MS027541 (gene) Bitter gourd (TR) v1

Overview
NameMS027541
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionDELLA protein
Locationscaffold313: 392088 .. 393923 (-)
RNA-Seq ExpressionMS027541
SyntenyMS027541
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGATGAAGATGAAGAGGGAGCATGAGGATGGGAGATCTACTGGTGGTGGTGGCGGTAGTGGTAGTGGTAGTGGTAGTGTCTATGGCGGCGGGGTTGTGAAGGCTGAGTGTTCTTCAATGTCGAGTGGGAAGATGAAGATGTGGGAGGAGGAGGACGGCGGCGGCGGGATGGATGAGTTGCTTGCTGTTTTGGGGTACAAGGTTCGTGCATCGGACATGGCGGATGTGGCGCAGAAGCTCGAGCAGCTTGAGATGGTGATGGGAACTGCTCAGGAAGATGGGATTTCTCATCTTTCTTGTGATACTGTTCATTATAATCCTTCTGATCTTTCTGGTTGGGTTCAAAATATGCTCTCTGAATTCAATAATTTCCCCAGCGATTTACAACATCGAGACCCTCTCTGTTTGCACAATTCCAATTCTCACTCCCAGATTCAATCTTCCCCTTCCCGTTTCTACAACGATGATTCTGAGTACGATTTGAGCGCCATTCCTGGCGTTGCGGCTCTGCCTCCCAAACATTCCTCTTCCTCCGCAGGGACCGAAACTAATTCACGGAAGCGATTGAAAACCAGTACCTCCTCGATTGGAGCGTCGTCTTCGCCGCCGTTCGTCGCTACGGCCGGGATTGTCTCTGAGCCTTCTCGACCTGTGGTGGTTGTGGACTCGCAAGAGGCCGGGATTCAGCTTGTTCACACGCTCATGGCCTGCGCCGAAGCGGTCCAGCAGGAGAATATGACGCTCGGCGATGCGTTGGTGAAGCACATCGGGTTGCTGATGGCGTCGCAGGCCGGCGCGATGAGGAAAGTCGCCACGTATTTCGCCGAAGCCCTAGCTCGTCGGATTTACAGAATATACTCTCCTCAAGACGGCCTCTACTCCGATCTCCTCCAGAATCATTTCTACGAGACCTGTCCGTACCTGAAATTCGCGCATTTCACCGCCAATCAGGCCATTCTCGAGGCCTTCGCGACGGCGAACAGAGTCCATGTAATCGATTTCAGTCTCAATCAAGGTATGCAGTGGCCAGTGCTGATGCAGGCGCTCGCATTACGCGTGGGAGGCCCGCCGGAGTTCCGGCTGACCGGAATCGGCCCGCCGCTGTCGGATAACAGCACCGATTCCTTGGAGCAGGTCGGTTGGAAGCTGTCGCGGACGGCGGAAGCCATCGGCGTGAAGTTCAAGTTCAATCATATCGTGTGCAGTAATCTGGAGGATCTGGACCCGGCGGCGTTGGACATCCTTCCGGCGGAGGTGGAGGCGGTGGCGGTGAACTCGGTGTTCGATCTCCACCGCTTATTGGCTCGACCTGGAGCGATCGAGAAGGTTTTGGGATCGATAAAGAGGATGAAGCCGAAGATCGTGACGATTGTGGAGCAGGAAGCGAACCACAACGGGCCGGTTTTCGTGGACCGGTTCACGGAGGCTCTGCATTACTACTCGAATCTGTTTGACTCGCTGGAGGGTTACTCGAACGGGTTCGAACCGGCGAATGAGGACGTGGTGATGTCGGAGTTTTATTTGGGGAGGCAAATATGCAACGTGGTGGCTTGTGAAGGAACGGACCGAGTCGAGCGACACGAGACACTGGCTCAGTGGCGAACTCGGATGGAATCGGCCGGGTTCGACCCGGTTCACATGGGCTCGAACGCGTTCAAGCAGGCCAGTATGCTTCTGGCCCTTTACGCCGGCGGCGAAGGGTACCGGGTGGAGGAGAATAACGGGTGTCTGATGCTGGGTTGGCATACTCGGCCGCTGATCGCCACCTCGGCCTGGCAGCTCGCCGCCGATGATTCTTGGCGAGTCGACTCAGTGGTTCGAGCGAGTGAATGA

mRNA sequence

ATGAAGATGAAGATGAAGAGGGAGCATGAGGATGGGAGATCTACTGGTGGTGGTGGCGGTAGTGGTAGTGGTAGTGGTAGTGTCTATGGCGGCGGGGTTGTGAAGGCTGAGTGTTCTTCAATGTCGAGTGGGAAGATGAAGATGTGGGAGGAGGAGGACGGCGGCGGCGGGATGGATGAGTTGCTTGCTGTTTTGGGGTACAAGGTTCGTGCATCGGACATGGCGGATGTGGCGCAGAAGCTCGAGCAGCTTGAGATGGTGATGGGAACTGCTCAGGAAGATGGGATTTCTCATCTTTCTTGTGATACTGTTCATTATAATCCTTCTGATCTTTCTGGTTGGGTTCAAAATATGCTCTCTGAATTCAATAATTTCCCCAGCGATTTACAACATCGAGACCCTCTCTGTTTGCACAATTCCAATTCTCACTCCCAGATTCAATCTTCCCCTTCCCGTTTCTACAACGATGATTCTGAGTACGATTTGAGCGCCATTCCTGGCGTTGCGGCTCTGCCTCCCAAACATTCCTCTTCCTCCGCAGGGACCGAAACTAATTCACGGAAGCGATTGAAAACCAGTACCTCCTCGATTGGAGCGTCGTCTTCGCCGCCGTTCGTCGCTACGGCCGGGATTGTCTCTGAGCCTTCTCGACCTGTGGTGGTTGTGGACTCGCAAGAGGCCGGGATTCAGCTTGTTCACACGCTCATGGCCTGCGCCGAAGCGGTCCAGCAGGAGAATATGACGCTCGGCGATGCGTTGGTGAAGCACATCGGGTTGCTGATGGCGTCGCAGGCCGGCGCGATGAGGAAAGTCGCCACGTATTTCGCCGAAGCCCTAGCTCGTCGGATTTACAGAATATACTCTCCTCAAGACGGCCTCTACTCCGATCTCCTCCAGAATCATTTCTACGAGACCTGTCCGTACCTGAAATTCGCGCATTTCACCGCCAATCAGGCCATTCTCGAGGCCTTCGCGACGGCGAACAGAGTCCATGTAATCGATTTCAGTCTCAATCAAGGTATGCAGTGGCCAGTGCTGATGCAGGCGCTCGCATTACGCGTGGGAGGCCCGCCGGAGTTCCGGCTGACCGGAATCGGCCCGCCGCTGTCGGATAACAGCACCGATTCCTTGGAGCAGGTCGGTTGGAAGCTGTCGCGGACGGCGGAAGCCATCGGCGTGAAGTTCAAGTTCAATCATATCGTGTGCAGTAATCTGGAGGATCTGGACCCGGCGGCGTTGGACATCCTTCCGGCGGAGGTGGAGGCGGTGGCGGTGAACTCGGTGTTCGATCTCCACCGCTTATTGGCTCGACCTGGAGCGATCGAGAAGGTTTTGGGATCGATAAAGAGGATGAAGCCGAAGATCGTGACGATTGTGGAGCAGGAAGCGAACCACAACGGGCCGGTTTTCGTGGACCGGTTCACGGAGGCTCTGCATTACTACTCGAATCTGTTTGACTCGCTGGAGGGTTACTCGAACGGGTTCGAACCGGCGAATGAGGACGTGGTGATGTCGGAGTTTTATTTGGGGAGGCAAATATGCAACGTGGTGGCTTGTGAAGGAACGGACCGAGTCGAGCGACACGAGACACTGGCTCAGTGGCGAACTCGGATGGAATCGGCCGGGTTCGACCCGGTTCACATGGGCTCGAACGCGTTCAAGCAGGCCAGTATGCTTCTGGCCCTTTACGCCGGCGGCGAAGGGTACCGGGTGGAGGAGAATAACGGGTGTCTGATGCTGGGTTGGCATACTCGGCCGCTGATCGCCACCTCGGCCTGGCAGCTCGCCGCCGATGATTCTTGGCGAGTCGACTCAGTGGTTCGAGCGAGTGAATGA

Coding sequence (CDS)

ATGAAGATGAAGATGAAGAGGGAGCATGAGGATGGGAGATCTACTGGTGGTGGTGGCGGTAGTGGTAGTGGTAGTGGTAGTGTCTATGGCGGCGGGGTTGTGAAGGCTGAGTGTTCTTCAATGTCGAGTGGGAAGATGAAGATGTGGGAGGAGGAGGACGGCGGCGGCGGGATGGATGAGTTGCTTGCTGTTTTGGGGTACAAGGTTCGTGCATCGGACATGGCGGATGTGGCGCAGAAGCTCGAGCAGCTTGAGATGGTGATGGGAACTGCTCAGGAAGATGGGATTTCTCATCTTTCTTGTGATACTGTTCATTATAATCCTTCTGATCTTTCTGGTTGGGTTCAAAATATGCTCTCTGAATTCAATAATTTCCCCAGCGATTTACAACATCGAGACCCTCTCTGTTTGCACAATTCCAATTCTCACTCCCAGATTCAATCTTCCCCTTCCCGTTTCTACAACGATGATTCTGAGTACGATTTGAGCGCCATTCCTGGCGTTGCGGCTCTGCCTCCCAAACATTCCTCTTCCTCCGCAGGGACCGAAACTAATTCACGGAAGCGATTGAAAACCAGTACCTCCTCGATTGGAGCGTCGTCTTCGCCGCCGTTCGTCGCTACGGCCGGGATTGTCTCTGAGCCTTCTCGACCTGTGGTGGTTGTGGACTCGCAAGAGGCCGGGATTCAGCTTGTTCACACGCTCATGGCCTGCGCCGAAGCGGTCCAGCAGGAGAATATGACGCTCGGCGATGCGTTGGTGAAGCACATCGGGTTGCTGATGGCGTCGCAGGCCGGCGCGATGAGGAAAGTCGCCACGTATTTCGCCGAAGCCCTAGCTCGTCGGATTTACAGAATATACTCTCCTCAAGACGGCCTCTACTCCGATCTCCTCCAGAATCATTTCTACGAGACCTGTCCGTACCTGAAATTCGCGCATTTCACCGCCAATCAGGCCATTCTCGAGGCCTTCGCGACGGCGAACAGAGTCCATGTAATCGATTTCAGTCTCAATCAAGGTATGCAGTGGCCAGTGCTGATGCAGGCGCTCGCATTACGCGTGGGAGGCCCGCCGGAGTTCCGGCTGACCGGAATCGGCCCGCCGCTGTCGGATAACAGCACCGATTCCTTGGAGCAGGTCGGTTGGAAGCTGTCGCGGACGGCGGAAGCCATCGGCGTGAAGTTCAAGTTCAATCATATCGTGTGCAGTAATCTGGAGGATCTGGACCCGGCGGCGTTGGACATCCTTCCGGCGGAGGTGGAGGCGGTGGCGGTGAACTCGGTGTTCGATCTCCACCGCTTATTGGCTCGACCTGGAGCGATCGAGAAGGTTTTGGGATCGATAAAGAGGATGAAGCCGAAGATCGTGACGATTGTGGAGCAGGAAGCGAACCACAACGGGCCGGTTTTCGTGGACCGGTTCACGGAGGCTCTGCATTACTACTCGAATCTGTTTGACTCGCTGGAGGGTTACTCGAACGGGTTCGAACCGGCGAATGAGGACGTGGTGATGTCGGAGTTTTATTTGGGGAGGCAAATATGCAACGTGGTGGCTTGTGAAGGAACGGACCGAGTCGAGCGACACGAGACACTGGCTCAGTGGCGAACTCGGATGGAATCGGCCGGGTTCGACCCGGTTCACATGGGCTCGAACGCGTTCAAGCAGGCCAGTATGCTTCTGGCCCTTTACGCCGGCGGCGAAGGGTACCGGGTGGAGGAGAATAACGGGTGTCTGATGCTGGGTTGGCATACTCGGCCGCTGATCGCCACCTCGGCCTGGCAGCTCGCCGCCGATGATTCTTGGCGAGTCGACTCAGTGGTTCGAGCGAGTGAATGA

Protein sequence

MKMKMKREHEDGRSTGGGGGSGSGSGSVYGGGVVKAECSSMSSGKMKMWEEEDGGGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWVQNMLSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPPKHSSSSAGTETNSRKRLKTSTSSIGASSSPPFVATAGIVSEPSRPVVVVDSQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYRIYSPQDGLYSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGWHTRPLIATSAWQLAADDSWRVDSVVRASE
Homology
BLAST of MS027541 vs. NCBI nr
Match: XP_022961073.1 (DELLA protein GAI-like [Cucurbita moschata] >XP_022961085.1 DELLA protein GAI-like [Cucurbita moschata])

HSP 1 Score: 907.5 bits (2344), Expect = 6.1e-260
Identity = 484/622 (77.81%), Postives = 523/622 (84.08%), Query Frame = 0

Query: 1   MKMKMKREHEDGRSTGGGGGSGSGSGSVYGG---GVVKAECSSMSSGKMKMWEEED---G 60
           MKMKMKRE ED RSTGGG         VYGG   GV K ECSS+S GK+KMWEEE+   G
Sbjct: 5   MKMKMKRELEDDRSTGGG---------VYGGGRQGVGKGECSSVSGGKVKMWEEEEQEVG 64

Query: 61  GGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGW 120
           GGGMDELLAVLGYKVRASDMADVAQK+EQLEMVMG+ +EDGISHL+ DTVHYNPSDLS W
Sbjct: 65  GGGMDELLAVLGYKVRASDMADVAQKMEQLEMVMGSVKEDGISHLASDTVHYNPSDLSAW 124

Query: 121 VQNMLSEFNNFPSDLQHRD------PLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGV 180
           +QNMLSEFNN P+            P     SN HS+IQS PSR Y+DDSEYDLSAIPGV
Sbjct: 125 IQNMLSEFNNTPNHFNSSSTSTTPPPQSSQYSNPHSRIQSRPSRLYDDDSEYDLSAIPGV 184

Query: 181 AALPPKHSSSSAGTETNSRKRLK-TSTSSIG-ASSSPPFV---ATAGIVSEPSRPVVVVD 240
           A  P K SS+    ETNSRKR+K    SS+     SPPFV   A AGIVSEPSRPVVVV+
Sbjct: 185 AVFPSKDSSTE--IETNSRKRVKIEGESSVNLLPPSPPFVAAAAAAGIVSEPSRPVVVVE 244

Query: 241 --SQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALAR 300
             SQ+AGIQLVHTLMACAEAVQQENM + +ALVKHIGLL  SQAGAMRKVATYFAEALAR
Sbjct: 245 EGSQDAGIQLVHTLMACAEAVQQENMKIAEALVKHIGLLAVSQAGAMRKVATYFAEALAR 304

Query: 301 RIYRIYSPQDGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLN 360
           RIYRIYSPQDG    YSD+LQ HFYETCPYLKFAHFTANQAILEAFATA+RVHVIDFSLN
Sbjct: 305 RIYRIYSPQDGFYSSYSDILQMHFYETCPYLKFAHFTANQAILEAFATASRVHVIDFSLN 364

Query: 361 QGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFN 420
           QGMQWP LMQALALR GGPP FRLTGIGPP   N+TDSL+QVGWKL++ A AIGV+F+FN
Sbjct: 365 QGMQWPALMQALALRPGGPPTFRLTGIGPPKQGNTTDSLQQVGWKLAQMAGAIGVEFEFN 424

Query: 421 HIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTI 480
            IVCSNL +L+PAAL+I PA VEAVAVNSVFDLHRLLARPGAIEKVLGSIK  KPKIVTI
Sbjct: 425 QIVCSNLANLNPAALEIRPAAVEAVAVNSVFDLHRLLARPGAIEKVLGSIKATKPKIVTI 484

Query: 481 VEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVA 540
           VEQEANHNG VF+DRFTEALHYYSN+FDSLE  S GFEPA+EDV++SE YLGRQICNVVA
Sbjct: 485 VEQEANHNGRVFMDRFTEALHYYSNMFDSLEVSSTGFEPASEDVLLSEVYLGRQICNVVA 544

Query: 541 CEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCL 600
           CEG DRVERHE+L  WRTRMESAGF+PVH+GSNAFKQASMLLAL+AGGEGYRVEENNGCL
Sbjct: 545 CEGPDRVERHESLTHWRTRMESAGFNPVHLGSNAFKQASMLLALFAGGEGYRVEENNGCL 604

BLAST of MS027541 vs. NCBI nr
Match: XP_023550819.1 (DELLA protein GAI-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904.8 bits (2337), Expect = 4.0e-259
Identity = 483/616 (78.41%), Postives = 514/616 (83.44%), Query Frame = 0

Query: 5   MKREHEDGRSTGGGGGSGSGSGSVYGGGVVKAECSSMSSGKMKMWEEED---------GG 64
           MKRE E+ RST   G             VVK ECSS+S GK+KMWEEE+         GG
Sbjct: 1   MKRELEEDRSTADAGRR-----------VVKGECSSLSGGKLKMWEEEEEEEQDANGGGG 60

Query: 65  GGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWV 124
           GGMDELLAVLGYKVRASDMADVA K+EQLEMVMGTAQEDGISHL+ DTVHYNPSDLS WV
Sbjct: 61  GGMDELLAVLGYKVRASDMADVAMKMEQLEMVMGTAQEDGISHLASDTVHYNPSDLSAWV 120

Query: 125 QNMLSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPPKH 184
           QNMLSEFNN  +     +P     S+S  +IQS+ S  Y+DDSEYDLSAIPGVA LPPK 
Sbjct: 121 QNMLSEFNNSTNP----NPQSSLYSDSLHRIQSTTSCLYDDDSEYDLSAIPGVALLPPK- 180

Query: 185 SSSSAGTETNSRKRLKTSTSSIG------ASSSPPFVATAGIVSEPSRPVVVV--DSQEA 244
             SSA TET+SRKRLK    S G      +SSSPPFV TAGIVSEPSRPVVVV  DSQE 
Sbjct: 181 -DSSAETETHSRKRLKIGGESSGHLSPSPSSSSPPFVTTAGIVSEPSRPVVVVGEDSQET 240

Query: 245 GIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYRIY 304
           GIQLVH L+ACAEAVQQENM L DALVKHIG L ASQAGAMRKVATYFA+ALARRIY IY
Sbjct: 241 GIQLVHALLACAEAVQQENMKLADALVKHIGFLAASQAGAMRKVATYFAQALARRIYGIY 300

Query: 305 SPQDGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQWP 364
           SPQDGL   YSD LQ HFYETCPYLKFAHFTANQAILEAFATA RVHVIDFSLNQGMQWP
Sbjct: 301 SPQDGLYSSYSDPLQMHFYETCPYLKFAHFTANQAILEAFATAARVHVIDFSLNQGMQWP 360

Query: 365 VLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVCSN 424
            LMQALALR GGPP FRLTGI PP  DN+ DSL+QVGWKL++ AEAIGV+F+FNHIVCSN
Sbjct: 361 ALMQALALRPGGPPAFRLTGISPPQPDNTADSLQQVGWKLAQMAEAIGVEFEFNHIVCSN 420

Query: 425 LEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQEAN 484
           LEDLDPAAL I P  VEAVAVNSVFDLHRLLARPGAIEKVLG IK  +PKIVTIVEQEAN
Sbjct: 421 LEDLDPAALKIRPTAVEAVAVNSVFDLHRLLARPGAIEKVLGLIKTTQPKIVTIVEQEAN 480

Query: 485 HNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEGTDR 544
           HNGP+F+DRFTEALHYYSNLFDSLEG S GFEPANEDV++SE YLGRQICNVVACEGTDR
Sbjct: 481 HNGPIFLDRFTEALHYYSNLFDSLEGSSIGFEPANEDVLLSEVYLGRQICNVVACEGTDR 540

Query: 545 VERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGWHT 601
           VERHE+L QWRTR+ESAGFDPVH+GSNAFKQASMLLAL+AGGEGYRVEENNGCLMLGWHT
Sbjct: 541 VERHESLTQWRTRIESAGFDPVHLGSNAFKQASMLLALFAGGEGYRVEENNGCLMLGWHT 599

BLAST of MS027541 vs. NCBI nr
Match: XP_023546027.1 (DELLA protein GAI-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 903.7 bits (2334), Expect = 8.9e-259
Identity = 482/622 (77.49%), Postives = 523/622 (84.08%), Query Frame = 0

Query: 1   MKMKMKREHEDGRSTGGGGGSGSGSGSVYGG---GVVKAECSSMSSGKMKMWEEED---- 60
           MKMKMKRE ED RSTGGG         VYGG   GV K ECSS+ SGK+KMWEEE+    
Sbjct: 1   MKMKMKRELEDDRSTGGG---------VYGGGRQGVGKGECSSVLSGKVKMWEEEEEQEV 60

Query: 61  GGGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSG 120
           GGGGMDELLAVLGYKVRASDMADVAQK+EQLEMVMG+ +EDGISHL+ DTVHYNPSDLS 
Sbjct: 61  GGGGMDELLAVLGYKVRASDMADVAQKMEQLEMVMGSVKEDGISHLASDTVHYNPSDLSA 120

Query: 121 WVQNMLSEFNNFP-----SDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGV 180
           W+QNMLSEFNN P     S +    P     SN HS+IQS PSR Y+DDSEYDLSAIPGV
Sbjct: 121 WIQNMLSEFNNTPNHFNSSSISTPPPQSSQYSNPHSRIQSRPSRLYDDDSEYDLSAIPGV 180

Query: 181 AALPPKHSSSSAGTETNSRKRLK-TSTSSIG-ASSSPPFV---ATAGIVSEPSRPVVVVD 240
           A  P K SS+    ETNSRKR+K    SS+     SPPFV   A AGIVSEPSRPVVVV+
Sbjct: 181 AVFPSKDSSTE--IETNSRKRVKIEGESSVNLLPPSPPFVAAAAAAGIVSEPSRPVVVVE 240

Query: 241 --SQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALAR 300
             SQ+AGIQLVHTLMACAEAVQQENM + +ALVKHIGLL  SQAGAMRKVATYFAEALAR
Sbjct: 241 EGSQDAGIQLVHTLMACAEAVQQENMKIAEALVKHIGLLAVSQAGAMRKVATYFAEALAR 300

Query: 301 RIYRIYSPQDGLYS---DLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLN 360
           RIYRIYSPQDG YS   D+LQ HFYETCPYLKFAHFTANQAILEAFA+A+RVHVIDFSLN
Sbjct: 301 RIYRIYSPQDGFYSSYADILQMHFYETCPYLKFAHFTANQAILEAFASASRVHVIDFSLN 360

Query: 361 QGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFN 420
           QGMQWP LMQALALR GGPP FRLTGIGPP   N+ DSL+QVGWKL++ AEAIGV+F+FN
Sbjct: 361 QGMQWPALMQALALRPGGPPTFRLTGIGPPKQGNTADSLQQVGWKLAQMAEAIGVEFEFN 420

Query: 421 HIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTI 480
            IVCSNL +L+PAAL+I P  VEAVAVNSVFDLHRLLARPGAIEKVLGSIK  KPKIVTI
Sbjct: 421 QIVCSNLANLNPAALEIRPPAVEAVAVNSVFDLHRLLARPGAIEKVLGSIKATKPKIVTI 480

Query: 481 VEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVA 540
           VEQEANHNG VF+DRFTEALHYYSN+FDSLE  S GFEPA+EDV++SE YLGRQICNVVA
Sbjct: 481 VEQEANHNGRVFMDRFTEALHYYSNMFDSLEVSSTGFEPASEDVLLSEVYLGRQICNVVA 540

Query: 541 CEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCL 600
           CEG DRVERHE+L  WRTRMESAGF+PVH+GSNAFKQASMLLAL+AGGEGYRVEENNGCL
Sbjct: 541 CEGPDRVERHESLTHWRTRMESAGFNPVHLGSNAFKQASMLLALFAGGEGYRVEENNGCL 600

BLAST of MS027541 vs. NCBI nr
Match: KAG6602029.1 (DELLA protein GAI, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 901.7 bits (2329), Expect = 3.4e-258
Identity = 481/624 (77.08%), Postives = 522/624 (83.65%), Query Frame = 0

Query: 1    MKMKMKREHEDGRSTGGGGGSGSGSGSVYGG---GVVKAECSSMSSGKMKMWEEED---G 60
            MKMKMKRE ED RSTGGG         VYGG   GV K ECSS+S GK+KMW+EE+   G
Sbjct: 542  MKMKMKRELEDDRSTGGG---------VYGGGRQGVGKGECSSVSGGKVKMWKEEEQEVG 601

Query: 61   GGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGW 120
            GGGMDELLAVLGYKVRASDMADVAQK+EQLEMVMG+ +EDGISHL+ DTVHYNPSDLS W
Sbjct: 602  GGGMDELLAVLGYKVRASDMADVAQKMEQLEMVMGSVKEDGISHLASDTVHYNPSDLSAW 661

Query: 121  VQNMLSEFNNFPSDLQHRD--------PLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIP 180
            +QNMLSEF+N P+              P     SN HS+IQS PSR Y+DDSEYDLSAIP
Sbjct: 662  IQNMLSEFSNTPNHFNSSSTSTTPPPPPQSSQYSNPHSRIQSRPSRLYDDDSEYDLSAIP 721

Query: 181  GVAALPPKHSSSSAGTETNSRKRLK-TSTSSIG-ASSSPPFV---ATAGIVSEPSRPVVV 240
            GVA  P K SS+    ETNSRKR+K    SS+     SPPFV   A AGI SEPSRPVVV
Sbjct: 722  GVAVFPSKDSSTE--IETNSRKRVKIEGESSVNLLPPSPPFVAAAAAAGIASEPSRPVVV 781

Query: 241  VD--SQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEAL 300
            V+  SQ+AGIQLVHTLMACAEAVQQENM + +ALVKHIGLL  SQAGAMRKVATYFAEAL
Sbjct: 782  VEEGSQDAGIQLVHTLMACAEAVQQENMKIAEALVKHIGLLAVSQAGAMRKVATYFAEAL 841

Query: 301  ARRIYRIYSPQDGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFS 360
            ARRIYRIYSPQDG    YSD+LQ HFYETCPYLKFAHFTANQAILEAFATA+RVHVIDFS
Sbjct: 842  ARRIYRIYSPQDGFYSSYSDILQMHFYETCPYLKFAHFTANQAILEAFATASRVHVIDFS 901

Query: 361  LNQGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFK 420
            LNQGMQWP LMQALALR GGPP FRLTGIGPP   N+TDSL+QVGWKL++ A AIGV+F+
Sbjct: 902  LNQGMQWPALMQALALRPGGPPTFRLTGIGPPKQGNTTDSLQQVGWKLAQMAGAIGVEFE 961

Query: 421  FNHIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIV 480
            FN IVCSNL +L+PAAL+I PA VEAVAVNSVFDLHRLLARPGAIEKVLGSIK  KPKIV
Sbjct: 962  FNQIVCSNLANLNPAALEIRPAAVEAVAVNSVFDLHRLLARPGAIEKVLGSIKATKPKIV 1021

Query: 481  TIVEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNV 540
            TIVEQEANHNG VF+DRFTEALHYYSN+FDSLE  S GFEPA+EDV++SE YLGRQICNV
Sbjct: 1022 TIVEQEANHNGRVFMDRFTEALHYYSNMFDSLEVSSTGFEPASEDVLLSEVYLGRQICNV 1081

Query: 541  VACEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNG 600
            VACEG DRVERHE+L  WRTRMESAGF+PVH+GSNAFKQASMLLAL+AGGEGYRVEENNG
Sbjct: 1082 VACEGPDRVERHESLTHWRTRMESAGFNPVHLGSNAFKQASMLLALFAGGEGYRVEENNG 1141

BLAST of MS027541 vs. NCBI nr
Match: KAG6579033.1 (DELLA protein GAI, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 901.4 bits (2328), Expect = 4.4e-258
Identity = 481/614 (78.34%), Postives = 514/614 (83.71%), Query Frame = 0

Query: 5   MKREHEDGRSTGGGGGSGSGSGSVYGGGVVKAECSSMSSGKMKMWEEED-------GGGG 64
           MKRE E+ RST   G             VVK ECSS+S GK+KMWEEE+       GGGG
Sbjct: 1   MKRELEEDRSTADAGRR-----------VVKGECSSVSGGKLKMWEEEEEQDANGGGGGG 60

Query: 65  MDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWVQN 124
           MDELLAVLGYKVRASDMADVA K+EQLEMVMGTAQEDGISHL+ DTVHYNPSDLS WVQN
Sbjct: 61  MDELLAVLGYKVRASDMADVAMKMEQLEMVMGTAQEDGISHLASDTVHYNPSDLSAWVQN 120

Query: 125 MLSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPPKHSS 184
           MLSEFNN  +     +P     S+S  +IQS+ S  Y+DDSEYDLSAIPGVA LPPK   
Sbjct: 121 MLSEFNNSTNP----NPQSSLYSDSLHRIQSTTSCLYDDDSEYDLSAIPGVALLPPK--D 180

Query: 185 SSAGTETNSRKRLKTSTSSIG------ASSSPPFVATAGIVSEPSRPVVVV--DSQEAGI 244
           SSA TET+SRKRLK    S G      +SSSPPFV TAGIVSEPSRPVVVV  DSQE GI
Sbjct: 181 SSAETETHSRKRLKIGGESSGHLSSSPSSSSPPFVTTAGIVSEPSRPVVVVGEDSQETGI 240

Query: 245 QLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYRIYSP 304
           QLVH L+ACAEAVQQENM L DALVKHIG L ASQAGAMRKVATYFA+ALARRIY IYSP
Sbjct: 241 QLVHALLACAEAVQQENMKLADALVKHIGFLAASQAGAMRKVATYFAQALARRIYGIYSP 300

Query: 305 QDGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQWPVL 364
           QDGL   YSD LQ HFYETCPYLKFAHFTANQAILEAFATA RVHVIDFSLNQGMQWP L
Sbjct: 301 QDGLYSSYSDPLQMHFYETCPYLKFAHFTANQAILEAFATAARVHVIDFSLNQGMQWPAL 360

Query: 365 MQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVCSNLE 424
           MQALALR GGPP FRLTGI PP  DN+ DSL+QVGWKL++ AEAIGV+F+FNHIVCSNLE
Sbjct: 361 MQALALRPGGPPAFRLTGISPPQPDNTADSLQQVGWKLAQMAEAIGVEFEFNHIVCSNLE 420

Query: 425 DLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQEANHN 484
           DLDPAAL I P  VEAVAVNSVFDLHRLLARPGAIEKVLG IK  +PKIVTIVEQEANHN
Sbjct: 421 DLDPAALKIRPTAVEAVAVNSVFDLHRLLARPGAIEKVLGLIKTTQPKIVTIVEQEANHN 480

Query: 485 GPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEGTDRVE 544
           GP+F+DRFTEALHYYSNLFDSLEG S GFEPANEDV++SE YLGRQICNVVACEGT+RVE
Sbjct: 481 GPIFLDRFTEALHYYSNLFDSLEGSSIGFEPANEDVLLSEVYLGRQICNVVACEGTNRVE 540

Query: 545 RHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGWHTRP 601
           RHE+L QWRTR+ESAGFDPVH+GSNAFKQASMLLAL+AGGEGYRVEENNGCLMLGWHTRP
Sbjct: 541 RHESLTQWRTRIESAGFDPVHLGSNAFKQASMLLALFAGGEGYRVEENNGCLMLGWHTRP 597

BLAST of MS027541 vs. ExPASy Swiss-Prot
Match: Q84TQ7 (DELLA protein GAI OS=Gossypium hirsutum OX=3635 GN=GAI PE=2 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 6.3e-207
Identity = 394/588 (67.01%), Postives = 448/588 (76.19%), Query Frame = 0

Query: 5   MKREHEDGRSTGGGGGSGSGSGSVYGGGVVKAECSSMSSGKMKMWEEEDGGGGM-DELLA 64
           MKR+H++         SGSGS          AE SS+   K K+WEE+   GGM DELLA
Sbjct: 1   MKRDHQE--------ISGSGSN--------PAESSSI---KGKLWEEDPDAGGMDDELLA 60

Query: 65  VLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWVQNMLSEFN 124
           VLGYKVR+SDMADVAQKLE LE VMGTAQEDGIS L  DTVH+NPSDLSGWVQN+L EFN
Sbjct: 61  VLGYKVRSSDMADVAQKLEMLEKVMGTAQEDGISQLG-DTVHFNPSDLSGWVQNLLIEFN 120

Query: 125 NFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPPKHSSSSAGTE 184
                                   ++P   +NDDSEYDL AIPGVAA PP    S  G E
Sbjct: 121 G---------------------STTTPDPNFNDDSEYDLRAIPGVAAYPP--VKSDPGLE 180

Query: 185 TNSRKRLKTSTSSIGASSSPPFVATAGIVSEPSRPVVVVDSQEAGIQLVHTLMACAEAVQ 244
             +RKR KT +SS  +S++             +RPVV++DSQEAG++LVHTLMACAEAVQ
Sbjct: 181 I-TRKRAKTESSSSSSSTT-------------TRPVVLIDSQEAGVRLVHTLMACAEAVQ 240

Query: 245 QENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYRIYSPQ--DGLYSDLLQNH 304
           Q+N+ L DALVKHIGLL +SQ GAMRKVATYFAEALARRIYRI+ P   D  Y+D LQ  
Sbjct: 241 QDNLKLADALVKHIGLLASSQTGAMRKVATYFAEALARRIYRIFPPDSLDPSYNDKLQIP 300

Query: 305 FYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQWPVLMQALALRVGGPPEFR 364
           FYETCPYLKFAHFTANQAILEAF+ A+RVHVIDF L QGMQWP LMQALALR GGPP FR
Sbjct: 301 FYETCPYLKFAHFTANQAILEAFSMASRVHVIDFGLKQGMQWPALMQALALRPGGPPAFR 360

Query: 365 LTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVCSNLEDLDPAALDILPAEVE 424
           LTGIGPP  DN TD+L+QVGWKL++ AE IG++F+F   V ++L DL+P  LDI P E+E
Sbjct: 361 LTGIGPPQPDN-TDALQQVGWKLAQLAERIGIEFEFRGFVANSLADLEPEMLDIRPPEIE 420

Query: 425 AVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQEANHNGPVFVDRFTEALHYY 484
            VAVN+VF+LH LLARPG IEKV+ SIK MKPKIVT+VEQEANHNGPVF+DRFTEALHYY
Sbjct: 421 VVAVNAVFELHPLLARPGGIEKVVSSIKAMKPKIVTVVEQEANHNGPVFLDRFTEALHYY 480

Query: 485 SNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEGTDRVERHETLAQWRTRMESA 544
           S LFDSLEG  +G  PA++D+ MSE YLGRQICNVVACEG DRVERHE L QWRTRME+A
Sbjct: 481 STLFDSLEG--SGVAPASQDLAMSELYLGRQICNVVACEGMDRVERHEPLTQWRTRMETA 528

Query: 545 GFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGWHTRPLIA 590
           G  PVH+GSNA+KQASMLLAL+A G+GYRVEENNGCLMLGWHTRPLIA
Sbjct: 541 GVSPVHLGSNAYKQASMLLALFASGDGYRVEENNGCLMLGWHTRPLIA 528

BLAST of MS027541 vs. ExPASy Swiss-Prot
Match: Q7Y1B6 (DELLA protein GAI OS=Solanum lycopersicum OX=4081 GN=GAI PE=2 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 6.9e-206
Identity = 384/577 (66.55%), Postives = 444/577 (76.95%), Query Frame = 0

Query: 40  SMSSGKMKMW---EEEDGGGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGI 99
           ++SSGK K+W   EEE    GMDELLAVLGYKV++SDMADVAQKLEQLEM MGT  EDGI
Sbjct: 20  AVSSGKSKIWEEDEEEKPDAGMDELLAVLGYKVKSSDMADVAQKLEQLEMAMGTTMEDGI 79

Query: 100 SHLSCDTVHYNPSDLSGWVQNMLSEFN-NFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYN 159
           +HLS DTVH NPSD++GWVQ+MLS  + NF    Q  D L     +S S I  S +   +
Sbjct: 80  THLSTDTVHKNPSDMAGWVQSMLSSISTNFDMCNQENDVLVSGCGSSSSIIDFSQNHRTS 139

Query: 160 DDSEYDLSAIPGVAALPPKHSSSSAGTETNSRKRLKTSTSSIGASSSPPFVATAGIVSEP 219
             S+ DL AIPG A              ++S KR +++TSS   +SS          S  
Sbjct: 140 TISDDDLRAIPGGAVF-----------NSDSNKRHRSTTSSFSTTSSSMVTD-----SSA 199

Query: 220 SRPVVVVDSQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYF 279
           +RPVV+VDSQE G++LVHTLMACAEAVQQEN+TL D LV+HIG+L  SQ+GAMRKVATYF
Sbjct: 200 TRPVVLVDSQETGVRLVHTLMACAEAVQQENLTLADQLVRHIGILAVSQSGAMRKVATYF 259

Query: 280 AEALARRIYRIYSPQDGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHV 339
           AEALARRIY+IY PQD +   Y+D+LQ HFYETCPYLKFAHFTANQAILEAF   N+VHV
Sbjct: 260 AEALARRIYKIY-PQDSMESSYTDVLQMHFYETCPYLKFAHFTANQAILEAFTGCNKVHV 319

Query: 340 IDFSLNQGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIG 399
           IDFSL QGMQWP LMQALALR GGPP FRLTGIGPP  DN TD+L+QVGWKL++ AE IG
Sbjct: 320 IDFSLKQGMQWPALMQALALRPGGPPAFRLTGIGPPQPDN-TDALQQVGWKLAQLAETIG 379

Query: 400 VKFKFNHIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMK 459
           V+F+F   V ++L DLD   LDI P+E EAVA+NSVF+LHRLL+RPGAIEKVL SIK++ 
Sbjct: 380 VEFEFRGFVANSLADLDATILDIRPSETEAVAINSVFELHRLLSRPGAIEKVLNSIKQIN 439

Query: 460 PKIVTIVEQEANHNGPVFVDRFTEALHYYSNLFDSLE--GYSNGFEPA---------NED 519
           PKIVT+VEQEANHN  VF+DRF EALHYYS +FDSLE  G S+   P          N+D
Sbjct: 440 PKIVTLVEQEANHNAGVFIDRFNEALHYYSTMFDSLESSGSSSSASPTGILPQPPVNNQD 499

Query: 520 VVMSEFYLGRQICNVVACEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLA 579
           +VMSE YLGRQICNVVACEG+DRVERHETL QWR RM S+GFDPVH+GSNAFKQASMLLA
Sbjct: 500 LVMSEVYLGRQICNVVACEGSDRVERHETLNQWRVRMNSSGFDPVHLGSNAFKQASMLLA 559

Query: 580 LYAGGEGYRVEENNGCLMLGWHTRPLIATSAWQLAAD 599
           L+AGG+GYRVEEN+GCLMLGWHTRPLIATSAW+L  D
Sbjct: 560 LFAGGDGYRVEENDGCLMLGWHTRPLIATSAWKLLPD 578

BLAST of MS027541 vs. ExPASy Swiss-Prot
Match: A0A396IUP1 (DELLA protein 1 OS=Medicago truncatula OX=3880 GN=DELLA1 PE=1 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 5.5e-203
Identity = 389/617 (63.05%), Postives = 469/617 (76.01%), Query Frame = 0

Query: 5   MKREHEDGRSTGGGGGSGSGSGSV----YGGGVVKAECSSM--SSGKMKMW--EEEDGGG 64
           MKREH++  S GGG  S +   +         +   ECSSM  ++ K  MW  E+E  GG
Sbjct: 1   MKREHQE--SFGGGVISNNNKTNTNHLNSSKNINFGECSSMQNTNTKQNMWREEKETNGG 60

Query: 65  GMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWVQ 124
           GMDELLA LGYKVR+SDMADVAQKLEQLEMVMG+AQE+GI+HLS DTVHY+P+DL  WVQ
Sbjct: 61  GMDELLAALGYKVRSSDMADVAQKLEQLEMVMGSAQEEGINHLSSDTVHYDPTDLYSWVQ 120

Query: 125 NMLSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPPKHS 184
            ML+E N  P   Q  DPL   +  S S+I ++    +NDDSEYDLSAIPG+AA PP+  
Sbjct: 121 TMLTELN--PDSSQINDPLA--SLGSSSEILNNT---FNDDSEYDLSAIPGMAAYPPQEE 180

Query: 185 SSSAGTETNSRKRLKTSTSSIGASSSPPFVATAGIVSEPSRPVVVVDSQEAGIQLVHTLM 244
           +++A       KR+KT +      S P  V +     E +RPVV+VD+QE G++LVHTLM
Sbjct: 181 NTAA-------KRMKTWSE---PESEPAVVMSPPPAVENTRPVVLVDTQETGVRLVHTLM 240

Query: 245 ACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYRIYSPQ---DGL 304
           ACAEA+QQ+N+ L +ALVKHI LL + Q GAMRKVA+YFA+ALARRIY   +P+   D  
Sbjct: 241 ACAEAIQQKNLKLAEALVKHISLLASLQTGAMRKVASYFAQALARRIYG--NPEETIDSS 300

Query: 305 YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQWPVLMQALALR 364
           +S++L  HFYE+ PYLKFAHFTANQAILEAFA A RVHVIDF L QGMQWP LMQALALR
Sbjct: 301 FSEILHMHFYESSPYLKFAHFTANQAILEAFAGAGRVHVIDFGLKQGMQWPALMQALALR 360

Query: 365 VGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVCSNLEDLDPAAL 424
            GGPP FRLTGIGPP +DN TD+L+QVGWKL++ A+ IGV+F+F   VC+++ DLDP  L
Sbjct: 361 PGGPPTFRLTGIGPPQADN-TDALQQVGWKLAQLAQTIGVQFEFRGFVCNSIADLDPNML 420

Query: 425 DILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQEANHNGPVFVDR 484
           +I P   EAVAVNSVF+LH +LARPG++EKVL ++K++ PKIVTIVEQEANHNGPVFVDR
Sbjct: 421 EIRPG--EAVAVNSVFELHTMLARPGSVEKVLNTVKKINPKIVTIVEQEANHNGPVFVDR 480

Query: 485 FTEALHYYSNLFDSLEG----------YSNGFEPANEDVVMSEFYLGRQICNVVACEGTD 544
           FTEALHYYS+LFDSLEG           S G    ++D++MSE YLG+QICNVVA EG D
Sbjct: 481 FTEALHYYSSLFDSLEGSNSSSNNSNSNSTGLGSPSQDLLMSEIYLGKQICNVVAYEGVD 540

Query: 545 RVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGWH 601
           RVERHETL QWR+RM SAGF+PVH+GSNAFKQAS LLAL+AGG+GYRVEENNGCLMLGWH
Sbjct: 541 RVERHETLTQWRSRMGSAGFEPVHLGSNAFKQASTLLALFAGGDGYRVEENNGCLMLGWH 593

BLAST of MS027541 vs. ExPASy Swiss-Prot
Match: Q8S4W7 (DELLA protein GAI1 OS=Vitis vinifera OX=29760 GN=GAI1 PE=2 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 2.8e-199
Identity = 374/571 (65.50%), Postives = 438/571 (76.71%), Query Frame = 0

Query: 38  CSSMSSGKMKMWE-EEDGGGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGI 97
           CS+  +GK KMW+ +     GMDELLAVLGY V+ASDMA+VAQKLEQLE V+  AQEDG+
Sbjct: 13  CSTSPTGKGKMWDADPQQDAGMDELLAVLGYNVKASDMAEVAQKLEQLEEVIVNAQEDGL 72

Query: 98  SHLSCDTVHYNPSDLSGWVQNMLSEFNNFPS---DLQHRDPLC-LHNSNSHSQIQSSPSR 157
           SHL+ +TVHYNPSDLS W+ +MLSEFN  P+   D     P+  L  +N  +Q +  PS 
Sbjct: 73  SHLASETVHYNPSDLSNWLGSMLSEFNPTPNCALDNPFLPPISPLDYTNCSTQPKQEPSI 132

Query: 158 FYNDDSEYDLSAIPGVAAL----PPKHSSSSAGTETNSRKRLKTSTSSIGASSSPPFVAT 217
           F +   +YDL AIPG A       P     +        KRLK +TS+  A+S    +  
Sbjct: 133 FDSPSLDYDLKAIPGKALYSHIEQPPQQPPAPPLYQRDNKRLKPTTSAT-ANSVSSVIGG 192

Query: 218 AGIVSEPSRPVVVVDSQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAM 277
            G+ +E +RPVV+VDSQE GI+LVHTLMACAEAVQQEN+ L +ALVK IG L  SQAGAM
Sbjct: 193 WGVPTESARPVVLVDSQETGIRLVHTLMACAEAVQQENLKLAEALVKQIGFLAVSQAGAM 252

Query: 278 RKVATYFAEALARRIYRIY--SPQDGLYSDLLQNHFYETCPYLKFAHFTANQAILEAFAT 337
           RKVATYFAE LARRIYR+Y   P D  +SD+LQ HFYETCPYLKFAHFTANQAILEAF  
Sbjct: 253 RKVATYFAEGLARRIYRLYPDKPLDSSFSDILQMHFYETCPYLKFAHFTANQAILEAFEG 312

Query: 338 ANRVHVIDFSLNQGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSR 397
             RVHVIDFS+ QGMQWP LMQALALR GGPP FRLTGIGPP +DN TD L +VGWKL++
Sbjct: 313 KKRVHVIDFSMKQGMQWPALMQALALRPGGPPSFRLTGIGPPSTDN-TDHLHEVGWKLAQ 372

Query: 398 TAEAIGVKFKFNHIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLG 457
            AE I V+F++   V ++L DLD + L++   + E+VAVNSVF+LH LLARPG IE+VL 
Sbjct: 373 LAETIHVEFEYRGFVANSLADLDASMLEL--RDGESVAVNSVFELHSLLARPGGIERVLS 432

Query: 458 SIKRMKPKIVTIVEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPAN-EDVVMS 517
           ++K MKP IVTIVEQEANHNGPVF+DRFTE+LHYYS LFDSLEG   G  P N +D +MS
Sbjct: 433 AVKDMKPDIVTIVEQEANHNGPVFLDRFTESLHYYSTLFDSLEGC--GVSPVNTQDKLMS 492

Query: 518 EFYLGRQICNVVACEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAG 577
           E YLG+QICNVVACEG +RVERHETLAQWR R+ SAGFDPV++GSNAFKQASMLLAL+AG
Sbjct: 493 EVYLGQQICNVVACEGPERVERHETLAQWRARLGSAGFDPVNLGSNAFKQASMLLALFAG 552

Query: 578 GEGYRVEENNGCLMLGWHTRPLIATSAWQLA 597
           G+GYRVEENNGCLMLGWHTRPLIATSAWQLA
Sbjct: 553 GDGYRVEENNGCLMLGWHTRPLIATSAWQLA 577

BLAST of MS027541 vs. ExPASy Swiss-Prot
Match: Q5BN22 (DELLA protein RGA2 OS=Brassica campestris OX=3711 GN=RGA2 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 7.0e-190
Identity = 352/580 (60.69%), Postives = 431/580 (74.31%), Query Frame = 0

Query: 36  AECSSMSSGKMKMWEEEDGGGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDG 95
           A   S S  KM M +EE+ GG MDELLAVLGYKVR+S+MA+VA KLEQLE +MG  QEDG
Sbjct: 22  ANTGSSSKDKMMMVKEEEDGGNMDELLAVLGYKVRSSEMAEVALKLEQLETMMGNVQEDG 81

Query: 96  ISHLSCDTVHYNPSDLSGWVQNMLSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYN 155
           +S+L+ DTVHYNPS+L  W+ NML+EFN  P ++            ++S +  +      
Sbjct: 82  LSNLATDTVHYNPSELYSWLDNMLTEFNPPPPEI------------NNSFLAGA------ 141

Query: 156 DDSEYDLSAIPGVA--------ALPPKHSSSSAGTETNSRKRLKTS-------TSSIGAS 215
             S+YDL AIPG A        A+    SS+ AG  + S KRLK+        T +   +
Sbjct: 142 GGSDYDLKAIPGNAIYARSDQFAIDSSSSSNQAGDNSQSTKRLKSCSSPDSLVTGTTVTT 201

Query: 216 SSPPFVATAGIVSEPSRPVVVVDSQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLL 275
           ++     + G+ +E +R +V+VDSQE G++LVH LMACAEA+Q  ++++ +ALVK IG L
Sbjct: 202 TTTESTRSVGLAAESTRSMVLVDSQENGVRLVHALMACAEAIQNNDLSIAEALVKQIGFL 261

Query: 276 MASQAGAMRKVATYFAEALARRIYRIYSPQ---DGLYSDLLQNHFYETCPYLKFAHFTAN 335
             SQAGAMRKVATYFAEALARRIYR+  PQ   D   SD LQ HFYETCPYLKFAHFTAN
Sbjct: 262 AVSQAGAMRKVATYFAEALARRIYRLSPPQTQIDHSLSDTLQMHFYETCPYLKFAHFTAN 321

Query: 336 QAILEAFATANRVHVIDFSLNQGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSL 395
           QAILEAF    RVHVIDFS+NQG+QWP LMQALALR GGPP FRLTGIGPP +DNS D L
Sbjct: 322 QAILEAFEGKKRVHVIDFSMNQGLQWPALMQALALREGGPPVFRLTGIGPPAADNS-DHL 381

Query: 396 EQVGWKLSRTAEAIGVKFKFNHIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLAR 455
            +VG KL++ AEAI V+F++   V ++L DLD + L++ P+E+EAVAVNSVF+LH+LL R
Sbjct: 382 HEVGCKLAQLAEAIHVEFEYRGFVANSLADLDASMLELRPSEIEAVAVNSVFELHKLLGR 441

Query: 456 PGAIEKVLGSIKRMKPKIVTIVEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEP 515
            G IEKVLG +K++KP I T+VEQE++HNGPVF+DRFTE+LHYYS LFDSLEG      P
Sbjct: 442 TGGIEKVLGVVKQIKPVIFTVVEQESSHNGPVFLDRFTESLHYYSTLFDSLEGV-----P 501

Query: 516 ANEDVVMSEFYLGRQICNVVACEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQAS 575
           +++D VMSE YLG+QICN+VACEG DRVERHETL+QW  R  S+GF P H+GSNAFKQAS
Sbjct: 502 SSQDKVMSEVYLGKQICNLVACEGPDRVERHETLSQWANRFGSSGFAPAHLGSNAFKQAS 561

Query: 576 MLLALYAGGEGYRVEENNGCLMLGWHTRPLIATSAWQLAA 598
           MLLAL+ GGEGYRVEENNGCLMLGWHTRPLI TSAW+L+A
Sbjct: 562 MLLALFNGGEGYRVEENNGCLMLGWHTRPLITTSAWKLSA 577

BLAST of MS027541 vs. ExPASy TrEMBL
Match: A0A6J1HAU0 (DELLA protein OS=Cucurbita moschata OX=3662 GN=LOC111461688 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 3.0e-260
Identity = 484/622 (77.81%), Postives = 523/622 (84.08%), Query Frame = 0

Query: 1   MKMKMKREHEDGRSTGGGGGSGSGSGSVYGG---GVVKAECSSMSSGKMKMWEEED---G 60
           MKMKMKRE ED RSTGGG         VYGG   GV K ECSS+S GK+KMWEEE+   G
Sbjct: 5   MKMKMKRELEDDRSTGGG---------VYGGGRQGVGKGECSSVSGGKVKMWEEEEQEVG 64

Query: 61  GGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGW 120
           GGGMDELLAVLGYKVRASDMADVAQK+EQLEMVMG+ +EDGISHL+ DTVHYNPSDLS W
Sbjct: 65  GGGMDELLAVLGYKVRASDMADVAQKMEQLEMVMGSVKEDGISHLASDTVHYNPSDLSAW 124

Query: 121 VQNMLSEFNNFPSDLQHRD------PLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGV 180
           +QNMLSEFNN P+            P     SN HS+IQS PSR Y+DDSEYDLSAIPGV
Sbjct: 125 IQNMLSEFNNTPNHFNSSSTSTTPPPQSSQYSNPHSRIQSRPSRLYDDDSEYDLSAIPGV 184

Query: 181 AALPPKHSSSSAGTETNSRKRLK-TSTSSIG-ASSSPPFV---ATAGIVSEPSRPVVVVD 240
           A  P K SS+    ETNSRKR+K    SS+     SPPFV   A AGIVSEPSRPVVVV+
Sbjct: 185 AVFPSKDSSTE--IETNSRKRVKIEGESSVNLLPPSPPFVAAAAAAGIVSEPSRPVVVVE 244

Query: 241 --SQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALAR 300
             SQ+AGIQLVHTLMACAEAVQQENM + +ALVKHIGLL  SQAGAMRKVATYFAEALAR
Sbjct: 245 EGSQDAGIQLVHTLMACAEAVQQENMKIAEALVKHIGLLAVSQAGAMRKVATYFAEALAR 304

Query: 301 RIYRIYSPQDGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLN 360
           RIYRIYSPQDG    YSD+LQ HFYETCPYLKFAHFTANQAILEAFATA+RVHVIDFSLN
Sbjct: 305 RIYRIYSPQDGFYSSYSDILQMHFYETCPYLKFAHFTANQAILEAFATASRVHVIDFSLN 364

Query: 361 QGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFN 420
           QGMQWP LMQALALR GGPP FRLTGIGPP   N+TDSL+QVGWKL++ A AIGV+F+FN
Sbjct: 365 QGMQWPALMQALALRPGGPPTFRLTGIGPPKQGNTTDSLQQVGWKLAQMAGAIGVEFEFN 424

Query: 421 HIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTI 480
            IVCSNL +L+PAAL+I PA VEAVAVNSVFDLHRLLARPGAIEKVLGSIK  KPKIVTI
Sbjct: 425 QIVCSNLANLNPAALEIRPAAVEAVAVNSVFDLHRLLARPGAIEKVLGSIKATKPKIVTI 484

Query: 481 VEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVA 540
           VEQEANHNG VF+DRFTEALHYYSN+FDSLE  S GFEPA+EDV++SE YLGRQICNVVA
Sbjct: 485 VEQEANHNGRVFMDRFTEALHYYSNMFDSLEVSSTGFEPASEDVLLSEVYLGRQICNVVA 544

Query: 541 CEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCL 600
           CEG DRVERHE+L  WRTRMESAGF+PVH+GSNAFKQASMLLAL+AGGEGYRVEENNGCL
Sbjct: 545 CEGPDRVERHESLTHWRTRMESAGFNPVHLGSNAFKQASMLLALFAGGEGYRVEENNGCL 604

BLAST of MS027541 vs. ExPASy TrEMBL
Match: A0A6J1FFR8 (DELLA protein OS=Cucurbita moschata OX=3662 GN=LOC111445074 PE=3 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.6e-258
Identity = 480/613 (78.30%), Postives = 513/613 (83.69%), Query Frame = 0

Query: 5   MKREHEDGRSTGGGGGSGSGSGSVYGGGVVKAECSSMSSGKMKMWEEED------GGGGM 64
           MKRE E+ RST   G             VVK ECSS+S GK+KMWEEE+      GGGGM
Sbjct: 1   MKRELEEDRSTADAGRR-----------VVKGECSSVSGGKLKMWEEEEEQDANGGGGGM 60

Query: 65  DELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWVQNM 124
           DELLAVLGYKVRASDMADVA K+EQLEMVMGTAQEDGISHL+ DTVHYNPSDLS WVQNM
Sbjct: 61  DELLAVLGYKVRASDMADVAMKMEQLEMVMGTAQEDGISHLASDTVHYNPSDLSAWVQNM 120

Query: 125 LSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPPKHSSS 184
           LSEFNN  +     +P     S+S  +IQS+ S  Y+DDSEYDLSAIPGVA LPPK SS+
Sbjct: 121 LSEFNNSTNP----NPQSSLYSDSLHRIQSTTSCLYDDDSEYDLSAIPGVALLPPKDSST 180

Query: 185 SAGTETNSRKRLKTSTSSIG------ASSSPPFVATAGIVSEPSRPVVVV--DSQEAGIQ 244
               ET+SRKRLK    S G      +SSSPPFV TAGIVSEPSRPVVVV  DSQE GIQ
Sbjct: 181 E--IETHSRKRLKIGGESSGHLSPSPSSSSPPFVTTAGIVSEPSRPVVVVGEDSQETGIQ 240

Query: 245 LVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYRIYSPQ 304
           LVH L+ACAEAVQQENM L DALVKHIG L ASQAGAMRKVATYFA+ALARRIY IYSPQ
Sbjct: 241 LVHALLACAEAVQQENMKLADALVKHIGFLAASQAGAMRKVATYFAQALARRIYGIYSPQ 300

Query: 305 DGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQWPVLM 364
           DGL   YSD LQ HFYETCPYLKFAHFTANQAILEAFATA RVHVIDFSLNQGMQWP LM
Sbjct: 301 DGLYSSYSDPLQMHFYETCPYLKFAHFTANQAILEAFATAARVHVIDFSLNQGMQWPALM 360

Query: 365 QALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVCSNLED 424
           QALALR GGPP FRLTGI PP  DN+ DSL+QVGWKL++ AEAIGV+F+FNHIVCSNLED
Sbjct: 361 QALALRPGGPPAFRLTGISPPQPDNTADSLQQVGWKLAQMAEAIGVEFEFNHIVCSNLED 420

Query: 425 LDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQEANHNG 484
           LDPAAL I P  VEAVAVNSVFDLHRLLARPGAIEKVLG IK  +PKIVTIVEQEANHNG
Sbjct: 421 LDPAALKIRPTAVEAVAVNSVFDLHRLLARPGAIEKVLGLIKTTQPKIVTIVEQEANHNG 480

Query: 485 PVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEGTDRVER 544
           P+F+DRFTEALHYYSNLFDSLEG S GFEPANEDV++SE YLGRQICNVVACEGTDRVER
Sbjct: 481 PIFLDRFTEALHYYSNLFDSLEGSSIGFEPANEDVLLSEVYLGRQICNVVACEGTDRVER 540

Query: 545 HETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGWHTRPL 601
           HE+L QWRTR+ESAGFDPVH+GSNAFKQASMLLAL+AGGEGYRVEENNGCLMLGWHTRPL
Sbjct: 541 HESLTQWRTRIESAGFDPVHLGSNAFKQASMLLALFAGGEGYRVEENNGCLMLGWHTRPL 596

BLAST of MS027541 vs. ExPASy TrEMBL
Match: A0A6J1JHZ8 (DELLA protein OS=Cucurbita maxima OX=3661 GN=LOC111487159 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 1.1e-257
Identity = 480/616 (77.92%), Postives = 521/616 (84.58%), Query Frame = 0

Query: 3   MKMKREHEDGRSTGGGGGSGSGSGSVYGG---GVVKAECSSMSSGKMKMWEEEDG--GGG 62
           MKMKRE ED RSTGGG         VYGG   GV K ECSS+ SGK+KMWEEE+   GGG
Sbjct: 1   MKMKRELEDDRSTGGG---------VYGGGRQGVGKGECSSVLSGKVKMWEEEEQEVGGG 60

Query: 63  MDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWVQN 122
           MDELLAVLGYKVRASDMADVAQK+EQLEMVMG+ QEDGISHL+ DTVHYNPSDLS W+QN
Sbjct: 61  MDELLAVLGYKVRASDMADVAQKMEQLEMVMGSVQEDGISHLASDTVHYNPSDLSAWIQN 120

Query: 123 MLSEFNNFP----SDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPP 182
           MLSEFNN P    S      P     SN HS+IQS PSR Y+DDSEYDLSAIPGVA  P 
Sbjct: 121 MLSEFNNTPNHFNSSSTSTPPQSSQYSNPHSRIQSRPSRLYDDDSEYDLSAIPGVAVFPS 180

Query: 183 KHSSSSAGTETNSRKRLK-TSTSSIG-ASSSPPFV--ATAGIVSEPSRPVVVVD--SQEA 242
           K  S+    ETNSRKR+K    +S+     SPPFV  A AGIVSEPSRPVVVV+  SQ+A
Sbjct: 181 KDYSTE--FETNSRKRVKIEGENSVNLLPPSPPFVAAAAAGIVSEPSRPVVVVEEGSQDA 240

Query: 243 GIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYRIY 302
           GIQLVHTLMACAEAVQQENM + +ALVKHIGLL  SQAGAMRKVATYFAEALARRIYRIY
Sbjct: 241 GIQLVHTLMACAEAVQQENMKIAEALVKHIGLLAVSQAGAMRKVATYFAEALARRIYRIY 300

Query: 303 SPQDGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQWP 362
           SPQDG    YSD+LQ HFYETCPYLKFAHFTANQAILEAFATA+RVHVIDFSLNQGMQWP
Sbjct: 301 SPQDGFYSSYSDILQMHFYETCPYLKFAHFTANQAILEAFATASRVHVIDFSLNQGMQWP 360

Query: 363 VLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVCSN 422
            LMQALALR GGPP FRLTGIGPP   N+ DSL+QVGWKL++ AEAIGV+F+FNHIVCSN
Sbjct: 361 ALMQALALRPGGPPTFRLTGIGPPKQGNTADSLQQVGWKLAQMAEAIGVEFEFNHIVCSN 420

Query: 423 LEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQEAN 482
           L +L+PAAL+I  A VEAVAVNSVFDLHRLLARPGAIEKVLGSIK  KPKIVTIVEQEAN
Sbjct: 421 LANLNPAALEIRLASVEAVAVNSVFDLHRLLARPGAIEKVLGSIKATKPKIVTIVEQEAN 480

Query: 483 HNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEGTDR 542
           HNG VF+DRFTEALHYYSN+FDSLE  S GFEPA+EDV++SE YLGRQICNVVACEG+DR
Sbjct: 481 HNGRVFMDRFTEALHYYSNMFDSLEVSSTGFEPASEDVLLSEVYLGRQICNVVACEGSDR 540

Query: 543 VERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGWHT 601
           VERHE+L QWR+RM+SAGF+PVH+GSNAFKQASMLLAL+AGGEGYRVEENNGCLMLGWHT
Sbjct: 541 VERHESLTQWRSRMKSAGFNPVHLGSNAFKQASMLLALFAGGEGYRVEENNGCLMLGWHT 600

BLAST of MS027541 vs. ExPASy TrEMBL
Match: A0A6J1K174 (DELLA protein OS=Cucurbita maxima OX=3661 GN=LOC111489223 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.3e-255
Identity = 477/614 (77.69%), Postives = 509/614 (82.90%), Query Frame = 0

Query: 5   MKREHEDGRSTGGGGGSGSGSGSVYGGGVVKAECSSMSSGKMKMWEEED-------GGGG 64
           MKRE E+ RST   G             VVK ECSS+S GK+KMWEEE+       GGGG
Sbjct: 1   MKRELEEDRSTADAGRR-----------VVKGECSSLSGGKLKMWEEEEEQDANGGGGGG 60

Query: 65  MDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWVQN 124
           MDELLAVLGYKVRASDMADVA K+EQLEMVMGTAQEDGISHL+ DTVHYNPSDLS WVQN
Sbjct: 61  MDELLAVLGYKVRASDMADVALKMEQLEMVMGTAQEDGISHLASDTVHYNPSDLSAWVQN 120

Query: 125 MLSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPPKHSS 184
           MLSEFNN  +     +P     S+S   IQS+ S  Y+DDSEYDLSAIPGVA LPPK SS
Sbjct: 121 MLSEFNNSTNP----NPQSSLYSDSLHGIQSTTSCLYDDDSEYDLSAIPGVALLPPKDSS 180

Query: 185 SSAGTETNSRKRLKTSTSSIG------ASSSPPFVATAGIVSEPSRPVVVV--DSQEAGI 244
           +    ET+ RKRLK    S G      +SSSPPFV TAGIVSEPSR VVVV  DSQE GI
Sbjct: 181 TE--IETHVRKRLKIGGESSGHLSPSPSSSSPPFVTTAGIVSEPSRSVVVVGEDSQETGI 240

Query: 245 QLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYRIYSP 304
           QLVH L+ACAEAVQQENM L DALVKHIG L ASQAGAMRKVATYFA+ALARRIY IYSP
Sbjct: 241 QLVHALLACAEAVQQENMKLADALVKHIGFLAASQAGAMRKVATYFAQALARRIYGIYSP 300

Query: 305 QDGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQWPVL 364
           QDGL   YSD LQ HFYETCPYLKFAHFTANQAILEAFATA RVHVIDFSLNQGMQWP L
Sbjct: 301 QDGLYSSYSDPLQMHFYETCPYLKFAHFTANQAILEAFATAARVHVIDFSLNQGMQWPAL 360

Query: 365 MQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVCSNLE 424
           MQALALR GGPP FRLTGI PP  DN+ DSL+QVGWKL++ AEAIGV+F+FNHIVCSNLE
Sbjct: 361 MQALALRPGGPPAFRLTGISPPQPDNTADSLQQVGWKLAQMAEAIGVEFEFNHIVCSNLE 420

Query: 425 DLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQEANHN 484
           DLDPAAL I P  VEAVAVNSVFDLHRLLARPGAIEKVLG I   +PKIVTIVEQEANHN
Sbjct: 421 DLDPAALKIRPTAVEAVAVNSVFDLHRLLARPGAIEKVLGLINTTQPKIVTIVEQEANHN 480

Query: 485 GPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEGTDRVE 544
           GP+F+DRFTEALHYYSNLFDSLEG S GFEPANEDV++SE YLGRQICNVVACEGTDRVE
Sbjct: 481 GPIFLDRFTEALHYYSNLFDSLEGSSIGFEPANEDVLLSEVYLGRQICNVVACEGTDRVE 540

Query: 545 RHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGWHTRP 601
           RHE+L QWRTR+ES+GFDPVH+GSNAFKQASMLLAL+AGGEGYRVEENNGCLMLGWHTRP
Sbjct: 541 RHESLTQWRTRIESSGFDPVHLGSNAFKQASMLLALFAGGEGYRVEENNGCLMLGWHTRP 597

BLAST of MS027541 vs. ExPASy TrEMBL
Match: A0A1S3CDW7 (DELLA protein OS=Cucumis melo OX=3656 GN=LOC103499322 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 7.8e-253
Identity = 473/618 (76.54%), Postives = 510/618 (82.52%), Query Frame = 0

Query: 5   MKREHEDGRSTGGGGGSGSGSGSVYGGGVVKAECSSMSSGKMKMW--EEEDGG------- 64
           MKRE ED RST  G              +VK +CSSMSSGK+KMW  +EE+ G       
Sbjct: 1   MKRELEDDRSTTPGDSR----------RIVKGDCSSMSSGKLKMWHLQEEEAGEDAKDGA 60

Query: 65  -GGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGW 124
            GGMDELLAVLGYKVR+SDMADVA KLEQLEMVMGTA E GISHL+ DTVHYNPSDLS W
Sbjct: 61  AGGMDELLAVLGYKVRSSDMADVALKLEQLEMVMGTAHEHGISHLASDTVHYNPSDLSSW 120

Query: 125 VQNMLSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPPK 184
           VQNMLSEFNN  +   +  P     SN H  IQS+ S FY+DDSEYDLSAIPGVA LPPK
Sbjct: 121 VQNMLSEFNNSTNHF-NPPPQSSPYSNCHHPIQSTKSCFYDDDSEYDLSAIPGVAVLPPK 180

Query: 185 HSSSSAGTETNSRKRLK--TSTSSIG-----ASSSPPFVATAGIVSEPSRPVVVV--DSQ 244
                  T+TNSRKRLK  T +SS+      +SSSPPF +T GI SE SRPV+VV  +SQ
Sbjct: 181 DE-----TQTNSRKRLKIETQSSSVNLLPSPSSSSPPFASTPGIASESSRPVMVVEEESQ 240

Query: 245 EAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYR 304
           E GIQLVH LMACAEAVQQENM L DALVKHIG L  SQAGAMRKVATYFA+ALARRIYR
Sbjct: 241 ETGIQLVHALMACAEAVQQENMKLADALVKHIGFLATSQAGAMRKVATYFAQALARRIYR 300

Query: 305 IYSPQDGL---YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQ 364
           IYSPQDGL   YSD LQ HFYETCPYLKFAHFTANQAILEAFATA RVHVIDFSLNQGMQ
Sbjct: 301 IYSPQDGLYSSYSDPLQMHFYETCPYLKFAHFTANQAILEAFATAARVHVIDFSLNQGMQ 360

Query: 365 WPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVC 424
           WP LMQALALR GGPP FRLTGIGPP  +N+  SL+QVGWKL++ AEAIGV F+FNHIVC
Sbjct: 361 WPALMQALALRPGGPPAFRLTGIGPPQPENAAGSLQQVGWKLAQMAEAIGVDFEFNHIVC 420

