MS025316 (gene) Bitter gourd (TR) v1

Overview
NameMS025316
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCASP-like protein
Locationscaffold25: 1104653 .. 1107087 (+)
RNA-Seq ExpressionMS025316
SyntenyMS025316
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTTTTTGGGGATCTAAGAACTGAAGGGATTCTCAGATTCTGTGCGATTATTGGCCTTATTCTAACAGTTTGTTTGCTGGGTTTTGATAAACAAACTACTCAGATCTTTCATGTCGACAAAAAGGCCACTTTCAGAAGTTTGCGTTCTCTCATGTAAGTTTTCTCCCTTTAAATTTTATCTTTTACCAGTCGAGTTATACTCAAATTAGCAACGCTTCTATGGTTTGGTTCTCGCCGATAAAGCATTAATATTTCCAAATTTTATATATATGAATTGAACGAGTTAATATTAGTTAGAAATTAAGATTGAAGGTTCGATTCTCTACTCCACAATGATTGGACTAGAAAAAGTTAAAGCGTAACTCAACTAGCTAGTTGAAGTATTTTGAAAGAAAAAATCGAATAAACAGTCATATTGTATTTTTCTTTTTCTTTTTCTTTTACGAAACATATTGTTAATGATTTGTGGTTGTTTTTGGCGACAGAGTGATTATGTATGTGAACTCAGTGGCAGCTGGGTACAATCTTCTTCAACTCTGCAAGTGTTGGATCTCAGCTCAGCCAAAATTAGGAGCCTCAAAATTAGGGGCTCACTATGATATTTACATATTTTGGATTTCTTTCTTCCTTGATCAGGTATGTCTCTTCAATTAATTTAGATGGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTNAATTACGTGAATGAGTTTTCCTAACTTTTATTTGGTTCATGAGTTTATTGATTTGGAAAAATTTATGAGAAAAAATCCATTACCTTCCATTTCTAAAATGTTGGTTAATTTGTATTTTTAATAAAAAAAAAGTTTAAAATATAGTTGGTTTTTTAAGTTCTTAATCATTGATTAACTTAATAATAATAATAATAATTTGTTTTCGGTTTTAACTTTTGATGATATTGGTTAGCTATGATGATCAGTCATATTGATTGAGATGACTATGGCCAGTTTATTTTATTATCAAACTTTAGAAGAAGTCAGAAATTAAAAATTGAAGCAAAATGTTTTAATATCTCTAGTTTCCCTCACCAAACAAACTTGTGGCCATAATTGCCACACATTTCGGAAAGTTCTAGGTTGGGTTTAGAGATTCTTTTTTCTTTTAACCTAATCCAATACTTCTTCATATAGTTATAAATTTTTCCAATTCAATTAGGATTGATTTTTTCAAATCATTGCTTCATATCATAAATTTAATATATATTTTAAACGAACATCACAATTTTCATCATTTTATTTAGGGCTCGTTTGGTAACTTTCCTGTTTTTTGTTTCTGTTTCTTTATTTTTAATGAAAAAAAACGTGTTGCATTGTTTTAACCTCCAAAAATTCAAATATGATAAAACAATGTTTTTATTTATATTTTGCAATAAATTGGAAATGGGAACCGGAAGAGAGAAACTTGTTTTGATTGTTCCCAAAATTTCTATACAATTGTGAGAATGTTCCAAAAAAGGAGAAACAAGAAACAAAGTTAGTTGTAAAATACCTAGATTTCTTAAGAAAATTGAAAACGAGAAACAAGAAATCGGAACGTTACCAAACAGGCCCTTATAGAATAAGCGTGACTTATTTTTCTTTTTTTTTTTATTTTACGTTATATTTGAGTTTAATTTTAATTTGAGTTTTTTTTTATTTTAAATTTTATGTTATATTTAAAAATAGATATTTATTTTAAGTTATTGATGGCTTCTTTTCCAAGAGAAAAATGAAAAACAAAAAATAAAAGTAATGTAGAGTTCATTGGATGACCTTTTTGTTTTTAAAAAACCAAAGCCAACATAAACTTAATTTAATGGTAATTGACATCTGTCTCTAATAAAAAATTATGAATTCGAATCTCCACGTCATCCTTTGGAAGAATAAAAAACCAGTATAAATTAGGGTGATAATCAACCAGACGCAGGTGAGTATAACTATCCCAAGTTAAGATATAAGTAATCTTTCACATCCTCAATCTATCCCCTTGCTTGTAATAGTCATCAACGGGTCCATAAACGCTACCAAACAAGTGCAGAAAGGTTTTAATGGTGTTCCACCTTACAAGTTGGGAGAGGTTTAAAGGTTGGGTTGAACTGGATTTTGACACAGGCAAGTGCATATGTGATATTTGCGGCGAACACGGCGGGGGTGGAGGCGGCTCTTCTGGCGATCACCGGAGCCTACAACCTTCAATGGATGAAGCTGTGCAACAGATTCACCAGATTTTGCTTCCAAGCTGGTGGAGCTTTCCTTTGTGGCTATGCAGCTTCTCTTACAATGGCTGCCATTTCCTTCATTTCTGCTTTCAATTTGTTCACCCATTACTCTCCAAGCCACTTCCTGCGACCAAAATCCAAGTGA

mRNA sequence

ATGGGGTTTTTTGGGGATCTAAGAACTGAAGGGATTCTCAGATTCTGTGCGATTATTGGCCTTATTCTAACAGTTTGTTTGCTGGGTTTTGATAAACAAACTACTCAGATCTTTCATGTCGACAAAAAGGCCACTTTCAGAAGTTTGCGTTCTCTCATAGTGATTATGTATGTGAACTCAGTGGCAGCTGGGTACAATCTTCTTCAACTCTGCAAGTGTTGGATCTCAGCTCAGCCAAAATTAGGAGCCTCAAAATTAGGGGCTCACTATGATATTTACATATTTTGGATTTCTTTCTTCCTTGATCAGGCAAGTGCATATGTGATATTTGCGGCGAACACGGCGGGGGTGGAGGCGGCTCTTCTGGCGATCACCGGAGCCTACAACCTTCAATGGATGAAGCTGTGCAACAGATTCACCAGATTTTGCTTCCAAGCTGGTGGAGCTTTCCTTTGTGGCTATGCAGCTTCTCTTACAATGGCTGCCATTTCCTTCATTTCTGCTTTCAATTTGTTCACCCATTACTCTCCAAGCCACTTCCTGCGACCAAAATCCAAGTGA

Coding sequence (CDS)

ATGGGGTTTTTTGGGGATCTAAGAACTGAAGGGATTCTCAGATTCTGTGCGATTATTGGCCTTATTCTAACAGTTTGTTTGCTGGGTTTTGATAAACAAACTACTCAGATCTTTCATGTCGACAAAAAGGCCACTTTCAGAAGTTTGCGTTCTCTCATAGTGATTATGTATGTGAACTCAGTGGCAGCTGGGTACAATCTTCTTCAACTCTGCAAGTGTTGGATCTCAGCTCAGCCAAAATTAGGAGCCTCAAAATTAGGGGCTCACTATGATATTTACATATTTTGGATTTCTTTCTTCCTTGATCAGGCAAGTGCATATGTGATATTTGCGGCGAACACGGCGGGGGTGGAGGCGGCTCTTCTGGCGATCACCGGAGCCTACAACCTTCAATGGATGAAGCTGTGCAACAGATTCACCAGATTTTGCTTCCAAGCTGGTGGAGCTTTCCTTTGTGGCTATGCAGCTTCTCTTACAATGGCTGCCATTTCCTTCATTTCTGCTTTCAATTTGTTCACCCATTACTCTCCAAGCCACTTCCTGCGACCAAAATCCAAGTGA

Protein sequence

MGFFGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRPKSK
Homology
BLAST of MS025316 vs. NCBI nr
Match: XP_022153293.1 (CASP-like protein 2C1 [Momordica charantia])

HSP 1 Score: 369.4 bits (947), Expect = 1.8e-98
Identity = 184/186 (98.92%), Postives = 184/186 (98.92%), Query Frame = 0

Query: 1   MGFFGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS 60
           MGFFGD RTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS
Sbjct: 1   MGFFGDPRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS 60

Query: 61  VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAA 120
           VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQA AYVIFAANTAGVEAA
Sbjct: 61  VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQAIAYVIFAANTAGVEAA 120

Query: 121 LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF 180
           LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF
Sbjct: 121 LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF 180

Query: 181 LRPKSK 187
           LRPKSK
Sbjct: 181 LRPKSK 186

BLAST of MS025316 vs. NCBI nr
Match: XP_038889630.1 (CASP-like protein 2C1 [Benincasa hispida])

HSP 1 Score: 288.5 bits (737), Expect = 4.1e-74
Identity = 135/182 (74.18%), Postives = 162/182 (89.01%), Query Frame = 0

Query: 4   FGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAA 63
           FG++RTEGILRFCAI  L+L+VCLLGFDKQTTQIFH+DKKA+FRSLR+L++I+YV+S+ A
Sbjct: 3   FGNVRTEGILRFCAIFLLVLSVCLLGFDKQTTQIFHIDKKASFRSLRALVIIIYVDSMVA 62

Query: 64  GYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLA 123
           GYN+LQ+CKCWI AQPK G SKLG H+DIY+FW+SFFLDQA+ Y+ FAANTAG+EA+ LA
Sbjct: 63  GYNILQICKCWIFAQPK-GISKLGGHFDIYLFWLSFFLDQAAVYLTFAANTAGMEASFLA 122

Query: 124 ITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRP 183
           +TGA + QWMKLCNRF RFC+Q GGAFLCGYAA  T+  ISFISAF+LF HYSP+HFLR 
Sbjct: 123 VTGARDFQWMKLCNRFHRFCYQVGGAFLCGYAAFSTLLVISFISAFSLFRHYSPNHFLRL 182

Query: 184 KS 186
           KS
Sbjct: 183 KS 183

BLAST of MS025316 vs. NCBI nr
Match: XP_004149074.1 (CASP-like protein 2C1 [Cucumis sativus] >KGN61092.1 hypothetical protein Csa_021217 [Cucumis sativus])

HSP 1 Score: 286.2 bits (731), Expect = 2.0e-73
Identity = 135/182 (74.18%), Postives = 162/182 (89.01%), Query Frame = 0

Query: 4   FGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAA 63
           FGD++TEGILRFCAII L+L+V LLGFDK+T QIFHVDKKA+F+SLR+L++I+YV+S+AA
Sbjct: 3   FGDVKTEGILRFCAIILLVLSVLLLGFDKETVQIFHVDKKASFKSLRALVIIIYVDSMAA 62

Query: 64  GYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLA 123
           GYN+LQLCKCWI AQPK G SKLG H+ IY+FW+SFFLDQ +AY+ FA+NTAG+EAALLA
Sbjct: 63  GYNILQLCKCWIFAQPK-GISKLGTHFHIYLFWLSFFLDQVAAYLTFASNTAGMEAALLA 122

Query: 124 ITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRP 183
           +TGA++ QWMKLCNRF RFC+Q GGAFLCGYAA   +  ISFISAFNLF HYSP+HFLR 
Sbjct: 123 VTGAHDFQWMKLCNRFHRFCYQVGGAFLCGYAAFFALLLISFISAFNLFRHYSPNHFLRL 182

Query: 184 KS 186
           KS
Sbjct: 183 KS 183

BLAST of MS025316 vs. NCBI nr
Match: XP_023537147.1 (CASP-like protein 2C1 [Cucurbita pepo subsp. pepo] >KAG6586191.1 CASP-like protein 2C1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021022.1 CASP-like protein 2C1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 265.0 bits (676), Expect = 4.9e-67
Identity = 133/185 (71.89%), Postives = 154/185 (83.24%), Query Frame = 0

Query: 1   MGFFGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS 60
           MGF+ D RTEGILR  AIIGL+LTV LLGFDKQTTQIFH+DKKA+FRSL + +VIMYV+S
Sbjct: 1   MGFW-DQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDS 60

Query: 61  VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAA 120
           +AA YN+LQ+CKCWISAQPK G SKLG+   I +FW SF LDQA AY++FAANTAG+EAA
Sbjct: 61  LAAAYNILQICKCWISAQPK-GTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAA 120

Query: 121 LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF 180
           LLA+TGA++ QWMKLCNRF RFC+Q GGAFLCGY A   M AI  IS+FNLF HYSP+ F
Sbjct: 121 LLAVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLF 180

Query: 181 LRPKS 186
            R KS
Sbjct: 181 FRLKS 183

BLAST of MS025316 vs. NCBI nr
Match: XP_022938059.1 (CASP-like protein 2C1 [Cucurbita moschata])

HSP 1 Score: 263.1 bits (671), Expect = 1.8e-66
Identity = 132/185 (71.35%), Postives = 153/185 (82.70%), Query Frame = 0

Query: 1   MGFFGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS 60
           MGF+ D RTEGILR  AIIGL+LTV LLGFDKQTTQIFH+DKKA+FRSL + +VIMYV+S
Sbjct: 1   MGFW-DQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDS 60

Query: 61  VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAA 120
           +AA YN+LQ+CKCWISA PK G SKLG+   I +FW SF LDQA AY++FAANTAG+EAA
Sbjct: 61  LAAAYNILQICKCWISAHPK-GTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAA 120

Query: 121 LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF 180
           LLA+TGA++ QWMKLCNRF RFC+Q GGAFLCGY A   M AI  IS+FNLF HYSP+ F
Sbjct: 121 LLAVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLF 180

Query: 181 LRPKS 186
            R KS
Sbjct: 181 FRLKS 183

BLAST of MS025316 vs. ExPASy Swiss-Prot
Match: B9IM09 (CASP-like protein 2C1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_578614 PE=3 SV=2)

HSP 1 Score: 179.9 bits (455), Expect = 2.7e-44
Identity = 94/177 (53.11%), Postives = 120/177 (67.80%), Query Frame = 0

Query: 8   RTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAAGYNL 67
           + E ILR  AI+ L+ T CL+G D QT  +   +K+ T++ L +L+V++YV++VAA YNL
Sbjct: 7   KIEAILRGIAILLLVSTACLVGLDSQTKFVIVYEKEVTYKDLHALVVLVYVDAVAAAYNL 66

Query: 68  LQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLAITGA 127
           LQLC+C +SA   L        Y  Y+ W  F LDQ +AY  FAA++A ++ ++L ITGA
Sbjct: 67  LQLCRCSVSA---LSKGNFKGSYR-YLSWACFVLDQLAAYTTFAAHSAALQHSVLGITGA 126

Query: 128 YNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRPK 185
              QWMK CNRFTRFCFQ GGA  CGY AS+ M  ISFISAFNLF  YSP HFLR K
Sbjct: 127 KVFQWMKWCNRFTRFCFQIGGALTCGYIASVLMVMISFISAFNLFRLYSPKHFLRLK 179

BLAST of MS025316 vs. ExPASy Swiss-Prot
Match: A7Q6G6 (CASP-like protein 2C1 OS=Vitis vinifera OX=29760 GN=VIT_11s0052g01140 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.3e-43
Identity = 97/179 (54.19%), Postives = 126/179 (70.39%), Query Frame = 0

Query: 8   RTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKK-ATFRSLRSLIVIMYVNSVAAGYN 67
           R E  LR  AI+ L+LT CLLGFD QT  +F   KK ATFR L +L V++YV+SVAAGYN
Sbjct: 30  RAEAFLRLFAILVLVLTACLLGFDTQTKLLFSTIKKTATFRDLGALQVVVYVDSVAAGYN 89

Query: 68  LLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLAITG 127
           LLQL + +ISA  KL    +   Y + + W+ F LDQA+ Y +F+ANTA ++A+++A+TG
Sbjct: 90  LLQLGRGFISA--KLKGKLINVSY-VTLPWVCFLLDQAAVYTVFSANTAALQASIIAVTG 149

Query: 128 AYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPS--HFLRP 184
             +LQWMK+CNR+TRFC Q GGA L GY ASL M  +S +SAF+LF  YSP   H L+P
Sbjct: 150 ESSLQWMKVCNRYTRFCIQVGGALLSGYLASLLMVLLSSLSAFSLFRLYSPKQFHLLKP 205

BLAST of MS025316 vs. ExPASy Swiss-Prot
Match: Q5K4H9 (CASP-like protein XL3 OS=Gossypium hirsutum OX=3635 GN=XL3 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.3e-41
Identity = 85/177 (48.02%), Postives = 119/177 (67.23%), Query Frame = 0

Query: 8   RTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAAGYNL 67
           + E ++R   I+ L+LT CL+G D QT  IF+V KKA+F+ LR+L+ ++Y+ S+AA YNL
Sbjct: 7   KIEALIRLSTIVMLVLTACLIGLDSQTKVIFYVQKKASFKDLRALVGLLYITSLAAAYNL 66

Query: 68  LQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLAITGA 127
           LQLC    SA  K   + L ++   Y+ W+ + LDQA  Y +FA N A +E + L +TG 
Sbjct: 67  LQLCCSSFSASYK--GTSLQSY--AYLAWLRYILDQAVVYAVFAGNLAALEHSFLVLTGE 126

Query: 128 YNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRPK 185
            N QW+K CN++TRFC Q GG+ LCG+ ASL M +I+ ISAFNLF  YSP+ F+  K
Sbjct: 127 ENFQWLKWCNKYTRFCTQIGGSLLCGFVASLLMFSIASISAFNLFRQYSPTKFMHLK 179

BLAST of MS025316 vs. ExPASy Swiss-Prot
Match: C6SZ04 (CASP-like protein 2C1 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.4e-39
Identity = 91/182 (50.00%), Postives = 120/182 (65.93%), Query Frame = 0

Query: 5   GDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIF-HVDKKATFRSLRSLIVIMYVNSVAA 64
           G  R E  LR  AI+ L+LT CL+  D QT  +F  ++KKAT++ L +L +++YV   AA
Sbjct: 4   GMARGEVCLRVSAILVLVLTACLVALDTQTKVVFVSIEKKATYKDLNALKILVYVTCAAA 63

Query: 65  GYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLA 124
           GYNLL LCK  I ++     S L       + WI F LDQ + Y+ FAANTA + AA+LA
Sbjct: 64  GYNLLLLCKHSIWSRKNFKGSYL------CMAWICFSLDQIAVYMTFAANTATMGAAVLA 123

Query: 125 ITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRP 184
           I+G+   QW+K+C++FTRFC + GGA LCGYAAS+ MA IS ISA+ +F  YSP  FLR 
Sbjct: 124 ISGSEAFQWLKVCDKFTRFCVEIGGALLCGYAASMLMALISTISAYKVFRMYSPKWFLRL 179

Query: 185 KS 186
           KS
Sbjct: 184 KS 179

BLAST of MS025316 vs. ExPASy Swiss-Prot
Match: Q8L8U9 (CASP-like protein 2C1 OS=Arabidopsis thaliana OX=3702 GN=At4g25830 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 4.6e-36
Identity = 80/177 (45.20%), Postives = 106/177 (59.89%), Query Frame = 0

Query: 9   TEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAAGYNLL 68
           TE ILR C +  L+LT CL+G D QT +I ++ K  +FR L +L   +Y++ V A YNL+
Sbjct: 7   TEVILRLCIVFFLLLTSCLIGLDSQTKEIAYIHKNVSFRYLLALEAELYIDVVVAAYNLV 66

Query: 69  QLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLAITGAY 128
           QL   W + + K    K          W S+ LDQ +AYV+FA  +A  + +LL +TG+ 
Sbjct: 67  QLGLGWYNVEQKTSNPK----------WFSYLLDQTAAYVVFAGTSAAAQHSLLVVTGSR 126

Query: 129 NLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRPKS 186
            LQWMK C +FTRFCFQ G A +  Y A+  M  +S ISAFNLF  YSP  F R KS
Sbjct: 127 ELQWMKWCYKFTRFCFQMGSAIILNYIAAALMVLLSSISAFNLFRLYSPKRFFRFKS 173

BLAST of MS025316 vs. ExPASy TrEMBL
Match: A0A6J1DH36 (CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111020824 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 8.9e-99
Identity = 184/186 (98.92%), Postives = 184/186 (98.92%), Query Frame = 0

Query: 1   MGFFGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS 60
           MGFFGD RTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS
Sbjct: 1   MGFFGDPRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS 60

Query: 61  VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAA 120
           VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQA AYVIFAANTAGVEAA
Sbjct: 61  VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQAIAYVIFAANTAGVEAA 120

Query: 121 LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF 180
           LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF
Sbjct: 121 LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF 180

Query: 181 LRPKSK 187
           LRPKSK
Sbjct: 181 LRPKSK 186

BLAST of MS025316 vs. ExPASy TrEMBL
Match: A0A0A0LK62 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G048830 PE=3 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 9.9e-74
Identity = 135/182 (74.18%), Postives = 162/182 (89.01%), Query Frame = 0

Query: 4   FGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAA 63
           FGD++TEGILRFCAII L+L+V LLGFDK+T QIFHVDKKA+F+SLR+L++I+YV+S+AA
Sbjct: 3   FGDVKTEGILRFCAIILLVLSVLLLGFDKETVQIFHVDKKASFKSLRALVIIIYVDSMAA 62

Query: 64  GYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLA 123
           GYN+LQLCKCWI AQPK G SKLG H+ IY+FW+SFFLDQ +AY+ FA+NTAG+EAALLA
Sbjct: 63  GYNILQLCKCWIFAQPK-GISKLGTHFHIYLFWLSFFLDQVAAYLTFASNTAGMEAALLA 122

Query: 124 ITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRP 183
           +TGA++ QWMKLCNRF RFC+Q GGAFLCGYAA   +  ISFISAFNLF HYSP+HFLR 
Sbjct: 123 VTGAHDFQWMKLCNRFHRFCYQVGGAFLCGYAAFFALLLISFISAFNLFRHYSPNHFLRL 182

Query: 184 KS 186
           KS
Sbjct: 183 KS 183

BLAST of MS025316 vs. ExPASy TrEMBL
Match: A0A6J1FCZ2 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444261 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 9.0e-67
Identity = 132/185 (71.35%), Postives = 153/185 (82.70%), Query Frame = 0

Query: 1   MGFFGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS 60
           MGF+ D RTEGILR  AIIGL+LTV LLGFDKQTTQIFH+DKKA+FRSL + +VIMYV+S
Sbjct: 1   MGFW-DQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDS 60

Query: 61  VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAA 120
           +AA YN+LQ+CKCWISA PK G SKLG+   I +FW SF LDQA AY++FAANTAG+EAA
Sbjct: 61  LAAAYNILQICKCWISAHPK-GTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAA 120

Query: 121 LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF 180
           LLA+TGA++ QWMKLCNRF RFC+Q GGAFLCGY A   M AI  IS+FNLF HYSP+ F
Sbjct: 121 LLAVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLF 180

Query: 181 LRPKS 186
            R KS
Sbjct: 181 FRLKS 183

BLAST of MS025316 vs. ExPASy TrEMBL
Match: A0A6J1HQ91 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465689 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 9.9e-66
Identity = 130/185 (70.27%), Postives = 152/185 (82.16%), Query Frame = 0

Query: 1   MGFFGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS 60
           MGF+ D +TEGILR  AIIGL+LTV LLGFDKQTTQIFH+DKKA+FRSL + +VIMYV+S
Sbjct: 1   MGFW-DQKTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDS 60

Query: 61  VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAA 120
           +AA YN+LQ+C CWISAQPK G SKLG+   I +FW SF LDQA AY++FAANTAG+EAA
Sbjct: 61  LAAAYNILQICNCWISAQPK-GTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAA 120

Query: 121 LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF 180
           LLA+TGA++ QWMKLCNRF RFC+Q GGAFLCGY A   M AI  IS+FNLF HY P+ F
Sbjct: 121 LLAVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYLPNLF 180

Query: 181 LRPKS 186
            R KS
Sbjct: 181 FRLKS 183

BLAST of MS025316 vs. ExPASy TrEMBL
Match: A0A6J1ET65 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111436264 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 3.0e-62
Identity = 123/181 (67.96%), Postives = 146/181 (80.66%), Query Frame = 0

Query: 4   FGDLRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAA 63
           FGD RTE ILR CAII L+LTV L+GFDK+TTQIF VDK A+FRSL +L VIM+V+S+AA
Sbjct: 3   FGDARTEWILRLCAIICLVLTVFLMGFDKETTQIFGVDKTASFRSLGALKVIMFVDSIAA 62

Query: 64  GYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLA 123
            YN+LQL KCWISA PK    +L  H+ + +FW+SFF+DQA AY+ FAANTAG+EAA +A
Sbjct: 63  AYNILQLAKCWISAHPK-PPFQLQTHFHLPLFWLSFFIDQAGAYLTFAANTAGMEAAFVA 122

Query: 124 ITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRP 183
           + G    QWMKLCNRF R+C+QA GAFLCGYAA LTM  ISF+SAFNLF HYSP+HFLR 
Sbjct: 123 VRGNQEFQWMKLCNRFHRYCYQAAGAFLCGYAAVLTMLLISFLSAFNLFRHYSPNHFLRL 182

Query: 184 K 185
           K
Sbjct: 183 K 182

BLAST of MS025316 vs. TAIR 10
Match: AT4G25830.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 152.5 bits (384), Expect = 3.3e-37
Identity = 80/177 (45.20%), Postives = 106/177 (59.89%), Query Frame = 0

Query: 9   TEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAAGYNLL 68
           TE ILR C +  L+LT CL+G D QT +I ++ K  +FR L +L   +Y++ V A YNL+
Sbjct: 7   TEVILRLCIVFFLLLTSCLIGLDSQTKEIAYIHKNVSFRYLLALEAELYIDVVVAAYNLV 66

Query: 69  QLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLAITGAY 128
           QL   W + + K    K          W S+ LDQ +AYV+FA  +A  + +LL +TG+ 
Sbjct: 67  QLGLGWYNVEQKTSNPK----------WFSYLLDQTAAYVVFAGTSAAAQHSLLVVTGSR 126

Query: 129 NLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRPKS 186
            LQWMK C +FTRFCFQ G A +  Y A+  M  +S ISAFNLF  YSP  F R KS
Sbjct: 127 ELQWMKWCYKFTRFCFQMGSAIILNYIAAALMVLLSSISAFNLFRLYSPKRFFRFKS 173

BLAST of MS025316 vs. TAIR 10
Match: AT2G35760.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 103.2 bits (256), Expect = 2.3e-22
Identity = 64/178 (35.96%), Postives = 93/178 (52.25%), Query Frame = 0

Query: 9   TEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAAGYNLL 68
           TE ILR    +  ++   L+  D Q  +IF + KKA F  +++L++++ VN +AAGY+L+
Sbjct: 30  TELILRCLVCVLALVAAILIATDVQVREIFMIQKKAKFTDMKALVLLVVVNGIAAGYSLV 89

Query: 69  QLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLAITGAY 128
           Q  +C +      G  K    +   + W  FF DQA AY+  A   A  ++A  A  G  
Sbjct: 90  QAVRCVV------GLMKGRVLFSKPLAWAIFFGDQAVAYLCVAGVAAAAQSAAFAKLGEP 149

Query: 129 NLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHFLRPKSK 187
            LQWMK+CN + +FC Q G        A + M  IS ISAF +F  Y  S   RP S+
Sbjct: 150 ELQWMKICNMYGKFCNQVGEGIASALFACIGMVLISCISAFGVFRLYGGSK-SRPSSR 200

BLAST of MS025316 vs. TAIR 10
Match: AT4G16442.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 83.2 bits (204), Expect = 2.4e-16
Identity = 53/167 (31.74%), Postives = 86/167 (51.50%), Query Frame = 0

Query: 9   TEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNSVAAGYNLL 68
           TE +LR    +  +L + L+  D +   IF + K A +  +++++ ++  N +AA Y+LL
Sbjct: 11  TELLLRCSISVFALLALILVVTDTEVKLIFTIKKTAKYTDMKAVVFLVVANGIAAVYSLL 70

Query: 69  QLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLAITGAY 128
           Q  +C +      G  K    +   + W  F  DQA AY+  AA  A  E+ ++A  G  
Sbjct: 71  QSVRCVV------GTMKGKVLFSKPLAWAFFSGDQAMAYLNVAAIAATAESGVIAREGEE 130

Query: 129 NLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHY 176
           +LQWM++C  + +FC Q          AS+ M  +S ISAF+LF  Y
Sbjct: 131 DLQWMRVCTMYGKFCNQMAIGVSSALLASIAMVFVSCISAFSLFRLY 171

BLAST of MS025316 vs. TAIR 10
Match: AT4G15610.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 50.1 bits (118), Expect = 2.3e-06
Identity = 41/169 (24.26%), Postives = 76/169 (44.97%), Query Frame = 0

Query: 9   TEGILRFCAIIGLILTVCLLGFDKQTTQIFHVD-----KKATFRSLRSLIVIMYVNSVAA 68
           T+ +LRF      + ++ ++   KQT  IF          A F +  +LI  +   SVA 
Sbjct: 29  TQVVLRFVLFAATLTSIVVMVTSKQTKNIFLPGTPIRIPAAEFTNSPALIYFVVALSVAC 88

Query: 69  GYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLA 128
            Y++       +S    + A K  +   + +  ++  +D     ++ +A  AG   A L 
Sbjct: 89  FYSI-------VSTFVTVSAFKKHSCSAVLLLNLA-IMDAVMVGIVASATGAGGGVAYLG 148

Query: 129 ITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLF 173
           + G   ++W K+C+ + +FC   GGA      AS+ +  +S IS  +L+
Sbjct: 149 LKGNKEVRWGKICHIYDKFCRHVGGAIAVSLFASVVLLLLSIISVLSLY 189

BLAST of MS025316 vs. TAIR 10
Match: AT4G20390.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 45.1 bits (105), Expect = 7.4e-05
Identity = 43/168 (25.60%), Postives = 73/168 (43.45%), Query Frame = 0

Query: 13  LRFCAIIGLILTVCLLGFDKQT-----TQIFHVDKKAT----FRSLRSLIVIMYVNSVAA 72
           LR  A +  +    ++  +K+T       I  V  KAT    F+   + +  +  N + +
Sbjct: 22  LRIFAFMATLAAAIVMSLNKETKTLVVATIGTVPIKATLTAKFQHTPAFVFFVIANVMVS 81

Query: 73  GYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQASAYVIFAANTAGVEAALLA 132
            +NLL +     S + +    +L     I I      LD  +A ++ AA  A V  A L 
Sbjct: 82  FHNLLMIVVQIFSRKLEYKGLRL---LSIAI------LDMLNATLVSAAANAAVFVAELG 141

Query: 133 ITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNL 172
             G  + +W K+C+RFT +C    GA +  +A  + M  +S +S   L
Sbjct: 142 KNGNKHAKWNKVCDRFTTYCDHGAGAIIAAFAGVILMLLVSAVSISRL 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153293.11.8e-9898.92CASP-like protein 2C1 [Momordica charantia][more]
XP_038889630.14.1e-7474.18CASP-like protein 2C1 [Benincasa hispida][more]
XP_004149074.12.0e-7374.18CASP-like protein 2C1 [Cucumis sativus] >KGN61092.1 hypothetical protein Csa_021... [more]
XP_023537147.14.9e-6771.89CASP-like protein 2C1 [Cucurbita pepo subsp. pepo] >KAG6586191.1 CASP-like prote... [more]
XP_022938059.11.8e-6671.35CASP-like protein 2C1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
B9IM092.7e-4453.11CASP-like protein 2C1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_578614 PE=3 S... [more]
A7Q6G62.3e-4354.19CASP-like protein 2C1 OS=Vitis vinifera OX=29760 GN=VIT_11s0052g01140 PE=2 SV=1[more]
Q5K4H91.3e-4148.02CASP-like protein XL3 OS=Gossypium hirsutum OX=3635 GN=XL3 PE=2 SV=1[more]
C6SZ043.4e-3950.00CASP-like protein 2C1 OS=Glycine max OX=3847 PE=2 SV=1[more]
Q8L8U94.6e-3645.20CASP-like protein 2C1 OS=Arabidopsis thaliana OX=3702 GN=At4g25830 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DH368.9e-9998.92CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111020824 PE=3 SV=1[more]
A0A0A0LK629.9e-7474.18CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G048830 PE=3 SV=1[more]
A0A6J1FCZ29.0e-6771.35CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444261 PE=3 SV=1[more]
A0A6J1HQ919.9e-6670.27CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465689 PE=3 SV=1[more]
A0A6J1ET653.0e-6267.96CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111436264 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G25830.13.3e-3745.20Uncharacterised protein family (UPF0497) [more]
AT2G35760.12.3e-2235.96Uncharacterised protein family (UPF0497) [more]
AT4G16442.12.4e-1631.74Uncharacterised protein family (UPF0497) [more]
AT4G15610.12.3e-0624.26Uncharacterised protein family (UPF0497) [more]
AT4G20390.17.4e-0525.60Uncharacterised protein family (UPF0497) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006459Casparian strip membrane proteinTIGRFAMTIGR01569TIGR01569coord: 12..170
e-value: 1.8E-22
score: 77.8
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 10..158
e-value: 3.7E-29
score: 101.5
NoneNo IPR availablePANTHERPTHR11615NITRATE, FORMATE, IRON DEHYDROGENASEcoord: 7..185
NoneNo IPR availablePANTHERPTHR11615:SF241CASP-LIKE PROTEIN 2C1coord: 7..185

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS025316.1MS025316.1mRNA