Homology
BLAST of MS028268 vs. NCBI nr
Match:
XP_022135680.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])
HSP 1 Score: 704.1 bits (1816), Expect = 6.0e-199
Identity = 348/353 (98.58%), Postives = 353/353 (100.00%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
MEMTRRESISKKEE+EEEHARV+IWKYIFGFVEMAVVKCAIELKIA+AIETQTSPMTLSQ
Sbjct: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP 120
LSSAL+CSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP
Sbjct: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP 120
Query: 121 TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMITVPAI 180
TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALIN+AMACSGRMITVPAI
Sbjct: 121 TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAI 180
Query: 181 LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG 240
LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG
Sbjct: 181 LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG 240
Query: 241 DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS 300
DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS
Sbjct: 241 DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS 300
Query: 301 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL 354
DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL
Sbjct: 301 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL 353
BLAST of MS028268 vs. NCBI nr
Match:
XP_022135713.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])
HSP 1 Score: 533.5 bits (1373), Expect = 1.4e-147
Identity = 259/357 (72.55%), Postives = 306/357 (85.71%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
M++ +ES SKKEE + EHARV++W YIFGF E+A++KCAIELKIAD IE+ SPMTLS+
Sbjct: 11 MDLALKESTSKKEE-DHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSK 70
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSSALNCSPPLL+RILRFL RGIF+E+TT +NLTGY+ TPLSRLL + SMAPFLLL
Sbjct: 71 LSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLL 130
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMIT 180
ES+P MLAPW LS +K +G PFEA HG D+WSY A D H+ + N+AMAC+ R+IT
Sbjct: 131 ESNPVMLAPWHNLSALIKAKDG-TPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVIT 190
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILEGC EIFEGVG +VDVGGGNGT+LSMIVKA PWI+GINFDLPHV+SVAQEC+GVQ
Sbjct: 191 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQ 250
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G+MFD IPKADAA +MWVLHDW+DE+CIKILKNC+DAIP+ GKVIIV+ VI+ EE
Sbjct: 251 HVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEE 310
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL 354
NNL DVGL+LDMVMMAHTNKGKERT +EWAYVLH+AGF+RYTITP++AVQS+IQ F+
Sbjct: 311 NNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365
BLAST of MS028268 vs. NCBI nr
Match:
XP_022136086.1 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica charantia])
HSP 1 Score: 530.8 bits (1366), Expect = 9.0e-147
Identity = 262/357 (73.39%), Postives = 303/357 (84.87%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
M++ +ES SKKEE + E A V++W YIFGF +MA++KCAIELKIAD IE+ SPMTLSQ
Sbjct: 1 MDLALKESTSKKEE-DHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQ 60
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSSALNCSPPLL+RILRFL RGIF+E+TT +NLTGY+ TPLSRLL + SMAP LLL
Sbjct: 61 LSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMIT 180
ESSP MLA W LS +K +G PFEA HG D+WSY A D H+ + N+AMAC+ R+IT
Sbjct: 121 ESSPIMLASWHNLSARIKASDG-TPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVIT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILEGC EIFEGVG +VDVGGGNGT+LSMIVKA PWIRGINFDLPHV+SVAQECMGVQ
Sbjct: 181 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G+MFD IPKADAA LM VLHDW+DE+CIKILKNCKDA+P+ TGKVIIV+TVI+ EE
Sbjct: 241 HVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL 354
NLSDVGLMLDMVMMA TNKGKERTC+EWA+VLH++GFSRYTITPIRA+QS+IQ F+
Sbjct: 301 KNLSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355
BLAST of MS028268 vs. NCBI nr
Match:
XP_038888665.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])
HSP 1 Score: 523.5 bits (1347), Expect = 1.4e-144
Identity = 264/357 (73.95%), Postives = 295/357 (82.63%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
M+ +ESI KKEE E+E ARV+IW YIFGFVE+A+VKCAIELKIAD IET S MTLSQ
Sbjct: 1 MDFAFKESIPKKEE-EDEQARVQIWNYIFGFVEIAIVKCAIELKIADTIETHGSSMTLSQ 60
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LS ALNCS LL+RILRFLV RGIF+++ T ENLT YS TPLSRLL + SM P +LL
Sbjct: 61 LSLALNCSSSLLYRILRFLVNRGIFKQEITEENLTSYSQTPLSRLLASSNNNSMVPLVLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMIT 180
ESSP MLAPW +LS +K G PFEAAHG D+WS+AA D +H+ +INEAM+C+ R++T
Sbjct: 121 ESSPVMLAPWHRLSDRIKANEG-TPFEAAHGKDLWSFAAADPIHNTIINEAMSCTARVLT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILE C EIFEG+G +VDVGGGNGT LSMIVKA PWI+GIN DLPHVIS +QE GVQ
Sbjct: 181 VPAILEDCPEIFEGIGSLVDVGGGNGTCLSMIVKACPWIKGINLDLPHVISSSQEYTGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G M DSIPKADAA MWVLHDWDDE CIKILKNC+DAIP TGKVIIV+ VI EKEE
Sbjct: 241 HVSGSMLDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL 354
NNLSDVGLMLDMVMMAHTN GKERT KEW YVLHQAGF+RYTITPIRAVQS+IQGFL
Sbjct: 301 NNLSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITPIRAVQSVIQGFL 355
BLAST of MS028268 vs. NCBI nr
Match:
XP_023554119.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 520.4 bits (1339), Expect = 1.2e-143
Identity = 256/356 (71.91%), Postives = 300/356 (84.27%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
M+ ++SISKKE E EHAR+++W+YIFGFVEMA++KCAIELKI D I++ MTL +
Sbjct: 1 MDFALKQSISKKE--EHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPE 60
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSS+LNCS LLHRI+RFLV RGIF+++ T ENLT YSHTPLSRLLT+ SMAPF+LL
Sbjct: 61 LSSSLNCSSSLLHRIMRFLVHRGIFKQEITGENLTRYSHTPLSRLLTSGSENSMAPFVLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMIT 180
ES+P MLAPW LS +K GN PFEAAHG D+WSYAA D +H+A+ N+AMACS R+++
Sbjct: 121 ESNPVMLAPWHSLSARIK-GNEAIPFEAAHGKDIWSYAAADPIHNAMFNDAMACSARVMS 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILE C EIFEGV C+VDVGGGNGT+LSMIVKA PWI+GINFDLPHVI+ +GVQ
Sbjct: 181 VPAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVIASLPADIGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HVGG+MFD IPKADAA LMWVLHDWDDE CIKILKNC+DAIP+ TGKVIIV+ V+ K+E
Sbjct: 241 HVGGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGF 353
NNLSDVGLMLDMVMMAHTN GKERT KEWAYVLHQAGF+R+TITPIRA+QS+IQ F
Sbjct: 301 NNLSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 353
BLAST of MS028268 vs. ExPASy Swiss-Prot
Match:
Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)
HSP 1 Score: 363.2 bits (931), Expect = 3.3e-99
Identity = 192/369 (52.03%), Postives = 252/369 (68.29%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTS--PMTL 60
++ + E +K EE A ++IWKY+FGF ++A KCAI+LKI +AIE S P+TL
Sbjct: 10 LDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTL 69
Query: 61 SQLSSALNCSPPLLHRILRFLVRRGIFREQTTTENL-TGYSHTPLSRLLT------TSMA 120
++LSSA++ SP L RI+RFLV +GIF+E T + L TGY +TPLSR L S+A
Sbjct: 70 AELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLA 129
Query: 121 PFLLLESSPTMLAPWQKLSTCVK---EGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAM 180
PF+L E++P MLAPW +LS+ V G+ PF+A HG DVWS+A + S +INEAM
Sbjct: 130 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 189
Query: 181 ACSGRMITVPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISV 240
AC R + VP + C +F+GV +VDVGGG G + M+VK +PWI+G NFDLPHVI V
Sbjct: 190 ACDARRV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEV 249
Query: 241 AQECMGVQHVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVD 300
A+ GV++V GDMFDSIP DA + WVLHDW D++CIKILKNCK+A+P GKV+IV+
Sbjct: 250 AEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVE 309
Query: 301 TVINE--------KEENNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTIT 350
+VI E + + L V LMLDMVMMAHT+ GKERT KEW +VL +AGF+RY +
Sbjct: 310 SVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVR 369
BLAST of MS028268 vs. ExPASy Swiss-Prot
Match:
Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)
HSP 1 Score: 270.0 bits (689), Expect = 3.8e-71
Identity = 151/352 (42.90%), Postives = 213/352 (60.51%), Query Frame = 0
Query: 15 QEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQLSSALNCSPP---- 74
+E + EIW+++F FV+ +KCA+EL I D I + P+T+S++ +L + P
Sbjct: 5 EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64
Query: 75 ---LLHRILRFLVRRGIFREQTTTE-NLTGYSHTPLSRLLTT----SMAPFLLLESSPTM 134
L RI+R LV + +F + E N Y+ T S+ L +++P +L E++P +
Sbjct: 65 NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124
Query: 135 LAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMITVPAILE 194
L PWQ L C +E + +PFE AHG ++W A D + +N AM CS I +LE
Sbjct: 125 LKPWQYLGKCAQEKS--SPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLE 184
Query: 195 GCKEIFEGV-GCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGD 254
K+ F G+ G +VDVGGG G+ ++ IVKA P I+GINFDLPHV++ A E GV+HVGGD
Sbjct: 185 -YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGD 244
Query: 255 MFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAI-PRTTGKVIIVDTVINEKEENNLS 314
MF IP+ADA ++ W+LHDW DE+C ILKNC AI + GKVIIVD V+ +
Sbjct: 245 MFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFD 304
Query: 315 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGF 353
+GL+ D++MMAHT GKERT EW +L+ AGF RY + A II+ F
Sbjct: 305 KMGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAF 353
BLAST of MS028268 vs. ExPASy Swiss-Prot
Match:
B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)
HSP 1 Score: 262.7 bits (670), Expect = 6.0e-69
Identity = 156/361 (43.21%), Postives = 224/361 (62.05%), Query Frame = 0
Query: 12 KEEQEEEHARVE--IWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQLSSALNCSP 71
+ +Q E R E +WK I G + V+KCA+EL+I D + + ++P+TL+Q++S++ SP
Sbjct: 5 RNDQTEAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSVPDSP 64
Query: 72 PL----LHRILRFLVRRGIFREQTT---TENLTGYSHTPLSRLLTT--------SMAPFL 131
L L RI+R LVRR IF + + E L G +H+ SRLL + ++APF+
Sbjct: 65 SLNLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHS--SRLLLSKTTLPDQVTLAPFV 124
Query: 132 LLESSPTMLAPWQKLSTCVKE-GNGIAPFEAAH-GMDVWSYAAGDAVHSALINEAMACSG 191
+ P + APW L+ CVKE GNG FE H G +W + G+ + + N+ MA +
Sbjct: 125 AFMTHPYLSAPWSCLARCVKEGGNG---FEMVHGGRQLWDLSPGNPEFNKVFNDGMASTA 184
Query: 192 RMITVPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQEC 251
R IT AIL +++F G+ +VDVGG G ++S IVK+ P I+GIN+DLPHV++ A
Sbjct: 185 R-ITTMAILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPTY 244
Query: 252 MG-VQHVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVI 311
G V HVGG+MF+ IP A A + W+LHDW DE+C+KILKNC+ A+P GK+IIVD V+
Sbjct: 245 TGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIVL 304
Query: 312 NEKEENNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG 353
+ D +MLD+ +MA T +GKERT KEW VL + GF RY I I A+ S+I+
Sbjct: 305 EPEGNGLFDDAAVMLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEA 358
BLAST of MS028268 vs. ExPASy Swiss-Prot
Match:
Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)
HSP 1 Score: 258.1 bits (658), Expect = 1.5e-67
Identity = 135/340 (39.71%), Postives = 207/340 (60.88%), Query Frame = 0
Query: 21 RVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQLSSAL---NCSPPLLHRILR 80
+ ++W I+GF + V++CA+E+ IAD I+ +TL+QL++ L N S L+R++R
Sbjct: 20 QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVR 79
Query: 81 FLVRRGIFREQTTTENLTG-YSHTPLSRLL----TTSMAPFLLLESSPTMLAPWQKLSTC 140
+LV I ++T + YS P+ LL SM P +L + + W +
Sbjct: 80 YLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEG 139
Query: 141 VKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMITVPAILEGCKEIFEGVG 200
+ G+ A FE GMD+W Y G+ S L NE MA R++T ++E C++ F+G+
Sbjct: 140 LGNGSTTA-FEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT-KTLIEDCRDTFQGLD 199
Query: 201 CVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFDSIPKADAA 260
+VD+GGGNGT + I +A+P I+ +DLPHV++ + + ++ V GDMF S+P A A
Sbjct: 200 SLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAI 259
Query: 261 LLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLSDVGLMLDMVMMA 320
LL +LHDW DEEC+ ILK CK+AIP+ TGKVIIVD + E+ + L+ L+LD+ M+
Sbjct: 260 LLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLV 319
Query: 321 HTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGF 353
+T G+ERT +W +L +AGF + I PIRA+QS+I+ F
Sbjct: 320 NTG-GRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAF 356
BLAST of MS028268 vs. ExPASy Swiss-Prot
Match:
Q9LEL5 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)
HSP 1 Score: 256.1 bits (653), Expect = 5.6e-67
Identity = 140/350 (40.00%), Postives = 214/350 (61.14%), Query Frame = 0
Query: 12 KEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQLSSAL-----N 71
K++ + A+ +WK I+GF + V++CA+EL I D I+ PM L+ L+S L N
Sbjct: 6 KDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVN 65
Query: 72 CSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLT----TSMAPFLLLESSPTM 131
C L+RILR+LV+ I R + + + Y+ P++ LL+ SM P +L +
Sbjct: 66 CDN--LYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDF 125
Query: 132 LAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMITVPAILE 191
+ PW + + + NG A FE A GM +W Y G S L NE MA R++T +++
Sbjct: 126 MTPWHSMKDGLSD-NGTA-FEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLT-SSLIS 185
Query: 192 GCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDM 251
G +++F+G+ +VDVGGGNGT + I A+P I+ FDLPHVI+ + + ++ +GGDM
Sbjct: 186 GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDM 245
Query: 252 FDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLSDV 311
F S+P A A +L +LHDW+DE+ IKILK C++A+P+ GKVIIVD ++E+ ++ LS
Sbjct: 246 FKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 305
Query: 312 GLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGF 353
L+LD+ M+ +T GKERT + W ++ AGFS I I A+QS+I+ F
Sbjct: 306 RLILDIDMLVNTG-GKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVF 349
BLAST of MS028268 vs. ExPASy TrEMBL
Match:
A0A6J1C1Q0 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007578 PE=3 SV=1)
HSP 1 Score: 704.1 bits (1816), Expect = 2.9e-199
Identity = 348/353 (98.58%), Postives = 353/353 (100.00%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
MEMTRRESISKKEE+EEEHARV+IWKYIFGFVEMAVVKCAIELKIA+AIETQTSPMTLSQ
Sbjct: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP 120
LSSAL+CSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP
Sbjct: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP 120
Query: 121 TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMITVPAI 180
TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALIN+AMACSGRMITVPAI
Sbjct: 121 TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAI 180
Query: 181 LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG 240
LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG
Sbjct: 181 LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG 240
Query: 241 DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS 300
DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS
Sbjct: 241 DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS 300
Query: 301 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL 354
DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL
Sbjct: 301 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL 353
BLAST of MS028268 vs. ExPASy TrEMBL
Match:
A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)
HSP 1 Score: 533.5 bits (1373), Expect = 6.7e-148
Identity = 259/357 (72.55%), Postives = 306/357 (85.71%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
M++ +ES SKKEE + EHARV++W YIFGF E+A++KCAIELKIAD IE+ SPMTLS+
Sbjct: 11 MDLALKESTSKKEE-DHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSK 70
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSSALNCSPPLL+RILRFL RGIF+E+TT +NLTGY+ TPLSRLL + SMAPFLLL
Sbjct: 71 LSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLL 130
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMIT 180
ES+P MLAPW LS +K +G PFEA HG D+WSY A D H+ + N+AMAC+ R+IT
Sbjct: 131 ESNPVMLAPWHNLSALIKAKDG-TPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVIT 190
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILEGC EIFEGVG +VDVGGGNGT+LSMIVKA PWI+GINFDLPHV+SVAQEC+GVQ
Sbjct: 191 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQ 250
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G+MFD IPKADAA +MWVLHDW+DE+CIKILKNC+DAIP+ GKVIIV+ VI+ EE
Sbjct: 251 HVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEE 310
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL 354
NNL DVGL+LDMVMMAHTNKGKERT +EWAYVLH+AGF+RYTITP++AVQS+IQ F+
Sbjct: 311 NNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365
BLAST of MS028268 vs. ExPASy TrEMBL
Match:
A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)
HSP 1 Score: 530.8 bits (1366), Expect = 4.4e-147
Identity = 262/357 (73.39%), Postives = 303/357 (84.87%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
M++ +ES SKKEE + E A V++W YIFGF +MA++KCAIELKIAD IE+ SPMTLSQ
Sbjct: 1 MDLALKESTSKKEE-DHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQ 60
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSSALNCSPPLL+RILRFL RGIF+E+TT +NLTGY+ TPLSRLL + SMAP LLL
Sbjct: 61 LSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMIT 180
ESSP MLA W LS +K +G PFEA HG D+WSY A D H+ + N+AMAC+ R+IT
Sbjct: 121 ESSPIMLASWHNLSARIKASDG-TPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVIT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILEGC EIFEGVG +VDVGGGNGT+LSMIVKA PWIRGINFDLPHV+SVAQECMGVQ
Sbjct: 181 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G+MFD IPKADAA LM VLHDW+DE+CIKILKNCKDA+P+ TGKVIIV+TVI+ EE
Sbjct: 241 HVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGFL 354
NLSDVGLMLDMVMMA TNKGKERTC+EWA+VLH++GFSRYTITPIRA+QS+IQ F+
Sbjct: 301 KNLSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355
BLAST of MS028268 vs. ExPASy TrEMBL
Match:
A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)
HSP 1 Score: 508.4 bits (1308), Expect = 2.3e-140
Identity = 252/356 (70.79%), Postives = 297/356 (83.43%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
M+ ++SISKKE E EHAR+++W+YIFGFVEMA++KCAIELKI D I++ MTL +
Sbjct: 1 MDFALKQSISKKE--EHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPE 60
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLT----TSMAPFLLL 120
LSS+LNCS LL+RI+RFLV RGIF+E+ T ENLT YSHTPLSRLL +SMAP LLL
Sbjct: 61 LSSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMIT 180
ES+P MLAPW LS +K GN PFEAAHG D+WSYAA + H+A+ N+AMACS R++T
Sbjct: 121 ESNPVMLAPWHGLSARIK-GNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILE C EIFEGV C+VDVGGGNGT+LSMIVKA PWI+GINFDLPHV++ + +GVQ
Sbjct: 181 VPAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HVGG+MFD IPKADAA LMWVLH WDDE CIKILK C++AIP+ TGKVIIV+ V+ EKEE
Sbjct: 241 HVGGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQGF 353
NNLSDVGLMLDMVMMAHTN GKERT +EWAYVLHQAGF+R+TITPIRA+QS+IQ F
Sbjct: 301 NNLSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353
BLAST of MS028268 vs. ExPASy TrEMBL
Match:
A0A5D3DJ08 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004240 PE=3 SV=1)
HSP 1 Score: 505.0 bits (1299), Expect = 2.6e-139
Identity = 255/361 (70.64%), Postives = 295/361 (81.72%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTSPMTLSQ 60
M + SISKKEE+E ARV+IWKYIFGFVEMA+VKCAIEL+I D IE+ SPMTLSQ
Sbjct: 1 MNFAPQVSISKKEEEE---ARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQ 60
Query: 61 LSSALNCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LS+ALNCS LL+RILRFLVRRGIF+++ T EN+ Y HTPLSRLL + SMAP LLL
Sbjct: 61 LSTALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMIT 180
ESSP MLAPW +LS +K GNG PFEAAHG DVWS+AA D +H+ +IN+AM+C+ R+ T
Sbjct: 121 ESSPVMLAPWHRLSARIK-GNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILE C +IFEG+G +VDVGGGNGT LSMIVKA+PWI+GINFDLPHVIS +Q+ +GV+
Sbjct: 181 VPAILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVE 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HVGG+M DSIPKADAA +MWVLHDWDDE CIKILKNCK+AI GKVIIV+ +I E+ E
Sbjct: 241 HVGGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKEAISEKRGKVIIVEAIIEERSE 300
Query: 301 -----NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG 353
NNL DVGLMLDMVMMAHT GKERT KEW VLHQAGF+RYTITPIRAV S+IQG
Sbjct: 301 EENNNNNLGDVGLMLDMVMMAHTENGKERTIKEWGNVLHQAGFTRYTITPIRAVHSVIQG 357
BLAST of MS028268 vs. TAIR 10
Match:
AT4G35160.1 (O-methyltransferase family protein )
HSP 1 Score: 363.2 bits (931), Expect = 2.3e-100
Identity = 192/369 (52.03%), Postives = 252/369 (68.29%), Query Frame = 0
Query: 1 MEMTRRESISKKEEQEEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTS--PMTL 60
++ + E +K EE A ++IWKY+FGF ++A KCAI+LKI +AIE S P+TL
Sbjct: 10 LDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTL 69
Query: 61 SQLSSALNCSPPLLHRILRFLVRRGIFREQTTTENL-TGYSHTPLSRLLT------TSMA 120
++LSSA++ SP L RI+RFLV +GIF+E T + L TGY +TPLSR L S+A
Sbjct: 70 AELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLA 129
Query: 121 PFLLLESSPTMLAPWQKLSTCVK---EGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAM 180
PF+L E++P MLAPW +LS+ V G+ PF+A HG DVWS+A + S +INEAM
Sbjct: 130 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 189
Query: 181 ACSGRMITVPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISV 240
AC R + VP + C +F+GV +VDVGGG G + M+VK +PWI+G NFDLPHVI V
Sbjct: 190 ACDARRV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEV 249
Query: 241 AQECMGVQHVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVD 300
A+ GV++V GDMFDSIP DA + WVLHDW D++CIKILKNCK+A+P GKV+IV+
Sbjct: 250 AEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVE 309
Query: 301 TVINE--------KEENNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTIT 350
+VI E + + L V LMLDMVMMAHT+ GKERT KEW +VL +AGF+RY +
Sbjct: 310 SVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVR 369
BLAST of MS028268 vs. TAIR 10
Match:
AT4G35150.1 (O-methyltransferase family protein )
HSP 1 Score: 316.6 bits (810), Expect = 2.5e-86
Identity = 167/345 (48.41%), Postives = 222/345 (64.35%), Query Frame = 0
Query: 16 EEEHARVEIWKYIFGFVEMAVVKCAIELKIADAIETQTS--PMTLSQLSSALNCSPPLLH 75
EE A ++IW+Y+FGF ++A KCAI+LKI +AIE S P+TLS+LSSA++ SP L
Sbjct: 11 EEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHLR 70
Query: 76 RILRFLVRRGIFREQTTTENL-TGYSHTPLSRLLTTSMAPFLLLESSPTMLAPWQKLSTC 135
RI+RFLV +G+F+E T + L TGY++TPLSR + +
Sbjct: 71 RIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMMIT----------------------- 130
Query: 136 VKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMITVPAILEGCKEIFEGVG 195
HG D+W++A + HS LINEAMAC R + VP + C+ +F+GV
Sbjct: 131 -----------KLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPRVAGACQGLFDGVA 190
Query: 196 CVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFDSIPKADAA 255
VVDVGGG G + ++VK +PWI+G NFDLPHVI VAQ GV++V GDMFDSIP +DA
Sbjct: 191 TVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPASDAV 250
Query: 256 LLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKE--------ENNLSDVGL 315
++ WVLHDW D++CIKILKNCK+A+ GKV+IV+ VI EK+ ++ L V L
Sbjct: 251 IIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKLEHVRL 310
Query: 316 MLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSII 350
LDMVMM HT+ GKERT KEW +VL +AGF+RY + VQS+I
Sbjct: 311 QLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320
BLAST of MS028268 vs. TAIR 10
Match:
AT5G54160.1 (O-methyltransferase 1 )
HSP 1 Score: 189.9 bits (481), Expect = 3.5e-48
Identity = 115/314 (36.62%), Postives = 174/314 (55.41%), Query Frame = 0
Query: 37 VKCAIELKIADAIETQTSPMTLSQLSSALNC----SPPLLHRILRFLVRRGIFREQTTTE 96
+K A+EL + + + SPM+ ++++S L +P +L RILR L + +
Sbjct: 36 LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVL--TCSNR 95
Query: 97 NLTG------YSHTPLSRLLT-----TSMAPFLLLESSPTMLAPWQKLSTCVKEGNGIAP 156
L+G Y P+ + LT S+A L+ ++ W L + +G P
Sbjct: 96 KLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGG--IP 155
Query: 157 FEAAHGMDVWSYAAGDAVHSALINEAMACSGRMITVPAILEGCKEIFEGVGCVVDVGGGN 216
F A+GM + Y D + + N M+ + IT+ ILE K FEG+ +VDVGGG
Sbjct: 156 FNKAYGMSAFEYHGTDPRFNKVFNNGMS-NHSTITMKKILETYKG-FEGLTSLVDVGGGI 215
Query: 217 GTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFDSIPKADAALLMWVLHDW 276
G L MIV +P ++GINFDLPHVI A G++HVGGDMF S+PK DA + W+ HDW
Sbjct: 216 GATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDW 275
Query: 277 DDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLSDVGLM-LDMVMMAHTNKGKER 335
DE C+K LKNC +++P GKVI+ + ++ E +++LS ++ +D +M+AH GKER
Sbjct: 276 SDEHCVKFLKNCYESLPE-DGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKER 335
BLAST of MS028268 vs. TAIR 10
Match:
AT1G51990.1 (O-methyltransferase family protein )
HSP 1 Score: 177.2 bits (448), Expect = 2.4e-44
Identity = 112/318 (35.22%), Postives = 183/318 (57.55%), Query Frame = 0
Query: 36 VVKCAIELKI------ADAIETQTSPMTLSQLSSALN-CSPPLLHRILRFLVRRGI---- 95
+VK A EL + A + + SP+ L+ +++ N +P ++ R+LRFLV +
Sbjct: 31 IVKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTCK 90
Query: 96 -FREQTTTENLTGYSHTPLSRLLTT-----SMAPFLLLESSPTMLAPWQKLSTCVKEGNG 155
+++ E+ Y + + L S+AP++L + W L+ ++EG G
Sbjct: 91 LVKDEEGRES-RAYGLGKVGKKLIKDEDGFSIAPYVLAGCTKAKGGVWSYLTEAIQEG-G 150
Query: 156 IAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMITVPAILEGCKEIFEGVGCVVDVG 215
+ +E A+ ++ Y + + NE+M + I + ILE FEGV VDVG
Sbjct: 151 ASAWERANEALIFEYMKKNENLKKIFNESMT-NHTSIVMKKILENYIG-FEGVSDFVDVG 210
Query: 216 GGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFDSIPKADAALLMWVL 275
G G+NL+ I+ +P I+GINFDLPH++ A + GV+H+GGDMFD IP+ + L+ W+L
Sbjct: 211 GSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWIL 270
Query: 276 HDWDDEECIKILKNCKDAIPRTTGKVIIVDTVI-NEKEENNLSDV-GLMLDMVMMAHTNK 335
HDW+DE+C++ILKNCK A+P TG++I+++ ++ E E +L+ L D+ MM+ T+
Sbjct: 271 HDWNDEKCVEILKNCKKALPE-TGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSG 330
BLAST of MS028268 vs. TAIR 10
Match:
AT1G51990.2 (O-methyltransferase family protein )
HSP 1 Score: 175.6 bits (444), Expect = 6.9e-44
Identity = 111/318 (34.91%), Postives = 182/318 (57.23%), Query Frame = 0
Query: 36 VVKCAIELKI------ADAIETQTSPMTLSQLSSALN-CSPPLLHRILRFLVRRGI---- 95
+VK A EL + A + + SP+ L+ +++ N +P ++ R+LRFLV +
Sbjct: 31 IVKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTCK 90
Query: 96 -FREQTTTENLTGYSHTPLSRLLTT-----SMAPFLLLESSPTMLAPWQKLSTCVKEGNG 155
+++ E+ Y + + L S+AP++L + W + ++EG G
Sbjct: 91 LVKDEEGRES-RAYGLGKVGKKLIKDEDGFSIAPYVLAGCTKAKGGVWYNVQHAIQEG-G 150
Query: 156 IAPFEAAHGMDVWSYAAGDAVHSALINEAMACSGRMITVPAILEGCKEIFEGVGCVVDVG 215
+ +E A+ ++ Y + + NE+M + I + ILE FEGV VDVG
Sbjct: 151 ASAWERANEALIFEYMKKNENLKKIFNESMT-NHTSIVMKKILENYIG-FEGVSDFVDVG 210
Query: 216 GGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFDSIPKADAALLMWVL 275
G G+NL+ I+ +P I+GINFDLPH++ A + GV+H+GGDMFD IP+ + L+ W+L
Sbjct: 211 GSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWIL 270
Query: 276 HDWDDEECIKILKNCKDAIPRTTGKVIIVDTVI-NEKEENNLSDV-GLMLDMVMMAHTNK 335
HDW+DE+C++ILKNCK A+P TG++I+++ ++ E E +L+ L D+ MM+ T+
Sbjct: 271 HDWNDEKCVEILKNCKKALPE-TGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSG 330
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022135680.1 | 6.0e-199 | 98.58 | (R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia] | [more] |
| XP_022135713.1 | 1.4e-147 | 72.55 | (R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia] | [more] |
| XP_022136086.1 | 9.0e-147 | 73.39 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica char... | [more] |
| XP_038888665.1 | 1.4e-144 | 73.95 | acetylserotonin O-methyltransferase-like [Benincasa hispida] | [more] |
| XP_023554119.1 | 1.2e-143 | 71.91 | (RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9T003 | 3.3e-99 | 52.03 | Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1... | [more] |
| Q6WUC2 | 3.8e-71 | 42.90 | (R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=... | [more] |
| B0ZB56 | 6.0e-69 | 43.21 | Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1 | [more] |
| Q7XB10 | 1.5e-67 | 39.71 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferu... | [more] |
| Q9LEL5 | 5.6e-67 | 40.00 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1C1Q0 | 2.9e-199 | 98.58 | (R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO... | [more] |
| A0A6J1C5N1 | 6.7e-148 | 72.55 | (R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO... | [more] |
| A0A6J1C2I8 | 4.4e-147 | 73.39 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica ch... | [more] |
| A0A6J1GLX2 | 2.3e-140 | 70.79 | (RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L... | [more] |
| A0A5D3DJ08 | 2.6e-139 | 70.64 | (R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=11946... | [more] |