Query: 425 SNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQE 484
           SNL DLD AAL+I PA VEAVAVNSVFDLHRLLARPGAIEKVLGSIK  KPKIVTIVEQE
Sbjct: 421 SNLADLDSAALEIRPAAVEAVAVNSVFDLHRLLARPGAIEKVLGSIKTTKPKIVTIVEQE 480

Query: 485 ANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEGT 544
           ANHNGPVF+DRFTEALHYYSNLFDSLEG S+GFEP +EDV++SE YLG+QICNVVACEGT
Sbjct: 481 ANHNGPVFLDRFTEALHYYSNLFDSLEGSSSGFEPGSEDVLLSEVYLGKQICNVVACEGT 540

Query: 545 DRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGW 601
           +RVERHE+L QWRTRMESAGFDPVH+GSNAFKQASMLLAL+AGGEGYRVEENNGCLMLGW
Sbjct: 541 NRVERHESLTQWRTRMESAGFDPVHLGSNAFKQASMLLALFAGGEGYRVEENNGCLMLGW 600

BLAST of MS027541 vs. TAIR 10
Match: AT2G01570.1 (GRAS family transcription factor family protein )

HSP 1 Score: 647.9 bits (1670), Expect = 8.2e-186
Identity = 365/615 (59.35%), Postives = 433/615 (70.41%), Query Frame = 0

Query: 5   MKREHEDGRSTGGGGGSGSGSGSVYGGGVVKAECSSMSSGKMKM-WEEEDGGGGM-DELL 64
           MKR+H   +      G+ S S             SS+S  KM M  +EEDGGG M DELL
Sbjct: 1   MKRDHHQFQGRLSNHGTSSSS-------------SSISKDKMMMVKKEEDGGGNMDDELL 60

Query: 65  AVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWVQNMLSEF 124
           AVLGYKVR+S+MA+VA KLEQLE +M   QEDG+SHL+ DTVHYNPS+L  W+ NMLSE 
Sbjct: 61  AVLGYKVRSSEMAEVALKLEQLETMMSNVQEDGLSHLATDTVHYNPSELYSWLDNMLSEL 120

Query: 125 NNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPG--VAALPPKHSSSSA 184
           N          P    +SN    +  SP       S+YDL  IPG  +   P   SSSS+
Sbjct: 121 N---------PPPLPASSNGLDPVLPSPEICGFPASDYDLKVIPGNAIYQFPAIDSSSSS 180

Query: 185 GTETNSRKRLK---------TSTSS-------IGASSSPPFVATAGIVSEPSRPVVVVDS 244
               N  KRLK         TSTS+       IG + +     T     E +R V++VDS
Sbjct: 181 ---NNQNKRLKSCSSPDSMVTSTSTGTQIGGVIGTTVTTTTTTTTA-AGESTRSVILVDS 240

Query: 245 QEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIY 304
           QE G++LVH LMACAEA+QQ N+TL +ALVK IG L  SQAGAMRKVATYFAEALARRIY
Sbjct: 241 QENGVRLVHALMACAEAIQQNNLTLAEALVKQIGCLAVSQAGAMRKVATYFAEALARRIY 300

Query: 305 RIYSPQ---DGLYSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGM 364
           R+  PQ   D   SD LQ HFYETCPYLKFAHFTANQAILEAF    RVHVIDFS+NQG+
Sbjct: 301 RLSPPQNQIDHCLSDTLQMHFYETCPYLKFAHFTANQAILEAFEGKKRVHVIDFSMNQGL 360

Query: 365 QWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIV 424
           QWP LMQALALR GGPP FRLTGIGPP  DNS D L +VG KL++ AEAI V+F++   V
Sbjct: 361 QWPALMQALALREGGPPTFRLTGIGPPAPDNS-DHLHEVGCKLAQLAEAIHVEFEYRGFV 420

Query: 425 CSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQ 484
            ++L DLD + L++ P++ EAVAVNSVF+LH+LL RPG IEKVLG +K++KP I T+VEQ
Sbjct: 421 ANSLADLDASMLELRPSDTEAVAVNSVFELHKLLGRPGGIEKVLGVVKQIKPVIFTVVEQ 480

Query: 485 EANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEG 544
           E+NHNGPVF+DRFTE+LHYYS LFDSLEG      P ++D VMSE YLG+QICN+VACEG
Sbjct: 481 ESNHNGPVFLDRFTESLHYYSTLFDSLEGV-----PNSQDKVMSEVYLGKQICNLVACEG 540

Query: 545 TDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLG 597
            DRVERHETL+QW  R  S+G  P H+GSNAFKQASMLL+++  G+GYRVEE+NGCLMLG
Sbjct: 541 PDRVERHETLSQWGNRFGSSGLAPAHLGSNAFKQASMLLSVFNSGQGYRVEESNGCLMLG 583

BLAST of MS027541 vs. TAIR 10
Match: AT1G14920.1 (GRAS family transcription factor family protein )

HSP 1 Score: 638.3 bits (1645), Expect = 6.5e-183
Identity = 341/564 (60.46%), Postives = 409/564 (72.52%), Query Frame = 0

Query: 45  KMKMWEEEDGGGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTV 104
           K  M  EED G GMDELLAVLGYKVR+S+MADVAQKLEQLE++M   QED +S L+ +TV
Sbjct: 14  KTMMMNEEDDGNGMDELLAVLGYKVRSSEMADVAQKLEQLEVMMSNVQEDDLSQLATETV 73

Query: 105 HYNPSDLSGWVQNMLSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSA 164
           HYNP++L  W+ +ML++ N                          PS     ++EYDL A
Sbjct: 74  HYNPAELYTWLDSMLTDLN-------------------------PPS----SNAEYDLKA 133

Query: 165 IPGVAAL------PPKHSSSSAGTET-NSRKRLKTSTSSIGASSSPPFVATAGIVSEPSR 224
           IPG A L          S+   G +T  + KRLK S            V T    +E +R
Sbjct: 134 IPGDAILNQFAIDSASSSNQGGGGDTYTTNKRLKCSNG---------VVETTTATAESTR 193

Query: 225 PVVVVDSQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAE 284
            VV+VDSQE G++LVH L+ACAEAVQ+EN+T+ +ALVK IG L  SQ GAMRKVATYFAE
Sbjct: 194 HVVLVDSQENGVRLVHALLACAEAVQKENLTVAEALVKQIGFLAVSQIGAMRKVATYFAE 253

Query: 285 ALARRIYRI---YSPQDGLYSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVID 344
           ALARRIYR+    SP D   SD LQ HFYETCPYLKFAHFTANQAILEAF    RVHVID
Sbjct: 254 ALARRIYRLSPSQSPIDHSLSDTLQMHFYETCPYLKFAHFTANQAILEAFQGKKRVHVID 313

Query: 345 FSLNQGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVK 404
           FS++QG+QWP LMQALALR GGPP FRLTGIGPP  DN  D L +VG KL+  AEAI V+
Sbjct: 314 FSMSQGLQWPALMQALALRPGGPPVFRLTGIGPPAPDN-FDYLHEVGCKLAHLAEAIHVE 373

Query: 405 FKFNHIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPK 464
           F++   V + L DLD + L++ P+E+E+VAVNSVF+LH+LL RPGAI+KVLG + ++KP+
Sbjct: 374 FEYRGFVANTLADLDASMLELRPSEIESVAVNSVFELHKLLGRPGAIDKVLGVVNQIKPE 433

Query: 465 IVTIVEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQIC 524
           I T+VEQE+NHN P+F+DRFTE+LHYYS LFDSLEG      P+ +D VMSE YLG+QIC
Sbjct: 434 IFTVVEQESNHNSPIFLDRFTESLHYYSTLFDSLEGV-----PSGQDKVMSEVYLGKQIC 493

Query: 525 NVVACEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEEN 584
           NVVAC+G DRVERHETL+QWR R  SAGF   H+GSNAFKQASMLLAL+ GGEGYRVEE+
Sbjct: 494 NVVACDGPDRVERHETLSQWRNRFGSAGFAAAHIGSNAFKQASMLLALFNGGEGYRVEES 533

Query: 585 NGCLMLGWHTRPLIATSAWQLAAD 599
           +GCLMLGWHTRPLIATSAW+L+ +
Sbjct: 554 DGCLMLGWHTRPLIATSAWKLSTN 533

BLAST of MS027541 vs. TAIR 10
Match: AT1G66350.1 (RGA-like 1 )

HSP 1 Score: 616.3 bits (1588), Expect = 2.6e-176
Identity = 347/594 (58.42%), Postives = 409/594 (68.86%), Query Frame = 0

Query: 5   MKREHEDGRSTGGGGGSGSGSGSVYGGGVVKAECSSMSSGKMKMWEEEDGGGGMDELLAV 64
           MKREH    S+ G GGS S +       V+K E +                 G+DELL V
Sbjct: 1   MKREHNHRESSAGEGGSSSMT------TVIKEEAA-----------------GVDELLVV 60

Query: 65  LGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTVHYNPSDLSGWVQNMLSEFNN 124
           LGYKVR+SDMADVA KLEQLEMV+G    DGIS+LS +TVHYNPSDLSGWV++MLS+   
Sbjct: 61  LGYKVRSSDMADVAHKLEQLEMVLG----DGISNLSDETVHYNPSDLSGWVESMLSDL-- 120

Query: 125 FPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLSAIPGVAALPPKHSSSSAGTET 184
                   DP         ++IQ  P      DSEYDL AIPG A  P         T  
Sbjct: 121 --------DP---------TRIQEKP------DSEYDLRAIPGSAVYP----RDEHVTRR 180

Query: 185 NSRKRLKTSTSSIGASSSPPFVATAGIVSEPSRPVVVVDSQEAGIQLVHTLMACAEAVQQ 244
           + R R+++  SS                   +R VVV+DSQE G++LVH L+ACAEAVQQ
Sbjct: 181 SKRTRIESELSS-------------------TRSVVVLDSQETGVRLVHALLACAEAVQQ 240

Query: 245 ENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRIYRIYSPQD---GLYSDLLQNH 304
            N+ L DALVKH+GLL +SQAGAMRKVATYFAE LARRIYRIY   D     +SD LQ H
Sbjct: 241 NNLKLADALVKHVGLLASSQAGAMRKVATYFAEGLARRIYRIYPRDDVALSSFSDTLQIH 300

Query: 305 FYETCPYLKFAHFTANQAILEAFATANRVHVIDFSLNQGMQWPVLMQALALRVGGPPEFR 364
           FYE+CPYLKFAHFTANQAILE FATA +VHVID  LN G+QWP L+QALALR  GPP+FR
Sbjct: 301 FYESCPYLKFAHFTANQAILEVFATAEKVHVIDLGLNHGLQWPALIQALALRPNGPPDFR 360

Query: 365 LTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFKFNHIVCSNLEDLDPAALDILPAEVE 424
           LTGIG  L+D     +++VGWKL + A  IGV F+F  I  +NL DL P  LDI P  +E
Sbjct: 361 LTGIGYSLTD-----IQEVGWKLGQLASTIGVNFEFKSIALNNLSDLKPEMLDIRPG-LE 420

Query: 425 AVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIVTIVEQEANHNGPVFVDRFTEALHYY 484
           +VAVNSVF+LHRLLA PG+I+K L +IK ++P I+T+VEQEANHNG VF+DRFTE+LHYY
Sbjct: 421 SVAVNSVFELHRLLAHPGSIDKFLSTIKSIRPDIMTVVEQEANHNGTVFLDRFTESLHYY 480

Query: 485 SNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNVVACEGTDRVERHETLAQWRTRMESA 544
           S+LFDSLEG      P ++D VMSE +LGRQI N+VACEG DRVERHETL QWR R    
Sbjct: 481 SSLFDSLEG------PPSQDRVMSELFLGRQILNLVACEGEDRVERHETLNQWRNRFGLG 507

Query: 545 GFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNGCLMLGWHTRPLIATSAWQL 596
           GF PV +GSNA+KQASMLLALYAG +GY VEEN GCL+LGW TRPLIATSAW++
Sbjct: 541 GFKPVSIGSNAYKQASMLLALYAGADGYNVEENEGCLLLGWQTRPLIATSAWRI 507

BLAST of MS027541 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 607.4 bits (1565), Expect = 1.2e-173
Identity = 331/560 (59.11%), Postives = 405/560 (72.32%), Query Frame = 0

Query: 45  KMKMWEEEDGGGGMDELLAVLGYKVRASDMADVAQKLEQLEMVMGTAQEDGISHLSCDTV 104
           K K  ++ +     DELLAVLGYKVR+S+MA+VAQKLEQLEMV+  + +D  S +  D+V
Sbjct: 30  KKKADDDNNNSNMDDELLAVLGYKVRSSEMAEVAQKLEQLEMVL--SNDDVGSTVLNDSV 89

Query: 105 HYNPSDLSGWVQNMLSEFNN-FPSDLQHRDPLCLHNSNSHSQIQSSPSRFYNDDSEYDLS 164
           HYNPSDLS WV++MLSE NN   SDL                     +R   D SEYDL 
Sbjct: 90  HYNPSDLSNWVESMLSELNNPASSDL-------------------DTTRSCVDRSEYDLR 149

Query: 165 AIPGVAALPPKHSSSSAGTETNSRKRLKTSTSSIGASSSPPFVATAGIVSEPSRPVVVVD 224
           AIPG++A P          E  S KR++  +      SS           E +R VV+VD
Sbjct: 150 AIPGLSAFP---KEEEVFDEEASSKRIRLGS---WCESS----------DESTRSVVLVD 209

Query: 225 SQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRKVATYFAEALARRI 284
           SQE G++LVH L+ACAEA+ QEN+ L DALVK +G L  SQAGAM KVATYFA+ALARRI
Sbjct: 210 SQETGVRLVHALVACAEAIHQENLNLADALVKRVGTLAGSQAGAMGKVATYFAQALARRI 269

Query: 285 YRIYSPQDGL-------YSDLLQNHFYETCPYLKFAHFTANQAILEAFATANRVHVIDFS 344
           YR Y+ +  +       + ++L+ HFYE+CPYLKFAHFTANQAILEA  TA RVHVID  
Sbjct: 270 YRDYTAETDVCAAVNPSFEEVLEMHFYESCPYLKFAHFTANQAILEAVTTARRVHVIDLG 329

Query: 345 LNQGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRTAEAIGVKFK 404
           LNQGMQWP LMQALALR GGPP FRLTGIGPP ++NS DSL+Q+GWKL++ A+ +GV+F+
Sbjct: 330 LNQGMQWPALMQALALRPGGPPSFRLTGIGPPQTENS-DSLQQLGWKLAQFAQNMGVEFE 389

Query: 405 FNHIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGSIKRMKPKIV 464
           F  +   +L DL+P   +  P E E + VNSVF+LHRLLAR G+IEK+L ++K +KP IV
Sbjct: 390 FKGLAAESLSDLEPEMFETRP-ESETLVVNSVFELHRLLARSGSIEKLLNTVKAIKPSIV 449

Query: 465 TIVEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEFYLGRQICNV 524
           T+VEQEANHNG VF+DRF EALHYYS+LFDSLE   + +   ++D VMSE YLGRQI NV
Sbjct: 450 TVVEQEANHNGIVFLDRFNEALHYYSSLFDSLE---DSYSLPSQDRVMSEVYLGRQILNV 509

Query: 525 VACEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGEGYRVEENNG 584
           VA EG+DRVERHET AQWR RM+SAGFDP+H+GS+AFKQASMLL+LYA G+GYRVEEN+G
Sbjct: 510 VAAEGSDRVERHETAAQWRIRMKSAGFDPIHLGSSAFKQASMLLSLYATGDGYRVEENDG 547

Query: 585 CLMLGWHTRPLIATSAWQLA 597
           CLM+GW TRPLI TSAW+LA
Sbjct: 570 CLMIGWQTRPLITTSAWKLA 547

BLAST of MS027541 vs. TAIR 10
Match: AT5G17490.1 (RGA-like protein 3 )

HSP 1 Score: 561.2 bits (1445), Expect = 1.0e-159
Identity = 313/571 (54.82%), Postives = 393/571 (68.83%), Query Frame = 0

Query: 34  VKAECSSMSSGKMKMWEEEDGGGG---MDELLAVLGYKVRASDMADVAQKLEQLEMVMGT 93
           V+ E  SM    ++  E   GGGG   MDE LAVLGYKVR+SDMADVAQKLEQLEMV+  
Sbjct: 10  VEEEAPSM----VEKLENGCGGGGDDNMDEFLAVLGYKVRSSDMADVAQKLEQLEMVLSN 69

Query: 94  AQEDGISHLSCDTVHYNPSDLSGWVQNMLSEFNNFPSDLQHRDPLCLHNSNSHSQIQSSP 153
                 S+   DTVHYNPSDLSGW Q+MLS+ N +P                        
Sbjct: 70  DIASS-SNAFNDTVHYNPSDLSGWAQSMLSDLNYYP------------------------ 129

Query: 154 SRFYNDDSEYDLSAIPGVAALPPKHSSSSAGTETNSRKRLKTSTSSIGASSSPPFVATAG 213
                   + D + I  +  +       S+ + +N R RL     S              
Sbjct: 130 --------DLDPNRICDLRPITDDDECCSSNSNSNKRIRLGPWCDS-------------- 189

Query: 214 IVSEPSRPVVVVDSQEAGIQLVHTLMACAEAVQQENMTLGDALVKHIGLLMASQAGAMRK 273
           + SE +R VV++  +E G++LV  L+ACAEAVQ EN++L DALVK +GLL ASQAGAM K
Sbjct: 190 VTSESTRSVVLI--EETGVRLVQALVACAEAVQLENLSLADALVKRVGLLAASQAGAMGK 249

Query: 274 VATYFAEALARRIYRIY---SPQDGLYSDLLQNHFYETCPYLKFAHFTANQAILEAFATA 333
           VATYFAEALARRIYRI+   +  D  + ++LQ +FY++CPYLKFAHFTANQAILEA  T+
Sbjct: 250 VATYFAEALARRIYRIHPSAAAIDPSFEEILQMNFYDSCPYLKFAHFTANQAILEAVTTS 309

Query: 334 NRVHVIDFSLNQGMQWPVLMQALALRVGGPPEFRLTGIGPPLSDNSTDSLEQVGWKLSRT 393
             VHVID  LNQGMQWP LMQALALR GGPP FRLTG+G P   ++ + ++++GWKL++ 
Sbjct: 310 RVVHVIDLGLNQGMQWPALMQALALRPGGPPSFRLTGVGNP---SNREGIQELGWKLAQL 369

Query: 394 AEAIGVKFKFNHIVCSNLEDLDPAALDILPAEVEAVAVNSVFDLHRLLARPGAIEKVLGS 453
           A+AIGV+FKFN +    L DL+P   +    E E + VNSVF+LH +L++PG+IEK+L +
Sbjct: 370 AQAIGVEFKFNGLTTERLSDLEPDMFE-TRTESETLVVNSVFELHPVLSQPGSIEKLLAT 429

Query: 454 IKRMKPKIVTIVEQEANHNGPVFVDRFTEALHYYSNLFDSLEGYSNGFEPANEDVVMSEF 513
           +K +KP +VT+VEQEANHNG VF+DRF EALHYYS+LFDSLE   +G    ++D VMSE 
Sbjct: 430 VKAVKPGLVTVVEQEANHNGDVFLDRFNEALHYYSSLFDSLE---DGVVIPSQDRVMSEV 489

Query: 514 YLGRQICNVVACEGTDRVERHETLAQWRTRMESAGFDPVHMGSNAFKQASMLLALYAGGE 573
           YLGRQI N+VA EG+DR+ERHETLAQWR RM SAGFDPV++GS+AFKQAS+LLAL  GG+
Sbjct: 490 YLGRQILNLVATEGSDRIERHETLAQWRKRMGSAGFDPVNLGSDAFKQASLLLALSGGGD 520

Query: 574 GYRVEENNGCLMLGWHTRPLIATSAWQLAAD 599
           GYRVEEN+G LML W T+PLIA SAW+LAA+
Sbjct: 550 GYRVEENDGSLMLAWQTKPLIAASAWKLAAE 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022961073.16.1e-26077.81DELLA protein GAI-like [Cucurbita moschata] >XP_022961085.1 DELLA protein GAI-li... [more]
XP_023550819.14.0e-25978.41DELLA protein GAI-like [Cucurbita pepo subsp. pepo][more]
XP_023546027.18.9e-25977.49DELLA protein GAI-like [Cucurbita pepo subsp. pepo][more]
KAG6602029.13.4e-25877.08DELLA protein GAI, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6579033.14.4e-25878.34DELLA protein GAI, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q84TQ76.3e-20767.01DELLA protein GAI OS=Gossypium hirsutum OX=3635 GN=GAI PE=2 SV=1[more]
Q7Y1B66.9e-20666.55DELLA protein GAI OS=Solanum lycopersicum OX=4081 GN=GAI PE=2 SV=1[more]
A0A396IUP15.5e-20363.05DELLA protein 1 OS=Medicago truncatula OX=3880 GN=DELLA1 PE=1 SV=1[more]
Q8S4W72.8e-19965.50DELLA protein GAI1 OS=Vitis vinifera OX=29760 GN=GAI1 PE=2 SV=1[more]
Q5BN227.0e-19060.69DELLA protein RGA2 OS=Brassica campestris OX=3711 GN=RGA2 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HAU03.0e-26077.81DELLA protein OS=Cucurbita moschata OX=3662 GN=LOC111461688 PE=3 SV=1[more]
A0A6J1FFR83.6e-25878.30DELLA protein OS=Cucurbita moschata OX=3662 GN=LOC111445074 PE=3 SV=1[more]
A0A6J1JHZ81.1e-25777.92DELLA protein OS=Cucurbita maxima OX=3661 GN=LOC111487159 PE=3 SV=1[more]
A0A6J1K1741.3e-25577.69DELLA protein OS=Cucurbita maxima OX=3661 GN=LOC111489223 PE=3 SV=1[more]
A0A1S3CDW77.8e-25376.54DELLA protein OS=Cucumis melo OX=3656 GN=LOC103499322 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G01570.18.2e-18659.35GRAS family transcription factor family protein [more]
AT1G14920.16.5e-18360.46GRAS family transcription factor family protein [more]
AT1G66350.12.6e-17658.42RGA-like 1 [more]
AT3G03450.11.2e-17359.11RGA-like 2 [more]
AT5G17490.11.0e-15954.82RGA-like protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 74..94
NoneNo IPR availableSMARTSM01129DELLA_2coord: 59..132
e-value: 4.5E-38
score: 142.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..201
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..201
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
IPR038088DELLA, N-terminal domain superfamilyGENE3D1.10.10.1290coord: 57..122
e-value: 8.2E-37
score: 127.0
IPR021914Transcriptional factor DELLA, N-terminalPFAMPF12041DELLAcoord: 59..126
e-value: 3.0E-37
score: 126.3
IPR005202Transcription factor GRASPFAMPF03514GRAScoord: 231..594
e-value: 1.2E-131
score: 439.1
IPR005202Transcription factor GRASPANTHERPTHR31636OSJNBA0084A10.13 PROTEIN-RELATEDcoord: 53..594
IPR005202Transcription factor GRASPROSITEPS50985GRAScoord: 222..594
score: 99.056274
IPR030006Transcriptional factor DELLAPANTHERPTHR31636:SF47DELLA PROTEIN RGL1coord: 53..594

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS027541.1MS027541.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0003712 transcription coregulator activity