MS026607 (gene) Bitter gourd (TR) v1

Overview
NameMS026607
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionbeta-amyrin 11-oxidase-like
Locationscaffold1315: 291994 .. 292982 (-)
RNA-Seq ExpressionMS026607
SyntenyMS026607
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTCAAGAATTGGTTGGTATTGATTCTTGCAGCCTTTGTTTTTGTTTTTGGAGTTTTGAAGAGATTGAATGGTTGGTATTATGGGGCCAGGCTGAGGAACATATGGGCTGAGCTTCCTCCCGGTGACATGAACTGGCCTCTTCTCGGTTCTACTCTATCCTTCGTCAAAGATGTCAGTAATGGTCGACCTCAAAATTTCGTTCACACCTTTTTCAACAGGTCTGTCTCATCAATACTCATTCTTGATTTATTGAAGATAATAAAATACTCATTCTTGGTCGAATTAACTAGTTGAGCTCTCACAATTACAGTGATATTAATAAAGTATTAAATTTATCATAACATATCAATTTGAGATTTTGGGTTCAGTGGTAGTTTAACATAGTATAAAAGTATATGATTCTATATTCAGACGAGTCGTTTCTCATTGATTTATATTATTATAGATATGGGAAAGTTGATATGTTCAAAACTCACGTACTTGGGAGAGCAAGTATAATCGTGTGCACACCTGAACTCTGCCGTCAAGTTTTAGGAGATGAAGAAAAATTTGTGCCTAGTTTACCCGTAAACATAAAATTGTTATCGGGTAAAAAGTCACTGATCCAATTTTCGAAGGCAGAGCACCGGCGGCTCCGACGACTGATGACGGCGCCGATCAGCGGCCACGCGGCGCTGGAGATGTACATCTGCCATATTGAAGAGACAGTGGTGGGTGGGTTGGAGGAGTGGGAGAGTATGCAGAAGCCAATAGAACTGTTGACAGAGATAAAAAAGCTAACTTTCAAAGTTATTTGGAATATTTTCATGGGCTCAACCACCACAGACTATTCCATTGGAGAAATGGAGGCTTTGTTTTATGATGTTGCATTAGGGTTCATGTCTTTGCCCATCAACTTTCCTGGCTTCTCCTTCTATAAATCGTTCAAGGTAAGCCGCTTTTATCGTTTTTTAATTATACTCTTCTCGTCGGTGAATATTTAA

mRNA sequence

ATGGAGTTCAAGAATTGGTTGGTATTGATTCTTGCAGCCTTTGTTTTTGTTTTTGGAGTTTTGAAGAGATTGAATGGTTGGTATTATGGGGCCAGGCTGAGGAACATATGGGCTGAGCTTCCTCCCGGTGACATGAACTGGCCTCTTCTCGGTTCTACTCTATCCTTCGTCAAAGATGTCAGTAATGGTCGACCTCAAAATTTCGTTCACACCTTTTTCAACAGATATGGGAAAGTTGATATGTTCAAAACTCACGTACTTGGGAGAGCAAGTATAATCGTGTGCACACCTGAACTCTGCCGTCAAGTTTTAGGAGATGAAGAAAAATTTGTGCCTAGTTTACCCGTAAACATAAAATTGTTATCGGGTAAAAAGTCACTGATCCAATTTTCGAAGGCAGAGCACCGGCGGCTCCGACGACTGATGACGGCGCCGATCAGCGGCCACGCGGCGCTGGAGATGTACATCTGCCATATTGAAGAGACAGTGGTGGGTGGGTTGGAGGAGTGGGAGAGTATGCAGAAGCCAATAGAACTGTTGACAGAGATAAAAAAGCTAACTTTCAAAGTTATTTGGAATATTTTCATGGGCTCAACCACCACAGACTATTCCATTGGAGAAATGGAGGCTTTGTTTTATGATGTTGCATTAGGGTTCATGTCTTTGCCCATCAACTTTCCTGGCTTCTCCTTCTATAAATCGTTCAAGGTAAGCCGCTTTTATCGTTTTTTAATTATACTCTTCTCGTCGGTGAATATTTAA

Coding sequence (CDS)

ATGGAGTTCAAGAATTGGTTGGTATTGATTCTTGCAGCCTTTGTTTTTGTTTTTGGAGTTTTGAAGAGATTGAATGGTTGGTATTATGGGGCCAGGCTGAGGAACATATGGGCTGAGCTTCCTCCCGGTGACATGAACTGGCCTCTTCTCGGTTCTACTCTATCCTTCGTCAAAGATGTCAGTAATGGTCGACCTCAAAATTTCGTTCACACCTTTTTCAACAGATATGGGAAAGTTGATATGTTCAAAACTCACGTACTTGGGAGAGCAAGTATAATCGTGTGCACACCTGAACTCTGCCGTCAAGTTTTAGGAGATGAAGAAAAATTTGTGCCTAGTTTACCCGTAAACATAAAATTGTTATCGGGTAAAAAGTCACTGATCCAATTTTCGAAGGCAGAGCACCGGCGGCTCCGACGACTGATGACGGCGCCGATCAGCGGCCACGCGGCGCTGGAGATGTACATCTGCCATATTGAAGAGACAGTGGTGGGTGGGTTGGAGGAGTGGGAGAGTATGCAGAAGCCAATAGAACTGTTGACAGAGATAAAAAAGCTAACTTTCAAAGTTATTTGGAATATTTTCATGGGCTCAACCACCACAGACTATTCCATTGGAGAAATGGAGGCTTTGTTTTATGATGTTGCATTAGGGTTCATGTCTTTGCCCATCAACTTTCCTGGCTTCTCCTTCTATAAATCGTTCAAGGTAAGCCGCTTTTATCGTTTTTTAATTATACTCTTCTCGTCGGTGAATATTTAA

Protein sequence

MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDVSNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKLLSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELLTEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRFYRFLIILFSSVNI
Homology
BLAST of MS026607 vs. NCBI nr
Match: XP_022145285.1 (beta-amyrin 11-oxidase-like [Momordica charantia])

HSP 1 Score: 479.9 bits (1234), Expect = 1.3e-131
Identity = 232/239 (97.07%), Postives = 236/239 (98.74%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDM+WPLLGSTLSFVKDV
Sbjct: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMSWPLLGSTLSFVKDV 60

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
           SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL
Sbjct: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           LSGKKSLIQFSKAEHRRLRRLMTAPISGH ALEMYICHIEETVVGGLEEWESMQ+PIELL
Sbjct: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHTALEMYICHIEETVVGGLEEWESMQRPIELL 180

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSR 240
           TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALF+DV+LGFMSLPINFPGFSFYKSFK  R
Sbjct: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFHDVSLGFMSLPINFPGFSFYKSFKARR 239

BLAST of MS026607 vs. NCBI nr
Match: XP_023007796.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 358.6 bits (919), Expect = 4.4e-95
Identity = 171/252 (67.86%), Postives = 209/252 (82.94%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           ME KNWLVLI+AAFVFV G+LKRLNGWYY A+L   W +LPPGDM WPLLGST+S++K+ 
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNF 63

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
            +G+P NF+  F+ RYGK DM+KTH+LGRA+IIVC+ ELCRQVLGDE+ F PSLP NI +
Sbjct: 64  ISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           L+G+KSL+Q  KAEH+RLRRL  APISGHAALEMYI HIE TV+ GLEEW SM+ PIELL
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRF 240
           +EIKKLTFK+IWNIFMGS++ + SIGEME LF +VALGF+SLPINFPGF+ +K+ K  + 
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARK- 243

Query: 241 YRFLIILFSSVN 253
            + L IL S ++
Sbjct: 244 -KLLDILQSIID 253

BLAST of MS026607 vs. NCBI nr
Match: XP_023552542.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 357.1 bits (915), Expect = 1.3e-94
Identity = 170/252 (67.46%), Postives = 208/252 (82.54%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           ME KNWLVLI+AAFVFV G+LKR+NGWYY A+L   W +LPPG M WPLLGST+S++K+ 
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWPKLPPGHMGWPLLGSTISYIKNF 63

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
            +G+P NF+  F+ RYGK DM+KTH+LGRA+IIVC+ ELCRQVLGDE+ F PSLP NI +
Sbjct: 64  ISGQPSNFMANFYIRYGKSDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           L+G+KSL+Q  KAEH+RLRRL  APISGHAALEMYI HIE TV+ GLEEW SM+ PIELL
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRF 240
           +EIKKLTFK+IWNIFMGS++ + SIGEME LF +VALGF+SLPINFPGF+ +K+ K  + 
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARK- 243

Query: 241 YRFLIILFSSVN 253
            + L IL S +N
Sbjct: 244 -KLLDILQSIIN 253

BLAST of MS026607 vs. NCBI nr
Match: XP_022923277.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 356.7 bits (914), Expect = 1.7e-94
Identity = 174/252 (69.05%), Postives = 205/252 (81.35%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           ME KNWLVL++AAFVFV GVLKRLNGWYY A+L   W +LPPGDM WPLLGST+ F+K  
Sbjct: 4   MEVKNWLVLVVAAFVFVLGVLKRLNGWYYAAKLGKAWPKLPPGDMGWPLLGSTILFLKSF 63

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
           S G+P+NF+   F RYGK DM+KTH+LGRA+IIVCT ELCRQVLGDE  F PS PV+I  
Sbjct: 64  SCGQPRNFISNIFIRYGKGDMYKTHILGRATIIVCTSELCRQVLGDEHNFKPSFPVSINR 123

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           LSG+KSLIQ  KAEHRRLRRL  APISGHAAL+MYI HIE TV+ GLEEW SM+ PIELL
Sbjct: 124 LSGRKSLIQVFKAEHRRLRRLTMAPISGHAALKMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRF 240
           +E+KKLTFK+IWNIFMGS++ +YSI EM  LF +VALGF+SLPINFPGF+F+KS K  + 
Sbjct: 184 SEMKKLTFKIIWNIFMGSSSFEYSIDEMVVLFSEVALGFISLPINFPGFNFHKSLKARK- 243

Query: 241 YRFLIILFSSVN 253
            + L IL S ++
Sbjct: 244 -KLLKILQSIIH 253

BLAST of MS026607 vs. NCBI nr
Match: KAG6577627.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 356.7 bits (914), Expect = 1.7e-94
Identity = 174/252 (69.05%), Postives = 205/252 (81.35%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           ME KNWLVL++AAFVFV GVLKRLNGWYY A+L   W +LPPGDM WPLLGST+ F+K  
Sbjct: 4   MEVKNWLVLVVAAFVFVLGVLKRLNGWYYAAKLGKAWPKLPPGDMGWPLLGSTILFLKSF 63

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
           S G+P+NF+   F RYGK DM+KTH+LGRA+IIVCT ELCRQVLGDE  F PS PV+I  
Sbjct: 64  SCGQPRNFISNIFIRYGKGDMYKTHILGRATIIVCTSELCRQVLGDEHNFKPSFPVSINR 123

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           LSG+KSLIQ  KAEHRRLRRL  APISGHAAL+MYI HIE TV+ GLEEW SM+ PIELL
Sbjct: 124 LSGRKSLIQVFKAEHRRLRRLTMAPISGHAALKMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRF 240
           +E+KKLTFK+IWNIFMGS++ +YSI EM  LF +VALGF+SLPINFPGF+F+KS K  + 
Sbjct: 184 SEMKKLTFKIIWNIFMGSSSFEYSIDEMVVLFSEVALGFISLPINFPGFNFHKSLKARK- 243

Query: 241 YRFLIILFSSVN 253
            + L IL S ++
Sbjct: 244 -KLLKILQSIIH 253

BLAST of MS026607 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.4e-56
Identity = 105/230 (45.65%), Postives = 153/230 (66.52%), Query Frame = 0

Query: 10  ILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDVSNGRPQNFV 69
           +L  ++F    ++ LNGWYY  +LR     LPPGDM WPL+G  LSF+KD S+G P +F+
Sbjct: 13  LLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFI 72

Query: 70  HTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKLLSGKKSLIQ 129
           +    +YG+  ++KTH+ G  SIIVC P++CR+VL D+  F    P +IK L+  + +I 
Sbjct: 73  NNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMID 132

Query: 130 FSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELLTEIKKLTFK 189
            S AEHR  RRL+T+PI GH AL MY+  +EE V+  LEE  SM+ P+ELL E+KK++FK
Sbjct: 133 VSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFK 192

Query: 190 VIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSR 240
            I ++FMGS+  D  I ++ + F D+  G  S+PIN PGF+F+K+ +  +
Sbjct: 193 AIVHVFMGSSNQDI-IKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARK 241

BLAST of MS026607 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 178.7 bits (452), Expect = 8.2e-44
Identity = 92/246 (37.40%), Postives = 150/246 (60.98%), Query Frame = 0

Query: 6   WLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDVSNGRP 65
           W  LI+     +  VLKR+N W Y ++L      LPPGD+ WP++G+  SF++      P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 66  QNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKLLSGKK 125
           ++F+ ++  RYG+  ++K H+ G   ++V TPE CR+VL D++ F    P +   L G+K
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 126 SLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELLTEIKK 185
           S +  S  EH+RLRRL +AP++G  AL +YI  IEETV   LE+W  M + IE L+ ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 189

Query: 186 LTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRFYRFLI 245
           LTFKVI  IF+ S+ +++ +  +E  + ++  G  ++ IN PGF+++++ K     + L+
Sbjct: 190 LTFKVIMYIFL-SSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKAR---KKLV 249

Query: 246 ILFSSV 252
             F S+
Sbjct: 250 AAFQSI 250

BLAST of MS026607 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.2e-41
Identity = 87/246 (35.37%), Postives = 143/246 (58.13%), Query Frame = 0

Query: 6   WLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDVSNGRP 65
           W  L++     +  +++++N W Y + L      LPPGD+ WP +G+ LSF++      P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 66  QNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKLLSGKK 125
            +F  T   RYG   ++K H+ G  SIIV T + CR+VL D++ F P  P +   L G+K
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 126 SLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELLTEIKK 185
           S +  S  EH+RLRRL  AP++GH AL  YI +IEE V+  L++W  M +  E LT ++K
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRK 190

Query: 186 LTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRFYRFLI 245
           LTF++I  IF+ S+ ++  +  +E  +  +  G  ++ +N PGF+++++ K     + L+
Sbjct: 191 LTFRIIMYIFL-SSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKAR---KTLV 250

Query: 246 ILFSSV 252
             F S+
Sbjct: 251 AAFQSI 251

BLAST of MS026607 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.9e-41
Identity = 85/232 (36.64%), Postives = 137/232 (59.05%), Query Frame = 0

Query: 10  ILAAFVFVFGVLKRLNGWYYGARL-RNIWAELPPGDMNWPLLGSTLSFVKDVSNGRPQNF 69
           ++A  V V  V++R + W   A L     + LPPG+M WP++GS  +F++   +G P  F
Sbjct: 24  VVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNPDAF 83

Query: 70  VHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKLLSGKKSLI 129
           + +F  R+G+  +++T +    +I+  TPE C+QVL D+E FV   P     L G KS +
Sbjct: 84  IASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFV 143

Query: 130 QFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQK-PIELLTEIKKLT 189
             S  +HRR+R+L  API+G  AL  Y+  I++TVV  L  W S +   +E LTE++++T
Sbjct: 144 NMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMT 203

Query: 190 FKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSR 240
           FK+I  IFM S   D ++  +E  + D+  G  ++ IN PGF++Y++ +  R
Sbjct: 204 FKIIVQIFM-SGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARR 254

BLAST of MS026607 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 6.7e-38
Identity = 81/235 (34.47%), Postives = 132/235 (56.17%), Query Frame = 0

Query: 8   VLILAAFVFVFGVLKRLNGWYYGARL-RNIWAELPPGDMNWPLLGSTLSFVKDVSNGRPQ 67
           V+   A V +   ++  + WY+ A L       LPPGDM WPL+G   +F++   +GRP 
Sbjct: 12  VVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPD 71

Query: 68  NFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKLLSGKKS 127
           +F+ +F  R+G+  +++  +    +I+  TPE C+QVL D++ FV   P     L G KS
Sbjct: 72  SFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKS 131

Query: 128 LIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESM--QKPIELLTEIK 187
            +     EHRRLR+L  API+G  AL  Y+  I++TVV  L  W         E LTE++
Sbjct: 132 FVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELR 191

Query: 188 KLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSR 240
           ++TF++I  IFMG    + +  E+E  + ++  G  ++ I+ PGF+++K+ +  R
Sbjct: 192 RMTFRIIVQIFMGG-ADERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRARR 245

BLAST of MS026607 vs. ExPASy TrEMBL
Match: A0A6J1CU16 (beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111014773 PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 6.3e-132
Identity = 232/239 (97.07%), Postives = 236/239 (98.74%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDM+WPLLGSTLSFVKDV
Sbjct: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMSWPLLGSTLSFVKDV 60

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
           SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL
Sbjct: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           LSGKKSLIQFSKAEHRRLRRLMTAPISGH ALEMYICHIEETVVGGLEEWESMQ+PIELL
Sbjct: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHTALEMYICHIEETVVGGLEEWESMQRPIELL 180

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSR 240
           TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALF+DV+LGFMSLPINFPGFSFYKSFK  R
Sbjct: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFHDVSLGFMSLPINFPGFSFYKSFKARR 239

BLAST of MS026607 vs. ExPASy TrEMBL
Match: A0A6J1L1N7 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500298 PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 2.1e-95
Identity = 171/252 (67.86%), Postives = 209/252 (82.94%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           ME KNWLVLI+AAFVFV G+LKRLNGWYY A+L   W +LPPGDM WPLLGST+S++K+ 
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNF 63

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
            +G+P NF+  F+ RYGK DM+KTH+LGRA+IIVC+ ELCRQVLGDE+ F PSLP NI +
Sbjct: 64  ISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           L+G+KSL+Q  KAEH+RLRRL  APISGHAALEMYI HIE TV+ GLEEW SM+ PIELL
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRF 240
           +EIKKLTFK+IWNIFMGS++ + SIGEME LF +VALGF+SLPINFPGF+ +K+ K  + 
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARK- 243

Query: 241 YRFLIILFSSVN 253
            + L IL S ++
Sbjct: 244 -KLLDILQSIID 253

BLAST of MS026607 vs. ExPASy TrEMBL
Match: A0A6J1E5Q1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431019 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 8.1e-95
Identity = 174/252 (69.05%), Postives = 205/252 (81.35%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           ME KNWLVL++AAFVFV GVLKRLNGWYY A+L   W +LPPGDM WPLLGST+ F+K  
Sbjct: 4   MEVKNWLVLVVAAFVFVLGVLKRLNGWYYAAKLGKAWPKLPPGDMGWPLLGSTILFLKSF 63

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
           S G+P+NF+   F RYGK DM+KTH+LGRA+IIVCT ELCRQVLGDE  F PS PV+I  
Sbjct: 64  SCGQPRNFISNIFIRYGKGDMYKTHILGRATIIVCTSELCRQVLGDEHNFKPSFPVSINR 123

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           LSG+KSLIQ  KAEHRRLRRL  APISGHAAL+MYI HIE TV+ GLEEW SM+ PIELL
Sbjct: 124 LSGRKSLIQVFKAEHRRLRRLTMAPISGHAALKMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRF 240
           +E+KKLTFK+IWNIFMGS++ +YSI EM  LF +VALGF+SLPINFPGF+F+KS K  + 
Sbjct: 184 SEMKKLTFKIIWNIFMGSSSFEYSIDEMVVLFSEVALGFISLPINFPGFNFHKSLKARK- 243

Query: 241 YRFLIILFSSVN 253
            + L IL S ++
Sbjct: 244 -KLLKILQSIIH 253

BLAST of MS026607 vs. ExPASy TrEMBL
Match: A0A1S3BJR1 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.4e-94
Identity = 172/252 (68.25%), Postives = 207/252 (82.14%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           ME K WLVLI+ +   +FGV+KRLNGWYY A+L  +W +LPPGDM WP+LGSTLSF+K+ 
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
           ++G+P+  +H    RYGK DM+KTH+LGRASIIVCT E+CRQVL DEEKF PSLP +I L
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           LSG+KSL+Q  KAEHRRLRRL  APISGH+ALEMYI HIE+TV+ GLEEW SM+KPIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRF 240
           TEIKKLTFK+IWNIFMGS++ D SIGE+EALF  +ALGF+SLPINFPGFSF+KS K  + 
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK- 240

Query: 241 YRFLIILFSSVN 253
              L +L S +N
Sbjct: 241 -ELLSLLQSIIN 250

BLAST of MS026607 vs. ExPASy TrEMBL
Match: A0A1S3BKD0 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.4e-94
Identity = 172/252 (68.25%), Postives = 207/252 (82.14%), Query Frame = 0

Query: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDV 60
           ME K WLVLI+ +   +FGV+KRLNGWYY A+L  +W +LPPGDM WP+LGSTLSF+K+ 
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120
           ++G+P+  +H    RYGK DM+KTH+LGRASIIVCT E+CRQVL DEEKF PSLP +I L
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELL 180
           LSG+KSL+Q  KAEHRRLRRL  APISGH+ALEMYI HIE+TV+ GLEEW SM+KPIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRF 240
           TEIKKLTFK+IWNIFMGS++ D SIGE+EALF  +ALGF+SLPINFPGFSF+KS K  + 
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK- 240

Query: 241 YRFLIILFSSVN 253
              L +L S +N
Sbjct: 241 -ELLSLLQSIIN 250

BLAST of MS026607 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 178.7 bits (452), Expect = 5.8e-45
Identity = 92/246 (37.40%), Postives = 150/246 (60.98%), Query Frame = 0

Query: 6   WLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDVSNGRP 65
           W  LI+     +  VLKR+N W Y ++L      LPPGD+ WP++G+  SF++      P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 66  QNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKLLSGKK 125
           ++F+ ++  RYG+  ++K H+ G   ++V TPE CR+VL D++ F    P +   L G+K
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 126 SLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELLTEIKK 185
           S +  S  EH+RLRRL +AP++G  AL +YI  IEETV   LE+W  M + IE L+ ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 189

Query: 186 LTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRFYRFLI 245
           LTFKVI  IF+ S+ +++ +  +E  + ++  G  ++ IN PGF+++++ K     + L+
Sbjct: 190 LTFKVIMYIFL-SSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKAR---KKLV 249

Query: 246 ILFSSV 252
             F S+
Sbjct: 250 AAFQSI 250

BLAST of MS026607 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 178.7 bits (452), Expect = 5.8e-45
Identity = 92/246 (37.40%), Postives = 150/246 (60.98%), Query Frame = 0

Query: 6   WLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDVSNGRP 65
           W  LI+     +  VLKR+N W Y ++L      LPPGD+ WP++G+  SF++      P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 66  QNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKLLSGKK 125
           ++F+ ++  RYG+  ++K H+ G   ++V TPE CR+VL D++ F    P +   L G+K
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 126 SLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELLTEIKK 185
           S +  S  EH+RLRRL +AP++G  AL +YI  IEETV   LE+W  M + IE L+ ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 189

Query: 186 LTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRFYRFLI 245
           LTFKVI  IF+ S+ +++ +  +E  + ++  G  ++ IN PGF+++++ K     + L+
Sbjct: 190 LTFKVIMYIFL-SSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKAR---KKLV 249

Query: 246 ILFSSV 252
             F S+
Sbjct: 250 AAFQSI 250

BLAST of MS026607 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 170.6 bits (431), Expect = 1.6e-42
Identity = 87/246 (35.37%), Postives = 143/246 (58.13%), Query Frame = 0

Query: 6   WLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMNWPLLGSTLSFVKDVSNGRP 65
           W  L++     +  +++++N W Y + L      LPPGD+ WP +G+ LSF++      P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 66  QNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKLLSGKK 125
            +F  T   RYG   ++K H+ G  SIIV T + CR+VL D++ F P  P +   L G+K
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 126 SLIQFSKAEHRRLRRLMTAPISGHAALEMYICHIEETVVGGLEEWESMQKPIELLTEIKK 185
           S +  S  EH+RLRRL  AP++GH AL  YI +IEE V+  L++W  M +  E LT ++K
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRK 190

Query: 186 LTFKVIWNIFMGSTTTDYSIGEMEALFYDVALGFMSLPINFPGFSFYKSFKVSRFYRFLI 245
           LTF++I  IF+ S+ ++  +  +E  +  +  G  ++ +N PGF+++++ K     + L+
Sbjct: 191 LTFRIIMYIFL-SSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKAR---KTLV 250

Query: 246 ILFSSV 252
             F S+
Sbjct: 251 AAFQSI 251

BLAST of MS026607 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 99.4 bits (246), Expect = 4.5e-21
Identity = 59/202 (29.21%), Postives = 102/202 (50.50%), Query Frame = 0

Query: 39  ELPPGDMNWPLLGSTLSFVKDVSNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPE 98
           + P G + WP++G T+ FV    + RP++F+      YG+V  FK+H+ G A+I+    E
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRV--FKSHIFGTATIVSTDAE 109

Query: 99  LCRQVL-GDEEKFVPSLPVNIKLLSGKKSLIQFSKAEHRRLRRLMTAPISGHAALEMYIC 158
           + R VL  D   FVP  P  ++ L GK S++  + + HRR   L+ + +         + 
Sbjct: 110 VNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVR 169

Query: 159 HIEETVVGGLEEWESMQKPIELLTEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFYDVAL 218
            + + +   ++ W S  +P+ L    K + FKV+    + S      + E++  F +   
Sbjct: 170 DMHKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALI-SVEKGEDLEELKREFENFIS 229

Query: 219 GFMSLPINFPGFSFYKSFKVSR 240
           G MSLPINFPG   ++S +  +
Sbjct: 230 GLMSLPINFPGTQLHRSLQAKK 247

BLAST of MS026607 vs. TAIR 10
Match: AT1G73340.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 89.7 bits (221), Expect = 3.6e-18
Identity = 59/210 (28.10%), Postives = 109/210 (51.90%), Query Frame = 0

Query: 32  RLRNIWAELPPGDMNWPLLGSTLSFVKDVSNGRPQNFVHTFFNRYGKVDMFKTHVLGRAS 91
           R R     LPPG   WPL+G T +++  V+   P +FV     +YG++  F   + G+ +
Sbjct: 35  RKRRAPHRLPPGSRGWPLIGDTFAWLNAVAGSHPSSFVEKQIKKYGRI--FSCSLFGKWA 94

Query: 92  IIVCTPELCRQVLGDEEK-FVPSLPVNIKLLSGKKSLIQFSKAEHRRLRRLMTAPISGHA 151
           ++   P+  R ++ +E K F  S P + + L GK  +I     + RRL  + ++ +  H 
Sbjct: 95  VVSADPDFNRFIMQNEGKLFQSSYPKSFRDLVGKDGVITVHGDQQRRLHSIASS-MMRHD 154

Query: 152 ALEMYICHIEETVVGGLEEWESMQK-PIELLTEI-KKLTFKVIWNIFMGSTTTDYSIGEM 211
            L+ +   +   V+  L+   + +   + LL +I +K+   ++ N  +G  +++  + EM
Sbjct: 155 QLKTHFLEVIPVVM--LQTLSNFKDGEVVLLQDICRKVAIHLMVNQLLG-VSSESEVDEM 214

Query: 212 EALFYDVALGFMSLPINFPGFSFYKSFKVS 239
             LF D   G +S+PI+ PGF++ K+ KVS
Sbjct: 215 SQLFSDFVDGCLSVPIDLPGFTYNKAMKVS 238

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145285.11.3e-13197.07beta-amyrin 11-oxidase-like [Momordica charantia][more]
XP_023007796.14.4e-9567.86beta-amyrin 11-oxidase-like isoform X1 [Cucurbita maxima][more]
XP_023552542.11.3e-9467.46beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo][more]
XP_022923277.11.7e-9469.05beta-amyrin 11-oxidase-like [Cucurbita moschata][more]
KAG6577627.11.7e-9469.05Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
B5BSX11.4e-5645.65Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1[more]
Q9C5Y28.2e-4437.40Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
O230512.2e-4135.37Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q5VRM72.9e-4136.64Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Q9AXH96.7e-3834.47Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CU166.3e-13297.07beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111014773 PE=3 ... [more]
A0A6J1L1N72.1e-9567.86beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1115002... [more]
A0A6J1E5Q18.1e-9569.05beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431019 PE=3 S... [more]
A0A1S3BJR11.4e-9468.25beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 P... [more]
A0A1S3BKD01.4e-9468.25beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490801 P... [more]
Match NameE-valueIdentityDescription
AT2G32440.15.8e-4537.40ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.25.8e-4537.40ent-kaurenoic acid hydroxylase 2 [more]
AT1G05160.11.6e-4235.37cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT3G13730.14.5e-2129.21cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
AT1G73340.13.6e-1828.10Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 33..246
e-value: 1.0E-32
score: 115.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 39..243
IPR001128Cytochrome P450PFAMPF00067p450coord: 41..200
e-value: 5.8E-12
score: 45.1
NoneNo IPR availablePANTHERPTHR24286:SF199CYTOCHROME P450 88D6coord: 18..236
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 18..236

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS026607.1MS026607.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen