MS024316 (gene) Bitter gourd (TR) v1

Overview


Name	MS024316
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Protein kinase domain-containing protein
Location	scaffold30: 3136480 .. 3138757 (-)
RNA-Seq Expression	MS024316
Synteny	MS024316

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGTTTTGTGTCATCATCTGTTCAGCTCAAGCATTTTCAGCAGCCTCACTTCCCACCCAAAGGCAAAAACAATAAAACAGAAAACCCAAGAAGCAAAGACAAACACAGAGAAGGGGGAACCTGAATTGGATATTTTGTTGCAAGCTTCTTCCTTTATGTTCTGCAGGCCAACTGAAGGATGCCTCGCCGCATACTTGAAGGTAAATGTAAACTCCCCACTGATTCGGACAGTTGTTCGAACCAGATATGAAACTGAACATTAACTTTGGCCTTTGTCTTGGCAAGAAATTTTAAATTGTAGGAGCCTTCCCTCTTTCCAGTAATGTTCATAGTGATTCAAATTGCATTTTGAAGCCAGAAAAATTTATATAATCCTAGTAATGAACTTCTGTGTGCGACAATCTCATGGTGCTTTGCTTTTTCTGTTGTCCCAGGAACCTCTGATGATCGCTCTACATCATCTCTGCTACATTCTCCAGTGTTCTTCTTTTTCCTCGGGATTCTAGCTGTGCTGGTAATATTGTTGATTCTCGTATTGATCTTTAGGAAGTCTCTCAAACCGAATAAGGTGGTGAAGTTATTAAGACAAACTGGAACAGCTCAAGGTAATAGAGTTAGTGGCAAGATCAGTAGAAAATAATAATGTTTACAAAATTGCAAAAAGTAAATATTTTCATCTCACGTTGCAGCATCAGCAGATTTGTTGAGTGAGAACCTCCACTCAATAAGTTACTTTGATTTTCACACATTGAAAAAGGCAACAAAGAACTTTGATCCGGCAAATCTTCTTGGACAGGGAGGATTTGGACCCGTCTATCTGGTATCTGAGTTTCATTGGCTAGAATAATTTGATGCACAAGAATTAGTTCCTGCTACATTTGCATCGCCATATGAAAAGATTCTATATTTCTCTAACATATATATGTATGCTAATGCAGTTGATGGGTTTAAATGCAAAATATGATTTGAATGTATTTGACAGATAAAGGTCTACTGGAGTCCAAGCATGCAGAATTTATTATTATATAATTAATTCGTGAAAAAGATCAATCCCACATTACGTTACTTCAAAACTTAGCTTTGGACTAAAGAGAAACTTATCTTTATAGGGAACGCTAGAAGATGGAAAATTGGTTGCAATTAAGAAACTTTCACTTAACAAATCCCAGCAAGGAGAACCTGAATTTCTATCAGAGGTCAGACTGATAACAAGCATCCAACACAAGAACCTTGTTCGCCTGCTTGGTTGTTGCTCAGATGGGCCTCAGCGGCTACTCGTGTATGAATACATGGAGAACAGGAGTTTGGATCTCATAATATATGGTACTAGTTATCCTTCTCTATCTGATCTTCCCTACCCTTTTACCTTCCAATACATAATGTTGGATTATCCCACAACATAGGACAAAGCGACCAATTCCTCAACTGGAACACCAGATTGAAGATTATCAGAGGCATTGCTAAAGGGTTGCAGTATCTACACGAGGATTCCCACCTAAGAATTATTCACCGGGACATCAAAGCAAGCAACATTCTTCTCGATGACAAATTTCAACCGAAGATTGGTGATTTTGGGCTGGCCAGGTTTTTCCCTGATGACCAAGCTTACCTTAGCACTACATTTGCTGGAACTCTGTAAGCCTTCCATTTAACTTCACTTACTGTGAGGCACGTCTGATAAAACATTGGCTACAAAATTTGATGTAGCGTTCTGTTGTTATAGCATTGTGACATGCCCTCCATTTGTTCAGGGGCTATACAGCACCAGAATATGCAATTAGGGGGGAACTGTCAGAGAAGGCAGATGTTTACAGTTTTGGAGTTCTTGTGCTTGAAATCATCAGTGGTAGAAAAAACACAAATCTTTCTTTACCATCCGAAATGCAGTATCTTCCTGAATATGTATGCATAAAATCCCTCAGCTATTATCAAGTATTTATCAATTTCTAAGGCATTCAAATATGATTATTTAAGGATACTATACAGGCATGGAAACTATATGAGATGTCAGCAGTAATCGAACTTGTGGATCTAAAGATGAAGGATGGTGGATATGTGGAGAAGGATGTAGCACATGCGATTCAGGTTGCTTTGTTGTGCCTTCAGCCCCATGGCAACCTGAGACCTGCAATGTCTGAGATTGTTGCCATGCTAACATACAAATTTGAAATCATTCAAACACCTTCGAAACCAGCTTTCTTGAACAGAAGGCATAAAAGGAACAGAGATCTCTCGAGGAATAATTGTGCAGATACCACTCCTCCTCTACAGTGA

mRNA sequence

ATGTTGGTTTTGTGTCATCATCTGTTCAGCTCAAGCATTTTCAGCAGCCTCACTTCCCACCCAAAGGCAAAAACAATAAAACAGAAAACCCAAGAAGCAAAGACAAACACAGAGAAGGGGGAACCTGAATTGGATATTTTGTTGCAAGCTTCTTCCTTTATGTTCTGCAGGCCAACTGAAGGATGCCTCGCCGCATACTTGAAGAAAAATTTATATAATCCTAGTAATGAACTTCTGTGTGCGACAATCTCATGGTGCTTTGCTTTTTCTGTTGTCCCAGGAACCTCTGATGATCGCTCTACATCATCTCTGCTACATTCTCCAGTGTTCTTCTTTTTCCTCGGGATTCTAGCTGTGCTGGTAATATTGTTGATTCTCGTATTGATCTTTAGGAAGTCTCTCAAACCGAATAAGGTGGTGAAGTTATTAAGACAAACTGGAACAGCTCAAGCATCAGCAGATTTGTTGAGTGAGAACCTCCACTCAATAAGTTACTTTGATTTTCACACATTGAAAAAGGCAACAAAGAACTTTGATCCGGCAAATCTTCTTGGACAGGGAGGATTTGGACCCGTCTATCTGGGAACGCTAGAAGATGGAAAATTGGTTGCAATTAAGAAACTTTCACTTAACAAATCCCAGCAAGGAGAACCTGAATTTCTATCAGAGGTCAGACTGATAACAAGCATCCAACACAAGAACCTTGTTCGCCTGCTTGGTTGTTGCTCAGATGGGCCTCAGCGGCTACTCGTGGGGGAACTGTCAGAGAAGGCAGATGTTTACAGTTTTGGAGTTCTTGTGCTTGAAATCATCAGTGGTAGAAAAAACACAAATCTTTCTTTACCATCCGAAATGCAGTATCTTCCTGAATATGCATGGAAACTATATGAGATGTCAGCAGTAATCGAACTTGTGGATCTAAAGATGAAGGATGGTGGATATGTGGAGAAGGATGTAGCACATGCGATTCAGGTTGCTTTGTTGTGCCTTCAGCCCCATGGCAACCTGAGACCTGCAATGTCTGAGATTGTTGCCATGCTAACATACAAATTTGAAATCATTCAAACACCTTCGAAACCAGCTTTCTTGAACAGAAGGCATAAAAGGAACAGAGATCTCTCGAGGAATAATTGTGCAGATACCACTCCTCCTCTACAGTGA

Coding sequence (CDS)

ATGTTGGTTTTGTGTCATCATCTGTTCAGCTCAAGCATTTTCAGCAGCCTCACTTCCCACCCAAAGGCAAAAACAATAAAACAGAAAACCCAAGAAGCAAAGACAAACACAGAGAAGGGGGAACCTGAATTGGATATTTTGTTGCAAGCTTCTTCCTTTATGTTCTGCAGGCCAACTGAAGGATGCCTCGCCGCATACTTGAAGAAAAATTTATATAATCCTAGTAATGAACTTCTGTGTGCGACAATCTCATGGTGCTTTGCTTTTTCTGTTGTCCCAGGAACCTCTGATGATCGCTCTACATCATCTCTGCTACATTCTCCAGTGTTCTTCTTTTTCCTCGGGATTCTAGCTGTGCTGGTAATATTGTTGATTCTCGTATTGATCTTTAGGAAGTCTCTCAAACCGAATAAGGTGGTGAAGTTATTAAGACAAACTGGAACAGCTCAAGCATCAGCAGATTTGTTGAGTGAGAACCTCCACTCAATAAGTTACTTTGATTTTCACACATTGAAAAAGGCAACAAAGAACTTTGATCCGGCAAATCTTCTTGGACAGGGAGGATTTGGACCCGTCTATCTGGGAACGCTAGAAGATGGAAAATTGGTTGCAATTAAGAAACTTTCACTTAACAAATCCCAGCAAGGAGAACCTGAATTTCTATCAGAGGTCAGACTGATAACAAGCATCCAACACAAGAACCTTGTTCGCCTGCTTGGTTGTTGCTCAGATGGGCCTCAGCGGCTACTCGTGGGGGAACTGTCAGAGAAGGCAGATGTTTACAGTTTTGGAGTTCTTGTGCTTGAAATCATCAGTGGTAGAAAAAACACAAATCTTTCTTTACCATCCGAAATGCAGTATCTTCCTGAATATGCATGGAAACTATATGAGATGTCAGCAGTAATCGAACTTGTGGATCTAAAGATGAAGGATGGTGGATATGTGGAGAAGGATGTAGCACATGCGATTCAGGTTGCTTTGTTGTGCCTTCAGCCCCATGGCAACCTGAGACCTGCAATGTCTGAGATTGTTGCCATGCTAACATACAAATTTGAAATCATTCAAACACCTTCGAAACCAGCTTTCTTGAACAGAAGGCATAAAAGGAACAGAGATCTCTCGAGGAATAATTGTGCAGATACCACTCCTCCTCTACAGTGA

Protein sequence

MLVLCHHLFSSSIFSSLTSHPKAKTIKQKTQEAKTNTEKGEPELDILLQASSFMFCRPTEGCLAAYLKKNLYNPSNELLCATISWCFAFSVVPGTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGTAQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLVGELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYLPEYAWKLYEMSAVIELVDLKMKDGGYVEKDVAHAIQVALLCLQPHGNLRPAMSEIVAMLTYKFEIIQTPSKPAFLNRRHKRNRDLSRNNCADTTPPLQ

Homology

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BLAST of MS024316 vs. NCBI nr
Match: XP_022134929.1 (putative serine/threonine-protein kinase [Momordica charantia])

HSP 1 Score: 529.6 bits (1363), Expect = 2.2e-146
Identity = 292/398 (73.37%), Postives = 294/398 (73.87%), Query Frame = 0


Query: 91  VVPGTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGTAQ 150
           ++ GTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGTAQ
Sbjct: 5   ILEGTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGTAQ 64

Query: 151 ASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSL 210
           ASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSL
Sbjct: 65  ASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSL 124

Query: 211 NKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV------------------- 270
           NKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV                   
Sbjct: 125 NKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLVYEYMENRSLDLIIYGQSDQ 184

Query: 271 ------------------------------------------------------------ 330
                                                                       
Sbjct: 185 FLNWNTRLKIIRGIAKGLQYLHEDSHLRIIHRDIKASNILLDDKFQPKIGDFGLARFFPD 244

Query: 331 -----------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYL 387
                                  GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYL
Sbjct: 245 DQAYLSTTFAGTLGYTAPEYAIRGELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYL 304

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BLAST of MS024316 vs. NCBI nr
Match: XP_023530971.1 (putative serine/threonine-protein kinase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 459.9 bits (1182), Expect = 2.1e-125
Identity = 262/401 (65.34%), Postives = 276/401 (68.83%), Query Frame = 0


Query: 91  VVPGTSDD--RSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGT 150
           ++ GTS+   +S+SSLLHSPVFFFFLG+L VLVILLIL+LIFRK LKPN VVKLLR TGT
Sbjct: 5   ILEGTSEGQMKSSSSLLHSPVFFFFLGMLVVLVILLILILIFRKFLKPNTVVKLLRPTGT 64

Query: 151 AQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKL 210
            QASADLLSENLHSISYFDFHTLKKATKNF+P NLLG GGFGPVYLGTLEDG+LVAIK+L
Sbjct: 65  TQASADLLSENLHSISYFDFHTLKKATKNFNPTNLLGLGGFGPVYLGTLEDGRLVAIKRL 124

Query: 211 SLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV----------------- 270
           SLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV                 
Sbjct: 125 SLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLVYEYMENKSLDLIIYGVS 184

Query: 271 ------------------------------------------------------------ 330
                                                                       
Sbjct: 185 DQFLNWNTRLKIIRGIAKGLQYLHEDSHLRIIHRDIKASNILLDDRFQPKIGDFGLARFF 244

Query: 331 -------------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQ 387
                                    GELSEKADVYSFGVLVLEIISGRKNTNLSLP+EMQ
Sbjct: 245 PDDQAYLSTTFAGTLGYTAPEYAIRGELSEKADVYSFGVLVLEIISGRKNTNLSLPTEMQ 304

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BLAST of MS024316 vs. NCBI nr
Match: XP_038879107.1 (putative serine/threonine-protein kinase [Benincasa hispida])

HSP 1 Score: 459.1 bits (1180), Expect = 3.6e-125
Identity = 256/391 (65.47%), Postives = 273/391 (69.82%), Query Frame = 0


Query: 99  RSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGTAQASADLLSE 158
           +S+SSLLHSPVF FFLG+L V+VILLIL+LIFRKS+K NKVVKLLRQTGT QAS DLLSE
Sbjct: 2   KSSSSLLHSPVFCFFLGMLVVVVILLILILIFRKSIKQNKVVKLLRQTGTTQASTDLLSE 61

Query: 159 NLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSLNKSQQGEP 218
           NLHS+SYFDFHTLKKATKNF+P NLLGQGGFGPVYLGTLEDG++VAIKKLSLNKSQQGEP
Sbjct: 62  NLHSLSYFDFHTLKKATKNFNPKNLLGQGGFGPVYLGTLEDGRMVAIKKLSLNKSQQGEP 121

Query: 219 EFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV--------------------------- 278
           EFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV                           
Sbjct: 122 EFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLVYEYMENRSLDLIIYGGSDQILNWNTRL 181

Query: 279 ------------------------------------------------------------ 338
                                                                       
Sbjct: 182 KIIRGIAKGLQYLHEDSHLRIIHRDIKANNILLDDKFQPKIGDFGLARFFPDDQAYLSTT 241

Query: 339 ---------------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYLPEYAWKLY 387
                          GELSEKADVYSFGVLVLEIISGRKNTNLSLP+EMQYLPEYAWKLY
Sbjct: 242 FAGTLGYTAPEYAIRGELSEKADVYSFGVLVLEIISGRKNTNLSLPTEMQYLPEYAWKLY 301

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BLAST of MS024316 vs. NCBI nr
Match: XP_008453534.1 (PREDICTED: putative serine/threonine-protein kinase [Cucumis melo])

HSP 1 Score: 458.4 bits (1178), Expect = 6.2e-125
Identity = 259/401 (64.59%), Postives = 280/401 (69.83%), Query Frame = 0


Query: 91  VVPGTSDD--RSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGT 150
           ++ G+S D  +S+SSLL SPVF FFLG+LAVLVILLIL+LIFRKSLK NKVVKLL Q+GT
Sbjct: 5   ILQGSSKDQMKSSSSLLQSPVFCFFLGMLAVLVILLILILIFRKSLKQNKVVKLLTQSGT 64

Query: 151 AQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKL 210
            QAS DLLS+NLHS+SYFDFHTLKKATKNF+P NLLGQGGFGPVYLGTLEDG+LVAIKKL
Sbjct: 65  TQASTDLLSDNLHSLSYFDFHTLKKATKNFNPKNLLGQGGFGPVYLGTLEDGRLVAIKKL 124

Query: 211 SLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV----------------- 270
           SLNKSQQGE EFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV                 
Sbjct: 125 SLNKSQQGEAEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLVYEYMENRSLDLIIYGGS 184

Query: 271 ------------------------------------------------------------ 330
                                                                       
Sbjct: 185 DQILNWNTRLKIIRGIAKGLQYLHEDSHLRIIHRDIKASNILLDNKFQPKIGDFGLARFF 244

Query: 331 -------------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQ 387
                                    GELSEKADVYSFGVLVLEIISGRKNTNLSLP+EMQ
Sbjct: 245 PDDQAYLSTTFAGTLGYTAPEYAIRGELSEKADVYSFGVLVLEIISGRKNTNLSLPTEMQ 304

[Close]

BLAST of MS024316 vs. NCBI nr
Match: XP_011657132.1 (putative serine/threonine-protein kinase [Cucumis sativus] >XP_011657135.1 putative serine/threonine-protein kinase [Cucumis sativus] >KAE8653173.1 hypothetical protein Csa_020058 [Cucumis sativus])

HSP 1 Score: 457.6 bits (1176), Expect = 1.1e-124
Identity = 259/402 (64.43%), Postives = 277/402 (68.91%), Query Frame = 0


Query: 91  VVPGTSDD---RSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTG 150
           ++ G+S D   +STSSLL SPVF FFLG+LAVLVILLIL+LIFRKS K NKVVKLLRQ+G
Sbjct: 5   ILQGSSKDQMMKSTSSLLQSPVFCFFLGMLAVLVILLILLLIFRKSFKQNKVVKLLRQSG 64

Query: 151 TAQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKK 210
           T QAS DLLS+NLHS+SYFDFHTLKKATKNF+P NLLGQGGFGPVYLGTLEDG+LVAIKK
Sbjct: 65  TTQASTDLLSDNLHSLSYFDFHTLKKATKNFNPTNLLGQGGFGPVYLGTLEDGRLVAIKK 124

Query: 211 LSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV---------------- 270
           LSLNKSQQGE EFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV                
Sbjct: 125 LSLNKSQQGEAEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLVYEYMENRSLDLIIYGG 184

Query: 271 ------------------------------------------------------------ 330
                                                                       
Sbjct: 185 SEQILNWNTRLKIIRGIAKGLQYLHEDSHLRIIHRDIKASNILLDDKFQPKIGDFGLARF 244

Query: 331 --------------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEM 387
                                     GELSEKADVYSFGVLVLEIISGRKNTNLSLP+EM
Sbjct: 245 FPDDQAYLSTTFAGTLGYTAPEYAIRGELSEKADVYSFGVLVLEIISGRKNTNLSLPTEM 304

[Close]

BLAST of MS024316 vs. ExPASy Swiss-Prot
Match: C0LGH2 (Probable LRR receptor-like serine/threonine-protein kinase At1g56130 OS=Arabidopsis thaliana OX=3702 GN=At1g56130 PE=2 SV=2)

HSP 1 Score: 156.0 bits (393), Expect = 8.7e-37
Identity = 98/301 (32.56%), Postives = 141/301 (46.84%), Query Frame = 0


Query: 166 FDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSLNKSQQGEPEFLSEVR 225
           F +  LK AT++FDP+N LG+GGFGPVY G L DG++VA+K LS+  S+QG+ +F++E+ 
Sbjct: 682 FTYSELKSATQDFDPSNKLGEGGFGPVYKGNLNDGRVVAVKLLSVG-SRQGKGQFVAEIV 741

Query: 226 LITSIQHKNLVRLLGCCSDGPQRLLV---------------------------------- 285
            I+S+ H+NLV+L GCC +G  R+LV                                  
Sbjct: 742 AISSVLHRNLVKLYGCCFEGEHRMLVYEYLPNGSLDQALFGDKTLHLDWSTRYEICLGVA 801

Query: 286 ------------------------------------------------------------ 345
                                                                       
Sbjct: 802 RGLVYLHEEASVRIVHRDVKASNILLDSRLVPQISDFGLAKLYDDKKTHISTRVAGTIGY 861

Query: 346 --------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYLPEYAWKLYEMSAVIE 365
                   G L+EK DVY+FGV+ LE++SGR N++ +L  E +YL E+AW L+E S  IE
Sbjct: 862 LAPEYAMRGHLTEKTDVYAFGVVALELVSGRPNSDENLEEEKKYLLEWAWNLHEKSRDIE 921

[Close]

BLAST of MS024316 vs. ExPASy Swiss-Prot
Match: C0LGH3 (Probable LRR receptor-like serine/threonine-protein kinase At1g56140 OS=Arabidopsis thaliana OX=3702 GN=At1g56140 PE=2 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 3.1e-34
Identity = 121/441 (27.44%), Postives = 189/441 (42.86%), Query Frame = 0


Query: 25  TIKQKTQEAKTNTEKGEPELDILLQASSFMFCRPTEGCLAAYLKKNLYNPSNELLCATIS 84
           T++   +E K N  +   E+  L  A     C P +G          Y P    + AT  
Sbjct: 568 TVRAVQREYKANVSQNHLEIH-LFWAGKGTCCIPIQGA---------YGPLISAVGATPD 627

Query: 85  WCFAFSVVPGTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLR 144
           +       P +     T +++        +G+  + +I  +++ I RK  K        R
Sbjct: 628 FTPTVGNRPPSKGKSMTGTIVG-----VIVGVGLLSIISGVVIFIIRKRRK--------R 687

Query: 145 QTGTAQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVA 204
            T       ++LS ++   + F +  LK AT++FDP+N LG+GGFGPVY G L DG+ VA
Sbjct: 688 YTD----DEEILSMDVKPYT-FTYSELKSATQDFDPSNKLGEGGFGPVYKGKLNDGREVA 747

Query: 205 IKKLSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV------------- 264
           +K LS+  S+QG+ +F++E+  I+++QH+NLV+L GCC +G  RLLV             
Sbjct: 748 VKLLSVG-SRQGKGQFVAEIVAISAVQHRNLVKLYGCCYEGEHRLLVYEYLPNGSLDQAL 807

Query: 265 ------------------------------------------------------------ 324
                                                                       
Sbjct: 808 FGEKTLHLDWSTRYEICLGVARGLVYLHEEARLRIVHRDVKASNILLDSKLVPKVSDFGL 867

Query: 325 -----------------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLP 364
                                        G L+EK DVY+FGV+ LE++SGR N++ +L 
Sbjct: 868 AKLYDDKKTHISTRVAGTIGYLAPEYAMRGHLTEKTDVYAFGVVALELVSGRPNSDENLE 927

[Close]

BLAST of MS024316 vs. ExPASy Swiss-Prot
Match: C0LGG8 (Probable LRR receptor-like serine/threonine-protein kinase At1g53430 OS=Arabidopsis thaliana OX=3702 GN=At1g53430 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.4e-33
Identity = 97/323 (30.03%), Postives = 141/323 (43.65%), Query Frame = 0


Query: 139 VVKLLRQTGTAQASADLLSENLHSISY----FDFHTLKKATKNFDPANLLGQGGFGPVYL 198
           V+ +LR TG         +E L  +      F    +K+AT NFDP N +G+GGFGPVY 
Sbjct: 626 VLVILRLTGYLGGKEVDENEELRGLDLQTGSFTLKQIKRATNNFDPENKIGEGGFGPVYK 685

Query: 199 GTLEDGKLVAIKKLSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV--- 258
           G L DG  +A+K+LS +KS+QG  EF++E+ +I+++QH NLV+L GCC +G + LLV   
Sbjct: 686 GVLADGMTIAVKQLS-SKSKQGNREFVTEIGMISALQHPNLVKLYGCCIEGKELLLVYEY 745

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 746 LENNSLARALFGTEKQRLHLDWSTRNKICIGIAKGLAYLHEESRLKIVHRDIKATNVLLD 805

Query: 319 -----------------------------------------GELSEKADVYSFGVLVLEI 354
                                                    G L++KADVYSFGV+ LEI
Sbjct: 806 LSLNAKISDFGLAKLNDDENTHISTRIAGTIGYMAPEYAMRGYLTDKADVYSFGVVCLEI 865

[Close]

BLAST of MS024316 vs. ExPASy Swiss-Prot
Match: C0LGG9 (Probable LRR receptor-like serine/threonine-protein kinase At1g53440 OS=Arabidopsis thaliana OX=3702 GN=At1g53440 PE=2 SV=2)

HSP 1 Score: 143.3 bits (360), Expect = 5.8e-33
Identity = 97/323 (30.03%), Postives = 141/323 (43.65%), Query Frame = 0


Query: 139 VVKLLRQTGTAQASADLLSENLHSISY----FDFHTLKKATKNFDPANLLGQGGFGPVYL 198
           V+ +LR TG         +E L  +      F    +K+AT NFDP N +G+GGFGPVY 
Sbjct: 624 VLVILRLTGYLGGKEVDENEELRGLDLQTGSFTLKQIKRATNNFDPENKIGEGGFGPVYK 683

Query: 199 GTLEDGKLVAIKKLSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV--- 258
           G L DG  +A+K+LS +KS+QG  EF++E+ +I+++QH NLV+L GCC +G + LLV   
Sbjct: 684 GVLADGMTIAVKQLS-SKSKQGNREFVTEIGMISALQHPNLVKLYGCCIEGKELLLVYEY 743

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 744 LENNSLARALFGTEKQRLHLDWSTRNKVCIGIAKGLAYLHEESRLKIVHRDIKATNVLLD 803

Query: 319 -----------------------------------------GELSEKADVYSFGVLVLEI 354
                                                    G L++KADVYSFGV+ LEI
Sbjct: 804 LSLNAKISDFGLAKLDEEENTHISTRIAGTIGYMAPEYAMRGYLTDKADVYSFGVVCLEI 863

[Close]

BLAST of MS024316 vs. ExPASy Swiss-Prot
Match: Q9SXB3 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11280 OS=Arabidopsis thaliana OX=3702 GN=At1g11280 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.8e-32
Identity = 89/310 (28.71%), Postives = 142/310 (45.81%), Query Frame = 0


Query: 158 ENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSLNKSQQGE 217
           + +  +++F+ +T++ AT NF+ +N LGQGGFGPVY GTL D K +A+K+LS + S QG 
Sbjct: 485 QEISGLTFFEMNTIRAATNNFNVSNKLGQGGFGPVYKGTLSDKKDIAVKRLS-SSSGQGT 544

Query: 218 PEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV-------------------------- 277
            EF++E++LI+ +QH+NLVRLLGCC DG ++LL+                          
Sbjct: 545 EEFMNEIKLISKLQHRNLVRLLGCCIDGEEKLLIYEFLVNKSLDTFLFDLTLKLQIDWPK 604

Query: 278 ------------------------------------------------------------ 337
                                                                       
Sbjct: 605 RFNIIQGVSRGLLYLHRDSCMRVIHRDLKVSNILLDDKMNPKISDFGLARMFQGTQHQDN 664

Query: 338 ------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYLPEYAW 363
                             G  SEK+D+Y+FGVL+LEIISG+K ++     E + L  +AW
Sbjct: 665 TRKVVGTLGYMSPEYAWTGMFSEKSDIYAFGVLLLEIISGKKISSFCCGEEGKTLLGHAW 724

[Close]

BLAST of MS024316 vs. ExPASy TrEMBL
Match: A0A6J1C042 (putative serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC111007063 PE=4 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 1.1e-146
Identity = 292/398 (73.37%), Postives = 294/398 (73.87%), Query Frame = 0


Query: 91  VVPGTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGTAQ 150
           ++ GTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGTAQ
Sbjct: 5   ILEGTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGTAQ 64

Query: 151 ASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSL 210
           ASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSL
Sbjct: 65  ASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSL 124

Query: 211 NKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV------------------- 270
           NKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV                   
Sbjct: 125 NKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLVYEYMENRSLDLIIYGQSDQ 184

Query: 271 ------------------------------------------------------------ 330
                                                                       
Sbjct: 185 FLNWNTRLKIIRGIAKGLQYLHEDSHLRIIHRDIKASNILLDDKFQPKIGDFGLARFFPD 244

Query: 331 -----------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYL 387
                                  GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYL
Sbjct: 245 DQAYLSTTFAGTLGYTAPEYAIRGELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYL 304

[Close]

BLAST of MS024316 vs. ExPASy TrEMBL
Match: A0A1S3BX99 (putative serine/threonine-protein kinase OS=Cucumis melo OX=3656 GN=LOC103494217 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 3.0e-125
Identity = 259/401 (64.59%), Postives = 280/401 (69.83%), Query Frame = 0


Query: 91  VVPGTSDD--RSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGT 150
           ++ G+S D  +S+SSLL SPVF FFLG+LAVLVILLIL+LIFRKSLK NKVVKLL Q+GT
Sbjct: 5   ILQGSSKDQMKSSSSLLQSPVFCFFLGMLAVLVILLILILIFRKSLKQNKVVKLLTQSGT 64

Query: 151 AQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKL 210
            QAS DLLS+NLHS+SYFDFHTLKKATKNF+P NLLGQGGFGPVYLGTLEDG+LVAIKKL
Sbjct: 65  TQASTDLLSDNLHSLSYFDFHTLKKATKNFNPKNLLGQGGFGPVYLGTLEDGRLVAIKKL 124

Query: 211 SLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV----------------- 270
           SLNKSQQGE EFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV                 
Sbjct: 125 SLNKSQQGEAEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLVYEYMENRSLDLIIYGGS 184

Query: 271 ------------------------------------------------------------ 330
                                                                       
Sbjct: 185 DQILNWNTRLKIIRGIAKGLQYLHEDSHLRIIHRDIKASNILLDNKFQPKIGDFGLARFF 244

Query: 331 -------------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQ 387
                                    GELSEKADVYSFGVLVLEIISGRKNTNLSLP+EMQ
Sbjct: 245 PDDQAYLSTTFAGTLGYTAPEYAIRGELSEKADVYSFGVLVLEIISGRKNTNLSLPTEMQ 304

[Close]

BLAST of MS024316 vs. ExPASy TrEMBL
Match: A0A5A7USQ2 (Putative serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001990 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 5.1e-125
Identity = 259/403 (64.27%), Postives = 280/403 (69.48%), Query Frame = 0


Query: 91  VVPGTSDD--RSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQTGT 150
           ++ G+S D  +S+SSLL SPVF FFLG+LAVLVILLIL+LIFRKSLK NKVVKLL Q+GT
Sbjct: 11  ILQGSSKDQMKSSSSLLQSPVFCFFLGMLAVLVILLILILIFRKSLKQNKVVKLLTQSGT 70

Query: 151 AQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKL 210
            QAS DLLS+NLHS+SYFDFHTLKKATKNF+P NLLGQGGFGPVYLGTLEDG+LVAIKKL
Sbjct: 71  TQASTDLLSDNLHSLSYFDFHTLKKATKNFNPKNLLGQGGFGPVYLGTLEDGRLVAIKKL 130

Query: 211 SLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV----------------- 270
           SLNKSQQGE EFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV                 
Sbjct: 131 SLNKSQQGEAEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLVYEYMENRSLDLIIYGKS 190

Query: 271 ------------------------------------------------------------ 330
                                                                       
Sbjct: 191 QDDQILNWNTRLKIIRGIAKGLQYLHEDSHLRIIHRDIKASNILLDNKFQPKIGDFGLAR 250

Query: 331 ---------------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSE 387
                                      GELSEKADVYSFGVLVLEIISGRKNTNLSLP+E
Sbjct: 251 FFPDDQAYLSTTFAGTLGYTAPEYAIRGELSEKADVYSFGVLVLEIISGRKNTNLSLPTE 310

[Close]

BLAST of MS024316 vs. ExPASy TrEMBL
Match: A0A6J1KNU3 (putative receptor-like protein kinase At4g00960 OS=Cucurbita maxima OX=3661 GN=LOC111497330 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 8.8e-109
Identity = 230/359 (64.07%), Postives = 238/359 (66.30%), Query Frame = 0


Query: 131 RKSLKPNKVVKLLRQTGTAQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFG 190
           RK L PNK+VKLL  TGT QASADLLSENLHSISYFDFHTLKKATKNF+PANLLG GGFG
Sbjct: 220 RKFLTPNKLVKLL--TGTTQASADLLSENLHSISYFDFHTLKKATKNFNPANLLGLGGFG 279

Query: 191 PVYLGTLEDGKLVAIKKLSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLL 250
           PVYLGTLEDG+LVAIK+LSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLL
Sbjct: 280 PVYLGTLEDGRLVAIKRLSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLL 339

Query: 251 V----------------------------------------------------------- 310
           V                                                           
Sbjct: 340 VYEYMENKSLDLIIYGVSDQFLNWNTRLKIIRGIAKGLQYLHEDSHLRIIHRDIKASNIL 399

Query: 311 -------------------------------------------GELSEKADVYSFGVLVL 370
                                                      GELSEKADVYSFGV+VL
Sbjct: 400 LDDRFQPKIGDFGLARFFPDDQAYLSTTFAGTLGYTAPEYAIRGELSEKADVYSFGVIVL 459

Query: 371 EIISGRKNTNLSLPSEMQYLPEYAWKLYEMSAVIELVDLKMKDGGYVEKDVAHAIQVALL 387
           EIISGRKNTNLSLP+EMQYLPEYAWKLYE SA+IELVD KMKD GYVEK+V HAIQVALL
Sbjct: 460 EIISGRKNTNLSLPTEMQYLPEYAWKLYERSALIELVDPKMKDDGYVEKNVVHAIQVALL 519

[Close]

BLAST of MS024316 vs. ExPASy TrEMBL
Match: A0A540NBZ9 (Protein kinase domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_005872 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 2.1e-94
Identity = 188/297 (63.30%), Postives = 225/297 (75.76%), Query Frame = 0


Query: 102 SSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLRQT-GTAQASADLLSENL 161
           +S   SPV FFFLG L  L+ILL+L+ +F K +KP    KL+        AS D+ S  L
Sbjct: 397 ASKSQSPVLFFFLGGLVTLIILLVLLFVFWKKIKPEDTKKLVAVVRRRPAASTDVFSGML 456

Query: 162 HSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSLNKSQQGEPEF 221
            +ISYFDF TLKKATKNF P NLLG GGFGPVY G L DG L+A KKL L+KSQQGE EF
Sbjct: 457 RTISYFDFKTLKKATKNFHPGNLLGVGGFGPVYRGRLGDGTLIAAKKLCLDKSQQGESEF 516

Query: 222 LSEVRLITSIQHKNLVRLLGCCSDGPQRLLV----------GELSEKADVYSFGVLVLEI 281
           L+EV+LITS+QH+NLVRL+GCCSDGPQRLLV          GELSE+AD+YSFGVLVLEI
Sbjct: 517 LTEVKLITSVQHRNLVRLIGCCSDGPQRLLVGYTAPEYAIRGELSERADIYSFGVLVLEI 576

Query: 282 ISGRKNTNLSLPSEMQYLPEYAWKLYEMSAVIELVDLKMKDGGYVEKDVAHAIQVALLCL 341
           ISGRKNT+L+L S+MQYLPEYAWKL+E S VI+L+D KMK+ GYVE+DV  AIQVA  CL
Sbjct: 577 ISGRKNTDLTLSSDMQYLPEYAWKLFETSNVIDLIDPKMKEDGYVERDVLQAIQVAFFCL 636

Query: 342 QPHGNLRPAMSEIVAMLTYKFEIIQTPSKPAFLNRRHKRNRDLSRNNCADTTP-PLQ 387
           QPH NLRP MSE+V MLT K E++ TP KPAFL RR  +N+++S ++ ++  P PLQ
Sbjct: 637 QPHPNLRPPMSEVVVMLTCKVEMVGTPMKPAFLGRRRTKNQNISWDSISEAFPSPLQ 693

[Close]

BLAST of MS024316 vs. TAIR 10
Match: AT1G56130.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 156.0 bits (393), Expect = 6.2e-38
Identity = 98/301 (32.56%), Postives = 141/301 (46.84%), Query Frame = 0


Query: 166 FDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSLNKSQQGEPEFLSEVR 225
           F +  LK AT++FDP+N LG+GGFGPVY G L DG++VA+K LS+  S+QG+ +F++E+ 
Sbjct: 682 FTYSELKSATQDFDPSNKLGEGGFGPVYKGNLNDGRVVAVKLLSVG-SRQGKGQFVAEIV 741

Query: 226 LITSIQHKNLVRLLGCCSDGPQRLLV---------------------------------- 285
            I+S+ H+NLV+L GCC +G  R+LV                                  
Sbjct: 742 AISSVLHRNLVKLYGCCFEGEHRMLVYEYLPNGSLDQALFGDKTLHLDWSTRYEICLGVA 801

Query: 286 ------------------------------------------------------------ 345
                                                                       
Sbjct: 802 RGLVYLHEEASVRIVHRDVKASNILLDSRLVPQISDFGLAKLYDDKKTHISTRVAGTIGY 861

Query: 346 --------GELSEKADVYSFGVLVLEIISGRKNTNLSLPSEMQYLPEYAWKLYEMSAVIE 365
                   G L+EK DVY+FGV+ LE++SGR N++ +L  E +YL E+AW L+E S  IE
Sbjct: 862 LAPEYAMRGHLTEKTDVYAFGVVALELVSGRPNSDENLEEEKKYLLEWAWNLHEKSRDIE 921

[Close]

BLAST of MS024316 vs. TAIR 10
Match: AT1G56120.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 149.4 bits (376), Expect = 5.8e-36
Identity = 125/441 (28.34%), Postives = 190/441 (43.08%), Query Frame = 0


Query: 25  TIKQKTQEAKTNTEKGEPELDILLQASSFMFCRPTEGCLAAYLKKNLYNPSNELLCATIS 84
           +++   +E KTN  +   E+  L  A     C P +G          Y P    + AT  
Sbjct: 585 SVRAVQREYKTNVSENHLEVH-LFWAGKGTCCIPIQGA---------YGPLIAAVSATPD 644

Query: 85  WCFAFSVVPGTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLR 144
           +    +  P +     T +++   V    L I A +VIL+I     RK  KP        
Sbjct: 645 FTPTVANRPPSKGKSRTGTIVGVIVGVGLLSIFAGVVILVI-----RKRRKP-------- 704

Query: 145 QTGTAQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVA 204
                    ++LS ++   + F +  LK AT++FD +N LG+GGFG VY G L DG+ VA
Sbjct: 705 ----YTDDEEILSMDVKPYT-FTYSELKNATQDFDLSNKLGEGGFGAVYKGNLNDGREVA 764

Query: 205 IKKLSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV------------- 264
           +K+LS+  S+QG+ +F++E+  I+S+ H+NLV+L GCC +G  RLLV             
Sbjct: 765 VKQLSIG-SRQGKGQFVAEIIAISSVLHRNLVKLYGCCFEGDHRLLVYEYLPNGSLDQAL 824

Query: 265 ------------------------------------------------------------ 324
                                                                       
Sbjct: 825 FGDKSLHLDWSTRYEICLGVARGLVYLHEEASVRIIHRDVKASNILLDSELVPKVSDFGL 884

Query: 325 -----------------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLP 364
                                        G L+EK DVY+FGV+ LE++SGRKN++ +L 
Sbjct: 885 AKLYDDKKTHISTRVAGTIGYLAPEYAMRGHLTEKTDVYAFGVVALELVSGRKNSDENLE 944

[Close]

BLAST of MS024316 vs. TAIR 10
Match: AT1G56145.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 147.5 bits (371), Expect = 2.2e-35
Identity = 103/355 (29.01%), Postives = 165/355 (46.48%), Query Frame = 0


Query: 119 VLVILLILVLIFRKSLKPNKVVKLLRQTGTAQASADLLSENLHSISY-FDFHTLKKATKN 178
           +L IL+I +L+F            +R+     A  ++L+ +LH   Y F +  L+ AT++
Sbjct: 640 MLCILVIAILLF------------IRRKRKRAADEEVLN-SLHIRPYTFSYSELRTATQD 699

Query: 179 FDPANLLGQGGFGPVYLGTLEDGKLVAIKKLSLNKSQQGEPEFLSEVRLITSIQHKNLVR 238
           FDP+N LG+GGFGPV+ G L DG+ +A+K+LS+  S+QG+ +F++E+  I+++QH+NLV+
Sbjct: 700 FDPSNKLGEGGFGPVFKGKLNDGREIAVKQLSV-ASRQGKGQFVAEIATISAVQHRNLVK 759

Query: 239 LLGCCSDGPQRLLV---------------------------------------------- 298
           L GCC +G QR+LV                                              
Sbjct: 760 LYGCCIEGNQRMLVYEYLSNKSLDQALFEEKSLQLGWSQRFEICLGVAKGLAYMHEESNP 819

Query: 299 --------------------------------------------------------GELS 358
                                                                   G L+
Sbjct: 820 RIVHRDVKASNILLDSDLVPKLSDFGLAKLYDDKKTHISTRVAGTIGYLSPEYVMLGHLT 879

Query: 359 EKADVYSFGVLVLEIISGRKNTNLSLPSEMQYLPEYAWKLYEMSAVIELVDLKMKDGGYV 371
           EK DV++FG++ LEI+SGR N++  L  + QYL E+AW L++    +E+VD  + +  + 
Sbjct: 880 EKTDVFAFGIVALEIVSGRPNSSPELDDDKQYLLEWAWSLHQEQRDMEVVDPDLTE--FD 939

[Close]

BLAST of MS024316 vs. TAIR 10
Match: AT1G56140.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 147.5 bits (371), Expect = 2.2e-35
Identity = 121/441 (27.44%), Postives = 189/441 (42.86%), Query Frame = 0


Query: 25  TIKQKTQEAKTNTEKGEPELDILLQASSFMFCRPTEGCLAAYLKKNLYNPSNELLCATIS 84
           T++   +E K N  +   E+  L  A     C P +G          Y P    + AT  
Sbjct: 568 TVRAVQREYKANVSQNHLEIH-LFWAGKGTCCIPIQGA---------YGPLISAVGATPD 627

Query: 85  WCFAFSVVPGTSDDRSTSSLLHSPVFFFFLGILAVLVILLILVLIFRKSLKPNKVVKLLR 144
           +       P +     T +++        +G+  + +I  +++ I RK  K        R
Sbjct: 628 FTPTVGNRPPSKGKSMTGTIVG-----VIVGVGLLSIISGVVIFIIRKRRK--------R 687

Query: 145 QTGTAQASADLLSENLHSISYFDFHTLKKATKNFDPANLLGQGGFGPVYLGTLEDGKLVA 204
            T       ++LS ++   + F +  LK AT++FDP+N LG+GGFGPVY G L DG+ VA
Sbjct: 688 YTD----DEEILSMDVKPYT-FTYSELKSATQDFDPSNKLGEGGFGPVYKGKLNDGREVA 747

Query: 205 IKKLSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV------------- 264
           +K LS+  S+QG+ +F++E+  I+++QH+NLV+L GCC +G  RLLV             
Sbjct: 748 VKLLSVG-SRQGKGQFVAEIVAISAVQHRNLVKLYGCCYEGEHRLLVYEYLPNGSLDQAL 807

Query: 265 ------------------------------------------------------------ 324
                                                                       
Sbjct: 808 FGEKTLHLDWSTRYEICLGVARGLVYLHEEARLRIVHRDVKASNILLDSKLVPKVSDFGL 867

Query: 325 -----------------------------GELSEKADVYSFGVLVLEIISGRKNTNLSLP 364
                                        G L+EK DVY+FGV+ LE++SGR N++ +L 
Sbjct: 868 AKLYDDKKTHISTRVAGTIGYLAPEYAMRGHLTEKTDVYAFGVVALELVSGRPNSDENLE 927

[Close]

BLAST of MS024316 vs. TAIR 10
Match: AT1G53430.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 143.7 bits (361), Expect = 3.2e-34
Identity = 97/323 (30.03%), Postives = 141/323 (43.65%), Query Frame = 0


Query: 139 VVKLLRQTGTAQASADLLSENLHSISY----FDFHTLKKATKNFDPANLLGQGGFGPVYL 198
           V+ +LR TG         +E L  +      F    +K+AT NFDP N +G+GGFGPVY 
Sbjct: 618 VLVILRLTGYLGGKEVDENEELRGLDLQTGSFTLKQIKRATNNFDPENKIGEGGFGPVYK 677

Query: 199 GTLEDGKLVAIKKLSLNKSQQGEPEFLSEVRLITSIQHKNLVRLLGCCSDGPQRLLV--- 258
           G L DG  +A+K+LS +KS+QG  EF++E+ +I+++QH NLV+L GCC +G + LLV   
Sbjct: 678 GVLADGMTIAVKQLS-SKSKQGNREFVTEIGMISALQHPNLVKLYGCCIEGKELLLVYEY 737

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 738 LENNSLARALFGTEKQRLHLDWSTRNKICIGIAKGLAYLHEESRLKIVHRDIKATNVLLD 797

Query: 319 -----------------------------------------GELSEKADVYSFGVLVLEI 354
                                                    G L++KADVYSFGV+ LEI
Sbjct: 798 LSLNAKISDFGLAKLNDDENTHISTRIAGTIGYMAPEYAMRGYLTDKADVYSFGVVCLEI 857

The following BLAST results are available for this feature:

BLAST of MS024316 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134929.1	2.2e-146	73.37	putative serine/threonine-protein kinase [Momordica charantia]	[more]
XP_023530971.1	2.1e-125	65.34	putative serine/threonine-protein kinase [Cucurbita pepo subsp. pepo]	[more]
XP_038879107.1	3.6e-125	65.47	putative serine/threonine-protein kinase [Benincasa hispida]	[more]
XP_008453534.1	6.2e-125	64.59	PREDICTED: putative serine/threonine-protein kinase [Cucumis melo]	[more]
XP_011657132.1	1.1e-124	64.43	putative serine/threonine-protein kinase [Cucumis sativus] >XP_011657135.1 putat...	[more]

BLAST of MS024316 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0LGH2	8.7e-37	32.56	Probable LRR receptor-like serine/threonine-protein kinase At1g56130 OS=Arabidop...	[more]
C0LGH3	3.1e-34	27.44	Probable LRR receptor-like serine/threonine-protein kinase At1g56140 OS=Arabidop...	[more]
C0LGG8	4.4e-33	30.03	Probable LRR receptor-like serine/threonine-protein kinase At1g53430 OS=Arabidop...	[more]
C0LGG9	5.8e-33	30.03	Probable LRR receptor-like serine/threonine-protein kinase At1g53440 OS=Arabidop...	[more]
Q9SXB3	3.8e-32	28.71	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11280 OS=Arabi...	[more]

BLAST of MS024316 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C042	1.1e-146	73.37	putative serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A1S3BX99	3.0e-125	64.59	putative serine/threonine-protein kinase OS=Cucumis melo OX=3656 GN=LOC103494217...	[more]
A0A5A7USQ2	5.1e-125	64.27	Putative serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A6J1KNU3	8.8e-109	64.07	putative receptor-like protein kinase At4g00960 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A540NBZ9	2.1e-94	63.30	Protein kinase domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_005...	[more]

BLAST of MS024316 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56130.1	6.2e-38	32.56	Leucine-rich repeat transmembrane protein kinase	[more]
AT1G56120.1	5.8e-36	28.34	Leucine-rich repeat transmembrane protein kinase	[more]
AT1G56145.1	2.2e-35	29.01	Leucine-rich repeat transmembrane protein kinase	[more]
AT1G56140.1	2.2e-35	27.44	Leucine-rich repeat transmembrane protein kinase	[more]
AT1G53430.1	3.2e-34	30.03	Leucine-rich repeat transmembrane protein kinase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 149..249 e-value: 4.6E-33 score: 115.4
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 250..350 e-value: 4.5E-9 score: 37.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 366..386
None	No IPR available	PANTHER	PTHR27006	PROMASTIGOTE SURFACE ANTIGEN PROTEIN PSA	coord: 157..252 coord: 251..359
None	No IPR available	PANTHER	PTHR27006:SF21	RECEPTOR-LIKE KINASE	coord: 157..252 coord: 251..359
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	PK_Tyr_Ser-Thr	coord: 183..255 e-value: 9.3E-12 score: 44.8
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 178..386 score: 11.863386
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 168..359

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS024316.1	MS024316.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity