MS028233 (gene) Bitter gourd (TR) v1

BLAST of MS028233 vs. NCBI nr
Match: XP_022135666.1 (BURP domain protein USPL1-like [Momordica charantia])

HSP 1 Score: 563.5 bits (1451), Expect = 1.0e-156
Identity = 276/290 (95.17%), Postives = 282/290 (97.24%), Query Frame = 0

Query: 5   KICTELLNEVTTISLDFAEALAAVGEKSKQMENLNDHIEASVIKMVTQMEDNFHSSHMEH 64
           ++ + L+  +  + L FAEALAAVGEKSKQMENLNDHIEASVIKMVTQMEDNFHSSHMEH
Sbjct: 4   RVASSLIILLHLLLLMFAEALAAVGEKSKQMENLNDHIEASVIKMVTQMEDNFHSSHMEH 63

Query: 65  TNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSF 124
           TNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSF
Sbjct: 64  TNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSF 123

Query: 125 PLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLT 184
           PLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLT
Sbjct: 124 PLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLT 183

Query: 185 KSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRV 244
           KSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRV
Sbjct: 184 KSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRV 243

Query: 245 QAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTPMPA 295
           QAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTPMPA
Sbjct: 244 QAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTPMPA 293

BLAST of MS028233 vs. NCBI nr
Match: XP_023531754.1 (BURP domain protein USPL1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418.7 bits (1075), Expect = 4.2e-113
Identity = 208/281 (74.02%), Postives = 239/281 (85.05%), Query Frame = 0

Query: 23  EALAAVGEKSKQMENLNDHIEAS-----VIKMVTQM--EDNFH-----SSHMEHTNPQLI 82
           +  + + EK+KQ+E LNDHIEA+     +IKMVT+M  + N H     SSHM+H NP LI
Sbjct: 26  QGFSEIEEKNKQIE-LNDHIEATIKAKPIIKMVTKMANDHNSHDAHSSSSHMDHINPLLI 85

Query: 83  VFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSFPLNSPQ 142
           VFFTINDLKQG+KLPIYFPKRDPSKSPP LPKE+ADPIPFSLKKLPQILS+FS+P NSPQ
Sbjct: 86  VFFTINDLKQGKKLPIYFPKRDPSKSPPLLPKEQADPIPFSLKKLPQILSYFSYPSNSPQ 145

Query: 143 SQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLTKSLVHF 202
           +QAVKETL+QCELKPIKGETKFCATSMESMV+FV TA G K + +++LTTSHLTKS VH 
Sbjct: 146 AQAVKETLEQCELKPIKGETKFCATSMESMVNFVRTAFGPK-THYEVLTTSHLTKSNVHL 205

Query: 203 QNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRVQAMAVC 262
           QNYT   APE IAAP+LVACH MPY YAVYYCHYQ+GDN+VLKI LEG+N DRV+A+A+C
Sbjct: 206 QNYTFIEAPEEIAAPRLVACHTMPYAYAVYYCHYQKGDNNVLKIQLEGENGDRVEALAIC 265

Query: 263 HMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTP 292
           HMDTSQWSPSHP+F+VLK +PG  PVCHFFPADDFVWIP P
Sbjct: 266 HMDTSQWSPSHPSFQVLKIQPGTIPVCHFFPADDFVWIPVP 304

BLAST of MS028233 vs. NCBI nr
Match: XP_022927168.1 (BURP domain protein USPL1-like isoform X1 [Cucurbita moschata] >KAG6588446.1 BURP domain protein USPL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 418.7 bits (1075), Expect = 4.2e-113
Identity = 208/281 (74.02%), Postives = 239/281 (85.05%), Query Frame = 0

Query: 23  EALAAVGEKSKQMENLNDHIEAS-----VIKMVTQM--EDNFH-----SSHMEHTNPQLI 82
           +  + + EK+KQ+E LNDHIEA+     +IKMVT+M  + N H     SSHM+H NP LI
Sbjct: 26  QTFSEIEEKNKQIE-LNDHIEATFKPKPIIKMVTKMANDHNSHDTHSSSSHMDHINPLLI 85

