Moc01g17000 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g17000
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionEf-hand calcium binding protein
Locationchr1: 11408191 .. 11410723 (+)
RNA-Seq ExpressionMoc01g17000
SyntenyMoc01g17000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGGTTACCCAAATCAGCCTTCCGGTTACGGTTACGGCTACGGCGGCGCCGGAGCCGGCGGTGGATACGGAGGCTACGGAGCTCCTCCGCCAACTGCCCAGCCCTACGCCTCTTCGGCGTACGGCGGACAACAACCACCGCCTCACTCTCAGCAGTCGTACGCTCCGGTGTCCGCCCCGTACGGCGCACCGTCGGCACCGTACGGATCATCTACCGGAGACAAACCTCCGAAGGACAAGCCTCATCAGTCCTACGGCGGGGCTGGTGGCGGTCATCAATCTTACGGTGGAGCCGGTGGCGGTCATGCTTATCCTCCACCTAATTCGTACGGAAACCCTTTCGCGTCGCTCTTGCCCTCGGCCTTCCCGCCTGGTACTGATCCGAGCATCGTCGCCTGCTTCCAAGTAGCCGATCAGGATGGGAGTGGCTTTATCGATGATAAGGAGTTGCAAGGTGTTCTGTCCTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCACCTCCTCATGTACCATTTCACCAACACGAACACCAGGAAGATCGGTAATCGCACTTTCTCTTTATCACTTTTTCGTTTGCCCTGGAAATACGAAATTTCACGTCGTTTTAGTTTCTTAAATCAACAACAGTAATGATAAGATCCGGAAATTTCTGCTACATGCAAGTCATTAGTTCCAACATAGACGATGAGACCGTATCACATCACTTCAGCAAATTTTAATTGAAAGATAAACTGACAAAGATAAATGTATGTTCATAGAATAGGTCATCGGTTCATTATCAAAATTGTCGAGGTGAATTTCATGTAATCGCGTTGAATTGTAATGTTTGTTTTTAGTAATTATGATTATTTTTATAAAATTACTATTAGTTGATTAGTTCATGGTGCTCATACCTGTGGGGTATTTACTAGAAGATGCCAAATACGTTCTATTTATATTTCTGTTAATCCTATACTAAGATACCTCGGCTCAATCTTATTGCCTGCGATTAGTTAATGTTATTTCATGCTCTATTTTACATTTGTCTGGTCTGGTGGTTAGGTTAAGCAGTTGGGTTAATTACTCAAAATTTATGTAGAAAATTTAGAACCTACCATTTGGACTTGGATTAGTTTTGGTGGTTGCACGACTTTCACTATATTCAAATTGAGCAGTTGAAACTGTAGACTTCTCAAAACTCTGAACCTTTTGGGTTATTTGAGTGTCTGTCATTATGCAAGTATTTTTTTAAATGATAAAATGGTGTGGTATTTTGAATCAGAGCATATGGTTATATTGTCAATTTTTTAATCTGTTCGAATCATCTTACTCTTTTATGTATTTAAACCTCGAGTGGGGTGGGGGGTGCTGCTATAGATTTGATATGTGCTGTCTTGTGAATTGATCTCTAAATCATGATTTTACCTTGTCTATTGACATTGCTTTTCTATTTCTTTTCCTGAAAAACAGGGCCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCAGAGCTGGAGGGTAAGCTGAATATTTGATATTATTTGAAGCAAAAATTATTTGTTGGTGTAATTCTGTGAGATTGAGTATCTATTGTATCTTTTTCAGGGTATTTTTGAGAGATTCGATTCAGATAGAAGTGGAAAAATCGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCGGTGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGCGGTGGGAAAAGCAAGGCCATTGAATATGATAATTTCATAGAGTATGTTTGCCTTCCTTACTGTTGATCTGATGTGGTACTCTTTTTCTGATGAGGTTTCTTGTGATATTTAAGTGTTAAGAATTCTTTCGATCACAGTTCTTATTGTTTTCTTTTTGCAAATTCAGGTGCTGTCTTACGGTCAAGGTGAGCTTCTTCTGATTTGATTTGCATGTCTTCTAATTAGACTGATTAATATGAATATCTTTTACATATCACTGTATACTGTTTAGAGGTGATGGCATCATCTTTGAATCCACAGGCTTAGTGTGCAATCTGTTTAACTATTTGAGCATATTTGCCTACTTGTCATTACATCTACTGCTTAACCTTGAACTTGATTTTATTCCAACTCCTGTTGGTGCATGTGAATACACCGAACACCTGTTACACGAACCTGCATTTAACAAGCCTGTGGATCTAGTAGTCGCTTGTCATCTAATTTCACTATGTAAAATTGTTCTTGTTTTTATCCTTTTTCCCTTTCCTGTAACATGAAATGTTTTTTCACACACTGGTATTTTTAACACCTTGTATGAATTGAACCCGAGTCCTCGGCATCACATCTGCGTTGATATTTACGGCTCTCATAATCGTTTCTGTACCATGGCGACAAAGAAAGAGCTAAAACTAAAATGGTTTTGTTGTAAAATTTTTTTTTTACAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATATTCTGGTTCTGCAACCTTCAGTTACGAGGCTTTCATGCTGACTGTCCTGCCATTCCTTATCGCCTGA

mRNA sequence

ATGTCGGGTTACCCAAATCAGCCTTCCGGTTACGGTTACGGCTACGGCGGCGCCGGAGCCGGCGGTGGATACGGAGGCTACGGAGCTCCTCCGCCAACTGCCCAGCCCTACGCCTCTTCGGCGTACGGCGGACAACAACCACCGCCTCACTCTCAGCAGTCGTACGCTCCGGTGTCCGCCCCGTACGGCGCACCGTCGGCACCGTACGGATCATCTACCGGAGACAAACCTCCGAAGGACAAGCCTCATCAGTCCTACGGCGGGGCTGGTGGCGGTCATCAATCTTACGGTGGAGCCGGTGGCGGTCATGCTTATCCTCCACCTAATTCGTACGGAAACCCTTTCGCGTCGCTCTTGCCCTCGGCCTTCCCGCCTGGTACTGATCCGAGCATCGTCGCCTGCTTCCAAGTAGCCGATCAGGATGGGAGTGGCTTTATCGATGATAAGGAGTTGCAAGGTGTTCTGTCCTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCACCTCCTCATGTACCATTTCACCAACACGAACACCAGGAAGATCGGGCCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCAGAGCTGGAGGGGTATTTTTGAGAGATTCGATTCAGATAGAAGTGGAAAAATCGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCGGTGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGCGGTGGGAAAAGCAAGGCCATTGAATATGATAATTTCATAGAGTGCTGTCTTACGGTCAAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATATTCTGGTTCTGCAACCTTCAGTTACGAGGCTTTCATGCTGACTGTCCTGCCATTCCTTATCGCCTGA

Coding sequence (CDS)

ATGTCGGGTTACCCAAATCAGCCTTCCGGTTACGGTTACGGCTACGGCGGCGCCGGAGCCGGCGGTGGATACGGAGGCTACGGAGCTCCTCCGCCAACTGCCCAGCCCTACGCCTCTTCGGCGTACGGCGGACAACAACCACCGCCTCACTCTCAGCAGTCGTACGCTCCGGTGTCCGCCCCGTACGGCGCACCGTCGGCACCGTACGGATCATCTACCGGAGACAAACCTCCGAAGGACAAGCCTCATCAGTCCTACGGCGGGGCTGGTGGCGGTCATCAATCTTACGGTGGAGCCGGTGGCGGTCATGCTTATCCTCCACCTAATTCGTACGGAAACCCTTTCGCGTCGCTCTTGCCCTCGGCCTTCCCGCCTGGTACTGATCCGAGCATCGTCGCCTGCTTCCAAGTAGCCGATCAGGATGGGAGTGGCTTTATCGATGATAAGGAGTTGCAAGGTGTTCTGTCCTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCACCTCCTCATGTACCATTTCACCAACACGAACACCAGGAAGATCGGGCCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCAGAGCTGGAGGGGTATTTTTGAGAGATTCGATTCAGATAGAAGTGGAAAAATCGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCGGTGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGCGGTGGGAAAAGCAAGGCCATTGAATATGATAATTTCATAGAGTGCTGTCTTACGGTCAAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATATTCTGGTTCTGCAACCTTCAGTTACGAGGCTTTCATGCTGACTGTCCTGCCATTCCTTATCGCCTGA

Protein sequence

MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSAPYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Homology
BLAST of Moc01g17000 vs. NCBI nr
Match: XP_022153892.1 (probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_022153893.1 probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_022153894.1 probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_022153895.1 probable calcium-binding protein CML49 isoform X1 [Momordica charantia])

HSP 1 Score: 587.0 bits (1512), Expect = 8.9e-164
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA
Sbjct: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120
           PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP
Sbjct: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 297

BLAST of Moc01g17000 vs. NCBI nr
Match: XP_022966416.1 (probable calcium-binding protein CML49 [Cucurbita maxima])

HSP 1 Score: 519.2 bits (1336), Expect = 2.3e-143
Identity = 266/297 (89.56%), Postives = 275/297 (92.59%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYGGA      GGYGAPPPTAQPY SS YG Q PPPHSQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGA------GGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120
           PYGAPSAPYGS+ G+KP KDKP+QS+GG GG HQSY    GGH YPPP++YG+PFASLLP
Sbjct: 61  PYGAPSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSY----GGHGYPPPSAYGSPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKID NELREALLSLGFAVSP+VLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

BLAST of Moc01g17000 vs. NCBI nr
Match: XP_023518609.1 (probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.1 bits (1333), Expect = 5.1e-143
Identity = 265/297 (89.23%), Postives = 275/297 (92.59%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYGGA      GGYGAPPPTAQPY SS YG Q PPPHSQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGA------GGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120
           PYGAPSAPYGS+ G+KP KDKP+QS+GG GG HQSY    GGH YPPP++YG+PFASLLP
Sbjct: 61  PYGAPSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSY----GGHGYPPPSAYGSPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240
           KIGPKEFIALFYGLQSWRGIFERFDSDRSG+ID NELREALLSLGFAVSP+VLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

BLAST of Moc01g17000 vs. NCBI nr
Match: XP_004142653.1 (probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetical protein Csa_011963 [Cucumis sativus])

HSP 1 Score: 515.4 bits (1326), Expect = 3.3e-142
Identity = 267/298 (89.60%), Postives = 277/298 (92.95%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYG        GGYGAPPPTAQPY SSAYG   P   +QQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGST------GGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGG-AGGGHAYPPPNSYGNPFASLL 120
           PYG PSAPYGS+ G+KPPKDKP+QSYGGAGGGHQSYGG A GGH YPPP++YG+PFASLL
Sbjct: 61  PYGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLL 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSK 240
           RKIGPKEFI+LFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSP+VLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of Moc01g17000 vs. NCBI nr
Match: XP_038883807.1 (probable calcium-binding protein CML50 [Benincasa hispida])

HSP 1 Score: 515.0 bits (1325), Expect = 4.3e-142
Identity = 270/298 (90.60%), Postives = 279/298 (93.62%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYG     GG GGYGAPPPTAQ Y SSAYG  Q P  +QQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYG----YGGSGGYGAPPPTAQVYNSSAYGHPQ-PQQTQQPYAPVAA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAG-GGHAYPPPNSYGNPFASLL 120
           PYGAPSAPYGS+ G+KPPKDKP+QSYGGAGGGHQSYGGAG GGH YPPP++YGNPFASLL
Sbjct: 61  PYGAPSAPYGSAPGEKPPKDKPNQSYGGAGGGHQSYGGAGSGGHGYPPPSAYGNPFASLL 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQ VLSSY+QKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQRVLSSYDQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSK 240
           RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDS ELREALLSLGFAVSP+VLDLLVSK
Sbjct: 181 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSKELREALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 293

BLAST of Moc01g17000 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.2e-100
Identity = 220/366 (60.11%), Postives = 242/366 (66.12%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYP    GYGYGYGG            PPP   PY+S   GG  PP  S  + +P + 
Sbjct: 1   MSGYPPTSQGYGYGYGGG---------NQPPPPQPPYSS---GGNNPPYGSSTTSSPYAV 60

Query: 61  PYGA----------------------PSAPYGSSTGD--KPPKDKP-------------- 120
           PYGA                      PSAPY  S GD  KPPK+KP              
Sbjct: 61  PYGASKPQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSS 120

Query: 121 -HQSYG---------GAGGG-----------HQSYGGA---------GGGH-AYPPPNSY 180
            + SYG         G GGG           + SYGGA         GGG+  YPP  SY
Sbjct: 121 DYGSYGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASY 180

Query: 181 GNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLM 240
           G+PFASL+PS F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLM
Sbjct: 181 GSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLM 240

Query: 241 YHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPV 298
           Y FTN+N  KIGPKEF ALFY LQ+WR IFER D DRSG+ID NELR+ALLSLGF+VSPV
Sbjct: 241 YLFTNSNAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPV 300

BLAST of Moc01g17000 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.2e-99
Identity = 213/340 (62.65%), Postives = 238/340 (70.00%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 61  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 120
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 121 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 180
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 181 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSW 240
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+W
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNW 240

Query: 241 RGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIE 298
           R IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 300

BLAST of Moc01g17000 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 1.6e-94
Identity = 201/309 (65.05%), Postives = 229/309 (74.11%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPT--AQPYASSAYGGQQPPPHSQQSYAPV 60
           M+GYP  P G GY YGGA      GGYGAPPP   + P  S+   G +PP   + S +  
Sbjct: 1   MAGYPPNP-GSGYPYGGA------GGYGAPPPPYGSSPAPSAPPYGAKPPKEGKTSSS-- 60

Query: 61  SAPYGAPSAPYGSSTGDKPPKDKPHQSYGGAGG--GHQSYGGAGGGHAYPPPNS------ 120
           SAPY      YG+     PP  +P+ S GG G     Q YG   G    PPP+S      
Sbjct: 61  SAPYYGGGGGYGA-----PPSTQPYGSGGGYGAPPSSQPYGAPYGA---PPPSSAPYGAP 120

Query: 121 --YGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVH 180
             YG+PFASL+PSAFPPGTDP++VACFQ AD+DGSG IDDKELQ  LS Y+Q FS+RTVH
Sbjct: 121 GGYGSPFASLVPSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVH 180

Query: 181 LLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAV 240
           LLMY FTNTN RKIGPKEF ++FY LQ+WR IFERFD DRSGKID+ ELR+ALLSLG++V
Sbjct: 181 LLMYLFTNTNVRKIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATELRDALLSLGYSV 240

Query: 241 SPVVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 298
           SP VLDLLVSKFDK+GGK+KAIEYDNFIECCLTVKGLTEKFKEKDT +SGSATF+YEAFM
Sbjct: 241 SPTVLDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFSGSATFTYEAFM 292

BLAST of Moc01g17000 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 2.8e-35
Identity = 83/196 (42.35%), Postives = 120/196 (61.22%), Query Frame = 0

Query: 103 HAYPPPNSYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLS-SYNQK 162
           + Y  P+       S     F P T P IV  F+ AD++ SGF+++ EL+  LS S    
Sbjct: 29  YTYAYPSYQPTQQFSSYSGMFSPETHPEIVRSFESADRNRSGFLEESELRQALSLSGYDG 88

Query: 163 FSIRTVHLLM--YHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELRE 222
            S RT+ LL+  Y     +  ++GPKE++ L+  L  WR IF R+D DRSGK++S +LR+
Sbjct: 89  ISNRTIRLLLFIYKIPVDSLLRLGPKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRD 148

Query: 223 ALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGS 282
           A  +LG  +   V  L+VS+FD   GK+  + +D+F+EC + VKGLTEKF+E D  Y+G 
Sbjct: 149 AFYNLGCVLPTSVHQLIVSQFDDGTGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGY 208

Query: 283 ATFSYEAFMLTVLPFL 296
           AT SY+ FML V+PF+
Sbjct: 209 ATLSYDVFMLMVIPFI 224

BLAST of Moc01g17000 vs. ExPASy Swiss-Prot
Match: Q5PQ53 (Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.6e-27
Identity = 101/296 (34.12%), Postives = 147/296 (49.66%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPP--PTAQPYASSAYGGQQPPPHSQQSYAPV 60
           M+ YP     YG GY GA         G PP  P    Y    YGG   P  S    AP 
Sbjct: 1   MASYP-----YGQGYHGAA--------GQPPGAPQTNYYGGQQYGGGVQPAASYGRPAP- 60

Query: 61  SAPYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGN----P 120
            APYG+P  P G   G   P      + GG  GG       GG ++ P    YG+     
Sbjct: 61  GAPYGSP--PSGGVYGHPVPGSAAPGAPGGPYGGQ----APGGPYSVPGSTPYGSQQHGS 120

Query: 121 FASLLPSA-FPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYN-QKFSIRTVHLLMY 180
           +    P+   PPG DP   + FQ  D D SG+I  KEL+  L + N   F+  T  ++M 
Sbjct: 121 YGQGAPAGNIPPGVDPEAFSWFQTVDTDHSGYISLKELKQALVNTNWSSFNDETCTMMMN 180

Query: 181 HFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVV 240
            F  +N+ +I    F AL+  +Q WR +F+++D DRSG I+  EL +AL  +G+ +SP  
Sbjct: 181 MFDKSNSGRIDMFGFSALWRFIQQWRNLFQQYDRDRSGSINQGELHQALCQMGYQLSPQF 240

Query: 241 LDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 289
           + +++S++ +   +   ++ D FI+ C  ++ +TE F+EKDT   G+A  SYE F+
Sbjct: 241 VQIVMSRYAQRSAQ-PGLQLDRFIQICTQLQSMTEAFREKDTGQIGTAKLSYEDFI 275

BLAST of Moc01g17000 vs. ExPASy TrEMBL
Match: A0A6J1DIS2 (probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021303 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.3e-164
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA
Sbjct: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120
           PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP
Sbjct: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 297

BLAST of Moc01g17000 vs. ExPASy TrEMBL
Match: A0A6J1HP98 (probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC111466073 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.1e-143
Identity = 266/297 (89.56%), Postives = 275/297 (92.59%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYGGA      GGYGAPPPTAQPY SS YG Q PPPHSQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGA------GGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120
           PYGAPSAPYGS+ G+KP KDKP+QS+GG GG HQSY    GGH YPPP++YG+PFASLLP
Sbjct: 61  PYGAPSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSY----GGHGYPPPSAYGSPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKID NELREALLSLGFAVSP+VLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

BLAST of Moc01g17000 vs. ExPASy TrEMBL
Match: A0A0A0L336 (Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.6e-142
Identity = 267/298 (89.60%), Postives = 277/298 (92.95%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYG        GGYGAPPPTAQPY SSAYG   P   +QQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGST------GGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGG-AGGGHAYPPPNSYGNPFASLL 120
           PYG PSAPYGS+ G+KPPKDKP+QSYGGAGGGHQSYGG A GGH YPPP++YG+PFASLL
Sbjct: 61  PYGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLL 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSK 240
           RKIGPKEFI+LFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSP+VLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of Moc01g17000 vs. ExPASy TrEMBL
Match: A0A6J1EBH1 (probable calcium-binding protein CML49 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432514 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.7e-142
Identity = 263/297 (88.55%), Postives = 274/297 (92.26%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYGGA      GGYGAPPPTAQPY SS YG Q PPPHSQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGA------GGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120
           PYGAPSAPYGS+  +KP KDKP+QS+GG GG HQSY    GGH YPPP++YG+PFASLLP
Sbjct: 61  PYGAPSAPYGSNPSEKPHKDKPNQSHGGVGGSHQSY----GGHGYPPPSAYGSPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180
           SAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQ+FSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQRFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKID NELREALLSLGF+VSP+VLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFSVSPMVLDLLVSKF 240

Query: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

BLAST of Moc01g17000 vs. ExPASy TrEMBL
Match: A0A5D3BYW0 (Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00390 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 6.1e-142
Identity = 269/298 (90.27%), Postives = 279/298 (93.62%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYPNQPSGYGYGYG     GG GGYGAPPPTAQPY SSAYG   P   +QQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYG----YGGTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAA 60

Query: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGG-AGGGHAYPPPNSYGNPFASLL 120
           PYG PSAPY S+ G+KPPKDKP+QSYGGAGGGHQSYGG AGGGH YPPP++YGNPFASLL
Sbjct: 61  PYGGPSAPY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASLL 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSK 240
           RKIGPKEFI+LFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSP+VLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 298
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

BLAST of Moc01g17000 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 366.3 bits (939), Expect = 2.3e-101
Identity = 220/366 (60.11%), Postives = 242/366 (66.12%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYP    GYGYGYGG            PPP   PY+S   GG  PP  S  + +P + 
Sbjct: 1   MSGYPPTSQGYGYGYGGG---------NQPPPPQPPYSS---GGNNPPYGSSTTSSPYAV 60

Query: 61  PYGA----------------------PSAPYGSSTGD--KPPKDKP-------------- 120
           PYGA                      PSAPY  S GD  KPPK+KP              
Sbjct: 61  PYGASKPQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSS 120

Query: 121 -HQSYG---------GAGGG-----------HQSYGGA---------GGGH-AYPPPNSY 180
            + SYG         G GGG           + SYGGA         GGG+  YPP  SY
Sbjct: 121 DYGSYGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASY 180

Query: 181 GNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLM 240
           G+PFASL+PS F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLM
Sbjct: 181 GSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLM 240

Query: 241 YHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPV 298
           Y FTN+N  KIGPKEF ALFY LQ+WR IFER D DRSG+ID NELR+ALLSLGF+VSPV
Sbjct: 241 YLFTNSNAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPV 300

BLAST of Moc01g17000 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 364.4 bits (934), Expect = 8.8e-101
Identity = 213/340 (62.65%), Postives = 238/340 (70.00%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 61  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 120
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 121 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 180
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 181 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSW 240
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+W
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNW 240

Query: 241 RGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIE 298
           R IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 300

BLAST of Moc01g17000 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 297.0 bits (759), Expect = 1.7e-80
Identity = 179/300 (59.67%), Postives = 202/300 (67.33%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 61  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 120
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 121 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 180
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 181 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSW 240
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+W
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNW 240

Query: 241 RGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIE 258
           R IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

BLAST of Moc01g17000 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 297.0 bits (759), Expect = 1.7e-80
Identity = 179/300 (59.67%), Postives = 202/300 (67.33%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 61  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 120
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 121 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 180
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 181 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSW 240
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+W
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNW 240

Query: 241 RGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIE 258
           R IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

BLAST of Moc01g17000 vs. TAIR 10
Match: AT3G10300.5 (Calcium-binding EF-hand family protein )

HSP 1 Score: 292.4 bits (747), Expect = 4.2e-79
Identity = 179/301 (59.47%), Postives = 202/301 (67.11%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 61  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 120
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 121 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 180
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 181 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIG-PKEFIALFYGLQS 240
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIG PKEF +LF+ LQ+
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGRPKEFTSLFFSLQN 240

Query: 241 WRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFI 258
           WR IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFI
Sbjct: 241 WRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFI 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153892.18.9e-164100.00probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_0221... [more]
XP_022966416.12.3e-14389.56probable calcium-binding protein CML49 [Cucurbita maxima][more]
XP_023518609.15.1e-14389.23probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo][more]
XP_004142653.13.3e-14289.60probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetica... [more]
XP_038883807.14.3e-14290.60probable calcium-binding protein CML50 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FYE43.2e-10060.11Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ... [more]
Q8W4L01.2e-9962.65Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ... [more]
Q2QY101.6e-9465.05Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2... [more]
Q9ZQH12.8e-3542.35Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ... [more]
Q5PQ531.6e-2734.12Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DIS24.3e-164100.00probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673... [more]
A0A6J1HP981.1e-14389.56probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC1114660... [more]
A0A0A0L3361.6e-14289.60Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 ... [more]
A0A6J1EBH12.7e-14288.55probable calcium-binding protein CML49 isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A5D3BYW06.1e-14290.27Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT5G04170.12.3e-10160.11Calcium-binding EF-hand family protein [more]
AT3G10300.38.8e-10162.65Calcium-binding EF-hand family protein [more]
AT3G10300.21.7e-8059.67Calcium-binding EF-hand family protein [more]
AT3G10300.41.7e-8059.67Calcium-binding EF-hand family protein [more]
AT3G10300.54.2e-7959.47Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 196..224
e-value: 0.0097
score: 25.1
coord: 130..158
e-value: 0.092
score: 21.9
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 131..154
e-value: 6.0E-5
score: 22.4
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 197..225
e-value: 2.3E-5
score: 23.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 192..227
score: 12.867577
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 126..161
score: 10.691735
NoneNo IPR availableGENE3D1.10.238.10coord: 104..296
e-value: 1.2E-39
score: 137.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..105
NoneNo IPR availablePANTHERPTHR46824:SF1CALCIUM-BINDING PROTEIN CML49-RELATEDcoord: 1..297
NoneNo IPR availableCDDcd16180EFh_PEF_Group_Icoord: 130..294
e-value: 2.65071E-76
score: 228.182
IPR044590Probable calcium-binding protein CML48/49/50PANTHERPTHR46824CALCIUM-BINDING PROTEIN CML48-RELATEDcoord: 1..297
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 139..151
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 205..217
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 125..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g17000.1Moc01g17000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding