MS024898 (gene) Bitter gourd (TR) v1

Overview
NameMS024898
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionchitotriosidase-1-like
Locationscaffold83: 34076 .. 34810 (+)
RNA-Seq ExpressionMS024898
SyntenyMS024898
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTTTTTAGGCTGCAACCAGATAAGTAGTCTATGTATTTATAAACCTTTTTTAGGAATAATATCTTTTCAACTTGAAATTCTTAACATTAACTAAAAACTAAAACATATATTTAGTCTAGTTAAATAGTAAATTGCACCCACCATTCTTTCATTTGCTCTTTTTATACACTCTTAACATTGGTTACTTGTGTTAATTCAAGCCTATCTCAACGGGTCAAGATATGCATCTGTGTGTTCGATCTCCACATACATGTTGTTATACTAAAAAAAGCCTTTGATTGGTGATTCCTTGCATTGAATTTATGGAACAGGTATCGAGTCATGGATCAAATGTTATCCTAATTTGCCTTCCCAACAGATTGTATTTGGAATTCCTTTCCACGGTTGGGCATGGAAACTTGCCGATCGTCTCCAGCACGACGTGTTTTCGGAGGCGGATGGAGCAGCAATAGGACACGATATTTCTTCAGATGGCCAAATCTACTACTCCAATATTAAGAAGTTTATAGAGAGTAATAAGGCTAAGAATCTAAAAAATGACGGCAAATATGTAATTGGGTACACATATTTTGATTCAACTTGGATTGCCTACGAAAATGAAGAAACCATACCAGAGAAGATTAGATTGGCAAAGTCCCACCACGTGCTTGGTTACTTTGCTTCCAACATTGCAGCCGATGATGATGCTAACACTCTTGCTAACGCAGCTAGAACCAATAATTGGCCGTGA

mRNA sequence

ATGAGTTTTTTAGGCTGCAACCAGATAAGTAGTATCGAGTCATGGATCAAATGTTATCCTAATTTGCCTTCCCAACAGATTGTATTTGGAATTCCTTTCCACGGTTGGGCATGGAAACTTGCCGATCGTCTCCAGCACGACGTGTTTTCGGAGGCGGATGGAGCAGCAATAGGACACGATATTTCTTCAGATGGCCAAATCTACTACTCCAATATTAAGAAGTTTATAGAGAGTAATAAGGCTAAGAATCTAAAAAATGACGGCAAATATGTAATTGGGTACACATATTTTGATTCAACTTGGATTGCCTACGAAAATGAAGAAACCATACCAGAGAAGATTAGATTGGCAAAGTCCCACCACGTGCTTGGTTACTTTGCTTCCAACATTGCAGCCGATGATGATGCTAACACTCTTGCTAACGCAGCTAGAACCAATAATTGGCCGTGA

Coding sequence (CDS)

ATGAGTTTTTTAGGCTGCAACCAGATAAGTAGTATCGAGTCATGGATCAAATGTTATCCTAATTTGCCTTCCCAACAGATTGTATTTGGAATTCCTTTCCACGGTTGGGCATGGAAACTTGCCGATCGTCTCCAGCACGACGTGTTTTCGGAGGCGGATGGAGCAGCAATAGGACACGATATTTCTTCAGATGGCCAAATCTACTACTCCAATATTAAGAAGTTTATAGAGAGTAATAAGGCTAAGAATCTAAAAAATGACGGCAAATATGTAATTGGGTACACATATTTTGATTCAACTTGGATTGCCTACGAAAATGAAGAAACCATACCAGAGAAGATTAGATTGGCAAAGTCCCACCACGTGCTTGGTTACTTTGCTTCCAACATTGCAGCCGATGATGATGCTAACACTCTTGCTAACGCAGCTAGAACCAATAATTGGCCGTGA

Protein sequence

MSFLGCNQISSIESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIAADDDANTLANAARTNNWP
Homology
BLAST of MS024898 vs. NCBI nr
Match: XP_022143191.1 (chitotriosidase-1-like [Momordica charantia])

HSP 1 Score: 278.1 bits (710), Expect = 4.5e-71
Identity = 129/139 (92.81%), Postives = 134/139 (96.40%), Query Frame = 0

Query: 10  SSIESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY 69
           S IE+WIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY
Sbjct: 216 SGIEAWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY 275

Query: 70  SNIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASN 129
           SNIKKFIESNKAKNLKNDGKYVIGYTYFD+TWIAYENE TIPEK+RLAKS HVLGYFASN
Sbjct: 276 SNIKKFIESNKAKNLKNDGKYVIGYTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASN 335

Query: 130 IAADDDANTLANAARTNNW 149
           IAADDD NTLANAA+ N+W
Sbjct: 336 IAADDDVNTLANAAKANSW 354

BLAST of MS024898 vs. NCBI nr
Match: XP_022159350.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 172.9 bits (437), Expect = 2.0e-39
Identity = 85/136 (62.50%), Postives = 105/136 (77.21%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHD-ISSDGQIYYS 71
           I++WI+   NLP +QIVFGIPFHGWAWKLA+  +HDVFS+A G A G   IS +GQI  S
Sbjct: 223 IDAWIQSLANLPRKQIVFGIPFHGWAWKLANPDKHDVFSKALGPAKGDQYISQNGQIDXS 282

Query: 72  NIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNI 131
           N+K+FI+ N A+ +K+D +Y I Y Y  +TWIAYE+E TIP+KI  AKS  VLGYFA NI
Sbjct: 283 NVKRFIKQNHAQYVKDDSRYSISYAYAGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNI 342

Query: 132 AADDDANTLANAARTN 147
           AADD+ NTLANAA+TN
Sbjct: 343 AADDEVNTLANAAQTN 358

BLAST of MS024898 vs. NCBI nr
Match: XP_023545402.1 (acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 170.6 bits (431), Expect = 1.0e-38
Identity = 79/135 (58.52%), Postives = 102/135 (75.56%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSN 71
           I SW +   NLP ++IVFGIPFHGWAWKL +  QH +F++ADGAA G  I   GQI+YS+
Sbjct: 214 IRSWAQSLHNLPHKKIVFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSD 273

Query: 72  IKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIA 131
           ++KFIE N+A     D  Y++ YTYF++TWI YE++ TI +KI LAK+  VLGYFA NIA
Sbjct: 274 VQKFIEDNQAGVEDKDKSYLVAYTYFETTWITYESDYTIADKIALAKADKVLGYFAFNIA 333

Query: 132 ADDDANTLANAARTN 147
           ADD +NTLANAA++N
Sbjct: 334 ADDASNTLANAAKSN 348

BLAST of MS024898 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 170.6 bits (431), Expect = 1.0e-38
Identity = 79/135 (58.52%), Postives = 102/135 (75.56%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSN 71
           I SW +   NLP ++IVFGIPFHGWAWKL +  QH +F++ADGAA G  I   GQI+YS+
Sbjct: 229 IRSWAQSLHNLPHKKIVFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSD 288

Query: 72  IKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIA 131
           ++KFIE N+A     D  Y++ YTYF++TWI YE++ TI +KI LAK+  VLGYFA NIA
Sbjct: 289 VQKFIEDNQAGVEDKDKSYLVAYTYFETTWITYESDYTIADKIALAKADKVLGYFAFNIA 348

Query: 132 ADDDANTLANAARTN 147
           ADD +NTLANAA++N
Sbjct: 349 ADDASNTLANAAKSN 363

BLAST of MS024898 vs. NCBI nr
Match: KAG7030350.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 170.2 bits (430), Expect = 1.3e-38
Identity = 78/135 (57.78%), Postives = 102/135 (75.56%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSN 71
           I SW++   NLP ++IVFGIPFHGWAWKL +  QH +F++ADGAA G  I   GQI+YS+
Sbjct: 223 IRSWVQSLHNLPHKKIVFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSD 282

Query: 72  IKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIA 131
           ++KFIE N+A     D  Y++ Y Y+++TWI YE+E TI +KI LAK+  VLGYFA NIA
Sbjct: 283 VQKFIEDNQAGVEDKDKSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIA 342

Query: 132 ADDDANTLANAARTN 147
           ADD +NTLANAA++N
Sbjct: 343 ADDASNTLANAAKSN 357

BLAST of MS024898 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.7e-18
Identity = 51/131 (38.93%), Postives = 78/131 (59.54%), Query Frame = 0

Query: 14  SWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIK 73
           SWI+    LP+++ V G P++G+AW+L +   H  ++   GAA    IS DG I Y  I+
Sbjct: 241 SWIQA--GLPAKKAVLGFPYYGYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIR 300

Query: 74  KFIESNKAKNLKNDGKYVIG-YTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIAA 133
           KFI  N A  + N    V+G Y Y  + WI Y++ ++I  K+R AK   +LGYF+ ++ A
Sbjct: 301 KFIVDNGATTVYN--STVVGDYCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGA 360

Query: 134 DDDANTLANAA 144
           DD++  L+ AA
Sbjct: 361 DDNSG-LSRAA 362

BLAST of MS024898 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 2.9e-12
Identity = 43/124 (34.68%), Postives = 68/124 (54.84%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSN 71
           I SWI     +P++++V G+P +G AW+L D   H V ++A G A    + +DG + Y  
Sbjct: 268 IGSWIG--SGVPAEKLVMGLPLYGRAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDE 327

Query: 72  IKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIA 131
           I  F +   AK +  D   V  Y+Y  +TWI Y++  +I +K++ A+S  + GYF   I 
Sbjct: 328 ILVFNKDTGAK-VVYDEVAVSFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIG 384

Query: 132 ADDD 136
            D D
Sbjct: 388 KDKD 384

BLAST of MS024898 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.1e-11
Identity = 45/137 (32.85%), Postives = 72/137 (52.55%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSN 71
           + SWIK    +  +++V G+  +G AW+L D   + V +EA G A      +DG + Y+ 
Sbjct: 255 LHSWIK--SGVRPEKLVMGLALYGRAWELKDPNVNGVGAEAVGPA----TDTDGSMNYNE 314

Query: 72  IKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIA 131
           I KF + + A N+  D   +  Y+Y  +TWI Y++  +I  K+R AKS  + GYF   + 
Sbjct: 315 ILKFNKQSGA-NVVYDKVAISFYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALG 374

Query: 132 ADDDANTLANAARTNNW 149
            D D +    A  +N W
Sbjct: 375 KDKDWSISKQA--SNAW 382

BLAST of MS024898 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.5e-08
Identity = 35/118 (29.66%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 9   ISSIESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIY 68
           +S + SWI  +  +  +++V G+P +G +WKL D   H + + + G+  G     +G + 
Sbjct: 245 VSGLLSWI--HSGVVPEKLVMGMPLYGKSWKLRDPNVHGIGAPSVGSGPG----VNGLMA 304

Query: 69  YSNIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYF 127
           Y  +  F     AK ++ D      Y+Y  STWI Y+N  T+  K+  A++  + GYF
Sbjct: 305 YFQVLDFNRQKSAK-VEYDVDTASVYSYSGSTWIGYDNPFTVSIKVGFAQALKLRGYF 355

BLAST of MS024898 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 1.6e-07
Identity = 36/118 (30.51%), Postives = 60/118 (50.85%), Query Frame = 0

Query: 9   ISSIESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIY 68
           +S + SWI+    +  ++IV G+P +G +WKL D   H + +   G   G     DG + 
Sbjct: 251 VSGLFSWIR--GGVAPEKIVMGMPLYGKSWKLQDPNVHGIGAPNVGPGPG----VDGGMA 310

Query: 69  YSNIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYF 127
           Y  +  F +   AK +  D +    Y+Y  STWI Y++  T+  K+  A++  + GYF
Sbjct: 311 YFQVVDFNKQMGAK-VVYDKETGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYF 361

BLAST of MS024898 vs. ExPASy TrEMBL
Match: A0A6J1CPI6 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.2e-71
Identity = 129/139 (92.81%), Postives = 134/139 (96.40%), Query Frame = 0

Query: 10  SSIESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY 69
           S IE+WIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY
Sbjct: 216 SGIEAWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY 275

Query: 70  SNIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASN 129
           SNIKKFIESNKAKNLKNDGKYVIGYTYFD+TWIAYENE TIPEK+RLAKS HVLGYFASN
Sbjct: 276 SNIKKFIESNKAKNLKNDGKYVIGYTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASN 335

Query: 130 IAADDDANTLANAARTNNW 149
           IAADDD NTLANAA+ N+W
Sbjct: 336 IAADDDVNTLANAAKANSW 354

BLAST of MS024898 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 9.8e-40
Identity = 85/136 (62.50%), Postives = 105/136 (77.21%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHD-ISSDGQIYYS 71
           I++WI+   NLP +QIVFGIPFHGWAWKLA+  +HDVFS+A G A G   IS +GQI  S
Sbjct: 223 IDAWIQSLANLPRKQIVFGIPFHGWAWKLANPDKHDVFSKALGPAKGDQYISQNGQIDXS 282

Query: 72  NIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNI 131
           N+K+FI+ N A+ +K+D +Y I Y Y  +TWIAYE+E TIP+KI  AKS  VLGYFA NI
Sbjct: 283 NVKRFIKQNHAQYVKDDSRYSISYAYAGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNI 342

Query: 132 AADDDANTLANAARTN 147
           AADD+ NTLANAA+TN
Sbjct: 343 AADDEVNTLANAAQTN 358

BLAST of MS024898 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.8e-38
Identity = 78/135 (57.78%), Postives = 101/135 (74.81%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSN 71
           I SW +   NLP ++IVFGIPFHGWAWKL +  QH +F++ADGAA G  I   GQI+YS+
Sbjct: 223 IRSWAQSLHNLPHKKIVFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSD 282

Query: 72  IKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIA 131
           ++KFIE N+A     D  Y++ Y Y+++TWI YE+E TI +KI LAK+  VLGYFA NIA
Sbjct: 283 VQKFIEDNQAGVEDKDKSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIA 342

Query: 132 ADDDANTLANAARTN 147
           ADD +NTLANAA++N
Sbjct: 343 ADDASNTLANAAKSN 357

BLAST of MS024898 vs. ExPASy TrEMBL
Match: A0A6J1CMZ0 (oviduct-specific glycoprotein-like OS=Momordica charantia OX=3673 GN=LOC111012959 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 2.4e-38
Identity = 83/136 (61.03%), Postives = 105/136 (77.21%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHD-ISSDGQIYYS 71
           I++WI+   NLP +QIVF IPFHGWAWKLA+  ++DVFS+A G A G+  IS DGQI YS
Sbjct: 154 IDAWIQSLANLPRKQIVFEIPFHGWAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYS 213

Query: 72  NIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNI 131
           N++KFI+ N A+ +K+D +Y I Y Y  STWIAYE+E  IP+KI  AKS  VLGYFA NI
Sbjct: 214 NVRKFIKENHAEYVKDDSRYSISYAYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNI 273

Query: 132 AADDDANTLANAARTN 147
           AAD++ NTLA+AA+TN
Sbjct: 274 AADNEVNTLASAAQTN 289

BLAST of MS024898 vs. ExPASy TrEMBL
Match: A0A6J1G4H5 (chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 7.0e-38
Identity = 78/135 (57.78%), Postives = 100/135 (74.07%), Query Frame = 0

Query: 12  IESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSN 71
           I SW +   NLP ++IVFGIPFHGWAWKL +  QH  F++ADGAA G  I   GQI+YS+
Sbjct: 223 IRSWAQSLHNLPHKKIVFGIPFHGWAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSD 282

Query: 72  IKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIA 131
           ++KFIE N+A     D  Y++ Y Y+++TWI YE+E TI +KI LAK+  VLGYFA NIA
Sbjct: 283 VQKFIEDNQAGVEDKDKSYLVAYAYYETTWITYESEYTIADKIALAKADKVLGYFAFNIA 342

Query: 132 ADDDANTLANAARTN 147
           ADD +NTLANAA++N
Sbjct: 343 ADDASNTLANAAKSN 357

BLAST of MS024898 vs. TAIR 10
Match: AT4G19770.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 99.0 bits (245), Expect = 3.4e-21
Identity = 49/125 (39.20%), Postives = 72/125 (57.60%), Query Frame = 0

Query: 10  SSIESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY 69
           S ++ WI     LP+++ V G P++GWAW LAD   H  + +  G A    IS DG+I Y
Sbjct: 128 SGVKDWIDA--GLPAEKAVLGFPYYGWAWTLADPKNHGYYVDTTGPA----ISDDGEISY 187

Query: 70  SNIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASN 129
           S +K +I  NKA  + +D   +  Y Y  +TWI Y++EE+I  K+  AK   +LGYF+  
Sbjct: 188 SQLKTWIVDNKATTV-HDNIVIGDYCYAGTTWIGYDSEESIVTKVIYAKQKGLLGYFSWQ 245

Query: 130 IAADD 135
           +  DD
Sbjct: 248 VGGDD 245

BLAST of MS024898 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 93.2 bits (230), Expect = 1.9e-19
Identity = 51/131 (38.93%), Postives = 78/131 (59.54%), Query Frame = 0

Query: 14  SWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIK 73
           SWI+    LP+++ V G P++G+AW+L +   H  ++   GAA    IS DG I Y  I+
Sbjct: 241 SWIQA--GLPAKKAVLGFPYYGYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIR 300

Query: 74  KFIESNKAKNLKNDGKYVIG-YTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASNIAA 133
           KFI  N A  + N    V+G Y Y  + WI Y++ ++I  K+R AK   +LGYF+ ++ A
Sbjct: 301 KFIVDNGATTVYN--STVVGDYCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGA 360

Query: 134 DDDANTLANAA 144
           DD++  L+ AA
Sbjct: 361 DDNSG-LSRAA 362

BLAST of MS024898 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 92.8 bits (229), Expect = 2.5e-19
Identity = 46/127 (36.22%), Postives = 73/127 (57.48%), Query Frame = 0

Query: 10  SSIESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY 69
           + + +W +    LP+++ V G P +G+AW L D   H+ ++ + G A    IS DG I Y
Sbjct: 235 AGVRAWTQA--GLPAKKAVLGFPLYGYAWCLTDAKNHNYYANSSGPA----ISPDGSIGY 294

Query: 70  SNIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASN 129
             I++FI  NKA  + N    V  Y Y   TWI Y++ ++I  K++ AK   +LGYF+ +
Sbjct: 295 DQIRRFIVDNKATMVYN-SNLVQNYCYAKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWH 354

Query: 130 IAADDDA 137
           I ADD++
Sbjct: 355 IGADDNS 354

BLAST of MS024898 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 86.7 bits (213), Expect = 1.8e-17
Identity = 47/125 (37.60%), Postives = 70/125 (56.00%), Query Frame = 0

Query: 10  SSIESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY 69
           S +  W++    LP+++ V G  + GWAW L D   +   +  DGAA    ISSDG I Y
Sbjct: 226 SGLSKWLEA--KLPAKKAVLGFSYCGWAWTLEDAENNGYDAATDGAA----ISSDGSITY 285

Query: 70  SNIKKFIESNKAKNLKNDGKYVIG-YTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFAS 129
           + I+ +I  N A    +    VIG Y Y  +TWI Y++ ++I  K+R AK   +LGYF+ 
Sbjct: 286 AKIRNYIIDNGAATFHDPA--VIGFYCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSW 342

Query: 130 NIAAD 134
           ++ AD
Sbjct: 346 HVGAD 342

BLAST of MS024898 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 85.5 bits (210), Expect = 3.9e-17
Identity = 49/136 (36.03%), Postives = 77/136 (56.62%), Query Frame = 0

Query: 10  SSIESWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYY 69
           S +  W +    LP+++ V G P++GWAW LAD    DV +  D    G  IS DG+I Y
Sbjct: 215 SGVRDWTEA--GLPAKKAVLGFPYYGWAWTLAD---PDV-NGYDANTTGPAISDDGEISY 274

Query: 70  SNIKKFIESNKAKNLKNDGKYVIGYTYFDSTWIAYENEETIPEKIRLAKSHHVLGYFASN 129
             ++ +I  N A  + +D   V  Y Y  +TWI Y++E++I  K+  AK   +LGYF+ +
Sbjct: 275 RQLQTWIVDNGATKV-HDDMMVGDYCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWH 334

Query: 130 IAADDDANTLANAART 146
           +  DD++  L++A  T
Sbjct: 335 VGGDDNSE-LSSAGST 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143191.14.5e-7192.81chitotriosidase-1-like [Momordica charantia][more]
XP_022159350.12.0e-3962.50chitinase-3-like protein 2 [Momordica charantia][more]
XP_023545402.11.0e-3858.52acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo][more]
XP_023521369.11.0e-3858.52acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo][more]
KAG7030350.11.3e-3857.78Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
O818622.7e-1838.93Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A1B1J8Z22.9e-1234.68Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
A0A072VEP04.1e-1132.85Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
A0A072UR651.5e-0829.66Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
U5N4E31.6e-0730.51Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CPI62.2e-7192.81chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1[more]
A0A6J1DYG49.8e-4062.50chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S... [more]
A0A6J1G2P41.8e-3857.78acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE... [more]
A0A6J1CMZ02.4e-3861.03oviduct-specific glycoprotein-like OS=Momordica charantia OX=3673 GN=LOC11101295... [more]
A0A6J1G4H57.0e-3857.78chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 ... [more]
Match NameE-valueIdentityDescription
AT4G19770.13.4e-2139.20Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19810.11.9e-1938.93Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.12.5e-1936.22Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19750.11.8e-1737.60Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.13.9e-1736.03Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 11..133
e-value: 2.2E-12
score: 47.4
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 1..149
score: 11.686648
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 33..104
e-value: 1.0E-9
score: 40.9
IPR029070Chitinase insertion domain superfamilySUPERFAMILY54556Chitinase insertion domaincoord: 34..104
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 11..142
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 11..142
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 15..141

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS024898.1MS024898.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process