MS027106 (gene) Bitter gourd (TR) v1

Overview
NameMS027106
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGDSL esterase/lipase CPRD49-like
Locationscaffold563: 387776 .. 389979 (+)
RNA-Seq ExpressionMS027106
SyntenyMS027106
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAGGGCCAGTGAGACCTCGCTTTGTCTTCTTTGGTTCTTCCATAGTGGAGTTCAGCTTTGACGATGGTGGATGGGGTGCTGGTCTTGCCAATTTATACGCTCGCAGGGTATTTTTGCTCTTAACTTTCTTATCTCTATTCATTGGAAATGAAATGAGGTTGTTTAAATGCATGATCTCCAACTCTAATTACTATATTAAAATGCCACTCAACTAAAAAAAACTTAAAAGAATTTTGGTAAATTTAATTTTTAATTCATACCTTTCTATTTTAAATCCTAATCAAAGTTCCTAATACAACTCCACAGGCAGATATGGTTTTACGAGGCTATGGTGGTTGGAATTCAAGACAGGCTCTAAGAGTTGTGAAAGAAATCTTTCCAGCGGTACACACACCTTATGTGAATTCTCATAATTATGCTAACTAAATTTTTTTATAAAAAAATGAAATCAAGTTTGGTTATTGAATGTTCACAATTTTATTAAATAAATCATCAAAATTTTGAAAGGTGCATAGTCTATAAACTTCGAATCGTATCTAATAGATTTCTAAATTTTAAAAATTGTCAAATAATTCAATTCTAAAACTTTCAAATTTTTTTTTATCTAAGAAAGTCTTTAGACATTTTCTTTTATTTAATAGGTAAAAAATTGTGATCTATTAAACATAAAATCGAAAATTTAGAGACTTATTAGACATTTTCTTAAAAAAATTTAACAACTATGTCTTCTTTAACCATTACCTTCACTAACCTGATAAAAAATCACATTATAATTACTAACCATTACTAACATCTCTATTTATAAATCTCTAAATAGTCTTTGGGAGGATATTCTTGTCTCTGCATCTACTTGGTCTTCAAAATCAAATTTATTTTATAATTACGATATATCTATTATTTCTCTCAATTGGAGTGCTTTCATCTAAACCTCCTCTTTTGTAGGTATGTCTTTCATATACTTTAAATGAAATATGGTACCTCCTCTACTAAATAAAAGTTACTAATATCTCTATTCGTGGTGTGTTAGGACAATCCGATCAAACCTGCTTTGGCCATAGTCTACTTTGGTGGTAATGATGCAATGATTCCTTTTCCCGGTAGCCCTCACGTGCCATATTGTGAATTCCTCGACAACATGACCCAGATCGCCCTCCATATCAAGGTCAACTACCCTAAAATATTCTATTTTCTTTGAAAATTAAAAAAAAAATCATTTTTAATTTTTACTTAAAAAAAATTAATTAAAATTTCAAATGTGTTACCTAACAAGGTTTTAAATGTCGATGTCAATGGAAATGTGGAGGTCTCAATTTTATGGAAATATCGATAAAATGTCGATGTTGATGGATATTTCTATAAAAGTTATAGAAATTAAAAGATCGTTAACTTAAAAAATAACAAATATTGATATTATGTATATATTAGTGACATTTTGTTGATATTTTGTAGATTTTTTTCTACTATGATATTGATATTATGTAACCGATGGAAAATGTCGATTTACCTTCATGTTGATGTCGAATTCATGAAAACATGGACACATTGATTTAAAAATCGACGTGTCGATGGATATTTAGAAGCGGAGTTACTTGACTTTTACGTTGTATAACACTCTTCAGGGCATCTCAGAGACCACTCGTTGCCTCTTCCTCTCGGTTCCGCCCGTAAACAGTGAACAACTTCACGAAATAAGGTGTAGACTTCAAAATCATATATATTAATTATTGAATTCGATGAACATGAATTTTTATATTTCTTGGATTGCTGATAGCGATCTTGTTTTTCTGATTGGCCCAGTCCGGGATTGGAGAAACTTCGACCAATGGAGAATTGTGAGAAGTATTCAGAAGGCACTATTAATATTTGCAAGCAATTGGGCATTAAATACATTGATTTGTTTAATGCAATTCAGCGGAGACCAGATTGGAAAAATTTCTTCACGTTGGTTTCTAGTTCATAGTTCCTACTATGCCTTTAAATTGAATTTGTTAGCAAAAATATTATAACTTTTTTTTTTAAAACTTATTTTAGGGATGGAGTTCATTTTAACCCAAAAGGAAGCGAGGCAGTTGAAGAGGAAATTTTGAAGGTGTTGAAAGAGGCAGAGTGGGTACCAAGCTTGCACTGGCAATCGATGCCAAAGGAATACGAAGACATCCATCCCATTCGCTTTAATAGCGCAAAAGTGCTTCTGGATTAA

mRNA sequence

ATGGTAGGGCCAGTGAGACCTCGCTTTGTCTTCTTTGGTTCTTCCATAGTGGAGTTCAGCTTTGACGATGGTGGATGGGGTGCTGGTCTTGCCAATTTATACGCTCGCAGGGACAATCCGATCAAACCTGCTTTGGCCATAGTCTACTTTGGTGGTAATGATGCAATGATTCCTTTTCCCGGTAGCCCTCACGTGCCATATTGTGAATTCCTCGACAACATGACCCAGATCGCCCTCCATATCAAGGGCATCTCAGAGACCACTCGTTGCCTCTTCCTCTCGGTTCCGCCCGTAAACAGTGAACAACTTCACGAAATAAGTCCGGGATTGGAGAAACTTCGACCAATGGAGAATTGTGAGAAGTATTCAGAAGGCACTATTAATATTTGCAAGCAATTGGGCATTAAATACATTGATTTGTTTAATGCAATTCAGCGGAGACCAGATTGGAAAAATTTCTTCACGGATGGAGTTCATTTTAACCCAAAAGGAAGCGAGGCAGTTGAAGAGGAAATTTTGAAGGTGTTGAAAGAGGCAGAGTGGGTACCAAGCTTGCACTGGCAATCGATGCCAAAGGAATACGAAGACATCCATCCCATTCGCTTTAATAGCGCAAAAGTGCTTCTGGATTAA

Coding sequence (CDS)

ATGGTAGGGCCAGTGAGACCTCGCTTTGTCTTCTTTGGTTCTTCCATAGTGGAGTTCAGCTTTGACGATGGTGGATGGGGTGCTGGTCTTGCCAATTTATACGCTCGCAGGGACAATCCGATCAAACCTGCTTTGGCCATAGTCTACTTTGGTGGTAATGATGCAATGATTCCTTTTCCCGGTAGCCCTCACGTGCCATATTGTGAATTCCTCGACAACATGACCCAGATCGCCCTCCATATCAAGGGCATCTCAGAGACCACTCGTTGCCTCTTCCTCTCGGTTCCGCCCGTAAACAGTGAACAACTTCACGAAATAAGTCCGGGATTGGAGAAACTTCGACCAATGGAGAATTGTGAGAAGTATTCAGAAGGCACTATTAATATTTGCAAGCAATTGGGCATTAAATACATTGATTTGTTTAATGCAATTCAGCGGAGACCAGATTGGAAAAATTTCTTCACGGATGGAGTTCATTTTAACCCAAAAGGAAGCGAGGCAGTTGAAGAGGAAATTTTGAAGGTGTTGAAAGAGGCAGAGTGGGTACCAAGCTTGCACTGGCAATCGATGCCAAAGGAATACGAAGACATCCATCCCATTCGCTTTAATAGCGCAAAAGTGCTTCTGGATTAA

Protein sequence

MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYARRDNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLSVPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFFTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIRFNSAKVLLD
Homology
BLAST of MS027106 vs. NCBI nr
Match: XP_022145670.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 435.6 bits (1119), Expect = 2.4e-118
Identity = 210/236 (88.98%), Postives = 210/236 (88.98%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYARR----------------------- 60
           MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYARR                       
Sbjct: 61  MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYARRADMVLRGYGGWNSRQALRVVKEI 120

Query: 61  ---DNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS 120
              DNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS
Sbjct: 121 FPVDNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS 180

Query: 121 VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF 180
           VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF
Sbjct: 181 VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF 240

Query: 181 TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIRFNSAKVLLD 211
           TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIRFNSAKVLLD
Sbjct: 241 TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIRFNSAKVLLD 296

BLAST of MS027106 vs. NCBI nr
Match: XP_022145498.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 260.4 bits (664), Expect = 1.4e-65
Identity = 127/241 (52.70%), Postives = 162/241 (67.22%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGPVRP+FV FGSSIV+FSF D GWGA +A+LY+R                        
Sbjct: 70  MVGPVRPKFVLFGSSIVQFSFGDRGWGADIADLYSRQADVILRGYGGWNSSRALQVVDEI 129

Query: 61  --RDNPIKPALAIVYFGGNDAMI--PFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLF 120
             +D+ I+P+L IVYFGGNDA+I  PFPG  HVP  EF++NMT I  HIK +S TTR +F
Sbjct: 130 FPKDSIIQPSLVIVYFGGNDAVIPLPFPGGSHVPLPEFINNMTNIVCHIKSLSATTRSIF 189

Query: 121 LSVPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKN 180
           L+ PPVNSEQ+ E+ PGLEKLR MEN E Y++  I+IC+ +G++Y+DLF A+QRR DW  
Sbjct: 190 LTPPPVNSEQIRELMPGLEKLRTMENLEIYTDAIIDICRDIGVEYVDLFRAVQRRDDWLT 249

Query: 181 FFTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPI---RFNSAKVLL 211
           FFTDGVHFN +GS  V EEI   +  A WVPSL   SMP E+E+I  +    F  ++++L
Sbjct: 250 FFTDGVHFNEEGSRVVFEEIKNTINRANWVPSLAAGSMPIEFENIRRLGSFTFEGSRIIL 309

BLAST of MS027106 vs. NCBI nr
Match: XP_022145518.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 259.2 bits (661), Expect = 3.0e-65
Identity = 126/241 (52.28%), Postives = 164/241 (68.05%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGPVRP+FV FGSSIV++SFDD GWGA +ANLYAR                        
Sbjct: 46  MVGPVRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEV 105

Query: 61  --RDNPIKPALAIVYFGGNDAMI--PFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLF 120
             +D+ I+P+LAIVYFGGNDA+I  PFPG  HVP  +F+DNMT+IA HIK +S TTR +F
Sbjct: 106 FPKDSIIQPSLAIVYFGGNDAIIPLPFPGGSHVPLPQFIDNMTKIACHIKSLSTTTRSIF 165

Query: 121 LSVPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKN 180
           L+ PPVNS+Q+ E+ PGLEKLR MEN E Y++  I+IC+++G++Y+DLF A+Q+R DW  
Sbjct: 166 LTPPPVNSDQIRELLPGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLT 225

Query: 181 FFTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIR---FNSAKVLL 211
           FFTDGVHF  +GS  V +EI   +  A WVPSL   SMP E+ +   +R   F  + ++L
Sbjct: 226 FFTDGVHFTEEGSRVVFDEIKNTINRANWVPSLAVGSMPIEFGNNTGLRSFDFEGSSIIL 285

BLAST of MS027106 vs. NCBI nr
Match: XP_022145497.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 256.5 bits (654), Expect = 2.0e-64
Identity = 130/239 (54.39%), Postives = 162/239 (67.78%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGP+RP+FV FGSSIV+FSF D GWGA +ANLYAR                        
Sbjct: 46  MVGPMRPKFVVFGSSIVQFSFADKGWGAEIANLYARQADVILRGYSGWNSSQALEVVDEV 105

Query: 61  --RDNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS 120
             +D+ I+P+L IVYFGGNDA+I FP S  VP  EF  NMT+IA HIK +S TTR +FL+
Sbjct: 106 FPKDSIIQPSLVIVYFGGNDAVINFPTS--VPLSEFTVNMTKIASHIKNLSTTTRGIFLT 165

Query: 121 VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF 180
            PPVNSEQ+ E+ PGLEKLR MEN EKY++  I+ICK++G++Y+DLF A+QRR DW  FF
Sbjct: 166 PPPVNSEQIRELFPGLEKLRTMENLEKYTDALIDICKEIGVEYVDLFRAVQRRDDWLTFF 225

Query: 181 TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIR---FNSAKVLLD 211
           TDGVHFN KGS  V EEI  ++  A WVPSL   SMP E+ +I  +R   F  + ++LD
Sbjct: 226 TDGVHFNEKGSHVVFEEIKTLINRANWVPSLAAGSMPIEFGNIRGLRTFNFEGSGIILD 282

BLAST of MS027106 vs. NCBI nr
Match: XP_022145517.1 (GDSL esterase/lipase At2g38180-like [Momordica charantia])

HSP 1 Score: 248.4 bits (633), Expect = 5.3e-62
Identity = 122/223 (54.71%), Postives = 151/223 (67.71%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGPVRP+FV FGSSIV+FSFDD GWGA +ANLYAR                        
Sbjct: 46  MVGPVRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKV 105

Query: 61  --RDNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS 120
             +D+ I+PAL IVYFGGNDA I FP S  VP   F DNMT+I  HIK +S TTR +FL+
Sbjct: 106 FPKDSTIQPALVIVYFGGNDASINFPNS--VPLDRFTDNMTKIVDHIKCLSTTTRSIFLT 165

Query: 121 VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF 180
            PP+NSEQL ++ PGLEKLR MEN E Y++  I+ICK+LG++Y+DLF AIQ + DW  FF
Sbjct: 166 PPPINSEQLKQLMPGLEKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFF 225

Query: 181 TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDI 198
            DGVHFN KGS A  +EI  ++ +  W P+L  ++MP E+EDI
Sbjct: 226 RDGVHFNEKGSRAAFDEIKNMINKKNWTPNLDARAMPIEFEDI 266

BLAST of MS027106 vs. ExPASy Swiss-Prot
Match: Q9SRM5 (GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.6e-53
Identity = 106/236 (44.92%), Postives = 148/236 (62.71%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGP RP+ V FGSSIV+ SF  GGWGA L+ +YAR                        
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 61  --RDNPIKPALAIVYFGGNDAMIPFPG--SPHVPYCEFLDNMTQIALHIKGISETTRCLF 120
             +D  ++P+L IVYFGGND+M P      PHVP  E++DNM +IALH++ +S+ TR +F
Sbjct: 61  FPKDAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIF 120

Query: 121 LSVPPVNSEQLHE-ISPGL-EKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDW 180
           LS PPV+  ++ +  SP L E +R  + C+ YS+  + +C++LG++ +DLF+  Q+  DW
Sbjct: 121 LSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 180

Query: 181 KNF-FTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIRFNSA 206
           K   FTDG+H + +GS+ V  EIL+V+KEAEW PSLHW+SMP E+ D  P    SA
Sbjct: 181 KTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFADDSPYDLVSA 236

BLAST of MS027106 vs. ExPASy Swiss-Prot
Match: O80443 (GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 5.2e-52
Identity = 101/223 (45.29%), Postives = 141/223 (63.23%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGPVRP+ V FGSSIV++SF D GWGA LA+LY+R                        
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 61  --RDNPIKPALAIVYFGGNDAMIPFPG--SPHVPYCEFLDNMTQIALHIKGISETTRCLF 120
             +D  I+P+L IVYFGGND+  P P    PHVP  EF++NM +I  H+  +S+ TR +F
Sbjct: 61  FPKDAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIF 120

Query: 121 LSVPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKN 180
           L+ PP+N +Q+  +     K R  E C  Y+E  +N+C+++ +K ID++ AIQ++ DW N
Sbjct: 121 LTPPPMNEKQIEIVFGDAIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDDWLN 180

Query: 181 -FFTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEY 195
             FTDG+HF  K SE V +EILKVL+ A+W PSL+W+S+P E+
Sbjct: 181 SCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEF 223

BLAST of MS027106 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 106.3 bits (264), Expect = 4.3e-22
Identity = 62/226 (27.43%), Postives = 109/226 (48.23%), Query Frame = 0

Query: 5   VRPRFVFFGSSIVEFSFDDGGWGAGLANLYARRDNPI----------------------- 64
           +RP  V FG SI   SF  GGWG+ LA+ Y+R+ + +                       
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 65  ---KPALAIVYFGGNDAMIPFPGS--PHVPYCEFLDNMTQIALHIKGISETTRCLFLSVP 124
               P    ++FG NDA +    S   HVP  E+ DN+ +I  H+K  S T   + ++ P
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 125 PVN-------SEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPD 184
           P++       +E ++      E  R  E    Y++  + + ++LG++ ++L++ +Q   D
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 185 W-KNFFTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEY 195
           W K + +DG+H  P+G+  V +E+ +V +EA   P    + MP ++
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSP----EEMPFDF 222

BLAST of MS027106 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.4e-19
Identity = 64/211 (30.33%), Postives = 96/211 (45.50%), Query Frame = 0

Query: 5   VRPRFVFFGSSIVEFSFDDGGWGAGLANLYARR--------------------------- 64
           +R +   FG SI E SF DGGWGA LA+L  R+                           
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 65  --DNPIKPALAIVYFGGNDAMIP--FPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFL 124
             D    PA   V+FG NDA +P    G  HVP  E+  N+  I   +K     T  + +
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 125 SVPPVNSE-----QLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRP 179
           + PP++ E        E + GL + R  E    Y++  I + ++  I   DL++ +Q+ P
Sbjct: 122 TPPPIDEEARLRYPYIENTTGLPE-RTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIP 181

BLAST of MS027106 vs. ExPASy Swiss-Prot
Match: Q3SZ16 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 54/221 (24.43%), Postives = 99/221 (44.80%), Query Frame = 0

Query: 5   VRPRFVFFGSSIVEFSFDDGGWGAGLANLYARRDNPI----------------------- 64
           V PR + FG SI +FSF  GGWGA LA++  R+ + +                       
Sbjct: 14  VWPRVLLFGDSITQFSFQQGGWGASLADMLVRKCDVLNRGFSGYNTRWAKIILPRLVRKG 73

Query: 65  ----KPALAIVYFGGNDAMIPFPG-SPHVPYCEFLDNMTQIALHIKGISETTRCLFLSVP 124
                P    ++FG ND+ +       HVP  EF+ N+  +  +++ +      L L  P
Sbjct: 74  SGLDSPVAVTIFFGANDSALKDENPKQHVPLEEFVANLRSMVRYLRSVDVPEGRLILITP 133

Query: 125 PVNSEQLHE---ISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQR-RPDWKN 184
           P   E       +  G +  R      +Y+   + + +  G   +DL++ +Q+   D+ +
Sbjct: 134 PPLCEAAWAQECLQQGCKLNRLNSVVGEYARACLQVAQDCGADALDLWSLMQKDGQDFSS 193

Query: 185 FFTDGVHFNPKGSEAVEEEILKVL-KEAEWVPSL--HWQSM 191
           + +DG+H +PKG+E V   +  ++ K+   +P L  +W+ +
Sbjct: 194 YLSDGLHLSPKGNEFVFSHLWPLIEKKVSSLPFLLPYWRDI 234

BLAST of MS027106 vs. ExPASy TrEMBL
Match: A0A6J1CV48 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111015064 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.1e-118
Identity = 210/236 (88.98%), Postives = 210/236 (88.98%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYARR----------------------- 60
           MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYARR                       
Sbjct: 61  MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYARRADMVLRGYGGWNSRQALRVVKEI 120

Query: 61  ---DNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS 120
              DNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS
Sbjct: 121 FPVDNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS 180

Query: 121 VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF 180
           VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF
Sbjct: 181 VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF 240

Query: 181 TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIRFNSAKVLLD 211
           TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIRFNSAKVLLD
Sbjct: 241 TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIRFNSAKVLLD 296

BLAST of MS027106 vs. ExPASy TrEMBL
Match: A0A6J1CW36 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014935 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 6.6e-66
Identity = 127/241 (52.70%), Postives = 162/241 (67.22%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGPVRP+FV FGSSIV+FSF D GWGA +A+LY+R                        
Sbjct: 70  MVGPVRPKFVLFGSSIVQFSFGDRGWGADIADLYSRQADVILRGYGGWNSSRALQVVDEI 129

Query: 61  --RDNPIKPALAIVYFGGNDAMI--PFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLF 120
             +D+ I+P+L IVYFGGNDA+I  PFPG  HVP  EF++NMT I  HIK +S TTR +F
Sbjct: 130 FPKDSIIQPSLVIVYFGGNDAVIPLPFPGGSHVPLPEFINNMTNIVCHIKSLSATTRSIF 189

Query: 121 LSVPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKN 180
           L+ PPVNSEQ+ E+ PGLEKLR MEN E Y++  I+IC+ +G++Y+DLF A+QRR DW  
Sbjct: 190 LTPPPVNSEQIRELMPGLEKLRTMENLEIYTDAIIDICRDIGVEYVDLFRAVQRRDDWLT 249

Query: 181 FFTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPI---RFNSAKVLL 211
           FFTDGVHFN +GS  V EEI   +  A WVPSL   SMP E+E+I  +    F  ++++L
Sbjct: 250 FFTDGVHFNEEGSRVVFEEIKNTINRANWVPSLAAGSMPIEFENIRRLGSFTFEGSRIIL 309

BLAST of MS027106 vs. ExPASy TrEMBL
Match: A0A6J1CW58 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014947 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.5e-65
Identity = 126/241 (52.28%), Postives = 164/241 (68.05%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGPVRP+FV FGSSIV++SFDD GWGA +ANLYAR                        
Sbjct: 46  MVGPVRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEV 105

Query: 61  --RDNPIKPALAIVYFGGNDAMI--PFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLF 120
             +D+ I+P+LAIVYFGGNDA+I  PFPG  HVP  +F+DNMT+IA HIK +S TTR +F
Sbjct: 106 FPKDSIIQPSLAIVYFGGNDAIIPLPFPGGSHVPLPQFIDNMTKIACHIKSLSTTTRSIF 165

Query: 121 LSVPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKN 180
           L+ PPVNS+Q+ E+ PGLEKLR MEN E Y++  I+IC+++G++Y+DLF A+Q+R DW  
Sbjct: 166 LTPPPVNSDQIRELLPGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLT 225

Query: 181 FFTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIR---FNSAKVLL 211
           FFTDGVHF  +GS  V +EI   +  A WVPSL   SMP E+ +   +R   F  + ++L
Sbjct: 226 FFTDGVHFTEEGSRVVFDEIKNTINRANWVPSLAVGSMPIEFGNNTGLRSFDFEGSSIIL 285

BLAST of MS027106 vs. ExPASy TrEMBL
Match: A0A6J1CWS0 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014934 PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 9.5e-65
Identity = 130/239 (54.39%), Postives = 162/239 (67.78%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGP+RP+FV FGSSIV+FSF D GWGA +ANLYAR                        
Sbjct: 46  MVGPMRPKFVVFGSSIVQFSFADKGWGAEIANLYARQADVILRGYSGWNSSQALEVVDEV 105

Query: 61  --RDNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS 120
             +D+ I+P+L IVYFGGNDA+I FP S  VP  EF  NMT+IA HIK +S TTR +FL+
Sbjct: 106 FPKDSIIQPSLVIVYFGGNDAVINFPTS--VPLSEFTVNMTKIASHIKNLSTTTRGIFLT 165

Query: 121 VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF 180
            PPVNSEQ+ E+ PGLEKLR MEN EKY++  I+ICK++G++Y+DLF A+QRR DW  FF
Sbjct: 166 PPPVNSEQIRELFPGLEKLRTMENLEKYTDALIDICKEIGVEYVDLFRAVQRRDDWLTFF 225

