MS023716 (gene) Bitter gourd (TR) v1

Overview
NameMS023716
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionInositol oxygenase
Locationscaffold570: 461901 .. 464715 (+)
RNA-Seq ExpressionMS023716
SyntenyMS023716
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAGAGGTTGAGGAGCAGAAGCTGTGTAATTTAGAAAGTGATAAGTTGGTATTGGACGGTGGGTCTGTAAATTCAAAACCTGCACCAAACGACGCCGCATTTGTCGCCCCCGAAATTAACTCTTTTGGCCATTCATTCAGGTTACCTTATGAATTATAATCTCATTTGATACGATTTTCATTTTGTTTATTATTTGTTTTCTTTAATCTTCGTCTATTTTTTTTTAATAAGAAGACCATTAAATTATCTTGACGTAATTTTTAATTATTATTTAACTTTAGTTAAAATTTTAGACACTATGCTGCTAGGAATTTAAAATGGAAAATCAAGAAGTAACAAATTAATTCATTATCCGCGATCCTCCCCCCCATTTTTTAATTTATTTGTTTTCTTAATTTTCGGCTGAAATTTATTGATTGTGTAAATATTTTTGTAGGGATTATGATGCAGAAAGTGAAAGGCAAAAAACCGTAGAAGAATTGTACCGCTTGAACCACATCAACCAAACCTATGATTTTGTAAGTTCGATTAGTTGATAACAAAACTAATTTTAAGTAAGATAATTTTTTTTGTGGGACTATTGGAAAACAGGTGAAGAGAATGAGAGAAGAATATGGGAAGTTGGAGAAGGTAGAAATGAGCATATGGGAATGCTGTGAGCTGCTGAACGAAGTAGTAGATGACAGTGATCCAGATTTGGATGAGCCTCAGATTCAGCACTTGCTTCAGTCTGCTGAAGCCATTAGAAAAGACTATCCCAATCAAGATTGGCTCCACTTGACTGCTCTCATCCATGGTACAATTTTCATTAATTATTTTTATTTTTGTTATATTTTTGTCTTAATATTTGGTCATTCTAGTCCCTCTAATTTCATAATGTTTTGATTCATTTAAATACTTGTACTTTCATGGTCTCTCTATTCTTAAAAGTGACTATTTTGGTTCATAGTTTCAAAATTCTAACACGACTTCTACCCATCACAAAAACTCTTTAATATAAAGTTATGGTTACATTTTAAGAAATATATTGAGTATTGTAGTGTTGTGTTGAAATTGTAATAGGAAAAATGAGATTAAAAATAAAATTAAGGGACGAGAATGATCACTTTTTAAAAATATAGGGATAAAAACAAAATTTTAAAAGTAAAAAAGTTAAAATGAACCAAAATTGAAAGTACCGGATCAAAATGAACATTTTAAGAGTACATGCACAAAAATAGTTATTTAAATTTTAATTTTTTTATAAAGTTTAAAATTTATGACATGTACTACATATATATATATGTTATTATTTATTTTACAATATTTGACTGTGTGTTTTGGTGGGGTTCTTTTAGACCTTGGAAAGGTTCTTCTTCTTCCTAGCTTTGGAGGGCTTCCTCAATGGGCTGTTGTTGGTAATATTATTCTTTTTGGCCCTACGGTTTATATAATTTTTTGAACCCTTTTTTCTCTCTTTATACAAGGGTTTGTTTTTTTTTTAATCTTCATGTAAATATTTTGTAGGTGATACACACCCTCTTGGCTGTGCTTTTGATGAATCTATTGTTCATCACAAGGTAGCAAAAACGTTAACCTCAAAATTTAACTTTCGTTCTTACTTCTTTTCTTTAATTTTTACAGAACCATGATTTTTTCATTGCATCGTAATGTTTTATGTTTATGATTTTTCATTTCTAAAAATTTGGGGAGAAACAAAATCTTCAGTGTTTTATAGAGCTAATTTCGAACGAACAATTTGCTTTTTTTTTTCTCCTGCTGCTCTGCCCTTCAATATAAATACAACCCAAAGTATTTCTATCAATTGAAAAACTAAACCAATGAAATTAAGTCCGTTTTATGTATTCTAGTTACCAACTTTTTAAGACGTTTTCAAAATCTAAGCCAAATTTTGAAAACTAATTTTCAAAAACTTATTTTTACTTTTACAATTTAGCTAAAAGTTAAACGTGTAATTAGAAAAGATATGTGAAAAAATTCTAAATAAATTGTGGGAAAACCAGCATAATTTTTAAAAACAGAAAACTAAAAACAAAATAAAGAGCCCCTAAATCATCAATAATCTAAGAACCAAAATTTAAAATAAAATTGTCATCAAGTAGTTTATGGAAAGAGATTTCTGATGGTTTTTTACCTTATATTTTGCAGTACTTCAAGGAGAATGTGGACTATGATAATCCATCTTATAACACCAAATATGGAATCTATTCTCAAGGGTGTGGCCTAGACAACGTAATGATTTCTTGGGGGCATGACGATTATATGTACTTGGTATTAAAATCAACCTTTTTTAGTATATTGTTTTTTTTTTCTTATGATCTTAAGATATATCAATTCAATTTCTGTTACTATATGAATTTGCATTCAAAATAATCCGTAGGTGGCTAAAGAGAATGGAACTACCCTACCTCCTGCTGGATTGTTTATCATTAGATATCATTCATTTTATCGTAAGTTTCCATTTCTTGGTATATAATATGTGAGGTAAAATAAATAATATGATTATTGATAATTTTATTTTATTGTAGCATTACACAGAGGAGGAGCATATGAACACTTGATGAACGAAGAGGATGTTGAGAATTTGAAGTGGCTCAAGATTTTCAAGTAATTTAATCAATCCCATTTCTTCAATTGGGGGTTTTTTTTTTAAATTTTTTTTAATCGTTTCAATGAAAAATTATTAAGTGATGTTAATATTTCTTTTGGAATGATCATGTTCAGCAAATACGACCTCTATAGCAAGAGCAAAGTTCTAGTCGACGTTGAAAAAGTGAAGCCTTACTATCAATCTCTTATTGAAAAGGTAAGTACT

mRNA sequence

TCAGAGGTTGAGGAGCAGAAGCTGTGTAATTTAGAAAGTGATAAGTTGGTATTGGACGGTGGGTCTGTAAATTCAAAACCTGCACCAAACGACGCCGCATTTGTCGCCCCCGAAATTAACTCTTTTGGCCATTCATTCAGGGATTATGATGCAGAAAGTGAAAGGCAAAAAACCGTAGAAGAATTGTACCGCTTGAACCACATCAACCAAACCTATGATTTTGTGAAGAGAATGAGAGAAGAATATGGGAAGTTGGAGAAGGTAGAAATGAGCATATGGGAATGCTGTGAGCTGCTGAACGAAGTAGTAGATGACAGTGATCCAGATTTGGATGAGCCTCAGATTCAGCACTTGCTTCAGTCTGCTGAAGCCATTAGAAAAGACTATCCCAATCAAGATTGGCTCCACTTGACTGCTCTCATCCATGACCTTGGAAAGGTTCTTCTTCTTCCTAGCTTTGGAGGGCTTCCTCAATGGGCTGTTGTTGGTGATACACACCCTCTTGGCTGTGCTTTTGATGAATCTATTGTTCATCACAAGTACTTCAAGGAGAATGTGGACTATGATAATCCATCTTATAACACCAAATATGGAATCTATTCTCAAGGGTGTGGCCTAGACAACGTAATGATTTCTTGGGGGCATGACGATTATATGTACTTGGTGGCTAAAGAGAATGGAACTACCCTACCTCCTGCTGGATTGTTTATCATTAGATATCATTCATTTTATCCATTACACAGAGGAGGAGCATATGAACACTTGATGAACGAAGAGGATGTTGAGAATTTGAAGTGGCTCAAGATTTTCAACAAATACGACCTCTATAGCAAGAGCAAAGTTCTAGTCGACGTTGAAAAAGTGAAGCCTTACTATCAATCTCTTATTGAAAAGGTAAGTACT

Coding sequence (CDS)

TCAGAGGTTGAGGAGCAGAAGCTGTGTAATTTAGAAAGTGATAAGTTGGTATTGGACGGTGGGTCTGTAAATTCAAAACCTGCACCAAACGACGCCGCATTTGTCGCCCCCGAAATTAACTCTTTTGGCCATTCATTCAGGGATTATGATGCAGAAAGTGAAAGGCAAAAAACCGTAGAAGAATTGTACCGCTTGAACCACATCAACCAAACCTATGATTTTGTGAAGAGAATGAGAGAAGAATATGGGAAGTTGGAGAAGGTAGAAATGAGCATATGGGAATGCTGTGAGCTGCTGAACGAAGTAGTAGATGACAGTGATCCAGATTTGGATGAGCCTCAGATTCAGCACTTGCTTCAGTCTGCTGAAGCCATTAGAAAAGACTATCCCAATCAAGATTGGCTCCACTTGACTGCTCTCATCCATGACCTTGGAAAGGTTCTTCTTCTTCCTAGCTTTGGAGGGCTTCCTCAATGGGCTGTTGTTGGTGATACACACCCTCTTGGCTGTGCTTTTGATGAATCTATTGTTCATCACAAGTACTTCAAGGAGAATGTGGACTATGATAATCCATCTTATAACACCAAATATGGAATCTATTCTCAAGGGTGTGGCCTAGACAACGTAATGATTTCTTGGGGGCATGACGATTATATGTACTTGGTGGCTAAAGAGAATGGAACTACCCTACCTCCTGCTGGATTGTTTATCATTAGATATCATTCATTTTATCCATTACACAGAGGAGGAGCATATGAACACTTGATGAACGAAGAGGATGTTGAGAATTTGAAGTGGCTCAAGATTTTCAACAAATACGACCTCTATAGCAAGAGCAAAGTTCTAGTCGACGTTGAAAAAGTGAAGCCTTACTATCAATCTCTTATTGAAAAGGTAAGTACT

Protein sequence

SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVEELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEKVST
Homology
BLAST of MS023716 vs. NCBI nr
Match: XP_022154748.1 (inositol oxygenase 4 [Momordica charantia])

HSP 1 Score: 624.8 bits (1610), Expect = 3.9e-175
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0

Query: 1   SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVE 60
           SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVE
Sbjct: 12  SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVE 71

Query: 61  ELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQ 120
           ELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQ
Sbjct: 72  ELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQ 131

Query: 121 SAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHK 180
           SAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHK
Sbjct: 132 SAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHK 191

Query: 181 YFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRY 240
           YFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRY
Sbjct: 192 YFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRY 251

Query: 241 HSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           HSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK
Sbjct: 252 HSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 309

BLAST of MS023716 vs. NCBI nr
Match: XP_038889701.1 (inositol oxygenase 2 [Benincasa hispida])

HSP 1 Score: 558.9 bits (1439), Expect = 2.6e-155
Identity = 265/298 (88.93%), Postives = 283/298 (94.97%), Query Frame = 0

Query: 1   SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVE 60
           SE E Q + +LESD+LVLDGG VN +  PNDAAF AP+INSFGHSFRDYDAESERQKTVE
Sbjct: 123 SEAESQSI-SLESDELVLDGGFVNPESLPNDAAFFAPKINSFGHSFRDYDAESERQKTVE 182

Query: 61  ELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQ 120
           ELYRLNHINQTYDFVKRMREEYGKL+K EMSIWECCE+LNEVVDDSDPDLDEPQIQHLLQ
Sbjct: 183 ELYRLNHINQTYDFVKRMREEYGKLDKTEMSIWECCEMLNEVVDDSDPDLDEPQIQHLLQ 242

Query: 121 SAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHK 180
           SAEAIRKDYPN+DWLHLTALIHDLGKVLLLP+FGGLPQWAVVGDT+PLGCAFD+SIVHHK
Sbjct: 243 SAEAIRKDYPNEDWLHLTALIHDLGKVLLLPAFGGLPQWAVVGDTYPLGCAFDKSIVHHK 302

Query: 181 YFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRY 240
           YFK+NVD D+P+YNTKYGIYS GCGLDNV+ISWGHDDYMYLVAKENGTTLP AGLFIIRY
Sbjct: 303 YFKDNVDNDDPNYNTKYGIYSPGCGLDNVVISWGHDDYMYLVAKENGTTLPHAGLFIIRY 362

Query: 241 HSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           HSFYPLH+ GAY+HLMNEEDVENLKWL IFNKYDLYSKSKVLVDVEKVKPYYQSLI+K
Sbjct: 363 HSFYPLHKEGAYQHLMNEEDVENLKWLHIFNKYDLYSKSKVLVDVEKVKPYYQSLIQK 419

BLAST of MS023716 vs. NCBI nr
Match: KAA0061508.1 (inositol oxygenase 2 [Cucumis melo var. makuwa] >TYK10765.1 inositol oxygenase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 539.7 bits (1389), Expect = 1.7e-149
Identity = 252/290 (86.90%), Postives = 275/290 (94.83%), Query Frame = 0

Query: 10  NLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYD-AESERQKTVEELYRLNHI 69
           +L+S++LVLDGG +N K +PNDA F APEINSFGHSFRDYD AES RQKTVEELYRLNHI
Sbjct: 20  SLDSNELVLDGGFINPKSSPNDAGFFAPEINSFGHSFRDYDNAESVRQKTVEELYRLNHI 79

Query: 70  NQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 129
           NQTYDFVKRMREEYGKL+K EMSIWECCE+LNEVVDDSDPDLDEPQIQHLLQSAEAIRKD
Sbjct: 80  NQTYDFVKRMREEYGKLDKTEMSIWECCEMLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 139

Query: 130 YPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDY 189
           YP++DWLHLTALIHDLGKVL+LP+FGGLPQWAVVGDT+PLGCAFD+SIVHHKYFK+N+D 
Sbjct: 140 YPDEDWLHLTALIHDLGKVLMLPAFGGLPQWAVVGDTYPLGCAFDKSIVHHKYFKDNIDS 199

Query: 190 DNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHR 249
            NP+YNTKYGIYS GCGLDNV+ISWGHDDYMYLVAKENGTTLP AGLFIIRYHSFYPLH+
Sbjct: 200 YNPAYNTKYGIYSPGCGLDNVVISWGHDDYMYLVAKENGTTLPDAGLFIIRYHSFYPLHK 259

Query: 250 GGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
            GAY+HLMN+ED EN+KWL IFNKYDLYSKSKVLVDVEKVKPYYQSLI+K
Sbjct: 260 EGAYQHLMNKEDAENMKWLHIFNKYDLYSKSKVLVDVEKVKPYYQSLIQK 309

BLAST of MS023716 vs. NCBI nr
Match: XP_008459136.1 (PREDICTED: inositol oxygenase 2 [Cucumis melo])

HSP 1 Score: 539.7 bits (1389), Expect = 1.7e-149
Identity = 252/290 (86.90%), Postives = 275/290 (94.83%), Query Frame = 0

Query: 10  NLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYD-AESERQKTVEELYRLNHI 69
           +L+S++LVLDGG +N K +PNDA F APEINSFGHSFRDYD AES RQKTVEELYRLNHI
Sbjct: 131 SLDSNELVLDGGFINPKSSPNDAGFFAPEINSFGHSFRDYDNAESVRQKTVEELYRLNHI 190

Query: 70  NQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 129
           NQTYDFVKRMREEYGKL+K EMSIWECCE+LNEVVDDSDPDLDEPQIQHLLQSAEAIRKD
Sbjct: 191 NQTYDFVKRMREEYGKLDKTEMSIWECCEMLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 250

Query: 130 YPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDY 189
           YP++DWLHLTALIHDLGKVL+LP+FGGLPQWAVVGDT+PLGCAFD+SIVHHKYFK+N+D 
Sbjct: 251 YPDEDWLHLTALIHDLGKVLMLPAFGGLPQWAVVGDTYPLGCAFDKSIVHHKYFKDNIDS 310

Query: 190 DNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHR 249
            NP+YNTKYGIYS GCGLDNV+ISWGHDDYMYLVAKENGTTLP AGLFIIRYHSFYPLH+
Sbjct: 311 YNPAYNTKYGIYSPGCGLDNVVISWGHDDYMYLVAKENGTTLPDAGLFIIRYHSFYPLHK 370

Query: 250 GGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
            GAY+HLMN+ED EN+KWL IFNKYDLYSKSKVLVDVEKVKPYYQSLI+K
Sbjct: 371 EGAYQHLMNKEDAENMKWLHIFNKYDLYSKSKVLVDVEKVKPYYQSLIQK 420

BLAST of MS023716 vs. NCBI nr
Match: XP_004141036.2 (inositol oxygenase 2 [Cucumis sativus] >KGN60583.2 hypothetical protein Csa_019332 [Cucumis sativus])

HSP 1 Score: 538.1 bits (1385), Expect = 4.8e-149
Identity = 250/290 (86.21%), Postives = 275/290 (94.83%), Query Frame = 0

Query: 10  NLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYD-AESERQKTVEELYRLNHI 69
           +L+S++LVLDG  VN K +PNDA F APEINSFGHSFRDYD AES RQKTVEELYRLNHI
Sbjct: 122 SLDSNELVLDGEFVNPKSSPNDAGFFAPEINSFGHSFRDYDNAESVRQKTVEELYRLNHI 181

Query: 70  NQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 129
           NQTYDFVK+MREEYGKL+K EMSIWECCE+LNEVVDDSDPDLDEPQIQHLLQSAEAIRKD
Sbjct: 182 NQTYDFVKKMREEYGKLDKTEMSIWECCEMLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 241

Query: 130 YPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDY 189
           YP++DWLHLTALIHDLGKVLLLP+FGGLPQWAVVGDT+PLGCAFD+SIVHHKYFK+N+D 
Sbjct: 242 YPDEDWLHLTALIHDLGKVLLLPAFGGLPQWAVVGDTYPLGCAFDKSIVHHKYFKDNIDN 301

Query: 190 DNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHR 249
           +NP+YNTKYG+YS GCGLDNV+ISWGHDDYMYLVAKENGTTLP AGLFI+RYHSFYP+H+
Sbjct: 302 NNPAYNTKYGVYSPGCGLDNVVISWGHDDYMYLVAKENGTTLPDAGLFIVRYHSFYPMHK 361

Query: 250 GGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
            GAY+HLMNEED EN+KWL IFNKYDLYSKSKVLVDVEKVKPYYQSLI+K
Sbjct: 362 EGAYQHLMNEEDAENMKWLHIFNKYDLYSKSKVLVDVEKVKPYYQSLIQK 411

BLAST of MS023716 vs. ExPASy Swiss-Prot
Match: Q8H1S0 (Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 3.5e-134
Identity = 227/285 (79.65%), Postives = 248/285 (87.02%), Query Frame = 0

Query: 15  KLVLDGG-SVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVEELYRLNHINQTYD 74
           +L LDGG S+      +D AF+APE+N+FG  FRDYD ESERQK VEE YRL HINQT D
Sbjct: 25  ELKLDGGFSMPKMDTNDDEAFLAPEMNAFGRQFRDYDVESERQKGVEEFYRLQHINQTVD 84

Query: 75  FVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDYPNQD 134
           FVK+MR EYGKL+K+ MSIWECCELLNEVVD+SDPDLDEPQIQHLLQSAEAIRKDYPN+D
Sbjct: 85  FVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDPDLDEPQIQHLLQSAEAIRKDYPNED 144

Query: 135 WLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDYDNPSY 194
           WLHLTALIHDLGKV+ LP FGGLPQWAVVGDT P+GCAFDES VHHKYF EN D+ N +Y
Sbjct: 145 WLHLTALIHDLGKVITLPQFGGLPQWAVVGDTFPVGCAFDESNVHHKYFVENPDFHNETY 204

Query: 195 NTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHRGGAYE 254
           NTK GIYS+GCGL+NVM+SWGHDDYMYLVAKENG+TLP AG FIIRYHSFYPLH  G Y 
Sbjct: 205 NTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENGSTLPSAGQFIIRYHSFYPLHTAGEYT 264

Query: 255 HLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           HLMNEED ENLKWL +FNKYDLYSKSKV VDVEKVKPYY SLI+K
Sbjct: 265 HLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEKVKPYYMSLIKK 309

BLAST of MS023716 vs. ExPASy Swiss-Prot
Match: O82200 (Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2)

HSP 1 Score: 474.9 bits (1221), Expect = 6.6e-133
Identity = 223/299 (74.58%), Postives = 258/299 (86.29%), Query Frame = 0

Query: 1   SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYD-AESERQKTV 60
           S V+E+K+     ++LVLDGG V  K    D AF AP++N  GHSFRDY+  ESERQ+ V
Sbjct: 12  SRVDEKKVIEERDNELVLDGGFVVPKSKETD-AFDAPDMNFLGHSFRDYENGESERQQGV 71

Query: 61  EELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLL 120
           EE YR+ HI+QTYDFVK+MR+EYGKL K+EMSIWECCELLN VVD+SDPDLDEPQIQHLL
Sbjct: 72  EEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVVDESDPDLDEPQIQHLL 131

Query: 121 QSAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHH 180
           Q+AEAIR+DYP++DWLHLTALIHDLGKVLLLP FGGLPQWAVVGDT P+GC FD + +HH
Sbjct: 132 QTAEAIRRDYPDEDWLHLTALIHDLGKVLLLPEFGGLPQWAVVGDTFPVGCTFDSANIHH 191

Query: 181 KYFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIR 240
           KYFK N D +NP YNTK G+Y++GCGLDNV++SWGHDDYMYLVAK+NGTTLP AGLFIIR
Sbjct: 192 KYFKGNHDINNPKYNTKNGVYTEGCGLDNVLMSWGHDDYMYLVAKKNGTTLPHAGLFIIR 251

Query: 241 YHSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           YHSFYPLH+ GAY HLMN+ED ++LKWL +FNKYDLYSKSKVLVDVE+VKPYY SLI K
Sbjct: 252 YHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLVDVEQVKPYYISLINK 309

BLAST of MS023716 vs. ExPASy Swiss-Prot
Match: Q8L799 (Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 3.4e-129
Identity = 213/268 (79.48%), Postives = 238/268 (88.81%), Query Frame = 0

Query: 31  DAAFVAPEINSFGHSFRDYDAESERQKTVEELYRLNHINQTYDFVKRMREEYGKLEKVEM 90
           DA F AP  NSFG +FRDYDAESER++ VEE YR+NHI QT DFV++MREEY KL + EM
Sbjct: 36  DAGFEAPHTNSFGRTFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEM 95

Query: 91  SIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDYPNQDWLHLTALIHDLGKVLLL 150
           SIWECCELLNE +D+SDPDLDEPQI+HLLQ+AEAIRKDYP++DWLHLT LIHDLGKVLL 
Sbjct: 96  SIWECCELLNEFIDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLH 155

Query: 151 PSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDYDNPSYNTKYGIYSQGCGLDNVM 210
            SFG LPQWAVVGDT P+GCAFDESIVHHKYFKEN DYDNPSYN+KYGIY++GCGLDNV+
Sbjct: 156 SSFGELPQWAVVGDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVL 215

Query: 211 ISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHRGGAYEHLMNEEDVENLKWLKIF 270
           +SWGHDDYMYLVAKEN TTLP AGLFIIRYHSFY LH+  AY+HLMN ED EN+KWLK+F
Sbjct: 216 MSWGHDDYMYLVAKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVF 275

Query: 271 NKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           NKYDLYSKSKV V+VE+VKPYY SL  K
Sbjct: 276 NKYDLYSKSKVRVNVEEVKPYYLSLTNK 303

BLAST of MS023716 vs. ExPASy Swiss-Prot
Match: Q9FJU4 (Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 3.4e-129
Identity = 217/288 (75.35%), Postives = 245/288 (85.07%), Query Frame = 0

Query: 12  ESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDY-DAESERQKTVEELYRLNHINQ 71
           ++ +L LD     SK + +D  F+APE+N+FG  FRDY D  SERQK+VE  Y   H NQ
Sbjct: 19  KTGELRLDSDIPMSKISSDDEVFLAPEMNAFGRQFRDYTDTNSERQKSVEHFYATQHTNQ 78

Query: 72  TYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDYP 131
           T DFV++MR EYGKL+K+ M+IWECCEL  EVVD+SDPDLDEPQIQHLLQSAEAIRKDYP
Sbjct: 79  TLDFVQKMRSEYGKLDKMVMNIWECCELSKEVVDESDPDLDEPQIQHLLQSAEAIRKDYP 138

Query: 132 NQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDYDN 191
           N+DWLHLTALIHDLGKVL LP FGGLPQWAVVGDT P+GCAFDES VHHKYF EN D++N
Sbjct: 139 NEDWLHLTALIHDLGKVLTLPQFGGLPQWAVVGDTFPVGCAFDESNVHHKYFMENPDFNN 198

Query: 192 PSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHRGG 251
           P YNTK GIYS+GCGL+NV++SWGHDDYMYLVAKENG+TLP  GLFIIRYHSFYPLH+ G
Sbjct: 199 PKYNTKAGIYSEGCGLENVLMSWGHDDYMYLVAKENGSTLPSPGLFIIRYHSFYPLHKAG 258

Query: 252 AYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           AY HLMNEED ENLKWL +FNKYDLYSKSKV V+VEKVKPYY SLI+K
Sbjct: 259 AYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVNVEKVKPYYMSLIKK 306

BLAST of MS023716 vs. ExPASy Swiss-Prot
Match: Q5Z8T3 (Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0561000 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 2.2e-128
Identity = 213/296 (71.96%), Postives = 248/296 (83.78%), Query Frame = 0

Query: 3   VEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVEEL 62
           +E+  L  +   K+   GG  N+     D  FV P+ N+FG++FR+Y+AESER++TVEE 
Sbjct: 5   IEQPHLDAIADRKVAGGGGGDNAAELVLDGGFVVPDSNAFGNAFRNYEAESERKETVEEF 64

Query: 63  YRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSA 122
           YR+NHINQTYDFV+RMREEYG+++K EM IWEC ELLNE +DDSDPDLD PQI+HLLQ+A
Sbjct: 65  YRVNHINQTYDFVRRMREEYGRVDKTEMGIWECIELLNEFIDDSDPDLDMPQIEHLLQTA 124

Query: 123 EAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYF 182
           EAIRKD+P++DWLHLT LIHDLGKVLL PSFG LPQW+VVGDT P+GCAFDE  VH KYF
Sbjct: 125 EAIRKDFPDEDWLHLTGLIHDLGKVLLHPSFGELPQWSVVGDTFPVGCAFDECNVHFKYF 184

Query: 183 KENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHS 242
           KEN DY NP  NTK+G YS+GCGLDNV++SWGHDDYMYLVAKEN TTLP AGLFIIRYHS
Sbjct: 185 KENPDYLNPKLNTKFGAYSEGCGLDNVLMSWGHDDYMYLVAKENKTTLPSAGLFIIRYHS 244

Query: 243 FYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           FYPLH+ GAY HLMN+ED ENLKWL++FNKYDLYSKS   +DVEKVKPYY SLIEK
Sbjct: 245 FYPLHKHGAYMHLMNDEDKENLKWLRVFNKYDLYSKSNERIDVEKVKPYYMSLIEK 300

BLAST of MS023716 vs. ExPASy TrEMBL
Match: A0A6J1DL60 (Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111021928 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 1.9e-175
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0

Query: 1   SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVE 60
           SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVE
Sbjct: 12  SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVE 71

Query: 61  ELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQ 120
           ELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQ
Sbjct: 72  ELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQ 131

Query: 121 SAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHK 180
           SAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHK
Sbjct: 132 SAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHK 191

Query: 181 YFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRY 240
           YFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRY
Sbjct: 192 YFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRY 251

Query: 241 HSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           HSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK
Sbjct: 252 HSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 309

BLAST of MS023716 vs. ExPASy TrEMBL
Match: A0A5D3CGX1 (Inositol oxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00780 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.0e-150
Identity = 252/290 (86.90%), Postives = 275/290 (94.83%), Query Frame = 0

Query: 10  NLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYD-AESERQKTVEELYRLNHI 69
           +L+S++LVLDGG +N K +PNDA F APEINSFGHSFRDYD AES RQKTVEELYRLNHI
Sbjct: 20  SLDSNELVLDGGFINPKSSPNDAGFFAPEINSFGHSFRDYDNAESVRQKTVEELYRLNHI 79

Query: 70  NQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 129
           NQTYDFVKRMREEYGKL+K EMSIWECCE+LNEVVDDSDPDLDEPQIQHLLQSAEAIRKD
Sbjct: 80  NQTYDFVKRMREEYGKLDKTEMSIWECCEMLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 139

Query: 130 YPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDY 189
           YP++DWLHLTALIHDLGKVL+LP+FGGLPQWAVVGDT+PLGCAFD+SIVHHKYFK+N+D 
Sbjct: 140 YPDEDWLHLTALIHDLGKVLMLPAFGGLPQWAVVGDTYPLGCAFDKSIVHHKYFKDNIDS 199

Query: 190 DNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHR 249
            NP+YNTKYGIYS GCGLDNV+ISWGHDDYMYLVAKENGTTLP AGLFIIRYHSFYPLH+
Sbjct: 200 YNPAYNTKYGIYSPGCGLDNVVISWGHDDYMYLVAKENGTTLPDAGLFIIRYHSFYPLHK 259

Query: 250 GGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
            GAY+HLMN+ED EN+KWL IFNKYDLYSKSKVLVDVEKVKPYYQSLI+K
Sbjct: 260 EGAYQHLMNKEDAENMKWLHIFNKYDLYSKSKVLVDVEKVKPYYQSLIQK 309

BLAST of MS023716 vs. ExPASy TrEMBL
Match: A0A1S3CAP6 (Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103498333 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.0e-150
Identity = 252/290 (86.90%), Postives = 275/290 (94.83%), Query Frame = 0

Query: 10  NLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYD-AESERQKTVEELYRLNHI 69
           +L+S++LVLDGG +N K +PNDA F APEINSFGHSFRDYD AES RQKTVEELYRLNHI
Sbjct: 131 SLDSNELVLDGGFINPKSSPNDAGFFAPEINSFGHSFRDYDNAESVRQKTVEELYRLNHI 190

Query: 70  NQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 129
           NQTYDFVKRMREEYGKL+K EMSIWECCE+LNEVVDDSDPDLDEPQIQHLLQSAEAIRKD
Sbjct: 191 NQTYDFVKRMREEYGKLDKTEMSIWECCEMLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 250

Query: 130 YPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDY 189
           YP++DWLHLTALIHDLGKVL+LP+FGGLPQWAVVGDT+PLGCAFD+SIVHHKYFK+N+D 
Sbjct: 251 YPDEDWLHLTALIHDLGKVLMLPAFGGLPQWAVVGDTYPLGCAFDKSIVHHKYFKDNIDS 310

Query: 190 DNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHR 249
            NP+YNTKYGIYS GCGLDNV+ISWGHDDYMYLVAKENGTTLP AGLFIIRYHSFYPLH+
Sbjct: 311 YNPAYNTKYGIYSPGCGLDNVVISWGHDDYMYLVAKENGTTLPDAGLFIIRYHSFYPLHK 370

Query: 250 GGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
            GAY+HLMN+ED EN+KWL IFNKYDLYSKSKVLVDVEKVKPYYQSLI+K
Sbjct: 371 EGAYQHLMNKEDAENMKWLHIFNKYDLYSKSKVLVDVEKVKPYYQSLIQK 420

BLAST of MS023716 vs. ExPASy TrEMBL
Match: A0A0A0LKP7 (Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G000640 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.3e-149
Identity = 250/290 (86.21%), Postives = 275/290 (94.83%), Query Frame = 0

Query: 10  NLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYD-AESERQKTVEELYRLNHI 69
           +L+S++LVLDG  VN K +PNDA F APEINSFGHSFRDYD AES RQKTVEELYRLNHI
Sbjct: 20  SLDSNELVLDGEFVNPKSSPNDAGFFAPEINSFGHSFRDYDNAESVRQKTVEELYRLNHI 79

Query: 70  NQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 129
           NQTYDFVK+MREEYGKL+K EMSIWECCE+LNEVVDDSDPDLDEPQIQHLLQSAEAIRKD
Sbjct: 80  NQTYDFVKKMREEYGKLDKTEMSIWECCEMLNEVVDDSDPDLDEPQIQHLLQSAEAIRKD 139

Query: 130 YPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDY 189
           YP++DWLHLTALIHDLGKVLLLP+FGGLPQWAVVGDT+PLGCAFD+SIVHHKYFK+N+D 
Sbjct: 140 YPDEDWLHLTALIHDLGKVLLLPAFGGLPQWAVVGDTYPLGCAFDKSIVHHKYFKDNIDN 199

Query: 190 DNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHR 249
           +NP+YNTKYG+YS GCGLDNV+ISWGHDDYMYLVAKENGTTLP AGLFI+RYHSFYP+H+
Sbjct: 200 NNPAYNTKYGVYSPGCGLDNVVISWGHDDYMYLVAKENGTTLPDAGLFIVRYHSFYPMHK 259

Query: 250 GGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
            GAY+HLMNEED EN+KWL IFNKYDLYSKSKVLVDVEKVKPYYQSLI+K
Sbjct: 260 EGAYQHLMNEEDAENMKWLHIFNKYDLYSKSKVLVDVEKVKPYYQSLIQK 309

BLAST of MS023716 vs. ExPASy TrEMBL
Match: A0A6J1KJU6 (Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111493835 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.0e-148
Identity = 249/289 (86.16%), Postives = 270/289 (93.43%), Query Frame = 0

Query: 10  NLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVEELYRLNHIN 69
           +LE ++  LDG   N + APNDA F+AP+INSFGHSFRDYDAES RQ+TVEELYRLNHIN
Sbjct: 149 SLECNESELDGEFENEEYAPNDAVFLAPKINSFGHSFRDYDAESARQRTVEELYRLNHIN 208

Query: 70  QTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDY 129
           QTYDFVKRMREEYGKL+K EMSIWECCELLNEVVD+SDPDLDEPQIQHLLQSAEAIRKDY
Sbjct: 209 QTYDFVKRMREEYGKLDKTEMSIWECCELLNEVVDESDPDLDEPQIQHLLQSAEAIRKDY 268

Query: 130 PNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDYD 189
           PN+DWLHLTALIHDLGKVLLLP FGGLPQWAVVGDT+PLGCAFD+SIVHHKYFK+N+D D
Sbjct: 269 PNEDWLHLTALIHDLGKVLLLPIFGGLPQWAVVGDTYPLGCAFDKSIVHHKYFKDNIDRD 328

Query: 190 NPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHRG 249
           NP+YNTKYGIYS GCGLDNV+ISWGHDDYMY+VAKENGTTLP AGLFIIRYHSFYP+H+ 
Sbjct: 329 NPAYNTKYGIYSHGCGLDNVVISWGHDDYMYMVAKENGTTLPSAGLFIIRYHSFYPMHQE 388

Query: 250 GAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           GAY HLMN+EDVENLKWL +FNKYDLYSKSKVLVDVEKVKPYYQSLI K
Sbjct: 389 GAYRHLMNKEDVENLKWLSVFNKYDLYSKSKVLVDVEKVKPYYQSLIRK 437

BLAST of MS023716 vs. TAIR 10
Match: AT4G26260.1 (myo-inositol oxygenase 4 )

HSP 1 Score: 479.2 bits (1232), Expect = 2.5e-135
Identity = 227/285 (79.65%), Postives = 248/285 (87.02%), Query Frame = 0

Query: 15  KLVLDGG-SVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVEELYRLNHINQTYD 74
           +L LDGG S+      +D AF+APE+N+FG  FRDYD ESERQK VEE YRL HINQT D
Sbjct: 25  ELKLDGGFSMPKMDTNDDEAFLAPEMNAFGRQFRDYDVESERQKGVEEFYRLQHINQTVD 84

Query: 75  FVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDYPNQD 134
           FVK+MR EYGKL+K+ MSIWECCELLNEVVD+SDPDLDEPQIQHLLQSAEAIRKDYPN+D
Sbjct: 85  FVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDPDLDEPQIQHLLQSAEAIRKDYPNED 144

Query: 135 WLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDYDNPSY 194
           WLHLTALIHDLGKV+ LP FGGLPQWAVVGDT P+GCAFDES VHHKYF EN D+ N +Y
Sbjct: 145 WLHLTALIHDLGKVITLPQFGGLPQWAVVGDTFPVGCAFDESNVHHKYFVENPDFHNETY 204

Query: 195 NTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHRGGAYE 254
           NTK GIYS+GCGL+NVM+SWGHDDYMYLVAKENG+TLP AG FIIRYHSFYPLH  G Y 
Sbjct: 205 NTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENGSTLPSAGQFIIRYHSFYPLHTAGEYT 264

Query: 255 HLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           HLMNEED ENLKWL +FNKYDLYSKSKV VDVEKVKPYY SLI+K
Sbjct: 265 HLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEKVKPYYMSLIKK 309

BLAST of MS023716 vs. TAIR 10
Match: AT4G26260.2 (myo-inositol oxygenase 4 )

HSP 1 Score: 479.2 bits (1232), Expect = 2.5e-135
Identity = 227/285 (79.65%), Postives = 248/285 (87.02%), Query Frame = 0

Query: 15  KLVLDGG-SVNSKPAPNDAAFVAPEINSFGHSFRDYDAESERQKTVEELYRLNHINQTYD 74
           +L LDGG S+      +D AF+APE+N+FG  FRDYD ESERQK VEE YRL HINQT D
Sbjct: 26  ELKLDGGFSMPKMDTNDDEAFLAPEMNAFGRQFRDYDVESERQKGVEEFYRLQHINQTVD 85

Query: 75  FVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDYPNQD 134
           FVK+MR EYGKL+K+ MSIWECCELLNEVVD+SDPDLDEPQIQHLLQSAEAIRKDYPN+D
Sbjct: 86  FVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDPDLDEPQIQHLLQSAEAIRKDYPNED 145

Query: 135 WLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDYDNPSY 194
           WLHLTALIHDLGKV+ LP FGGLPQWAVVGDT P+GCAFDES VHHKYF EN D+ N +Y
Sbjct: 146 WLHLTALIHDLGKVITLPQFGGLPQWAVVGDTFPVGCAFDESNVHHKYFVENPDFHNETY 205

Query: 195 NTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHRGGAYE 254
           NTK GIYS+GCGL+NVM+SWGHDDYMYLVAKENG+TLP AG FIIRYHSFYPLH  G Y 
Sbjct: 206 NTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENGSTLPSAGQFIIRYHSFYPLHTAGEYT 265

Query: 255 HLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           HLMNEED ENLKWL +FNKYDLYSKSKV VDVEKVKPYY SLI+K
Sbjct: 266 HLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEKVKPYYMSLIKK 310

BLAST of MS023716 vs. TAIR 10
Match: AT2G19800.1 (myo-inositol oxygenase 2 )

HSP 1 Score: 474.9 bits (1221), Expect = 4.7e-134
Identity = 223/299 (74.58%), Postives = 258/299 (86.29%), Query Frame = 0

Query: 1   SEVEEQKLCNLESDKLVLDGGSVNSKPAPNDAAFVAPEINSFGHSFRDYD-AESERQKTV 60
           S V+E+K+     ++LVLDGG V  K    D AF AP++N  GHSFRDY+  ESERQ+ V
Sbjct: 12  SRVDEKKVIEERDNELVLDGGFVVPKSKETD-AFDAPDMNFLGHSFRDYENGESERQQGV 71

Query: 61  EELYRLNHINQTYDFVKRMREEYGKLEKVEMSIWECCELLNEVVDDSDPDLDEPQIQHLL 120
           EE YR+ HI+QTYDFVK+MR+EYGKL K+EMSIWECCELLN VVD+SDPDLDEPQIQHLL
Sbjct: 72  EEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVVDESDPDLDEPQIQHLL 131

Query: 121 QSAEAIRKDYPNQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHH 180
           Q+AEAIR+DYP++DWLHLTALIHDLGKVLLLP FGGLPQWAVVGDT P+GC FD + +HH
Sbjct: 132 QTAEAIRRDYPDEDWLHLTALIHDLGKVLLLPEFGGLPQWAVVGDTFPVGCTFDSANIHH 191

Query: 181 KYFKENVDYDNPSYNTKYGIYSQGCGLDNVMISWGHDDYMYLVAKENGTTLPPAGLFIIR 240
           KYFK N D +NP YNTK G+Y++GCGLDNV++SWGHDDYMYLVAK+NGTTLP AGLFIIR
Sbjct: 192 KYFKGNHDINNPKYNTKNGVYTEGCGLDNVLMSWGHDDYMYLVAKKNGTTLPHAGLFIIR 251

Query: 241 YHSFYPLHRGGAYEHLMNEEDVENLKWLKIFNKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           YHSFYPLH+ GAY HLMN+ED ++LKWL +FNKYDLYSKSKVLVDVE+VKPYY SLI K
Sbjct: 252 YHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLVDVEQVKPYYISLINK 309

BLAST of MS023716 vs. TAIR 10
Match: AT1G14520.1 (myo-inositol oxygenase 1 )

HSP 1 Score: 462.6 bits (1189), Expect = 2.4e-130
Identity = 213/268 (79.48%), Postives = 238/268 (88.81%), Query Frame = 0

Query: 31  DAAFVAPEINSFGHSFRDYDAESERQKTVEELYRLNHINQTYDFVKRMREEYGKLEKVEM 90
           DA F AP  NSFG +FRDYDAESER++ VEE YR+NHI QT DFV++MREEY KL + EM
Sbjct: 36  DAGFEAPHTNSFGRTFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEM 95

Query: 91  SIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDYPNQDWLHLTALIHDLGKVLLL 150
           SIWECCELLNE +D+SDPDLDEPQI+HLLQ+AEAIRKDYP++DWLHLT LIHDLGKVLL 
Sbjct: 96  SIWECCELLNEFIDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLH 155

Query: 151 PSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDYDNPSYNTKYGIYSQGCGLDNVM 210
            SFG LPQWAVVGDT P+GCAFDESIVHHKYFKEN DYDNPSYN+KYGIY++GCGLDNV+
Sbjct: 156 SSFGELPQWAVVGDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVL 215

Query: 211 ISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHRGGAYEHLMNEEDVENLKWLKIF 270
           +SWGHDDYMYLVAKEN TTLP AGLFIIRYHSFY LH+  AY+HLMN ED EN+KWLK+F
Sbjct: 216 MSWGHDDYMYLVAKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVF 275

Query: 271 NKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           NKYDLYSKSKV V+VE+VKPYY SL  K
Sbjct: 276 NKYDLYSKSKVRVNVEEVKPYYLSLTNK 303

BLAST of MS023716 vs. TAIR 10
Match: AT1G14520.2 (myo-inositol oxygenase 1 )

HSP 1 Score: 462.6 bits (1189), Expect = 2.4e-130
Identity = 213/268 (79.48%), Postives = 238/268 (88.81%), Query Frame = 0

Query: 31  DAAFVAPEINSFGHSFRDYDAESERQKTVEELYRLNHINQTYDFVKRMREEYGKLEKVEM 90
           DA F AP  NSFG +FRDYDAESER++ VEE YR+NHI QT DFV++MREEY KL + EM
Sbjct: 34  DAGFEAPHTNSFGRTFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEM 93

Query: 91  SIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDYPNQDWLHLTALIHDLGKVLLL 150
           SIWECCELLNE +D+SDPDLDEPQI+HLLQ+AEAIRKDYP++DWLHLT LIHDLGKVLL 
Sbjct: 94  SIWECCELLNEFIDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLH 153

Query: 151 PSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENVDYDNPSYNTKYGIYSQGCGLDNVM 210
            SFG LPQWAVVGDT P+GCAFDESIVHHKYFKEN DYDNPSYN+KYGIY++GCGLDNV+
Sbjct: 154 SSFGELPQWAVVGDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVL 213

Query: 211 ISWGHDDYMYLVAKENGTTLPPAGLFIIRYHSFYPLHRGGAYEHLMNEEDVENLKWLKIF 270
           +SWGHDDYMYLVAKEN TTLP AGLFIIRYHSFY LH+  AY+HLMN ED EN+KWLK+F
Sbjct: 214 MSWGHDDYMYLVAKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVF 273

Query: 271 NKYDLYSKSKVLVDVEKVKPYYQSLIEK 299
           NKYDLYSKSKV V+VE+VKPYY SL  K
Sbjct: 274 NKYDLYSKSKVRVNVEEVKPYYLSLTNK 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154748.13.9e-175100.00inositol oxygenase 4 [Momordica charantia][more]
XP_038889701.12.6e-15588.93inositol oxygenase 2 [Benincasa hispida][more]
KAA0061508.11.7e-14986.90inositol oxygenase 2 [Cucumis melo var. makuwa] >TYK10765.1 inositol oxygenase 2... [more]
XP_008459136.11.7e-14986.90PREDICTED: inositol oxygenase 2 [Cucumis melo][more]
XP_004141036.24.8e-14986.21inositol oxygenase 2 [Cucumis sativus] >KGN60583.2 hypothetical protein Csa_0193... [more]
Match NameE-valueIdentityDescription
Q8H1S03.5e-13479.65Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1[more]
O822006.6e-13374.58Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2[more]
Q8L7993.4e-12979.48Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1[more]
Q9FJU43.4e-12975.35Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1[more]
Q5Z8T32.2e-12871.96Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g056... [more]
Match NameE-valueIdentityDescription
A0A6J1DL601.9e-175100.00Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111021928 PE=3 SV=1[more]
A0A5D3CGX18.0e-15086.90Inositol oxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G0... [more]
A0A1S3CAP68.0e-15086.90Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103498333 PE=3 SV=1[more]
A0A0A0LKP72.3e-14986.21Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G000640 PE=3 SV=1[more]
A0A6J1KJU62.0e-14886.16Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111493835 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G26260.12.5e-13579.65myo-inositol oxygenase 4 [more]
AT4G26260.22.5e-13579.65myo-inositol oxygenase 4 [more]
AT2G19800.14.7e-13474.58myo-inositol oxygenase 2 [more]
AT1G14520.12.4e-13079.48myo-inositol oxygenase 1 [more]
AT1G14520.22.4e-13079.48myo-inositol oxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007828Inositol oxygenasePFAMPF05153MIOXcoord: 57..298
e-value: 1.8E-119
score: 397.8
IPR007828Inositol oxygenasePANTHERPTHR12588MYOINOSITOL OXYGENASEcoord: 6..299
NoneNo IPR availablePANTHERPTHR12588:SF14INOSITOL OXYGENASE 5coord: 6..299
NoneNo IPR availableSUPERFAMILY109604HD-domain/PDEase-likecoord: 49..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023716.1MS023716.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019310 inositol catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050113 inositol oxygenase activity
molecular_function GO:0005506 iron ion binding