MS027063 (gene) Bitter gourd (TR) v1

Overview


Name	MS027063
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	WAT1-related protein
Location	scaffold442: 64871 .. 66902 (+)
RNA-Seq Expression	MS027063
Synteny	MS027063

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAAATGATTTGTACAATAGTGTCAATGATTGGAGCTTTTGTGGCTGTTCTCTATAAAGGCCCAATGGTTCTATCCCATCCATCTTCTTCCTCTCAAAATCTTCATCTTCTCCACCTTCACTCTTTCAACTCAGACTGGATTTTTGGTGGCTTCTTGTTTTCTCTTCAGTTCATCTGCTATGCCATTTGGTACATTCTTATGGTAATCTTTTTCTTTTCTTTTCGCTCCAAATTATAATTTTATTTCGCGTTTTTTTTTTCTTATTTTTGTTTTATATGAGATACATGTATATATTTTGTGTTAGGCTTTTTTTTAAGAATATCTTGATACGAATATGGTTCTATGATTTGAGCTTATTTTTAAAATTAGTAAGTTTAGGATTTTAATTTGGTTATTGTAGTTTTGATTTAGTTTCAATTTGGTCCATGTGATTTGGTTAAACTTCATTTGTCATCCCTGTTGTTATCTCTACTTGAATACATCAAATATTTTCTCTCTATATGAATAAATAATGAGCTACGGATTATGTTTAATTTAATTTTACGACAGAGTCAAAAATAGAGAAAAAAGAACGAGTCCAGAATGATCCGTAAGATTTAACAAAACTATAAAGGCCAAGTGAAAATCGAGACCAAATTGAAACTTTTAAGTTACGGGGAGATAGAAATTGAACTGATAAATTGAAAATGAATTATTGCAGACAAAGGCTGTAAAAATGGTTGGGGCTGATTCAATAGTGATGTTCTTGTACTACTTATGCGCCACCGTTTTGTCAACACCCATCGGTTTGTTGGCAGAGAGAGATTTGAGAGCTTGGACACTGCCCACTCGAATGGGACTCTTTGCTGTTATTTATGCGGTAAGTATAAAGAATTACATTTTTAATTATGATTATGAACACCTACTTGTGATTATTTCTCCATTATCTAGTTCTTAAAAACGTTTTCATAATTTAATCTTGAAAACTAAAAAAAGTACAATTTCATTGGATAATTATTTCGTTTTTTATTTTTTATTTATTAAATTGTGCTTGTTTTCCTGTGGTTTCTTTGATATGTTTCAACTTTTTATAAAAATACTTTTTGAATTCTTTGTCAGATTTAAAAAATAAAAACAGACTTTTGAGAGCTAATAGACAGTTTGGTATGCAATATATATTATGTTTCATTCACATTGATTCAATGAATATATGTTATAGATATGTTTTTGATTTTGTGATCTAAATAGTGAAAATATTAAAAATAATATCTGTATGTTTTCAAATTTTCAATTTGATCGTAATGCTTTTTAACATTTTTGTTATCGTTTGAAGAGAAAGATAAATGAAGGAAAAAAGATTGATTATGAAAATCTCATTGGACAAGGATCTCAATTTTAGTTTTATTTGTTTAAGAATTATGTCTTCTTGTATTACCAACATATTTTAAGTCTTAATTAAATTTTAGAAACAATAACAAGGTTTTTAAAACTATATATATTTTTTAGGTTTTAAAATTTTACTTATATTTTGAAAACGTAGGTAACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATCAAAAACAACAAAAAAAAGAAATTTTAATAAATTTTTTTGTAAAGTGCAAATCTGAACATGACTCACAATTATATCTTCTATCATATTTTTTGAACTTAAAAATAAAATCGTTATCAAATAGGTCGGGATTTAAGGATTTGTATTGTAGGGATTGATGGGTCAAGCATTTGGATCAATCGTCCACGCATGGGGTATGAGGATGAGAGGGCCTGTCTATGTCACAAGCTTTAGGCCATTGTCCATTATTATTGCAGCTGCCATGGCTTTTTTTCTTCTTGGTGATGCCCTCTATTTTGGAAGGTGA

mRNA sequence

ATGGCCAAAATGATTTGTACAATAGTGTCAATGATTGGAGCTTTTGTGGCTGTTCTCTATAAAGGCCCAATGGTTCTATCCCATCCATCTTCTTCCTCTCAAAATCTTCATCTTCTCCACCTTCACTCTTTCAACTCAGACTGGATTTTTGGTGGCTTCTTGTTTTCTCTTCAGTTCATCTGCTATGCCATTTGGTACATTCTTATGACAAAGGCTGTAAAAATGGTTGGGGCTGATTCAATAGTGATGTTCTTGTACTACTTATGCGCCACCGTTTTGTCAACACCCATCGGTTTGTTGGCAGAGAGAGATTTGAGAGCTTGGACACTGCCCACTCGAATGGGACTCTTTGCTGTTATTTATGCGGGATTGATGGGTCAAGCATTTGGATCAATCGTCCACGCATGGGGTATGAGGATGAGAGGGCCTGTCTATGTCACAAGCTTTAGGCCATTGTCCATTATTATTGCAGCTGCCATGGCTTTTTTTCTTCTTGGTGATGCCCTCTATTTTGGAAGGTGA

Coding sequence (CDS)

ATGGCCAAAATGATTTGTACAATAGTGTCAATGATTGGAGCTTTTGTGGCTGTTCTCTATAAAGGCCCAATGGTTCTATCCCATCCATCTTCTTCCTCTCAAAATCTTCATCTTCTCCACCTTCACTCTTTCAACTCAGACTGGATTTTTGGTGGCTTCTTGTTTTCTCTTCAGTTCATCTGCTATGCCATTTGGTACATTCTTATGACAAAGGCTGTAAAAATGGTTGGGGCTGATTCAATAGTGATGTTCTTGTACTACTTATGCGCCACCGTTTTGTCAACACCCATCGGTTTGTTGGCAGAGAGAGATTTGAGAGCTTGGACACTGCCCACTCGAATGGGACTCTTTGCTGTTATTTATGCGGGATTGATGGGTCAAGCATTTGGATCAATCGTCCACGCATGGGGTATGAGGATGAGAGGGCCTGTCTATGTCACAAGCTTTAGGCCATTGTCCATTATTATTGCAGCTGCCATGGCTTTTTTTCTTCTTGGTGATGCCCTCTATTTTGGAAGGTGA

Protein sequence

MAKMICTIVSMIGAFVAVLYKGPMVLSHPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLFAVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFGR

Homology

[Close]

BLAST of MS027063 vs. NCBI nr
Match: XP_022150074.1 (WAT1-related protein At4g15540-like [Momordica charantia])

HSP 1 Score: 216.5 bits (550), Expect = 1.8e-52
Identity = 117/173 (67.63%), Postives = 140/173 (80.92%), Query Frame = 0


Query: 1   MAKMICTIVSMIGAFVAVLYKGPMVLSHPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQFI 60
           +AK+I TIVSMIGAFV VLYKGPM+L  P+S  + L LLH HS NSDW+ GGFLFS+Q++
Sbjct: 137 VAKIIGTIVSMIGAFVVVLYKGPMLL--PNSYKKPL-LLHHHS-NSDWVLGGFLFSIQYL 196

Query: 61  CYAIWYILM-TKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLFAV 120
           CYAI  +LM TK +K++GA+S V+  YY+C T+LSTPI LLAE D RAWTLPT M L AV
Sbjct: 197 CYAISSVLMTTKVIKVMGANSFVLLFYYICTTLLSTPICLLAETDFRAWTLPTDMALVAV 256

Query: 121 IYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           IYAG+MGQAF  I+H WG R + PVYV+SFRPLSI+IAA +AF LLGDALYFG
Sbjct: 257 IYAGMMGQAFYGIIHLWGFREKRPVYVSSFRPLSIVIAAVLAFILLGDALYFG 305

[Close]

BLAST of MS027063 vs. NCBI nr
Match: KAE8021576.1 (hypothetical protein FH972_007453 [Carpinus fangiana])

HSP 1 Score: 161.0 bits (406), Expect = 9.2e-36
Identity = 84/172 (48.84%), Postives = 120/172 (69.77%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLS-HPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQFI 61
           AK++ TIVS+ GA V VLYKGP ++S  P S S ++H   L S  ++WI GG L +++++
Sbjct: 122 AKVMGTIVSISGALVVVLYKGPTLISASPESPSPSVH-SPLGSSQTNWIIGGLLIAVEYL 181

Query: 62  CYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLFAVI 121
             +IWYIL T+ +K+  A+  V+FLY LCAT++S P+ LLAE +L AW L   + L A+I
Sbjct: 182 LISIWYILQTQIMKIYPAELTVVFLYNLCATIISAPVCLLAEANLSAWRLRPDITLVAII 241

Query: 122 YAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           Y+G  G    ++VH WG+ ++GPVY++ F+PLSI+IAAAM    LGDALY G
Sbjct: 242 YSGFAGSCLSAVVHTWGLHLKGPVYISIFKPLSIVIAAAMGVIFLGDALYLG 292

[Close]

BLAST of MS027063 vs. NCBI nr
Match: BAB10904.1 (nodulin-like protein [Arabidopsis thaliana])

HSP 1 Score: 159.1 bits (401), Expect = 3.5e-35
Identity = 82/175 (46.86%), Postives = 122/175 (69.71%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSH----PSSSSQNLHLLHLHSFNSDWIFGGFLFSL 61
           AK+I TIVS+ GA V +LYKGP VL+     P S + +L+  HL SF+S WI GG L + 
Sbjct: 122 AKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLY-QHLTSFDSSWIIGGLLLAT 181

Query: 62  QFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLF 121
           Q++  ++WYIL T+ +++   +  V+FLY LCAT++S P+ L AE+DL ++ L   + L 
Sbjct: 182 QYLLVSVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEKDLNSFILKPGVSLA 241

Query: 122 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +V+Y+G +  +FGS++H WG+ ++GPVY++ F+PLSI+IA AM    LGDALY G
Sbjct: 242 SVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVMFLGDALYLG 295

[Close]

BLAST of MS027063 vs. NCBI nr
Match: NP_198839.1 (nodulin MtN21 /EamA-like transporter family protein [Arabidopsis thaliana] >F4KHA8.1 RecName: Full=WAT1-related protein At5g40230 [Arabidopsis thaliana] >AED94522.1 nodulin MtN21 /EamA-like transporter family protein [Arabidopsis thaliana])

HSP 1 Score: 159.1 bits (401), Expect = 3.5e-35
Identity = 82/175 (46.86%), Postives = 122/175 (69.71%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSH----PSSSSQNLHLLHLHSFNSDWIFGGFLFSL 61
           AK+I TIVS+ GA V +LYKGP VL+     P S + +L+  HL SF+S WI GG L + 
Sbjct: 146 AKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLY-QHLTSFDSSWIIGGLLLAT 205

Query: 62  QFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLF 121
           Q++  ++WYIL T+ +++   +  V+FLY LCAT++S P+ L AE+DL ++ L   + L 
Sbjct: 206 QYLLVSVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEKDLNSFILKPGVSLA 265

Query: 122 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +V+Y+G +  +FGS++H WG+ ++GPVY++ F+PLSI+IA AM    LGDALY G
Sbjct: 266 SVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVMFLGDALYLG 319

[Close]

BLAST of MS027063 vs. NCBI nr
Match: KAG7543969.1 (EamA domain [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 159.1 bits (401), Expect = 3.5e-35
Identity = 83/175 (47.43%), Postives = 122/175 (69.71%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSH----PSSSSQNLHLLHLHSFNSDWIFGGFLFSL 61
           AK+I TIVS+ GA VAVLYKGP VL+     P S + +L+  HL SF+S WI GG L + 
Sbjct: 171 AKIIGTIVSISGALVAVLYKGPKVLTDASLTPPSPTISLY-QHLTSFDSSWIIGGLLLAT 230

Query: 62  QFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLF 121
           Q++  ++WYIL T+ +++   +  V+FLY LC T++S P+ L AE+DL ++ L   + L 
Sbjct: 231 QYLLVSVWYILQTRVMELYPEEITVVFLYNLCGTLISAPVCLFAEKDLTSFILKPGVSLA 290

Query: 122 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +V+Y+G +  +FGS++H WG+ ++GPVY++ F+PLSI+IA AM    LGDALY G
Sbjct: 291 SVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVLFLGDALYLG 344

[Close]

BLAST of MS027063 vs. ExPASy Swiss-Prot
Match: F4KHA8 (WAT1-related protein At5g40230 OS=Arabidopsis thaliana OX=3702 GN=At5g40230 PE=3 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 4.6e-38
Identity = 82/175 (46.86%), Postives = 122/175 (69.71%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSH----PSSSSQNLHLLHLHSFNSDWIFGGFLFSL 61
           AK+I TIVS+ GA V +LYKGP VL+     P S + +L+  HL SF+S WI GG L + 
Sbjct: 146 AKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLY-QHLTSFDSSWIIGGLLLAT 205

Query: 62  QFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLF 121
           Q++  ++WYIL T+ +++   +  V+FLY LCAT++S P+ L AE+DL ++ L   + L 
Sbjct: 206 QYLLVSVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEKDLNSFILKPGVSLA 265

Query: 122 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +V+Y+G +  +FGS++H WG+ ++GPVY++ F+PLSI+IA AM    LGDALY G
Sbjct: 266 SVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVMFLGDALYLG 319

[Close]

BLAST of MS027063 vs. ExPASy Swiss-Prot
Match: F4JK59 (WAT1-related protein At4g15540 OS=Arabidopsis thaliana OX=3702 GN=At4g15540 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 6.9e-34
Identity = 78/171 (45.61%), Postives = 108/171 (63.16%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSHPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQFIC 61
           AK+I TIVS+ GA V VLYKGP +L   S +          SF S WI GG L  LQF+ 
Sbjct: 142 AKIIGTIVSISGALVIVLYKGPKLLVAASFT----------SFESSWIIGGLLLGLQFLL 201

Query: 62  YAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLFAVIY 121
            ++W+IL T  +++   +  V+F Y LCAT++S  + LL E+DL +W L     L +VIY
Sbjct: 202 LSVWFILQTHIMEIYPEEIAVVFCYNLCATLISGTVCLLVEKDLNSWQLKPGFSLASVIY 261

Query: 122 AGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +GL   + GS++H WG+ ++GPVY++ F+PLSI IA AMA   LGD L+ G
Sbjct: 262 SGLFDTSLGSVIHTWGLHVKGPVYISLFKPLSIAIAVAMAAIFLGDTLHLG 302

[Close]

BLAST of MS027063 vs. ExPASy Swiss-Prot
Match: Q9FL08 (WAT1-related protein At5g40240 OS=Arabidopsis thaliana OX=3702 GN=At5g40240 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 6.4e-32
Identity = 76/175 (43.43%), Postives = 109/175 (62.29%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSHPSSS----SQNLHLLHLHSFNSDWIFGGFLFSL 61
           AK+I  I+S+ GA V VLYKGP VL+  S +    +  LH   L S  S WI GG L + 
Sbjct: 145 AKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLH-QQLTSIESSWIIGGLLLAS 204

Query: 62  QFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLF 121
           Q+   ++WYIL T+ +++   +  V+F Y L AT++S P+ L AE +L +W L   + L 
Sbjct: 205 QYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLTSWVLKPDISLA 264

Query: 122 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           A+IY+G+    F ++ H WG+ ++GPVY++ FRPLSI IA AM    LGDAL+ G
Sbjct: 265 AIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLGDALHLG 318

[Close]

BLAST of MS027063 vs. ExPASy Swiss-Prot
Match: Q56X95 (WAT1-related protein At3g28130 OS=Arabidopsis thaliana OX=3702 GN=At3g28130 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.9e-24
Identity = 69/175 (39.43%), Postives = 103/175 (58.86%), Query Frame = 0


Query: 1   MAKMICTIVSMIGAFVAVLYKGPMVLSHPSSSSQNLH--LLHLHSFNSDWIFGGFLFSLQ 60
           +AKM+ TIVS++GA V VLY GP V +  S     L   LL L S NSDWI GG L +++
Sbjct: 137 VAKMVGTIVSLVGALVVVLYHGPRVFTPSSPPFPQLRQLLLPLSSSNSDWIIGGCLLAIK 196

Query: 61  FICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRA-WTLPTRMGLF 120
                + +IL    +K+  A   V F Y+L A++L++ IG++AE++  + W +   + L 
Sbjct: 197 DTLVPVAFILQAHIMKLYPAPFTVSFFYFLIASILTSLIGIVAEKNNPSIWIIHFDITLV 256

Query: 121 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
            ++  G+    + +I H W +R +GPVY+  FRPLSI+IA  M    LGD+ Y G
Sbjct: 257 CIVVGGIFNPGYYAI-HLWAVRNKGPVYLAIFRPLSILIAVIMGAIFLGDSFYLG 310

[Close]

BLAST of MS027063 vs. ExPASy Swiss-Prot
Match: Q94JU2 (WAT1-related protein At3g28050 OS=Arabidopsis thaliana OX=3702 GN=At3g28050 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-23
Identity = 62/175 (35.43%), Postives = 100/175 (57.14%), Query Frame = 0


Query: 1   MAKMICTIVSMIGAFVAVLYKGPMVL--SHPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQ 60
           +AKM+ T+VS+ GAF+  LY GP+V+  S PS S      L   S N +WI G    +++
Sbjct: 136 VAKMLGTVVSIGGAFIVTLYNGPVVIAKSPPSVS------LRSQSTNPNWILGAGFLAVE 195

Query: 61  FICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAE-RDLRAWTLPTRMGLF 120
           + C  +WYI+ T+ ++   A+  V+  Y +  +  +  + L  E  DL AW +   + L 
Sbjct: 196 YFCVPLWYIVQTQIMREYPAEFTVVCFYSIGVSFWTALVTLFTEGNDLGAWKIKPNIALV 255

Query: 121 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +++ +GL G    + +H W +R++GP++V  F+PLSI IA AM    L D+LY G
Sbjct: 256 SIVCSGLFGSCINNTIHTWALRIKGPLFVAMFKPLSIAIAVAMGVIFLRDSLYIG 304

[Close]

BLAST of MS027063 vs. ExPASy TrEMBL
Match: A0A6J1D8Y3 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111018340 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 8.9e-53
Identity = 117/173 (67.63%), Postives = 140/173 (80.92%), Query Frame = 0


Query: 1   MAKMICTIVSMIGAFVAVLYKGPMVLSHPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQFI 60
           +AK+I TIVSMIGAFV VLYKGPM+L  P+S  + L LLH HS NSDW+ GGFLFS+Q++
Sbjct: 137 VAKIIGTIVSMIGAFVVVLYKGPMLL--PNSYKKPL-LLHHHS-NSDWVLGGFLFSIQYL 196

Query: 61  CYAIWYILM-TKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLFAV 120
           CYAI  +LM TK +K++GA+S V+  YY+C T+LSTPI LLAE D RAWTLPT M L AV
Sbjct: 197 CYAISSVLMTTKVIKVMGANSFVLLFYYICTTLLSTPICLLAETDFRAWTLPTDMALVAV 256

Query: 121 IYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           IYAG+MGQAF  I+H WG R + PVYV+SFRPLSI+IAA +AF LLGDALYFG
Sbjct: 257 IYAGMMGQAFYGIIHLWGFREKRPVYVSSFRPLSIVIAAVLAFILLGDALYFG 305

[Close]

BLAST of MS027063 vs. ExPASy TrEMBL
Match: A0A7N0T2G2 (WAT1-related protein OS=Kalanchoe fedtschenkoi OX=63787 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 4.5e-36
Identity = 82/171 (47.95%), Postives = 117/171 (68.42%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSHPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQFIC 61
           AK+  T++S+ GA   VLYKGP+VLS  ++S  +LH L       +WI GG L + +++ 
Sbjct: 139 AKLCGTLLSIAGALTVVLYKGPIVLS--TTSRFSLHQL----LQKNWIIGGTLLATEYLL 198

Query: 62  YAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLFAVIY 121
            AIWYI+ TK +++  ++ +V+ LY +CA VLS P+ L  E DL AW L   +GL A++Y
Sbjct: 199 LAIWYIIQTKIIQLYPSEPLVVLLYNVCAMVLSIPVSLYGEPDLSAWKLRADVGLAAIVY 258

Query: 122 AGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +G  G  F ++VH WG+R++GPVY++ FRPLSIIIAA+M FF LGD LY G
Sbjct: 259 SGCFGSVFSTVVHTWGLRIKGPVYISVFRPLSIIIAASMGFFFLGDNLYLG 303

[Close]

BLAST of MS027063 vs. ExPASy TrEMBL
Match: A0A5N6QVQ8 (WAT1-related protein OS=Carpinus fangiana OX=176857 GN=FH972_007453 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 4.5e-36
Identity = 84/172 (48.84%), Postives = 120/172 (69.77%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLS-HPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQFI 61
           AK++ TIVS+ GA V VLYKGP ++S  P S S ++H   L S  ++WI GG L +++++
Sbjct: 122 AKVMGTIVSISGALVVVLYKGPTLISASPESPSPSVH-SPLGSSQTNWIIGGLLIAVEYL 181

Query: 62  CYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLFAVI 121
             +IWYIL T+ +K+  A+  V+FLY LCAT++S P+ LLAE +L AW L   + L A+I
Sbjct: 182 LISIWYILQTQIMKIYPAELTVVFLYNLCATIISAPVCLLAEANLSAWRLRPDITLVAII 241

Query: 122 YAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           Y+G  G    ++VH WG+ ++GPVY++ F+PLSI+IAAAM    LGDALY G
Sbjct: 242 YSGFAGSCLSAVVHTWGLHLKGPVYISIFKPLSIVIAAAMGVIFLGDALYLG 292

[Close]

BLAST of MS027063 vs. ExPASy TrEMBL
Match: R0GQ83 (WAT1-related protein OS=Capsella rubella OX=81985 GN=CARUB_v10007676mg PE=3 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.7e-35
Identity = 82/171 (47.95%), Postives = 117/171 (68.42%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSHPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQFIC 61
           AK+I TIVS+ GA V VLYKGP VLS  S +       HL SF+S WI GG L + Q++ 
Sbjct: 146 AKIIGTIVSISGALVVVLYKGPKVLSDASLTPT---YQHLTSFDSSWIIGGLLLATQYLL 205

Query: 62  YAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLFAVIY 121
            ++WYIL T+ +++   +  V+FLY LCAT++S P+ L AE+DL ++ L   + L +V+Y
Sbjct: 206 ISVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEKDLTSFMLKPGVSLASVMY 265

Query: 122 AGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +G +  +FGS++H WG+ ++GPVY+  F+PLSI+IA AM    LGDALY G
Sbjct: 266 SGGLVSSFGSVIHTWGLHLKGPVYIALFKPLSIVIAVAMGVIFLGDALYLG 313

[Close]

BLAST of MS027063 vs. ExPASy TrEMBL
Match: A0A178UMT4 (WAT1-related protein OS=Arabidopsis thaliana OX=3702 GN=AXX17_At5g37880 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 2.2e-35
Identity = 82/175 (46.86%), Postives = 122/175 (69.71%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSH----PSSSSQNLHLLHLHSFNSDWIFGGFLFSL 61
           AK+I TIVS+ GA V +LYKGP VL+     P S + +L+  HL SF+S WI GG L + 
Sbjct: 146 AKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLY-QHLTSFDSSWIIGGLLLAT 205

Query: 62  QFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLF 121
           Q++  ++WYIL T+ +++   +  V+FLY LCAT++S P+ L AE+DL ++ L   + L 
Sbjct: 206 QYLLVSVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEKDLTSFILKPGVSLA 265

Query: 122 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +V+Y+G +  +FGS++H WG+ ++GPVY++ F+PLSI+IA AM    LGDALY G
Sbjct: 266 SVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVMFLGDALYLG 319

[Close]

BLAST of MS027063 vs. TAIR 10
Match: AT5G40230.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 159.1 bits (401), Expect = 3.3e-39
Identity = 82/175 (46.86%), Postives = 122/175 (69.71%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSH----PSSSSQNLHLLHLHSFNSDWIFGGFLFSL 61
           AK+I TIVS+ GA V +LYKGP VL+     P S + +L+  HL SF+S WI GG L + 
Sbjct: 146 AKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLY-QHLTSFDSSWIIGGLLLAT 205

Query: 62  QFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLF 121
           Q++  ++WYIL T+ +++   +  V+FLY LCAT++S P+ L AE+DL ++ L   + L 
Sbjct: 206 QYLLVSVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEKDLNSFILKPGVSLA 265

Query: 122 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +V+Y+G +  +FGS++H WG+ ++GPVY++ F+PLSI+IA AM    LGDALY G
Sbjct: 266 SVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVMFLGDALYLG 319

[Close]

BLAST of MS027063 vs. TAIR 10
Match: AT4G15540.1 (EamA-like transporter family )

HSP 1 Score: 145.2 bits (365), Expect = 4.9e-35
Identity = 78/171 (45.61%), Postives = 108/171 (63.16%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSHPSSSSQNLHLLHLHSFNSDWIFGGFLFSLQFIC 61
           AK+I TIVS+ GA V VLYKGP +L   S +          SF S WI GG L  LQF+ 
Sbjct: 142 AKIIGTIVSISGALVIVLYKGPKLLVAASFT----------SFESSWIIGGLLLGLQFLL 201

Query: 62  YAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLFAVIY 121
            ++W+IL T  +++   +  V+F Y LCAT++S  + LL E+DL +W L     L +VIY
Sbjct: 202 LSVWFILQTHIMEIYPEEIAVVFCYNLCATLISGTVCLLVEKDLNSWQLKPGFSLASVIY 261

Query: 122 AGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           +GL   + GS++H WG+ ++GPVY++ F+PLSI IA AMA   LGD L+ G
Sbjct: 262 SGLFDTSLGSVIHTWGLHVKGPVYISLFKPLSIAIAVAMAAIFLGDTLHLG 302

[Close]

BLAST of MS027063 vs. TAIR 10
Match: AT5G40240.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 138.7 bits (348), Expect = 4.6e-33
Identity = 76/175 (43.43%), Postives = 109/175 (62.29%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSHPSSS----SQNLHLLHLHSFNSDWIFGGFLFSL 61
           AK+I  I+S+ GA V VLYKGP VL+  S +    +  LH   L S  S WI GG L + 
Sbjct: 145 AKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLH-QQLTSIESSWIIGGLLLAS 204

Query: 62  QFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLF 121
           Q+   ++WYIL T+ +++   +  V+F Y L AT++S P+ L AE +L +W L   + L 
Sbjct: 205 QYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLTSWVLKPDISLA 264

Query: 122 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           A+IY+G+    F ++ H WG+ ++GPVY++ FRPLSI IA AM    LGDAL+ G
Sbjct: 265 AIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLGDALHLG 318

[Close]

BLAST of MS027063 vs. TAIR 10
Match: AT5G40240.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 138.7 bits (348), Expect = 4.6e-33
Identity = 76/175 (43.43%), Postives = 109/175 (62.29%), Query Frame = 0


Query: 2   AKMICTIVSMIGAFVAVLYKGPMVLSHPSSS----SQNLHLLHLHSFNSDWIFGGFLFSL 61
           AK+I  I+S+ GA V VLYKGP VL+  S +    +  LH   L S  S WI GG L + 
Sbjct: 159 AKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLH-QQLTSIESSWIIGGLLLAS 218

Query: 62  QFICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRAWTLPTRMGLF 121
           Q+   ++WYIL T+ +++   +  V+F Y L AT++S P+ L AE +L +W L   + L 
Sbjct: 219 QYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLTSWVLKPDISLA 278

Query: 122 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
           A+IY+G+    F ++ H WG+ ++GPVY++ FRPLSI IA AM    LGDAL+ G
Sbjct: 279 AIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLGDALHLG 332

[Close]

BLAST of MS027063 vs. TAIR 10
Match: AT3G28130.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 113.2 bits (282), Expect = 2.1e-25
Identity = 69/175 (39.43%), Postives = 103/175 (58.86%), Query Frame = 0


Query: 1   MAKMICTIVSMIGAFVAVLYKGPMVLSHPSSSSQNLH--LLHLHSFNSDWIFGGFLFSLQ 60
           +AKM+ TIVS++GA V VLY GP V +  S     L   LL L S NSDWI GG L +++
Sbjct: 51  VAKMVGTIVSLVGALVVVLYHGPRVFTPSSPPFPQLRQLLLPLSSSNSDWIIGGCLLAIK 110

Query: 61  FICYAIWYILMTKAVKMVGADSIVMFLYYLCATVLSTPIGLLAERDLRA-WTLPTRMGLF 120
                + +IL    +K+  A   V F Y+L A++L++ IG++AE++  + W +   + L 
Sbjct: 111 DTLVPVAFILQAHIMKLYPAPFTVSFFYFLIASILTSLIGIVAEKNNPSIWIIHFDITLV 170

Query: 121 AVIYAGLMGQAFGSIVHAWGMRMRGPVYVTSFRPLSIIIAAAMAFFLLGDALYFG 173
            ++  G+    + +I H W +R +GPVY+  FRPLSI+IA  M    LGD+ Y G
Sbjct: 171 CIVVGGIFNPGYYAI-HLWAVRNKGPVYLAIFRPLSILIAVIMGAIFLGDSFYLG 224

The following BLAST results are available for this feature:

BLAST of MS027063 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150074.1	1.8e-52	67.63	WAT1-related protein At4g15540-like [Momordica charantia]	[more]
KAE8021576.1	9.2e-36	48.84	hypothetical protein FH972_007453 [Carpinus fangiana]	[more]
BAB10904.1	3.5e-35	46.86	nodulin-like protein [Arabidopsis thaliana]	[more]
NP_198839.1	3.5e-35	46.86	nodulin MtN21 /EamA-like transporter family protein [Arabidopsis thaliana] >F4KH...	[more]
KAG7543969.1	3.5e-35	47.43	EamA domain [Arabidopsis thaliana x Arabidopsis arenosa]	[more]

BLAST of MS027063 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KHA8	4.6e-38	46.86	WAT1-related protein At5g40230 OS=Arabidopsis thaliana OX=3702 GN=At5g40230 PE=3...	[more]
F4JK59	6.9e-34	45.61	WAT1-related protein At4g15540 OS=Arabidopsis thaliana OX=3702 GN=At4g15540 PE=2...	[more]
Q9FL08	6.4e-32	43.43	WAT1-related protein At5g40240 OS=Arabidopsis thaliana OX=3702 GN=At5g40240 PE=2...	[more]
Q56X95	2.9e-24	39.43	WAT1-related protein At3g28130 OS=Arabidopsis thaliana OX=3702 GN=At3g28130 PE=2...	[more]
Q94JU2	1.4e-23	35.43	WAT1-related protein At3g28050 OS=Arabidopsis thaliana OX=3702 GN=At3g28050 PE=2...	[more]

BLAST of MS027063 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D8Y3	8.9e-53	67.63	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111018340 PE=3 SV=1	[more]
A0A7N0T2G2	4.5e-36	47.95	WAT1-related protein OS=Kalanchoe fedtschenkoi OX=63787 PE=3 SV=1	[more]
A0A5N6QVQ8	4.5e-36	48.84	WAT1-related protein OS=Carpinus fangiana OX=176857 GN=FH972_007453 PE=3 SV=1	[more]
R0GQ83	1.7e-35	47.95	WAT1-related protein OS=Capsella rubella OX=81985 GN=CARUB_v10007676mg PE=3 SV=1	[more]
A0A178UMT4	2.2e-35	46.86	WAT1-related protein OS=Arabidopsis thaliana OX=3702 GN=AXX17_At5g37880 PE=3 SV=...	[more]

BLAST of MS027063 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G40230.1	3.3e-39	46.86	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G15540.1	4.9e-35	45.61	EamA-like transporter family	[more]
AT5G40240.1	4.6e-33	43.43	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G40240.2	4.6e-33	43.43	nodulin MtN21 /EamA-like transporter family protein	[more]
AT3G28130.1	2.1e-25	39.43	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 51..170 e-value: 1.6E-6 score: 28.4
None	No IPR available	PANTHER	PTHR31218:SF48	WAT1-RELATED PROTEIN	coord: 1..172
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 1..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS027063.1	MS027063.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity