MS024145 (gene) Bitter gourd (TR) v1

Overview


Name	MS024145
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ubiquitin
Location	scaffold36: 174057 .. 174677 (-)
RNA-Seq Expression	MS024145
Synteny	MS024145

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAATCAATTGAATCGCCTCATGGCATATTCCTTAAACCCGGCCCAACAAACCTAATTCCCATTCCCATATATACAAATTTGCAATCCGATTTTGCCCTAATCTGCCAATCTCCCCTCGTCGCCTTGGTTACCCACGACTGCGACGATATGCAGATCTTCGTGAAAACGCTGACAGGGAAGACCATAACTCTCGAGGTCGAGAGTTCTGATACCATCGATAATGTGAAGGCCAAGATTCAGGACAAGGAGGGAATACCGCCGGACCAGCAGCGTCTCATTTTTGCTGGTAAGCAGCTGGAGGATGGTCGAACTCTTGCCGACTACAACATCCAGAAAGAGTCCACCCTACACCTTGTGCTAAGGCTCCGTGGAGGTGCTAAGAAGCGCAAGAAGAAGACCTACACCAAACCTAAGAAAATCAAGCACAAGAAAAAGAAGGTCAAGCTCGCCGTGCTTCAGTTTTACAAGATCGATGATTCGGGAAAGGTTCAGAGGCTCAGGAAAGAGTGCCCCAACGCTGAATGCGGAGCTGGTACTTTTATGGCCAATCATTTTGATCGTCATTACTGTGGCAAGTGTGGTTTGACTTACGTGTATCAGAAGCCCGGCGGCGATTAG

mRNA sequence

ATGCAATCAATTGAATCGCCTCATGGCATATTCCTTAAACCCGGCCCAACAAACCTAATTCCCATTCCCATATATACAAATTTGCAATCCGATTTTGCCCTAATCTGCCAATCTCCCCTCGTCGCCTTGGTTACCCACGACTGCGACGATATGCAGATCTTCGTGAAAACGCTGACAGGGAAGACCATAACTCTCGAGGTCGAGAGTTCTGATACCATCGATAATGTGAAGGCCAAGATTCAGGACAAGGAGGGAATACCGCCGGACCAGCAGCGTCTCATTTTTGCTGGTAAGCAGCTGGAGGATGGTCGAACTCTTGCCGACTACAACATCCAGAAAGAGTCCACCCTACACCTTGTGCTAAGGCTCCGTGGAGGTGCTAAGAAGCGCAAGAAGAAGACCTACACCAAACCTAAGAAAATCAAGCACAAGAAAAAGAAGGTCAAGCTCGCCGTGCTTCAGTTTTACAAGATCGATGATTCGGGAAAGGTTCAGAGGCTCAGGAAAGAGTGCCCCAACGCTGAATGCGGAGCTGGTACTTTTATGGCCAATCATTTTGATCGTCATTACTGTGGCAAGTGTGGTTTGACTTACGTGTATCAGAAGCCCGGCGGCGATTAG

Coding sequence (CDS)

ATGCAATCAATTGAATCGCCTCATGGCATATTCCTTAAACCCGGCCCAACAAACCTAATTCCCATTCCCATATATACAAATTTGCAATCCGATTTTGCCCTAATCTGCCAATCTCCCCTCGTCGCCTTGGTTACCCACGACTGCGACGATATGCAGATCTTCGTGAAAACGCTGACAGGGAAGACCATAACTCTCGAGGTCGAGAGTTCTGATACCATCGATAATGTGAAGGCCAAGATTCAGGACAAGGAGGGAATACCGCCGGACCAGCAGCGTCTCATTTTTGCTGGTAAGCAGCTGGAGGATGGTCGAACTCTTGCCGACTACAACATCCAGAAAGAGTCCACCCTACACCTTGTGCTAAGGCTCCGTGGAGGTGCTAAGAAGCGCAAGAAGAAGACCTACACCAAACCTAAGAAAATCAAGCACAAGAAAAAGAAGGTCAAGCTCGCCGTGCTTCAGTTTTACAAGATCGATGATTCGGGAAAGGTTCAGAGGCTCAGGAAAGAGTGCCCCAACGCTGAATGCGGAGCTGGTACTTTTATGGCCAATCATTTTGATCGTCATTACTGTGGCAAGTGTGGTTTGACTTACGTGTATCAGAAGCCCGGCGGCGATTAG

Protein sequence

MQSIESPHGIFLKPGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD

Homology

[Close]

BLAST of MS024145 vs. NCBI nr
Match: XP_022132058.1 (ubiquitin-40S ribosomal protein S27a-like [Momordica charantia])

HSP 1 Score: 415.6 bits (1067), Expect = 2.5e-112
Identity = 205/206 (99.51%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MQSIESPHGIFLKPGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTG 60
           MQSIESPHGIFLKPGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTG
Sbjct: 16  MQSIESPHGIFLKPGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTG 75

Query: 61  KTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLV 120
           KTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLV
Sbjct: 76  KTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLV 135

Query: 121 LRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGT 180
           LRLRGG+KKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGT
Sbjct: 136 LRLRGGSKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGT 195

Query: 181 FMANHFDRHYCGKCGLTYVYQKPGGD 207
           FMANHFDRHYCGKCGLTYVYQKPGGD
Sbjct: 196 FMANHFDRHYCGKCGLTYVYQKPGGD 221

[Close]

BLAST of MS024145 vs. NCBI nr
Match: KJB49764.1 (hypothetical protein B456_008G136200, partial [Gossypium raimondii])

HSP 1 Score: 314.3 bits (804), Expect = 7.7e-82
Identity = 164/201 (81.59%), Postives = 171/201 (85.07%), Query Frame = 0


Query: 6   SPHGIFLKPGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTGKTITL 65
           SP  I++KP       IP + NL          P ++L       MQIFVKTLTGKTITL
Sbjct: 22  SPSCIYIKPNRL----IPCFRNL--------AEPSISLCNQRHPKMQIFVKTLTGKTITL 81

Query: 66  EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRG 125
           EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRG
Sbjct: 82  EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRG 141

Query: 126 GAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGTFMANH 185
           GAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYK+D+SGKVQRLRKECPNAECGAGTFMANH
Sbjct: 142 GAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKVDESGKVQRLRKECPNAECGAGTFMANH 201

Query: 186 FDRHYCGKCGLTYVYQKPGGD 207
           FDRHYCGKCGLTYVYQK GGD
Sbjct: 202 FDRHYCGKCGLTYVYQKAGGD 210

[Close]

BLAST of MS024145 vs. NCBI nr
Match: XP_022961646.1 (ubiquitin-40S ribosomal protein S27a-like [Cucurbita moschata] >XP_023547140.1 ubiquitin-40S ribosomal protein S27a-like [Cucurbita pepo subsp. pepo] >KAG6598224.1 Ubiquitin-40S ribosomal protein S27a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 312.0 bits (798), Expect = 3.8e-81
Identity = 155/156 (99.36%), Postives = 156/156 (100.00%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYK+DDSGKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKVDDSGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD 207
           CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD
Sbjct: 121 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD 156

[Close]

BLAST of MS024145 vs. NCBI nr
Match: PNT10630.1 (hypothetical protein POPTR_012G114000 [Populus trichocarpa])

HSP 1 Score: 311.6 bits (797), Expect = 5.0e-81
Identity = 163/190 (85.79%), Postives = 168/190 (88.42%), Query Frame = 0


Query: 17  TNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTGKTITLEVESSDTIDNV 76
           +N +P P  T      AL+C SP  +        MQIFVKTLTGKTITLEVESSDTIDNV
Sbjct: 19  SNTLPYPAKTRCS---ALLCISP--SENPKKEAKMQIFVKTLTGKTITLEVESSDTIDNV 78

Query: 77  KAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKKTYT 136
           KAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKKTYT
Sbjct: 79  KAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKKTYT 138

Query: 137 KPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGL 196
           KPKKIKHKKKKVKLAVLQFYK+DDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGL
Sbjct: 139 KPKKIKHKKKKVKLAVLQFYKVDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGL 198

Query: 197 TYVYQKPGGD 207
           TYVYQK GGD
Sbjct: 199 TYVYQKAGGD 203

[Close]

BLAST of MS024145 vs. NCBI nr
Match: XP_022725555.1 (ubiquitin-40S ribosomal protein S27a-like [Durio zibethinus])

HSP 1 Score: 310.8 bits (795), Expect = 8.6e-81
Identity = 163/193 (84.46%), Postives = 167/193 (86.53%), Query Frame = 0


Query: 14  PGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTGKTITLEVESSDTI 73
           P P N IP     +L   F + C  P  +        MQIFVKTLTGKTITLEVESSDTI
Sbjct: 4   PNPNNKIPYKFPPHL-FHFPIHCSRPRHSKPA----IMQIFVKTLTGKTITLEVESSDTI 63

Query: 74  DNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKK 133
           DNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKK
Sbjct: 64  DNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKK 123

Query: 134 TYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGK 193
           TYTKPKKIKHKKKKVKLAVLQFYK+DDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGK
Sbjct: 124 TYTKPKKIKHKKKKVKLAVLQFYKVDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGK 183

Query: 194 CGLTYVYQKPGGD 207
           CGLTYVYQK GGD
Sbjct: 184 CGLTYVYQKAGGD 191

[Close]

BLAST of MS024145 vs. ExPASy Swiss-Prot
Match: P62980 (Ubiquitin-40S ribosomal protein S27a OS=Solanum lycopersicum OX=4081 GN=UBI3 PE=1 SV=2)

HSP 1 Score: 305.8 bits (782), Expect = 3.6e-82
Identity = 152/156 (97.44%), Postives = 154/156 (98.72%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYK+DD+GKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKVDDTGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD 207
           CPNAECGAGTFMANHFDRHYCGKCGLTYVY K GGD
Sbjct: 121 CPNAECGAGTFMANHFDRHYCGKCGLTYVYNKAGGD 156

[Close]

BLAST of MS024145 vs. ExPASy Swiss-Prot
Match: P62981 (Ubiquitin-40S ribosomal protein S27a OS=Solanum tuberosum OX=4113 GN=UBI3 PE=2 SV=2)

HSP 1 Score: 305.8 bits (782), Expect = 3.6e-82
Identity = 152/156 (97.44%), Postives = 154/156 (98.72%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYK+DD+GKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKVDDTGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD 207
           CPNAECGAGTFMANHFDRHYCGKCGLTYVY K GGD
Sbjct: 121 CPNAECGAGTFMANHFDRHYCGKCGLTYVYNKAGGD 156

[Close]

BLAST of MS024145 vs. ExPASy Swiss-Prot
Match: P47905 (Ubiquitin-40S ribosomal protein S27a OS=Lupinus albus OX=3870 PE=3 SV=2)

HSP 1 Score: 296.2 bits (757), Expect = 2.9e-79
Identity = 148/152 (97.37%), Postives = 149/152 (98.03%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHK KKVKLAVLQFYK+DDSGKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKHKKVKLAVLQFYKVDDSGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQK 203
           CPN ECGAGTFMANHFDRHYCGKCG TYVYQK
Sbjct: 121 CPNTECGAGTFMANHFDRHYCGKCGKTYVYQK 152

[Close]

BLAST of MS024145 vs. ExPASy Swiss-Prot
Match: P59233 (Ubiquitin-40S ribosomal protein S27a-3 OS=Arabidopsis thaliana OX=3702 GN=RPS27AC PE=2 SV=2)

HSP 1 Score: 295.4 bits (755), Expect = 4.9e-79
Identity = 149/154 (96.75%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHK KKVKLAVLQFYKID SGKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKHKKVKLAVLQFYKIDGSGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPG 205
           CPNA CGAGTFMA+HFDRHYCGKCGLTYVYQK G
Sbjct: 121 CPNATCGAGTFMASHFDRHYCGKCGLTYVYQKEG 154

[Close]

BLAST of MS024145 vs. ExPASy Swiss-Prot
Match: P59232 (Ubiquitin-40S ribosomal protein S27a-2 OS=Arabidopsis thaliana OX=3702 GN=RPS27AB PE=2 SV=2)

HSP 1 Score: 295.0 bits (754), Expect = 6.4e-79
Identity = 148/154 (96.10%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHK KKVKLAVLQFYK+D SGKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKHKKVKLAVLQFYKVDGSGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPG 205
           CPNA CGAGTFMA+HFDRHYCGKCGLTYVYQK G
Sbjct: 121 CPNATCGAGTFMASHFDRHYCGKCGLTYVYQKEG 154

[Close]

BLAST of MS024145 vs. ExPASy TrEMBL
Match: A0A6J1BV75 (Ubiquitin OS=Momordica charantia OX=3673 GN=LOC111005021 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.2e-112
Identity = 205/206 (99.51%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MQSIESPHGIFLKPGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTG 60
           MQSIESPHGIFLKPGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTG
Sbjct: 16  MQSIESPHGIFLKPGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTG 75

Query: 61  KTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLV 120
           KTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLV
Sbjct: 76  KTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLV 135

Query: 121 LRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGT 180
           LRLRGG+KKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGT
Sbjct: 136 LRLRGGSKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGT 195

Query: 181 FMANHFDRHYCGKCGLTYVYQKPGGD 207
           FMANHFDRHYCGKCGLTYVYQKPGGD
Sbjct: 196 FMANHFDRHYCGKCGLTYVYQKPGGD 221

[Close]

BLAST of MS024145 vs. ExPASy TrEMBL
Match: A0A0D2T035 (Ubiquitin (Fragment) OS=Gossypium raimondii OX=29730 GN=B456_008G136200 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 3.8e-82
Identity = 164/201 (81.59%), Postives = 171/201 (85.07%), Query Frame = 0


Query: 6   SPHGIFLKPGPTNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTGKTITL 65
           SP  I++KP       IP + NL          P ++L       MQIFVKTLTGKTITL
Sbjct: 22  SPSCIYIKPNRL----IPCFRNL--------AEPSISLCNQRHPKMQIFVKTLTGKTITL 81

Query: 66  EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRG 125
           EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRG
Sbjct: 82  EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRG 141

Query: 126 GAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGTFMANH 185
           GAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYK+D+SGKVQRLRKECPNAECGAGTFMANH
Sbjct: 142 GAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKVDESGKVQRLRKECPNAECGAGTFMANH 201

Query: 186 FDRHYCGKCGLTYVYQKPGGD 207
           FDRHYCGKCGLTYVYQK GGD
Sbjct: 202 FDRHYCGKCGLTYVYQKAGGD 210

[Close]

BLAST of MS024145 vs. ExPASy TrEMBL
Match: A0A6J1HAY0 (Ubiquitin OS=Cucurbita moschata OX=3662 GN=LOC111462355 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.9e-81
Identity = 155/156 (99.36%), Postives = 156/156 (100.00%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYK+DDSGKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKVDDSGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD 207
           CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD
Sbjct: 121 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD 156

[Close]

BLAST of MS024145 vs. ExPASy TrEMBL
Match: A0A2K1YC75 (Ubiquitin OS=Populus trichocarpa OX=3694 GN=POPTR_012G114000 PE=3 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 2.4e-81
Identity = 163/190 (85.79%), Postives = 168/190 (88.42%), Query Frame = 0


Query: 17  TNLIPIPIYTNLQSDFALICQSPLVALVTHDCDDMQIFVKTLTGKTITLEVESSDTIDNV 76
           +N +P P  T      AL+C SP  +        MQIFVKTLTGKTITLEVESSDTIDNV
Sbjct: 19  SNTLPYPAKTRCS---ALLCISP--SENPKKEAKMQIFVKTLTGKTITLEVESSDTIDNV 78

Query: 77  KAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKKTYT 136
           KAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKKTYT
Sbjct: 79  KAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKKTYT 138

Query: 137 KPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGL 196
           KPKKIKHKKKKVKLAVLQFYK+DDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGL
Sbjct: 139 KPKKIKHKKKKVKLAVLQFYKVDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGL 198

Query: 197 TYVYQKPGGD 207
           TYVYQK GGD
Sbjct: 199 TYVYQKAGGD 203

[Close]

BLAST of MS024145 vs. ExPASy TrEMBL
Match: A0A0A0LQL6 (Ubiquitin OS=Cucumis sativus OX=3659 GN=Csa_2G348875 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 4.1e-81
Identity = 154/156 (98.72%), Postives = 156/156 (100.00%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKL+VLQFYK+DDSGKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLSVLQFYKVDDSGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD 207
           CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD
Sbjct: 121 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPGGD 156

[Close]

BLAST of MS024145 vs. TAIR 10
Match: AT3G62250.1 (ubiquitin 5 )

HSP 1 Score: 295.4 bits (755), Expect = 3.5e-80
Identity = 149/154 (96.75%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHK KKVKLAVLQFYKID SGKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKHKKVKLAVLQFYKIDGSGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPG 205
           CPNA CGAGTFMA+HFDRHYCGKCGLTYVYQK G
Sbjct: 121 CPNATCGAGTFMASHFDRHYCGKCGLTYVYQKEG 154

[Close]

BLAST of MS024145 vs. TAIR 10
Match: AT2G47110.1 (ubiquitin 6 )

HSP 1 Score: 295.0 bits (754), Expect = 4.5e-80
Identity = 148/154 (96.10%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHK KKVKLAVLQFYK+D SGKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKHKKVKLAVLQFYKVDGSGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPG 205
           CPNA CGAGTFMA+HFDRHYCGKCGLTYVYQK G
Sbjct: 121 CPNATCGAGTFMASHFDRHYCGKCGLTYVYQKEG 154

[Close]

BLAST of MS024145 vs. TAIR 10
Match: AT2G47110.2 (ubiquitin 6 )

HSP 1 Score: 295.0 bits (754), Expect = 4.5e-80
Identity = 148/154 (96.10%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHK KKVKLAVLQFYK+D SGKVQRLRKE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKHKKVKLAVLQFYKVDGSGKVQRLRKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQKPG 205
           CPNA CGAGTFMA+HFDRHYCGKCGLTYVYQK G
Sbjct: 121 CPNATCGAGTFMASHFDRHYCGKCGLTYVYQKEG 154

[Close]

BLAST of MS024145 vs. TAIR 10
Match: AT1G23410.1 (Ribosomal protein S27a / Ubiquitin family protein )

HSP 1 Score: 279.6 bits (714), Expect = 2.0e-75
Identity = 139/152 (91.45%), Postives = 145/152 (95.39%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 111 IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKIDDSGKVQRLRKE 170
           IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKH  KKVKLAVLQFYK+D SGKVQRL+KE
Sbjct: 61  IQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHTHKKVKLAVLQFYKVDGSGKVQRLKKE 120

Query: 171 CPNAECGAGTFMANHFDRHYCGKCGLTYVYQK 203
           CP+  CG GTFMA+HFDRHYCGKCG TYV++K
Sbjct: 121 CPSVSCGPGTFMASHFDRHYCGKCGTTYVFKK 152

[Close]

BLAST of MS024145 vs. TAIR 10
Match: AT5G03240.1 (polyubiquitin 3 )

HSP 1 Score: 150.2 bits (378), Expect = 1.8e-36
Identity = 76/77 (98.70%), Postives = 77/77 (100.00%), Query Frame = 0


Query: 51  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 110
           MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 288

Query: 111 IQKESTLHLVLRLRGGA 128
           IQKESTLHLVLRLRGG+
Sbjct: 289 IQKESTLHLVLRLRGGS 305

The following BLAST results are available for this feature:

BLAST of MS024145 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132058.1	2.5e-112	99.51	ubiquitin-40S ribosomal protein S27a-like [Momordica charantia]	[more]
KJB49764.1	7.7e-82	81.59	hypothetical protein B456_008G136200, partial [Gossypium raimondii]	[more]
XP_022961646.1	3.8e-81	99.36	ubiquitin-40S ribosomal protein S27a-like [Cucurbita moschata] >XP_023547140.1 u...	[more]
PNT10630.1	5.0e-81	85.79	hypothetical protein POPTR_012G114000 [Populus trichocarpa]	[more]
XP_022725555.1	8.6e-81	84.46	ubiquitin-40S ribosomal protein S27a-like [Durio zibethinus]	[more]

BLAST of MS024145 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P62980	3.6e-82	97.44	Ubiquitin-40S ribosomal protein S27a OS=Solanum lycopersicum OX=4081 GN=UBI3 PE=...	[more]
P62981	3.6e-82	97.44	Ubiquitin-40S ribosomal protein S27a OS=Solanum tuberosum OX=4113 GN=UBI3 PE=2 S...	[more]
P47905	2.9e-79	97.37	Ubiquitin-40S ribosomal protein S27a OS=Lupinus albus OX=3870 PE=3 SV=2	[more]
P59233	4.9e-79	96.75	Ubiquitin-40S ribosomal protein S27a-3 OS=Arabidopsis thaliana OX=3702 GN=RPS27A...	[more]
P59232	6.4e-79	96.10	Ubiquitin-40S ribosomal protein S27a-2 OS=Arabidopsis thaliana OX=3702 GN=RPS27A...	[more]

BLAST of MS024145 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BV75	1.2e-112	99.51	Ubiquitin OS=Momordica charantia OX=3673 GN=LOC111005021 PE=3 SV=1	[more]
A0A0D2T035	3.8e-82	81.59	Ubiquitin (Fragment) OS=Gossypium raimondii OX=29730 GN=B456_008G136200 PE=3 SV=...	[more]
A0A6J1HAY0	1.9e-81	99.36	Ubiquitin OS=Cucurbita moschata OX=3662 GN=LOC111462355 PE=3 SV=1	[more]
A0A2K1YC75	2.4e-81	85.79	Ubiquitin OS=Populus trichocarpa OX=3694 GN=POPTR_012G114000 PE=3 SV=1	[more]
A0A0A0LQL6	4.1e-81	98.72	Ubiquitin OS=Cucumis sativus OX=3659 GN=Csa_2G348875 PE=3 SV=1	[more]

BLAST of MS024145 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62250.1	3.5e-80	96.75	ubiquitin 5	[more]
AT2G47110.1	4.5e-80	96.10	ubiquitin 6	[more]
AT2G47110.2	4.5e-80	96.10	ubiquitin 6	[more]
AT1G23410.1	2.0e-75	91.45	Ribosomal protein S27a / Ubiquitin family protein	[more]
AT5G03240.1	1.8e-36	98.70	polyubiquitin 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019956	Ubiquitin domain	PRINTS	PR00348	UBIQUITIN	coord: 61..81 score: 85.2 coord: 82..102 score: 95.05 coord: 103..124 score: 91.18
IPR002906	Ribosomal protein S27a	SMART	SM01402	Ribosomal_S27_2	coord: 152..197 e-value: 5.6E-28 score: 108.9
IPR002906	Ribosomal protein S27a	PFAM	PF01599	Ribosomal_S27	coord: 152..197 e-value: 5.7E-26 score: 90.2
IPR000626	Ubiquitin-like domain	SMART	SM00213	ubq_7	coord: 51..122 e-value: 3.0E-35 score: 133.1
IPR000626	Ubiquitin-like domain	PFAM	PF00240	ubiquitin	coord: 53..124 e-value: 1.1E-33 score: 114.8
IPR000626	Ubiquitin-like domain	PROSITE	PS50053	UBIQUITIN_2	coord: 51..126 score: 30.452106
IPR038582	S27a-like superfamily	GENE3D	6.20.50.150		coord: 131..205 e-value: 6.8E-40 score: 137.3
None	No IPR available	GENE3D	3.10.20.90		coord: 51..130 e-value: 1.1E-45 score: 155.4
None	No IPR available	PANTHER	PTHR10666:SF363	UBIQUITIN-40S RIBOSOMAL PROTEIN S27A-1	coord: 45..196
None	No IPR available	PANTHER	PTHR10666	UBIQUITIN	coord: 45..196
None	No IPR available	CDD	cd01803	Ubl_ubiquitin	coord: 51..126 e-value: 4.81118E-55 score: 168.006
IPR019954	Ubiquitin conserved site	PROSITE	PS00299	UBIQUITIN_1	coord: 77..102
IPR011332	Zinc-binding ribosomal protein	SUPERFAMILY	57829	Zn-binding ribosomal proteins	coord: 150..202
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 34..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS024145.1	MS024145.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005840	ribosome
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003735	structural constituent of ribosome