MS023731 (gene) Bitter gourd (TR) v1

Overview


Name	MS023731
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	6-phosphogluconate dehydrogenase family protein
Location	scaffold570: 600411 .. 601346 (-)
RNA-Seq Expression	MS023731
Synteny	MS023731

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACTTCCGGCGACATCCTCAGCCCCTCAACCACCCGCGTCGGCTGGATCGGCACCGGCGTCATGGGCTGCTCCATGTGCTCCCATCTCATCAGAGCCGGCTTCAAACTCACCGTCTTCAACCGCACAATTTCCAAAGCCCAGCCCCTCCTCGATCTGGGCGCCGATTTGGCCTCCTCCCCACTCGCCGTCGCCGCCCAATCCGACGTCGTCTTCTCCATCGTCGGCTACCCCTCCGACGTCCGCTCCGTTCTCCTCGACCCCACCTCCGGCGCCCTCGCCGGCCTCCGCCCCGGCGGCATCCTCGTCGACATGACCACTTCCGAGCCTTCCCTCGCATCCGAAATCGCCGCCGCCGCCGCCGCCAAGGGCTGCGGGGCGATCGACGCTCCAGTCTCCGGCGGCGATCGCGGCGCCAAGAACGCCACGCTGGCAATCTTCGCCGGCGGCGACGAGGGTCAAGTCCGGCGGCTGAATCCGTTGTTTGCCCTGTTGGGGAAGGTGAATTACATGGGAGAGAGCGGGAAAGGGCAGTTCGCGAAGCTGGCGAATCAGATCACCATAGCTTCGACGATGGTGGGGCTGGTGGAGGGGATGATTTATGCGCACAAAGCGGGTCTGGATGTGGGTTTGTTCTTGAACGCCATTTCAACGGGCGCTGCGGGGTCGAAATCGCTGGATCTGTACGGGAGCAGGATATTGAAGAGGGATTTCGAAGCTGGGTTCTTCGTAAACCACTTTGTGAAGGATCTGGGAATCTGCCTGAAGGAGTGTCAGAATATGGGGATTGCTCTTCCGGGATTGGCATTGGCTCAGCAGCTTTATCTGTCGCTTAAGGCTCATGGGGAAGGGAATTTGGGCACTCAAGCGCTCATCTTATCCCTGGAGCGCCTCAACGACGTCTCGCTTCAATCTTCCACCGCCGCATCTTCT

mRNA sequence

ATGGCCACTTCCGGCGACATCCTCAGCCCCTCAACCACCCGCGTCGGCTGGATCGGCACCGGCGTCATGGGCTGCTCCATGTGCTCCCATCTCATCAGAGCCGGCTTCAAACTCACCGTCTTCAACCGCACAATTTCCAAAGCCCAGCCCCTCCTCGATCTGGGCGCCGATTTGGCCTCCTCCCCACTCGCCGTCGCCGCCCAATCCGACGTCGTCTTCTCCATCGTCGGCTACCCCTCCGACGTCCGCTCCGTTCTCCTCGACCCCACCTCCGGCGCCCTCGCCGGCCTCCGCCCCGGCGGCATCCTCGTCGACATGACCACTTCCGAGCCTTCCCTCGCATCCGAAATCGCCGCCGCCGCCGCCGCCAAGGGCTGCGGGGCGATCGACGCTCCAGTCTCCGGCGGCGATCGCGGCGCCAAGAACGCCACGCTGGCAATCTTCGCCGGCGGCGACGAGGGTCAAGTCCGGCGGCTGAATCCGTTGTTTGCCCTGTTGGGGAAGGTGAATTACATGGGAGAGAGCGGGAAAGGGCAGTTCGCGAAGCTGGCGAATCAGATCACCATAGCTTCGACGATGGTGGGGCTGGTGGAGGGGATGATTTATGCGCACAAAGCGGGTCTGGATGTGGGTTTGTTCTTGAACGCCATTTCAACGGGCGCTGCGGGGTCGAAATCGCTGGATCTGTACGGGAGCAGGATATTGAAGAGGGATTTCGAAGCTGGGTTCTTCGTAAACCACTTTGTGAAGGATCTGGGAATCTGCCTGAAGGAGTGTCAGAATATGGGGATTGCTCTTCCGGGATTGGCATTGGCTCAGCAGCTTTATCTGTCGCTTAAGGCTCATGGGGAAGGGAATTTGGGCACTCAAGCGCTCATCTTATCCCTGGAGCGCCTCAACGACGTCTCGCTTCAATCTTCCACCGCCGCATCTTCT

Coding sequence (CDS)

ATGGCCACTTCCGGCGACATCCTCAGCCCCTCAACCACCCGCGTCGGCTGGATCGGCACCGGCGTCATGGGCTGCTCCATGTGCTCCCATCTCATCAGAGCCGGCTTCAAACTCACCGTCTTCAACCGCACAATTTCCAAAGCCCAGCCCCTCCTCGATCTGGGCGCCGATTTGGCCTCCTCCCCACTCGCCGTCGCCGCCCAATCCGACGTCGTCTTCTCCATCGTCGGCTACCCCTCCGACGTCCGCTCCGTTCTCCTCGACCCCACCTCCGGCGCCCTCGCCGGCCTCCGCCCCGGCGGCATCCTCGTCGACATGACCACTTCCGAGCCTTCCCTCGCATCCGAAATCGCCGCCGCCGCCGCCGCCAAGGGCTGCGGGGCGATCGACGCTCCAGTCTCCGGCGGCGATCGCGGCGCCAAGAACGCCACGCTGGCAATCTTCGCCGGCGGCGACGAGGGTCAAGTCCGGCGGCTGAATCCGTTGTTTGCCCTGTTGGGGAAGGTGAATTACATGGGAGAGAGCGGGAAAGGGCAGTTCGCGAAGCTGGCGAATCAGATCACCATAGCTTCGACGATGGTGGGGCTGGTGGAGGGGATGATTTATGCGCACAAAGCGGGTCTGGATGTGGGTTTGTTCTTGAACGCCATTTCAACGGGCGCTGCGGGGTCGAAATCGCTGGATCTGTACGGGAGCAGGATATTGAAGAGGGATTTCGAAGCTGGGTTCTTCGTAAACCACTTTGTGAAGGATCTGGGAATCTGCCTGAAGGAGTGTCAGAATATGGGGATTGCTCTTCCGGGATTGGCATTGGCTCAGCAGCTTTATCTGTCGCTTAAGGCTCATGGGGAAGGGAATTTGGGCACTCAAGCGCTCATCTTATCCCTGGAGCGCCTCAACGACGTCTCGCTTCAATCTTCCACCGCCGCATCTTCT

Protein sequence

MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLNDVSLQSSTAASS

Homology

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BLAST of MS023731 vs. NCBI nr
Match: XP_022154732.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Momordica charantia])

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-165
Identity = 311/312 (99.68%), Postives = 311/312 (99.68%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS
Sbjct: 29  MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 88

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA
Sbjct: 89  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 148

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
           AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF
Sbjct: 149 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 208

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE
Sbjct: 209 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 268

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
           AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN
Sbjct: 269 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 328

Query: 301 DVSLQSSTAASS 313
           DVSLQSS AASS
Sbjct: 329 DVSLQSSAAASS 340

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BLAST of MS023731 vs. NCBI nr
Match: KAG6599482.1 (putative 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030457.1 putative 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 561.6 bits (1446), Expect = 4.2e-156
Identity = 289/312 (92.63%), Postives = 305/312 (97.76%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           MATSGDI+SP+TTRVGWIGTGVMGCSMCSH+I+AGFKLTVFNRTISKAQPLLDLGA+LAS
Sbjct: 1   MATSGDIVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLAS 60

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGG+LVDMTTSEPSLASEIAAA
Sbjct: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAA 120

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
           AAAKGC A+DAPVSGGDRGAKNA+LAIFAGG+EG+VRRLNPLF+LLGKVNYMGESGKGQF
Sbjct: 121 AAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQF 180

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYAHKAGLDV LFLNAISTGAAGSKSLDLYGSRIL+RDFE
Sbjct: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFE 240

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
            GFFVNHFVKDLGICLKECQNMG+ALPGLALAQQLY+SLKAHGEGNLGTQALILSLERLN
Sbjct: 241 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLN 300

Query: 301 DVSLQSSTAASS 313
           +VSLQS+    S
Sbjct: 301 NVSLQSAARGGS 312

[Close]

BLAST of MS023731 vs. NCBI nr
Match: XP_022946035.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 560.1 bits (1442), Expect = 1.2e-155
Identity = 288/312 (92.31%), Postives = 304/312 (97.44%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           MATSGDI+SP+TTRVGWIGTGVMGCSMCSH+I+AGFKLTVFNRTISKAQPLLDLGA+LAS
Sbjct: 33  MATSGDIVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLAS 92

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGG+LVDMTTSEPSLASEIAAA
Sbjct: 93  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAA 152

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
            AAKGC A+DAPVSGGDRGAKNA+LAIFAGG+EG+VRRLNPLF+LLGKVNYMGESGKGQF
Sbjct: 153 TAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQF 212

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYAHKAGLDV LFLNAISTGAAGSKSLDLYGSRIL+RDFE
Sbjct: 213 AKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFE 272

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
            GFFVNHFVKDLGICLKECQNMG+ALPGLALAQQLY+SLKAHGEGNLGTQALILSLERLN
Sbjct: 273 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLN 332

Query: 301 DVSLQSSTAASS 313
           +VSLQS+    S
Sbjct: 333 NVSLQSAARGGS 344

[Close]

BLAST of MS023731 vs. NCBI nr
Match: XP_023545872.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.1 bits (1442), Expect = 1.2e-155
Identity = 288/312 (92.31%), Postives = 304/312 (97.44%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           M TSGDI+SPSTTRVGWIGTGVMGCSMCSH+I+AGFKLTVFNRTISKAQPLLDLGA+LAS
Sbjct: 33  MTTSGDIVSPSTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLAS 92

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGG+LVDMTTSEPSLASEIAAA
Sbjct: 93  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAA 152

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
           AAAKGC A+DAPVSGGDRGAKNA+LAIFAGG+EG+V+RLNPLF+LLGKVNYMGESGKGQF
Sbjct: 153 AAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVQRLNPLFSLLGKVNYMGESGKGQF 212

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYAHKAGLDV LFLNAISTGAAGSKSLDLYGSRIL+RDFE
Sbjct: 213 AKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFE 272

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
            GFFVNHFVKDLGICLKECQNMG+ALPGLALAQQLY+SLKAHGEGNLGTQALILSLERLN
Sbjct: 273 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLN 332

Query: 301 DVSLQSSTAASS 313
           +VSLQS+    S
Sbjct: 333 NVSLQSAAGGGS 344

[Close]

BLAST of MS023731 vs. NCBI nr
Match: XP_022999508.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 559.3 bits (1440), Expect = 2.1e-155
Identity = 287/312 (91.99%), Postives = 305/312 (97.76%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           MATSGDI++P+TTRVGWIGTGVMGCSMCSH+I+AGFKLTVFNRTISKAQPL+DLGA+LAS
Sbjct: 33  MATSGDIVNPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLVDLGANLAS 92

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGG+LVDMTTSEPSLASEIAAA
Sbjct: 93  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAA 152

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
           AAAKGC A+DAPVSGGDRGAKNA+LAIFAGG+EG+VRRLNPLF+LLGKVNYMGESGKGQF
Sbjct: 153 AAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQF 212

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYAHKAGLDV LFLNAISTGAAGSKSLDLYGSRIL+RDFE
Sbjct: 213 AKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFE 272

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
            GFFVNHFVKDLGICLKECQNMG+ALPGLALAQQLY+SLKAHGEGNLGTQALILSLERLN
Sbjct: 273 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLN 332

Query: 301 DVSLQSSTAASS 313
           +VSLQS+    S
Sbjct: 333 NVSLQSAARGGS 344

[Close]

BLAST of MS023731 vs. ExPASy Swiss-Prot
Match: Q9SZE1 (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 1.5e-132
Identity = 240/304 (78.95%), Postives = 274/304 (90.13%), Query Frame = 0


Query: 4   SGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPL 63
           S DI++PS T++GWIGTGVMG SMC HLI+AG+ +TVFNRTISKAQ L+D+GA++A SP 
Sbjct: 29  SSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPN 88

Query: 64  AVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAA 123
           +VA QSDVVF+IVGYPSDVR VLLDP SGAL+GLR GG+LVDMTTSEPSLA EIA AA+ 
Sbjct: 89  SVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASF 148

Query: 124 KGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQFAKL 183
           K C +IDAPVSGGD GAKN  L+IFAGGDE  V+RL+PLF+L+GKVN+MG SGKGQFAKL
Sbjct: 149 KNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKL 208

Query: 184 ANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGF 243
           ANQITIASTM+GLVEG+IYAHKAGLDV  FL AISTGAAGSKS+DLYG RILKRDF+ GF
Sbjct: 209 ANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGF 268

Query: 244 FVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLNDVS 303
           +VNHFVKDLGICL ECQ MG+ALPGLALAQQLYLSLKAHGEG+LGTQAL+L+LERLN+VS
Sbjct: 269 YVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLNNVS 328

Query: 304 LQSS 308
           +QSS
Sbjct: 329 VQSS 332

[Close]

BLAST of MS023731 vs. ExPASy Swiss-Prot
Match: Q949M8 (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 6.1e-73
Identity = 147/297 (49.49%), Postives = 200/297 (67.34%), Query Frame = 0


Query: 7   ILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVA 66
           ++ PS TR+GWIG G+MG +M SH+I AG+ +TV+ R + K + L   GA +A+SP  +A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 67  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGC 126
             SDVVF+IVG  +DVRS+LL    G L+GL PGG+ VDMT+S+P LA EI A A  + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 127 GAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQFAKLANQ 186
            A+DAPVSGGD GA+  TL IFAGGD   V  L+P+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 187 ITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVN 246
           I  AS +VGL EG+++A KAGLD   +L A+  GAAGS  + L+G  I+KRD+ A  F  
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 247 HFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLNDVS 304
           + VKDLG+  +       A+PG AL++QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

[Close]

BLAST of MS023731 vs. ExPASy Swiss-Prot
Match: Q9C991 (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 7.7e-68
Identity = 138/297 (46.46%), Postives = 194/297 (65.32%), Query Frame = 0


Query: 7   ILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVA 66
           ++ PS TR+GWIG G+MG +M SH++ AG+ +TV+ R + K + L   G   A+SP  + 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 67  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGC 126
             SDVVF+IVG  +DVRS+LL    G L+GL+PGG+ VDMT+S+P LA EI A A  + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 127 GAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQFAKLANQ 186
            A+DAPVSGGD GA+   L IFAGGD   V  L P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 187 ITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVN 246
           I + S M+GL EG+++A KAGLD   +L A+  GAAGS  + L+G  +  RD++A  F  
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 247 HFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLNDVS 304
           + VKDLG+  +      +A+PG AL +QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

[Close]

BLAST of MS023731 vs. ExPASy Swiss-Prot
Match: O34948 (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 GN=ykwC PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.3e-63
Identity = 131/279 (46.95%), Postives = 185/279 (66.31%), Query Frame = 0


Query: 15  VGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVAAQSDVVFS 74
           +G+IG GVMG SM SH++  G  + V+ RT  KA+ +L  GA    +   ++ ++DV+ +
Sbjct: 5   IGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADVIIT 64

Query: 75  IVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGCGAIDAPVS 134
           +VGYPSDV  V    ++G +   + G  L+DMTTS+PSLA +IA AA  K   A+DAPVS
Sbjct: 65  MVGYPSDVEEVYFG-SNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAPVS 124

Query: 135 GGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGK-VNYMGESGKGQFAKLANQITIASTM 194
           GGD GA+N TLAI  GG++       P+F+L+G+ + Y G +G GQ  K+ NQI IA+ M
Sbjct: 125 GGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAAGM 184

Query: 195 VGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVNHFVKDLG 254
           +G+ E M YA K+GL+    L +I+TGAAGS SL     R+L+ +FE GF+V HF+KD+G
Sbjct: 185 IGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIKDMG 244

Query: 255 ICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQAL 293
           I L+E + MG  +PGL+LA+ LY  L A GE N GTQ++
Sbjct: 245 IALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSI 282

[Close]

BLAST of MS023731 vs. ExPASy Swiss-Prot
Match: O33730 (Uncharacterized oxidoreductase Sfri_1503 OS=Shewanella frigidimarina (strain NCIMB 400) OX=318167 GN=Sfri_1503 PE=3 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 1.8e-40
Identity = 107/287 (37.28%), Postives = 152/287 (52.96%), Query Frame = 0


Query: 14  RVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKA-QPLLDLGADLASSPLAVAAQSDVV 73
           +V +IG GVMG  M  HL++ G  +TV+NRT +KA Q +   G   A +P   A   D+V
Sbjct: 3   KVAFIGLGVMGFPMAGHLVKQGHDVTVYNRTGAKATQWVEQYGGKKADTPKDAAQGQDIV 62

Query: 74  FSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGCGAIDAP 133
           F+ VG   D+R V+L    G + G+  G ILVD TT+   +A EIAA         +DAP
Sbjct: 63  FTCVGNDDDLRQVVLG-EHGIVHGMHAGAILVDHTTASADVAREIAAYIEPLNIAFLDAP 122

Query: 134 VSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGK-VNYMGESGKGQFAKLANQITIAS 193
           VSGG  GA+N  L +  GGD+     + P+ +   +    +G  G GQ  K+ NQI IA 
Sbjct: 123 VSGGQAGAENGALTVMMGGDQAHFDTVKPVISAYSRCAELLGPVGAGQLTKMVNQICIAG 182

Query: 194 TMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVNHFVKD 253
            + GL EG+ +A  AGLD    +  IS GAA S  ++     + +  ++ GF ++   KD
Sbjct: 183 VVQGLAEGLHFAKSAGLDGLKVIEVISKGAAQSWQMENRYKTMWQGQYDFGFAIDWMRKD 242

Query: 254 LGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLER 299
           LGI L E +  G  LP  AL  Q Y  ++A       T +L+  LE+
Sbjct: 243 LGIALDEARRNGSHLPVAALVDQFYSEVQAMKGNRWDTSSLLARLEK 288

[Close]

BLAST of MS023731 vs. ExPASy TrEMBL
Match: A0A6J1DMH9 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111021913 PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.3e-166
Identity = 311/312 (99.68%), Postives = 311/312 (99.68%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS
Sbjct: 29  MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 88

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA
Sbjct: 89  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 148

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
           AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF
Sbjct: 149 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 208

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE
Sbjct: 209 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 268

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
           AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN
Sbjct: 269 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 328

Query: 301 DVSLQSSTAASS 313
           DVSLQSS AASS
Sbjct: 329 DVSLQSSAAASS 340

[Close]

BLAST of MS023731 vs. ExPASy TrEMBL
Match: A0A6J1G2N8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111450245 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.9e-156
Identity = 288/312 (92.31%), Postives = 304/312 (97.44%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           MATSGDI+SP+TTRVGWIGTGVMGCSMCSH+I+AGFKLTVFNRTISKAQPLLDLGA+LAS
Sbjct: 33  MATSGDIVSPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLLDLGANLAS 92

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGG+LVDMTTSEPSLASEIAAA
Sbjct: 93  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAA 152

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
            AAKGC A+DAPVSGGDRGAKNA+LAIFAGG+EG+VRRLNPLF+LLGKVNYMGESGKGQF
Sbjct: 153 TAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQF 212

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYAHKAGLDV LFLNAISTGAAGSKSLDLYGSRIL+RDFE
Sbjct: 213 AKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFE 272

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
            GFFVNHFVKDLGICLKECQNMG+ALPGLALAQQLY+SLKAHGEGNLGTQALILSLERLN
Sbjct: 273 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLN 332

Query: 301 DVSLQSSTAASS 313
           +VSLQS+    S
Sbjct: 333 NVSLQSAARGGS 344

[Close]

BLAST of MS023731 vs. ExPASy TrEMBL
Match: A0A6J1KJW1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111493847 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.0e-155
Identity = 287/312 (91.99%), Postives = 305/312 (97.76%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           MATSGDI++P+TTRVGWIGTGVMGCSMCSH+I+AGFKLTVFNRTISKAQPL+DLGA+LAS
Sbjct: 33  MATSGDIVNPTTTRVGWIGTGVMGCSMCSHIIKAGFKLTVFNRTISKAQPLVDLGANLAS 92

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGG+LVDMTTSEPSLASEIAAA
Sbjct: 93  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAA 152

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
           AAAKGC A+DAPVSGGDRGAKNA+LAIFAGG+EG+VRRLNPLF+LLGKVNYMGESGKGQF
Sbjct: 153 AAAKGCKAVDAPVSGGDRGAKNASLAIFAGGNEGEVRRLNPLFSLLGKVNYMGESGKGQF 212

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYAHKAGLDV LFLNAISTGAAGSKSLDLYGSRIL+RDFE
Sbjct: 213 AKLANQITIASTMVGLVEGMIYAHKAGLDVSLFLNAISTGAAGSKSLDLYGSRILQRDFE 272

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
            GFFVNHFVKDLGICLKECQNMG+ALPGLALAQQLY+SLKAHGEGNLGTQALILSLERLN
Sbjct: 273 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLN 332

Query: 301 DVSLQSSTAASS 313
           +VSLQS+    S
Sbjct: 333 NVSLQSAARGGS 344

[Close]

BLAST of MS023731 vs. ExPASy TrEMBL
Match: A0A5D3CJZ2 (Putative 3-hydroxyisobutyrate dehydrogenase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00590 PE=4 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.9e-155
Identity = 286/304 (94.08%), Postives = 301/304 (99.01%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           M+TSGDI+SPSTTRVGWIGTGVMGCSMCSH+I+AG+KLTVFNRTISKAQPLLDLGA+LAS
Sbjct: 1   MSTSGDIVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLAS 60

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGG+LVDMTTSEPSLASEIAAA
Sbjct: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAA 120

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
           A AKGCGAIDAPVSGGDRGAKNATLAIFAGGDEG+VRRL+PLF+LLGKVNYMGESGKGQF
Sbjct: 121 ATAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGEVRRLSPLFSLLGKVNYMGESGKGQF 180

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYA KAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE
Sbjct: 181 AKLANQITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
            GFFVNHFVKDLGICLKECQNMG+ALPGLALAQQLY+SLKA+GEGNLGTQALILS+ERLN
Sbjct: 241 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSVERLN 300

Query: 301 DVSL 305
           +VSL
Sbjct: 301 NVSL 304

[Close]

BLAST of MS023731 vs. ExPASy TrEMBL
Match: A0A1S3C8W8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103498120 PE=4 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.9e-155
Identity = 286/304 (94.08%), Postives = 301/304 (99.01%), Query Frame = 0


Query: 1   MATSGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLAS 60
           M+TSGDI+SPSTTRVGWIGTGVMGCSMCSH+I+AG+KLTVFNRTISKAQPLLDLGA+LAS
Sbjct: 33  MSTSGDIVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLAS 92

Query: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAA 120
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGG+LVDMTTSEPSLASEIAAA
Sbjct: 93  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAA 152

Query: 121 AAAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQF 180
           A AKGCGAIDAPVSGGDRGAKNATLAIFAGGDEG+VRRL+PLF+LLGKVNYMGESGKGQF
Sbjct: 153 ATAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGEVRRLSPLFSLLGKVNYMGESGKGQF 212

Query: 181 AKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240
           AKLANQITIASTMVGLVEGMIYA KAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE
Sbjct: 213 AKLANQITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 272

Query: 241 AGFFVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLN 300
            GFFVNHFVKDLGICLKECQNMG+ALPGLALAQQLY+SLKA+GEGNLGTQALILS+ERLN
Sbjct: 273 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSVERLN 332

Query: 301 DVSL 305
           +VSL
Sbjct: 333 NVSL 336

[Close]

BLAST of MS023731 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 473.8 bits (1218), Expect = 1.1e-133
Identity = 240/304 (78.95%), Postives = 274/304 (90.13%), Query Frame = 0


Query: 4   SGDILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPL 63
           S DI++PS T++GWIGTGVMG SMC HLI+AG+ +TVFNRTISKAQ L+D+GA++A SP 
Sbjct: 29  SSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPN 88

Query: 64  AVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAA 123
           +VA QSDVVF+IVGYPSDVR VLLDP SGAL+GLR GG+LVDMTTSEPSLA EIA AA+ 
Sbjct: 89  SVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASF 148

Query: 124 KGCGAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQFAKL 183
           K C +IDAPVSGGD GAKN  L+IFAGGDE  V+RL+PLF+L+GKVN+MG SGKGQFAKL
Sbjct: 149 KNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKL 208

Query: 184 ANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGF 243
           ANQITIASTM+GLVEG+IYAHKAGLDV  FL AISTGAAGSKS+DLYG RILKRDF+ GF
Sbjct: 209 ANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGF 268

Query: 244 FVNHFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLNDVS 303
           +VNHFVKDLGICL ECQ MG+ALPGLALAQQLYLSLKAHGEG+LGTQAL+L+LERLN+VS
Sbjct: 269 YVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLNNVS 328

Query: 304 LQSS 308
           +QSS
Sbjct: 329 VQSS 332

[Close]

BLAST of MS023731 vs. TAIR 10
Match: AT1G71180.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 275.8 bits (704), Expect = 4.3e-74
Identity = 147/297 (49.49%), Postives = 200/297 (67.34%), Query Frame = 0


Query: 7   ILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVA 66
           ++ PS TR+GWIG G+MG +M SH+I AG+ +TV+ R + K + L   GA +A+SP  +A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 67  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGC 126
             SDVVF+IVG  +DVRS+LL    G L+GL PGG+ VDMT+S+P LA EI A A  + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 127 GAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQFAKLANQ 186
            A+DAPVSGGD GA+  TL IFAGGD   V  L+P+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 187 ITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVN 246
           I  AS +VGL EG+++A KAGLD   +L A+  GAAGS  + L+G  I+KRD+ A  F  
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 247 HFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLNDVS 304
           + VKDLG+  +       A+PG AL++QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

[Close]

BLAST of MS023731 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 258.8 bits (660), Expect = 5.5e-69
Identity = 138/297 (46.46%), Postives = 194/297 (65.32%), Query Frame = 0


Query: 7   ILSPSTTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVA 66
           ++ PS TR+GWIG G+MG +M SH++ AG+ +TV+ R + K + L   G   A+SP  + 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 67  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGC 126
             SDVVF+IVG  +DVRS+LL    G L+GL+PGG+ VDMT+S+P LA EI A A  + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 127 GAIDAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVNYMGESGKGQFAKLANQ 186
            A+DAPVSGGD GA+   L IFAGGD   V  L P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 187 ITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVN 246
           I + S M+GL EG+++A KAGLD   +L A+  GAAGS  + L+G  +  RD++A  F  
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 247 HFVKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLERLNDVS 304
           + VKDLG+  +      +A+PG AL +QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

[Close]

BLAST of MS023731 vs. TAIR 10
Match: AT1G17650.1 (glyoxylate reductase 2 )

HSP 1 Score: 141.4 bits (355), Expect = 1.3e-33
Identity = 93/289 (32.18%), Postives = 150/289 (51.90%), Query Frame = 0


Query: 12  TTRVGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVAAQSDV 71
           T  +G++G G+MG  M  +LI+AG  +TV+NRT SK  PL+ LGA   SSP  V A  D+
Sbjct: 66  TVSIGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDL 125

Query: 72  VFSIVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGCGA--I 131
            F+++  P     V     +GA+ G+  G   VD++T +  +AS I  +   K  GA  +
Sbjct: 126 TFAMLADPESAIDVACG-KNGAIFGISSGKGYVDVSTVD--VASSILISKQIKDTGALFL 185

Query: 132 DAPVSGGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVN-YMGESGKGQFAKLANQIT 191
           +APVSG  + A++  L     GD+    +  P   ++GK   Y+GE G G   KL   + 
Sbjct: 186 EAPVSGSKKPAEDGQLIFLTAGDKPLYEKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMI 245

Query: 192 IASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVNHF 251
           + S M    EG++ + K GLD  + +  +S GA  +    L G  ++K  +   F + H 
Sbjct: 246 MGSMMASFAEGILLSQKVGLDPNVLVEVVSQGAINAPMYSLKGPSMIKSVYPTAFPLKHQ 305

Query: 252 VKDLGICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALILSLE 298
            KD+ + L   +++  + P  A A +LY   K++G  +    A+I +L+
Sbjct: 306 QKDMRLALGLAESVSQSTPIAAAANELYKVAKSYGLSDEDFSAVIEALK 351

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BLAST of MS023731 vs. TAIR 10
Match: AT3G25530.1 (glyoxylate reductase 1 )

HSP 1 Score: 129.0 bits (323), Expect = 6.5e-30
Identity = 85/280 (30.36%), Postives = 141/280 (50.36%), Query Frame = 0


Query: 15  VGWIGTGVMGCSMCSHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVAAQSDVVFS 74
           VG++G G+MG +M  +L++ GFK+TV+NRT+SK   L++ GA +  SP  V  +     +
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 75  IVGYPSDVRSVLLDPTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGCGAIDAPVS 134
           ++  P    SV+ D   G L  +  G   +DM+T +   + +I  A   KG   ++ PVS
Sbjct: 63  MLSDPCAALSVVFD-KGGVLEQICEGKGYIDMSTVDAETSLKINEAITGKGGRFVEGPVS 122

Query: 135 GGDRGAKNATLAIFAGGDEGQVRRLNPLFALLGKVN-YMGESGKGQFAKLANQITIASTM 194
           G  + A++  L I A GD+       P F +LGK + Y+G+ G G   KL   + + S M
Sbjct: 123 GSKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMKLIVNMIMGSMM 182

Query: 195 VGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVNHFVKDLG 254
               EG++ A K+GL     L+ +  GA  +      G  + K  +   F + H  KD+ 
Sbjct: 183 NAFSEGLVLADKSGLSSDTLLDILDLGAMTNPMFKGKGPSMNKSSYPPAFPLKHQQKDMR 242

Query: 255 ICLKECQNMGIALPGLALAQQLYLSLKAHGEGNLGTQALI 294
           + L       +++P  A A + +   ++ G G+L   A+I
Sbjct: 243 LALALGDENAVSMPVAAAANEAFKKARSLGLGDLDFSAVI 281

The following BLAST results are available for this feature:

BLAST of MS023731 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154732.1	1.3e-165	99.68	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Momordica cha...	[more]
KAG6599482.1	4.2e-156	92.63	putative 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial, partial [Cucu...	[more]
XP_022946035.1	1.2e-155	92.31	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita mos...	[more]
XP_023545872.1	1.2e-155	92.31	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita pep...	[more]
XP_022999508.1	2.1e-155	91.99	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita max...	[more]

BLAST of MS023731 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZE1	1.5e-132	78.95	Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis...	[more]
Q949M8	6.1e-73	49.49	Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis...	[more]
Q9C991	7.7e-68	46.46	Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis...	[more]
O34948	3.3e-63	46.95	Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 ...	[more]
O33730	1.8e-40	37.28	Uncharacterized oxidoreductase Sfri_1503 OS=Shewanella frigidimarina (strain NCI...	[more]

BLAST of MS023731 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DMH9	6.3e-166	99.68	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Momordica c...	[more]
A0A6J1G2N8	5.9e-156	92.31	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita m...	[more]
A0A6J1KJW1	1.0e-155	91.99	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita m...	[more]
A0A5D3CJZ2	3.9e-155	94.08	Putative 3-hydroxyisobutyrate dehydrogenase-like 1 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3C8W8	3.9e-155	94.08	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucumis mel...	[more]

BLAST of MS023731 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29120.1	1.1e-133	78.95	6-phosphogluconate dehydrogenase family protein	[more]
AT1G71180.1	4.3e-74	49.49	6-phosphogluconate dehydrogenase family protein	[more]
AT1G71170.1	5.5e-69	46.46	6-phosphogluconate dehydrogenase family protein	[more]
AT1G17650.1	1.3e-33	32.18	glyoxylate reductase 2	[more]
AT3G25530.1	6.5e-30	30.36	glyoxylate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006115	6-phosphogluconate dehydrogenase, NADP-binding	PFAM	PF03446	NAD_binding_2	coord: 14..170 e-value: 5.5E-42 score: 143.6
IPR013328	6-phosphogluconate dehydrogenase, domain 2	GENE3D	1.10.1040.10		coord: 174..303 e-value: 1.3E-36 score: 127.7
IPR015815	3-hydroxyisobutyrate dehydrogenase-related	PIRSF	PIRSF000103	HIBADH	coord: 9..304 e-value: 6.5E-59 score: 197.4
IPR029154	3-hydroxyisobutyrate dehydrogenase, NAD-binding domain	PFAM	PF14833	NAD_binding_11	coord: 176..293 e-value: 1.3E-31 score: 109.3
None	No IPR available	GENE3D	3.40.50.720		coord: 8..173 e-value: 7.4E-47 score: 161.2
None	No IPR available	PANTHER	PTHR43060	3-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATED	coord: 8..301
IPR008927	6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily	SUPERFAMILY	48179	6-phosphogluconate dehydrogenase C-terminal domain-like	coord: 174..298
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 13..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023731.1	MS023731.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0050661	NADP binding
molecular_function	GO:0016491	oxidoreductase activity