MS023678 (gene) Bitter gourd (TR) v1

Overview
NameMS023678
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionthermospermine synthase ACAULIS5-like
Locationscaffold570: 202703 .. 204867 (+)
RNA-Seq ExpressionMS023678
SyntenyMS023678
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTCATCCCTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACGCCAACGCCACTGCTCTCCCTCTCCAACATGACCGCCAACTCTCTCAATGGTACGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCTGTCAACAGGTCCATCCTTTCTCTCTATATGCCTTGCCTTTAACTGTAGGGTAGATAACACTAATTTTGAGCACAACCGATCTTCACATTCACTTCCTCTAGGATACTTCATGCCACGACCAGCGAGTTTCAAGATATAGTTCTCTTAGATACCAAACGATTTGGAAAGGTTTGGTTTATATCACACATCTTCTTCCTTACCAATAACACTTGATTAGTCCAAACGAGTTTACTGACACCATTTCAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTGCATCCTGCTCTGTTACTGCATCATAGGTATGCTGCCTTCCTTTTTGGTCGTGGGTCTGCAATCTGCAGCAAATCCGATTTTGTTCTTATCTTCCCTCTCGTCTCTTTGGATGATTTCAGCCCCAAAACAGTATTTATAATGGGAGGTGGAGAAGGGTCCACAGCTAGAGAGACGCTAAAGCACAAAGATATTGAGAAAGTTGTTATGTGTGATATTGATAGGGTATGGTTCTCAAACTCTTAAGAAAGATGCACTTATTTGTAATTTGTATCAATATGACCGAAAAATTAATAAGTAAAGAAGTGTTGATGGAAGAAAGGGAGGAGAACAAACCAATTGGTGCAATTGTCTTTCATTTGTGTTGTTACTGATAACCTTAGAGGGAACAACTTTGTAGGAGGTTGTCAATTTCTGCCATGCCCATCTGACCGAAAACCAGGATGCATTTCAGGACGAGAAACTTCACATTATTTTTGACGATGCAAAGTGAGTAGTTTTCCCTTTGGTCTATTATCCCATAAGCATGATTTCGATTTCCTTTTAATGATAGAGTTTCAGTAGGCACATGCCATAGTCCGTTCCATCTGCCATTTGCTATTAGTGCAATGTGTGTTATTGAGCTTGTCAAAGCTATATTTCTTGAACCAATGCATATTTCCCAAAGGATACAAGTTCTGTAATATCCATATGTCCAGTCGTCTAACCTAACCCACTCATAAATGAAACAGGGCTGGATTGGAGGGACAACCAGAGAAGTTCGATGTAATCATTGGCGATCTAGCGGATCCACACGAAGGAGGACCATGCAATCATCTTTACACTAAATCCTTCTATGAGGGAGTGATTAAGCCCAAGCTGAATGATAATGGTATATTTGTTACTCAGGTACATTATATTCTCAGTCCTAAATCTTCTCAACGCTATAGCAACCTCTCTCTGTTTTGTTCTTTATTCTCCTCTGTAAAATGTAGGCTGGCCCTGCTGGTATGCTCTCCCACAAGGTCTTCTCATCAATATACAACACTGTAAAGCATGTCTTCAGATGTAAGAATTTTGTTAGTGTAAGCCGTGATTCGTTTTATTATGCAATTCGACTGTATTTTATTGATGCGATCTTCTATATATAAATTGTATCAATATAAATATTGGTTGGCGTAACATGTTTTGTTTCATCTGCACAGATGTGATTGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGTAAGCAACTAGAAAGAATTTAATTCCTTGTAATTGCCTGAACTGAAGGGAAAAAGAGGAGAGATGAGACATTATTATATATGGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTTGTGATCATCCTCTTAAACTGGATGTGGAGGATCTGAACAACAGGATACGTGAGAGAGTCCAGGGCGAGCTACGCTACTTGGATGGTGCATTCATCGTATCATCAACGGTCATAAACAAGACTATTCGTACAACGTAAGTTCTCTACTAGATTGAATGAGCATTGATTTTTTGAAAACAAAACGAAATAGTTATCAAAGAGCACCTTAGCTTCCCCGTTAGATTGTATCTTGAGCATCGAGTTTTGGATGGTTATTTGTGTGTTGTGGGTGCAGGGTGAAGAATGAGACTCATGTGTTCACTGAGGAAGATGCAAGGTTCGCACATGGCCAAGGCCTC

mRNA sequence

ATGTTTTCATCCCTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACGCCAACGCCACTGCTCTCCCTCTCCAACATGACCGCCAACTCTCTCAATGGTACGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCTGTCAACAGGATACTTCATGCCACGACCAGCGAGTTTCAAGATATAGTTCTCTTAGATACCAAACGATTTGGAAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTGCATCCTGCTCTGTTACTGCATCATAGCCCCAAAACAGTATTTATAATGGGAGGTGGAGAAGGGTCCACAGCTAGAGAGACGCTAAAGCACAAAGATATTGAGAAAGTTGTTATGTGTGATATTGATAGGGAGGTTGTCAATTTCTGCCATGCCCATCTGACCGAAAACCAGGATGCATTTCAGGACGAGAAACTTCACATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACAACCAGAGAAGTTCGATGTAATCATTGGCGATCTAGCGGATCCACACGAAGGAGGACCATGCAATCATCTTTACACTAAATCCTTCTATGAGGGAGTGATTAAGCCCAAGCTGAATGATAATGGTATATTTGTTACTCAGGCTGGCCCTGCTGGTATGCTCTCCCACAAGGTCTTCTCATCAATATACAACACTGTAAAGCATGTCTTCAGATATGTGATTGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGACATTATTATATATGGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTTGTGATCATCCTCTTAAACTGGATGTGGAGGATCTGAACAACAGGATACGTGAGAGAGTCCAGGGCGAGCTACGCTACTTGGATGGTGCATTCATCGTATCATCAACGGTCATAAACAAGACTATTCGTACAACGGTGAAGAATGAGACTCATGTGTTCACTGAGGAAGATGCAAGGTTCGCACATGGCCAAGGCCTC

Coding sequence (CDS)

ATGTTTTCATCCCTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACGCCAACGCCACTGCTCTCCCTCTCCAACATGACCGCCAACTCTCTCAATGGTACGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCTGTCAACAGGATACTTCATGCCACGACCAGCGAGTTTCAAGATATAGTTCTCTTAGATACCAAACGATTTGGAAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTGCATCCTGCTCTGTTACTGCATCATAGCCCCAAAACAGTATTTATAATGGGAGGTGGAGAAGGGTCCACAGCTAGAGAGACGCTAAAGCACAAAGATATTGAGAAAGTTGTTATGTGTGATATTGATAGGGAGGTTGTCAATTTCTGCCATGCCCATCTGACCGAAAACCAGGATGCATTTCAGGACGAGAAACTTCACATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACAACCAGAGAAGTTCGATGTAATCATTGGCGATCTAGCGGATCCACACGAAGGAGGACCATGCAATCATCTTTACACTAAATCCTTCTATGAGGGAGTGATTAAGCCCAAGCTGAATGATAATGGTATATTTGTTACTCAGGCTGGCCCTGCTGGTATGCTCTCCCACAAGGTCTTCTCATCAATATACAACACTGTAAAGCATGTCTTCAGATATGTGATTGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGACATTATTATATATGGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTTGTGATCATCCTCTTAAACTGGATGTGGAGGATCTGAACAACAGGATACGTGAGAGAGTCCAGGGCGAGCTACGCTACTTGGATGGTGCATTCATCGTATCATCAACGGTCATAAACAAGACTATTCGTACAACGGTGAAGAATGAGACTCATGTGTTCACTGAGGAAGATGCAAGGTTCGCACATGGCCAAGGCCTC

Protein sequence

MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL
Homology
BLAST of MS023678 vs. NCBI nr
Match: XP_038890496.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 607.8 bits (1566), Expect = 5.8e-170
Identity = 295/351 (84.05%), Postives = 317/351 (90.31%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLFIP  N+T+LPL H  Q+S+W+EEQLEDDLKWSFAVNRILHA TSEFQ
Sbjct: 1   MFSSFCSALSVLFIP--NSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHKDIEKVVMCDIDR+VVNFC AHL ENQDAFQDEKLHIIFDDAKAGLEG+PEKFDV
Sbjct: 121 ETLKHKDIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKLNDNGIFVTQAGPAG+LSH+VFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHEVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVF+YVIAYTAHVPSYADSCGWVL                     CDHP++LD+EDLNNR
Sbjct: 241 HVFKYVIAYTAHVPSYADSCGWVLA--------------------CDHPIELDMEDLNNR 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           IRERV+GEL YLDGA+IVSSTV+NKTIR ++ NETH+FTEEDARFAHG+GL
Sbjct: 301 IRERVRGELHYLDGAYIVSSTVVNKTIRASMMNETHLFTEEDARFAHGRGL 329

BLAST of MS023678 vs. NCBI nr
Match: XP_004141174.1 (thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical protein Csa_019446 [Cucumis sativus])

HSP 1 Score: 605.5 bits (1560), Expect = 2.9e-169
Identity = 295/351 (84.05%), Postives = 315/351 (89.74%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLF+P  N+++LPL H +QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFVP--NSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEG TAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDE+LHIIFDDAKAGLEG+PEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVFRYVIAYTAHVPSYADSCGWVL                      DHP+KLD+EDLNN+
Sbjct: 241 HVFRYVIAYTAHVPSYADSCGWVLA--------------------SDHPIKLDIEDLNNK 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           IRERVQGEL YLDGAFIVSSTVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 IRERVQGELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

BLAST of MS023678 vs. NCBI nr
Match: XP_008459675.1 (PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa] >TYK10801.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa])

HSP 1 Score: 599.7 bits (1545), Expect = 1.6e-167
Identity = 294/351 (83.76%), Postives = 313/351 (89.17%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLFIP  N+++LPL H  QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFIP--NSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDEKLHIIFDDAKAGLEG+ EKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVF+YVIAYTAHVPSYADSCGWVL                      DHP++LD EDLNN+
Sbjct: 241 HVFKYVIAYTAHVPSYADSCGWVLA--------------------SDHPIRLDKEDLNNK 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           I ERV+GELRYLDGAFIVSSTVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 IHERVRGELRYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

BLAST of MS023678 vs. NCBI nr
Match: XP_022946737.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 575.5 bits (1482), Expect = 3.2e-160
Identity = 282/351 (80.34%), Postives = 303/351 (86.32%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S LCSAL  L IP +N+TA+P      +S+W+EEQLEDDLKWSFAVNRILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----HVSEWFEEQLEDDLKWSFAVNRILHAATSKFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHH+PKTVFI+GGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL+ENQDAFQDEKLHIIFDDAK GLEGQPEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           II DLADPHEGGPCNHLYTKSFYE  IKPKLNDNGIFVTQAGP+G+LS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVF+YVIAYTAH+PSYADS GWVL                      DHP KLD+EDL+ R
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLA--------------------SDHPFKLDIEDLDKR 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           IRERV+GELRYLDGAFIVSSTVINKTIRT + NETHVFTEEDARF HGQGL
Sbjct: 301 IRERVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVFTEEDARFTHGQGL 326

BLAST of MS023678 vs. NCBI nr
Match: KAG7030499.1 (Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 575.5 bits (1482), Expect = 3.2e-160
Identity = 282/351 (80.34%), Postives = 303/351 (86.32%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S LCSAL  L IP +N+TA+P      +S+W+EEQLEDDLKWSFAVNRILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----HVSEWFEEQLEDDLKWSFAVNRILHAATSKFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHH+PKTVFI+GGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL+ENQDAFQDEKLHIIFDDAK GLEGQPEKFD+
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDI 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           II DLADPHEGGPCNHLYTKSFYE  IKPKLNDNGIFVTQAGP+G+LS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVF+YVIAYTAH+PSYADS GWVL                      DHP KLDVEDL+ R
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLA--------------------SDHPFKLDVEDLDKR 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           IRERV+GELRYLDGAFIVSSTVINKTIRT + NETHVFTEEDARF HGQGL
Sbjct: 301 IRERVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVFTEEDARFTHGQGL 326

BLAST of MS023678 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.5e-115
Identity = 200/339 (59.00%), Postives = 250/339 (73.75%), Query Frame = 0

Query: 14  IPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQDIVLLDTKRFGKA 73
           I  A +    L  ++Q   WYEE ++DDLKWSFA+N +LH  TSE+QDI LLDTKRFGK 
Sbjct: 16  IHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

Query: 74  LLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVM 133
           L++DGK+QSAE+DEFIYHE L+HPALL H +PKTVFIMGGGEGS ARE LKH  IEKVVM
Sbjct: 76  LVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVM 135

Query: 134 CDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLADPHEGGP 193
           CDID+EVV+FC   LT N DAF ++KL ++  DAKA LE + EKFD+I+GDLADP EGGP
Sbjct: 136 CDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGP 195

Query: 194 CNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHK-VFSSIYNTVKHVFRYVIAYTAH 253
           C  LYTKSFY+ ++KPKL+ NGIFVTQAGPAG+ +HK VF+SIYNT+K VF+YV AYTAH
Sbjct: 196 CYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAH 255

Query: 254 VPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNRIRERVQGELRYL 313
           VPS+AD+ GWV+                      DH   ++V++++ RI ERV GEL YL
Sbjct: 256 VPSFADTWGWVMA--------------------SDHEFDVEVDEMDRRIEERVNGELMYL 315

Query: 314 DGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           +    VS+  +NKTI   ++ ET V++EE+ARF HG G+
Sbjct: 316 NAPSFVSAATLNKTISLALEKETEVYSEENARFIHGHGV 334

BLAST of MS023678 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 5.1e-52
Identity = 115/299 (38.46%), Postives = 171/299 (57.19%), Query Frame = 0

Query: 47  AVNRILHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPK 106
           A+ +++    + +Q  +L++  RFGKAL+LDGK+QS   DEFIYHE+LVHP LL  ++P+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 107 TVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCHAHLTE-NQDAFQDEKLHIIFD 166
            + I+GGGEG+T RE LKHK I+ V M DID  V++F   +L E +Q AF + K  ++ +
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 167 DAKAGLEGQPEKFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAG 226
           D    ++   EKFD ++ DL DP +  P   LYTK FYE V   +++  GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 227 MLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFX 286
             S + FS IYNT+KHVF+ V A   +VP++    G                    F + 
Sbjct: 200 -FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWG--------------------FVYA 259

Query: 287 CD--HPLKLDVEDLNNRIRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEED 343
            D  +P +   E++NNRI+ER+ G LR+ DG   ++   I K IR  ++ E  + T E+
Sbjct: 260 SDEVNPAEFSKEEINNRIKERIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTREN 294

BLAST of MS023678 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.1e-49
Identity = 117/305 (38.36%), Postives = 167/305 (54.75%), Query Frame = 0

Query: 48  VNRILHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKT 107
           + R++ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 108 VFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCHAHLTE-NQDAFQDEKLHIIFDD 167
           V I+GGGEG+T RE LKH  +EK VM DID E+V     H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 168 AKAGLEGQPEKFDVIIGDLADP-HEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAG 227
           A+A LE   E++DV+I DL DP  E  P   LYT  FY  ++K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMIL 200

Query: 228 MLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFX 287
           +  H+V   ++ TV+  FRYV +Y  H+P                   FF  F F     
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPG------------------FFLNFGFLLASD 260

Query: 288 CDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARF 347
              P       +  RIRER    LR+L   ++ +  V+ K +   ++ ET V T+++  +
Sbjct: 261 AFDPAAFSEGVIEARIRER-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFY 305

Query: 348 AHGQG 351
              +G
Sbjct: 321 VTPEG 305

BLAST of MS023678 vs. ExPASy Swiss-Prot
Match: Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.1e-49
Identity = 117/305 (38.36%), Postives = 167/305 (54.75%), Query Frame = 0

Query: 48  VNRILHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKT 107
           + R++ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 108 VFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCHAHLTE-NQDAFQDEKLHIIFDD 167
           V I+GGGEG+T RE LKH  +EK VM DID E+V     H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 168 AKAGLEGQPEKFDVIIGDLADP-HEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAG 227
           A+A LE   E++DV+I DL DP  E  P   LYT  FY  ++K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMIL 200

Query: 228 MLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFX 287
           +  H+V   ++ TV+  FRYV +Y  H+P                   FF  F F     
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPG------------------FFLNFGFLLASD 260

Query: 288 CDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARF 347
              P       +  RIRER    LR+L   ++ +  V+ K +   ++ ET V T+++  +
Sbjct: 261 AFDPAAFSEGVIEARIRER-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFY 305

Query: 348 AHGQG 351
              +G
Sbjct: 321 VTPEG 305

BLAST of MS023678 vs. ExPASy Swiss-Prot
Match: Q4JAZ8 (Polyamine aminopropyltransferase OS=Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) OX=330779 GN=speE PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.0e-48
Identity = 118/311 (37.94%), Postives = 167/311 (53.70%), Query Frame = 0

Query: 33  WYEEQLEDDLKWSFAVNRILHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHE 92
           W+ E        + A+ RIL    SEFQ ++L +  RFGKAL++DGK+QS   DEFIYHE
Sbjct: 6   WHLEWQTPQEFHAHAIKRILIEERSEFQRVILAELFRFGKALIIDGKIQSTLADEFIYHE 65

Query: 93  SLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCHAHLTE-N 152
           SLVHP L+    P+ + I+GGGEG+T RE L++K + KV M DID  V+ F   +L E +
Sbjct: 66  SLVHPLLISLEDPENILILGGGEGATLREALRYKSVRKVTMVDIDPVVIKFAKQNLQEWH 125

Query: 153 QDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKL 212
             +F D + ++I  D    ++   EK++ II DL DP +  P   LYT  FY   +K  +
Sbjct: 126 MGSFDDSRTNLIIGDGYKFVKETNEKYNAIILDLTDPIQDSPSQLLYTSEFYRD-LKSII 185

Query: 213 NDNGIFVTQAGPAGMLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLTLLYMDF 272
             NG  VTQA  +   S   F+ IY+T+K VF+ V A   +VPS+    G          
Sbjct: 186 TPNGGLVTQA-TSPSFSLDTFAIIYSTLKTVFKNVSAGITYVPSFDGLWG---------- 245

Query: 273 FFFFFFFFFFFFFXCDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSSTVINKTIRTTV 332
                   F +     +P  L+   +N+RI+ERV G LR+ DG        + K IR  +
Sbjct: 246 --------FIYASDVTNPAHLNRNQINDRIKERVTGSLRFYDGETHEMLFRVPKYIREKI 296

Query: 333 KNETHVFTEED 343
           +NE  V T E+
Sbjct: 306 ENEKRVSTREN 296

BLAST of MS023678 vs. ExPASy TrEMBL
Match: A0A0A0LET5 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.4e-169
Identity = 295/351 (84.05%), Postives = 315/351 (89.74%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLF+P  N+++LPL H +QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFVP--NSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEG TAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDE+LHIIFDDAKAGLEG+PEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVFRYVIAYTAHVPSYADSCGWVL                      DHP+KLD+EDLNN+
Sbjct: 241 HVFRYVIAYTAHVPSYADSCGWVLA--------------------SDHPIKLDIEDLNNK 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           IRERVQGEL YLDGAFIVSSTVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 IRERVQGELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

BLAST of MS023678 vs. ExPASy TrEMBL
Match: A0A5A7UZV1 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001170 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.6e-168
Identity = 294/351 (83.76%), Postives = 313/351 (89.17%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLFIP  N+++LPL H  QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFIP--NSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDEKLHIIFDDAKAGLEG+ EKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVF+YVIAYTAHVPSYADSCGWVL                      DHP++LD EDLNN+
Sbjct: 241 HVFKYVIAYTAHVPSYADSCGWVLA--------------------SDHPIRLDKEDLNNK 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           I ERV+GELRYLDGAFIVSSTVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 IHERVRGELRYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

BLAST of MS023678 vs. ExPASy TrEMBL
Match: A0A1S3CAR3 (thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.6e-168
Identity = 294/351 (83.76%), Postives = 313/351 (89.17%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLFIP  N+++LPL H  QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFIP--NSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDEKLHIIFDDAKAGLEG+ EKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVF+YVIAYTAHVPSYADSCGWVL                      DHP++LD EDLNN+
Sbjct: 241 HVFKYVIAYTAHVPSYADSCGWVLA--------------------SDHPIRLDKEDLNNK 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           I ERV+GELRYLDGAFIVSSTVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 IHERVRGELRYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

BLAST of MS023678 vs. ExPASy TrEMBL
Match: A0A6J1G4J8 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450716 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.5e-160
Identity = 282/351 (80.34%), Postives = 303/351 (86.32%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S LCSAL  L IP +N+TA+P      +S+W+EEQLEDDLKWSFAVNRILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----HVSEWFEEQLEDDLKWSFAVNRILHAATSKFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHH+PKTVFI+GGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL+ENQDAFQDEKLHIIFDDAK GLEGQPEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           II DLADPHEGGPCNHLYTKSFYE  IKPKLNDNGIFVTQAGP+G+LS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVF+YVIAYTAH+PSYADS GWVL                      DHP KLD+EDL+ R
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLA--------------------SDHPFKLDIEDLDKR 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           IRERV+GELRYLDGAFIVSSTVINKTIRT + NETHVFTEEDARF HGQGL
Sbjct: 301 IRERVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVFTEEDARFTHGQGL 326

BLAST of MS023678 vs. ExPASy TrEMBL
Match: A0A6J1KJ05 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111493662 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.8e-159
Identity = 280/351 (79.77%), Postives = 303/351 (86.32%), Query Frame = 0

Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S LCSAL  L IP +N+TA+P     Q+S+W+EEQLEDDLKWSFAV RILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----QVSEWFEEQLEDDLKWSFAVTRILHAATSKFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHH+PKTVFI+GGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL+ENQDAFQDEKLHIIF+DAK GLEGQPEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFNDAKVGLEGQPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           II DLADPHEGGPCNHLYTKSFYE  IKPKLNDNGIFVTQAGP+G+LS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNR 300
           HVF+YVIAYTAH+PSYADS GWVL                      DHP +LD+EDL+ R
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLA--------------------SDHPFELDMEDLDKR 300

Query: 301 IRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           IRERV+GELRYLDGAFIVSSTVINKTIRT + NETHVFTEEDARF HGQGL
Sbjct: 301 IRERVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVFTEEDARFTHGQGL 326

BLAST of MS023678 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 1.8e-116
Identity = 200/339 (59.00%), Postives = 250/339 (73.75%), Query Frame = 0

Query: 14  IPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQDIVLLDTKRFGKA 73
           I  A +    L  ++Q   WYEE ++DDLKWSFA+N +LH  TSE+QDI LLDTKRFGK 
Sbjct: 16  IHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

Query: 74  LLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVM 133
           L++DGK+QSAE+DEFIYHE L+HPALL H +PKTVFIMGGGEGS ARE LKH  IEKVVM
Sbjct: 76  LVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVM 135

Query: 134 CDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLADPHEGGP 193
           CDID+EVV+FC   LT N DAF ++KL ++  DAKA LE + EKFD+I+GDLADP EGGP
Sbjct: 136 CDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGP 195

Query: 194 CNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHK-VFSSIYNTVKHVFRYVIAYTAH 253
           C  LYTKSFY+ ++KPKL+ NGIFVTQAGPAG+ +HK VF+SIYNT+K VF+YV AYTAH
Sbjct: 196 CYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAH 255

Query: 254 VPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNRIRERVQGELRYL 313
           VPS+AD+ GWV+                      DH   ++V++++ RI ERV GEL YL
Sbjct: 256 VPSFADTWGWVMA--------------------SDHEFDVEVDEMDRRIEERVNGELMYL 315

Query: 314 DGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           +    VS+  +NKTI   ++ ET V++EE+ARF HG G+
Sbjct: 316 NAPSFVSAATLNKTISLALEKETEVYSEENARFIHGHGV 334

BLAST of MS023678 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 1.8e-116
Identity = 200/339 (59.00%), Postives = 250/339 (73.75%), Query Frame = 0

Query: 14  IPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQDIVLLDTKRFGKA 73
           I  A +    L  ++Q   WYEE ++DDLKWSFA+N +LH  TSE+QDI LLDTKRFGK 
Sbjct: 9   IHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 68

Query: 74  LLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVM 133
           L++DGK+QSAE+DEFIYHE L+HPALL H +PKTVFIMGGGEGS ARE LKH  IEKVVM
Sbjct: 69  LVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVM 128

Query: 134 CDIDREVVNFCHAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLADPHEGGP 193
           CDID+EVV+FC   LT N DAF ++KL ++  DAKA LE + EKFD+I+GDLADP EGGP
Sbjct: 129 CDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGP 188

Query: 194 CNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHK-VFSSIYNTVKHVFRYVIAYTAH 253
           C  LYTKSFY+ ++KPKL+ NGIFVTQAGPAG+ +HK VF+SIYNT+K VF+YV AYTAH
Sbjct: 189 CYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAH 248

Query: 254 VPSYADSCGWVLTLLYMDFFFFFFFFFFFFFFXCDHPLKLDVEDLNNRIRERVQGELRYL 313
           VPS+AD+ GWV+                      DH   ++V++++ RI ERV GEL YL
Sbjct: 249 VPSFADTWGWVMA--------------------SDHEFDVEVDEMDRRIEERVNGELMYL 308

Query: 314 DGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL 352
           +    VS+  +NKTI   ++ ET V++EE+ARF HG G+
Sbjct: 309 NAPSFVSAATLNKTISLALEKETEVYSEENARFIHGHGV 327

BLAST of MS023678 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 125.2 bits (313), Expect = 1.1e-28
Identity = 69/225 (30.67%), Postives = 118/225 (52.44%), Query Frame = 0

Query: 45  SFAVNRILHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHS 104
           S  V ++L    S++QD+++  +  +GK L+LDG +Q  E+DE  Y E + H  L    +
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 105 PKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIF 164
           PK V ++GGG+G   RE  +H  IE++ MC+ID+ VV+       +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 165 DDAKAGLEGQPE-KFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGP 224
            D  A L+   E  +D +I D +DP   GP   L+ K F++ V +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQA-E 284

Query: 225 AGMLSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCG-WVLTL 267
           +  L   +   I +  + +F+  + Y    VP+Y    G W+  L
Sbjct: 285 SLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYAL 325

BLAST of MS023678 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 122.9 bits (307), Expect = 5.3e-28
Identity = 67/214 (31.31%), Postives = 114/214 (53.27%), Query Frame = 0

Query: 45  SFAVNRILHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHS 104
           S  V +IL    S++QD+++  +  +GK L+LDG +Q  E+DE  Y E + H  L    +
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 105 PKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIF 164
           PK V ++GGG+G   RE  +H  +E++ +C+ID+ VV+    +       ++D ++++I 
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 165 DDAKAGLEGQPE-KFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGP 224
            D  A L+   E  +D +I D +DP   GP   L+ K F+E V +  L   G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFESVNR-ALRPGGVVCTQA-E 244

Query: 225 AGMLSHKVFSSIYNTVKHVFRYVIAYT-AHVPSY 257
           +  L   +   I +  + +F+  + Y    VP+Y
Sbjct: 245 SLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTY 274

BLAST of MS023678 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 122.9 bits (307), Expect = 5.3e-28
Identity = 66/214 (30.84%), Postives = 114/214 (53.27%), Query Frame = 0

Query: 45  SFAVNRILHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHS 104
           S  V ++L    S++QD+++  +  +GK L+LDG +Q  E+DE  Y E + H  L    +
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 105 PKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCHAHLTENQDAFQDEKLHIIF 164
           PK V ++GGG+G   RE  +H  IE++ MC+ID+ VV+       +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 165 DDAKAGLEGQPE-KFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGP 224
            D  A L+   E  +D +I D +DP   GP   L+ K F++ V +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQA-E 284

Query: 225 AGMLSHKVFSSIYNTVKHVFRYVIAYT-AHVPSY 257
           +  L   +   I +  + +F+  + Y    VP+Y
Sbjct: 285 SLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTY 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890496.15.8e-17084.05thermospermine synthase ACAULIS5-like [Benincasa hispida][more]
XP_004141174.12.9e-16984.05thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical prot... [more]
XP_008459675.11.6e-16783.76PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 th... [more]
XP_022946737.13.2e-16080.34thermospermine synthase ACAULIS5-like [Cucurbita moschata][more]
KAG7030499.13.2e-16080.34Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9S7X62.5e-11559.00Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV... [more]
Q975S55.1e-5238.46Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ... [more]
Q72K553.1e-4938.36Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ... [more]
Q5SK283.1e-4938.36Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS... [more]
Q4JAZ82.0e-4837.94Polyamine aminopropyltransferase OS=Sulfolobus acidocaldarius (strain ATCC 33909... [more]
Match NameE-valueIdentityDescription
A0A0A0LET51.4e-16984.05PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 S... [more]
A0A5A7UZV17.6e-16883.76Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CAR37.6e-16883.76thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE... [more]
A0A6J1G4J81.5e-16080.34thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450... [more]
A0A6J1KJ053.8e-15979.77thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149366... [more]
Match NameE-valueIdentityDescription
AT5G19530.11.8e-11659.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G19530.21.8e-11659.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23820.21.1e-2830.67spermidine synthase 1 [more]
AT1G70310.15.3e-2831.31spermidine synthase 2 [more]
AT1G23820.15.3e-2830.84spermidine synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 87..258
e-value: 3.3E-36
score: 124.5
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 84..349
e-value: 8.7E-68
score: 230.5
NoneNo IPR availablePANTHERPTHR43317THERMOSPERMINE SYNTHASE ACAULIS5coord: 18..265
NoneNo IPR availablePANTHERPTHR43317THERMOSPERMINE SYNTHASE ACAULIS5coord: 285..349
NoneNo IPR availablePANTHERPTHR43317:SF6THERMOSPERMINE SYNTHASE ACAULIS5-LIKEcoord: 18..265
coord: 285..349
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 112..220
e-value: 4.03511E-5
score: 40.4911
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 32..84
e-value: 1.5E-12
score: 47.2
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 30..83
e-value: 4.9E-16
score: 60.6
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 32..331
score: 29.635651
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 31..269
score: 84.930565
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 31..329

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023678.1MS023678.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity