Moc01g20600 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g20600
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionglutathione S-transferase U8-like
Locationchr1: 14404240 .. 14406515 (-)
RNA-Seq ExpressionMoc01g20600
SyntenyMoc01g20600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCCCCCAACATCCCCATGGAACAGCTCTTGCCAGGTTCTGGGCTAAGTACATAGATGACAAGGTAAGTTTTCTCTTTTCTGGGCTTCCTTTTTCTCATTCAGAGTGTACAAAAGTTAGAAAAAGTAGTTGATTTGCTGCATCGTTTTTCAAATTTCAGGTTGTGCCGGCAGTACTGAAAGCTGCTTTTAGTAAAGGAGAGGGAAAGGAGAAGGCTGTAGAACAAGTGTGGGAGACTTTGGAGCCACTGGAGAAGGAGATTGATGGGAAGAGGTTTTTTGGAGGAGACAGAATTGGGTTTGTGGACATTGTGGGCATTTTTGTAGGCTACTGGGTTCCTGCTATTGAAGAAAGTTTTGGGATTGAGCTGCTAAGAAGTGACAAATTTCCAAAACTAAGCAAATGGGTTCAAGAGCTTGTGAACCACAGCCTCGTGAAGGAGGTTCTGCCTCCAAAACTTCAACTAATTGCCTATATGCAAGCTGTTCACCCACCCAACTAGATATTGTTTTCTTTTCATATCTTAGAAATAAGATGAGCTTGAAAGAGGAGAGCAAATAATTCTCTCCCTCCTAATTTGTGTAGGTATAAAATTTTAAGTTTGTACTTTGGATTGAGAACTGAATTAAGAAAGAACGTTTTTCGATGTATAGTTAAAGATATTGTTTATACATAGAACTAGATAGAGTCAAACCTGATTAAAGTATGATAGGTGAAATAGATGAGTAACTTAGTAAGAAATGACCTACATAAGATAGACAAGTCTATAAGTTTGAACTTCATAGAGAGTTCGATAACTAATGTTGAAGTGAGGCTCTCTAGTTGAAAAACCGACCGAAGGTCATTTCCGGTGGTTCGTAGGTCGGACATGTCAGGTGTCATGGCTTGGAGTTAGTCAAAGATCGTGCCAACAGAATACCTTTTCATTGCACTCTGTGATTAAGAATGTAACGTTAGACCTATCCAAGGTGGACTTTGCTAATCCACTCGTTCCTTTATTCTTCACGTGGTAGTATAATTCTCTCCTAGTCGAGAGAAGTTTAATTATAAAAAAGTTTAGAGGATGGAGTAGTCAAACATGATGGCCAGATTAAATAACTTTTAATTAAAAAGGTTTGACCACTTACACAGAGGCTTACGACAACCATAACCTCCACTCCAAGAAATTGAACATTTCAAGCATTATTATACTAATCCTTTCTCCTCTCATAAGGAGCCAGCAGCCCAATACTCCCAAGTCCCAAGCTTTTTTTTTTTTTCTTCTTCTTTGCAGAGAAATTAATTTGAAAGAAATGGGTGAAGAAGTGCAGGTGTTTGGTTTCTGGGAAAGTGCGTACAGTCGCAGAGTGGAGTTGGCTCTCAAACTCAAAGCCGTGGAGTACCAATACATTGAGGAAGATTTGCCACACAACAAGAGCGATTTGCTTCTCAGATACAACCCAATTTACAAGAAAGTCCCTGTTCTCCTCCACCATGGAAAACCCATCGCAGAGTCTCTTGTCATCCTCCAATACATCGAAGATACTTGGAAGGCCAACCCCATGTTGCCTCAACACCCCCATCAACGAGCTCTCGCCAGGTTCTGGGCTAAGTACATCGATGACAAGGTAACGCTAGGAACGAGTCTTTTAAGATTCCGTTGGATATTGTTTCCATTTCTTGTTTTTAAATTTACTACGCAATCTAAACCTTTTCAAATTGTACGTAAGTTTTGGAAAGAATAAAAACAAGTTTTTTTTTCTTTTTTTTTTCTATTTCCAATTTGTTTTCTGATCATACTTGAATTCATAGATGTTAGGATCATGCAACAAAAACAACAAACATGGATATCATTCTATTAGGAATGCCAAGTGTAGGGACAATGGAAAGAAAGTTGAAAAGTCTGATATCCCATTTGATAATGATTCCATTTTTGTTTGTATCATTAATTTTTAGGTTGTGGGTGCGGTGGTGAAAGCTGCTGTAAGCAAAGGAGAGGAGAGAGAGAAGGCAGTAGAGGAAGCGAGGGAGGCGTTGGAAGAACTTGAGAAAGAGCTTAAAAGCAAGAAATTCTTTGGAGGACACAATATTGGGTTTGTGGACATTGTTGGCACCATCGTAGCCCACTGGGTTCCAGCCATTGAACAAAGTTTTGGGTTTGAGGTCCTCAAAGCTCACAACTTTCCAAATCTAACCAAATGGAGTGAAGAACTTGCAAACCACAGCCTGGTGAAGCAGGTTCTGCCCCCACAACATGAAATTGTTGCCTTCATGGAATCAGCTTGGTCAAATTAG

mRNA sequence

ATGTTCCCCCAACATCCCCATGGAACAGCTCTTGCCAGGTTCTGGGCTAAGTACATAGATGACAAGGTTGTGCCGGCAGTACTGAAAGCTGCTTTTAGTAAAGGAGAGGGAAAGGAGAAGGCTGTAGAACAAGTGTGGGAGACTTTGGAGCCACTGGAGAAGGAGATTGATGGGAAGAGGTTTTTTGGAGGAGACAGAATTGGGTTTGTGGACATTGTGGGCATTTTTGTAGGCTACTGGGTTCCTGCTATTGAAGAAAGTTTTGGGATTGAGCTGCTAAGAAGTGACAAATTTCCAAAACTAAGCAAATGGGTTCAAGAGCTTGTGAACCACAGCCTCGTGAAGGAGGTGTTTGGTTTCTGGGAAAGTGCGTACAGTCGCAGAGTGGAGTTGGCTCTCAAACTCAAAGCCGTGGAGTACCAATACATTGAGGAAGATTTGCCACACAACAAGAGCGATTTGCTTCTCAGATACAACCCAATTTACAAGAAAGTCCCTGTTCTCCTCCACCATGGAAAACCCATCGCAGAGTCTCTTGTCATCCTCCAATACATCGAAGATACTTGGAAGGCCAACCCCATGTTGCCTCAACACCCCCATCAACGAGCTCTCGCCAGGTTCTGGGCTAAGTACATCGATGACAAGGTTGTGGGTGCGGTGGTGAAAGCTGCTGTAAGCAAAGGAGAGGAGAGAGAGAAGGCAGTAGAGGAAGCGAGGGAGGCGTTGGAAGAACTTGAGAAAGAGCTTAAAAGCAAGAAATTCTTTGGAGGACACAATATTGGGTTTGTGGACATTGTTGGCACCATCGTAGCCCACTGGGTTCCAGCCATTGAACAAAGTTTTGGGTTTGAGGTCCTCAAAGCTCACAACTTTCCAAATCTAACCAAATGGAGTGAAGAACTTGCAAACCACAGCCTGGTGAAGCAGGTTCTGCCCCCACAACATGAAATTGTTGCCTTCATGGAATCAGCTTGGTCAAATTAG

Coding sequence (CDS)

ATGTTCCCCCAACATCCCCATGGAACAGCTCTTGCCAGGTTCTGGGCTAAGTACATAGATGACAAGGTTGTGCCGGCAGTACTGAAAGCTGCTTTTAGTAAAGGAGAGGGAAAGGAGAAGGCTGTAGAACAAGTGTGGGAGACTTTGGAGCCACTGGAGAAGGAGATTGATGGGAAGAGGTTTTTTGGAGGAGACAGAATTGGGTTTGTGGACATTGTGGGCATTTTTGTAGGCTACTGGGTTCCTGCTATTGAAGAAAGTTTTGGGATTGAGCTGCTAAGAAGTGACAAATTTCCAAAACTAAGCAAATGGGTTCAAGAGCTTGTGAACCACAGCCTCGTGAAGGAGGTGTTTGGTTTCTGGGAAAGTGCGTACAGTCGCAGAGTGGAGTTGGCTCTCAAACTCAAAGCCGTGGAGTACCAATACATTGAGGAAGATTTGCCACACAACAAGAGCGATTTGCTTCTCAGATACAACCCAATTTACAAGAAAGTCCCTGTTCTCCTCCACCATGGAAAACCCATCGCAGAGTCTCTTGTCATCCTCCAATACATCGAAGATACTTGGAAGGCCAACCCCATGTTGCCTCAACACCCCCATCAACGAGCTCTCGCCAGGTTCTGGGCTAAGTACATCGATGACAAGGTTGTGGGTGCGGTGGTGAAAGCTGCTGTAAGCAAAGGAGAGGAGAGAGAGAAGGCAGTAGAGGAAGCGAGGGAGGCGTTGGAAGAACTTGAGAAAGAGCTTAAAAGCAAGAAATTCTTTGGAGGACACAATATTGGGTTTGTGGACATTGTTGGCACCATCGTAGCCCACTGGGTTCCAGCCATTGAACAAAGTTTTGGGTTTGAGGTCCTCAAAGCTCACAACTTTCCAAATCTAACCAAATGGAGTGAAGAACTTGCAAACCACAGCCTGGTGAAGCAGGTTCTGCCCCCACAACATGAAATTGTTGCCTTCATGGAATCAGCTTGGTCAAATTAG

Protein sequence

MFPQHPHGTALARFWAKYIDDKVVPAVLKAAFSKGEGKEKAVEQVWETLEPLEKEIDGKRFFGGDRIGFVDIVGIFVGYWVPAIEESFGIELLRSDKFPKLSKWVQELVNHSLVKEVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPIAESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAVEEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLTKWSEELANHSLVKQVLPPQHEIVAFMESAWSN
Homology
BLAST of Moc01g20600 vs. NCBI nr
Match: XP_022151543.1 (probable glutathione S-transferase [Momordica charantia])

HSP 1 Score: 666.4 bits (1718), Expect = 1.3e-187
Identity = 326/327 (99.69%), Postives = 326/327 (99.69%), Query Frame = 0

Query: 1   MFPQHPHGTALARFWAKYIDDKVVPAVLKAAFSKGEGKEKAVEQVWETLEPLEKEIDGKR 60
           MFPQHPHGTALARFWAKYIDDKVVPAVLKAAFSKGEGKEKAVEQVWETLEPLEKEIDGKR
Sbjct: 83  MFPQHPHGTALARFWAKYIDDKVVPAVLKAAFSKGEGKEKAVEQVWETLEPLEKEIDGKR 142

Query: 61  FFGGDRIGFVDIVGIFVGYWVPAIEESFGIELLRSDKFPKLSKWVQELVNHSLVKEVFGF 120
           FFGGDRIGFVDIVGIFVGYWVPAIEESFGIEL RSDKFPKLSKWVQELVNHSLVKEVFGF
Sbjct: 143 FFGGDRIGFVDIVGIFVGYWVPAIEESFGIELXRSDKFPKLSKWVQELVNHSLVKEVFGF 202

Query: 121 WESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPIAESLV 180
           WESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPIAESLV
Sbjct: 203 WESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPIAESLV 262

Query: 181 ILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAVEEARE 240
           ILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAVEEARE
Sbjct: 263 ILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAVEEARE 322

Query: 241 ALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLTKWSEE 300
           ALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLTKWSEE
Sbjct: 323 ALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLTKWSEE 382

Query: 301 LANHSLVKQVLPPQHEIVAFMESAWSN 328
           LANHSLVKQVLPPQHEIVAFMESAWSN
Sbjct: 383 LANHSLVKQVLPPQHEIVAFMESAWSN 409

BLAST of Moc01g20600 vs. NCBI nr
Match: KAA3455051.1 (GST_C domain-containing protein/GST_N_3 domain-containing protein [Gossypium australe])

HSP 1 Score: 339.7 bits (870), Expect = 2.7e-89
Identity = 169/337 (50.15%), Postives = 230/337 (68.25%), Query Frame = 0

Query: 3   PQHPHGTALARFWAKYIDDKVVPAVLKAAFSKGEGKEKAVEQVWETLEPLEKEIDGKRFF 62
           PQ P+  A ARFW K+ID+K  P +  AA+S    +EK  ++  E L+ LE  ++GK+FF
Sbjct: 86  PQDPYDKATARFWVKFIDEKCFPTLWLAAWSPENEQEKVTKEACEYLKTLESALNGKKFF 145

Query: 63  GGDRIGFVDIVGIFVGYWVPAIEESFGIELLRSDKFPKLSKWVQELVNHSLVKE------ 122
           GG+ IG VDIV   VGY++   +E  G+ LL +DKFP+L +W ++  N S+VKE      
Sbjct: 146 GGETIGLVDIVASSVGYFIRVTQEIMGLNLLSADKFPQLFQWSEDFANCSIVKESLPPRD 205

Query: 123 ----------VFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVP 182
                     VFG W S +SR+VELAL+LK V Y YIEEDL +NKS LLL+YNP++KKVP
Sbjct: 206 KLLPFVKEVKVFGAWASPFSRKVELALRLKGVPYDYIEEDL-NNKSSLLLQYNPVHKKVP 265

Query: 183 VLLHHGKPIAESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVS 242
           VLLH+GK IAES+VIL+YIE+TWKA P+LPQ P+ +A+ARFW  +ID K   A+ K A S
Sbjct: 266 VLLHNGKSIAESIVILEYIEETWKAYPILPQDPNDKAMARFWINFIDGKCSSAIRKVAFS 325

Query: 243 KGEEREKAVEEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVL 302
             EEREKAVEEA E L+ LE  L  KKFFGG  IG VDIV   +A W+   ++  G E+L
Sbjct: 326 PEEEREKAVEEACECLKTLESALNGKKFFGGDTIGMVDIVALFIAFWIRPFQEIMGLELL 385

Query: 303 KAHNFPNLTKWSEELANHSLVKQVLPPQHEIVAFMES 324
            +  FPNL KW+++  + ++VK++LPP+ ++VA +++
Sbjct: 386 SSEKFPNLFKWTDDFVSCNIVKELLPPRDKLVAHIKA 421

BLAST of Moc01g20600 vs. NCBI nr
Match: XP_016900620.1 (PREDICTED: probable glutathione S-transferase [Cucumis melo] >KAA0045108.1 putative glutathione S-transferase [Cucumis melo var. makuwa] >TYK23630.1 putative glutathione S-transferase [Cucumis melo var. makuwa])

HSP 1 Score: 339.0 bits (868), Expect = 4.7e-89
Identity = 163/212 (76.89%), Postives = 189/212 (89.15%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           +VFGFWES +SRRVELALKLK VEYQY EEDLPHNKSDLLL+YNPI+KKVPVLLHHGKPI
Sbjct: 6   QVFGFWESPFSRRVELALKLKGVEYQYFEEDLPHNKSDLLLKYNPIHKKVPVLLHHGKPI 65

Query: 176 AESLVILQYIEDTWKAN-PMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKA 235
           AESLVIL+YI+D WK N P+LP HPHQRALARFWAK+IDDKVV AVVKAA S+GE+REKA
Sbjct: 66  AESLVILEYIDDVWKENYPILPHHPHQRALARFWAKFIDDKVVVAVVKAAGSRGEDREKA 125

Query: 236 VEEAREALEELEKEL-KSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPN 295
           +EEAREALE LEKE+ KS K FGG +IGFVDIVGT++A W+PAIE+ FGF++L   NFPN
Sbjct: 126 IEEAREALETLEKEIEKSNKLFGGDDIGFVDIVGTVIAGWIPAIEECFGFQLLTTDNFPN 185

Query: 296 LTKWSEELANHSLVKQVLPPQHEIVAFMESAW 326
           L KWS++LANHS+V Q+LPP+ EIVAFM++ W
Sbjct: 186 LIKWSDQLANHSIVNQILPPKTEIVAFMKANW 217

BLAST of Moc01g20600 vs. NCBI nr
Match: XP_011653662.1 (glutathione S-transferase U8 [Cucumis sativus] >KGN54321.1 hypothetical protein Csa_018162 [Cucumis sativus])

HSP 1 Score: 335.9 bits (860), Expect = 3.9e-88
Identity = 161/212 (75.94%), Postives = 189/212 (89.15%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           +V GFWESA+SRRVELALKLK VEYQY EEDLPHNKSDLLL+YNPI+KKVPVLLHHG+PI
Sbjct: 6   QVLGFWESAFSRRVELALKLKGVEYQYFEEDLPHNKSDLLLKYNPIHKKVPVLLHHGRPI 65

Query: 176 AESLVILQYIEDTWKAN-PMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKA 235
           AESLVIL+YI+D WK N P+LPQHPHQRALARFWAKYIDDKVVGAVVKAA S+GE+REKA
Sbjct: 66  AESLVILEYIDDIWKENYPILPQHPHQRALARFWAKYIDDKVVGAVVKAAGSRGEDREKA 125

Query: 236 VEEAREALEELEKEL-KSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPN 295
           +EEAREALE LEKE+ K+ + FGG +IGFVDIVGT++A WVPAIE+ F F++L   NFPN
Sbjct: 126 IEEAREALEALEKEIEKNNELFGGDDIGFVDIVGTVIAGWVPAIEECFEFQLLTTDNFPN 185

Query: 296 LTKWSEELANHSLVKQVLPPQHEIVAFMESAW 326
           L KWS++L  HS+V Q+LPP++EIVAFM++ W
Sbjct: 186 LIKWSDQLVQHSIVNQILPPKNEIVAFMKANW 217

BLAST of Moc01g20600 vs. NCBI nr
Match: XP_022978282.1 (glutathione S-transferase U8-like [Cucurbita maxima])

HSP 1 Score: 334.7 bits (857), Expect = 8.8e-88
Identity = 156/210 (74.29%), Postives = 184/210 (87.62%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           +VFGFWESA+SRRVELALKLK +EYQY EEDLPHNKS LLL+YNPI+KKVPVL+H+GKPI
Sbjct: 6   QVFGFWESAFSRRVELALKLKGIEYQYFEEDLPHNKSHLLLQYNPIHKKVPVLVHNGKPI 65

Query: 176 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAV 235
           +ESLVIL+YI++ WK NP+LPQHPH RA+ARFWAK+IDDKVV AVVKAA SKGEEREKAV
Sbjct: 66  SESLVILEYIDENWKTNPLLPQHPHDRAVARFWAKFIDDKVVVAVVKAAGSKGEEREKAV 125

Query: 236 EEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLT 295
           EEA EAL  LEKEL S+  FGG  +GFVD+VG+++  WVPAIE+ FGFEVL+ + FPNL 
Sbjct: 126 EEAGEALGALEKELGSRTLFGGERVGFVDVVGSVIVGWVPAIEECFGFEVLRVNKFPNLM 185

Query: 296 KWSEELANHSLVKQVLPPQHEIVAFMESAW 326
           KWSEE A H +VK++LPP+HEIVAFME+ W
Sbjct: 186 KWSEEFAKHGVVKEILPPKHEIVAFMEANW 215

BLAST of Moc01g20600 vs. ExPASy Swiss-Prot
Match: Q9SR36 (Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 6.2e-60
Identity = 108/213 (50.70%), Postives = 157/213 (73.71%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ G W S +S+RVE+ LKLK + Y+YIEED+  N+S +LL+YNPI+KKVPVL+H+G+ I
Sbjct: 8   KLLGLWGSPFSKRVEMVLKLKGIPYEYIEEDVYGNRSPMLLKYNPIHKKVPVLIHNGRSI 67

Query: 176 AESLVILQYIEDTWK-ANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKA 235
           AESLVI++YIEDTWK  + +LPQ P++RA+ARFWAKY+D+KV+ AV KA      EREK 
Sbjct: 68  AESLVIVEYIEDTWKTTHTILPQDPYERAMARFWAKYVDEKVMLAVKKACWGPESEREKE 127

Query: 236 VEEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNL 295
           V+EA E L+ LEKEL  K FFGG  IGFVDI    + +W+   +++ G  ++ A  FP L
Sbjct: 128 VKEAYEGLKCLEKELGDKLFFGGETIGFVDIAADFIGYWLGIFQEASGVTIMTAEEFPKL 187

Query: 296 TKWSEELANHSLVKQVLPPQHEIVAFMESAWSN 328
            +WSE+   ++ +K+VLPP+ ++VA +++ + +
Sbjct: 188 QRWSEDFVGNNFIKEVLPPKEKLVAVLKAMFGS 220

BLAST of Moc01g20600 vs. ExPASy Swiss-Prot
Match: P32110 (Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.1e-60
Identity = 112/213 (52.58%), Postives = 155/213 (72.77%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ G   S +  RV++ALKLK VEY+++EE+L  NKSDLLL+YNP++KKVPV +H+ +PI
Sbjct: 9   KLLGIVGSPFVCRVQIALKLKGVEYKFLEENL-GNKSDLLLKYNPVHKKVPVFVHNEQPI 68

Query: 176 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGE-EREKA 235
           AESLVI++YI++TWK NP+LP  P+QRALARFW+K+IDDK+VGAV K+  +  E EREK 
Sbjct: 69  AESLVIVEYIDETWKNNPILPSDPYQRALARFWSKFIDDKIVGAVSKSVFTVDEKEREKN 128

Query: 236 VEEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNL 295
           VEE  EAL+ LE ELK KKFFGG   G VDI    +A W+P  ++  G ++  +  FP L
Sbjct: 129 VEETYEALQFLENELKDKKFFGGEEFGLVDIAAVFIAFWIPIFQEIAGLQLFTSEKFPIL 188

Query: 296 TKWSEELANHSLVKQVLPPQHEIVAFMESAWSN 328
            KWS+E  NH  V +VLPP+  + A+ ++ + +
Sbjct: 189 YKWSQEFLNHPFVHEVLPPRDPLFAYFKARYES 220

BLAST of Moc01g20600 vs. ExPASy Swiss-Prot
Match: Q03663 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 6.4e-57
Identity = 108/208 (51.92%), Postives = 147/208 (70.67%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ GFW S +S RVE ALK+K V+Y+YIEED   NKS LLL+ NP+YKKVPVL+H+GKPI
Sbjct: 5   KLLGFWYSPFSHRVEWALKIKGVKYEYIEED-RDNKSSLLLQSNPVYKKVPVLIHNGKPI 64

Query: 176 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAV 235
            ES++IL+YI++T++   +LP+ P+ RALARFWAK++DDK V AVV     KGEE+EK  
Sbjct: 65  VESMIILEYIDETFEGPSILPKDPYDRALARFWAKFLDDK-VAAVVNTFFRKGEEQEKGK 124

Query: 236 EEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLT 295
           EE  E L+ L+ ELK KKFF G   GF DI   +V  W+   E+ +G  ++K+  FPN +
Sbjct: 125 EEVYEMLKVLDNELKDKKFFAGDKFGFADIAANLVGFWLGVFEEGYGDVLVKSEKFPNFS 184

Query: 296 KWSEELANHSLVKQVLPPQHEIVAFMES 324
           KW +E  N S V + LPP+ E++AF  +
Sbjct: 185 KWRDEYINCSQVNESLPPRDELLAFFRA 210

BLAST of Moc01g20600 vs. ExPASy Swiss-Prot
Match: Q03662 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.6e-55
Identity = 111/208 (53.37%), Postives = 148/208 (71.15%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ GFW S +SRRVE ALK+K V+Y+YIEED   NKS LLL+ NPI+KKVPVL+H+GK I
Sbjct: 5   KLLGFWYSPFSRRVEWALKIKGVKYEYIEED-RDNKSSLLLQSNPIHKKVPVLIHNGKRI 64

Query: 176 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAV 235
            ES+VIL+YI++T++   +LP+ P+ RALARFWAK++DDK V AVVK  + KGEE+EK  
Sbjct: 65  VESMVILEYIDETFEGPSILPKDPYDRALARFWAKFLDDK-VPAVVKTFLRKGEEQEKDK 124

Query: 236 EEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLT 295
           EE  E L+ L+ ELK KKFF G   GF DI   +VA W+   E++ G  ++ +  FPN  
Sbjct: 125 EEVCEMLKVLDNELKDKKFFVGDKFGFADIAANLVAFWLGVFEEASGVVLVTSEKFPNFC 184

Query: 296 KWSEELANHSLVKQVLPPQHEIVAFMES 324
           KW  E  N S +K+ LPP+ E++AF  S
Sbjct: 185 KWRGEYINCSQIKESLPPRDELLAFYRS 210

BLAST of Moc01g20600 vs. ExPASy Swiss-Prot
Match: Q03664 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-53
Identity = 104/208 (50.00%), Postives = 145/208 (69.71%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ GFW S ++ RVE ALKLK V+Y+YIEED   NKS LLL+ NP++KKVPVL+H+GKPI
Sbjct: 5   KLLGFWYSPFTHRVEWALKLKGVKYEYIEED-RDNKSSLLLQSNPVHKKVPVLIHNGKPI 64

Query: 176 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAV 235
            ES+VIL+YI++T++   +LP+ P+ RALARFW+K++ DK V AVV     KGEE+EK  
Sbjct: 65  VESMVILEYIDETFEGPSILPKDPYDRALARFWSKFLGDK-VAAVVNTFFRKGEEQEKGK 124

Query: 236 EEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLT 295
           EE  E L+ L+ ELK KKFF G   GF DI   +V  W+   E+ +G  ++ +  FPN +
Sbjct: 125 EEVYEMLKVLDNELKDKKFFVGDKFGFADIAANLVGFWLGVFEEGYGVVLVTSEKFPNFS 184

Query: 296 KWSEELANHSLVKQVLPPQHEIVAFMES 324
           +W +E  N S VK+ LP + E++AF  +
Sbjct: 185 RWRDEYINCSQVKESLPSRDELLAFFRA 210

BLAST of Moc01g20600 vs. ExPASy TrEMBL
Match: A0A6J1DCG3 (probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC111019458 PE=4 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 6.2e-188
Identity = 326/327 (99.69%), Postives = 326/327 (99.69%), Query Frame = 0

Query: 1   MFPQHPHGTALARFWAKYIDDKVVPAVLKAAFSKGEGKEKAVEQVWETLEPLEKEIDGKR 60
           MFPQHPHGTALARFWAKYIDDKVVPAVLKAAFSKGEGKEKAVEQVWETLEPLEKEIDGKR
Sbjct: 83  MFPQHPHGTALARFWAKYIDDKVVPAVLKAAFSKGEGKEKAVEQVWETLEPLEKEIDGKR 142

Query: 61  FFGGDRIGFVDIVGIFVGYWVPAIEESFGIELLRSDKFPKLSKWVQELVNHSLVKEVFGF 120
           FFGGDRIGFVDIVGIFVGYWVPAIEESFGIEL RSDKFPKLSKWVQELVNHSLVKEVFGF
Sbjct: 143 FFGGDRIGFVDIVGIFVGYWVPAIEESFGIELXRSDKFPKLSKWVQELVNHSLVKEVFGF 202

Query: 121 WESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPIAESLV 180
           WESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPIAESLV
Sbjct: 203 WESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPIAESLV 262

Query: 181 ILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAVEEARE 240
           ILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAVEEARE
Sbjct: 263 ILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAVEEARE 322

Query: 241 ALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLTKWSEE 300
           ALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLTKWSEE
Sbjct: 323 ALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLTKWSEE 382

Query: 301 LANHSLVKQVLPPQHEIVAFMESAWSN 328
           LANHSLVKQVLPPQHEIVAFMESAWSN
Sbjct: 383 LANHSLVKQVLPPQHEIVAFMESAWSN 409

BLAST of Moc01g20600 vs. ExPASy TrEMBL
Match: A0A5B6UAM0 (GST_C domain-containing protein/GST_N_3 domain-containing protein OS=Gossypium australe OX=47621 GN=EPI10_018120 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 1.3e-89
Identity = 169/337 (50.15%), Postives = 230/337 (68.25%), Query Frame = 0

Query: 3   PQHPHGTALARFWAKYIDDKVVPAVLKAAFSKGEGKEKAVEQVWETLEPLEKEIDGKRFF 62
           PQ P+  A ARFW K+ID+K  P +  AA+S    +EK  ++  E L+ LE  ++GK+FF
Sbjct: 86  PQDPYDKATARFWVKFIDEKCFPTLWLAAWSPENEQEKVTKEACEYLKTLESALNGKKFF 145

Query: 63  GGDRIGFVDIVGIFVGYWVPAIEESFGIELLRSDKFPKLSKWVQELVNHSLVKE------ 122
           GG+ IG VDIV   VGY++   +E  G+ LL +DKFP+L +W ++  N S+VKE      
Sbjct: 146 GGETIGLVDIVASSVGYFIRVTQEIMGLNLLSADKFPQLFQWSEDFANCSIVKESLPPRD 205

Query: 123 ----------VFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVP 182
                     VFG W S +SR+VELAL+LK V Y YIEEDL +NKS LLL+YNP++KKVP
Sbjct: 206 KLLPFVKEVKVFGAWASPFSRKVELALRLKGVPYDYIEEDL-NNKSSLLLQYNPVHKKVP 265

Query: 183 VLLHHGKPIAESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVS 242
           VLLH+GK IAES+VIL+YIE+TWKA P+LPQ P+ +A+ARFW  +ID K   A+ K A S
Sbjct: 266 VLLHNGKSIAESIVILEYIEETWKAYPILPQDPNDKAMARFWINFIDGKCSSAIRKVAFS 325

Query: 243 KGEEREKAVEEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVL 302
             EEREKAVEEA E L+ LE  L  KKFFGG  IG VDIV   +A W+   ++  G E+L
Sbjct: 326 PEEEREKAVEEACECLKTLESALNGKKFFGGDTIGMVDIVALFIAFWIRPFQEIMGLELL 385

Query: 303 KAHNFPNLTKWSEELANHSLVKQVLPPQHEIVAFMES 324
            +  FPNL KW+++  + ++VK++LPP+ ++VA +++
Sbjct: 386 SSEKFPNLFKWTDDFVSCNIVKELLPPRDKLVAHIKA 421

BLAST of Moc01g20600 vs. ExPASy TrEMBL
Match: A0A5A7TQE7 (Putative glutathione S-transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001470 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 2.3e-89
Identity = 163/212 (76.89%), Postives = 189/212 (89.15%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           +VFGFWES +SRRVELALKLK VEYQY EEDLPHNKSDLLL+YNPI+KKVPVLLHHGKPI
Sbjct: 6   QVFGFWESPFSRRVELALKLKGVEYQYFEEDLPHNKSDLLLKYNPIHKKVPVLLHHGKPI 65

Query: 176 AESLVILQYIEDTWKAN-PMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKA 235
           AESLVIL+YI+D WK N P+LP HPHQRALARFWAK+IDDKVV AVVKAA S+GE+REKA
Sbjct: 66  AESLVILEYIDDVWKENYPILPHHPHQRALARFWAKFIDDKVVVAVVKAAGSRGEDREKA 125

Query: 236 VEEAREALEELEKEL-KSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPN 295
           +EEAREALE LEKE+ KS K FGG +IGFVDIVGT++A W+PAIE+ FGF++L   NFPN
Sbjct: 126 IEEAREALETLEKEIEKSNKLFGGDDIGFVDIVGTVIAGWIPAIEECFGFQLLTTDNFPN 185

Query: 296 LTKWSEELANHSLVKQVLPPQHEIVAFMESAW 326
           L KWS++LANHS+V Q+LPP+ EIVAFM++ W
Sbjct: 186 LIKWSDQLANHSIVNQILPPKTEIVAFMKANW 217

BLAST of Moc01g20600 vs. ExPASy TrEMBL
Match: A0A1S4DY29 (probable glutathione S-transferase OS=Cucumis melo OX=3656 GN=LOC103490764 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 2.3e-89
Identity = 163/212 (76.89%), Postives = 189/212 (89.15%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           +VFGFWES +SRRVELALKLK VEYQY EEDLPHNKSDLLL+YNPI+KKVPVLLHHGKPI
Sbjct: 6   QVFGFWESPFSRRVELALKLKGVEYQYFEEDLPHNKSDLLLKYNPIHKKVPVLLHHGKPI 65

Query: 176 AESLVILQYIEDTWKAN-PMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKA 235
           AESLVIL+YI+D WK N P+LP HPHQRALARFWAK+IDDKVV AVVKAA S+GE+REKA
Sbjct: 66  AESLVILEYIDDVWKENYPILPHHPHQRALARFWAKFIDDKVVVAVVKAAGSRGEDREKA 125

Query: 236 VEEAREALEELEKEL-KSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPN 295
           +EEAREALE LEKE+ KS K FGG +IGFVDIVGT++A W+PAIE+ FGF++L   NFPN
Sbjct: 126 IEEAREALETLEKEIEKSNKLFGGDDIGFVDIVGTVIAGWIPAIEECFGFQLLTTDNFPN 185

Query: 296 LTKWSEELANHSLVKQVLPPQHEIVAFMESAW 326
           L KWS++LANHS+V Q+LPP+ EIVAFM++ W
Sbjct: 186 LIKWSDQLANHSIVNQILPPKTEIVAFMKANW 217

BLAST of Moc01g20600 vs. ExPASy TrEMBL
Match: A0A0A0L2G1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G303690 PE=3 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.9e-88
Identity = 161/212 (75.94%), Postives = 189/212 (89.15%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           +V GFWESA+SRRVELALKLK VEYQY EEDLPHNKSDLLL+YNPI+KKVPVLLHHG+PI
Sbjct: 6   QVLGFWESAFSRRVELALKLKGVEYQYFEEDLPHNKSDLLLKYNPIHKKVPVLLHHGRPI 65

Query: 176 AESLVILQYIEDTWKAN-PMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKA 235
           AESLVIL+YI+D WK N P+LPQHPHQRALARFWAKYIDDKVVGAVVKAA S+GE+REKA
Sbjct: 66  AESLVILEYIDDIWKENYPILPQHPHQRALARFWAKYIDDKVVGAVVKAAGSRGEDREKA 125

Query: 236 VEEAREALEELEKEL-KSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPN 295
           +EEAREALE LEKE+ K+ + FGG +IGFVDIVGT++A WVPAIE+ F F++L   NFPN
Sbjct: 126 IEEAREALEALEKEIEKNNELFGGDDIGFVDIVGTVIAGWVPAIEECFEFQLLTTDNFPN 185

Query: 296 LTKWSEELANHSLVKQVLPPQHEIVAFMESAW 326
           L KWS++L  HS+V Q+LPP++EIVAFM++ W
Sbjct: 186 LIKWSDQLVQHSIVNQILPPKNEIVAFMKANW 217

BLAST of Moc01g20600 vs. TAIR 10
Match: AT3G09270.1 (glutathione S-transferase TAU 8 )

HSP 1 Score: 232.6 bits (592), Expect = 4.4e-61
Identity = 108/213 (50.70%), Postives = 157/213 (73.71%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ G W S +S+RVE+ LKLK + Y+YIEED+  N+S +LL+YNPI+KKVPVL+H+G+ I
Sbjct: 8   KLLGLWGSPFSKRVEMVLKLKGIPYEYIEEDVYGNRSPMLLKYNPIHKKVPVLIHNGRSI 67

Query: 176 AESLVILQYIEDTWK-ANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKA 235
           AESLVI++YIEDTWK  + +LPQ P++RA+ARFWAKY+D+KV+ AV KA      EREK 
Sbjct: 68  AESLVIVEYIEDTWKTTHTILPQDPYERAMARFWAKYVDEKVMLAVKKACWGPESEREKE 127

Query: 236 VEEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNL 295
           V+EA E L+ LEKEL  K FFGG  IGFVDI    + +W+   +++ G  ++ A  FP L
Sbjct: 128 VKEAYEGLKCLEKELGDKLFFGGETIGFVDIAADFIGYWLGIFQEASGVTIMTAEEFPKL 187

Query: 296 TKWSEELANHSLVKQVLPPQHEIVAFMESAWSN 328
            +WSE+   ++ +K+VLPP+ ++VA +++ + +
Sbjct: 188 QRWSEDFVGNNFIKEVLPPKEKLVAVLKAMFGS 220

BLAST of Moc01g20600 vs. TAIR 10
Match: AT2G29420.1 (glutathione S-transferase tau 7 )

HSP 1 Score: 211.1 bits (536), Expect = 1.4e-54
Identity = 93/208 (44.71%), Postives = 148/208 (71.15%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ G W S +SRR+E+AL LK V Y+++E+D+  NKS LLL+ NP++K +PVL+H+GKPI
Sbjct: 11  KLLGMWASPFSRRIEIALTLKGVSYEFLEQDIT-NKSSLLLQLNPVHKMIPVLVHNGKPI 70

Query: 176 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAV 235
           +ESLVIL+YI++TW+ NP+LPQ P++R +ARFW+K++D+++    +K     G+ER+  V
Sbjct: 71  SESLVILEYIDETWRDNPILPQDPYERTMARFWSKFVDEQIYVTAMKVVGKTGKERDAVV 130

Query: 236 EEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLT 295
           E  R+ L  LEKEL  K F GG ++GFVDIV T+VA W+   E+  G +V+    FP + 
Sbjct: 131 EATRDLLMFLEKELVGKDFLGGKSLGFVDIVATLVAFWLMRTEEIVGVKVVPVEKFPEIH 190

Query: 296 KWSEELANHSLVKQVLPPQHEIVAFMES 324
           +W + L  + ++K+ +PP+ E + ++ +
Sbjct: 191 RWVKNLLGNDVIKKCIPPEDEHLKYIRA 217

BLAST of Moc01g20600 vs. TAIR 10
Match: AT2G29490.1 (glutathione S-transferase TAU 1 )

HSP 1 Score: 194.5 bits (493), Expect = 1.3e-49
Identity = 95/207 (45.89%), Postives = 142/207 (68.60%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ GFW S +SRRVE+ALKLK V Y+Y+EEDLP NK+ LLL  NP++KKVPVL+H+ K +
Sbjct: 9   KLLGFWASPFSRRVEMALKLKGVPYEYLEEDLP-NKTPLLLELNPLHKKVPVLVHNDKIL 68

Query: 176 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAV 235
            ES +IL+YI+ TWK +P+LPQ P+++A+ARFWAK+IDD+++    ++ V   + RE A+
Sbjct: 69  LESHLILEYIDQTWKNSPILPQDPYEKAMARFWAKFIDDQILTLGFRSLVKAEKGREVAI 128

Query: 236 EEAREALEELEKELKSKKFFGGHNIGFVD-IVGTIVAHWVPAIEQSFGFEVLKAHNFPNL 295
           EE RE L  LEKE+  K FFGG  IGF+D I G+++   +  + +  G +++    FP L
Sbjct: 129 EETRELLMFLEKEVTGKDFFGGKTIGFLDMIAGSMIPFCLARLWKGIGIDMIPEEKFPEL 188

Query: 296 TKWSEELANHSLVKQVLPPQHEIVAFM 322
            +W + L     V+  +PP+ + +  M
Sbjct: 189 NRWIKNLEEVEAVRGCIPPREKQIERM 214

BLAST of Moc01g20600 vs. TAIR 10
Match: AT2G29460.1 (glutathione S-transferase tau 4 )

HSP 1 Score: 186.4 bits (472), Expect = 3.6e-47
Identity = 93/209 (44.50%), Postives = 145/209 (69.38%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ GFW S ++RRVE+A KLK V Y+Y+E+D+  NKS LLL+ NP+YKKVPVL++ GK +
Sbjct: 9   KLLGFWASPFTRRVEMAFKLKGVPYEYLEQDIV-NKSPLLLQINPVYKKVPVLVYKGKIL 68

Query: 176 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEER-EKA 235
           +ES VIL+YI+  WK NP+LPQ P+++A+A FWAK++D++ VG V   +V+K E+  E A
Sbjct: 69  SESHVILEYIDQIWKNNPILPQDPYEKAMALFWAKFVDEQ-VGPVAFMSVAKAEKGVEVA 128

Query: 236 VEEAREALEELEKELKSKKFFGGHNIGFVDIV-GTIVAHWVPAIEQSFGFEVLKAHNFPN 295
           ++EA+E    LEKE+  K FFGG  IGF+D+V G+++   +    +  G +++    FP 
Sbjct: 129 IKEAQELFMFLEKEVTGKDFFGGKTIGFLDLVAGSMIPFCLARGWEGMGIDMIPEEKFPE 188

Query: 296 LTKWSEELANHSLVKQVLPPQHEIVAFME 323
           L +W + L    +V++ +PP+ E +  M+
Sbjct: 189 LNRWIKNLKEIEIVRECIPPREEQIEHMK 215

BLAST of Moc01g20600 vs. TAIR 10
Match: AT2G29480.1 (glutathione S-transferase tau 2 )

HSP 1 Score: 183.7 bits (465), Expect = 2.3e-46
Identity = 90/208 (43.27%), Postives = 138/208 (66.35%), Query Frame = 0

Query: 116 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 175
           ++ GFW S +SRRVE+ALKLK V Y+Y+EEDLP  KS LLL  NP++KKVPVL+H+ K +
Sbjct: 9   KLLGFWISPFSRRVEMALKLKGVPYEYLEEDLP-KKSTLLLELNPVHKKVPVLVHNDKLL 68

Query: 176 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAV 235
           +ES VIL+YI+ TW  NP+LP  P+++A+ RFWAK++D++++       V   +  + A+
Sbjct: 69  SESHVILEYIDQTWNNNPILPHDPYEKAMVRFWAKFVDEQILPVGFMPLVKAEKGIDVAI 128

Query: 236 EEAREALEELEKELKSKKFFGGHNIGFVDIV-GTIVAHWVPAIEQSFGFEVLKAHNFPNL 295
           EE RE L  LEKE+  K FFGG  IGF+D+V G+++   +    +  G ++     FP L
Sbjct: 129 EEIREMLMFLEKEVTGKDFFGGKTIGFLDMVAGSMIPFCLARAWECLGIDMTPEDTFPEL 188

Query: 296 TKWSEELANHSLVKQVLPPQHEIVAFME 323
            +W + L    +V++ +PP+ + +  M+
Sbjct: 189 NRWIKNLNEVEIVRECIPPKEKHIERMK 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151543.11.3e-18799.69probable glutathione S-transferase [Momordica charantia][more]
KAA3455051.12.7e-8950.15GST_C domain-containing protein/GST_N_3 domain-containing protein [Gossypium aus... [more]
XP_016900620.14.7e-8976.89PREDICTED: probable glutathione S-transferase [Cucumis melo] >KAA0045108.1 putat... [more]
XP_011653662.13.9e-8875.94glutathione S-transferase U8 [Cucumis sativus] >KGN54321.1 hypothetical protein ... [more]
XP_022978282.18.8e-8874.29glutathione S-transferase U8-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SR366.2e-6050.70Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1[more]
P321108.1e-6052.58Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1[more]
Q036636.4e-5751.92Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q036621.6e-5553.37Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q036641.1e-5350.00Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DCG36.2e-18899.69probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC11101945... [more]
A0A5B6UAM01.3e-8950.15GST_C domain-containing protein/GST_N_3 domain-containing protein OS=Gossypium a... [more]
A0A5A7TQE72.3e-8976.89Putative glutathione S-transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S4DY292.3e-8976.89probable glutathione S-transferase OS=Cucumis melo OX=3656 GN=LOC103490764 PE=3 ... [more]
A0A0A0L2G11.9e-8875.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G303690 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G09270.14.4e-6150.70glutathione S-transferase TAU 8 [more]
AT2G29420.11.4e-5444.71glutathione S-transferase tau 7 [more]
AT2G29490.11.3e-4945.89glutathione S-transferase TAU 1 [more]
AT2G29460.13.6e-4744.50glutathione S-transferase tau 4 [more]
AT2G29480.12.3e-4643.27glutathione S-transferase tau 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 225..255
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 115..194
e-value: 5.6E-35
score: 121.2
NoneNo IPR availableGENE3D1.20.1050.10coord: 195..326
e-value: 3.8E-45
score: 155.5
NoneNo IPR availableGENE3D1.20.1050.10coord: 2..114
e-value: 1.3E-28
score: 101.7
NoneNo IPR availablePANTHERPTHR11260GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAININGcoord: 117..323
NoneNo IPR availablePANTHERPTHR11260:SF556GLUTATHIONE S-TRANSFERASE-RELATEDcoord: 2..117
coord: 117..323
NoneNo IPR availablePANTHERPTHR11260GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAININGcoord: 2..117
NoneNo IPR availableCDDcd03058GST_N_Taucoord: 117..189
e-value: 6.50217E-37
score: 125.468
IPR004045Glutathione S-transferase, N-terminalPFAMPF02798GST_Ncoord: 117..186
e-value: 1.3E-15
score: 57.5
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 113..193
score: 19.381296
IPR004046Glutathione S-transferase, C-terminalPFAMPF00043GST_Ccoord: 218..303
e-value: 7.9E-11
score: 42.0
coord: 15..110
e-value: 2.8E-11
score: 43.5
IPR045073Glutathione S-transferase Omega/Tau-likeSFLDSFLDG01152Main.3:_Omega-_and_Tau-like_coord: 117..322
e-value: 0.0
score: 264.1
IPR040079Glutathione Transferase familySFLDSFLDS00019Glutathione_Transferase_(cytocoord: 117..322
e-value: 0.0
score: 264.1
IPR010987Glutathione S-transferase, C-terminal-likePROSITEPS50405GST_CTERcoord: 5..135
score: 16.463135
IPR010987Glutathione S-transferase, C-terminal-likePROSITEPS50405GST_CTERcoord: 198..321
score: 17.851498
IPR045074Glutathione S-transferases Tau, C-terminal alpha helical domain, plantCDDcd03185GST_C_Taucoord: 199..321
e-value: 1.37394E-29
score: 108.035
IPR045074Glutathione S-transferases Tau, C-terminal alpha helical domain, plantCDDcd03185GST_C_Taucoord: 6..117
e-value: 2.9935E-42
score: 140.777
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 187..323
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 2..121
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 117..194

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g20600.1Moc01g20600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006749 glutathione metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding