MS026346 (gene) Bitter gourd (TR) v1

BLAST of MS026346 vs. NCBI nr
Match: XP_022153580.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia])

HSP 1 Score: 491.5 bits (1264), Expect = 4.1e-135
Identity = 235/239 (98.33%), Postives = 238/239 (99.58%), Query Frame = 0

Query: 1   MEFEYSLAKAKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDE 60
           MEFEYSLAKAKLPVINLSIENVSPESTNWLEICSH+RAALEEYGCFLGEFGSATPELDDE
Sbjct: 1   MEFEYSLAKAKLPVINLSIENVSPESTNWLEICSHLRAALEEYGCFLGEFGSATPELDDE 60

Query: 61  IFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDL 120
           IFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFV+L
Sbjct: 61  IFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNL 120

Query: 121 MWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP 180
           MWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP
Sbjct: 121 MWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP 180

Query: 181 EMGEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLVY 240
           EMGEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACL +
Sbjct: 181 EMGEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLAW 239

BLAST of MS026346 vs. NCBI nr
Match: XP_022997882.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima])

HSP 1 Score: 388.3 bits (996), Expect = 4.9e-104
Identity = 185/235 (78.72%), Postives = 205/235 (87.23%), Query Frame = 0

Query: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGL 65
           S  K ++P IN SIEN+ P+ST W+E CSHVRAALEEYGCFL E  +   ELDD IF GL
Sbjct: 5   SSKKPEVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECEN-NVELDDAIFNGL 64

Query: 66  EELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPSG 125
           E+LFDLPLETKMKNVSDKPYHGFLDHR PFVLPLH+SFGIEN+ SFE V+SFV LMWPSG
Sbjct: 65  EKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMWPSG 124

Query: 126 NRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEE 185
           N  FCE++F+YTKMVSEMEKLVK MVFESYGV+KYYDSHIASTTYLLR MKYRIPEMGE+
Sbjct: 125 NHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPEMGEQ 184

Query: 186 NIGADAHTDKSFFTILHQNEVNGLEIKT-KDGHWLGVEFTPNSFLVMAGDACLVY 240
           NIGA+AHTDKSFFTILHQN VNGLEIK  KD HW+G++F PNSFLVMAGDACL +
Sbjct: 185 NIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACLAW 238

BLAST of MS026346 vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387.1 bits (993), Expect = 1.1e-103
Identity = 184/238 (77.31%), Postives = 206/238 (86.55%), Query Frame = 0

Query: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATP---ELDDEIF 65
           S    ++PVI+ SIEN+ P+ST W+E CSHVRAALEEYGCFL E+ +      ELDD IF
Sbjct: 5   SSTNPEVPVISFSIENLRPKSTKWVETCSHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64

Query: 66  KGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMW 125
            GLE+LFDLPLETKMKNVSDKPYHGFLDHR PFVLPLH+SFGIEN+ SFE V+SFV LMW
Sbjct: 65  NGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMW 124

Query: 126 PSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEM 185
           PSGN  FCE++F+YTKMVSEMEKLVK MVFESYGV+KYYDSHIASTTYLLR MKYRIPEM
Sbjct: 125 PSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPEM 184

Query: 186 GEENIGADAHTDKSFFTILHQNEVNGLEIKT-KDGHWLGVEFTPNSFLVMAGDACLVY 240
           GE+NIGA+AHTDKSFFTILHQN VNGLEIK  KD HW+G++F PNSFLVMAGDACL +
Sbjct: 185 GEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACLAW 242

BLAST of MS026346 vs. NCBI nr
Match: XP_022948698.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 384.8 bits (987), Expect = 5.5e-103
Identity = 182/238 (76.47%), Postives = 206/238 (86.55%), Query Frame = 0

Query: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATP---ELDDEIF 65
           S  K ++PVIN SIEN+ P+ST W+E C+HVRAALEEYGCFL E+ +      ELDD IF
Sbjct: 5   SSTKPEVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64

Query: 66  KGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMW 125
            GLE+LFDLPL+TKMKNVSDKPYHGFLDHR PFVLPLH+SFGIEN+ SFE V+SFV LMW
Sbjct: 65  NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMW 124

Query: 126 PSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEM 185
           PSGN  FC+++F+YTKMVSEMEKLVK MVFESYGV+KYYDSHIASTTYLLR MKYRIPEM
Sbjct: 125 PSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPEM 184

Query: 186 GEENIGADAHTDKSFFTILHQNEVNGLEIKT-KDGHWLGVEFTPNSFLVMAGDACLVY 240
           GE+NIGA+AHTDKSFFTILHQN VNGLEIK  KD HW+G++F  NSFLVMAGDACL +
Sbjct: 185 GEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNSNSFLVMAGDACLAW 242

BLAST of MS026346 vs. NCBI nr
Match: XP_038878200.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida])

HSP 1 Score: 380.9 bits (977), Expect = 7.9e-102
Identity = 184/239 (76.99%), Postives = 210/239 (87.87%), Query Frame = 0

Query: 6   SLAKAKLPVINLSIEN---VSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIF 65
           S AK+++PVI  SIEN   + P+ST WLE CS VRAALEEYGCFL E+ SA+ +L DEIF
Sbjct: 5   SSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIF 64

Query: 66  KGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMW 125
           KGL+ELFDLPLETKMKNVSDKPYHGFLDH  PFVLPLHQS GIEN+ SF+AV+SF+ L+W
Sbjct: 65  KGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLW 124

Query: 126 PSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEM 185
           PSGN  FCEN+FSY++M+SEME+LVK MVFESYGV KYYDSHIASTTYLLRTMKYRIP+M
Sbjct: 125 PSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQM 184

Query: 186 GEENIGADAHTDKSFFTILHQ-NEVNGLEIKTKDG-HWLGVEFTPNSFLVMAGDACLVY 240
           GEENIGA+AHTDKSFFTILHQ N VNGLEIK K+G HWL ++F+PNSFLVMAGDACL +
Sbjct: 185 GEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAW 243

BLAST of MS026346 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 4.3e-50
Identity = 95/232 (40.95%), Postives = 146/232 (62.93%), Query Frame = 0

Query: 11  KLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGLEELFD 70
           +LPVI+ S +N+ P S+ W E+ + V  ALE+YGCF   F   + EL+  +F+ +E+LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 71  LPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPS-GNRRF 130
           LP+ TK +NVS KP+HG+L H       L++S GI ++   E V  F   +WP  GN+  
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 132

Query: 131 CENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP------EMG 190
            E +  +++ + E++ +V+ M+ ES+G++ Y D H+ ST YL R MKY  P      +  
Sbjct: 133 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 192

Query: 191 EENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDA 236
           E  +G  +HTDK+  TILHQ +V+GLE+KTKD  W+ V+ + +S LVM GD+
Sbjct: 193 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDS 238

BLAST of MS026346 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 7.3e-50
Identity = 95/231 (41.13%), Postives = 147/231 (63.64%), Query Frame = 0

Query: 11  KLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGLEELFD 70
           +LPVI+ S +N+ P S+ W E+ + V  ALE+YGCF   F   + EL+  +F+ +E+LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 72

Query: 71  LPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPS-GNRRF 130
           LP+ TK +NVS KP+HG+L H       L++S GI+++   E V  F   +WP  GN+  
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQLWPDHGNKSI 132

Query: 131 CENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP-----EMGE 190
            E +  +++ + E++ +V+ M+ ES+G++ Y D H+ ST YL R MKY  P     +  E
Sbjct: 133 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEE 192

Query: 191 ENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDA 236
             +G  +HTDK+  TILHQ +V+GLE+KTKD  W+ V+ + +S LVM GD+
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDS 237

BLAST of MS026346 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 9.5e-50
Identity = 95/230 (41.30%), Postives = 146/230 (63.48%), Query Frame = 0

Query: 11  KLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGLEELFD 70
           +LPVI+ S +N+ P S+ W E+ + V  ALE+YGCF   F   + EL+  +F+ +E+LF+
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 71  LPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPS-GNRRF 130
           LP+ TK +NVS K +HG+L H       L++S GI ++   E V  F   +WP  GN+  
Sbjct: 66  LPIPTKQRNVSSKLFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 125

Query: 131 CENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP----EMGEE 190
            E +  +++ + E++ +V+ M+ ES+G++KY D H+ ST YL R MKY  P    +  E 
Sbjct: 126 SETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEET 185

Query: 191 NIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDA 236
            +G  +HTDK+  TILHQ +V+GLE+KTKD  W+ V+ + +S LVM GD+
Sbjct: 186 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDS 229

BLAST of MS026346 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 4.7e-49
Identity = 96/234 (41.03%), Postives = 144/234 (61.54%), Query Frame = 0

Query: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGL 65
           S A  +LPVI+LS++N+ P +T+W  + + VR ALEEYGCF     + +PEL   +    
Sbjct: 3   STAPLRLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKG 62

Query: 66  EELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPSG 125
            EL +LPLETKM N +   Y GF       +  L++  G+      E V+ F +LMWP G
Sbjct: 63  HELLNLPLETKMLNGNKPEYDGFTS-----IPNLNEGMGVGRITDLEKVERFTNLMWPEG 122

Query: 126 NRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEE 185
           N+ FCE V+SY K ++E++ ++K+MVFES+G++K++DS   ST YLL  M+Y+ P     
Sbjct: 123 NKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGR 182

Query: 186 NIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLVY 240
           +     H DKS  TI++QN+V GLE +TKDG W  +  T ++ +V+ GD  + +
Sbjct: 183 SPALSLHKDKSILTIVNQNDVKGLEFETKDGEW--ILPTADNHIVLLGDCFMAW 229

BLAST of MS026346 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 7.5e-39
Identity = 98/343 (28.57%), Postives = 146/343 (42.57%), Query Frame = 0

Query: 11  KLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGLEELFD 70
           +LP+INL+ + + P S+ W E+ S VR ALE++GCF   +   + EL + I K +EELF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFA 66

Query: 71  LPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWP--SGNRR 130
           LP+ETK +NV  KPY G+L+H       L +S GI N+   E V  F   +WP   GN  
Sbjct: 67  LPVETKQRNVCPKPYVGYLNHNN-----LSESLGISNANILENVNEFTQQLWPDGDGNEN 126

Query: 131 FCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEEN-- 190
             + +  + + + E++ +V+ MV ES+G++KY D H+ ST Y +R MKY  P  G+ N  
Sbjct: 127 ISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDANTT 186

Query: 191 ------------------------------------------------------------ 236
                                                                       
Sbjct: 187 VDDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVDA 246

BLAST of MS026346 vs. ExPASy TrEMBL
Match: A0A6J1DH70 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN=LOC111021051 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.0e-135
Identity = 235/239 (98.33%), Postives = 238/239 (99.58%), Query Frame = 0

Query: 1   MEFEYSLAKAKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDE 60
           MEFEYSLAKAKLPVINLSIENVSPESTNWLEICSH+RAALEEYGCFLGEFGSATPELDDE
Sbjct: 1   MEFEYSLAKAKLPVINLSIENVSPESTNWLEICSHLRAALEEYGCFLGEFGSATPELDDE 60

Query: 61  IFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDL 120
           IFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFV+L
Sbjct: 61  IFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNL 120

Query: 121 MWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP 180
           MWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP
Sbjct: 121 MWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP 180

Query: 181 EMGEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLVY 240
           EMGEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACL +
Sbjct: 181 EMGEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLAW 239

BLAST of MS026346 vs. ExPASy TrEMBL
Match: A0A6J1K6A3 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LOC111492707 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.4e-104
Identity = 185/235 (78.72%), Postives = 205/235 (87.23%), Query Frame = 0

Query: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGL 65
           S  K ++P IN SIEN+ P+ST W+E CSHVRAALEEYGCFL E  +   ELDD IF GL
Sbjct: 5   SSKKPEVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECEN-NVELDDAIFNGL 64

Query: 66  EELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPSG 125
           E+LFDLPLETKMKNVSDKPYHGFLDHR PFVLPLH+SFGIEN+ SFE V+SFV LMWPSG
Sbjct: 65  EKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMWPSG 124

Query: 126 NRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEE 185
           N  FCE++F+YTKMVSEMEKLVK MVFESYGV+KYYDSHIASTTYLLR MKYRIPEMGE+
Sbjct: 125 NHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPEMGEQ 184

Query: 186 NIGADAHTDKSFFTILHQNEVNGLEIKT-KDGHWLGVEFTPNSFLVMAGDACLVY 240
           NIGA+AHTDKSFFTILHQN VNGLEIK  KD HW+G++F PNSFLVMAGDACL +
Sbjct: 185 NIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACLAW 238

BLAST of MS026346 vs. ExPASy TrEMBL
Match: A0A6J1GA17 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111452291 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.6e-103
Identity = 182/238 (76.47%), Postives = 206/238 (86.55%), Query Frame = 0

Query: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATP---ELDDEIF 65
           S  K ++PVIN SIEN+ P+ST W+E C+HVRAALEEYGCFL E+ +      ELDD IF
Sbjct: 5   SSTKPEVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64

Query: 66  KGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMW 125
            GLE+LFDLPL+TKMKNVSDKPYHGFLDHR PFVLPLH+SFGIEN+ SFE V+SFV LMW
Sbjct: 65  NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMW 124

Query: 126 PSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEM 185
           PSGN  FC+++F+YTKMVSEMEKLVK MVFESYGV+KYYDSHIASTTYLLR MKYRIPEM
Sbjct: 125 PSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPEM 184

Query: 186 GEENIGADAHTDKSFFTILHQNEVNGLEIKT-KDGHWLGVEFTPNSFLVMAGDACLVY 240
           GE+NIGA+AHTDKSFFTILHQN VNGLEIK  KD HW+G++F  NSFLVMAGDACL +
Sbjct: 185 GEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNSNSFLVMAGDACLAW 242

BLAST of MS026346 vs. ExPASy TrEMBL
Match: A0A0A0LBU1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G555690 PE=4 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.5e-90
Identity = 176/254 (69.29%), Postives = 205/254 (80.71%), Query Frame = 0

Query: 7   LAKAKLPVINLS-----IENVSPESTN-WLEICSHVRAALEEYGCFLGEF---------- 66
           LAK ++P+I LS      ++  PES   WLE    VR ALEEYGCFLGE+          
Sbjct: 6   LAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCN 65

Query: 67  GSATPELD----DEIFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIE 126
            S++P+L+    +EIFKGL+ELFDLPLETK+KNVSDKPYHGFLD RTPFVLPLHQS GIE
Sbjct: 66  SSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIE 125

Query: 127 NSESFEAVQSFVDLMWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIA 186
           N+ S+EAVQ FV+L+WPSGN  FCE +F+Y+K +SEME+LVK MVFESYGV KYYDSHIA
Sbjct: 126 NASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIA 185

Query: 187 STTYLLRTMKYRIPEMGEENIGADAHTDKSFFTILHQ-NEVNGLEIKTKD-GHWLGVEFT 239
           STTYLLRTMKYRIP+MGEENIGA+AHTDKSFFTILHQ + VNGL+IK K+   WLGV+F+
Sbjct: 186 STTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFS 245

BLAST of MS026346 vs. ExPASy TrEMBL
Match: A0A6P3YXF4 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Ziziphus jujuba OX=326968 GN=LOC107406140 PE=3 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 1.2e-76
Identity = 137/233 (58.80%), Postives = 174/233 (74.68%), Query Frame = 0

Query: 10  AKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGLEELF 69
           AKLP I+ S EN+ P ++ W       R ALE+YGCF+  +   +P+L D IF+ L+ELF
Sbjct: 7   AKLPAIDFSKENLKPGTSCWSSTAIDARQALEQYGCFVAIYDKMSPQLHDSIFRELKELF 66

Query: 70  DLPLETKMKNVSDKPYHGFL---DHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPSGN 129
           DLP E KMKN+SDKPY G+       TP V  LH+S GIEN+ + EAVQSF +LMWP+GN
Sbjct: 67  DLPTEIKMKNISDKPYFGYAAGNSATTPHVPSLHESLGIENTTTLEAVQSFTNLMWPAGN 126

Query: 130 RRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEEN 189
             FCE V SYTK+V+E+E++VK MVFESYG +KYYDSH+ STTYLLR +KYR PE+ E +
Sbjct: 127 HSFCETVLSYTKLVAELEQMVKRMVFESYGAEKYYDSHVGSTTYLLRQIKYRAPEIDESD 186

Query: 190 IGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLVY 240
           +G + HTDKSF TILHQNEV G E+KTKDG+W+G E +P+SFLV+AGDA L +
Sbjct: 187 VGCEIHTDKSFITILHQNEVEGFELKTKDGYWIGFEPSPSSFLVLAGDAFLAW 239

BLAST of MS026346 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.0e-62
Identity = 107/229 (46.72%), Postives = 152/229 (66.38%), Query Frame = 0

Query: 7   LAKAKLPVINL-SIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGL 66
           L   K+P+++L S + + P ++ W  +      ALEEYGCFL  +   T +LDD IF   
Sbjct: 5   LKTTKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAA 64

Query: 67  EELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPSG 126
           EELFDLP ETK KNV++KPYHG++      V+PLH+  G++   + E  Q F  LMWP G
Sbjct: 65  EELFDLPTETKKKNVNEKPYHGYVGQMP--VIPLHEGLGVDYVTNKEIAQRFTHLMWPQG 124

Query: 127 NRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEE 186
           N RFC  V +++  V+E+++LV  M+FE+YGV+K+Y+SH+ S TYLL+ +KY  P     
Sbjct: 125 NDRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESIS 184

Query: 187 NIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGD 235
                 HTDK+F +ILHQN+VNGLE+K+KDG W+ ++  P S++VMAGD
Sbjct: 185 MPAFPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSYVVMAGD 231

BLAST of MS026346 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 221.9 bits (564), Expect = 5.7e-58
Identity = 107/231 (46.32%), Postives = 156/231 (67.53%), Query Frame = 0

Query: 11  KLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATP-ELDDEIFKGLEELF 70
           K+P ++ S E++ P +  W     ++R ALEEYGCF+ +    TP +L D +F  L +LF
Sbjct: 4   KIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 63

Query: 71  DLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPSGNRRF 130
           DLP +TKMKN  DKP +G++  + P  LPLH+S GI+N+ S EA +SF  LMWP GN  F
Sbjct: 64  DLPTQTKMKNKYDKPLNGYVG-QIP-ALPLHESLGIDNATSLEATRSFTGLMWPQGNEHF 123

Query: 131 CENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEENIGA 190
            E ++ Y +  +E++++V  MVF+SY V+KYYD +I STTYLLR +K R P      +G 
Sbjct: 124 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGF 183

Query: 191 DAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEF-TPNSFLVMAGDACLVY 240
             HTDKSF TILHQ++VNGLE++T++G  + +   +P+ F+V+AGDA + +
Sbjct: 184 VTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAW 232

BLAST of MS026346 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 214.9 bits (546), Expect = 7.0e-56
Identity = 103/235 (43.83%), Postives = 153/235 (65.11%), Query Frame = 0

Query: 1   MEFEYSLAKAKLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDE 60
           M  E  L   +LPVI+ S +N+ P    W    + V+ AL++YG F   F     EL   
Sbjct: 1   MGSETPLLPLRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKS 60

Query: 61  IFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDL 120
           +F  LEELFDLPL+TK++NVS KP+HG++      ++PL++S GI++S+  E V +F + 
Sbjct: 61  VFGALEELFDLPLQTKLRNVSKKPFHGYVGQYP--MVPLYESMGIDDSDIAEKVDAFTEK 120

Query: 121 MWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP 180
           +WP GN  F   + S++K +SE++  ++ M+ ES+G+ KY D H+ ST YLLR MKY+ P
Sbjct: 121 LWPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGP 180

Query: 181 EMGEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDA 236
           +  E  +G +AHTDK+  TIL+QN V GLE++TKD +W+ V+ T +SF VM GD+
Sbjct: 181 DTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDS 233

BLAST of MS026346 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 199.5 bits (506), Expect = 3.0e-51
Identity = 95/232 (40.95%), Postives = 146/232 (62.93%), Query Frame = 0

Query: 11  KLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGLEELFD 70
           +LPVI+ S +N+ P S+ W E+ + V  ALE+YGCF   F   + EL+  +F+ +E+LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 71  LPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWPS-GNRRF 130
           LP+ TK +NVS KP+HG+L H       L++S GI ++   E V  F   +WP  GN+  
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 132

Query: 131 CENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIP------EMG 190
            E +  +++ + E++ +V+ M+ ES+G++ Y D H+ ST YL R MKY  P      +  
Sbjct: 133 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 192

Query: 191 EENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDA 236
           E  +G  +HTDK+  TILHQ +V+GLE+KTKD  W+ V+ + +S LVM GD+
Sbjct: 193 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDS 238

BLAST of MS026346 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 5.5e-45
Identity = 94/233 (40.34%), Postives = 137/233 (58.80%), Query Frame = 0

Query: 11  KLPVINLSIENVSPESTNWLEICSHVRAALEEYGCFLGEFGSATPELDDEIFKGLEELFD 70
           +LP+INLS +N+   +  W      VR A+E +G F+ E+ +    L   I +  +EL D
Sbjct: 8   ELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKELLD 67

Query: 71  LPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVDLMWP---SGNR 130
           LP+E K+KN + K  HG++   +    P+H+  GI+     +  + F  LMWP     N 
Sbjct: 68  LPVEIKLKNENHKAGHGYITMMSD-GQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDDND 127

Query: 131 RFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEENI 190
           RFCE V +Y KM +E+E+LV  M+FESY V+KY + +I  T YLLR +KYR    GE N 
Sbjct: 128 RFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPNGEPNR 187

Query: 191 GADAHTDKSFFTILHQNEVNGLEIKT-KDGHWLGVEFTPNSFLVMAGDACLVY 240
              +HTDKSF +ILHQN + GL +K+ K+  W     +P  F+V+AGDA + +
Sbjct: 188 KFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSPTRFVVIAGDAIMAW 239