Moc01g20450 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g20450
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Laccase
Location	chr1: 14312035 .. 14313384 (-)
RNA-Seq Expression	Moc01g20450
Synteny	Moc01g20450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAACGTGTCGTTCGTGATCCCAAACGACGCCGGATTGTCGATGTTGGAGGCGTTTTTCCACCGGGTGGAGGGCGTTTATACGGCGGATTTTCCCGATCGGCCGCCGGTTGAATTCGATTACACGAATCCGAATATTTCTGTGGAGAGTCCGTTGATCTTCGCGCCGAAGGCCACGAAGGTGAAGAAATTGAAGTTCAATTCGACGGTGGAGATGGTTCTGCAGAACACTGCATTTATTGCGATGGGGAATCACCCATTGCATCTCCATCGATTTAACTTCCATGTTCTGGCTCAGGGATTCGGAAATTACGACCCGATTAACGACCCGAAGAAGTTCAATTTCTATAACCCGCAGATCCGGAACACCGTCGCCGTCCCCGTCGGCGGTTGGGCTGTCATCCGGTTCCAAGCCAACAATCCAGGTCCTCATTCTTTTCTTTTTTAATTTTACTCCCTTAAATTTTTTCACATTCATTTTATTTTTTTTAGATAAGAGAATATTTATCCTATTTTGGTACCTAATTTGTGATTTTAACCTTTGAAGTTTAAAGATAATCATTAAGGTCTGTATCTTTATTTTAACTAGAATATTGTGCATGTTAATTACATAGTGGACTAAGTCTGGATATATTAAATGGTAAACGAATAACAATTACCAAAATAATTGTTTTTTTTTTTAAGTTTGAGGATTAAAATTGATATCTTGAAAGTTAAAAGATGGAAATGGTTATTTTGTAAATTTAAAAAGAACACAATGTTTTTTAAAGTTAAAATTTTGAGTCTTTATAGTTGAACCGATTAAAACATATATCATTTTTATATAAAAAAAAAAGTTGAAAGTTTGAATTATTTCTACTCTCATATTATACTAAAAACAAACTTCAAGTGATAAGTTTAATAATTTAGTCTCAACTTAAAAACAAAATTACTTTAATAAAATTTTAAAATGCTATGTAATTAGCATGATCTAATAAAGTTTTCGACTTTTAATTTTATGTTTAATGAATTGCTACCTTTGTGATAGAAATTTATTATTTATTAGACACAAAAAGGATAAGTTTAGAAATTTATTTGAAATTTTTTAAAATATAGTTTTGGAATAGATGGATTGTAGATATAACCTAATTTATGACTAAACTTCTTTTTTATTGATAATATTACCAGTCCGTGATTAGTATTTTTTTTAACGGTTTAGGATGTATTGAATTTAACAGGTGTATGGCTGATGCACTGCCACCTGGATGTCCACCTACCGTGGGGATTAGCCATGGGGTTTGAAGTCGAGAACGGACCAGCTCCGTCGTCAAGGCTGCCTCCGCCGCCGCACGATCTTCCCAAATGCTAG

mRNA sequence

ATGAACAACGTGTCGTTCGTGATCCCAAACGACGCCGGATTGTCGATGTTGGAGGCGTTTTTCCACCGGGTGGAGGGCGTTTATACGGCGGATTTTCCCGATCGGCCGCCGGTTGAATTCGATTACACGAATCCGAATATTTCTGTGGAGAGTCCGTTGATCTTCGCGCCGAAGGCCACGAAGGTGAAGAAATTGAAGTTCAATTCGACGGTGGAGATGGTTCTGCAGAACACTGCATTTATTGCGATGGGGAATCACCCATTGCATCTCCATCGATTTAACTTCCATGTTCTGGCTCAGGGATTCGGAAATTACGACCCGATTAACGACCCGAAGAAGTTCAATTTCTATAACCCGCAGATCCGGAACACCGTCGCCGTCCCCGTCGGCGGTTGGGCTGTCATCCGGTTCCAAGCCAACAATCCAGGTGTATGGCTGATGCACTGCCACCTGGATGTCCACCTACCGTGGGGATTAGCCATGGGGTTTGAAGTCGAGAACGGACCAGCTCCGTCGTCAAGGCTGCCTCCGCCGCCGCACGATCTTCCCAAATGCTAG

Coding sequence (CDS)

ATGAACAACGTGTCGTTCGTGATCCCAAACGACGCCGGATTGTCGATGTTGGAGGCGTTTTTCCACCGGGTGGAGGGCGTTTATACGGCGGATTTTCCCGATCGGCCGCCGGTTGAATTCGATTACACGAATCCGAATATTTCTGTGGAGAGTCCGTTGATCTTCGCGCCGAAGGCCACGAAGGTGAAGAAATTGAAGTTCAATTCGACGGTGGAGATGGTTCTGCAGAACACTGCATTTATTGCGATGGGGAATCACCCATTGCATCTCCATCGATTTAACTTCCATGTTCTGGCTCAGGGATTCGGAAATTACGACCCGATTAACGACCCGAAGAAGTTCAATTTCTATAACCCGCAGATCCGGAACACCGTCGCCGTCCCCGTCGGCGGTTGGGCTGTCATCCGGTTCCAAGCCAACAATCCAGGTGTATGGCTGATGCACTGCCACCTGGATGTCCACCTACCGTGGGGATTAGCCATGGGGTTTGAAGTCGAGAACGGACCAGCTCCGTCGTCAAGGCTGCCTCCGCCGCCGCACGATCTTCCCAAATGCTAG

Protein sequence

MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKATKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPHDLPKC

Homology

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BLAST of Moc01g20450 vs. NCBI nr
Match: XP_022151516.1 (LOW QUALITY PROTEIN: laccase-7 [Momordica charantia])

HSP 1 Score: 399.4 bits (1025), Expect = 1.6e-107
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT
Sbjct: 415 MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 474

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
           KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ
Sbjct: 475 KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 534

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH
Sbjct: 535 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 594

Query: 181 DLPKC 186
           DLPKC
Sbjct: 595 DLPKC 599

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BLAST of Moc01g20450 vs. NCBI nr
Match: XP_038883561.1 (laccase-7-like [Benincasa hispida])

HSP 1 Score: 359.8 bits (922), Expect = 1.4e-95
Identity = 160/185 (86.49%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSFVIPNDAGLSMLEA+FH+VEGVY+ DFPD PPV+FDYTNP++ +++ LIFAPKAT
Sbjct: 387 MNNVSFVIPNDAGLSMLEAYFHKVEGVYSRDFPDDPPVKFDYTNPSLGLDNSLIFAPKAT 446

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
           KVKKLKFNSTVEM+LQNTAFI++ NHP+HLH FNFHVLAQGFGNYDPI DPKKFNF NPQ
Sbjct: 447 KVKKLKFNSTVEMILQNTAFISLENHPMHLHGFNFHVLAQGFGNYDPIRDPKKFNFVNPQ 506

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNT+AVPVGGWAVIRFQANNPGVWLMHCHLDVH+PWGLAMGFEVENGP PS+RLPPPPH
Sbjct: 507 IRNTIAVPVGGWAVIRFQANNPGVWLMHCHLDVHVPWGLAMGFEVENGPTPSTRLPPPPH 566

Query: 181 DLPKC 186
           DLPKC
Sbjct: 567 DLPKC 571

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BLAST of Moc01g20450 vs. NCBI nr
Match: XP_022978127.1 (laccase-7-like [Cucurbita maxima])

HSP 1 Score: 352.8 bits (904), Expect = 1.8e-93
Identity = 158/185 (85.41%), Postives = 174/185 (94.05%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSFVIPNDAGLSMLEAFFH+V+GVY+ DFPD+PPVEFDYTN +I +++ LIFAPKAT
Sbjct: 389 MNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEFDYTNSSIRLDNSLIFAPKAT 448

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
            VKKLKFNSTVEMVLQNTAF+++ NHP+HLH FNFHVLAQGFGNYDPI+DPKKFNF NPQ
Sbjct: 449 SVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQGFGNYDPIHDPKKFNFLNPQ 508

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNT+AVPV GWAVIRFQANNPGVWLMHCHLDVH+PWGLAMGFEVENGP PS+ LPPPPH
Sbjct: 509 IRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLAMGFEVENGPTPSTWLPPPPH 568

Query: 181 DLPKC 186
           DLPKC
Sbjct: 569 DLPKC 573

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BLAST of Moc01g20450 vs. NCBI nr
Match: KAG7033800.1 (Laccase-7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 352.8 bits (904), Expect = 1.8e-93
Identity = 159/185 (85.95%), Postives = 173/185 (93.51%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSFVIPNDAGLSMLEAFFH++EG+YT DFPD+PPVEFDYTN +I +++ LIFA KAT
Sbjct: 341 MNNVSFVIPNDAGLSMLEAFFHQLEGIYTGDFPDQPPVEFDYTNSSIRLDNSLIFAAKAT 400

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
            VKKLKFNSTVE+VLQNTAFIA  NHPLHLH FNFHVLAQGFGNYDPI+DP+KFNF NPQ
Sbjct: 401 SVKKLKFNSTVEIVLQNTAFIAKENHPLHLHGFNFHVLAQGFGNYDPIHDPEKFNFLNPQ 460

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNT+AVP+GGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEV NGP PS+RLPPPPH
Sbjct: 461 IRNTIAVPIGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVANGPTPSTRLPPPPH 520

Query: 181 DLPKC 186
           DLPKC
Sbjct: 521 DLPKC 525

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BLAST of Moc01g20450 vs. NCBI nr
Match: XP_023543234.1 (laccase-7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 351.7 bits (901), Expect = 3.9e-93
Identity = 157/185 (84.86%), Postives = 174/185 (94.05%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSFVIPNDAGLSMLEA+FH+V+GVY+ DFPD+PPVEFDYTN +I +++ LIFAPKAT
Sbjct: 369 MNNVSFVIPNDAGLSMLEAYFHKVKGVYSTDFPDQPPVEFDYTNSSIRLDNSLIFAPKAT 428

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
            VKKLKFNSTVEMVLQNTAF+++ NHP+HLH FNFHVLAQGFGNYDPI+DPKKFNF NPQ
Sbjct: 429 SVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQGFGNYDPIHDPKKFNFLNPQ 488

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNT+AVPV GWAVIRFQANNPGVWLMHCHLDVH+PWGLAMGFEVENGP PS+ LPPPPH
Sbjct: 489 IRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLAMGFEVENGPTPSTWLPPPPH 548

Query: 181 DLPKC 186
           DLPKC
Sbjct: 549 DLPKC 553

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BLAST of Moc01g20450 vs. ExPASy Swiss-Prot
Match: Q9SR40 (Laccase-7 OS=Arabidopsis thaliana OX=3702 GN=LAC7 PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.3e-70
Identity = 124/186 (66.67%), Postives = 148/186 (79.57%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESP-LIFAPKA 60
           M+N SFV+P    LS+LEA FH V+G++TADFPD+PPV+FDYTNPN++  +P L+F  K+
Sbjct: 384 MSNHSFVLPKK--LSILEAVFHDVKGIFTADFPDQPPVKFDYTNPNVTQTNPGLLFTQKS 443

Query: 61  TKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNP 120
           T  K LKFN+TVE+VLQN A IA  +HP+HLH FNFHVLAQGFGNYDP  D  K N  +P
Sbjct: 444 TSAKILKFNTTVEVVLQNHALIAAESHPMHLHGFNFHVLAQGFGNYDPSRDRSKLNLVDP 503

Query: 121 QIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPP 180
           Q RNT+AVPVGGWAVIRF ANNPG W+ HCH+DVHLP+GL M F V+NGP  S+ LPPPP
Sbjct: 504 QSRNTLAVPVGGWAVIRFTANNPGAWIFHCHIDVHLPFGLGMIFVVKNGPTKSTTLPPPP 563

Query: 181 HDLPKC 186
            DLPKC
Sbjct: 564 PDLPKC 567

[Close]

BLAST of Moc01g20450 vs. ExPASy Swiss-Prot
Match: Q2QUN2 (Laccase-24 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC24 PE=2 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 5.8e-63
Identity = 114/186 (61.29%), Postives = 135/186 (72.58%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFH-RVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKA 60
           MNN SF++PN    SMLEA +   ++GVYT DFPD PP+ FDYT       + L    K+
Sbjct: 396 MNNASFILPNTT--SMLEAMYKGSIDGVYTRDFPDTPPIVFDYTADASDDNATLKHTFKS 455

Query: 61  TKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNP 120
           TKVK LK+NSTV+MVLQNT  ++  +HP+HLH FNF VLAQGFGNY+   DP KFN  +P
Sbjct: 456 TKVKTLKYNSTVQMVLQNTRLVSKESHPMHLHGFNFFVLAQGFGNYNETTDPAKFNLVDP 515

Query: 121 QIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPP 180
           Q RNTVAVP GGWAVIRF A+NPGVW MHCH D HL +GL M FEV+NGP   + LPPPP
Sbjct: 516 QERNTVAVPTGGWAVIRFVADNPGVWFMHCHFDAHLEFGLGMVFEVQNGPTQETSLPPPP 575

Query: 181 HDLPKC 186
            DLP+C
Sbjct: 576 SDLPQC 579

[Close]

BLAST of Moc01g20450 vs. ExPASy Swiss-Prot
Match: Q9LFD2 (Laccase-8 OS=Arabidopsis thaliana OX=3702 GN=LAC8 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-61
Identity = 104/187 (55.61%), Postives = 139/187 (74.33%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYT--NPNISVESPLIFAPK 60
           +NN +F+IP    +SM EA+F+ + G+YT DFP++PP++FDYT      + +  ++F  +
Sbjct: 382 LNNRTFMIPE--RISMQEAYFYNISGIYTDDFPNQPPLKFDYTKFEQRTNNDMKMMFPER 441

Query: 61  ATKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYN 120
            T VKK++FNSTVE+VLQNTA I+  +HP+HLH FNF+VL  GFGNYDPI D +K N +N
Sbjct: 442 KTSVKKIRFNSTVEIVLQNTAIISPESHPMHLHGFNFYVLGYGFGNYDPIRDARKLNLFN 501

Query: 121 PQIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPP 180
           PQ+ NTV VP GGW V+RF ANNPGVWL HCH+D HLP+G+   F V+NGP P + LP P
Sbjct: 502 PQMHNTVGVPPGGWVVLRFIANNPGVWLFHCHMDAHLPYGIMSAFIVQNGPTPETSLPSP 561

Query: 181 PHDLPKC 186
           P +LP+C
Sbjct: 562 PSNLPQC 566

[Close]

BLAST of Moc01g20450 vs. ExPASy Swiss-Prot
Match: Q69L99 (Laccase-14 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC14 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 7.0e-61
Identity = 115/188 (61.17%), Postives = 138/188 (73.40%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTN-PNISVESPLIFAPKA 60
           MNNVSF +P  A +S+LEA   R  GVYT DFPDRPPV FD+TN   ++    L+   K 
Sbjct: 398 MNNVSFQLP--ATMSLLEASRSRSSGVYTRDFPDRPPVMFDFTNAAAVNRNMSLMVTSKG 457

Query: 61  TKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKF--NFY 120
           T+VK L++N TVE+VLQNTA +   NHPLHLH FNF+VLAQG GNY  +   KK   N  
Sbjct: 458 TRVKALRYNETVEVVLQNTAVLGTENHPLHLHGFNFYVLAQGTGNYYYLIRKKKIRKNLV 517

Query: 121 NPQIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPP 180
           NPQ RNT+AVP GGWAVIRF A+NPGVWLMHCHL+ HLP+GLAM F+V++GP P + LPP
Sbjct: 518 NPQQRNTIAVPPGGWAVIRFTADNPGVWLMHCHLEAHLPFGLAMAFDVQDGPTPDAMLPP 577

Query: 181 PPHDLPKC 186
           PP+D P C
Sbjct: 578 PPNDYPPC 583

[Close]

BLAST of Moc01g20450 vs. ExPASy Swiss-Prot
Match: Q5N7A3 (Laccase-6 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC6 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 7.8e-60
Identity = 114/188 (60.64%), Postives = 138/188 (73.40%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFF-HRVEGVYTADFPDRPPVEFDYTNPNISVESPLI-FAPK 60
           MNNVSF +PN   +SMLEA + +  +GVYT DFPD+PPV FDYT+  +   SPL      
Sbjct: 395 MNNVSFALPNT--VSMLEALYRNTADGVYTRDFPDQPPVAFDYTSRGLLGNSPLASTGSP 454

Query: 61  ATKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYD-PINDPKKFNFY 120
           +TKVK L++N+TVEMVLQNTA + + +HP+HLH FNF V+AQGFGN D       +FN  
Sbjct: 455 STKVKTLRYNATVEMVLQNTALVGLESHPMHLHGFNFFVVAQGFGNNDGEAAGAGEFNLV 514

Query: 121 NPQIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPP 180
           NPQ RNTVAVP GGWAVIRF A+NPG+W MHCH+D H   GLAM FEVE+GP P + LPP
Sbjct: 515 NPQERNTVAVPTGGWAVIRFVADNPGMWAMHCHIDSHFAIGLAMVFEVESGPTPGTTLPP 574

Query: 181 PPHDLPKC 186
           PP DLP+C
Sbjct: 575 PPPDLPQC 580

[Close]

BLAST of Moc01g20450 vs. ExPASy TrEMBL
Match: A0A6J1DEW8 (Laccase OS=Momordica charantia OX=3673 GN=LOC111019442 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 8.0e-108
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT
Sbjct: 415 MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 474

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
           KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ
Sbjct: 475 KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 534

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH
Sbjct: 535 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 594

Query: 181 DLPKC 186
           DLPKC
Sbjct: 595 DLPKC 599

[Close]

BLAST of Moc01g20450 vs. ExPASy TrEMBL
Match: A0A6J1IKA9 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111478200 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 8.5e-94
Identity = 158/185 (85.41%), Postives = 174/185 (94.05%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSFVIPNDAGLSMLEAFFH+V+GVY+ DFPD+PPVEFDYTN +I +++ LIFAPKAT
Sbjct: 389 MNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEFDYTNSSIRLDNSLIFAPKAT 448

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
            VKKLKFNSTVEMVLQNTAF+++ NHP+HLH FNFHVLAQGFGNYDPI+DPKKFNF NPQ
Sbjct: 449 SVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQGFGNYDPIHDPKKFNFLNPQ 508

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNT+AVPV GWAVIRFQANNPGVWLMHCHLDVH+PWGLAMGFEVENGP PS+ LPPPPH
Sbjct: 509 IRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLAMGFEVENGPTPSTWLPPPPH 568

Query: 181 DLPKC 186
           DLPKC
Sbjct: 569 DLPKC 573

[Close]

BLAST of Moc01g20450 vs. ExPASy TrEMBL
Match: A0A6J1GGH4 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453717 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.9e-93
Identity = 157/185 (84.86%), Postives = 174/185 (94.05%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSFVIPNDAGLSMLEA+FH+V+GVY+ DFPD+PPVEFDYTN +I +++ LIFAPKAT
Sbjct: 387 MNNVSFVIPNDAGLSMLEAYFHKVKGVYSTDFPDQPPVEFDYTNSSIRLDNSLIFAPKAT 446

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
            VKKLKFNSTVEMVLQNTAF+++ NHP+HLH FNFHVLAQGFGNYDPI+DPKKFNF NPQ
Sbjct: 447 SVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQGFGNYDPIHDPKKFNFLNPQ 506

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNT+AVPV GWAVIRFQANNPGVWLMHCHLDVH+PWGLAMGFEVENGP PS+ LPPPPH
Sbjct: 507 IRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLAMGFEVENGPTPSTWLPPPPH 566

Query: 181 DLPKC 186
           DLPKC
Sbjct: 567 DLPKC 571

[Close]

BLAST of Moc01g20450 vs. ExPASy TrEMBL
Match: A0A6J1GEQ7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453486 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.2e-92
Identity = 159/185 (85.95%), Postives = 170/185 (91.89%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           M NVSFVIPNDAGLSMLEAFFH+VEGVYT DFPD PPVEFDYTN +I +++ LIFA KAT
Sbjct: 384 MXNVSFVIPNDAGLSMLEAFFHKVEGVYTGDFPDLPPVEFDYTNSSIRLDNSLIFAAKAT 443

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
            VKKLKFNSTVE+VLQNTAFIA  NHPLHLH FNFHVLAQGFGNYDPI+DP+KFNF NPQ
Sbjct: 444 SVKKLKFNSTVEIVLQNTAFIAKENHPLHLHGFNFHVLAQGFGNYDPIHDPEKFNFLNPQ 503

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNT+AVP+GGW VIRFQANNPGVWLMHCHLDVHLPWGLAMGFEV NGP PS+RLPPPPH
Sbjct: 504 IRNTIAVPIGGWPVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVANGPTPSTRLPPPPH 563

Query: 181 DLPKC 186
           DLPKC
Sbjct: 564 DLPKC 568

[Close]

BLAST of Moc01g20450 vs. ExPASy TrEMBL
Match: A0A0A0L2I3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G306850 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.0e-91
Identity = 157/185 (84.86%), Postives = 169/185 (91.35%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSFVIPNDAGLSMLEA+FH+VEGVY+ DFPD P V+FDYTN ++ ++S LIFAPK T
Sbjct: 176 MNNVSFVIPNDAGLSMLEAYFHKVEGVYSRDFPDEPAVKFDYTNSSLGLDSSLIFAPKGT 235

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
           KVKKLKFNSTVE+VLQNTAFIA  NHPLHLH FNFHVLAQGFGNYDPI DP  FNF NPQ
Sbjct: 236 KVKKLKFNSTVEIVLQNTAFIAKENHPLHLHGFNFHVLAQGFGNYDPIRDPYMFNFVNPQ 295

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           IRNT+AVP+GGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGP PS+RLPPPP 
Sbjct: 296 IRNTIAVPIGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPTPSTRLPPPPF 355

Query: 181 DLPKC 186
           DLPKC
Sbjct: 356 DLPKC 360

[Close]

BLAST of Moc01g20450 vs. TAIR 10
Match: AT3G09220.1 (laccase 7 )

HSP 1 Score: 267.3 bits (682), Expect = 9.1e-72
Identity = 124/186 (66.67%), Postives = 148/186 (79.57%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESP-LIFAPKA 60
           M+N SFV+P    LS+LEA FH V+G++TADFPD+PPV+FDYTNPN++  +P L+F  K+
Sbjct: 384 MSNHSFVLPKK--LSILEAVFHDVKGIFTADFPDQPPVKFDYTNPNVTQTNPGLLFTQKS 443

Query: 61  TKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNP 120
           T  K LKFN+TVE+VLQN A IA  +HP+HLH FNFHVLAQGFGNYDP  D  K N  +P
Sbjct: 444 TSAKILKFNTTVEVVLQNHALIAAESHPMHLHGFNFHVLAQGFGNYDPSRDRSKLNLVDP 503

Query: 121 QIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPP 180
           Q RNT+AVPVGGWAVIRF ANNPG W+ HCH+DVHLP+GL M F V+NGP  S+ LPPPP
Sbjct: 504 QSRNTLAVPVGGWAVIRFTANNPGAWIFHCHIDVHLPFGLGMIFVVKNGPTKSTTLPPPP 563

Query: 181 HDLPKC 186
            DLPKC
Sbjct: 564 PDLPKC 567

[Close]

BLAST of Moc01g20450 vs. TAIR 10
Match: AT5G01040.1 (laccase 8 )

HSP 1 Score: 237.7 bits (605), Expect = 7.7e-63
Identity = 104/187 (55.61%), Postives = 139/187 (74.33%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYT--NPNISVESPLIFAPK 60
           +NN +F+IP    +SM EA+F+ + G+YT DFP++PP++FDYT      + +  ++F  +
Sbjct: 382 LNNRTFMIPE--RISMQEAYFYNISGIYTDDFPNQPPLKFDYTKFEQRTNNDMKMMFPER 441

Query: 61  ATKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYN 120
            T VKK++FNSTVE+VLQNTA I+  +HP+HLH FNF+VL  GFGNYDPI D +K N +N
Sbjct: 442 KTSVKKIRFNSTVEIVLQNTAIISPESHPMHLHGFNFYVLGYGFGNYDPIRDARKLNLFN 501

Query: 121 PQIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPP 180
           PQ+ NTV VP GGW V+RF ANNPGVWL HCH+D HLP+G+   F V+NGP P + LP P
Sbjct: 502 PQMHNTVGVPPGGWVVLRFIANNPGVWLFHCHMDAHLPYGIMSAFIVQNGPTPETSLPSP 561

Query: 181 PHDLPKC 186
           P +LP+C
Sbjct: 562 PSNLPQC 566

[Close]

BLAST of Moc01g20450 vs. TAIR 10
Match: AT5G01050.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 229.6 bits (584), Expect = 2.1e-60
Identity = 103/189 (54.50%), Postives = 137/189 (72.49%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYT----NPNISVESPLIFA 60
           +NN +F+IP    +SM EA+F+ + GVYT DFPD+PP++FD+T    +P  S +  ++F 
Sbjct: 383 LNNRTFMIPE--RISMQEAYFYNITGVYTDDFPDQPPLKFDFTKFEQHPTNS-DMEMMFP 442

Query: 61  PKATKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNF 120
            + T VK ++FNSTVE+VLQNT  +   +HP+HLH FNF+VL  GFGNYDPI D +K N 
Sbjct: 443 ERKTSVKTIRFNSTVEIVLQNTGILTPESHPMHLHGFNFYVLGYGFGNYDPIRDARKLNL 502

Query: 121 YNPQIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLP 180
           +NPQ+ NTV VP GGW V+RF ANNPG+WL HCH+D HLP G+ M F V+NGP   + LP
Sbjct: 503 FNPQMHNTVGVPPGGWVVLRFIANNPGIWLFHCHMDAHLPLGIMMAFIVQNGPTRETSLP 562

Query: 181 PPPHDLPKC 186
            PP +LP+C
Sbjct: 563 SPPSNLPQC 568

[Close]

BLAST of Moc01g20450 vs. TAIR 10
Match: AT5G05390.1 (laccase 12 )

HSP 1 Score: 225.7 bits (574), Expect = 3.0e-59
Identity = 105/186 (56.45%), Postives = 136/186 (73.12%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAP-KA 60
           MNNVSFV+P++   S+L+A  + + GV+T DFP +PPV+FDYT  NI   S  +F P K 
Sbjct: 385 MNNVSFVLPSN--FSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNI---SRALFQPVKG 444

Query: 61  TKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNP 120
           TK+ KLK+ S V++VLQ+T  +   NHP+HLH ++F+++ +GFGN++P  D  KFN  +P
Sbjct: 445 TKLYKLKYGSRVQVVLQDTNIVTSENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDP 504

Query: 121 QIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPP 180
            +RNTVAVPV GWAVIRF A+NPGVWLMHCHLDVH+ WGLAM F V+NG      L  PP
Sbjct: 505 PLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKWGLAMAFLVDNGVGELETLEAPP 564

Query: 181 HDLPKC 186
           HDLP C
Sbjct: 565 HDLPIC 565

[Close]

BLAST of Moc01g20450 vs. TAIR 10
Match: AT2G40370.1 (laccase 5 )

HSP 1 Score: 221.9 bits (564), Expect = 4.4e-58
Identity = 101/185 (54.59%), Postives = 132/185 (71.35%), Query Frame = 0


Query: 1   MNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFDYTNPNISVESPLIFAPKAT 60
           MNNVSF +P++   S+L+A  H + GV+T DFP +PPV+FDYT  NIS    L    + T
Sbjct: 400 MNNVSFALPSN--YSLLQAHHHGIPGVFTTDFPAKPPVKFDYTGNNIS--RSLYQPDRGT 459

Query: 61  KVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQGFGNYDPINDPKKFNFYNPQ 120
           K+ KLK+ S V++VLQ+T  +   NHP+HLH ++F+++A+GFGN++P  D  KFN  +P 
Sbjct: 460 KLYKLKYGSRVQIVLQDTGIVTPENHPIHLHGYDFYIIAEGFGNFNPKKDTAKFNLEDPP 519

Query: 121 IRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAMGFEVENGPAPSSRLPPPPH 180
           +RNTV VPV GWAVIRF A+NPGVW+MHCHLD H+ WGLAM F VENG      +  PPH
Sbjct: 520 LRNTVGVPVNGWAVIRFIADNPGVWIMHCHLDAHISWGLAMAFLVENGNGVLQTIEQPPH 579

Query: 181 DLPKC 186
           DLP C
Sbjct: 580 DLPVC 580

The following BLAST results are available for this feature:

BLAST of Moc01g20450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151516.1	1.6e-107	100.00	LOW QUALITY PROTEIN: laccase-7 [Momordica charantia]	[more]
XP_038883561.1	1.4e-95	86.49	laccase-7-like [Benincasa hispida]	[more]
XP_022978127.1	1.8e-93	85.41	laccase-7-like [Cucurbita maxima]	[more]
KAG7033800.1	1.8e-93	85.95	Laccase-7, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023543234.1	3.9e-93	84.86	laccase-7 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc01g20450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SR40	1.3e-70	66.67	Laccase-7 OS=Arabidopsis thaliana OX=3702 GN=LAC7 PE=2 SV=1	[more]
Q2QUN2	5.8e-63	61.29	Laccase-24 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC24 PE=2 SV=1	[more]
Q9LFD2	1.1e-61	55.61	Laccase-8 OS=Arabidopsis thaliana OX=3702 GN=LAC8 PE=2 SV=1	[more]
Q69L99	7.0e-61	61.17	Laccase-14 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC14 PE=3 SV=1	[more]
Q5N7A3	7.8e-60	60.64	Laccase-6 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC6 PE=2 SV=1	[more]

BLAST of Moc01g20450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DEW8	8.0e-108	100.00	Laccase OS=Momordica charantia OX=3673 GN=LOC111019442 PE=3 SV=1	[more]
A0A6J1IKA9	8.5e-94	85.41	Laccase OS=Cucurbita maxima OX=3661 GN=LOC111478200 PE=3 SV=1	[more]
A0A6J1GGH4	1.9e-93	84.86	Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453717 PE=3 SV=1	[more]
A0A6J1GEQ7	1.2e-92	85.95	Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453486 PE=3 SV=1	[more]
A0A0A0L2I3	1.0e-91	84.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G306850 PE=4 SV=1	[more]

BLAST of Moc01g20450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09220.1	9.1e-72	66.67	laccase 7	[more]
AT5G01040.1	7.7e-63	55.61	laccase 8	[more]
AT5G01050.1	2.1e-60	54.50	Laccase/Diphenol oxidase family protein	[more]
AT5G05390.1	3.0e-59	56.45	laccase 12	[more]
AT2G40370.1	4.4e-58	54.59	laccase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 1..185 e-value: 1.1E-52 score: 180.8
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 2..185
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 32..167 e-value: 1.2E-39 score: 135.2
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 1..185
None	No IPR available	PANTHER	PTHR11709:SF9	LACCASE-7	coord: 1..185
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 148..159
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 143..163

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g20450.1	Moc01g20450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046274	lignin catabolic process
cellular_component	GO:0048046	apoplast
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0052716	hydroquinone:oxygen oxidoreductase activity
molecular_function	GO:0016491	oxidoreductase activity