAATGAGACGTTTGAGAAATTGGCGTCGATGAGTTTGGTGGCGAATTTGATATTGTATTTGCACACGATGTACAATTTGGACAACGTGGCTTCGGCTAATGTGATCAACATCTGGGTGGCACCACCAATTTCGCTCCCATCGCCGGAGCTTTTCTTGCCGATGCTTACTTGGGAAGGCGCTGGTCTTCCTGAGCTAAGGCCTCCCAGCTGCAGCCACAGCCAAGCTAAGTGCCCACAGCCGCAGCCTTGGCAACTCGCCTTCCTCTACCTCGGCCTTGGATTACTAGTGATCGGAGCCGGCGGAATAAGACCCTGCAACATCTCTTCGGTGCCGACCAGTTCGACACCACGACCTACAAGGGCCGAGCCAAGCTCGAAAGCTTCTTAA

Protein sequence

MRDRGKRGETYMENDAIDHSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWVAPPISLPSPELFLPMLTWEGAGLPELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNISSVPTSSTPRPTRAEPSSKAS

Homology

[Close]

BLAST of Moc01g20820 vs. NCBI nr
Match: XP_022151510.1 (protein NRT1/ PTR FAMILY 2.8-like [Momordica charantia])

HSP 1 Score: 232.6 bits (592), Expect = 2.5e-57
Identity = 127/165 (76.97%), Postives = 128/165 (77.58%), Query Frame = 0


Query: 12  MENDAIDHSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYN 71
           MENDAIDHSSLDPPPVAPSKAAV GGWRAVRFILANETFEKLASMSLVANLILYLHTMYN
Sbjct: 1   MENDAIDHSSLDPPPVAPSKAAV-GGWRAVRFILANETFEKLASMSLVANLILYLHTMYN 60

Query: 72  LDNVASANVINIWVAPPISLPSPELFLP----------------------MLTWEGAGLP 131
           LDNVASANVINIW       P    FL                       +LT  GAGLP
Sbjct: 61  LDNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFFGMGVLTL-GAGLP 120

Query: 132 ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
           ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS
Sbjct: 121 ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 163

[Close]

BLAST of Moc01g20820 vs. NCBI nr
Match: XP_038881205.1 (protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida] >XP_038881206.1 protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 167.9 bits (424), Expect = 7.6e-38
Identity = 90/157 (57.32%), Postives = 99/157 (63.06%), Query Frame = 0


Query: 19  HSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASA 78
           HSS D    AP K    GGWRAVRFIL NETFEKLASMSL+ NL+LYLHTMYNLDNVASA
Sbjct: 7   HSSSD----APRKKTARGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASA 66

Query: 79  NVINIWVAPPISLPSPELFLP---------------------MLTWEGAGLPELRPPSCS 138
            V  IW       P    FL                       +   GAGLP++RPP C 
Sbjct: 67  YVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLSLGAGLPQMRPPPCI 126

Query: 139 HSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
             ++ CPQPQPWQL FLYLGLGL+V+GAGGIRPCNIS
Sbjct: 127 SGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNIS 159

[Close]

BLAST of Moc01g20820 vs. NCBI nr
Match: KAA0045149.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucumis melo var. makuwa] >TYK23588.1 protein NRT1/ PTR FAMILY 2.8-like [Cucumis melo var. makuwa])

HSP 1 Score: 167.5 bits (423), Expect = 9.9e-38
Identity = 95/165 (57.58%), Postives = 105/165 (63.64%), Query Frame = 0


Query: 12  MENDAIDHSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYN 71
           MEN  + HSSLD    A  K    GGWRAVRFIL NETFEKLASMSL+ NL+LYLHTMYN
Sbjct: 1   MENGNL-HSSLD----ASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYN 60

Query: 72  LDNVASANVINIWVAPPISLPSPELFLP----------------------MLTWEGAGLP 131
           LDNVASA V  IW       P    FL                       +LT  GAGLP
Sbjct: 61  LDNVASAYVFQIWGGTTNFAPLAGAFLADTYLGRFYTLLFGSIASLLGMGVLTL-GAGLP 120

Query: 132 ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
           ++RPP C   ++ CPQP PWQL+FLYLGLGL+VIGAGGIRPCNIS
Sbjct: 121 QMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNIS 159

[Close]

BLAST of Moc01g20820 vs. NCBI nr
Match: XP_004142186.1 (protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KAE8649550.1 hypothetical protein Csa_017951 [Cucumis sativus])

HSP 1 Score: 166.8 bits (421), Expect = 1.7e-37
Identity = 93/165 (56.36%), Postives = 103/165 (62.42%), Query Frame = 0


Query: 12  MENDAIDHSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYN 71
           MEN  + H+SLD    A       GGWRAVRFIL NETFEKLASMSL+ NL+LYLHTMYN
Sbjct: 1   MENGNLLHASLD----ASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYN 60

Query: 72  LDNVASANVINIWVAPPISLPSPELFLP----------------------MLTWEGAGLP 131
           LDNVASA V  IW       P    FL                       +LT  GAGLP
Sbjct: 61  LDNVASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTL-GAGLP 120

Query: 132 ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
           ++RPP C   ++ CPQP PWQL FLYLGLGL+VIGAGGIRPCNIS
Sbjct: 121 QMRPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNIS 160

[Close]

BLAST of Moc01g20820 vs. NCBI nr
Match: KAG6603629.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 162.9 bits (411), Expect = 2.4e-36
Identity = 90/158 (56.96%), Postives = 100/158 (63.29%), Query Frame = 0


Query: 19  HSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASA 78
           HSS++ P +A       GGWRAVRFIL NETFEKLASMSL+ANL+LYLHTMYNLD+V SA
Sbjct: 7   HSSVNRPKIAL------GGWRAVRFILGNETFEKLASMSLIANLVLYLHTMYNLDSVDSA 66

Query: 79  NVINIWVAPPISLPSPELFLP----------------------MLTWEGAGLPELRPPSC 138
            V  IW            FL                       +LT  GAG PE+RPP C
Sbjct: 67  YVFQIWSGTTNFAALAGAFLADAYLGRFYTLLFGSFASLLGMGVLTL-GAGFPEMRPPPC 126

Query: 139 SHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
            H ++ CPQP PWQL FLYLGLGLLVIGAGGIRPCNIS
Sbjct: 127 RHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNIS 157

[Close]

BLAST of Moc01g20820 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 5.0e-24
Identity = 65/140 (46.43%), Postives = 79/140 (56.43%), Query Frame = 0


Query: 36  GGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWVAP-------- 95
           GGWRA+++I+ANE+FEKLASMSL+ NL +YL T YNL  V   NVINIW           
Sbjct: 19  GGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNILTLAG 78

Query: 96  ------------PISLPSPELFLPMLTWE-GAGLPELRPPSCSHSQAKCPQPQPWQLAFL 155
                        + L S   F+ M  +   A LP LRP +C        QP  WQL  L
Sbjct: 79  AFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQLGVL 138

[Close]

BLAST of Moc01g20820 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 6.9e-18
Identity = 53/140 (37.86%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 36  GGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWV-----APPIS 95
           GGWRA+ FIL NET EKL S+ + AN +LYL  +++++ V + NV  +W+     AP + 
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 96  LPSPELFLP----------------MLTWEGAGLPELRPPSCSHSQA-KCPQPQPWQLAF 154
               + ++                 M     A LP+L PP C++    +C  P   QL  
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

[Close]

BLAST of Moc01g20820 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.5e-17
Identity = 56/140 (40.00%), Postives = 74/140 (52.86%), Query Frame = 0


Query: 36  GGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWVA--------- 95
           GGWRAV FIL NET E+L S+ L+AN ++YL  +++L+ V +ANVINIW           
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 96  -----------PPISLPSPELFLPMLTWE-GAGLPELRPPSC-SHSQAKCPQPQPWQLAF 154
                        I+  S    L ++T    A  P+L P SC S     C  P   Q+  
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

[Close]

BLAST of Moc01g20820 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 6.5e-16
Identity = 51/150 (34.00%), Postives = 80/150 (53.33%), Query Frame = 0


Query: 27  VAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWVA 86
           V   K  V  GW+ + FI+ NETFEKL  +  ++NL++YL  ++NL ++ +A +IN +  
Sbjct: 36  VQNQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSG 95

Query: 87  --------------------PPISLPSPELFL-PMLTWEGAGLPELRPPSC-SHSQAKCP 146
                                 +S+     FL   +    A +P+L P +C + + + C 
Sbjct: 96  TINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICN 155

Query: 147 QPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
            P   Q+AFL +GLG LV+GAGGIRPCN++
Sbjct: 156 GPSGGQIAFLLMGLGFLVVGAGGIRPCNLA 185

[Close]

BLAST of Moc01g20820 vs. ExPASy Swiss-Prot
Match: Q944G5 (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 81.6 bits (200), Expect = 9.4e-15
Identity = 47/139 (33.81%), Postives = 74/139 (53.24%), Query Frame = 0


Query: 37  GWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWVA---------- 96
           GW+ + FI+ NETFEKL  +  ++NL++YL +++NL +  +A +IN +            
Sbjct: 64  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFGTFIAA 123

Query: 97  ----------PPISLPSPELFL-PMLTWEGAGLPELRPPSCSHSQAKCPQPQPWQLAFLY 155
                       +S+     FL   +    A +P L P +C  ++  C  P   Q+ FL 
Sbjct: 124 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACG-NKISCEGPSVGQILFLL 183

[Close]

BLAST of Moc01g20820 vs. ExPASy TrEMBL
Match: A0A6J1DBD9 (protein NRT1/ PTR FAMILY 2.8-like OS=Momordica charantia OX=3673 GN=LOC111019435 PE=3 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.2e-57
Identity = 127/165 (76.97%), Postives = 128/165 (77.58%), Query Frame = 0


Query: 12  MENDAIDHSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYN 71
           MENDAIDHSSLDPPPVAPSKAAV GGWRAVRFILANETFEKLASMSLVANLILYLHTMYN
Sbjct: 1   MENDAIDHSSLDPPPVAPSKAAV-GGWRAVRFILANETFEKLASMSLVANLILYLHTMYN 60

Query: 72  LDNVASANVINIWVAPPISLPSPELFLP----------------------MLTWEGAGLP 131
           LDNVASANVINIW       P    FL                       +LT  GAGLP
Sbjct: 61  LDNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFFGMGVLTL-GAGLP 120

Query: 132 ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
           ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS
Sbjct: 121 ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 163

[Close]

BLAST of Moc01g20820 vs. ExPASy TrEMBL
Match: A0A5D3DJD3 (Protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G00990 PE=3 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 4.8e-38
Identity = 95/165 (57.58%), Postives = 105/165 (63.64%), Query Frame = 0


Query: 12  MENDAIDHSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYN 71
           MEN  + HSSLD    A  K    GGWRAVRFIL NETFEKLASMSL+ NL+LYLHTMYN
Sbjct: 1   MENGNL-HSSLD----ASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYN 60

Query: 72  LDNVASANVINIWVAPPISLPSPELFLP----------------------MLTWEGAGLP 131
           LDNVASA V  IW       P    FL                       +LT  GAGLP
Sbjct: 61  LDNVASAYVFQIWGGTTNFAPLAGAFLADTYLGRFYTLLFGSIASLLGMGVLTL-GAGLP 120

Query: 132 ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
           ++RPP C   ++ CPQP PWQL+FLYLGLGL+VIGAGGIRPCNIS
Sbjct: 121 QMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNIS 159

[Close]

BLAST of Moc01g20820 vs. ExPASy TrEMBL
Match: A0A6J1F0P7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438173 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 2.2e-35
Identity = 95/165 (57.58%), Postives = 102/165 (61.82%), Query Frame = 0


Query: 12  MENDAIDHSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYN 71
           M+N  +  SSLDP P         GGWRAVRFILANETFEKLASMSL+ANLILYLHTMYN
Sbjct: 1   MDNGTL-RSSLDPSPKPQL-----GGWRAVRFILANETFEKLASMSLIANLILYLHTMYN 60

Query: 72  LDNVASANVINIWVAPPISLPSPELFLP----------------------MLTWEGAGLP 131
           LDNV SA V  IW       P    FL                       +LT  GA LP
Sbjct: 61  LDNVDSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTL-GAALP 120

Query: 132 ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
           E+RPP CS  +  C Q + WQLAFLYLGLGLLVIGAGGIRPCNIS
Sbjct: 121 EMRPPPCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNIS 158

[Close]

BLAST of Moc01g20820 vs. ExPASy TrEMBL
Match: A0A0A0KZQ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297490 PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 1.0e-32
Identity = 87/177 (49.15%), Postives = 103/177 (58.19%), Query Frame = 0


Query: 12  MENDAIDHSSLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYN 71
           MEN  + H+SLD    A       GGWRAVRFIL NETFEKLASMSL+ NL+LYLHTMYN
Sbjct: 1   MENGNLLHASLD----ASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYN 60

Query: 72  LDNVASANVINIWVAPPISLPSPELFLP----------------------MLTWEGAGLP 131
           LDNVASA V  IW       P    FL                       +LT  GAGLP
Sbjct: 61  LDNVASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTL-GAGLP 120

Query: 132 ELRPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNISSVPTSSTPRPTR 167
           ++RPP C   ++ CPQP PWQL FLYLGLGL+VIGAG +    +++      P+ T+
Sbjct: 121 QMRPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAGDMAKVVVATCRKWQIPKTTQ 172

[Close]

BLAST of Moc01g20820 vs. ExPASy TrEMBL
Match: A0A6J5VFE1 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS40192 PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 8.7e-32
Identity = 85/155 (54.84%), Postives = 96/155 (61.94%), Query Frame = 0


Query: 21  SLDPPPVAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANV 80
           S  PPP  P      GGWRAVRFIL NETFEK+ASMSLVANL++YLHT YNLDNV SANV
Sbjct: 15  SQSPPPKTPV-----GGWRAVRFILGNETFEKMASMSLVANLVMYLHTKYNLDNVVSANV 74

Query: 81  INIW-----VAP---------------PISLPSPELFLPMLTWE-GAGLPELRPPSCSHS 140
            NIW     +AP                +   S    L M T    AGL EL P +C   
Sbjct: 75  FNIWSGSCNIAPLFGAFVADTYIGKFYTLLFSSVASLLGMATLTLTAGLHELTPSACK-G 134

Query: 141 QAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
           Q +CPQP  WQLA LY GLGLLV+G+GG+RPCNI+
Sbjct: 135 QTECPQPNAWQLAVLYSGLGLLVVGSGGLRPCNIA 163

[Close]

BLAST of Moc01g20820 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 112.5 bits (280), Expect = 3.5e-25
Identity = 65/140 (46.43%), Postives = 79/140 (56.43%), Query Frame = 0


Query: 36  GGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWVAP-------- 95
           GGWRA+++I+ANE+FEKLASMSL+ NL +YL T YNL  V   NVINIW           
Sbjct: 19  GGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNILTLAG 78

Query: 96  ------------PISLPSPELFLPMLTWE-GAGLPELRPPSCSHSQAKCPQPQPWQLAFL 155
                        + L S   F+ M  +   A LP LRP +C        QP  WQL  L
Sbjct: 79  AFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQLGVL 138

[Close]

BLAST of Moc01g20820 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 92.0 bits (227), Expect = 4.9e-19
Identity = 53/140 (37.86%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 36  GGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWV-----APPIS 95
           GGWRA+ FIL NET EKL S+ + AN +LYL  +++++ V + NV  +W+     AP + 
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 96  LPSPELFLP----------------MLTWEGAGLPELRPPSCSHSQA-KCPQPQPWQLAF 154
               + ++                 M     A LP+L PP C++    +C  P   QL  
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

[Close]

BLAST of Moc01g20820 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-18
Identity = 56/140 (40.00%), Postives = 74/140 (52.86%), Query Frame = 0


Query: 36  GGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWVA--------- 95
           GGWRAV FIL NET E+L S+ L+AN ++YL  +++L+ V +ANVINIW           
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 96  -----------PPISLPSPELFLPMLTWE-GAGLPELRPPSC-SHSQAKCPQPQPWQLAF 154
                        I+  S    L ++T    A  P+L P SC S     C  P   Q+  
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

[Close]

BLAST of Moc01g20820 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 4.6e-17
Identity = 51/150 (34.00%), Postives = 80/150 (53.33%), Query Frame = 0


Query: 27  VAPSKAAVGGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWVA 86
           V   K  V  GW+ + FI+ NETFEKL  +  ++NL++YL  ++NL ++ +A +IN +  
Sbjct: 36  VQNQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSG 95

Query: 87  --------------------PPISLPSPELFL-PMLTWEGAGLPELRPPSC-SHSQAKCP 146
                                 +S+     FL   +    A +P+L P +C + + + C 
Sbjct: 96  TINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICN 155

Query: 147 QPQPWQLAFLYLGLGLLVIGAGGIRPCNIS 155
            P   Q+AFL +GLG LV+GAGGIRPCN++
Sbjct: 156 GPSGGQIAFLLMGLGFLVVGAGGIRPCNLA 185

[Close]

BLAST of Moc01g20820 vs. TAIR 10
Match: AT3G47960.1 (Major facilitator superfamily protein )

HSP 1 Score: 81.6 bits (200), Expect = 6.7e-16
Identity = 47/139 (33.81%), Postives = 74/139 (53.24%), Query Frame = 0


Query: 37  GWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWVA---------- 96
           GW+ + FI+ NETFEKL  +  ++NL++YL +++NL +  +A +IN +            
Sbjct: 64  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFGTFIAA 123

Query: 97  ----------PPISLPSPELFL-PMLTWEGAGLPELRPPSCSHSQAKCPQPQPWQLAFLY 155
                       +S+     FL   +    A +P L P +C  ++  C  P   Q+ FL 
Sbjct: 124 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACG-NKISCEGPSVGQILFLL 183

The following BLAST results are available for this feature:

BLAST of Moc01g20820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151510.1	2.5e-57	76.97	protein NRT1/ PTR FAMILY 2.8-like [Momordica charantia]	[more]
XP_038881205.1	7.6e-38	57.32	protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida] >XP_038881206.1...	[more]
KAA0045149.1	9.9e-38	57.58	protein NRT1/ PTR FAMILY 2.8-like [Cucumis melo var. makuwa] >TYK23588.1 protein...	[more]
XP_004142186.1	1.7e-37	56.36	protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KAE8649550.1 hypothetical protei...	[more]
KAG6603629.1	2.4e-36	56.96	Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Moc01g20820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E8X3	5.0e-24	46.43	Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2	[more]
Q9LFX9	6.9e-18	37.86	Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV...	[more]
Q8RX77	1.5e-17	40.00	Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV...	[more]
Q9LV10	6.5e-16	34.00	Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV...	[more]
Q944G5	9.4e-15	33.81	Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV...	[more]

BLAST of Moc01g20820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBD9	1.2e-57	76.97	protein NRT1/ PTR FAMILY 2.8-like OS=Momordica charantia OX=3673 GN=LOC111019435...	[more]
A0A5D3DJD3	4.8e-38	57.58	Protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1F0P7	2.2e-35	57.58	protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438173 ...	[more]
A0A0A0KZQ3	1.0e-32	49.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297490 PE=3 SV=1	[more]
A0A6J5VFE1	8.7e-32	54.84	Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS40192 PE=3 S...	[more]

BLAST of Moc01g20820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G28470.1	3.5e-25	46.43	Major facilitator superfamily protein	[more]
AT1G27080.1	4.9e-19	37.86	nitrate transporter 1.6	[more]
AT1G69870.1	1.1e-18	40.00	nitrate transporter 1.7	[more]
AT5G62680.1	4.6e-17	34.00	Major facilitator superfamily protein	[more]
AT3G47960.1	6.7e-16	33.81	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 96..163 e-value: 5.6E-9 score: 36.7 coord: 11..92 e-value: 8.0E-11 score: 42.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 154..174
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..33
None	No IPR available	PANTHER	PTHR11654:SF434	PEPTIDE/NITRATE TRANSPORTER	coord: 105..154
None	No IPR available	PANTHER	PTHR11654:SF434	PEPTIDE/NITRATE TRANSPORTER	coord: 28..87
IPR000109	Proton-dependent oligopeptide transporter family	PANTHER	PTHR11654	OLIGOPEPTIDE TRANSPORTER-RELATED	coord: 28..87 coord: 105..154

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g20820.1	Moc01g20820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity