MS023139 (gene) Bitter gourd (TR) v1

BLAST of MS023139 vs. NCBI nr
Match: XP_022987083.1 (subtilisin-like protease SBT1.8 [Cucurbita maxima])

HSP 1 Score: 1423.7 bits (3684), Expect = 0.0e+00
Identity = 707/738 (95.80%), Postives = 723/738 (97.97%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           KRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS TSDSLLYTYT+AYHGFAASLDP+EA
Sbjct: 27  KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSATSDSLLYTYTSAYHGFAASLDPDEA 86

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT
Sbjct: 87  ELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 146

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 147 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 206

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           K REN+SPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 207 KSRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 266

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 267 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 326

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPV LVY+KGSN
Sbjct: 327 KASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVALVYNKGSN 386

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           +SSNMCLPGSLEPA VRGKVVICDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 387 SSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 446

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 447 SHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 506

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 507 ILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 566

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
            WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNPWAHGAGHVDPHKALSPGLLYDIST
Sbjct: 567 SWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFSNPWAHGAGHVDPHKALSPGLLYDIST 626

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYI FLCSLDYGIDHVQAIVKR NITCS+KFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 627 NDYITFLCSLDYGIDHVQAIVKRSNITCSKKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 686

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVYEVATTAPSVVKV VKP+KL F+KVGERKRYTVTFVAS +AA+TTR+GFGSI
Sbjct: 687 NVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGERKRYTVTFVASRDAARTTRYGFGSI 746

Query: 721 AWSNDQHQVRSPVAFAWT 739
           AWSNDQHQVRSPVAFAWT
Sbjct: 747 AWSNDQHQVRSPVAFAWT 764

BLAST of MS023139 vs. NCBI nr
Match: XP_023516159.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 706/738 (95.66%), Postives = 723/738 (97.97%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           KRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS TSDSLLYTYT+AYHGFAASLDP+EA
Sbjct: 26  KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSATSDSLLYTYTSAYHGFAASLDPDEA 85

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT
Sbjct: 86  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 145

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 146 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 205

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           K REN+SPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 206 KSRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 265

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 266 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 325

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPV LVY+KGSN
Sbjct: 326 KASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVALVYNKGSN 385

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           +SSNMCLPGSLEPA VRGKVVICDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 386 SSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 445

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 446 SHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 505

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 506 ILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 565

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
            WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNPWAHGAGHVDPHKALSPGL+YDIST
Sbjct: 566 SWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFSNPWAHGAGHVDPHKALSPGLVYDIST 625

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYI FLCSLDYGIDHVQAI KR NITCS+KFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 626 NDYITFLCSLDYGIDHVQAIAKRSNITCSKKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 685

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVYEVATTAPSVVKV VKP+KL F+KVGERKRYTVTFVAS +AA+TTR+GFGSI
Sbjct: 686 NVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGERKRYTVTFVASRDAARTTRYGFGSI 745

Query: 721 AWSNDQHQVRSPVAFAWT 739
           AWSNDQHQVRSPVAFAWT
Sbjct: 746 AWSNDQHQVRSPVAFAWT 763

BLAST of MS023139 vs. NCBI nr
Match: KAG6590040.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 706/738 (95.66%), Postives = 723/738 (97.97%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           KRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS TSDSLLYTYT+AYHGFAASLDP+EA
Sbjct: 26  KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSATSDSLLYTYTSAYHGFAASLDPDEA 85

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT
Sbjct: 86  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 145

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 146 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 205

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           K REN+SPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 206 KSRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 265

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 266 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 325

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPV LVY+KGSN
Sbjct: 326 KASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVALVYNKGSN 385

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           +SSNMCLPGSLEPA VRGKVVICDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 386 SSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 445

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 446 SHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 505

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 506 ILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 565

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
            WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGL+YDIST
Sbjct: 566 SWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLVYDIST 625

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYI FLCSLDYGIDHVQAI KR NITC +KFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 626 NDYITFLCSLDYGIDHVQAIAKRSNITCLKKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 685

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVYEVATTAPSVVKV VKP+KL F+KVGERKRYTVTFVAS +AA+TTR+GFGSI
Sbjct: 686 NVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGERKRYTVTFVASRDAARTTRYGFGSI 745

Query: 721 AWSNDQHQVRSPVAFAWT 739
           AWSNDQHQVRSPVAFAWT
Sbjct: 746 AWSNDQHQVRSPVAFAWT 763

BLAST of MS023139 vs. NCBI nr
Match: XP_022960792.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-like protease SBT1.8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 706/738 (95.66%), Postives = 723/738 (97.97%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           KRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS TSDSLLYTYT+AYHGFAASLDP+EA
Sbjct: 27  KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSATSDSLLYTYTSAYHGFAASLDPDEA 86

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT
Sbjct: 87  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 146

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 147 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 206

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           K REN+SPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 207 KSRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 266

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 267 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 326

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPV LVY+KGSN
Sbjct: 327 KASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVALVYNKGSN 386

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           +SSNMCLPGSLEPA VRGKVVICDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 387 SSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 446

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 447 SHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 506

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 507 ILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 566

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
            WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGL+YDIST
Sbjct: 567 SWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLVYDIST 626

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYI FLCSLDYGIDHVQAI KR NITC +KFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 627 NDYITFLCSLDYGIDHVQAIAKRSNITCLKKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 686

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVYEVATTAPSVVKV VKP+KL F+KVGERKRYTVTFVAS +AA+TTR+GFGSI
Sbjct: 687 NVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGERKRYTVTFVASRDAARTTRYGFGSI 746

Query: 721 AWSNDQHQVRSPVAFAWT 739
           AWSNDQHQVRSPVAFAWT
Sbjct: 747 AWSNDQHQVRSPVAFAWT 764

BLAST of MS023139 vs. NCBI nr
Match: XP_038879224.1 (subtilisin-like protease SBT1.8 [Benincasa hispida])

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 701/738 (94.99%), Postives = 723/738 (97.97%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           K+TYIVRMKH+ALPS+YLTHHDWYSA+LQSLSSS+TSDSLLYTYT+AYHGFAASLD  EA
Sbjct: 24  KKTYIVRMKHNALPSEYLTHHDWYSAHLQSLSSSSTSDSLLYTYTSAYHGFAASLDSNEA 83

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYED+VY LHTTRTPGFLGLDSDFGLWEGHNTQDL+QASHDVIIGVLDT
Sbjct: 84  ELLRQSDSVLGVYEDSVYTLHTTRTPGFLGLDSDFGLWEGHNTQDLNQASHDVIIGVLDT 143

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRGECESG DFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 144 GIWPESKSFDDTGMPEIPSRWRGECESGADFSPSLCNKKLIGARSFSKGYQMASGGGYFR 203

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           KPREN+SPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 204 KPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 263

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 264 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 323

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQ+GNGKRFTGVSLYSGQGMG+K VELVY+KGSN
Sbjct: 324 KASLANVAPWIMTVGAGTLDRDFPAYVQIGNGKRFTGVSLYSGQGMGSKAVELVYNKGSN 383

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           TSSNMCLPGSLEPAVVRGKVV+CDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 384 TSSNMCLPGSLEPAVVRGKVVVCDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 443

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVGRKTGDLIRQYV+S + PTAVLSFGGT+LNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 444 SHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQ 503

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 504 ILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 563

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
           QWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNPWAHGAGHVDPHKALSPGLLYDIST
Sbjct: 564 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGHVDPHKALSPGLLYDIST 623

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYIAFLCSLDYGIDHVQAIVKR NITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 624 NDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 683

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVYEVATTAPSVVKV VKP+KL FTKVGERKRYTVTFVAS +AAQT RFGFGSI
Sbjct: 684 NVGAAGSVYEVATTAPSVVKVTVKPSKLVFTKVGERKRYTVTFVASRDAAQTMRFGFGSI 743

Query: 721 AWSNDQHQVRSPVAFAWT 739
           AW+N QHQVRSPVAFAWT
Sbjct: 744 AWNNYQHQVRSPVAFAWT 761

BLAST of MS023139 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 543/738 (73.58%), Postives = 629/738 (85.23%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           K+TYI+R+ H   P  +LTHHDWY++ L S S      SLLYTYT ++HGF+A LD  EA
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 61  E-LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLD 120
           + LL  S+S+L ++ED +Y LHTTRTP FLGL+S+FG+       DL  +S+ VIIGVLD
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------HDLGSSSNGVIIGVLD 146

Query: 121 TGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYF 180
           TG+WPES+SFDDT MPEIPS+W+GECESG DF   LCNKKLIGARSFSKG+QMASGGG+ 
Sbjct: 147 TGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFS 206

Query: 181 RKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTG 240
            K RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G ARGMA +ARVA YK CW TG
Sbjct: 207 SK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTG 266

Query: 241 CFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGP 300
           CFGSDILA MDRAI DGVDVLSLSLGGGSAPYYRDTIAIGAF+AME+GVFVSCSAGNSGP
Sbjct: 267 CFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGP 326

Query: 301 NRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGS 360
            RAS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG GMG KP+ELVY+KG+
Sbjct: 327 TRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGN 386

Query: 361 NTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVA 420
           ++SSN+CLPGSL+ ++VRGK+V+CDRG+NARVEKG VVRDAGG+GMI+ANTA+SGEELVA
Sbjct: 387 SSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVA 446

Query: 421 DSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTP 480
           DSHLLPA+AVG+KTGDL+R+YVKS +KPTA+L F GTVL+V+PSPVVAAFSSRGPN VTP
Sbjct: 447 DSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTP 506

Query: 481 QILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAH 540
           +ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSGTSMSCPHISGLA LLKAAH
Sbjct: 507 EILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAH 566

Query: 541 PQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIS 600
           P+WSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GHVDP KALSPGL+YDIS
Sbjct: 567 PEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDIS 626

Query: 601 TEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIV 660
           TE+YI FLCSLDY +DH+ AIVKRP++ CS+KF+DPGQLNYPSFSV+FG KRVVRYTR V
Sbjct: 627 TEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREV 686

Query: 661 TNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGS 720
           TNVGAA SVY+V       V + VKP+KL+F  VGE+KRYTVTFV+    + T +  FGS
Sbjct: 687 TNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGS 746

Query: 721 IAWSNDQHQVRSPVAFAW 738
           I WSN QH+VRSPVAF+W
Sbjct: 747 ITWSNPQHEVRSPVAFSW 751

BLAST of MS023139 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 2.1e-233
Identity = 422/742 (56.87%), Postives = 533/742 (71.83%), Query Frame = 0

Query: 3   TYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEAEL 62
           TYIV M    +PS +  H +WY ++L+S+S S     LLYTY  A HGF+  L  EEA+ 
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA---ELLYTYENAIHGFSTRLTQEEADS 90

Query: 63  LRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDL--DQASH-DVIIGVLD 122
           L     V+ V  +  Y LHTTRTP FLGLD         +T DL  +  S+ DV++GVLD
Sbjct: 91  LMTQPGVISVLPEHRYELHTTRTPLFLGLD--------EHTADLFPEAGSYSDVVVGVLD 150

Query: 123 TGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYF 182
           TG+WPESKS+ D G   IPS W+G CE+G +F+ SLCN+KLIGAR F++GY+  S  G  
Sbjct: 151 TGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE--STMGPI 210

Query: 183 RKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTG 242
            + +E++SPRD DGHGTHT+STAAGS V  ASLLGYA G ARGMAP+ARVA YK CW  G
Sbjct: 211 DESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGG 270

Query: 243 CFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGP 302
           CF SDILA +D+AI+D V+VLS+SLGGG + YYRD +AIGAFAAME+G+ VSCSAGN+GP
Sbjct: 271 CFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGP 330

Query: 303 NRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVY--DK 362
           + +SL+NVAPWI TVGAGTLDRDFPA   LGNGK FTGVSL+ G+ + +K +  +Y  + 
Sbjct: 331 SSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNA 390

Query: 363 GSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEEL 422
            + T+ N+C+ G+L P  V+GK+V+CDRGINARV+KG VV+ AGG+GMILANTA++GEEL
Sbjct: 391 SNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEEL 450

Query: 423 VADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLV 482
           VAD+HLLPA  VG K GD+IR YV +   PTA +S  GTV+ V+PSPVVAAFSSRGPN +
Sbjct: 451 VADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSI 510

Query: 483 TPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKA 542
           TP ILKPD+I PGVNILAAW+ + GPTGL SD R+ +FNI+SGTSMSCPH+SGLAALLK+
Sbjct: 511 TPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKS 570

Query: 543 AHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYD 602
            HP+WSP+AI+SALMTTAY        L D A G  S P+ HGAGHV P  A +PGL+YD
Sbjct: 571 VHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYD 630

Query: 603 ISTEDYIAFLCSLDYGIDHVQAIVKRPNITCS-RKFADPGQLNYPSFSVVFGSKRVVRYT 662
           ++TEDY+ FLC+L+Y    ++++ +R N TC   K      LNYPSF+V        +YT
Sbjct: 631 LTTEDYLGFLCALNYTSPQIRSVSRR-NYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYT 690

Query: 663 RIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFG 722
           R VT+VG AG+     T+  + VK+ V+P  L F +  E+K YTVTF   S+    +   
Sbjct: 691 RTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-S 750

Query: 723 FGSIAWSNDQHQVRSPVAFAWT 739
           FGSI WS+ +H V SPVA +WT
Sbjct: 751 FGSIEWSDGKHVVGSPVAISWT 757

BLAST of MS023139 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 4.9e-222
Identity = 408/746 (54.69%), Postives = 513/746 (68.77%), Query Frame = 0

Query: 3   TYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEAEL 62
           +YIV ++    PS + +H++W+ + L+SL SS    +LLY+Y+ A HGF+A L P +   
Sbjct: 32  SYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYSRAVHGFSARLSPIQTAA 91

Query: 63  LRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDTGI 122
           LR+  SV+ V  D    +HTT TP FLG   + GLW   N         DVI+GVLDTGI
Sbjct: 92  LRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSN------YGEDVIVGVLDTGI 151

Query: 123 WPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKP 182
           WPE  SF D+G+  IPS W+GECE GPDF  S CN+KLIGAR+F +GY     G      
Sbjct: 152 WPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAA 211

Query: 183 RENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFG 242
           +E++SPRD +GHGTHTASTAAGS VANASL  YARG A GMA +AR+AAYK CW  GC+ 
Sbjct: 212 KESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYD 271

Query: 243 SDILAGMDRAISDGVDVLSLSLG-GGSAP-YYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 302
           SDILA MD+A++DGV V+SLS+G  GSAP Y+ D+IAIGAF A   G+ VSCSAGNSGPN
Sbjct: 272 SDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPN 331

Query: 303 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 362
             +  N+APWI+TVGA T+DR+F A    G+GK FTG SLY+G+ + +  + LVY    +
Sbjct: 332 PETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVY--SGD 391

Query: 363 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 422
             S +C PG L  ++V GK+V+CDRG NARVEKG  V+ AGG GMILANTA SGEEL AD
Sbjct: 392 CGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTAD 451

Query: 423 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVR-PSPVVAAFSSRGPNLVTP 482
           SHL+PA  VG K GD IR Y+K+   PTA +SF GT++    PSP VAAFSSRGPN +TP
Sbjct: 452 SHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTP 511

Query: 483 QILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAH 542
            ILKPDVI PGVNILA W+  +GPT LD D R+ QFNI+SGTSMSCPH+SGLAALL+ AH
Sbjct: 512 VILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAH 571

Query: 543 PQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIS 602
           P WSP+AIKSAL+TTAY  +N+   + D A G  SN + HGAGHVDP+KAL+PGL+YDI 
Sbjct: 572 PDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIE 631

Query: 603 TEDYIAFLCSLDYGIDHVQAIVKRPNI--TC-SRKFADPGQLNYPSFSVVFGSK-RVVRY 662
            ++Y+AFLC++ Y    +   ++ P +   C + K    G LNYPSFSVVF S   VV+Y
Sbjct: 632 VKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKY 691

Query: 663 TRIVTNVGA-AGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTF---VASSNAAQ 722
            R+V NVG+   +VYEV   +P+ V++ V P+KLAF+K      Y VTF   V       
Sbjct: 692 KRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGS 751

Query: 723 TTRFGFGSIAWSNDQHQVRSPVAFAW 738
                FGSI W++ +H V+SPVA  W
Sbjct: 752 VPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of MS023139 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 1.7e-214
Identity = 391/753 (51.93%), Postives = 504/753 (66.93%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDS------LLYTYTAAYHGFAAS 60
           K+TY++ M   A+P  Y  H  WYS+ + S++   + +       +LYTY  A+HG AA 
Sbjct: 34  KKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQ 93

Query: 61  LDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVI 120
           L  EEAE L + D V+ V  +T Y LHTTR+P FLGL+      E           HDV+
Sbjct: 94  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ----ESERVWAERVTDHDVV 153

Query: 121 IGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMAS 180
           +GVLDTGIWPES+SF+DTGM  +P+ WRG CE+G  F    CN+K++GAR F +GY+ A+
Sbjct: 154 VGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAAT 213

Query: 181 GGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKT 240
             G   +  E +SPRD+DGHGTHTA+T AGS V  A+L G+A G ARGMA +ARVAAYK 
Sbjct: 214 --GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKV 273

Query: 241 CWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSA 300
           CW  GCF SDIL+ +D+A++DGV VLS+SLGGG + Y RD+++I  F AME GVFVSCSA
Sbjct: 274 CWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSA 333

Query: 301 GNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGM--GNKPVE 360
           GN GP+  SL NV+PWI TVGA T+DRDFPA V++G  + F GVSLY G+ +   NK   
Sbjct: 334 GNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYP 393

Query: 361 LVYDKGSNTSS----NMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILA 420
           LVY  G N SS    + CL G+L+   V GK+VICDRG+  RV+KG VV+ AGGIGM+L 
Sbjct: 394 LVY-LGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLT 453

Query: 421 NTASSGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAA 480
           NTA++GEELVADSH+LPAVAVG K G LI+QY  +  K TA L   GT + ++PSPVVAA
Sbjct: 454 NTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAA 513

Query: 481 FSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHI 540
           FSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L SD R+ +FNI+SGTSMSCPH+
Sbjct: 514 FSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHV 573

Query: 541 SGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHK 600
           SG+AAL+K+ HP WSP+AIKSALMTTAY  DN    L DA+G A S+P+ HGAGH+DP +
Sbjct: 574 SGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLR 633

Query: 601 ALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFA-DPGQLNYPSFSVVF 660
           A  PGL+YDI  ++Y  FLC+ D     ++   K  N TC    A +PG LNYP+ S +F
Sbjct: 634 ATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALF 693

Query: 661 GSK---RVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFV 720
                 + +   R VTNVG   S Y+V+ +      V V+P  L FT   ++  YTVTF 
Sbjct: 694 PENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTF- 753

Query: 721 ASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAW 738
                 +  R  FG + W +  H+VRSPV   W
Sbjct: 754 --RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of MS023139 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 8.1e-209
Identity = 381/744 (51.21%), Postives = 504/744 (67.74%), Query Frame = 0

Query: 2   RTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEAE 61
           +T+I R+   ++PS + TH+ WYS      S       +++ Y   +HGF+A + P+EA+
Sbjct: 26  KTFIFRIDGGSMPSIFPTHYHWYSTEFAEES------RIVHVYHTVFHGFSAVVTPDEAD 85

Query: 62  LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDTG 121
            LR   +VL V+ED    LHTTR+P FLGL +  GLW      + D  S DVIIGV DTG
Sbjct: 86  NLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW-----SESDYGS-DVIIGVFDTG 145

Query: 122 IWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRK 181
           IWPE +SF D  +  IP RWRG CESG  FSP  CN+K+IGAR F+KG Q A  GG   K
Sbjct: 146 IWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGG-INK 205

Query: 182 PRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCW-PTGC 241
             E  SPRD DGHGTHT+STAAG H   AS+ GYA G+A+G+AP+AR+AAYK CW  +GC
Sbjct: 206 TVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGC 265

Query: 242 FGSDILAGMDRAISDGVDVLSLSLGGG---SAPYYRDTIAIGAFAAMEKGVFVSCSAGNS 301
             SDILA  D A+ DGVDV+S+S+GGG   ++PYY D IAIG++ A  KG+FVS SAGN 
Sbjct: 266 LDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNE 325

Query: 302 GPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVY-D 361
           GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G R  GVSLY+G  +  +   +VY  
Sbjct: 326 GPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPG 385

Query: 362 KGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEE 421
           K   +S+++C+  +L+P  VRGK+VICDRG + RV KG VV+ AGG+GMILAN AS+GE 
Sbjct: 386 KSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEG 445

Query: 422 LVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNL 481
           LV D+HL+PA AVG   GD I+ Y  S   P A + F GT++ ++P+PV+A+FS RGPN 
Sbjct: 446 LVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNG 505

Query: 482 VTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLK 541
           ++P+ILKPD+I PGVNILAAW++++GPTGL SD RKT+FNI+SGTSM+CPH+SG AALLK
Sbjct: 506 LSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLK 565

Query: 542 AAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLY 601
           +AHP WSP+ I+SA+MTT    DN+N SL D + G  + P+ +G+GH++  +A++PGL+Y
Sbjct: 566 SAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVY 625

Query: 602 DISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKR--VVR 661
           DI+ +DYI FLCS+ YG   +Q I + P    + +   PG LNYPS + VF + R  +V 
Sbjct: 626 DITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVS 685

Query: 662 YT--RIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQT 721
            T  R  TNVG A +VY     +P  V V VKP +L FT   +R+ Y VT   ++     
Sbjct: 686 KTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVL 745

Query: 722 TRFG--FGSIAW-SNDQHQVRSPV 734
              G  FGS+ W    +H VRSP+
Sbjct: 746 GETGAVFGSVTWFDGGKHVVRSPI 756

BLAST of MS023139 vs. ExPASy TrEMBL
Match: A0A6J1JIF3 (subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3 SV=1)

HSP 1 Score: 1423.7 bits (3684), Expect = 0.0e+00
Identity = 707/738 (95.80%), Postives = 723/738 (97.97%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           KRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS TSDSLLYTYT+AYHGFAASLDP+EA
Sbjct: 27  KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSATSDSLLYTYTSAYHGFAASLDPDEA 86

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT
Sbjct: 87  ELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 146

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 147 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 206

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           K REN+SPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 207 KSRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 266

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 267 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 326

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPV LVY+KGSN
Sbjct: 327 KASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVALVYNKGSN 386

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           +SSNMCLPGSLEPA VRGKVVICDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 387 SSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 446

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 447 SHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 506

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 507 ILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 566

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
            WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNPWAHGAGHVDPHKALSPGLLYDIST
Sbjct: 567 SWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFSNPWAHGAGHVDPHKALSPGLLYDIST 626

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYI FLCSLDYGIDHVQAIVKR NITCS+KFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 627 NDYITFLCSLDYGIDHVQAIVKRSNITCSKKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 686

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVYEVATTAPSVVKV VKP+KL F+KVGERKRYTVTFVAS +AA+TTR+GFGSI
Sbjct: 687 NVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGERKRYTVTFVASRDAARTTRYGFGSI 746

Query: 721 AWSNDQHQVRSPVAFAWT 739
           AWSNDQHQVRSPVAFAWT
Sbjct: 747 AWSNDQHQVRSPVAFAWT 764

BLAST of MS023139 vs. ExPASy TrEMBL
Match: A0A6J1H8F1 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE=3 SV=1)

HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 706/738 (95.66%), Postives = 723/738 (97.97%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           KRTYIVRMKHHALPSDYLTHHDWYSA+LQSLSSS TSDSLLYTYT+AYHGFAASLDP+EA
Sbjct: 27  KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSATSDSLLYTYTSAYHGFAASLDPDEA 86

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT
Sbjct: 87  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 146

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 147 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 206

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           K REN+SPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 207 KSRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 266

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 267 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 326

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPV LVY+KGSN
Sbjct: 327 KASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVALVYNKGSN 386

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           +SSNMCLPGSLEPA VRGKVVICDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 387 SSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 446

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVG KTGDLIRQYV+SV KPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 447 SHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 506

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 507 ILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 566

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
            WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGL+YDIST
Sbjct: 567 SWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLVYDIST 626

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYI FLCSLDYGIDHVQAI KR NITC +KFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 627 NDYITFLCSLDYGIDHVQAIAKRSNITCLKKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 686

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVYEVATTAPSVVKV VKP+KL F+KVGERKRYTVTFVAS +AA+TTR+GFGSI
Sbjct: 687 NVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGERKRYTVTFVASRDAARTTRYGFGSI 746

Query: 721 AWSNDQHQVRSPVAFAWT 739
           AWSNDQHQVRSPVAFAWT
Sbjct: 747 AWSNDQHQVRSPVAFAWT 764

BLAST of MS023139 vs. ExPASy TrEMBL
Match: A0A5D3CDV9 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001530 PE=3 SV=1)

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 696/738 (94.31%), Postives = 720/738 (97.56%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           K+TYIV MKHHALPS+YLTHHDWYSA+LQSLSSS++SDSLLYTYT+++HGFAA LD EE 
Sbjct: 25  KKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLYTYTSSFHGFAAFLDSEEV 84

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGH TQDL+QASHDVIIGVLDT
Sbjct: 85  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHTTQDLNQASHDVIIGVLDT 144

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRGECE+GPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 145 GIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 204

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           KPRENQS RDQDGHGTHTASTAAGSHV NASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 205 KPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIARGMAPQARVAAYKTCWPTGC 264

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 265 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 324

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNK V LVY+KGSN
Sbjct: 325 KASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKAVALVYNKGSN 384

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           TSSNMCLPGSL+PAVVRGKVV+CDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 385 TSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 444

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVGRKTGDLIRQYV+S + PTAVLSFGGT+LNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 445 SHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQ 504

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 505 ILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 564

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
           QWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNPWAHGAGHVDPHKALSPGLLYDIST
Sbjct: 565 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGHVDPHKALSPGLLYDIST 624

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYIAFLCSLDYGIDHVQAIVKR NITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 625 NDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 684

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVY+VATTAPSVVKV VKP+KL FTKVGERKRYTVTFVAS +AAQTTRFGFGSI
Sbjct: 685 NVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSI 744

Query: 721 AWSNDQHQVRSPVAFAWT 739
            WSNDQHQVRSPV+FAWT
Sbjct: 745 VWSNDQHQVRSPVSFAWT 762

BLAST of MS023139 vs. ExPASy TrEMBL
Match: A0A5A7UKV3 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001560 PE=3 SV=1)

HSP 1 Score: 1403.3 bits (3631), Expect = 0.0e+00
Identity = 695/738 (94.17%), Postives = 719/738 (97.43%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           K+TYIV MKHHALPS+YLTHHDWYSA+LQSLSSS++SDSLLYTYT+++HGFAA LD EE 
Sbjct: 26  KKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLYTYTSSFHGFAAFLDSEEV 85

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGH TQDL+QASHDVIIGVLDT
Sbjct: 86  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHTTQDLNQASHDVIIGVLDT 145

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 146 GIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 205

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           KPRENQS RDQDGHGTHTASTAAGSHV NASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 206 KPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIARGMAPQARVAAYKTCWPTGC 265

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 266 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 325

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNK V LVY+KGSN
Sbjct: 326 KASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKAVALVYNKGSN 385

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           TSSNMCLPGSL+PAVVRGKVV+CDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 386 TSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 445

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVGRKTGDLIRQYV+S + PTAVLSFGGT+LNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 446 SHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQ 505

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 506 ILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 565

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
           QWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNPWAHGAGHVDPHKALSPGLLYDIST
Sbjct: 566 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGHVDPHKALSPGLLYDIST 625

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYIAFLCSLDYGIDHVQAIVKR NITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 626 NDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 685

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVY+VATTAPSVVKV VKP+KL FTKVGERKRYTVTFVAS +AAQTTRFGFGSI
Sbjct: 686 NVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSI 745

Query: 721 AWSNDQHQVRSPVAFAWT 739
            WSNDQHQVRSPV+FAWT
Sbjct: 746 VWSNDQHQVRSPVSFAWT 763

BLAST of MS023139 vs. ExPASy TrEMBL
Match: A0A1S3BQE8 (subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=1)

HSP 1 Score: 1403.3 bits (3631), Expect = 0.0e+00
Identity = 695/738 (94.17%), Postives = 719/738 (97.43%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           K+TYIV MKHHALPS+YLTHHDWYSA+LQSLSSS++SDSLLYTYT+++HGFAA LD EE 
Sbjct: 26  KKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLYTYTSSFHGFAAFLDSEEV 85

Query: 61  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDT 120
           ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGH TQDL+QASHDVIIGVLDT
Sbjct: 86  ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHTTQDLNQASHDVIIGVLDT 145

Query: 121 GIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 180
           GIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR
Sbjct: 146 GIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFR 205

Query: 181 KPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGC 240
           KPRENQS RDQDGHGTHTASTAAGSHV NASLLGYARGIARGMAPQARVAAYKTCWPTGC
Sbjct: 206 KPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIARGMAPQARVAAYKTCWPTGC 265

Query: 241 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 300
           FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN
Sbjct: 266 FGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 325

Query: 301 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 360
           +ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNK V LVY+KGSN
Sbjct: 326 KASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKAVALVYNKGSN 385

Query: 361 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 420
           TSSNMCLPGSL+PAVVRGKVV+CDRGINARVEKGGVVRDAGGIGMILANTA+SGEELVAD
Sbjct: 386 TSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 445

Query: 421 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 480
           SHLLPAVAVGRKTGDLIRQYV+S + PTAVLSFGGT+LNVRPSPVVAAFSSRGPNLVTPQ
Sbjct: 446 SHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQ 505

Query: 481 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 540
           ILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQFNIMSGTSMSCPHISGLAALLKAAHP
Sbjct: 506 ILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 565

Query: 541 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIST 600
           QWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNPWAHGAGHVDPHKALSPGLLYDIST
Sbjct: 566 QWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGHVDPHKALSPGLLYDIST 625

Query: 601 EDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 660
            DYIAFLCSLDYGIDHVQAIVKR NITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT
Sbjct: 626 NDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVT 685

Query: 661 NVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGSI 720
           NVGAAGSVY+VATTAPSVVKV VKP+KL FTKVGERKRYTVTFVAS +AAQTTRFGFGSI
Sbjct: 686 NVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSI 745

Query: 721 AWSNDQHQVRSPVAFAWT 739
            WSNDQHQVRSPV+FAWT
Sbjct: 746 VWSNDQHQVRSPVSFAWT 763

BLAST of MS023139 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 543/738 (73.58%), Postives = 629/738 (85.23%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEA 60
           K+TYI+R+ H   P  +LTHHDWY++ L S S      SLLYTYT ++HGF+A LD  EA
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 61  E-LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLD 120
           + LL  S+S+L ++ED +Y LHTTRTP FLGL+S+FG+       DL  +S+ VIIGVLD
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------HDLGSSSNGVIIGVLD 146

Query: 121 TGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYF 180
           TG+WPES+SFDDT MPEIPS+W+GECESG DF   LCNKKLIGARSFSKG+QMASGGG+ 
Sbjct: 147 TGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFS 206

Query: 181 RKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTG 240
            K RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G ARGMA +ARVA YK CW TG
Sbjct: 207 SK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTG 266

Query: 241 CFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGP 300
           CFGSDILA MDRAI DGVDVLSLSLGGGSAPYYRDTIAIGAF+AME+GVFVSCSAGNSGP
Sbjct: 267 CFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGP 326

Query: 301 NRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGS 360
            RAS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG GMG KP+ELVY+KG+
Sbjct: 327 TRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGN 386

Query: 361 NTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVA 420
           ++SSN+CLPGSL+ ++VRGK+V+CDRG+NARVEKG VVRDAGG+GMI+ANTA+SGEELVA
Sbjct: 387 SSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVA 446

Query: 421 DSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTP 480
           DSHLLPA+AVG+KTGDL+R+YVKS +KPTA+L F GTVL+V+PSPVVAAFSSRGPN VTP
Sbjct: 447 DSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTP 506

Query: 481 QILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAH 540
           +ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSGTSMSCPHISGLA LLKAAH
Sbjct: 507 EILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAH 566

Query: 541 PQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIS 600
           P+WSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GHVDP KALSPGL+YDIS
Sbjct: 567 PEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDIS 626

Query: 601 TEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIV 660
           TE+YI FLCSLDY +DH+ AIVKRP++ CS+KF+DPGQLNYPSFSV+FG KRVVRYTR V
Sbjct: 627 TEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREV 686

Query: 661 TNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFGFGS 720
           TNVGAA SVY+V       V + VKP+KL+F  VGE+KRYTVTFV+    + T +  FGS
Sbjct: 687 TNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGS 746

Query: 721 IAWSNDQHQVRSPVAFAW 738
           I WSN QH+VRSPVAF+W
Sbjct: 747 ITWSNPQHEVRSPVAFSW 751

BLAST of MS023139 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 810.1 bits (2091), Expect = 1.5e-234
Identity = 422/742 (56.87%), Postives = 533/742 (71.83%), Query Frame = 0

Query: 3   TYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEAEL 62
           TYIV M    +PS +  H +WY ++L+S+S S     LLYTY  A HGF+  L  EEA+ 
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA---ELLYTYENAIHGFSTRLTQEEADS 90

Query: 63  LRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDL--DQASH-DVIIGVLD 122
           L     V+ V  +  Y LHTTRTP FLGLD         +T DL  +  S+ DV++GVLD
Sbjct: 91  LMTQPGVISVLPEHRYELHTTRTPLFLGLD--------EHTADLFPEAGSYSDVVVGVLD 150

Query: 123 TGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYF 182
           TG+WPESKS+ D G   IPS W+G CE+G +F+ SLCN+KLIGAR F++GY+  S  G  
Sbjct: 151 TGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE--STMGPI 210

Query: 183 RKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTG 242
            + +E++SPRD DGHGTHT+STAAGS V  ASLLGYA G ARGMAP+ARVA YK CW  G
Sbjct: 211 DESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGG 270

Query: 243 CFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGP 302
           CF SDILA +D+AI+D V+VLS+SLGGG + YYRD +AIGAFAAME+G+ VSCSAGN+GP
Sbjct: 271 CFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGP 330

Query: 303 NRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVY--DK 362
           + +SL+NVAPWI TVGAGTLDRDFPA   LGNGK FTGVSL+ G+ + +K +  +Y  + 
Sbjct: 331 SSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNA 390

Query: 363 GSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEEL 422
            + T+ N+C+ G+L P  V+GK+V+CDRGINARV+KG VV+ AGG+GMILANTA++GEEL
Sbjct: 391 SNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEEL 450

Query: 423 VADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLV 482
           VAD+HLLPA  VG K GD+IR YV +   PTA +S  GTV+ V+PSPVVAAFSSRGPN +
Sbjct: 451 VADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSI 510

Query: 483 TPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKA 542
           TP ILKPD+I PGVNILAAW+ + GPTGL SD R+ +FNI+SGTSMSCPH+SGLAALLK+
Sbjct: 511 TPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKS 570

Query: 543 AHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYD 602
            HP+WSP+AI+SALMTTAY        L D A G  S P+ HGAGHV P  A +PGL+YD
Sbjct: 571 VHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYD 630

Query: 603 ISTEDYIAFLCSLDYGIDHVQAIVKRPNITCS-RKFADPGQLNYPSFSVVFGSKRVVRYT 662
           ++TEDY+ FLC+L+Y    ++++ +R N TC   K      LNYPSF+V        +YT
Sbjct: 631 LTTEDYLGFLCALNYTSPQIRSVSRR-NYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYT 690

Query: 663 RIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQTTRFG 722
           R VT+VG AG+     T+  + VK+ V+P  L F +  E+K YTVTF   S+    +   
Sbjct: 691 RTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-S 750

Query: 723 FGSIAWSNDQHQVRSPVAFAWT 739
           FGSI WS+ +H V SPVA +WT
Sbjct: 751 FGSIEWSDGKHVVGSPVAISWT 757

BLAST of MS023139 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 772.3 bits (1993), Expect = 3.5e-223
Identity = 408/746 (54.69%), Postives = 513/746 (68.77%), Query Frame = 0

Query: 3   TYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEAEL 62
           +YIV ++    PS + +H++W+ + L+SL SS    +LLY+Y+ A HGF+A L P +   
Sbjct: 32  SYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYSRAVHGFSARLSPIQTAA 91

Query: 63  LRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDTGI 122
           LR+  SV+ V  D    +HTT TP FLG   + GLW   N         DVI+GVLDTGI
Sbjct: 92  LRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSN------YGEDVIVGVLDTGI 151

Query: 123 WPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKP 182
           WPE  SF D+G+  IPS W+GECE GPDF  S CN+KLIGAR+F +GY     G      
Sbjct: 152 WPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAA 211

Query: 183 RENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFG 242
           +E++SPRD +GHGTHTASTAAGS VANASL  YARG A GMA +AR+AAYK CW  GC+ 
Sbjct: 212 KESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYD 271

Query: 243 SDILAGMDRAISDGVDVLSLSLG-GGSAP-YYRDTIAIGAFAAMEKGVFVSCSAGNSGPN 302
           SDILA MD+A++DGV V+SLS+G  GSAP Y+ D+IAIGAF A   G+ VSCSAGNSGPN
Sbjct: 272 SDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPN 331

Query: 303 RASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVYDKGSN 362
             +  N+APWI+TVGA T+DR+F A    G+GK FTG SLY+G+ + +  + LVY    +
Sbjct: 332 PETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVY--SGD 391

Query: 363 TSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEELVAD 422
             S +C PG L  ++V GK+V+CDRG NARVEKG  V+ AGG GMILANTA SGEEL AD
Sbjct: 392 CGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTAD 451

Query: 423 SHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVR-PSPVVAAFSSRGPNLVTP 482
           SHL+PA  VG K GD IR Y+K+   PTA +SF GT++    PSP VAAFSSRGPN +TP
Sbjct: 452 SHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTP 511

Query: 483 QILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAH 542
            ILKPDVI PGVNILA W+  +GPT LD D R+ QFNI+SGTSMSCPH+SGLAALL+ AH
Sbjct: 512 VILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAH 571

Query: 543 PQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLYDIS 602
           P WSP+AIKSAL+TTAY  +N+   + D A G  SN + HGAGHVDP+KAL+PGL+YDI 
Sbjct: 572 PDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIE 631

Query: 603 TEDYIAFLCSLDYGIDHVQAIVKRPNI--TC-SRKFADPGQLNYPSFSVVFGSK-RVVRY 662
            ++Y+AFLC++ Y    +   ++ P +   C + K    G LNYPSFSVVF S   VV+Y
Sbjct: 632 VKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKY 691

Query: 663 TRIVTNVGA-AGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTF---VASSNAAQ 722
            R+V NVG+   +VYEV   +P+ V++ V P+KLAF+K      Y VTF   V       
Sbjct: 692 KRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGS 751

Query: 723 TTRFGFGSIAWSNDQHQVRSPVAFAW 738
                FGSI W++ +H V+SPVA  W
Sbjct: 752 VPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of MS023139 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 747.3 bits (1928), Expect = 1.2e-215
Identity = 391/753 (51.93%), Postives = 504/753 (66.93%), Query Frame = 0

Query: 1   KRTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDS------LLYTYTAAYHGFAAS 60
           K+TY++ M   A+P  Y  H  WYS+ + S++   + +       +LYTY  A+HG AA 
Sbjct: 34  KKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQ 93

Query: 61  LDPEEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVI 120
           L  EEAE L + D V+ V  +T Y LHTTR+P FLGL+      E           HDV+
Sbjct: 94  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ----ESERVWAERVTDHDVV 153

Query: 121 IGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMAS 180
           +GVLDTGIWPES+SF+DTGM  +P+ WRG CE+G  F    CN+K++GAR F +GY+ A+
Sbjct: 154 VGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAAT 213

Query: 181 GGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKT 240
             G   +  E +SPRD+DGHGTHTA+T AGS V  A+L G+A G ARGMA +ARVAAYK 
Sbjct: 214 --GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKV 273

Query: 241 CWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSA 300
           CW  GCF SDIL+ +D+A++DGV VLS+SLGGG + Y RD+++I  F AME GVFVSCSA
Sbjct: 274 CWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSA 333

Query: 301 GNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGM--GNKPVE 360
           GN GP+  SL NV+PWI TVGA T+DRDFPA V++G  + F GVSLY G+ +   NK   
Sbjct: 334 GNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYP 393

Query: 361 LVYDKGSNTSS----NMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILA 420
           LVY  G N SS    + CL G+L+   V GK+VICDRG+  RV+KG VV+ AGGIGM+L 
Sbjct: 394 LVY-LGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLT 453

Query: 421 NTASSGEELVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAA 480
           NTA++GEELVADSH+LPAVAVG K G LI+QY  +  K TA L   GT + ++PSPVVAA
Sbjct: 454 NTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAA 513

Query: 481 FSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHI 540
           FSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L SD R+ +FNI+SGTSMSCPH+
Sbjct: 514 FSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHV 573

Query: 541 SGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHK 600
           SG+AAL+K+ HP WSP+AIKSALMTTAY  DN    L DA+G A S+P+ HGAGH+DP +
Sbjct: 574 SGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLR 633

Query: 601 ALSPGLLYDISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFA-DPGQLNYPSFSVVF 660
           A  PGL+YDI  ++Y  FLC+ D     ++   K  N TC    A +PG LNYP+ S +F
Sbjct: 634 ATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALF 693

Query: 661 GSK---RVVRYTRIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFV 720
                 + +   R VTNVG   S Y+V+ +      V V+P  L FT   ++  YTVTF 
Sbjct: 694 PENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTF- 753

Query: 721 ASSNAAQTTRFGFGSIAWSNDQHQVRSPVAFAW 738
                 +  R  FG + W +  H+VRSPV   W
Sbjct: 754 --RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of MS023139 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 728.4 bits (1879), Expect = 5.7e-210
Identity = 381/744 (51.21%), Postives = 504/744 (67.74%), Query Frame = 0

Query: 2   RTYIVRMKHHALPSDYLTHHDWYSANLQSLSSSTTSDSLLYTYTAAYHGFAASLDPEEAE 61
           +T+I R+   ++PS + TH+ WYS      S       +++ Y   +HGF+A + P+EA+
Sbjct: 26  KTFIFRIDGGSMPSIFPTHYHWYSTEFAEES------RIVHVYHTVFHGFSAVVTPDEAD 85

Query: 62  LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDTG 121
            LR   +VL V+ED    LHTTR+P FLGL +  GLW      + D  S DVIIGV DTG
Sbjct: 86  NLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW-----SESDYGS-DVIIGVFDTG 145

Query: 122 IWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRK 181
           IWPE +SF D  +  IP RWRG CESG  FSP  CN+K+IGAR F+KG Q A  GG   K
Sbjct: 146 IWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGG-INK 205

Query: 182 PRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCW-PTGC 241
             E  SPRD DGHGTHT+STAAG H   AS+ GYA G+A+G+AP+AR+AAYK CW  +GC
Sbjct: 206 TVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGC 265

Query: 242 FGSDILAGMDRAISDGVDVLSLSLGGG---SAPYYRDTIAIGAFAAMEKGVFVSCSAGNS 301
             SDILA  D A+ DGVDV+S+S+GGG   ++PYY D IAIG++ A  KG+FVS SAGN 
Sbjct: 266 LDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNE 325

Query: 302 GPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVELVY-D 361
           GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G R  GVSLY+G  +  +   +VY  
Sbjct: 326 GPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPG 385

Query: 362 KGSNTSSNMCLPGSLEPAVVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTASSGEE 421
           K   +S+++C+  +L+P  VRGK+VICDRG + RV KG VV+ AGG+GMILAN AS+GE 
Sbjct: 386 KSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEG 445

Query: 422 LVADSHLLPAVAVGRKTGDLIRQYVKSVTKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNL 481
           LV D+HL+PA AVG   GD I+ Y  S   P A + F GT++ ++P+PV+A+FS RGPN 
Sbjct: 446 LVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNG 505

Query: 482 VTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLK 541
           ++P+ILKPD+I PGVNILAAW++++GPTGL SD RKT+FNI+SGTSM+CPH+SG AALLK
Sbjct: 506 LSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLK 565

Query: 542 AAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLLY 601
           +AHP WSP+ I+SA+MTT    DN+N SL D + G  + P+ +G+GH++  +A++PGL+Y
Sbjct: 566 SAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVY 625

Query: 602 DISTEDYIAFLCSLDYGIDHVQAIVKRPNITCSRKFADPGQLNYPSFSVVFGSKR--VVR 661
           DI+ +DYI FLCS+ YG   +Q I + P    + +   PG LNYPS + VF + R  +V 
Sbjct: 626 DITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVS 685

Query: 662 YT--RIVTNVGAAGSVYEVATTAPSVVKVVVKPTKLAFTKVGERKRYTVTFVASSNAAQT 721
            T  R  TNVG A +VY     +P  V V VKP +L FT   +R+ Y VT   ++     
Sbjct: 686 KTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVL 745

Query: 722 TRFG--FGSIAW-SNDQHQVRSPV 734
              G  FGS+ W    +H VRSP+
Sbjct: 746 GETGAVFGSVTWFDGGKHVVRSPI 756