MS023672 (gene) Bitter gourd (TR) v1

Overview


Name	MS023672
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Mediator of RNA polymerase II transcription subunit 20
Location	scaffold570: 178401 .. 180383 (+)
RNA-Seq Expression	MS023672
Synteny	MS023672

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGCTAGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATCCTCAACGAAGTTTCTCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACTCTCACCTTTTACAGGCCCATCTTACGAGGTAATTGCACTCTTTTCATAGAGTCGTTTGTAAGAATATCTTCTTTTTTTCTTTTTCTCGAGAGGGGGCTGTGGCCGTCGGGATTTAATTACCCGAATGAATCTTAAATCTGTCAGCAGACCAAAACTTACCGGCGGCCGAGTTCCCGCGCGACTTTCTCGGAATCTCGCTGCCGGAGCAACCAAACAAGTATTATCTTATAATCCGTGGCCAACGGATCGTCTTAGAAGCTGATTTTACGATTCAAACTATAATGGAGAAGTTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGTTCAACACCAAACTTTTCTAGTTCTCATGTAAACTGCACTTTCCCATGTCCAGAAATTCAAAATTGAAGTTGATTTTTGTGGCTTTCTGGTGTCTGATCTTTTGCCCTTTTGTTTTAGTTCGTGTGAGATCGATAAATCTTTGGTTACAAACAGTTTACCTTATTATTTTTTAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACCATTCTGAGAATTTGAGAGGAATAGTTATGGAGGTAAGGCTCATTTCATCATTTCATGATAACTCTTTTCTTGTCTGTTAACAATTGCATGCTCTTCTTATTGGTTTTTTATGTCAATATTCTTTATCTGAGCGAGCAGTTTCTTTCCCATGGGCTGTGTCAAAACAATGTTTGGTACAGGAAGTATTGATTCAACTTATCTTAATGCTTAACATGGCCTCTGTTGTTCTCATTTTGATAAACAATGAAGTGGAAGAAAAAGGCCAATCATCTGATAGAAGTATCAACTATCATTTTCTCTGAAACGTTTATGTAAGGTCGCGATCTTATGGTGAGAAGTAGTGAGATCTAGTTAATATATCCTTGCAAGCCTGTGTAAGAGTAGTCATGGAAGTCGTTATTTGGCCTTGCCCATCTTTATTATTGTTGCACATGAATTAGTTAATTACTCTCAAATAGGTGATTTTATTCAGTTTGGAAAGTGTCTTTCAATTCATTTGGATGCTTGGAAAGAGGGTTTGGTCGTTCCTTGGGGTGGACTTCATTGTTGTGCTCATCCTTAAACGGAATAAAGCGAGATAGACTGCCTATCACCTTTCAATCATCTGTTCTTGTTATCCTTTTTCCCCTTCCATATAGTTCTTCAAAAACACATTGCCATGTTCTTTATTGTATTGTCAAGTCCAAGTCCAAATAGAATATGCTAGCAACTAAGCAACTGTGATGAAGCCTCCCTCCGAGAATGGAGCTGAAGTTGGAAATCTGAAACTTCTCGAGCAAATTTATGGTTCAGTTCTGTAATTTGGTTTTAACCTACCCTAATGTATATCATATTGGATGTATTGTATTGTTAGTTCTGGGAAGTAAGAATCATATTTAAGTGGGATTTAATTAGAACATAGAATCTCTGTCAAATGTGCGCATGTTAGATTTTTGTGCTGCAATTACGAAAATTGCTTTTAGCTGCTGTTTGAATGGAGGACCATTTAGGACAGTCCAGATCATATATTGCTCGTGTCATAGTTATGCAATAATGTACTAAAATCCTGTATATTGCAGATGGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTGATGGAGGAATTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCATTGCCAGGTCGTTTCATGCACATAGAACCAAACTTTGCAGAATATGGACTGTCGGATTACTACACTCCTCAACACACAGCCGTCCAGTATGCTAATGTTATGGCGCAACTAATCGCAACAGTTCAAGCAGTTCAATCAGTGAGAAAT

mRNA sequence

TTGGCTAGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATCCTCAACGAAGTTTCTCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACTCTCACCTTTTACAGGCCCATCTTACGAGCAGACCAAAACTTACCGGCGGCCGAGTTCCCGCGCGACTTTCTCGGAATCTCGCTGCCGGAGCAACCAAACAAGTATTATCTTATAATCCGTGGCCAACGGATCGTCTTAGAAGCTGATTTTACGATTCAAACTATAATGGAGAAGTTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACCATTCTGAGAATTTGAGAGGAATAGTTATGGAGATGGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTGATGGAGGAATTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCATTGCCAGGTCGTTTCATGCACATAGAACCAAACTTTGCAGAATATGGACTGTCGGATTACTACACTCCTCAACACACAGCCGTCCAGTATGCTAATGTTATGGCGCAACTAATCGCAACAGTTCAAGCAGTTCAATCAGTGAGAAAT

Coding sequence (CDS)

TTGGCTAGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATCCTCAACGAAGTTTCTCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACTCTCACCTTTTACAGGCCCATCTTACGAGCAGACCAAAACTTACCGGCGGCCGAGTTCCCGCGCGACTTTCTCGGAATCTCGCTGCCGGAGCAACCAAACAAGTATTATCTTATAATCCGTGGCCAACGGATCGTCTTAGAAGCTGATTTTACGATTCAAACTATAATGGAGAAGTTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACCATTCTGAGAATTTGAGAGGAATAGTTATGGAGATGGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTGATGGAGGAATTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCATTGCCAGGTCGTTTCATGCACATAGAACCAAACTTTGCAGAATATGGACTGTCGGATTACTACACTCCTCAACACACAGCCGTCCAGTATGCTAATGTTATGGCGCAACTAATCGCAACAGTTCAAGCAGTTCAATCAGTGAGAAAT

Protein sequence

LARVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN

Homology

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BLAST of MS023672 vs. NCBI nr
Match: XP_038889134.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 422.2 bits (1084), Expect = 2.8e-114
Identity = 211/219 (96.35%), Postives = 215/219 (98.17%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR DQNLPAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNLPAAEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGISLPEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKSR+VMEEFFEVWQEAMSKSSLPGRFMHIEP
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRKVMEEFFEVWQEAMSKSSLPGRFMHIEP 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSDYYT QHTAVQYANVMAQLIATVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023672 vs. NCBI nr
Match: XP_008459711.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo])

HSP 1 Score: 415.6 bits (1067), Expect = 2.7e-112
Identity = 206/219 (94.06%), Postives = 213/219 (97.26%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR DQN PAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGISLPEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIES 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSD+YTPQHTAVQY NVMAQLIATVQAVQS RN
Sbjct: 186 NFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023672 vs. NCBI nr
Match: XP_004141069.1 (mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60509.1 hypothetical protein Csa_019466 [Cucumis sativus])

HSP 1 Score: 415.6 bits (1067), Expect = 2.7e-112
Identity = 208/219 (94.98%), Postives = 212/219 (96.80%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR DQN PAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGISLPEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKSRQVMEEFF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIES 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSDYYT QHTAVQY NVMAQLIATVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023672 vs. NCBI nr
Match: KAA0061469.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo var. makuwa] >TYK10805.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 415.6 bits (1067), Expect = 2.7e-112
Identity = 206/219 (94.06%), Postives = 213/219 (97.26%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR DQN PAAEFPRD
Sbjct: 54  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 113

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGISLPEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 114 FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 173

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 174 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIES 233

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSD+YTPQHTAVQY NVMAQLIATVQAVQS RN
Sbjct: 234 NFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of MS023672 vs. NCBI nr
Match: XP_022946745.1 (mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata] >XP_022999230.1 mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita maxima] >XP_023546929.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546930.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6599532.1 Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 413.7 bits (1062), Expect = 1.0e-111
Identity = 206/219 (94.06%), Postives = 212/219 (96.80%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR D N+ A EFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DTNIAAGEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGIS+PEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRFMHIEP
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEP 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSDYYT QHTAVQYANVMAQLIATVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023672 vs. ExPASy Swiss-Prot
Match: Q6NPF4 (Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana OX=3702 GN=MED20A PE=1 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-87
Identity = 158/215 (73.49%), Postives = 187/215 (86.98%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +Y+P+L+   N    EFPRD
Sbjct: 6   VLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQAN--QLEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGISL +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+GFQYQLGDF+L
Sbjct: 66  FLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLGDFRL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP HSEN+RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F++I+ 
Sbjct: 126 RVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFVNIDL 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQ 219
           NF E+GL D YTPQHTAV+YA VMA +IATVQAV+
Sbjct: 186 NFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of MS023672 vs. ExPASy Swiss-Prot
Match: Q9M0S0 (Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana OX=3702 GN=MED20B PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 7.6e-78
Identity = 138/213 (64.79%), Postives = 176/213 (82.63%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           +LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +Y+P+L+   N   AEFPR+
Sbjct: 6   LLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQAN--QAEFPRE 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           F+GISLPE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FEG +YQLGDF+L
Sbjct: 66  FVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLGDFRL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP H+E +RG+VME+EYLPISSM  ++++MEEF E+WQEAMSK SLPG+F++ E 
Sbjct: 126 RVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFVNKEL 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQA 217
           NF ++GL D YTPQHTAV YA  MA L+A +QA
Sbjct: 186 NFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of MS023672 vs. ExPASy Swiss-Prot
Match: Q54FL3 (Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium discoideum OX=44689 GN=med20 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.4e-09
Identity = 55/197 (27.92%), Postives = 91/197 (46.19%), Query Frame = 0


Query: 20  LNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRDFLGISLPEQPNKYYLI 79
           L E+++ +E + G+K        + Y   +    NL     PR+F  +   E+P K + +
Sbjct: 13  LPEMTKRIEFLGGIKVSTGTINCSLYLERVPDGSNL----IPREFHLLLYDEKPKKCFTV 72

Query: 80  IRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNHSENLRGI 139
            R    ++E D  + +I+EK  SYK R        +Y+LGDF +RVG +V      +RGI
Sbjct: 73  CRD--TIVETDREMVSILEKTNSYKKRQTTEALISKYELGDFIIRVGPIV--FRTEIRGI 132

Query: 140 VMEMEY----LPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDY-- 199
           ++E+EY     P++S     +++ EF          +   G F   EP    +  S Y  
Sbjct: 133 LVEVEYTACSTPVTSPFNCTKLLSEFI---------NGNLGPFP--EPIQTSFDFSAYPT 190

Query: 200 ----YTPQHTAVQYANV 207
               Y+  HT+ QY N+
Sbjct: 193 LPKSYSDLHTSYQYVNL 190

[Close]

BLAST of MS023672 vs. ExPASy TrEMBL
Match: A0A5D3CH02 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. makuwa OX=1194695 GN=MED20 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.3e-112
Identity = 206/219 (94.06%), Postives = 213/219 (97.26%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR DQN PAAEFPRD
Sbjct: 54  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 113

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGISLPEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 114 FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 173

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 174 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIES 233

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSD+YTPQHTAVQY NVMAQLIATVQAVQS RN
Sbjct: 234 NFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of MS023672 vs. ExPASy TrEMBL
Match: A0A0A0LHQ1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=3659 GN=MED20 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.3e-112
Identity = 208/219 (94.98%), Postives = 212/219 (96.80%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR DQN PAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGISLPEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKSRQVMEEFF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIES 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSDYYT QHTAVQY NVMAQLIATVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023672 vs. ExPASy TrEMBL
Match: A0A1S3CAW6 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 GN=LOC103498750 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.3e-112
Identity = 206/219 (94.06%), Postives = 213/219 (97.26%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR DQN PAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGISLPEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIES 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSD+YTPQHTAVQY NVMAQLIATVQAVQS RN
Sbjct: 186 NFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023672 vs. ExPASy TrEMBL
Match: A0A6J1KEU1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=3661 GN=LOC111493666 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 4.9e-112
Identity = 206/219 (94.06%), Postives = 212/219 (96.80%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR D N+ A EFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DTNIAAGEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGIS+PEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRFMHIEP
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEP 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSDYYT QHTAVQYANVMAQLIATVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023672 vs. ExPASy TrEMBL
Match: A0A6J1G4U3 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=3662 GN=LOC111450720 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 4.9e-112
Identity = 206/219 (94.06%), Postives = 212/219 (96.80%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFY+PILR D N+ A EFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DTNIAAGEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGIS+PEQPNKYYLIIRGQRIVLEADFTIQ IMEKLQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVPN+SENLRGIVME+EYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRFMHIEP
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEP 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQSVRN 223
           NFAEYGLSDYYT QHTAVQYANVMAQLIATVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023672 vs. TAIR 10
Match: AT2G28230.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 323.9 bits (829), Expect = 9.8e-89
Identity = 158/215 (73.49%), Postives = 187/215 (86.98%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           VLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +Y+P+L+   N    EFPRD
Sbjct: 6   VLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQAN--QLEFPRD 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           FLGISL +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+GFQYQLGDF+L
Sbjct: 66  FLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLGDFRL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP HSEN+RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F++I+ 
Sbjct: 126 RVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFVNIDL 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQAVQ 219
           NF E+GL D YTPQHTAV+YA VMA +IATVQAV+
Sbjct: 186 NFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of MS023672 vs. TAIR 10
Match: AT4G09070.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 291.6 bits (745), Expect = 5.4e-79
Identity = 138/213 (64.79%), Postives = 176/213 (82.63%), Query Frame = 0


Query: 4   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYRPILRADQNLPAAEFPRD 63
           +LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +Y+P+L+   N   AEFPR+
Sbjct: 6   LLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQAN--QAEFPRE 65

Query: 64  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQTIMEKLQSYKLRVALNFEGFQYQLGDFQL 123
           F+GISLPE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FEG +YQLGDF+L
Sbjct: 66  FVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLGDFRL 125

Query: 124 RVGKVVPNHSENLRGIVMEMEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP H+E +RG+VME+EYLPISSM  ++++MEEF E+WQEAMSK SLPG+F++ E 
Sbjct: 126 RVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFVNKEL 185

Query: 184 NFAEYGLSDYYTPQHTAVQYANVMAQLIATVQA 217
           NF ++GL D YTPQHTAV YA  MA L+A +QA
Sbjct: 186 NFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of MS023672 vs. TAIR 10
Match: AT2G28020.1 (BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of subunit 20 of Mediator complex (TAIR:AT2G28230.1); Has 37 Blast hits to 37 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 87.0 bits (214), Expect = 2.0e-17
Identity = 44/68 (64.71%), Postives = 56/68 (82.35%), Query Frame = 0


Query: 151 MEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTPQHTAVQ-YANVMAQ 210
           ME +R+VMEEF E+W EA++K SLPG+F++ + NF E+GL D YTPQHTAV+ YA VMA 
Sbjct: 1   MEIARKVMEEFLEIWNEALAKRSLPGKFVNKDLNFGEFGLGDNYTPQHTAVRNYALVMAH 60

Query: 211 LIATVQAV 218
           +IATVQAV
Sbjct: 61  MIATVQAV 68

The following BLAST results are available for this feature:

BLAST of MS023672 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889134.1	2.8e-114	96.35	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Beninca...	[more]
XP_008459711.1	2.7e-112	94.06	PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform ...	[more]
XP_004141069.1	2.7e-112	94.98	mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60...	[more]
KAA0061469.1	2.7e-112	94.06	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis...	[more]
XP_022946745.1	1.0e-111	94.06	mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata...	[more]

BLAST of MS023672 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPF4	1.4e-87	73.49	Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana ...	[more]
Q9M0S0	7.6e-78	64.79	Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana ...	[more]
Q54FL3	3.4e-09	27.92	Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium...	[more]

BLAST of MS023672 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CH02	1.3e-112	94.06	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. maku...	[more]
A0A0A0LHQ1	1.3e-112	94.98	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=365...	[more]
A0A1S3CAW6	1.3e-112	94.06	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 G...	[more]
A0A6J1KEU1	4.9e-112	94.06	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=36...	[more]
A0A6J1G4U3	4.9e-112	94.06	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=...	[more]

BLAST of MS023672 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28230.1	9.8e-89	73.49	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT4G09070.1	5.4e-79	64.79	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT2G28020.1	2.0e-17	64.71	BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013921	Mediator complex, subunit Med20	PFAM	PF08612	Med20	coord: 4..207 e-value: 5.9E-42 score: 143.6
IPR013921	Mediator complex, subunit Med20	PANTHER	PTHR12465	UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49	coord: 4..216
None	No IPR available	PANTHER	PTHR12465:SF0	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20	coord: 4..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023672.1	MS023672.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003712	transcription coregulator activity