Moc01g15770 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g15770
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionE3 ubiquitin-protein ligase RNF181-like
Locationchr1: 10143209 .. 10143850 (-)
RNA-Seq ExpressionMoc01g15770
SyntenyMoc01g15770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTTCGATACTTCCTCCACCGTCCGCTTCTGTCTCCACGGCGGCGCCGCCGCGGTTTCCTCCGGCGATCAGCCGCATCAGGTCCTGGAAGTCCGATTGAAGCGTCTGAGCCGTCTGATATCGCGATCTCCAGCGGCGGGGGTTCCGCACCAGGTCCTCCAGGAAACTCCGCCGTCTCCGGTGGCGGACGCCGCGTTCGTTCTCCCTCTGCGCGATCCGCTGTTTCTCGAGGCCTACGTCCGGCAGTTGCTGTGCTCGCTGAATCTCCCCGCCGGCGCCTGCGGAGCCATCGGCCGGAGGATCGCGTCGTTCGTCGTCGAATTGGCGGGCGGAAACAGTGCGGCGAGCCTCCACATTGTGGCGTTGGTGAATTACATTGAGGAGTTTTGGATGGATCCGATCCCGGCGCCGGTGCCACCGAGCAGAGGGGCGGCGGAATCGGCGATCTCGAGGCTGAAGAAGGAGAAGGTTGAAGAAGGAGTAGAATTGGGAGATTGCAGCGTTTGTTTGAATGAGATGAATGGCGGGAGGCAGGTGATTAGGATTCCGTGCGGTCATGTGTATCATGAATCCTGCATCCTCACATGGCTGAGGAAGAGCAATTCTTGCCCATTGTGTAGAACAAAGTTGGAACAATGA

mRNA sequence

ATGCCTTTCGATACTTCCTCCACCGTCCGCTTCTGTCTCCACGGCGGCGCCGCCGCGGTTTCCTCCGGCGATCAGCCGCATCAGGTCCTGGAAGTCCGATTGAAGCGTCTGAGCCGTCTGATATCGCGATCTCCAGCGGCGGGGGTTCCGCACCAGGTCCTCCAGGAAACTCCGCCGTCTCCGGTGGCGGACGCCGCGTTCGTTCTCCCTCTGCGCGATCCGCTGTTTCTCGAGGCCTACGTCCGGCAGTTGCTGTGCTCGCTGAATCTCCCCGCCGGCGCCTGCGGAGCCATCGGCCGGAGGATCGCGTCGTTCGTCGTCGAATTGGCGGGCGGAAACAGTGCGGCGAGCCTCCACATTGTGGCGTTGGTGAATTACATTGAGGAGTTTTGGATGGATCCGATCCCGGCGCCGGTGCCACCGAGCAGAGGGGCGGCGGAATCGGCGATCTCGAGGCTGAAGAAGGAGAAGGTTGAAGAAGGAGTAGAATTGGGAGATTGCAGCGTTTGTTTGAATGAGATGAATGGCGGGAGGCAGGTGATTAGGATTCCGTGCGGTCATGTGTATCATGAATCCTGCATCCTCACATGGCTGAGGAAGAGCAATTCTTGCCCATTGTGTAGAACAAAGTTGGAACAATGA

Coding sequence (CDS)

ATGCCTTTCGATACTTCCTCCACCGTCCGCTTCTGTCTCCACGGCGGCGCCGCCGCGGTTTCCTCCGGCGATCAGCCGCATCAGGTCCTGGAAGTCCGATTGAAGCGTCTGAGCCGTCTGATATCGCGATCTCCAGCGGCGGGGGTTCCGCACCAGGTCCTCCAGGAAACTCCGCCGTCTCCGGTGGCGGACGCCGCGTTCGTTCTCCCTCTGCGCGATCCGCTGTTTCTCGAGGCCTACGTCCGGCAGTTGCTGTGCTCGCTGAATCTCCCCGCCGGCGCCTGCGGAGCCATCGGCCGGAGGATCGCGTCGTTCGTCGTCGAATTGGCGGGCGGAAACAGTGCGGCGAGCCTCCACATTGTGGCGTTGGTGAATTACATTGAGGAGTTTTGGATGGATCCGATCCCGGCGCCGGTGCCACCGAGCAGAGGGGCGGCGGAATCGGCGATCTCGAGGCTGAAGAAGGAGAAGGTTGAAGAAGGAGTAGAATTGGGAGATTGCAGCGTTTGTTTGAATGAGATGAATGGCGGGAGGCAGGTGATTAGGATTCCGTGCGGTCATGTGTATCATGAATCCTGCATCCTCACATGGCTGAGGAAGAGCAATTCTTGCCCATTGTGTAGAACAAAGTTGGAACAATGA

Protein sequence

MPFDTSSTVRFCLHGGAAAVSSGDQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLRDPLFLEAYVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLEQ
Homology
BLAST of Moc01g15770 vs. NCBI nr
Match: XP_022151640.1 (E3 ubiquitin-protein ligase RNF181-like [Momordica charantia])

HSP 1 Score: 281.2 bits (718), Expect = 7.5e-72
Identity = 149/217 (68.66%), Postives = 172/217 (79.26%), Query Frame = 0

Query: 1   MPFDTSSTVRFCLHGGAAAVSSGDQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPS 60
           M   TS +VR+CLHG  + VSSGDQPHQVL+VRL+  SR+ISRSPAA     +LQET  +
Sbjct: 1   MSSSTSFSVRYCLHGHRSVVSSGDQPHQVLQVRLRSQSRMISRSPAA-----ILQETSWT 60

Query: 61  PVADAAFVLP---LRDPLFLEAYVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNS-AA 120
           P+A+A F LP   L   LFLEAY+RQLLCSL+LP GAC  I R+IASFVVELAGGNS   
Sbjct: 61  PMANAVFPLPLSALNGLLFLEAYIRQLLCSLDLPEGACEVIARKIASFVVELAGGNSDQV 120

Query: 121 SLHIVALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNG 180
           +LH+VALV+YIEE  +    AP     GAA+SAISRLKKEKVEE VELGDCSVCLNEMNG
Sbjct: 121 NLHVVALVDYIEEMAIVAEEAPA----GAAKSAISRLKKEKVEEEVELGDCSVCLNEMNG 180

Query: 181 GRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLEQ 214
           G +VI++PCGHVYHESCIL+WL K+NSCPLCRTKLEQ
Sbjct: 181 GSEVIKMPCGHVYHESCILSWLNKNNSCPLCRTKLEQ 208

BLAST of Moc01g15770 vs. NCBI nr
Match: XP_023539480.1 (E3 ubiquitin-protein ligase RNF165-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 197.2 bits (500), Expect = 1.4e-46
Identity = 105/211 (49.76%), Postives = 139/211 (65.88%), Query Frame = 0

Query: 24  DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLR---DPLFLEAY 83
           D P Q+L++R+   SRL++ SP +G P  ++ ET P+ +ADA F LPLR   DPLF +AY
Sbjct: 17  DGPDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLPTTMADAQFPLPLRELEDPLFCQAY 76

Query: 84  VRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVP 143
           +++ L S NL A    AI RRIASFVV++A GN   + +IVA ++YI+ FWMD       
Sbjct: 77  IKEFLSSYNLGATIKNAIARRIASFVVQMACGNYEMTFYIVAEIDYIQMFWMDDSATAAE 136

Query: 144 PS----------------RGAAESAISRLKKEKVE--EGVELGDCSVCLNEM-NGGRQVI 203
                             RGA E+AI RLKKEK +   G +LG+CSVC +EM +GG +V 
Sbjct: 137 EEEDEEEETEIFDDFSGRRGAPETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEVS 196

Query: 204 RIPCGHVYHESCILTWLRKSNSCPLCRTKLE 213
           RIPCGHVYH+SC+LTWL+++NSCPLCR KLE
Sbjct: 197 RIPCGHVYHKSCVLTWLQRNNSCPLCRNKLE 227

BLAST of Moc01g15770 vs. NCBI nr
Match: XP_023539471.1 (uncharacterized protein LOC111800118 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 191.0 bits (484), Expect = 1.0e-44
Identity = 103/223 (46.19%), Postives = 139/223 (62.33%), Query Frame = 0

Query: 24  DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLR---DPLFLEAY 83
           D P Q+L++R++  +RL++ SP +G P  ++ ET P+ +ADA F LPLR   DPLF +AY
Sbjct: 17  DDPDQLLQIRIRHSTRLLTGSPFSGYPFNIVHETLPTTMADAQFPLPLRELEDPLFCQAY 76

Query: 84  VRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVP 143
           + + L S NL A    AI RRIASFVV++A GN   + +IVA ++YI+ FW+D       
Sbjct: 77  IEEFLSSYNLGATIKNAIARRIASFVVQMACGNYEMTFYIVAEIDYIQMFWLDQSAIAAE 136

Query: 144 PS----------------------------RGAAESAISRLKKEKVE--EGVELGDCSVC 203
                                         RGA E+AI RLKKEK +   G +LG+CSVC
Sbjct: 137 EEEEEEEDEEEEEDEEEEEEEVIFDNFSGRRGAPETAIERLKKEKFDGFGGEDLGNCSVC 196

Query: 204 LNEM-NGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLE 213
            +EM +GG +V RIPCGHVYH+SC+LTWL+++NSCPLCR KLE
Sbjct: 197 YDEMKDGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRKKLE 239

BLAST of Moc01g15770 vs. NCBI nr
Match: KAG6596836.1 (hypothetical protein SDJN03_10016, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 177.9 bits (450), Expect = 9.0e-41
Identity = 99/222 (44.59%), Postives = 130/222 (58.56%), Query Frame = 0

Query: 24  DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLR---DPLFLEAY 83
           D P Q+L++R+    RL   SP  G P  ++ ET  +   DA F LPLR   DPLF  AY
Sbjct: 17  DGPDQLLQIRIGHSRRLFIGSPLPGYPFNIVYETLTTITVDAQFSLPLRELEDPLFCRAY 76

Query: 84  VRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVP 143
           + + L S NL A    AI +RIASFVV++A GN   + +IVA ++YI+ FW+D       
Sbjct: 77  IEEFLSSYNLDATINNAIAQRIASFVVQMACGNYEMTFYIVAEIDYIQMFWLDQSAIAAE 136

Query: 144 PS---------------------------RGAAESAISRLKKEKVE--EGVELGDCSVCL 203
                                        RGA E+AI RLKKEK +   G +LG+CSVC 
Sbjct: 137 EEEEEEEEEDEEEEEEEEEEVIFDNFSERRGAPETAIERLKKEKFDGFGGEDLGNCSVCY 196

Query: 204 NEM-NGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLE 213
           +EM  GG ++IRIPCGHVYH+SC+LTWL+++NSCPLCR K+E
Sbjct: 197 DEMKEGGEELIRIPCGHVYHKSCVLTWLQRNNSCPLCRKKIE 238

BLAST of Moc01g15770 vs. NCBI nr
Match: XP_023006825.1 (NEP1-interacting protein-like 1 [Cucurbita maxima])

HSP 1 Score: 173.3 bits (438), Expect = 2.2e-39
Identity = 99/222 (44.59%), Postives = 135/222 (60.81%), Query Frame = 0

Query: 1   MPFDTSSTVRFCLHGGAAAVSSGDQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPS 60
           MP   +ST+R+ L     A        Q L  RL   SRLISRSP+AG+P+ +L+ETP +
Sbjct: 1   MPLANASTLRYSLRRNHGA-------DQFLRFRLCYSSRLISRSPSAGLPYDILEETPST 60

Query: 61  PVADAAFVLPLR---DPLFLEAYVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAAS 120
           P+ADA F LPLR   DPLF  AY+ +L+ SLNL + +C  I ++I S V + A  +  A 
Sbjct: 61  PMADALFPLPLRELEDPLFCHAYLIELVSSLNLDSMSCNPIVQKITSLVTQWAAVDYDAK 120

Query: 121 LHIVALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKV----EEGVELGDCSVCLN- 180
             +VA ++YI+ FWM+  P  V   RG        LK+ +V    ++G+   +CSVC   
Sbjct: 121 FQLVADIDYIQMFWMEKRPGSV--GRGVMVEEKVVLKEAEVAAAEKQGIGGEECSVCYES 180

Query: 181 ---EMNGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKL 212
              E  G R+V +IPCGHV+H+SCILTW ++SNSCPLCR KL
Sbjct: 181 YEAEKGGEREVAKIPCGHVFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Moc01g15770 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.1e-12
Identity = 31/79 (39.24%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 133 DPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHES 192
           DP     PP+  +A  A+  +K  K     E+  C+VC++E   G  V ++PC HV+H+ 
Sbjct: 182 DPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQD 241

Query: 193 CILTWLRKSNSCPLCRTKL 212
           C+L WL   NSCP+CR +L
Sbjct: 242 CLLPWLELHNSCPVCRFEL 260

BLAST of Moc01g15770 vs. ExPASy Swiss-Prot
Match: Q9VE61 (E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN=CG7694 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.6e-11
Identity = 37/95 (38.95%), Postives = 54/95 (56.84%), Query Frame = 0

Query: 118 LHIVALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGG 177
           L ++A++N I+      I  P    R   E  +  + K   +EG +L +CSVC      G
Sbjct: 28  LQVLAIMNGIDM----EIEVPEASKRAILELPVHEIVKS--DEGGDL-ECSVCKEPAEEG 87

Query: 178 RQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLE 213
           ++   +PC H +HE CIL WL+K+NSCPLCR +LE
Sbjct: 88  QKYRILPCKHEFHEECILLWLKKTNSCPLCRYELE 115

BLAST of Moc01g15770 vs. ExPASy Swiss-Prot
Match: Q5M974 (E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 7.7e-11
Identity = 35/83 (42.17%), Postives = 45/83 (54.22%), Query Frame = 0

Query: 131 WMDPIPAPVPPSRGAAESAISRLKKEKV--EEGVELGDCSVCLNEMNGGRQVIRIPCGHV 190
           W   +P P      AA+  +  L K  V  E+      C VCL E   G  V ++PC H+
Sbjct: 47  WDQRLPPP------AAKKVVESLPKVTVTPEQADAALKCPVCLLEFEEGETVRQLPCEHL 106

Query: 191 YHESCILTWLRKSNSCPLCRTKL 212
           +H SCIL WL K+NSCPLCR +L
Sbjct: 107 FHSSCILPWLGKTNSCPLCRHEL 123

BLAST of Moc01g15770 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.0e-10
Identity = 34/79 (43.04%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 133 DPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHES 192
           DP     PP   A + A+  L   K+EE V    CSVCL+++  G Q  ++PC H +H S
Sbjct: 171 DPSRNGTPP---AKKEAVEALPTVKIEEVV---SCSVCLDDLEVGSQAKQMPCEHKFHSS 230

Query: 193 CILTWLRKSNSCPLCRTKL 212
           CIL WL   +SCP+CR +L
Sbjct: 231 CILPWLELHSSCPVCRFEL 243

BLAST of Moc01g15770 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.3e-10
Identity = 28/79 (35.44%), Postives = 44/79 (55.70%), Query Frame = 0

Query: 133 DPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHES 192
           DP     PP+  +A  A+  +   K     E   C+VC+++   G +  ++PC H+YH+ 
Sbjct: 190 DPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKD 249

Query: 193 CILTWLRKSNSCPLCRTKL 212
           C+L WL   NSCP+CR +L
Sbjct: 250 CLLPWLELHNSCPVCRHEL 268

BLAST of Moc01g15770 vs. ExPASy TrEMBL
Match: A0A6J1DBR5 (E3 ubiquitin-protein ligase RNF181-like OS=Momordica charantia OX=3673 GN=LOC111019547 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 3.6e-72
Identity = 149/217 (68.66%), Postives = 172/217 (79.26%), Query Frame = 0

Query: 1   MPFDTSSTVRFCLHGGAAAVSSGDQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPS 60
           M   TS +VR+CLHG  + VSSGDQPHQVL+VRL+  SR+ISRSPAA     +LQET  +
Sbjct: 1   MSSSTSFSVRYCLHGHRSVVSSGDQPHQVLQVRLRSQSRMISRSPAA-----ILQETSWT 60

Query: 61  PVADAAFVLP---LRDPLFLEAYVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNS-AA 120
           P+A+A F LP   L   LFLEAY+RQLLCSL+LP GAC  I R+IASFVVELAGGNS   
Sbjct: 61  PMANAVFPLPLSALNGLLFLEAYIRQLLCSLDLPEGACEVIARKIASFVVELAGGNSDQV 120

Query: 121 SLHIVALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNG 180
           +LH+VALV+YIEE  +    AP     GAA+SAISRLKKEKVEE VELGDCSVCLNEMNG
Sbjct: 121 NLHVVALVDYIEEMAIVAEEAPA----GAAKSAISRLKKEKVEEEVELGDCSVCLNEMNG 180

Query: 181 GRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLEQ 214
           G +VI++PCGHVYHESCIL+WL K+NSCPLCRTKLEQ
Sbjct: 181 GSEVIKMPCGHVYHESCILSWLNKNNSCPLCRTKLEQ 208

BLAST of Moc01g15770 vs. ExPASy TrEMBL
Match: A0A6J1KYV1 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499496 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 1.1e-39
Identity = 98/206 (47.57%), Postives = 122/206 (59.22%), Query Frame = 0

Query: 20  VSSGDQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLRD----PL 79
           V+SG QP Q+LE+RL   + LISRS       Q+LQ+TP +P+ DA F   LRD    P 
Sbjct: 23  VASGQQPIQLLELRLGHSTHLISRS-----SRQILQQTPSTPIGDALFPFVLRDLQHPPF 82

Query: 80  FLEAYVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPI 139
               YV Q L S ++P  AC  I   I+SF ++LA GN   + HIV  V+  E  W+D  
Sbjct: 83  LSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNMDLNFHIVVEVDVNEMIWVDLD 142

Query: 140 PAPV-----PPSRGAAESAISRLKKEK---VEEGVELGDCSVCLNEMNGGRQVIRIPCGH 199
           P  V         GA  SAI RL+++K   + E  E GDCSVC   + G  +V RIPCGH
Sbjct: 143 PFLVEEYSPAEMNGAPASAIERLQRQKFDGLREEEEEGDCSVCCEALKGEEEVSRIPCGH 202

Query: 200 VYHESCILTWLRKSNSCPLCRTKLEQ 214
           VYH+ CIL WL+ SNSCPLCRTKLEQ
Sbjct: 203 VYHKFCILKWLQMSNSCPLCRTKLEQ 223

BLAST of Moc01g15770 vs. ExPASy TrEMBL
Match: A0A6J1L186 (NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 1.1e-39
Identity = 99/222 (44.59%), Postives = 135/222 (60.81%), Query Frame = 0

Query: 1   MPFDTSSTVRFCLHGGAAAVSSGDQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPS 60
           MP   +ST+R+ L     A        Q L  RL   SRLISRSP+AG+P+ +L+ETP +
Sbjct: 1   MPLANASTLRYSLRRNHGA-------DQFLRFRLCYSSRLISRSPSAGLPYDILEETPST 60

Query: 61  PVADAAFVLPLR---DPLFLEAYVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAAS 120
           P+ADA F LPLR   DPLF  AY+ +L+ SLNL + +C  I ++I S V + A  +  A 
Sbjct: 61  PMADALFPLPLRELEDPLFCHAYLIELVSSLNLDSMSCNPIVQKITSLVTQWAAVDYDAK 120

Query: 121 LHIVALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKV----EEGVELGDCSVCLN- 180
             +VA ++YI+ FWM+  P  V   RG        LK+ +V    ++G+   +CSVC   
Sbjct: 121 FQLVADIDYIQMFWMEKRPGSV--GRGVMVEEKVVLKEAEVAAAEKQGIGGEECSVCYES 180

Query: 181 ---EMNGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKL 212
              E  G R+V +IPCGHV+H+SCILTW ++SNSCPLCR KL
Sbjct: 181 YEAEKGGEREVAKIPCGHVFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Moc01g15770 vs. ExPASy TrEMBL
Match: A0A6J1G7Z5 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC111451666 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 4.1e-39
Identity = 97/206 (47.09%), Postives = 122/206 (59.22%), Query Frame = 0

Query: 20  VSSGDQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLRD----PL 79
           V+S  QP Q+LE+RL   + LISRS       Q+LQ+TP +P+ DA F   LRD    P 
Sbjct: 23  VASDQQPMQLLELRLGNSTHLISRS-----SRQILQQTPTTPMGDALFPFVLRDLQHPPF 82

Query: 80  FLEAYVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPI 139
               YV Q L S ++P  AC  I   I+SF ++LA GN   + HIV  V+ IE  W+D  
Sbjct: 83  LSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNFHIVVEVDVIEMIWVDLD 142

Query: 140 PAPV-----PPSRGAAESAISRLKKEK---VEEGVELGDCSVCLNEMNGGRQVIRIPCGH 199
           P  V         GA  SAI RL++++   + E  E GDCSVC   + G  +V RIPCGH
Sbjct: 143 PFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVCCEALKGEEEVSRIPCGH 202

Query: 200 VYHESCILTWLRKSNSCPLCRTKLEQ 214
           VYH+ CIL WL+ SNSCPLCRTKLEQ
Sbjct: 203 VYHKCCILKWLQMSNSCPLCRTKLEQ 223

BLAST of Moc01g15770 vs. ExPASy TrEMBL
Match: A0A6J1G7L1 (NEP1-interacting protein-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111451601 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 2.9e-37
Identity = 95/222 (42.79%), Postives = 134/222 (60.36%), Query Frame = 0

Query: 1   MPFDTSSTVRFCLHGGAAAVSSGDQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPS 60
           MPF  +ST+R+ L     A        Q+L++RL   SRLISRSP+AG+P+ +L+ETP +
Sbjct: 1   MPFANASTLRYSLRRNHGA-------DQLLQLRLGYSSRLISRSPSAGLPYDILEETPST 60

Query: 61  PVADAAFVLPLR---DPLFLEAYVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAAS 120
           P+ADA F LPLR   DPLF  AY+ +L+ S NL +     I ++I SFV + A  +  A 
Sbjct: 61  PMADALFPLPLRELEDPLFCHAYLVELVSSFNLGSVTWNPIVQKITSFVTQWAAVDYDAK 120

Query: 121 LHIVALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKV----EEGVELGDCSVCLN- 180
             +VA ++YI+ FWM+     V   RG        L++  V    ++G+   +CSVC   
Sbjct: 121 FQLVADIDYIQMFWMEKHRGSV--GRGVMVEERVVLEETGVAAAEKQGIGGEECSVCYES 180

Query: 181 ---EMNGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKL 212
              E  G ++V +IPCGH +H+SCILTW ++SNSCPLCR KL
Sbjct: 181 YEAEKGGEKEVAKIPCGHAFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Moc01g15770 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 6.5e-13
Identity = 31/79 (39.24%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 133 DPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHES 192
           DP     PP+  +A  A+  +K  K     E+  C+VC++E   G  V ++PC HV+H+ 
Sbjct: 182 DPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQD 241

Query: 193 CILTWLRKSNSCPLCRTKL 212
           C+L WL   NSCP+CR +L
Sbjct: 242 CLLPWLELHNSCPVCRFEL 260

BLAST of Moc01g15770 vs. TAIR 10
Match: AT5G54990.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 8.4e-13
Identity = 24/45 (53.33%), Postives = 35/45 (77.78%), Query Frame = 0

Query: 166 DCSVCLNEMNGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTK 211
           DC +CL E++ G   +++PC HV+H  CI+TWL+K+ SCP+CRTK
Sbjct: 173 DCPICLTELSSGVSRMKLPCSHVFHRDCIMTWLKKNPSCPICRTK 217

BLAST of Moc01g15770 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-12
Identity = 34/93 (36.56%), Postives = 49/93 (52.69%), Query Frame = 0

Query: 122 ALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKVEE---GVELGDCSVCLNEMNGGR 181
           +L   IE+   D  P P P    A+E  I+ L   K+       ++  C+VC+ E   G 
Sbjct: 180 SLEQLIEQLTQDDRPGPPP----ASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGG 239

Query: 182 QVIRIPCGHVYHESCILTWLRKSNSCPLCRTKL 212
               +PC H+YH+ CI+ WLR +NSCP+CR  L
Sbjct: 240 DATELPCKHIYHKDCIVPWLRLNNSCPICRRDL 268

BLAST of Moc01g15770 vs. TAIR 10
Match: AT4G05350.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.4e-12
Identity = 51/199 (25.63%), Postives = 93/199 (46.73%), Query Frame = 0

Query: 22  SGDQPHQVLEVRLKRLS-RLISRSPAAGVPHQVLQETPPSPVADAAFVLPLRDPLFLEAY 81
           S  +P+ V+ +RLK  S  +I  +   G  ++  Q +PP       F++ +        +
Sbjct: 20  SPSEPNSVI-IRLKTKSVEIIIENQTTGRQNRRTQVSPP-------FLIDINLRSSSHDH 79

Query: 82  VRQL----LCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIP 141
           +R L    L      +  C  +  +I++  ++L  G++  +L + A V Y        + 
Sbjct: 80  IRTLLHDRLVGYPFESYLCDDLAPKISTAAIDLGFGHNGFTLTMAATVTY------RTVS 139

Query: 142 APVPPSRGAAESAISRLKKEKVEE-GVELGDCSVCLNEMNGG---RQVIRIPCGHVYHES 201
                 +      + R+K E+ +   +E   CS+CL  +  G   R V R+ C HV+H  
Sbjct: 140 VMSNDEKSLRMVLLGRMKAEEFKSLKMETEPCSICLESLVSGPKPRDVTRMTCSHVFHNG 199

Query: 202 CILTWLRKSNSCPLCRTKL 212
           C+L WL++ N+CPLCRT++
Sbjct: 200 CLLEWLKRKNTCPLCRTEI 204

BLAST of Moc01g15770 vs. TAIR 10
Match: AT1G18770.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-11
Identity = 28/74 (37.84%), Postives = 43/74 (58.11%), Query Frame = 0

Query: 138 PVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHESCILTW 197
           P P S+   +S   ++ K       E+  C +CL E + GR+V+ +PCGH + + C+L W
Sbjct: 32  PTPASKLVVKSLARKIYKMTTSSTGEM--CIICLEEFSEGRRVVTLPCGHDFDDECVLKW 91

Query: 198 LRKSNSCPLCRTKL 212
              ++SCPLCR KL
Sbjct: 92  FETNHSCPLCRFKL 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151640.17.5e-7268.66E3 ubiquitin-protein ligase RNF181-like [Momordica charantia][more]
XP_023539480.11.4e-4649.76E3 ubiquitin-protein ligase RNF165-like [Cucurbita pepo subsp. pepo][more]
XP_023539471.11.0e-4446.19uncharacterized protein LOC111800118 [Cucurbita pepo subsp. pepo][more]
KAG6596836.19.0e-4144.59hypothetical protein SDJN03_10016, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023006825.12.2e-3944.59NEP1-interacting protein-like 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8LPN79.1e-1239.24E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q9VE612.6e-1138.95E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN... [more]
Q5M9747.7e-1142.17E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 ... [more]
Q6AVN21.0e-1043.04E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
P0CH301.3e-1035.44E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1DBR53.6e-7268.66E3 ubiquitin-protein ligase RNF181-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1KYV11.1e-3947.57E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499... [more]
A0A6J1L1861.1e-3944.59NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4... [more]
A0A6J1G7Z54.1e-3947.09E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1G7L12.9e-3742.79NEP1-interacting protein-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111451601 PE... [more]
Match NameE-valueIdentityDescription
AT3G19950.16.5e-1339.24RING/U-box superfamily protein [more]
AT5G54990.18.4e-1353.33RING/U-box superfamily protein [more]
AT1G60360.11.1e-1236.56RING/U-box superfamily protein [more]
AT4G05350.11.4e-1225.63RING/U-box superfamily protein [more]
AT1G18770.11.2e-1137.84RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 167..207
e-value: 1.0E-8
score: 44.9
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 166..208
e-value: 2.6E-15
score: 56.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 167..208
score: 13.374458
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 139..213
e-value: 7.6E-21
score: 75.6
NoneNo IPR availablePANTHERPTHR45931SI:CH211-59O9.10coord: 81..212
NoneNo IPR availablePANTHERPTHR45931:SF9E3 UBIQUITIN-PROTEIN LIGASE RNF12-B-LIKEcoord: 81..212
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 166..211

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g15770.1Moc01g15770.1mRNA