Query: 83  VFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSFPLNSPQ 142
           VFFTINDLKQG+KLPIYFPKRDPSKSPP LPKE+ADPIPFSLKKLPQILS+FS+P NSPQ
Sbjct: 86  VFFTINDLKQGKKLPIYFPKRDPSKSPPLLPKEQADPIPFSLKKLPQILSYFSYPSNSPQ 145

Query: 143 SQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLTKSLVHF 202
           +QAVKETL+QCELKPIKGETKFCATSMESMV+FV TA G K + +++LTTSHLTKS VH 
Sbjct: 146 AQAVKETLEQCELKPIKGETKFCATSMESMVNFVRTAFGPK-THYEVLTTSHLTKSNVHL 205

Query: 203 QNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRVQAMAVC 262
           QNYT   AP+ IAAP+LVACH MPY YAVYYCHYQEGDN+VLKI LEG+N DRV+A+A+C
Sbjct: 206 QNYTFIEAPKEIAAPRLVACHTMPYAYAVYYCHYQEGDNNVLKIQLEGENGDRVEALAIC 265

Query: 263 HMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTP 292
           HMDTSQWSPSHP+F+VLK +PG  PVCHFFPADDFVWIP P
Sbjct: 266 HMDTSQWSPSHPSFQVLKIQPGTIPVCHFFPADDFVWIPVP 304

BLAST of MS028233 vs. NCBI nr
Match: XP_022971234.1 (BURP domain protein USPL1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 417.5 bits (1072), Expect = 9.3e-113
Identity = 208/281 (74.02%), Postives = 239/281 (85.05%), Query Frame = 0

Query: 23  EALAAVGEKSKQMENLNDHIEAS-----VIKMVTQM--EDNFH-----SSHMEHTNPQLI 82
           +A + + EK+KQ+E LNDHIEA+     +IKMVT+M  + N H     SSHM+H NP L 
Sbjct: 26  QAFSEIEEKNKQIE-LNDHIEATFKPKPIIKMVTKMANDHNSHDTHSSSSHMDHINPLLR 85

Query: 83  VFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSFPLNSPQ 142
           VFFTINDLKQG+KLPIYFPKRDPSKSPP LPKE+ADPIPFSLKKLPQILS+FS+P NSPQ
Sbjct: 86  VFFTINDLKQGKKLPIYFPKRDPSKSPPLLPKEQADPIPFSLKKLPQILSYFSYPSNSPQ 145

Query: 143 SQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLTKSLVHF 202
           +QAVKETL+QCELKPIKGETKFCATSMESMV+FV TA G K + +++LTTSHLTKS VH 
Sbjct: 146 AQAVKETLEQCELKPIKGETKFCATSMESMVNFVRTAFGPK-THYEVLTTSHLTKSNVHL 205

Query: 203 QNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRVQAMAVC 262
           QNYT   AP+ IAAP+LVACH MPY YAVYYCHYQEGDN+VLKI LEG+N DRV+A+A+C
Sbjct: 206 QNYTFIEAPKEIAAPRLVACHTMPYAYAVYYCHYQEGDNNVLKIQLEGENGDRVEALAIC 265

Query: 263 HMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTP 292
           HMDTSQWSPSHP+F+VLK +PG  PVCHFFPADDFVWIP P
Sbjct: 266 HMDTSQWSPSHPSFQVLKIQPGTIPVCHFFPADDFVWIPVP 304

BLAST of MS028233 vs. NCBI nr
Match: XP_022927177.1 (BURP domain protein USPL1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 399.8 bits (1026), Expect = 2.0e-107
Identity = 194/250 (77.60%), Postives = 218/250 (87.20%), Query Frame = 0

Query: 49  MVTQM--EDNFH-----SSHMEHTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLP 108
           MVT+M  + N H     SSHM+H NP LIVFFTINDLKQG+KLPIYFPKRDPSKSPP LP
Sbjct: 1   MVTKMANDHNSHDTHSSSSHMDHINPLLIVFFTINDLKQGKKLPIYFPKRDPSKSPPLLP 60

Query: 109 KEKADPIPFSLKKLPQILSFFSFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMV 168
           KE+ADPIPFSLKKLPQILS+FS+P NSPQ+QAVKETL+QCELKPIKGETKFCATSMESMV
Sbjct: 61  KEQADPIPFSLKKLPQILSYFSYPSNSPQAQAVKETLEQCELKPIKGETKFCATSMESMV 120

Query: 169 DFVGTALGTKPSSFQLLTTSHLTKSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYY 228
           +FV TA G K + +++LTTSHLTKS VH QNYT   AP+ IAAP+LVACH MPY YAVYY
Sbjct: 121 NFVRTAFGPK-THYEVLTTSHLTKSNVHLQNYTFIEAPKEIAAPRLVACHTMPYAYAVYY 180

Query: 229 CHYQEGDNSVLKIPLEGDNSDRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFP 288
           CHYQEGDN+VLKI LEG+N DRV+A+A+CHMDTSQWSPSHP+F+VLK +PG  PVCHFFP
Sbjct: 181 CHYQEGDNNVLKIQLEGENGDRVEALAICHMDTSQWSPSHPSFQVLKIQPGTIPVCHFFP 240

Query: 289 ADDFVWIPTP 292
           ADDFVWIP P
Sbjct: 241 ADDFVWIPVP 249

BLAST of MS028233 vs. ExPASy Swiss-Prot
Match: Q9XI99 (BURP domain protein USPL1 OS=Arabidopsis thaliana OX=3702 GN=USPL1 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.9e-54
Identity = 108/231 (46.75%), Postives = 143/231 (61.90%), Query Frame = 0

Query: 63  EHTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFF 122
           E  +P L ++FT+NDLK G KL IYF K D  K PP L +++AD IPF+  KL  +L  F
Sbjct: 49  EFDDPSLYMYFTLNDLKLGTKLLIYFYKNDLQKLPPLLTRQQADLIPFTKSKLDFLLDHF 108

Query: 123 SFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSH 182
           S   +SPQ +A+KETL  C+ K I+GE KFC TS+ES++D V   +G      +++TT  
Sbjct: 109 SITKDSPQGKAIKETLGHCDAKAIEGEHKFCGTSLESLIDLVKKTMGYN-VDLKVMTTKV 168

Query: 183 LTKS----LVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPL-E 242
           +  +         NYT   AP+ +   K++ CH MPYPYAVYYCH  +G + V ++ L  
Sbjct: 169 MVPAQNSISYALHNYTFVEAPKELVGIKMLGCHRMPYPYAVYYCHGHKGGSRVFEVNLVT 228

Query: 243 GDNSDRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWI 289
            D   RV   AVCHMDTS W   H AF+VLK EP + PVCHFFP D+ VW+
Sbjct: 229 DDGRQRVVGPAVCHMDTSTWDADHVAFKVLKMEPRSAPVCHFFPLDNIVWV 278

BLAST of MS028233 vs. ExPASy Swiss-Prot
Match: D2CZZ9 (BURP domain-containing protein BNM2C OS=Brassica napus OX=3708 GN=BNM2C PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.9e-52
Identity = 104/231 (45.02%), Postives = 143/231 (61.90%), Query Frame = 0

Query: 63  EHTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFF 122
           E  +P + +FF I+DLK G KLPIYF K D  K PP L +++AD IPFS   L  +L+ F
Sbjct: 50  EFEDPTMYMFFKISDLKLGTKLPIYFNKNDLRKVPPLLTRQEADLIPFSESNLDFLLNHF 109

Query: 123 SFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSH 182
           S   +SPQ +A+KETLQ+C+ K I+GE KFC TS+ESM+D     + +  +  +++TT  
Sbjct: 110 SISKDSPQGEAMKETLQRCDFKAIEGEYKFCGTSLESMLDLAKKTIASN-ADLKVMTTKV 169

Query: 183 L----TKSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEG 242
           +     +      NYT +  P+ +   K++ CH MPYPY VYYCH  +    V ++ L  
Sbjct: 170 MVPDQNRISYALHNYTFAEVPKELDGIKVLGCHRMPYPYVVYYCHGHKSGTKVFEVNLMS 229

Query: 243 DNS-DRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWI 289
           D+    V   AVCHMDTS W+  H AF+VLK EP + PVCHFFP D+ VW+
Sbjct: 230 DDGIQLVVGPAVCHMDTSMWNADHVAFKVLKIEPRSAPVCHFFPLDNIVWV 279

BLAST of MS028233 vs. ExPASy Swiss-Prot
Match: O65009 (BURP domain-containing protein BNM2A OS=Brassica napus OX=3708 GN=BNM2A PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 7.3e-52
Identity = 103/231 (44.59%), Postives = 143/231 (61.90%), Query Frame = 0

Query: 63  EHTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFF 122
           E  +P + +FF I+DLK G KLPIYF K D  K PP L +++AD IPFS   L  +L+ F
Sbjct: 51  EFEDPTMYMFFKISDLKLGTKLPIYFNKNDLRKVPPLLTRQEADLIPFSESNLDFLLNHF 110

Query: 123 SFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSH 182
           S   +SPQ +A+KETL++C+ K I+GE KFC TS+ESM+D     + +  +  +++TT  
Sbjct: 111 SISKDSPQGKAMKETLKRCDFKAIEGEYKFCGTSLESMLDLAKKTIASN-ADLKVMTTKV 170

Query: 183 L----TKSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEG 242
           +     +      NYT +  P+ +   K++ CH MPYPY VYYCH  +    V ++ L  
Sbjct: 171 MVPDQNRISYALHNYTFAEVPKELDGIKVLGCHRMPYPYVVYYCHGHKSGTKVFEVNLMS 230

Query: 243 DNS-DRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWI 289
           D+    V   AVCHMDTS W+  H AF+VLK EP + PVCHFFP D+ VW+
Sbjct: 231 DDGIQLVVGPAVCHMDTSMWNADHVAFKVLKIEPRSAPVCHFFPLDNIVWV 280

BLAST of MS028233 vs. ExPASy Swiss-Prot
Match: B9G9L9 (BURP domain-containing protein 17 OS=Oryza sativa subsp. japonica OX=39947 GN=BURP17 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.2e-43
Identity = 100/253 (39.53%), Postives = 140/253 (55.34%), Query Frame = 0

Query: 42  IEASVIKMVTQMEDNFHSSHMEH----TNPQLIVFFTINDLKQGQKL--PIYFPKRDPSK 101
           + A+ +   T  + N+ SS +E      +P + +FF   +L+QG+K+   ++F     + 
Sbjct: 329 LPATELVHYTDGQRNYKSSVLETPELLKDPDMALFFLEKNLQQGKKINNALHFANLLATT 388

Query: 102 SPPFLPKEKADPIPFSLKKLPQILSFFSFPLNSPQSQAVKETLQQCELKPIKGETKFCAT 161
           +  FLP+ KAD IPFS K+LP+IL  F     S  +  +  TLQ CEL   KGE K CAT
Sbjct: 389 NSKFLPRGKADSIPFSSKELPEILDRFGVRPGSDDAAEMSATLQDCELPANKGEKKACAT 448

Query: 162 SMESMVDFVGTALGTKP-SSFQLLTTSHLTKSLVHFQNYTISGAPEVIAAPKLVACHAMP 221
           S+ES+VDFV ++ G     +   +  S   +S    Q+YT+SG   +    +L+ACH   
Sbjct: 449 SLESIVDFVTSSFGASDVDAASTVVLSKAVESSSLAQDYTVSGVRRMAGTGQLIACHPES 508

Query: 222 YPYAVYYCHYQEGDNSVLKIPLEGDNSDRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGAN 281
           YPYAV+ CH  E      K  L G +   V+A+AVCH DTS W+P H AF VL  +PG  
Sbjct: 509 YPYAVFMCHLTEATTRAYKASLVGKDGTAVEAVAVCHTDTSDWNPEHAAFHVLGVKPGTV 568

Query: 282 PVCHFFPADDFVW 288
           PVCHF   D  VW
Sbjct: 569 PVCHFMQPDAVVW 581

BLAST of MS028233 vs. ExPASy Swiss-Prot
Match: Q942D4 (BURP domain-containing protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=BURP3 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.2e-41
Identity = 92/227 (40.53%), Postives = 132/227 (58.15%), Query Frame = 0

Query: 64  HTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFS 123
           H +P + +FF   DL  G+ + ++F      +   FLP+ +AD +PFS +K+P+ILS FS
Sbjct: 205 HDDPNVALFFLEKDLHPGKTMAVHFTATTAGEK--FLPRSEADAMPFSSEKVPEILSRFS 264

Query: 124 FPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHL 183
               S ++  + +TL+ CE  P +GE K CATS+ESMVDF  ++LGT   S     ++ +
Sbjct: 265 VKPGSVEAAEMAQTLRDCEAPPAQGERKACATSLESMVDFATSSLGT---SHVRAASTVV 324

Query: 184 TKSLVHFQNYTISGAPEVIAA---PKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDN 243
            K     Q YT++      A     +LVACHA PY YAV+ CH      +   + + G +
Sbjct: 325 GKEGSPEQEYTVTAVKRAAAGGDQDQLVACHAEPYAYAVFACHLTRATRA-YAVSMAGRD 384

Query: 244 SDRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVW 288
              V+A+AVCH DT+ W+P H AF+VLK +PG  PVCHF P D  VW
Sbjct: 385 GTGVEAVAVCHADTAGWNPKHVAFQVLKVKPGTVPVCHFLPQDHVVW 425

BLAST of MS028233 vs. ExPASy TrEMBL
Match: A0A6J1C1E3 (BURP domain protein USPL1-like OS=Momordica charantia OX=3673 GN=LOC111007566 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.1e-157
Identity = 276/290 (95.17%), Postives = 282/290 (97.24%), Query Frame = 0

Query: 5   KICTELLNEVTTISLDFAEALAAVGEKSKQMENLNDHIEASVIKMVTQMEDNFHSSHMEH 64
           ++ + L+  +  + L FAEALAAVGEKSKQMENLNDHIEASVIKMVTQMEDNFHSSHMEH
Sbjct: 4   RVASSLIILLHLLLLMFAEALAAVGEKSKQMENLNDHIEASVIKMVTQMEDNFHSSHMEH 63

Query: 65  TNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSF 124
           TNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSF
Sbjct: 64  TNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSF 123

Query: 125 PLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLT 184
           PLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLT
Sbjct: 124 PLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLT 183

Query: 185 KSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRV 244
           KSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRV
Sbjct: 184 KSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRV 243

Query: 245 QAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTPMPA 295
           QAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTPMPA
Sbjct: 244 QAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTPMPA 293

BLAST of MS028233 vs. ExPASy TrEMBL
Match: A0A6J1EK95 (BURP domain protein USPL1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434097 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 2.0e-113
Identity = 208/281 (74.02%), Postives = 239/281 (85.05%), Query Frame = 0

Query: 23  EALAAVGEKSKQMENLNDHIEAS-----VIKMVTQM--EDNFH-----SSHMEHTNPQLI 82
           +  + + EK+KQ+E LNDHIEA+     +IKMVT+M  + N H     SSHM+H NP LI
Sbjct: 26  QTFSEIEEKNKQIE-LNDHIEATFKPKPIIKMVTKMANDHNSHDTHSSSSHMDHINPLLI 85

Query: 83  VFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSFPLNSPQ 142
           VFFTINDLKQG+KLPIYFPKRDPSKSPP LPKE+ADPIPFSLKKLPQILS+FS+P NSPQ
Sbjct: 86  VFFTINDLKQGKKLPIYFPKRDPSKSPPLLPKEQADPIPFSLKKLPQILSYFSYPSNSPQ 145

Query: 143 SQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLTKSLVHF 202
           +QAVKETL+QCELKPIKGETKFCATSMESMV+FV TA G K + +++LTTSHLTKS VH 
Sbjct: 146 AQAVKETLEQCELKPIKGETKFCATSMESMVNFVRTAFGPK-THYEVLTTSHLTKSNVHL 205

Query: 203 QNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRVQAMAVC 262
           QNYT   AP+ IAAP+LVACH MPY YAVYYCHYQEGDN+VLKI LEG+N DRV+A+A+C
Sbjct: 206 QNYTFIEAPKEIAAPRLVACHTMPYAYAVYYCHYQEGDNNVLKIQLEGENGDRVEALAIC 265

Query: 263 HMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTP 292
           HMDTSQWSPSHP+F+VLK +PG  PVCHFFPADDFVWIP P
Sbjct: 266 HMDTSQWSPSHPSFQVLKIQPGTIPVCHFFPADDFVWIPVP 304

BLAST of MS028233 vs. ExPASy TrEMBL
Match: A0A6J1I7Z5 (BURP domain protein USPL1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470018 PE=4 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 4.5e-113
Identity = 208/281 (74.02%), Postives = 239/281 (85.05%), Query Frame = 0

Query: 23  EALAAVGEKSKQMENLNDHIEAS-----VIKMVTQM--EDNFH-----SSHMEHTNPQLI 82
           +A + + EK+KQ+E LNDHIEA+     +IKMVT+M  + N H     SSHM+H NP L 
Sbjct: 26  QAFSEIEEKNKQIE-LNDHIEATFKPKPIIKMVTKMANDHNSHDTHSSSSHMDHINPLLR 85

Query: 83  VFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSFPLNSPQ 142
           VFFTINDLKQG+KLPIYFPKRDPSKSPP LPKE+ADPIPFSLKKLPQILS+FS+P NSPQ
Sbjct: 86  VFFTINDLKQGKKLPIYFPKRDPSKSPPLLPKEQADPIPFSLKKLPQILSYFSYPSNSPQ 145

Query: 143 SQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLTKSLVHF 202
           +QAVKETL+QCELKPIKGETKFCATSMESMV+FV TA G K + +++LTTSHLTKS VH 
Sbjct: 146 AQAVKETLEQCELKPIKGETKFCATSMESMVNFVRTAFGPK-THYEVLTTSHLTKSNVHL 205

Query: 203 QNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRVQAMAVC 262
           QNYT   AP+ IAAP+LVACH MPY YAVYYCHYQEGDN+VLKI LEG+N DRV+A+A+C
Sbjct: 206 QNYTFIEAPKEIAAPRLVACHTMPYAYAVYYCHYQEGDNNVLKIQLEGENGDRVEALAIC 265

Query: 263 HMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWIPTP 292
           HMDTSQWSPSHP+F+VLK +PG  PVCHFFPADDFVWIP P
Sbjct: 266 HMDTSQWSPSHPSFQVLKIQPGTIPVCHFFPADDFVWIPVP 304

BLAST of MS028233 vs. ExPASy TrEMBL
Match: A0A6J1EGF2 (BURP domain protein USPL1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434097 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 9.7e-108
Identity = 194/250 (77.60%), Postives = 218/250 (87.20%), Query Frame = 0

Query: 49  MVTQM--EDNFH-----SSHMEHTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLP 108
           MVT+M  + N H     SSHM+H NP LIVFFTINDLKQG+KLPIYFPKRDPSKSPP LP
Sbjct: 1   MVTKMANDHNSHDTHSSSSHMDHINPLLIVFFTINDLKQGKKLPIYFPKRDPSKSPPLLP 60

Query: 109 KEKADPIPFSLKKLPQILSFFSFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMV 168
           KE+ADPIPFSLKKLPQILS+FS+P NSPQ+QAVKETL+QCELKPIKGETKFCATSMESMV
Sbjct: 61  KEQADPIPFSLKKLPQILSYFSYPSNSPQAQAVKETLEQCELKPIKGETKFCATSMESMV 120

Query: 169 DFVGTALGTKPSSFQLLTTSHLTKSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYY 228
           +FV TA G K + +++LTTSHLTKS VH QNYT   AP+ IAAP+LVACH MPY YAVYY
Sbjct: 121 NFVRTAFGPK-THYEVLTTSHLTKSNVHLQNYTFIEAPKEIAAPRLVACHTMPYAYAVYY 180

Query: 229 CHYQEGDNSVLKIPLEGDNSDRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFP 288
           CHYQEGDN+VLKI LEG+N DRV+A+A+CHMDTSQWSPSHP+F+VLK +PG  PVCHFFP
Sbjct: 181 CHYQEGDNNVLKIQLEGENGDRVEALAICHMDTSQWSPSHPSFQVLKIQPGTIPVCHFFP 240

Query: 289 ADDFVWIPTP 292
           ADDFVWIP P
Sbjct: 241 ADDFVWIPVP 249

BLAST of MS028233 vs. ExPASy TrEMBL
Match: A0A6J1I803 (BURP domain protein USPL1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470018 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 6.3e-107
Identity = 193/250 (77.20%), Postives = 217/250 (86.80%), Query Frame = 0

Query: 49  MVTQM--EDNFH-----SSHMEHTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLP 108
           MVT+M  + N H     SSHM+H NP L VFFTINDLKQG+KLPIYFPKRDPSKSPP LP
Sbjct: 1   MVTKMANDHNSHDTHSSSSHMDHINPLLRVFFTINDLKQGKKLPIYFPKRDPSKSPPLLP 60

Query: 109 KEKADPIPFSLKKLPQILSFFSFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMV 168
           KE+ADPIPFSLKKLPQILS+FS+P NSPQ+QAVKETL+QCELKPIKGETKFCATSMESMV
Sbjct: 61  KEQADPIPFSLKKLPQILSYFSYPSNSPQAQAVKETLEQCELKPIKGETKFCATSMESMV 120

Query: 169 DFVGTALGTKPSSFQLLTTSHLTKSLVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYY 228
           +FV TA G K + +++LTTSHLTKS VH QNYT   AP+ IAAP+LVACH MPY YAVYY
Sbjct: 121 NFVRTAFGPK-THYEVLTTSHLTKSNVHLQNYTFIEAPKEIAAPRLVACHTMPYAYAVYY 180

Query: 229 CHYQEGDNSVLKIPLEGDNSDRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFP 288
           CHYQEGDN+VLKI LEG+N DRV+A+A+CHMDTSQWSPSHP+F+VLK +PG  PVCHFFP
Sbjct: 181 CHYQEGDNNVLKIQLEGENGDRVEALAICHMDTSQWSPSHPSFQVLKIQPGTIPVCHFFP 240

Query: 289 ADDFVWIPTP 292
           ADDFVWIP P
Sbjct: 241 ADDFVWIPVP 249

BLAST of MS028233 vs. TAIR 10
Match: AT1G49320.1 (unknown seed protein like 1 )

HSP 1 Score: 212.6 bits (540), Expect = 4.2e-55
Identity = 108/231 (46.75%), Postives = 143/231 (61.90%), Query Frame = 0

Query: 63  EHTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFF 122
           E  +P L ++FT+NDLK G KL IYF K D  K PP L +++AD IPF+  KL  +L  F
Sbjct: 49  EFDDPSLYMYFTLNDLKLGTKLLIYFYKNDLQKLPPLLTRQQADLIPFTKSKLDFLLDHF 108

Query: 123 SFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSH 182
           S   +SPQ +A+KETL  C+ K I+GE KFC TS+ES++D V   +G      +++TT  
Sbjct: 109 SITKDSPQGKAIKETLGHCDAKAIEGEHKFCGTSLESLIDLVKKTMGYN-VDLKVMTTKV 168

Query: 183 LTKS----LVHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPL-E 242
           +  +         NYT   AP+ +   K++ CH MPYPYAVYYCH  +G + V ++ L  
Sbjct: 169 MVPAQNSISYALHNYTFVEAPKELVGIKMLGCHRMPYPYAVYYCHGHKGGSRVFEVNLVT 228

Query: 243 GDNSDRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVWI 289
            D   RV   AVCHMDTS W   H AF+VLK EP + PVCHFFP D+ VW+
Sbjct: 229 DDGRQRVVGPAVCHMDTSTWDADHVAFKVLKMEPRSAPVCHFFPLDNIVWV 278

BLAST of MS028233 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 168.7 bits (426), Expect = 7.0e-42
Identity = 87/226 (38.50%), Postives = 133/226 (58.85%), Query Frame = 0

Query: 64  HTNPQLIVFFTINDLKQGQKLPIYFPKRDP-SKSPPFLPKEKADPIPFSLKKLPQILSFF 123
           H +P   +FF   DL +G+++ + F   D       FLP+ +A+ +PF  +K  + L  F
Sbjct: 168 HDDPNAALFFLEKDLVRGKEMNVRFNAEDGYGGKTAFLPRGEAETVPFGSEKFSETLKRF 227

Query: 124 SFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSH 183
           S    S +++ +K+T+++CE + + GE K+CATS+ESMVDF  + LG          ++ 
Sbjct: 228 SVEAGSEEAEMMKKTIEECEARKVSGEEKYCATSLESMVDFSVSKLG---KYHVRAVSTE 287

Query: 184 LTKSLVHFQNYTISGA-PEVIAAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNS 243
           + K     Q Y I+ A  + ++  K V CH   YP+AV+YCH +    +V  +PLEG+N 
Sbjct: 288 VAKKNAPMQKYKIAAAGVKKLSDDKSVVCHKQKYPFAVFYCH-KAMMTTVYAVPLEGENG 347

Query: 244 DRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVW 288
            R +A+AVCH +TS W+P+H AF+VLK +PG  PVCHF P    VW
Sbjct: 348 MRAKAVAVCHKNTSAWNPNHLAFKVLKVKPGTVPVCHFLPETHVVW 389

BLAST of MS028233 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 115.9 bits (289), Expect = 5.4e-26
Identity = 77/220 (35.00%), Postives = 106/220 (48.18%), Query Frame = 0

Query: 72  FFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSFPLNSPQS 131
           FF  + LK+G  +P+   K D      FLP+     +PFS  KL +I   F    NS   
Sbjct: 411 FFRESSLKEGTVIPMPDIK-DKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMG 470

Query: 132 QAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLTKSLVHFQ 191
             + + + +CE  P  GETK C  S E M+DF  + LG    S  L TT ++  S    +
Sbjct: 471 GIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLG---RSVVLRTTENVAGS----K 530

Query: 192 NYTISGAPEVIAAPKL---VACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRV-QAM 251
              + G    I   KL   V+CH   YPY +YYCH            LE ++  ++   +
Sbjct: 531 EKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGI 590

Query: 252 AVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVW 288
           A+CHMDTS W PSH AF  L ++PG   VCH+   +D  W
Sbjct: 591 AICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNW 622

BLAST of MS028233 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 115.9 bits (289), Expect = 5.4e-26
Identity = 77/220 (35.00%), Postives = 106/220 (48.18%), Query Frame = 0

Query: 72  FFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSFPLNSPQS 131
           FF  + LK+G  +P+   K D      FLP+     +PFS  KL +I   F    NS   
Sbjct: 411 FFRESSLKEGTVIPMPDIK-DKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMG 470

Query: 132 QAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLTKSLVHFQ 191
             + + + +CE  P  GETK C  S E M+DF  + LG    S  L TT ++  S    +
Sbjct: 471 GIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLG---RSVVLRTTENVAGS----K 530

Query: 192 NYTISGAPEVIAAPKL---VACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRV-QAM 251
              + G    I   KL   V+CH   YPY +YYCH            LE ++  ++   +
Sbjct: 531 EKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGI 590

Query: 252 AVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVW 288
           A+CHMDTS W PSH AF  L ++PG   VCH+   +D  W
Sbjct: 591 AICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNW 622

BLAST of MS028233 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 99.8 bits (247), Expect = 4.0e-21
Identity = 67/220 (30.45%), Postives = 99/220 (45.00%), Query Frame = 0

Query: 72  FFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKADPIPFSLKKLPQILSFFSFPLNSPQS 131
           FF    LK+G  + +   K D      FLP+     +PFS   + +I   F    NS  +
Sbjct: 409 FFREAMLKEGTLMQMPDIK-DKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMA 468

Query: 132 QAVKETLQQCELKPIKGETKFCATSMESMVDFVGTALGTKPSSFQLLTTSHLTKSLVHFQ 191
             +   + +CE     GETK C  S E M+DF  + LG              T+++V  +
Sbjct: 469 GIISSAVSECERPASHGETKRCVGSAEDMIDFATSVLGRG-------VVVRTTENVVGSK 528

Query: 192 NYTISGAPEVI---AAPKLVACHAMPYPYAVYYCHYQEGDNSVLKIPLEGDNSDRV-QAM 251
              + G    I      + V+CH   YPY +YYCH            L+  + +++   +
Sbjct: 529 KKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGV 588

Query: 252 AVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPADDFVW 288
           A+CH+DTS WSPSH AF  L + PG   VCH+   +D  W
Sbjct: 589 AICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFENDMTW 620