Query: 181 TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIR---FNSAKVLLD 211
           TDGVHFN KGS  V EEI  ++  A WVPSL   SMP E+ +I  +R   F  + ++LD
Sbjct: 226 TDGVHFNEKGSHVVFEEIKTLINRANWVPSLAAGSMPIEFGNIRGLRTFNFEGSGIILD 282

BLAST of MS027106 vs. ExPASy TrEMBL
Match: A0A6J1CWU2 (GDSL esterase/lipase At2g38180-like OS=Momordica charantia OX=3673 GN=LOC111014946 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.6e-62
Identity = 122/223 (54.71%), Postives = 151/223 (67.71%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGPVRP+FV FGSSIV+FSFDD GWGA +ANLYAR                        
Sbjct: 46  MVGPVRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKV 105

Query: 61  --RDNPIKPALAIVYFGGNDAMIPFPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFLS 120
             +D+ I+PAL IVYFGGNDA I FP S  VP   F DNMT+I  HIK +S TTR +FL+
Sbjct: 106 FPKDSTIQPALVIVYFGGNDASINFPNS--VPLDRFTDNMTKIVDHIKCLSTTTRSIFLT 165

Query: 121 VPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKNFF 180
            PP+NSEQL ++ PGLEKLR MEN E Y++  I+ICK+LG++Y+DLF AIQ + DW  FF
Sbjct: 166 PPPINSEQLKQLMPGLEKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFF 225

Query: 181 TDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDI 198
            DGVHFN KGS A  +EI  ++ +  W P+L  ++MP E+EDI
Sbjct: 226 RDGVHFNEKGSRAAFDEIKNMINKKNWTPNLDARAMPIEFEDI 266

BLAST of MS027106 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 210.7 bits (535), Expect = 1.1e-54
Identity = 106/236 (44.92%), Postives = 148/236 (62.71%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGP RP+ V FGSSIV+ SF  GGWGA L+ +YAR                        
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 61  --RDNPIKPALAIVYFGGNDAMIPFPG--SPHVPYCEFLDNMTQIALHIKGISETTRCLF 120
             +D  ++P+L IVYFGGND+M P      PHVP  E++DNM +IALH++ +S+ TR +F
Sbjct: 61  FPKDAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIF 120

Query: 121 LSVPPVNSEQLHE-ISPGL-EKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDW 180
           LS PPV+  ++ +  SP L E +R  + C+ YS+  + +C++LG++ +DLF+  Q+  DW
Sbjct: 121 LSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 180

Query: 181 KNF-FTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEYEDIHPIRFNSA 206
           K   FTDG+H + +GS+ V  EIL+V+KEAEW PSLHW+SMP E+ D  P    SA
Sbjct: 181 KTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFADDSPYDLVSA 236

BLAST of MS027106 vs. TAIR 10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 3.7e-53
Identity = 101/223 (45.29%), Postives = 141/223 (63.23%), Query Frame = 0

Query: 1   MVGPVRPRFVFFGSSIVEFSFDDGGWGAGLANLYAR------------------------ 60
           MVGPVRP+ V FGSSIV++SF D GWGA LA+LY+R                        
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 61  --RDNPIKPALAIVYFGGNDAMIPFPG--SPHVPYCEFLDNMTQIALHIKGISETTRCLF 120
             +D  I+P+L IVYFGGND+  P P    PHVP  EF++NM +I  H+  +S+ TR +F
Sbjct: 61  FPKDAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIF 120

Query: 121 LSVPPVNSEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPDWKN 180
           L+ PP+N +Q+  +     K R  E C  Y+E  +N+C+++ +K ID++ AIQ++ DW N
Sbjct: 121 LTPPPMNEKQIEIVFGDAIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDDWLN 180

Query: 181 -FFTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEY 195
             FTDG+HF  K SE V +EILKVL+ A+W PSL+W+S+P E+
Sbjct: 181 SCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEF 223

BLAST of MS027106 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 3.0e-23
Identity = 62/226 (27.43%), Postives = 109/226 (48.23%), Query Frame = 0

Query: 5   VRPRFVFFGSSIVEFSFDDGGWGAGLANLYARRDNPI----------------------- 64
           +RP  V FG SI   SF  GGWG+ LA+ Y+R+ + +                       
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 65  ---KPALAIVYFGGNDAMIPFPGS--PHVPYCEFLDNMTQIALHIKGISETTRCLFLSVP 124
               P    ++FG NDA +    S   HVP  E+ DN+ +I  H+K  S T   + ++ P
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 125 PVN-------SEQLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRPD 184
           P++       +E ++      E  R  E    Y++  + + ++LG++ ++L++ +Q   D
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 185 W-KNFFTDGVHFNPKGSEAVEEEILKVLKEAEWVPSLHWQSMPKEY 195
           W K + +DG+H  P+G+  V +E+ +V +EA   P    + MP ++
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSP----EEMPFDF 222

BLAST of MS027106 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 3.1e-20
Identity = 64/211 (30.33%), Postives = 96/211 (45.50%), Query Frame = 0

Query: 5   VRPRFVFFGSSIVEFSFDDGGWGAGLANLYARR--------------------------- 64
           +R +   FG SI E SF DGGWGA LA+L  R+                           
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 65  --DNPIKPALAIVYFGGNDAMIP--FPGSPHVPYCEFLDNMTQIALHIKGISETTRCLFL 124
             D    PA   V+FG NDA +P    G  HVP  E+  N+  I   +K     T  + +
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 125 SVPPVNSE-----QLHEISPGLEKLRPMENCEKYSEGTINICKQLGIKYIDLFNAIQRRP 179
           + PP++ E        E + GL + R  E    Y++  I + ++  I   DL++ +Q+ P
Sbjct: 122 TPPPIDEEARLRYPYIENTTGLPE-RTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIP 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145670.12.4e-11888.98GDSL esterase/lipase CPRD49-like [Momordica charantia][more]
XP_022145498.11.4e-6552.70GDSL esterase/lipase CPRD49-like [Momordica charantia][more]
XP_022145518.13.0e-6552.28GDSL esterase/lipase CPRD49-like [Momordica charantia][more]
XP_022145497.12.0e-6454.39GDSL esterase/lipase CPRD49-like [Momordica charantia][more]
XP_022145517.15.3e-6254.71GDSL esterase/lipase At2g38180-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SRM51.6e-5344.92GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1[more]
O804435.2e-5245.29GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2... [more]
Q9FM044.3e-2227.43GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2... [more]
Q6NMR94.4e-1930.33GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2... [more]
Q3SZ161.3e-1524.43Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1CV481.1e-11888.98GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111015064 ... [more]
A0A6J1CW366.6e-6652.70GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014935 ... [more]
A0A6J1CW581.5e-6552.28GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014947 ... [more]
A0A6J1CWS09.5e-6554.39GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014934 ... [more]
A0A6J1CWU22.6e-6254.71GDSL esterase/lipase At2g38180-like OS=Momordica charantia OX=3673 GN=LOC1110149... [more]
Match NameE-valueIdentityDescription
AT3G11210.11.1e-5444.92SGNH hydrolase-type esterase superfamily protein [more]
AT2G38180.13.7e-5345.29SGNH hydrolase-type esterase superfamily protein [more]
AT5G62930.13.0e-2327.43SGNH hydrolase-type esterase superfamily protein [more]
AT5G45920.13.1e-2030.33SGNH hydrolase-type esterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013830SGNH hydrolase-type esterase domainPFAMPF13472Lipase_GDSL_2coord: 37..166
e-value: 9.3E-11
score: 42.5
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 37..207
e-value: 2.1E-31
score: 111.0
NoneNo IPR availablePANTHERPTHR14209:SF10GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 1..37
coord: 37..201
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 7..179
IPR045136Isoamyl acetate-hydrolyzing esterase Iah1-likePANTHERPTHR14209ISOAMYL ACETATE-HYDROLYZING ESTERASE 1coord: 1..37
coord: 37..201

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS027106.1MS027106.1mRNA