MS023175 (gene) Bitter gourd (TR) v1

Overview
NameMS023175
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontranscription factor Pur-alpha 1
Locationscaffold78: 682018 .. 686248 (-)
RNA-Seq ExpressionMS023175
SyntenyMS023175
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGCAATTCTGGCGGAGGGGGAGTGGGAATTGGGGGAACGACGGCGGGTGCTGTTGCGGCGGTGGGAGGAGTAGCCGGCGGCGGCGGCGGAGGGAATGACGTGGAGCTGATGTGCAAAACGCTGCAGGTGGAGCACAAGCTGTTCTACTTCGATCTGAAGGAGAATCCCAGAGGCCGATATCTGAAGATTTCGGAGAAGACATCGGCTACAAGGTCAACCATCATCGTTCCCTTCTCTGGGATTCCATGGTTCTTAGATCTCTTCAATTACTATATCAATTCTGATGATCCGGAGGTTTTTAGCAAGGAATTGCAGCTCGACACAAAGGCATCTTTGTTTCTCTATCTCTGTTTCTCTTCTTCCTCCATACTTTTTTTGTTCTCTCTTTGTTCTTGTGGTCAACTTTTTGCTTTTTATGCACTCACCGTGCCTTTCAGGTGTTTTACTTCGACATTGGCGAAAATAGGAGGGGTCGCTTCTTGAAGGTAAATTGCTTTTTCCTGCTACCCTTTTTGTTGTTTTTGTTTCTTTTCTCGAGATTCCAACTTTCTCGAATTTCCAAATTTTTTTTCTTGAAAGTACTCTCTGTAGCATGTTTGTTTGATTCTACCAAATTAGTGTTCTGGAGATTGGGATTGGTCTTGTTGGTCTGGAATTTCACTTAAAATTTGCGACAGTGCTCAGAAAAAGCTGACAGATCCGCACATCTTTTCTCCTAACTATTCTGTTTTATACTTCAAATTTTCTTTGATTGAAGTTCTACTTCTGCCCAGTTCATTTCAATTACAAGTTCTAACTTGGTAGCTTCTGATGCCCAAGACGAATATTAACTCTTATCAGAATGGGGACAAGAGAGAGAATGGAATTAATTGAACTATGAGTTTGTAATGCTGTAACATTCCATGTTATGTGTCCTTTTTTTGGTGAAGGAAAAAGAAGTCTATCTTTATTTTTCTTGATGACTTTATAGCCTTCCGTATGACAATACTTCCAAATGTGTTTTTCCAAATATGAGCAGTTTCTCAAAATTATACCATGATTAATAGAAATTCCTGAACAGTTTGATTTGACCTTTTCTTTTCTTTCTTCTTCCTCCTTTTTTCATTCACTTGTTCATCTGCCATGGGAATTAGAAGCCAAGGCTATTTGCAAAATATTTAGGCTTTATATTGAAAGCCTAAGAAACAAATCATGGATATGTTGGCACCTGCCTGTTTAAAATATTAGCACAGACATATGAATGACATGGGATATAGAAAATGTTTTTTCTATAATTTTTATCTAAATAGTACATAATGATTAAATTAATGCTAGTGTAAAATTGATTAAATTATTTATAAGTAACATAATATTTTATATAGAAATTTATATCAAAAGTGAAACTGAAATATTTACTTACAATTATATACTTACACAACAAGTTCAAGTTCACACGGACTAACAAGAGCGTAGCTCAACTAGTTAAAGCATTATAACCTCGACCAAAAGGTGAGATGATGGAATCCCCACCTCCATGTATGAACTTAAGGGAAAAAAAAAGACACAAGTCCATGCAAACTAGAAAGTTCCATTTCAAATATTGTTTACTGAATCCTCCACACTTTATCATATGGGACAACTGAAAAAGGGTTCTTCATCCATTAATTATACTACACCTTGATTATAGCTATGTCTGATTTTTTATTTTTTATTTTTTTTTAATCATTTTGTGTTATTATTTCTTTGTACATGTTTGCTTTTCCATCTTGGTTTTGTCCATGCATATACTTCCTCTATAGCCAATTTTGTACCACTAGTTACATTATAAATGATTCATAGGGTCTTATGAAGCACGGTTCATACATAATGCCTACACCTTGATTACTTGGCCTACGGTCCTATGGACAACTTATGAGGCACATTATCAGAGAGTCTGGAGAGAACTGGGTTAGAAGGCATAATTCTTGGGAGCTTTCCAACTATTAGATTTCATAAATGGAGGATGAAACCATCATTTTAACTTGCTTTGCATTCTTAATCTGATATTAGATCTATTTTGCATTTATATTTTCTTTTATGTTGTCTTTAAATTATTTTTTGTATAGAGCATATAAGTGTTTCCTGCAAATTTTATGACGCCTCATGCACGTAACTCTTTAGTTTTTGTTTTTTTGACCATTGTTCTTGGAATGAATGATGGGACTGAAGTAATGTGACTGGACACTAAAACTAAAGATTTCCTTTGCGGAAAAAAAAAAAAAAAAATAAAGAAGAAAAAGGCACTTTTGGNNNNNNNNNNNNNNNNNNTTCCTTTGCGGAAAAAAAAAAAAAAAAACTAAAGATGTCACTGGCACTTTTGGCTTTACTTCTCAAGTATATGTTTCCAACTCTGAGCTCAGACCTTGGAACTGGACGTGTTACTCAAGTTAGGCCGAGGTTTATAGCAAGTGTTGGAAGTGTTATCTTCTAAAACAGAAACAGATTTCTGGTGAAGAAAAATGCATGTTTTAGCTGTTCTCAGTGTTCATGTGATAGATCTTCTCCAGGATATTATAAAGAGAGAAAAGAAAATGCTGACGTAGAATGGCTCAACCTCTGCAGTAACCAGCTTTTTAAAAGTCCCATTTTTTTCCATCACCATCTTTGGCCAGCCATTACCACCTTCTCCATTATCAAAGAGTTGACTTACCGTGCAAAATAATCCCTACAGGCTGACCCATAGCCCCTTCATACCAGACAGCCCAGCAACCCCTCTTTTGATCTCTTTGGAGTTCCATCTTCATGTGTCCGATCATGCAAGTCAAATCTGCAATTCACTTGTTCCCAGTCTCTCATGTTCTGTCCCTTGTGCTTTTGCTTGTTGAATCTACAATAAGCTTCCACATGTGCTACGGGTCTCCAATGCTCCCTTGTTTCTACTTTAGGGTATAAATTTATTTAAAGTTTCTGTAAACATAGACGTGGTGTGTAGTTAATGAGTGTAAATTCACTTTCTGTATCTTGTATAATGTTTATTAATGGCTAAATATTGTCCACAAGATTTTAACTGATTCCATCTTTCTTTTAAAAATAATAGTATGGATCTGAACCGTTTGGTAGAATCCTTGACTACTTGTTGAGCCCATTTCAGCTCTGGTTTATTCTTACTTTGATCTGTTTTCCAACTTCAATTCTGTTACAAGTAAATACCAACTAGTGCTGAATGATTCAGATTCTGGGATTGGAGGGTAGATAATTTGACCCCTTCAGCTAGGCACAATTGAGTTACTGTTGCCTTCTTCTGAATTGTAAGGCTTAAGATTAAATTTTTGGAAATGTTGAATAATTCCATCCTCTCTTATTAGTTTGTTTTTGTAAACAAATATTGGTGTATAGACAGTTTATAACTTTTGTCCCTTCTTTCTTTTGGTTCCTCTAAAAAAGAAAAAGGACACATAATTTTTAAGTCTGGTTTATCGGGTTGCAAATAGAGATGCCTTTGGGGAATTTCAAAGCTTGTCAACTGGGCAGGTATCTGAAGCTTCTGTCAGTAGAAACCGCAGCACCATTATCGTTCCGGCCGGAAGCAACCGGGACGAGGGATGGACTGCATTCAGAAACATTTTGGCAGAGATCAATGAAGCATCTAGGCTTTTCATTCTGCCGAATCAGGTTTTGCTGTTCACAGCTAACTTCTCATCATTCTTTTTTAGGGCTAACTTCTCATCGTTTCTTGCTTAACACCATTTTGTGCGGTGTTTTCCCCGAAAACCAGGAAAATTCTGAACACTCGGAACGTATCGTCGGGCTTTCTGACGATGTAGGTGCTGGCTTCATATCAGGACATAGTAGTCGATCCGGGCCAAACTCTGACTTGAACGTTGACAGACAAGTAGACTTGTCAGCTCAAGATGACATGGGCAATCTGGGGGTGTCAAAAGTGATCAGAGCTGATCAGAAGAGATTCTTTTTCGATTTGGGAAGTAACAACCGAGGTCATTTCTTGAGGATATCTGAGGTAATGATCAAGAAAACTGAGTTTAATCTGTTAAAGTCAGTCTTGAACCGTTTTTTCAGTTTCTTGGTATGAACTTCATCAAACCATTATCGATTACAGGTTGCAGGGGCAGATCGTTCTTCGATCATTCTCCCATTGTCGGGGCTAAAGCAGTTCTACGAAATAGTGGGACATTTCGTGGAGATCACCAAGGATAGAATTGAAGGTATGACAGGCGTGAACGTTAGGACCGTGGATCCGCCTCAGAGA

mRNA sequence

ATGGAGGGCAATTCTGGCGGAGGGGGAGTGGGAATTGGGGGAACGACGGCGGGTGCTGTTGCGGCGGTGGGAGGAGTAGCCGGCGGCGGCGGCGGAGGGAATGACGTGGAGCTGATGTGCAAAACGCTGCAGGTGGAGCACAAGCTGTTCTACTTCGATCTGAAGGAGAATCCCAGAGGCCGATATCTGAAGATTTCGGAGAAGACATCGGCTACAAGGTCAACCATCATCGTTCCCTTCTCTGGGATTCCATGGTTCTTAGATCTCTTCAATTACTATATCAATTCTGATGATCCGGAGGTTTTTAGCAAGGAATTGCAGCTCGACACAAAGGCATCTTTAGATGCCTTTGGGGAATTTCAAAGCTTGTCAACTGGGCAGGTATCTGAAGCTTCTGTCAGTAGAAACCGCAGCACCATTATCGTTCCGGCCGGAAGCAACCGGGACGAGGGATGGACTGCATTCAGAAACATTTTGGCAGAGATCAATGAAGCATCTAGGCTTTTCATTCTGCCGAATCAGGAAAATTCTGAACACTCGGAACGTATCGTCGGGCTTTCTGACGATGTAGGTGCTGGCTTCATATCAGGACATAGTAGTCGATCCGGGCCAAACTCTGACTTGAACGTTGACAGACAAGTAGACTTGTCAGCTCAAGATGACATGGGCAATCTGGGGGTGTCAAAAGTGATCAGAGCTGATCAGAAGAGATTCTTTTTCGATTTGGGAAGTAACAACCGAGGTCATTTCTTGAGGATATCTGAGGTTGCAGGGGCAGATCGTTCTTCGATCATTCTCCCATTGTCGGGGCTAAAGCAGTTCTACGAAATAGTGGGACATTTCGTGGAGATCACCAAGGATAGAATTGAAGGTATGACAGGCGTGAACGTTAGGACCGTGGATCCGCCTCAGAGA

Coding sequence (CDS)

ATGGAGGGCAATTCTGGCGGAGGGGGAGTGGGAATTGGGGGAACGACGGCGGGTGCTGTTGCGGCGGTGGGAGGAGTAGCCGGCGGCGGCGGCGGAGGGAATGACGTGGAGCTGATGTGCAAAACGCTGCAGGTGGAGCACAAGCTGTTCTACTTCGATCTGAAGGAGAATCCCAGAGGCCGATATCTGAAGATTTCGGAGAAGACATCGGCTACAAGGTCAACCATCATCGTTCCCTTCTCTGGGATTCCATGGTTCTTAGATCTCTTCAATTACTATATCAATTCTGATGATCCGGAGGTTTTTAGCAAGGAATTGCAGCTCGACACAAAGGCATCTTTAGATGCCTTTGGGGAATTTCAAAGCTTGTCAACTGGGCAGGTATCTGAAGCTTCTGTCAGTAGAAACCGCAGCACCATTATCGTTCCGGCCGGAAGCAACCGGGACGAGGGATGGACTGCATTCAGAAACATTTTGGCAGAGATCAATGAAGCATCTAGGCTTTTCATTCTGCCGAATCAGGAAAATTCTGAACACTCGGAACGTATCGTCGGGCTTTCTGACGATGTAGGTGCTGGCTTCATATCAGGACATAGTAGTCGATCCGGGCCAAACTCTGACTTGAACGTTGACAGACAAGTAGACTTGTCAGCTCAAGATGACATGGGCAATCTGGGGGTGTCAAAAGTGATCAGAGCTGATCAGAAGAGATTCTTTTTCGATTTGGGAAGTAACAACCGAGGTCATTTCTTGAGGATATCTGAGGTTGCAGGGGCAGATCGTTCTTCGATCATTCTCCCATTGTCGGGGCTAAAGCAGTTCTACGAAATAGTGGGACATTTCGTGGAGATCACCAAGGATAGAATTGAAGGTATGACAGGCGTGAACGTTAGGACCGTGGATCCGCCTCAGAGA

Protein sequence

MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEFQSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHSERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDPPQR
Homology
BLAST of MS023175 vs. NCBI nr
Match: XP_022147650.1 (transcription factor Pur-alpha 1 [Momordica charantia])

HSP 1 Score: 558.1 bits (1437), Expect = 4.6e-155
Identity = 290/305 (95.08%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180
           +     +VSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240
           ERIVGLSDDVGAGFISGHSS+SGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF
Sbjct: 181 ERIVGLSDDVGAGFISGHSSQSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 306
           DPPQR
Sbjct: 301 DPPQR 305

BLAST of MS023175 vs. NCBI nr
Match: XP_011660046.1 (transcription factor Pur-alpha 1 [Cucumis sativus] >XP_011660047.1 transcription factor Pur-alpha 1 [Cucumis sativus] >XP_031736036.1 transcription factor Pur-alpha 1 [Cucumis sativus] >KGN66342.1 hypothetical protein Csa_007516 [Cucumis sativus])

HSP 1 Score: 535.8 bits (1379), Expect = 2.4e-148
Identity = 280/305 (91.80%), Postives = 286/305 (93.77%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGNSGGGGVGIGGTTAG VAA GG   GGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVGIGGTTAGGVAAGGG--AGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180
           +     +VSEASVSRNRSTIIVPAGSNRDEGW+AFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240
           ER+ GLSDDVGAGFISGHSS+SGP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKRFFF
Sbjct: 181 ERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 306
           DPPQR
Sbjct: 301 DPPQR 303

BLAST of MS023175 vs. NCBI nr
Match: XP_023531642.1 (transcription factor Pur-alpha 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.4 bits (1378), Expect = 3.2e-148
Identity = 278/305 (91.15%), Postives = 284/305 (93.11%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGNSGGGGV IGGTTAG+VA  G   GGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180
           +     +VSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240
           ER+VGLSDDVGAGFISGHSS+ GP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKRFFF
Sbjct: 181 ERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 306
           DPP R
Sbjct: 301 DPPHR 305

BLAST of MS023175 vs. NCBI nr
Match: XP_022929520.1 (transcription factor Pur-alpha 1-like [Cucurbita moschata])

HSP 1 Score: 533.5 bits (1373), Expect = 1.2e-147
Identity = 277/305 (90.82%), Postives = 284/305 (93.11%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEG+SGGGGV IGGTTAG+VA  G   GGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180
           +     +VSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240
           ER+VGLSDDVGAGFISGHSS+ GP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKRFFF
Sbjct: 181 ERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 306
           DPP R
Sbjct: 301 DPPHR 305

BLAST of MS023175 vs. NCBI nr
Match: XP_022967890.1 (transcription factor Pur-alpha 1-like [Cucurbita maxima])

HSP 1 Score: 533.1 bits (1372), Expect = 1.6e-147
Identity = 281/308 (91.23%), Postives = 287/308 (93.18%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGV---AGGGGGGNDVELMCKTLQVEHKLFYFDLKEN 60
           MEGNSGGGGV IGGTTAG+V AVGGV    GGGGGGNDVELMCKTLQVEHKLFYFDLKEN
Sbjct: 1   MEGNSGGGGVSIGGTTAGSV-AVGGVTGGGGGGGGGNDVELMCKTLQVEHKLFYFDLKEN 60

Query: 61  PRGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAF 120
           PRGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      
Sbjct: 61  PRGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDI 120

Query: 121 GEFQSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENS 180
           GE +     +VSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENS
Sbjct: 121 GENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENS 180

Query: 181 EHSERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKR 240
           EHSER+VGLSDDVGAGFISGHSS+ GP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKR
Sbjct: 181 EHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKR 240

Query: 241 FFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNV 300
           FFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNV
Sbjct: 241 FFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNV 300

Query: 301 RTVDPPQR 306
           RTVDPP R
Sbjct: 301 RTVDPPHR 307

BLAST of MS023175 vs. ExPASy Swiss-Prot
Match: Q9SKZ1 (Transcription factor Pur-alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PURA1 PE=1 SV=2)

HSP 1 Score: 412.5 bits (1059), Expect = 4.1e-114
Identity = 223/306 (72.88%), Postives = 251/306 (82.03%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           ME NSGGGG   GG      A  GG  GGGGGG+DVEL+ KTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEANSGGGGGAEGGR-----AVTGG--GGGGGGSDVELVSKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVP SGI WFLDLFNYY+NS++ E+FSKELQLD+K      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKELQLDSKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQ-ENSEH 180
           +     +VSEASVSRNRSTIIVPAGS+ DEGW AFRNILAEI+EAS LF++PNQ + S+ 
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEASGLFVMPNQVKPSDG 180

Query: 181 SERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFF 240
            E +V   DDVGAGFI GH S+   +S+ NVDR +D   Q++ G  GVSKVIRADQKRFF
Sbjct: 181 QEHLV---DDVGAGFIPGHGSQQPSSSEHNVDRTIDSPGQEETGMTGVSKVIRADQKRFF 240

Query: 241 FDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRT 300
           FDLG+NNRGHFLRISEVAG+DRSSIILPLSGLKQF+E++GHFVEITKD+IEGMTG NVRT
Sbjct: 241 FDLGNNNRGHFLRISEVAGSDRSSIILPLSGLKQFHEVIGHFVEITKDKIEGMTGANVRT 296

Query: 301 VDPPQR 306
           VDPPQR
Sbjct: 301 VDPPQR 296

BLAST of MS023175 vs. ExPASy Swiss-Prot
Match: Q8AVS4 (Transcriptional activator protein Pur-beta-B OS=Xenopus laevis OX=8355 GN=purb-b PE=2 SV=3)

HSP 1 Score: 70.1 bits (170), Expect = 4.9e-11
Identity = 75/275 (27.27%), Postives = 108/275 (39.27%), Query Frame = 0

Query: 24  GGVAGGGGGGND--------VELMCKTLQVEHKLFYFDLKENPRGRYLKISE-KTSATRS 83
           GG +GG  G +          EL  K L +++K FY D+K+N +GR++KI+E     ++S
Sbjct: 11  GGSSGGPSGFSQHMSREQETQELASKRLDIQNKRFYLDVKQNAKGRFIKIAEVGAGGSKS 70

Query: 84  TIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEFQSLSTGQVSEASVS 143
            + +  +    F D    +I        S   Q+   +  D  G          SE  V 
Sbjct: 71  RLTLSMAVAAEFRDYLGDFIEHYAQLGPSSPEQIAQASGEDGAGGPGGPRRALKSEFLVR 130

Query: 144 RNRSTIIVPAGSNRDEGWTAFRNILAEIN---------------EASRLFILPNQENSEH 203
            NR   +    + R      F  I   IN               ++ +   LP Q   E 
Sbjct: 131 ENRKYYLDLKENQRGR----FLRIRQTINRGPGFSGGTGGGPGLQSGQTIALPAQGLIEF 190

Query: 204 SERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFF 263
            + +  L DD G     G    SG +                 G L     I  D KRFF
Sbjct: 191 RDALAKLIDDYGGEDDEGMGLGSGASGG-------GAGGGGMYGELPEGTSITVDSKRFF 250

Query: 264 FDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQF 275
           FD+GSN  G FLR+SEV  + R+SI +PL    +F
Sbjct: 251 FDVGSNKYGVFLRVSEVKPSYRNSITVPLKAWGKF 274

BLAST of MS023175 vs. ExPASy Swiss-Prot
Match: O35295 (Transcriptional activator protein Pur-beta OS=Mus musculus OX=10090 GN=Purb PE=1 SV=3)

HSP 1 Score: 69.3 bits (168), Expect = 8.4e-11
Identity = 85/294 (28.91%), Postives = 117/294 (39.80%), Query Frame = 0

Query: 3   GNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 62
           G  GGGG G GG         GG  G GG     EL  K L +++K FY D+K+N +GR+
Sbjct: 11  GGGGGGGGGPGGFQPAPRGGGGGGGGPGGEQETQELASKRLDIQNKRFYLDVKQNAKGRF 70

Query: 63  LKISE-KTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEFQ 122
           LKI+E     ++S + +  +    F D    +I        S   QL   A  +  G  +
Sbjct: 71  LKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAE-EGGGPRR 130

Query: 123 SLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEIN---------------EAS 182
           +L     SE  V  NR   +    + R      F  I   +N               ++ 
Sbjct: 131 ALK----SEFLVRENRKYYLDLKENQRGR----FLRIRQTVNRGGGGFGGGPGPGGLQSG 190

Query: 183 RLFILPNQENSEHSERIVGLSDDVG------AGFISGHSSRSGPNSDLNVDRQVDLSAQD 242
           +   LP Q   E  + +  L DD G      AG   G     GP   L            
Sbjct: 191 QTIALPAQGLIEFRDALAKLIDDYGGDEDELAGGPGG--GAGGPGGGL------------ 250

Query: 243 DMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQF 275
             G L     I  D KRFFFD+G N  G FLR+SEV  + R++I +P     +F
Sbjct: 251 -YGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKF 280

BLAST of MS023175 vs. ExPASy Swiss-Prot
Match: Q6PAC9 (Transcriptional activator protein Pur-beta-A OS=Xenopus laevis OX=8355 GN=purb-a PE=2 SV=3)

HSP 1 Score: 68.9 bits (167), Expect = 1.1e-10
Identity = 75/276 (27.17%), Postives = 110/276 (39.86%), Query Frame = 0

Query: 24  GGVAGGGGGGND--------VELMCKTLQVEHKLFYFDLKENPRGRYLKISE-KTSATRS 83
           GG +GG GG +          EL  K L +++K FY D+K+N +GR++KI+E     ++S
Sbjct: 11  GGSSGGPGGFSQHMSREQETQELATKRLDIQNKRFYLDVKQNAKGRFIKIAEVGAGGSKS 70

Query: 84  TIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEFQSLSTGQVSEASVS 143
            + +       F D    +I        S   Q+   +  D  G          SE  V 
Sbjct: 71  RLTLSMGVAAEFRDYLGDFIEHYAQLGPSSPEQIAQASGEDGAGGPGGPRRALKSEFLVR 130

Query: 144 RNRSTII------------VPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHSER 203
            NR   +            +    NR  G++      A + ++ +   LP Q   E  + 
Sbjct: 131 ENRKYYLDLKENQRGRFLRIRQTINRGPGFSGGAGGGAGL-QSGQTIALPAQGLIEFRDA 190

Query: 204 IVGLSDDVGA----GFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRF 263
           +  L DD G     G   G +   G                   G L     I  D KRF
Sbjct: 191 LAKLIDDYGGEDDEGVGLGGAGGGGGGG--------------MYGELPEGTSITVDSKRF 250

Query: 264 FFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQF 275
           FFD+GSN  G FLR+SEV  + R+SI +PL    +F
Sbjct: 251 FFDVGSNKYGVFLRVSEVKPSYRNSITVPLKAWGKF 271

BLAST of MS023175 vs. ExPASy Swiss-Prot
Match: Q00577 (Transcriptional activator protein Pur-alpha OS=Homo sapiens OX=9606 GN=PURA PE=1 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 9.3e-10
Identity = 78/278 (28.06%), Postives = 106/278 (38.13%), Query Frame = 0

Query: 5   SGGGGVGIGGTTAGAVAAVGGVAGGGGGG-------NDVELMCKTLQVEHKLFYFDLKEN 64
           SGGGG G GG         GG +GGGGGG          EL  K + +++K FY D+K+N
Sbjct: 30  SGGGGGGGGG---------GGGSGGGGGGAPGGLQHETQELASKRVDIQNKRFYLDVKQN 89

Query: 65  PRGRYLKISE-KTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDA 124
            +GR+LKI+E      +S + +  S    F D    +I            QL      D 
Sbjct: 90  AKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFI--------EHYAQLGPSQPPDL 149

Query: 125 FGEFQSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL-------F 184
                       SE  V  NR   +    + R      F  I   +N    L        
Sbjct: 150 AQAQDEPRRALKSEFLVRENRKYYMDLKENQRGR----FLRIRQTVNRGPGLGSTQGQTI 209

Query: 185 ILPNQENSEHSERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSK 244
            LP Q   E  + +  L DD G                           +++   L    
Sbjct: 210 ALPAQGLIEFRDALAKLIDDYG--------------------------VEEEPAELPEGT 260

Query: 245 VIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILP 268
            +  D KRFFFD+GSN  G F+R+SEV    R+SI +P
Sbjct: 270 SLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVP 260

BLAST of MS023175 vs. ExPASy TrEMBL
Match: A0A6J1D0R2 (transcription factor Pur-alpha 1 OS=Momordica charantia OX=3673 GN=LOC111016521 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.2e-155
Identity = 290/305 (95.08%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180
           +     +VSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240
           ERIVGLSDDVGAGFISGHSS+SGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF
Sbjct: 181 ERIVGLSDDVGAGFISGHSSQSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 306
           DPPQR
Sbjct: 301 DPPQR 305

BLAST of MS023175 vs. ExPASy TrEMBL
Match: A0A0A0LZP1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G598870 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.2e-148
Identity = 280/305 (91.80%), Postives = 286/305 (93.77%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGNSGGGGVGIGGTTAG VAA GG   GGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVGIGGTTAGGVAAGGG--AGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180
           +     +VSEASVSRNRSTIIVPAGSNRDEGW+AFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240
           ER+ GLSDDVGAGFISGHSS+SGP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKRFFF
Sbjct: 181 ERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 306
           DPPQR
Sbjct: 301 DPPQR 303

BLAST of MS023175 vs. ExPASy TrEMBL
Match: A0A6J1EUL9 (transcription factor Pur-alpha 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436059 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.8e-148
Identity = 277/305 (90.82%), Postives = 284/305 (93.11%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEG+SGGGGV IGGTTAG+VA  G   GGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180
           +     +VSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240
           ER+VGLSDDVGAGFISGHSS+ GP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKRFFF
Sbjct: 181 ERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 306
           DPP R
Sbjct: 301 DPPHR 305

BLAST of MS023175 vs. ExPASy TrEMBL
Match: A0A6J1HVP1 (transcription factor Pur-alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467262 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 7.6e-148
Identity = 281/308 (91.23%), Postives = 287/308 (93.18%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGV---AGGGGGGNDVELMCKTLQVEHKLFYFDLKEN 60
           MEGNSGGGGV IGGTTAG+V AVGGV    GGGGGGNDVELMCKTLQVEHKLFYFDLKEN
Sbjct: 1   MEGNSGGGGVSIGGTTAGSV-AVGGVTGGGGGGGGGNDVELMCKTLQVEHKLFYFDLKEN 60

Query: 61  PRGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAF 120
           PRGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      
Sbjct: 61  PRGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDI 120

Query: 121 GEFQSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENS 180
           GE +     +VSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENS
Sbjct: 121 GENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENS 180

Query: 181 EHSERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKR 240
           EHSER+VGLSDDVGAGFISGHSS+ GP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKR
Sbjct: 181 EHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKR 240

Query: 241 FFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNV 300
           FFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNV
Sbjct: 241 FFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNV 300

Query: 301 RTVDPPQR 306
           RTVDPP R
Sbjct: 301 RTVDPPHR 307

BLAST of MS023175 vs. ExPASy TrEMBL
Match: A0A1S3BPW8 (transcription factor Pur-alpha 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492403 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.3e-147
Identity = 278/305 (91.15%), Postives = 285/305 (93.44%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGNSGGGGVGIGGTTAG VAA GG   G GGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVGIGGTTAGGVAAGGG--AGSGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180
           +     +VSEASVSRNRSTIIVPAGSNRDEGW+AFRNILA+INEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILADINEASRLFILPNQENSEHS 180

Query: 181 ERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240
           ER+ GLSDDVGAGFISGHSS+SGP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKRFFF
Sbjct: 181 ERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 306
           DPPQR
Sbjct: 301 DPPQR 303

BLAST of MS023175 vs. TAIR 10
Match: AT2G32080.2 (purin-rich alpha 1 )

HSP 1 Score: 417.2 bits (1071), Expect = 1.2e-116
Identity = 223/305 (73.11%), Postives = 251/305 (82.30%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           ME NSGGGG   GG      A  GG  GGGGGG+DVEL+ KTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEANSGGGGGAEGGR-----AVTGG--GGGGGGSDVELVSKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVP SGI WFLDLFNYY+NS++ E+FSKELQLD+K      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKELQLDSKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180
           +     +VSEASVSRNRSTIIVPAGS+ DEGW AFRNILAEI+EAS LF++PNQ+ S+  
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEASGLFVMPNQKPSDGQ 180

Query: 181 ERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240
           E +V   DDVGAGFI GH S+   +S+ NVDR +D   Q++ G  GVSKVIRADQKRFFF
Sbjct: 181 EHLV---DDVGAGFIPGHGSQQPSSSEHNVDRTIDSPGQEETGMTGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLG+NNRGHFLRISEVAG+DRSSIILPLSGLKQF+E++GHFVEITKD+IEGMTG NVRTV
Sbjct: 241 DLGNNNRGHFLRISEVAGSDRSSIILPLSGLKQFHEVIGHFVEITKDKIEGMTGANVRTV 295

Query: 301 DPPQR 306
           DPPQR
Sbjct: 301 DPPQR 295

BLAST of MS023175 vs. TAIR 10
Match: AT2G32080.1 (purin-rich alpha 1 )

HSP 1 Score: 412.5 bits (1059), Expect = 2.9e-115
Identity = 223/306 (72.88%), Postives = 251/306 (82.03%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           ME NSGGGG   GG      A  GG  GGGGGG+DVEL+ KTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEANSGGGGGAEGGR-----AVTGG--GGGGGGSDVELVSKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLDAFGEF 120
           RYLKISEKTSATRSTIIVP SGI WFLDLFNYY+NS++ E+FSKELQLD+K      GE 
Sbjct: 61  RYLKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKELQLDSKVFYFDIGEN 120

Query: 121 QSLSTGQVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQ-ENSEH 180
           +     +VSEASVSRNRSTIIVPAGS+ DEGW AFRNILAEI+EAS LF++PNQ + S+ 
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEASGLFVMPNQVKPSDG 180

Query: 181 SERIVGLSDDVGAGFISGHSSRSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFF 240
            E +V   DDVGAGFI GH S+   +S+ NVDR +D   Q++ G  GVSKVIRADQKRFF
Sbjct: 181 QEHLV---DDVGAGFIPGHGSQQPSSSEHNVDRTIDSPGQEETGMTGVSKVIRADQKRFF 240

Query: 241 FDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRT 300
           FDLG+NNRGHFLRISEVAG+DRSSIILPLSGLKQF+E++GHFVEITKD+IEGMTG NVRT
Sbjct: 241 FDLGNNNRGHFLRISEVAGSDRSSIILPLSGLKQFHEVIGHFVEITKDKIEGMTGANVRT 296

Query: 301 VDPPQR 306
           VDPPQR
Sbjct: 301 VDPPQR 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147650.14.6e-15595.08transcription factor Pur-alpha 1 [Momordica charantia][more]
XP_011660046.12.4e-14891.80transcription factor Pur-alpha 1 [Cucumis sativus] >XP_011660047.1 transcription... [more]
XP_023531642.13.2e-14891.15transcription factor Pur-alpha 1-like [Cucurbita pepo subsp. pepo][more]
XP_022929520.11.2e-14790.82transcription factor Pur-alpha 1-like [Cucurbita moschata][more]
XP_022967890.11.6e-14791.23transcription factor Pur-alpha 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SKZ14.1e-11472.88Transcription factor Pur-alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PURA1 PE=1 S... [more]
Q8AVS44.9e-1127.27Transcriptional activator protein Pur-beta-B OS=Xenopus laevis OX=8355 GN=purb-b... [more]
O352958.4e-1128.91Transcriptional activator protein Pur-beta OS=Mus musculus OX=10090 GN=Purb PE=1... [more]
Q6PAC91.1e-1027.17Transcriptional activator protein Pur-beta-A OS=Xenopus laevis OX=8355 GN=purb-a... [more]
Q005779.3e-1028.06Transcriptional activator protein Pur-alpha OS=Homo sapiens OX=9606 GN=PURA PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1D0R22.2e-15595.08transcription factor Pur-alpha 1 OS=Momordica charantia OX=3673 GN=LOC111016521 ... [more]
A0A0A0LZP11.2e-14891.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G598870 PE=3 SV=1[more]
A0A6J1EUL95.8e-14890.82transcription factor Pur-alpha 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436... [more]
A0A6J1HVP17.6e-14891.23transcription factor Pur-alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC11146726... [more]
A0A1S3BPW81.3e-14791.15transcription factor Pur-alpha 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492... [more]
Match NameE-valueIdentityDescription
AT2G32080.21.2e-11673.11purin-rich alpha 1 [more]
AT2G32080.12.9e-11572.88purin-rich alpha 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006628Purine-rich element binding protein familySMARTSM00712purcoord: 100..167
e-value: 0.016
score: 13.3
coord: 225..286
e-value: 4.4E-24
score: 96.0
coord: 37..98
e-value: 9.7E-23
score: 91.5
IPR006628Purine-rich element binding protein familyPFAMPF04845PurAcoord: 41..95
e-value: 7.3E-9
score: 35.3
coord: 219..281
e-value: 8.0E-13
score: 48.2
IPR006628Purine-rich element binding protein familyPANTHERPTHR12611PUR-TRANSCRIPTIONAL ACTIVATORcoord: 22..292
NoneNo IPR availableGENE3D3.10.450.700coord: 216..289
e-value: 2.7E-19
score: 70.5
coord: 99..167
e-value: 3.2E-5
score: 25.4
coord: 30..98
e-value: 1.9E-18
score: 67.8
NoneNo IPR availablePANTHERPTHR12611:SF0PURINE-RICH BINDING PROTEIN-ALPHA, ISOFORM Bcoord: 22..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023175.1MS023175.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0032422 purine-rich negative regulatory element binding
molecular_function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding