MS026413 (gene) Bitter gourd (TR) v1

Overview
NameMS026413
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionberberine bridge enzyme-like 18
Locationscaffold402: 1126790 .. 1128217 (-)
RNA-Seq ExpressionMS026413
SyntenyMS026413
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGCTAAAACTCCAAAGCCTCAAGTAATCGTGACACCATTGCAAGTTTCCCACATTCAAGCATCCATCATTTGCGCAAGAAAGAACGGCTTTCAGATTCGAACAAGAAGTGGTGGCCATGACTACGAGGGTATTTCTTATGTTTCTGATGTTCCTTTTGTCCTTGTTGATTTCGTAAACCTTCGGTCGATCGACATTGATGTAGAAAGAAAAGTTGCATGGGTGCAATCCGGAGCAACCCTCGGCGAACTTTACTACAAAATTGCAGAGAAAAGCAAAACTTTAGGGTACCCAGGTGGAGTTTGCCCGACAGTTGGAGTTGGCGGGCACTTCAGCGGCGGTGGCTATGGTGTTATGCTACGAAAATTTGGCCTCGCTATTGATAATGTGATTGACGCTTACCTCGTTGATGTTAACGGTAAGCTCCACGATCGGAAATCGATGGGTGAGGATGTGTTTTGGGCCATTAGAGGAGGAGGATCAAGCTTTGGAATTATTCTTTCATGGAAAATAAAGCTCGTCCGAGTTCCACCGGTGGTGACTGTTTTCACAATTAAAAGAAACTTAGAAGAAAATGCCACCGATTTAGTCCATCGGTGGCAATACGTGGCCAATAAACAAGATGATAGACTTGTCATCAGAATCATATTAAGTGGCATGAAGTCAAGCAATCACCAAAATGGAAAAAGACTATCAATTGAAGCATCTTTCAGCTCCTTGTTCCTAGGAAAAGTTGAAGAGCTTCTTCCCATCATGCAAAGGACTTTCCCTGAACTTGGGATGACGAAAGAAGATTGTACAGAAATGAGCTGGATTGAATCGGTTCTCTATTTTGCCGACTTCGACCCTAAAAGACAGCCATTAGATGTCCTTATCGATAGGAGCTTTGCTTTTCGAGGCGTCTTCAAAGGCAAATCTGACTATGTAGATGAGCCAATTCCCAAAACTGGGCTCATCGGTTTATGGGAATTACTCAACGAAAAAGAAGTTCAATTTGCACGAATGCATCTTATCCCTTATGGAGGAAAAATGAATGAAATTTCTGAATTAGAAACTCCCTATCCACATAGAGCTGGAAATCTTTACAACATTCATTATGTGGTATCTTGGGGAGGAGACGAAGATATTGCACAAAAACATATAAGTTGGATCCGTAAGCTTTACAATTACATGACTCCTTTCGTTTCAAAAAATCCGAGAGGTGCATATGTCAATTACCGAGATCTAGATCTTGGAATAAACAACATAAATGAGTACACGAGCTATGAAAAAGCAAGCATATGGGGACTTAAATACTATAAGGATAATTTCGAGAGGTTGGTGAGCATAAAAACTAAGGTTGATCCTACTGATTTCTTTACGAATCAGCAGAGCATTCCTTCTCTTTTGTCATCGAAGAAGAGGAGAGAACATGAGACGTCTTGA

mRNA sequence

ATGGGTGCTAAAACTCCAAAGCCTCAAGTAATCGTGACACCATTGCAAGTTTCCCACATTCAAGCATCCATCATTTGCGCAAGAAAGAACGGCTTTCAGATTCGAACAAGAAGTGGTGGCCATGACTACGAGGGTATTTCTTATGTTTCTGATGTTCCTTTTGTCCTTGTTGATTTCGTAAACCTTCGGTCGATCGACATTGATGTAGAAAGAAAAGTTGCATGGGTGCAATCCGGAGCAACCCTCGGCGAACTTTACTACAAAATTGCAGAGAAAAGCAAAACTTTAGGGTACCCAGGTGGAGTTTGCCCGACAGTTGGAGTTGGCGGGCACTTCAGCGGCGGTGGCTATGGTGTTATGCTACGAAAATTTGGCCTCGCTATTGATAATGTGATTGACGCTTACCTCGTTGATGTTAACGGTAAGCTCCACGATCGGAAATCGATGGGTGAGGATGTGTTTTGGGCCATTAGAGGAGGAGGATCAAGCTTTGGAATTATTCTTTCATGGAAAATAAAGCTCGTCCGAGTTCCACCGGTGGTGACTGTTTTCACAATTAAAAGAAACTTAGAAGAAAATGCCACCGATTTAGTCCATCGGTGGCAATACGTGGCCAATAAACAAGATGATAGACTTGTCATCAGAATCATATTAAGTGGCATGAAGTCAAGCAATCACCAAAATGGAAAAAGACTATCAATTGAAGCATCTTTCAGCTCCTTGTTCCTAGGAAAAGTTGAAGAGCTTCTTCCCATCATGCAAAGGACTTTCCCTGAACTTGGGATGACGAAAGAAGATTGTACAGAAATGAGCTGGATTGAATCGGTTCTCTATTTTGCCGACTTCGACCCTAAAAGACAGCCATTAGATGTCCTTATCGATAGGAGCTTTGCTTTTCGAGGCGTCTTCAAAGGCAAATCTGACTATGTAGATGAGCCAATTCCCAAAACTGGGCTCATCGGTTTATGGGAATTACTCAACGAAAAAGAAGTTCAATTTGCACGAATGCATCTTATCCCTTATGGAGGAAAAATGAATGAAATTTCTGAATTAGAAACTCCCTATCCACATAGAGCTGGAAATCTTTACAACATTCATTATGTGGTATCTTGGGGAGGAGACGAAGATATTGCACAAAAACATATAAGTTGGATCCGTAAGCTTTACAATTACATGACTCCTTTCGTTTCAAAAAATCCGAGAGGTGCATATGTCAATTACCGAGATCTAGATCTTGGAATAAACAACATAAATGAGTACACGAGCTATGAAAAAGCAAGCATATGGGGACTTAAATACTATAAGGATAATTTCGAGAGGTTGGTGAGCATAAAAACTAAGGTTGATCCTACTGATTTCTTTACGAATCAGCAGAGCATTCCTTCTCTTTTGTCATCGAAGAAGAGGAGAGAACATGAGACGTCTTGA

Coding sequence (CDS)

ATGGGTGCTAAAACTCCAAAGCCTCAAGTAATCGTGACACCATTGCAAGTTTCCCACATTCAAGCATCCATCATTTGCGCAAGAAAGAACGGCTTTCAGATTCGAACAAGAAGTGGTGGCCATGACTACGAGGGTATTTCTTATGTTTCTGATGTTCCTTTTGTCCTTGTTGATTTCGTAAACCTTCGGTCGATCGACATTGATGTAGAAAGAAAAGTTGCATGGGTGCAATCCGGAGCAACCCTCGGCGAACTTTACTACAAAATTGCAGAGAAAAGCAAAACTTTAGGGTACCCAGGTGGAGTTTGCCCGACAGTTGGAGTTGGCGGGCACTTCAGCGGCGGTGGCTATGGTGTTATGCTACGAAAATTTGGCCTCGCTATTGATAATGTGATTGACGCTTACCTCGTTGATGTTAACGGTAAGCTCCACGATCGGAAATCGATGGGTGAGGATGTGTTTTGGGCCATTAGAGGAGGAGGATCAAGCTTTGGAATTATTCTTTCATGGAAAATAAAGCTCGTCCGAGTTCCACCGGTGGTGACTGTTTTCACAATTAAAAGAAACTTAGAAGAAAATGCCACCGATTTAGTCCATCGGTGGCAATACGTGGCCAATAAACAAGATGATAGACTTGTCATCAGAATCATATTAAGTGGCATGAAGTCAAGCAATCACCAAAATGGAAAAAGACTATCAATTGAAGCATCTTTCAGCTCCTTGTTCCTAGGAAAAGTTGAAGAGCTTCTTCCCATCATGCAAAGGACTTTCCCTGAACTTGGGATGACGAAAGAAGATTGTACAGAAATGAGCTGGATTGAATCGGTTCTCTATTTTGCCGACTTCGACCCTAAAAGACAGCCATTAGATGTCCTTATCGATAGGAGCTTTGCTTTTCGAGGCGTCTTCAAAGGCAAATCTGACTATGTAGATGAGCCAATTCCCAAAACTGGGCTCATCGGTTTATGGGAATTACTCAACGAAAAAGAAGTTCAATTTGCACGAATGCATCTTATCCCTTATGGAGGAAAAATGAATGAAATTTCTGAATTAGAAACTCCCTATCCACATAGAGCTGGAAATCTTTACAACATTCATTATGTGGTATCTTGGGGAGGAGACGAAGATATTGCACAAAAACATATAAGTTGGATCCGTAAGCTTTACAATTACATGACTCCTTTCGTTTCAAAAAATCCGAGAGGTGCATATGTCAATTACCGAGATCTAGATCTTGGAATAAACAACATAAATGAGTACACGAGCTATGAAAAAGCAAGCATATGGGGACTTAAATACTATAAGGATAATTTCGAGAGGTTGGTGAGCATAAAAACTAAGGTTGATCCTACTGATTTCTTTACGAATCAGCAGAGCATTCCTTCTCTTTTGTCATCGAAGAAGAGGAGAGAACATGAGACGTCTTGA

Protein sequence

MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIRGGGSSFGIILSWKIKLVRVPPVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHETS
Homology
BLAST of MS026413 vs. NCBI nr
Match: XP_022152426.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 961.4 bits (2484), Expect = 2.8e-276
Identity = 468/475 (98.53%), Postives = 472/475 (99.37%), Query Frame = 0

Query: 1   MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 60
           MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV
Sbjct: 69  MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 128

Query: 61  NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 120
           NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM
Sbjct: 129 NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 188

Query: 121 LRKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIRGGGSSFGIILSWKIKLVRVPPV 180
           LRKFGLAIDNVIDAYLVDVNG+LHDRKSMGEDVFWAIRGGG+SFGIILSWKIKLV VP V
Sbjct: 189 LRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKIKLVSVPSV 248

Query: 181 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 240
           VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS
Sbjct: 249 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 308

Query: 241 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFR 300
           LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADF+PKRQPLDVLIDRSFAFR
Sbjct: 309 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVLIDRSFAFR 368

Query: 301 GVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAG 360
           GVFKGKSDYVDEPIPKTGLIGLWELLN+KEVQFARMHLIPYGGKMNEISE ETPYPHRAG
Sbjct: 369 GVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESETPYPHRAG 428

Query: 361 NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY 420
           NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY
Sbjct: 429 NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY 488

Query: 421 TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHETS 476
           TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHETS
Sbjct: 489 TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHETS 543

BLAST of MS026413 vs. NCBI nr
Match: XP_022152520.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 773.1 bits (1995), Expect = 1.4e-219
Identity = 363/474 (76.58%), Postives = 417/474 (87.97%), Query Frame = 0

Query: 1   MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 60
           +GAKTPKPQ+IVTPL+ SHIQAS+ICA+ NGFQIRTRSGGHDYEG+SYVSDVPFVLVD  
Sbjct: 68  LGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVPFVLVDLT 127

Query: 61  NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 120
           N+RSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHFSGGGYG+M
Sbjct: 128 NIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYGLM 187

Query: 121 LRKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIRGGGSSFGIILSWKIKLVRVPPV 180
           LRK+GLA DNVIDAY +DVNG+LHDRKSMGEDVFWAIRGGG+SFGIIL+WK+KLV VPPV
Sbjct: 188 LRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKVKLVLVPPV 247

Query: 181 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 240
           VTVF+I RNL++NAT+LVHRWQYVANKQDDRL+IR +L    S NHQNGKR +++A+F+S
Sbjct: 248 VTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKR-TVKANFNS 307

Query: 241 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFR 300
           LFLGKV ELLPIMQ TFPELG+ KEDC EMSWIESVLYF+DF P+ QPL+VLIDR+ A +
Sbjct: 308 LFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDF-PREQPLNVLIDRNLALK 367

Query: 301 GVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAG 360
           G+FKGKSDY+++PIPK GL GLWELL   EV+F RMHL+PYGGKMNEISE + P+PHRA 
Sbjct: 368 GIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPFPHRAE 427

Query: 361 NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY 420
           NLY IHYVVSW    D  Q+HISWIRKLY YMTPFVSKNPR AY NYRDLD+G NN +  
Sbjct: 428 NLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTNNKDGN 487

Query: 421 TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHET 475
           T+YEKASIWG KYYKDNF+RLVSIKTKVDP++FF N+QSIPSL    K   HET
Sbjct: 488 TNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHET 539

BLAST of MS026413 vs. NCBI nr
Match: XP_022152423.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 752.7 bits (1942), Expect = 2.0e-213
Identity = 363/473 (76.74%), Postives = 421/473 (89.01%), Query Frame = 0

Query: 3   AKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSD-VPFVLVDFVN 62
           AKTPKPQVIVTPLQVSHIQASIICA+ NGFQIRTRSGGHDYEG+SYVSD VPFV++D  N
Sbjct: 71  AKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDNVPFVVIDLTN 130

Query: 63  LRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVML 122
           LRSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+C TVGVGGHFSGGGYGVML
Sbjct: 131 LRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFSGGGYGVML 190

Query: 123 RKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPV 182
           RKFGLA DNVIDAY +DVNG L DRKSMGEDVFWAIR GGG+SFGIIL+WKIKL+ VPPV
Sbjct: 191 RKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKIKLLPVPPV 250

Query: 183 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGM-KSSNHQNGKRLSIEASFS 242
           VTVFTI RNLE+NAT+L+H+WQYVA+KQDDRL+IR+IL+GM  SSN+QN K+ ++EASF+
Sbjct: 251 VTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNXKK-TLEASFN 310

Query: 243 SLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAF 302
           SLFLG+V+ELLPIMQ+TFPELG+TKEDC EMSWIE+VLYFA F  + QPL+VL+DRS A+
Sbjct: 311 SLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF--QGQPLNVLLDRSLAW 370

Query: 303 RGVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRA 362
           +G FKGKSDYV+EPIPK G+ G+WEL NE+EVQFA+M LIPYGGKM+EISE E P+PHR 
Sbjct: 371 KGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEXEIPFPHRV 430

Query: 363 GNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINE 422
           GNLY IHY V  G  ED AQ+ ISWIRKLY+YMTPFVSKNPR AY+NYRDLD+G NN + 
Sbjct: 431 GNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDIGTNNKDG 490

Query: 423 YTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 473
            TSY+KASIWG+KY+K NF+RLVS+KTKVDP++FF N+QSIPSL    K+R H
Sbjct: 491 NTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRGH 540

BLAST of MS026413 vs. NCBI nr
Match: XP_022152425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 751.1 bits (1938), Expect = 5.7e-213
Identity = 352/463 (76.03%), Postives = 413/463 (89.20%), Query Frame = 0

Query: 1   MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 60
           +GAKTPKPQ+IVTPL+ SH+QASIICA+ NG QIRTRSGGHDYEG+SYVS+VPFV+VD  
Sbjct: 69  LGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNVPFVVVDLT 128

Query: 61  NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 120
           NLRSID+DVE+KVAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHFSGGGYGVM
Sbjct: 129 NLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYGVM 188

Query: 121 LRKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPP 180
           LRKFGLA DNVIDAY +DVNGKLHDRKSMGEDVFWAIR GGG+SFGI+L+WKIKL++VP 
Sbjct: 189 LRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWKIKLLQVPX 248

Query: 181 VVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFS 240
           +VTVFTI RNLE+NAT+L++RWQYVANKQDDRL+I+IIL G  S+NH+NGKR +IEA F+
Sbjct: 249 LVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKR-TIEAFFN 308

Query: 241 SLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAF 300
           SLFLGKVE+LLPIMQ+TFPELG+ KEDC EMSWIESVLYFA+F P+ QPL+VL+DR   F
Sbjct: 309 SLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEF-PRGQPLNVLLDRRSTF 368

Query: 301 RGVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRA 360
           +G FKGKSDYV+EP+PK GL G+WEL NEKE +  +M LIPYGGKMNEISE E P+PHRA
Sbjct: 369 KGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISESEIPFPHRA 428

Query: 361 GNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINE 420
           GNLY IHY +  GGDE+  Q++  WIRK Y+YM PFVSKNPR AY+NYRDLD+G NN N 
Sbjct: 429 GNLYQIHYSIYCGGDEE--QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLDIGTNNENG 488

Query: 421 YTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPS 463
            TSY+KASIWG+KY+KDNF+RLVS+K KVDP++FF N+QSIPS
Sbjct: 489 NTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPS 527

BLAST of MS026413 vs. NCBI nr
Match: XP_022152442.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 736.9 bits (1901), Expect = 1.1e-208
Identity = 344/475 (72.42%), Postives = 407/475 (85.68%), Query Frame = 0

Query: 1   MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 60
           +G KT KPQVIVTPLQ SHIQASIICA+KNGFQIR RSGGHDYEG+SYVSDVPFV+VD +
Sbjct: 69  LGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDVPFVVVDMI 128

Query: 61  NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 120
           NLRSID+DVE++VAWV+SGAT+GELYY+IAEKSKTL +P G+  TVGVGGHFSGGGYG +
Sbjct: 129 NLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGHFSGGGYGSL 188

Query: 121 LRKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIRGGGSSFGIILSWKIKLVRVPPV 180
           +RKFGLA DNVIDAY +DVNGKLHDR+SMGED+FWAIRGGG+SFGIILSWK+KLV VPP+
Sbjct: 189 IRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKVKLVSVPPM 248

Query: 181 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 240
           VT+F I RNLE+NATDLVH+WQYVANKQDDRL+IRIIL+    +NHQNGKR+ ++A+F  
Sbjct: 249 VTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRIVLQAAFKG 308

Query: 241 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFR 300
            FLGKVEELLPIMQ TFPELG+ KED  EMSWIES++YFA   PK Q    L+DRS A +
Sbjct: 309 FFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKL-PKGQ-ASALLDRSLALK 368

Query: 301 GVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAG 360
           G +KGK DYV+EPIPK GL G+WELLNEK++ FA++ LIPYGG+M+EISE E P+PHRAG
Sbjct: 369 GTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIPFPHRAG 428

Query: 361 NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY 420
           N+Y IHY V++ GD +  Q+HI WIRK YNY+TPFVSKNPR  Y+NYRDLD+G+NN N  
Sbjct: 429 NIYKIHYTVAYSGDAE--QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNNKNGC 488

Query: 421 TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHETS 476
           TSYEKAS+WGLKY+K NF+RLV +K KVDPT+FF N+QSIPS L+  K+  HE S
Sbjct: 489 TSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEMS 539

BLAST of MS026413 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.3e-147
Identity = 244/457 (53.39%), Postives = 339/457 (74.18%), Query Frame = 0

Query: 6   PKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLRSI 65
           PKP +I+TP+Q S +Q+++ CAR+ G  IRTRSGGHDYEG+SYV+  PFV++D  NLRSI
Sbjct: 82  PKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSI 141

Query: 66  DIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKFG 125
            +DV+ +  WVQ+GAT+GELYY+I +K++TL +P GVCPTVGVGGHFSGGGYG +LRK G
Sbjct: 142 TVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHG 201

Query: 126 LAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPVVTVF 185
           LA D+VIDA +VD  G++ +R+ MGED FWAIR GGGSSF ++LSWKI L+ VP  VTVF
Sbjct: 202 LAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVF 261

Query: 186 TIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLFLG 245
            + +  E++A  ++HRWQ+VA+K  D L IR++L   K+          + ASF  L+LG
Sbjct: 262 NVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKN---------MVRASFPGLYLG 321

Query: 246 KVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFRGVFK 305
            V+ LL ++ + FPELG+ ++DCTEMSWIESV++FA+     +P++VL  R+ A    FK
Sbjct: 322 SVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELG--EEPINVLTKRTRASL-AFK 381

Query: 306 GKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAGNLYN 365
            KSD+V EP+PKT +  LW  L E E + A++   P+GGKM+EI++ ETP+PHR GN+Y 
Sbjct: 382 AKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYE 441

Query: 366 IHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEYTSYE 425
           I Y+  W G  D+ +K++ W+ ++Y+ M+ FV+K+PRGAY+N RDLDLG+    + + YE
Sbjct: 442 IQYLNYWRG--DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYE 501

Query: 426 KASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIP 462
           +   WG+KY+K+NFERLV +KT VDP+DFF ++QSIP
Sbjct: 502 EGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MS026413 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.9e-143
Identity = 239/465 (51.40%), Postives = 331/465 (71.18%), Query Frame = 0

Query: 3   AKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVS-DVPFVLVDFVN 62
           ++TPKP  I+TP++ S +Q  I CA+ +G  +RTRS GH YEG+SY++ + PF ++D  N
Sbjct: 73  SETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRN 132

Query: 63  LRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVML 122
           LRSI +DV+ +  WVQ+GAT GELYY+I + +K+L +P G+ PTVGVGG FSGGGYG +L
Sbjct: 133 LRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLL 192

Query: 123 RKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPV 182
           RK+GLA DN+IDA +VD +G++ DR++MGED FWAIR GGGSSFG+ILSWK+KLV VP  
Sbjct: 193 RKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPST 252

Query: 183 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 242
           +TVF +++  ++ A  ++ +WQY A+K  D L IR  L        +   + ++ A F+ 
Sbjct: 253 ITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL--------ERSNKNAVHALFTG 312

Query: 243 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFR 302
           L++G V  LL +M+  FPELG+ KE C EMSWIESVL+FADF PK + L VL +R     
Sbjct: 313 LYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADF-PKGESLGVLTNRERTSL 372

Query: 303 GVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAG 362
             FKGK D+V EPIP+  +  +W  L   E +  ++ L P+GGKM+E++E ETP+PHR G
Sbjct: 373 S-FKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGG 432

Query: 363 NLYNIHYVVSWGGDED----IAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINN 422
           NLY I YV  W  +ED       K++ W+  +Y +MTP+VSK+PRGAYVN++D+DLG+  
Sbjct: 433 NLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYL 492

Query: 423 INEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIP 462
             + T YE+   WG+KY+K+NFERLV +KT+VDPTDFF ++QSIP
Sbjct: 493 GKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MS026413 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 3.8e-143
Identity = 239/464 (51.51%), Postives = 333/464 (71.77%), Query Frame = 0

Query: 5   TPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLRS 64
           TPKP VI+TPL VSHIQ +I+C++K G QIRTRSGGHD EG+SY+S VPFV+VD  N+ S
Sbjct: 78  TPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHS 137

Query: 65  IDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKF 124
           + IDV  + AWV++GATLGE+YY I E ++ L +P G CPTVG GGHFSGGGYG ++R +
Sbjct: 138 VKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNY 197

Query: 125 GLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPVVTV 184
           GLA DN+IDA+LV+V+GK+ DRKSMGED+FWAIR GGG +FGII +WKI+LV VP + T+
Sbjct: 198 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTI 257

Query: 185 FTIKRNLE-ENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLF 244
           F++K+N+E      LV++WQ +A   +  L++         +++Q   + +I + FSS+F
Sbjct: 258 FSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIF 317

Query: 245 LGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFA---DFDPKRQPLDVLIDRSFAF 304
            G V+ L+ +M ++FPELG+ K DC ++SWI+++++++   +++      ++L+DRS   
Sbjct: 318 HGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGR 377

Query: 305 RGVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRA 364
           +  F  K DYV +PIP+T ++ + E L E++V        PYGG M+EISE   P+PHRA
Sbjct: 378 KAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRA 437

Query: 365 GNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINE 424
           G +Y I Y+ SW   ED  +KHI+WIR +YN+ TP+VS+NPR AY+NYRDLDLG  N   
Sbjct: 438 GIMYEIWYIASWEKQED-NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES 497

Query: 425 YTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSL 464
             +Y +A IWG KY+  NF RLV +KTKVDP +FF N+QSIP L
Sbjct: 498 PNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of MS026413 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 3.8e-143
Identity = 245/464 (52.80%), Postives = 329/464 (70.91%), Query Frame = 0

Query: 5   TPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLRS 64
           TPKP VIVTP   SHIQA+I+C++K G QIRTRSGGHD EG+SY+S VPFV+VD  N+ S
Sbjct: 78  TPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHS 137

Query: 65  IDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKF 124
           I IDV  + AWV++GATLGE+YY I EK++ L +PGG CPTVGVGGHFSGGGYG ++R +
Sbjct: 138 IKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNY 197

Query: 125 GLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPVVTV 184
           GLA DN+IDA+LV+V+GK+ DRKSMGED+FWAIR GGG +FGII +WKIKLV VP   T+
Sbjct: 198 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTI 257

Query: 185 FTIKRNLE-ENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLF 244
           F++K+N+E      L ++WQ +A K D  LV+         +++    + ++   FSS+F
Sbjct: 258 FSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIF 317

Query: 245 LGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFA---DFDPKRQPLDVLIDRSFAF 304
            G V+ L+ +M ++FPELG+ K DC E SWI++ ++++   +F+      ++L+DRS   
Sbjct: 318 HGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGK 377

Query: 305 RGVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRA 364
           +  F  K DYV +PIP+T ++ + E L E++V      L PYGG M EISE   P+PHRA
Sbjct: 378 KTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRA 437

Query: 365 GNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINE 424
           G +Y + Y  SW   ED  +KHI+W+R +YN+ TP+VS+NPR AY+NYRDLDLG  N   
Sbjct: 438 GIMYELWYTASWEKQED-NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHAS 497

Query: 425 YTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSL 464
             +Y +A IWG KY+  NF RLV +KTKVDP +FF N+QSIP L
Sbjct: 498 PNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of MS026413 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 5.0e-143
Identity = 240/464 (51.72%), Postives = 331/464 (71.34%), Query Frame = 0

Query: 5   TPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLRS 64
           TPKP VI TPL VSHIQ +I+C++K G QIRTRSGGHD EG+SY+S VPFV+VD  N+ S
Sbjct: 78  TPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHS 137

Query: 65  IDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKF 124
           + IDV  + AWV+SGATLGE+YY I E ++ L +P G CPTVG GGHFSGGGYG ++R +
Sbjct: 138 VKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNY 197

Query: 125 GLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPVVTV 184
           GLA DN+IDA+LV+V+GK+ DRKSMGED+FWAIR GGG +FGII +WKI+LV VP + T+
Sbjct: 198 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTI 257

Query: 185 FTIKRNLE-ENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLF 244
           F++K+N+E      LV++WQ +A   +  L++         +++Q   + +I + FSS+F
Sbjct: 258 FSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIF 317

Query: 245 LGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFA---DFDPKRQPLDVLIDRSFAF 304
            G V+ L+ +M ++FPELG+ K DC ++SWI+++++++   +++      ++L+DRS   
Sbjct: 318 HGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGR 377

Query: 305 RGVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRA 364
           +  F  K DYV +PIP+T ++ + E L E++V        PYGG M+EISE   P+PHRA
Sbjct: 378 KAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRA 437

Query: 365 GNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINE 424
           G  Y I Y+ SW   ED  +KHI+WIR +YN+ TP+VS+NPR AY+NYRDLDLG  N   
Sbjct: 438 GITYEIWYIASWEKQED-NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES 497

Query: 425 YTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSL 464
             +Y +A IWG KY+  NF RLV +KTKVDP +FF N+QSIP L
Sbjct: 498 PNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of MS026413 vs. ExPASy TrEMBL
Match: A0A6J1DHP3 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020162 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.3e-276
Identity = 468/475 (98.53%), Postives = 472/475 (99.37%), Query Frame = 0

Query: 1   MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 60
           MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV
Sbjct: 69  MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 128

Query: 61  NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 120
           NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM
Sbjct: 129 NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 188

Query: 121 LRKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIRGGGSSFGIILSWKIKLVRVPPV 180
           LRKFGLAIDNVIDAYLVDVNG+LHDRKSMGEDVFWAIRGGG+SFGIILSWKIKLV VP V
Sbjct: 189 LRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKIKLVSVPSV 248

Query: 181 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 240
           VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS
Sbjct: 249 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 308

Query: 241 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFR 300
           LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADF+PKRQPLDVLIDRSFAFR
Sbjct: 309 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVLIDRSFAFR 368

Query: 301 GVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAG 360
           GVFKGKSDYVDEPIPKTGLIGLWELLN+KEVQFARMHLIPYGGKMNEISE ETPYPHRAG
Sbjct: 369 GVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESETPYPHRAG 428

Query: 361 NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY 420
           NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY
Sbjct: 429 NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY 488

Query: 421 TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHETS 476
           TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHETS
Sbjct: 489 TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHETS 543

BLAST of MS026413 vs. ExPASy TrEMBL
Match: A0A6J1DE62 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.8e-220
Identity = 363/474 (76.58%), Postives = 417/474 (87.97%), Query Frame = 0

Query: 1   MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 60
           +GAKTPKPQ+IVTPL+ SHIQAS+ICA+ NGFQIRTRSGGHDYEG+SYVSDVPFVLVD  
Sbjct: 68  LGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVPFVLVDLT 127

Query: 61  NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 120
           N+RSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHFSGGGYG+M
Sbjct: 128 NIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYGLM 187

Query: 121 LRKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIRGGGSSFGIILSWKIKLVRVPPV 180
           LRK+GLA DNVIDAY +DVNG+LHDRKSMGEDVFWAIRGGG+SFGIIL+WK+KLV VPPV
Sbjct: 188 LRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKVKLVLVPPV 247

Query: 181 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 240
           VTVF+I RNL++NAT+LVHRWQYVANKQDDRL+IR +L    S NHQNGKR +++A+F+S
Sbjct: 248 VTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKR-TVKANFNS 307

Query: 241 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFR 300
           LFLGKV ELLPIMQ TFPELG+ KEDC EMSWIESVLYF+DF P+ QPL+VLIDR+ A +
Sbjct: 308 LFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDF-PREQPLNVLIDRNLALK 367

Query: 301 GVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAG 360
           G+FKGKSDY+++PIPK GL GLWELL   EV+F RMHL+PYGGKMNEISE + P+PHRA 
Sbjct: 368 GIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPFPHRAE 427

Query: 361 NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY 420
           NLY IHYVVSW    D  Q+HISWIRKLY YMTPFVSKNPR AY NYRDLD+G NN +  
Sbjct: 428 NLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTNNKDGN 487

Query: 421 TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHET 475
           T+YEKASIWG KYYKDNF+RLVSIKTKVDP++FF N+QSIPSL    K   HET
Sbjct: 488 TNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHET 539

BLAST of MS026413 vs. ExPASy TrEMBL
Match: A0A6J1DET9 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 PE=3 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 9.5e-214
Identity = 363/473 (76.74%), Postives = 421/473 (89.01%), Query Frame = 0

Query: 3   AKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSD-VPFVLVDFVN 62
           AKTPKPQVIVTPLQVSHIQASIICA+ NGFQIRTRSGGHDYEG+SYVSD VPFV++D  N
Sbjct: 71  AKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDNVPFVVIDLTN 130

Query: 63  LRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVML 122
           LRSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+C TVGVGGHFSGGGYGVML
Sbjct: 131 LRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFSGGGYGVML 190

Query: 123 RKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPV 182
           RKFGLA DNVIDAY +DVNG L DRKSMGEDVFWAIR GGG+SFGIIL+WKIKL+ VPPV
Sbjct: 191 RKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKIKLLPVPPV 250

Query: 183 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGM-KSSNHQNGKRLSIEASFS 242
           VTVFTI RNLE+NAT+L+H+WQYVA+KQDDRL+IR+IL+GM  SSN+QN K+ ++EASF+
Sbjct: 251 VTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNXKK-TLEASFN 310

Query: 243 SLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAF 302
           SLFLG+V+ELLPIMQ+TFPELG+TKEDC EMSWIE+VLYFA F  + QPL+VL+DRS A+
Sbjct: 311 SLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF--QGQPLNVLLDRSLAW 370

Query: 303 RGVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRA 362
           +G FKGKSDYV+EPIPK G+ G+WEL NE+EVQFA+M LIPYGGKM+EISE E P+PHR 
Sbjct: 371 KGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEXEIPFPHRV 430

Query: 363 GNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINE 422
           GNLY IHY V  G  ED AQ+ ISWIRKLY+YMTPFVSKNPR AY+NYRDLD+G NN + 
Sbjct: 431 GNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDIGTNNKDG 490

Query: 423 YTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 473
            TSY+KASIWG+KY+K NF+RLVS+KTKVDP++FF N+QSIPSL    K+R H
Sbjct: 491 NTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRGH 540

BLAST of MS026413 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 2.8e-213
Identity = 352/463 (76.03%), Postives = 413/463 (89.20%), Query Frame = 0

Query: 1   MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 60
           +GAKTPKPQ+IVTPL+ SH+QASIICA+ NG QIRTRSGGHDYEG+SYVS+VPFV+VD  
Sbjct: 69  LGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNVPFVVVDLT 128

Query: 61  NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 120
           NLRSID+DVE+KVAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHFSGGGYGVM
Sbjct: 129 NLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYGVM 188

Query: 121 LRKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPP 180
           LRKFGLA DNVIDAY +DVNGKLHDRKSMGEDVFWAIR GGG+SFGI+L+WKIKL++VP 
Sbjct: 189 LRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWKIKLLQVPX 248

Query: 181 VVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFS 240
           +VTVFTI RNLE+NAT+L++RWQYVANKQDDRL+I+IIL G  S+NH+NGKR +IEA F+
Sbjct: 249 LVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKR-TIEAFFN 308

Query: 241 SLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAF 300
           SLFLGKVE+LLPIMQ+TFPELG+ KEDC EMSWIESVLYFA+F P+ QPL+VL+DR   F
Sbjct: 309 SLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEF-PRGQPLNVLLDRRSTF 368

Query: 301 RGVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRA 360
           +G FKGKSDYV+EP+PK GL G+WEL NEKE +  +M LIPYGGKMNEISE E P+PHRA
Sbjct: 369 KGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISESEIPFPHRA 428

Query: 361 GNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINE 420
           GNLY IHY +  GGDE+  Q++  WIRK Y+YM PFVSKNPR AY+NYRDLD+G NN N 
Sbjct: 429 GNLYQIHYSIYCGGDEE--QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLDIGTNNENG 488

Query: 421 YTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPS 463
            TSY+KASIWG+KY+KDNF+RLVS+K KVDP++FF N+QSIPS
Sbjct: 489 NTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPS 527

BLAST of MS026413 vs. ExPASy TrEMBL
Match: A0A6J1DG99 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020173 PE=3 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 5.4e-209
Identity = 344/475 (72.42%), Postives = 407/475 (85.68%), Query Frame = 0

Query: 1   MGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFV 60
           +G KT KPQVIVTPLQ SHIQASIICA+KNGFQIR RSGGHDYEG+SYVSDVPFV+VD +
Sbjct: 69  LGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDVPFVVVDMI 128

Query: 61  NLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVM 120
           NLRSID+DVE++VAWV+SGAT+GELYY+IAEKSKTL +P G+  TVGVGGHFSGGGYG +
Sbjct: 129 NLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGHFSGGGYGSL 188

Query: 121 LRKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIRGGGSSFGIILSWKIKLVRVPPV 180
           +RKFGLA DNVIDAY +DVNGKLHDR+SMGED+FWAIRGGG+SFGIILSWK+KLV VPP+
Sbjct: 189 IRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKVKLVSVPPM 248

Query: 181 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 240
           VT+F I RNLE+NATDLVH+WQYVANKQDDRL+IRIIL+    +NHQNGKR+ ++A+F  
Sbjct: 249 VTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRIVLQAAFKG 308

Query: 241 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFR 300
            FLGKVEELLPIMQ TFPELG+ KED  EMSWIES++YFA   PK Q    L+DRS A +
Sbjct: 309 FFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKL-PKGQ-ASALLDRSLALK 368

Query: 301 GVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAG 360
           G +KGK DYV+EPIPK GL G+WELLNEK++ FA++ LIPYGG+M+EISE E P+PHRAG
Sbjct: 369 GTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIPFPHRAG 428

Query: 361 NLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEY 420
           N+Y IHY V++ GD +  Q+HI WIRK YNY+TPFVSKNPR  Y+NYRDLD+G+NN N  
Sbjct: 429 NIYKIHYTVAYSGDAE--QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNNKNGC 488

Query: 421 TSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREHETS 476
           TSYEKAS+WGLKY+K NF+RLV +K KVDPT+FF N+QSIPS L+  K+  HE S
Sbjct: 489 TSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEMS 539

BLAST of MS026413 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 523.1 bits (1346), Expect = 2.4e-148
Identity = 244/457 (53.39%), Postives = 339/457 (74.18%), Query Frame = 0

Query: 6   PKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLRSI 65
           PKP +I+TP+Q S +Q+++ CAR+ G  IRTRSGGHDYEG+SYV+  PFV++D  NLRSI
Sbjct: 82  PKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSI 141

Query: 66  DIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKFG 125
            +DV+ +  WVQ+GAT+GELYY+I +K++TL +P GVCPTVGVGGHFSGGGYG +LRK G
Sbjct: 142 TVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHG 201

Query: 126 LAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPVVTVF 185
           LA D+VIDA +VD  G++ +R+ MGED FWAIR GGGSSF ++LSWKI L+ VP  VTVF
Sbjct: 202 LAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVF 261

Query: 186 TIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLFLG 245
            + +  E++A  ++HRWQ+VA+K  D L IR++L   K+          + ASF  L+LG
Sbjct: 262 NVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKN---------MVRASFPGLYLG 321

Query: 246 KVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFRGVFK 305
            V+ LL ++ + FPELG+ ++DCTEMSWIESV++FA+     +P++VL  R+ A    FK
Sbjct: 322 SVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELG--EEPINVLTKRTRASL-AFK 381

Query: 306 GKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAGNLYN 365
            KSD+V EP+PKT +  LW  L E E + A++   P+GGKM+EI++ ETP+PHR GN+Y 
Sbjct: 382 AKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYE 441

Query: 366 IHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEYTSYE 425
           I Y+  W G  D+ +K++ W+ ++Y+ M+ FV+K+PRGAY+N RDLDLG+    + + YE
Sbjct: 442 IQYLNYWRG--DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYE 501

Query: 426 KASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIP 462
           +   WG+KY+K+NFERLV +KT VDP+DFF ++QSIP
Sbjct: 502 EGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MS026413 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 510.0 bits (1312), Expect = 2.1e-144
Identity = 239/465 (51.40%), Postives = 331/465 (71.18%), Query Frame = 0

Query: 3   AKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVS-DVPFVLVDFVN 62
           ++TPKP  I+TP++ S +Q  I CA+ +G  +RTRS GH YEG+SY++ + PF ++D  N
Sbjct: 73  SETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRN 132

Query: 63  LRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVML 122
           LRSI +DV+ +  WVQ+GAT GELYY+I + +K+L +P G+ PTVGVGG FSGGGYG +L
Sbjct: 133 LRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLL 192

Query: 123 RKFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPV 182
           RK+GLA DN+IDA +VD +G++ DR++MGED FWAIR GGGSSFG+ILSWK+KLV VP  
Sbjct: 193 RKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPST 252

Query: 183 VTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSS 242
           +TVF +++  ++ A  ++ +WQY A+K  D L IR  L        +   + ++ A F+ 
Sbjct: 253 ITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL--------ERSNKNAVHALFTG 312

Query: 243 LFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFR 302
           L++G V  LL +M+  FPELG+ KE C EMSWIESVL+FADF PK + L VL +R     
Sbjct: 313 LYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADF-PKGESLGVLTNRERTSL 372

Query: 303 GVFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAG 362
             FKGK D+V EPIP+  +  +W  L   E +  ++ L P+GGKM+E++E ETP+PHR G
Sbjct: 373 S-FKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGG 432

Query: 363 NLYNIHYVVSWGGDED----IAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINN 422
           NLY I YV  W  +ED       K++ W+  +Y +MTP+VSK+PRGAYVN++D+DLG+  
Sbjct: 433 NLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYL 492

Query: 423 INEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIP 462
             + T YE+   WG+KY+K+NFERLV +KT+VDPTDFF ++QSIP
Sbjct: 493 GKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MS026413 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 506.1 bits (1302), Expect = 3.0e-143
Identity = 244/463 (52.70%), Postives = 336/463 (72.57%), Query Frame = 0

Query: 7   KPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVS-DVPFVLVDFVNLRSI 66
           KP +IVTP   + IQ S++C+RK G ++RT+SGGHDYEG+SY+S   PF+++D VN+RSI
Sbjct: 83  KPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLSLHSPFIILDLVNVRSI 142

Query: 67  DIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKFG 126
           +I++  + AWV +GAT+GELYYKIA+ SK  G+P G CP+VGVGGHFSGGG+G M+RK G
Sbjct: 143 EINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVGGHFSGGGFGAMMRKHG 202

Query: 127 LAIDNVIDAYLVDVNGKLHD-RKSMGEDVFWAIRGGG-SSFGIILSWKIKLVRVPPVVTV 186
           LA DNV+DA  VD NG++++ R+ MGED+FWAIRGGG +SFG++LSWK+KLVRVP  VT 
Sbjct: 203 LAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVVLSWKVKLVRVPEKVTC 262

Query: 187 FTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLFL 246
           F     L +N T +VHRWQ +A + DD L IR+I+S             S++ +F + +L
Sbjct: 263 FRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGG---------SVQTTFQANYL 322

Query: 247 GKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFRGV- 306
           G +++L+P+M + FPELG+T +DC+EM+WI+S++YF     K QPL+ L+DR   +  + 
Sbjct: 323 GGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYFN--WKKGQPLETLLDRGQRYNDLY 382

Query: 307 FKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAGNL 366
           FK KSD+V  PIP+ GL G+W   +  EV+   M + P GGKM EI E ETP+PHR GNL
Sbjct: 383 FKAKSDFVKNPIPEIGLEGIWTRFH--EVESPIMIMEPLGGKMYEIGETETPFPHRRGNL 442

Query: 367 YNIHYVVSWG-GDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEYT 426
           YNI Y+V W   D  + +KH++W+R LY YM  +VS +PRGAY+NYRDLDLG+N     T
Sbjct: 443 YNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNYRDLDLGMNR-GVNT 502

Query: 427 SYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLL 465
           S+E A +WG +Y+  NF+RL  +K K+DPT+FF N+QS+P L+
Sbjct: 503 SFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPLI 531

BLAST of MS026413 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 505.4 bits (1300), Expect = 5.1e-143
Identity = 239/465 (51.40%), Postives = 331/465 (71.18%), Query Frame = 0

Query: 4   KTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVS-DVPFVLVDFVNL 63
           +TPKP  I+TP+Q + +Q++I CAR +G  IRTRSGGHDYEG+SY++   PFV++D  NL
Sbjct: 78  ETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNL 137

Query: 64  RSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLR 123
           RSI +DV+ +  WVQSGAT+GELYY+I + SK+L +P G+ PTVG+GG F GGGYG ++R
Sbjct: 138 RSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMR 197

Query: 124 KFGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPVV 183
           K+GL+ DNVIDA++VD NG   DR+ MGED FWAIR GGGSSF ++LSWKI+L+ VP VV
Sbjct: 198 KYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVV 257

Query: 184 TVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSL 243
           TVF + +  E+ A  ++++WQY+A+K  + L IR +L           K   + ASF  L
Sbjct: 258 TVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ----------KETEVYASFPGL 317

Query: 244 FLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFRG 303
           +LG V +LL +M+  FPELG+   +C EMSWIESVL+F     K + +++L  R    R 
Sbjct: 318 YLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFI----KGESMEILAKRKRTSRS 377

Query: 304 VFKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAGN 363
            FKGK D+++EPIPKT +  LW      E + A++ L P+GGKM+EI++ E P+PHR GN
Sbjct: 378 -FKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGN 437

Query: 364 LYNIHYVVSWGGDED----IAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGIN-N 423
           LY I Y+  W  +ED      +K++ W+  +Y +MTP+VSK+PR AYVN+RD+DLG+   
Sbjct: 438 LYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLG 497

Query: 424 INEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIP 462
           +N  T YE+A +WG+KY+K+NF+RLV +KT VDP DFF ++QSIP
Sbjct: 498 LNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MS026413 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.3e-141
Identity = 240/462 (51.95%), Postives = 322/462 (69.70%), Query Frame = 0

Query: 6   PKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV--PFVLVDFVNLR 65
           PKP+ I  PL  +H+QA+++CA+K    +R RSGGHDYEG+SYVS++   FV+VD   LR
Sbjct: 82  PKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLR 141

Query: 66  SIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRK 125
            I +D+E   AWV +GA++GE+YY+I EKSK  G+P G+C ++G+GGH  GG YG M+RK
Sbjct: 142 QISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRK 201

Query: 126 FGLAIDNVIDAYLVDVNGKLHDRKSMGEDVFWAIR-GGGSSFGIILSWKIKLVRVPPVVT 185
           FGL  DNV+DA +VD +GK+ +R +MGEDVFWAIR GGG SFG+IL+WKIKLV VP +VT
Sbjct: 202 FGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVT 261

Query: 186 VFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLF 245
           VFT+ R LE++ T L+++WQ VA+K D+ L IR+I+    +S     K  +I  S+   F
Sbjct: 262 VFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVII--QPTSKTPKSKERTISTSYQGQF 321

Query: 246 LGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFDPKRQPLDVLIDRSFAFRGV 305
           LG    LL +MQR+FP+LG+TK+DC E SWI+SV+Y A F P   P + L+D    F+  
Sbjct: 322 LGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGF-PSTAPSEALLDGKSLFKNY 381

Query: 306 FKGKSDYVDEPIPKTGLIGLWELLNEKEVQFARMHLIPYGGKMNEISELETPYPHRAGNL 365
           FK KSDYV+EPIP  GL GLWE L E++      +  PYGG M +I E ETP+PHR+G L
Sbjct: 382 FKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWN--PYGGMMAKIPETETPFPHRSGTL 441

Query: 366 YNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEYTS 425
           + I ++  W   +    KH+ W+R++Y+YM  +VSK+PR AYVNYRDLDLG+N       
Sbjct: 442 FKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNG-----K 501

Query: 426 YEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLL 465
              A  WG +Y+K NFERLV IK K DP +FF ++QSIP+ L
Sbjct: 502 GSDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152426.12.8e-27698.53berberine bridge enzyme-like 18 [Momordica charantia][more]
XP_022152520.11.4e-21976.58berberine bridge enzyme-like 18 [Momordica charantia][more]
XP_022152423.12.0e-21376.74berberine bridge enzyme-like 15 [Momordica charantia][more]
XP_022152425.15.7e-21376.03berberine bridge enzyme-like 18 [Momordica charantia][more]
XP_022152442.11.1e-20872.42berberine bridge enzyme-like 18 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SVG53.3e-14753.39Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... [more]
Q9FI212.9e-14351.40Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=... [more]
A6P6W03.8e-14351.51Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=... [more]
Q8GTB63.8e-14352.80Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV... [more]
A6P6W15.0e-14351.72Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1DHP31.3e-27698.53berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020162 P... [more]
A0A6J1DE626.8e-22076.58berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 P... [more]
A0A6J1DET99.5e-21476.74berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 P... [more]
A0A6J1DDW62.8e-21376.03berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 P... [more]
A0A6J1DG995.4e-20972.42berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020173 P... [more]
Match NameE-valueIdentityDescription
AT4G20820.12.4e-14853.39FAD-binding Berberine family protein [more]
AT5G44440.12.1e-14451.40FAD-binding Berberine family protein [more]
AT5G44360.13.0e-14352.70FAD-binding Berberine family protein [more]
AT5G44410.15.1e-14351.40FAD-binding Berberine family protein [more]
AT1G30760.11.3e-14151.95FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 403..461
e-value: 3.6E-20
score: 71.8
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 67..461
e-value: 6.9E-168
score: 560.9
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 8..144
e-value: 4.6E-25
score: 88.0
NoneNo IPR availableGENE3D3.40.462.20coord: 180..399
e-value: 6.9E-168
score: 560.9
NoneNo IPR availablePANTHERPTHR32448:SF113BERBERINE BRIDGE ENZYME-LIKE 18coord: 5..463
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 5..463
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 1..62
e-value: 5.9E-26
score: 92.4
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 4..177
score: 17.385683
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 6..178

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS026413.1MS026413.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity