MS023027 (gene) Bitter gourd (TR) v1

BLAST of MS023027 vs. NCBI nr
Match: XP_022154218.1 (OTU domain-containing protein At3g57810 isoform X1 [Momordica charantia])

HSP 1 Score: 688.0 bits (1774), Expect = 4.2e-194
Identity = 332/338 (98.22%), Postives = 332/338 (98.22%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           MNIGSISACTRNVVHLNSRVPMQMGSNICSVLS R SNSCCSYGISRPKYADQSVTTISS
Sbjct: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSPRISNSCCSYGISRPKYADQSVTTISS 60

Query: 61  CTPNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIP 120
           CTPNTSQRFP GCLSYCFSRQPIDFKNLTVKDLIT GGSHGRGLEISLACKGMNVKLSI 
Sbjct: 61  CTPNTSQRFPGGCLSYCFSRQPIDFKNLTVKDLITDGGSHGRGLEISLACKGMNVKLSIT 120

Query: 121 NDGFSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFSH 180
           NDGFSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFSH
Sbjct: 121 NDGFSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFSH 180

Query: 181 GKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIKR 240
           GKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGK APSESLQRDLADELRNKVADEFIKR
Sbjct: 181 GKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKAAPSESLQRDLADELRNKVADEFIKR 240

Query: 241 RKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEY 300
           RKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEY
Sbjct: 241 RKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEY 300

Query: 301 GDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           GDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL
Sbjct: 301 GDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 338

BLAST of MS023027 vs. NCBI nr
Match: XP_008443087.1 (PREDICTED: OTU domain-containing protein At3g57810 isoform X1 [Cucumis melo] >XP_008443088.1 PREDICTED: OTU domain-containing protein At3g57810 isoform X1 [Cucumis melo] >XP_008443089.1 PREDICTED: OTU domain-containing protein At3g57810 isoform X1 [Cucumis melo] >XP_008443090.1 PREDICTED: OTU domain-containing protein At3g57810 isoform X1 [Cucumis melo] >KAA0043710.1 OTU domain-containing protein [Cucumis melo var. makuwa] >TYK25424.1 OTU domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 599.4 bits (1544), Expect = 2.0e-167
Identity = 296/339 (87.32%), Postives = 311/339 (91.74%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           M+IGSIS CTRN V LN R+  QMGSNIC+VLSRRTS SCCSYGISRPKYADQSVTTISS
Sbjct: 1   MSIGSISICTRNAVPLNLRIYTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60

Query: 61  CTPNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIP 120
           C+PNTSQRF  GCLS CFSR+ IDF+  TVKDLIT GGS GR +EISLACKGMNVKLSIP
Sbjct: 61  CSPNTSQRFQGGCLSTCFSRRSIDFQAFTVKDLITDGGSPGREVEISLACKGMNVKLSIP 120

Query: 121 NDG-FSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFS 180
           NDG FSKIK+NMRWPER ASAGLVFGWVVCYS+SEPVHAEAA EK+DNEENSDSS VK S
Sbjct: 121 NDGTFSKIKYNMRWPERWASAGLVFGWVVCYSTSEPVHAEAAYEKDDNEENSDSSHVKLS 180

Query: 181 HGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIK 240
           HGK VYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELR+ VADEFIK
Sbjct: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRSNVADEFIK 240

Query: 241 RRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300
           RR+ETEWFVEGDFD YVSNMRKPH+WGGEPELFMASHVL APIIVYMYDKDSGGLISIAE
Sbjct: 241 RREETEWFVEGDFDTYVSNMRKPHVWGGEPELFMASHVLQAPIIVYMYDKDSGGLISIAE 300

Query: 301 YGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           YGDEYGKENPIRVLYHGFGHYDALQIP  A++GV RSKL
Sbjct: 301 YGDEYGKENPIRVLYHGFGHYDALQIP--ANQGVGRSKL 337

BLAST of MS023027 vs. NCBI nr
Match: XP_011652109.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Cucumis sativus] >XP_011652110.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Cucumis sativus] >XP_011652111.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 588.6 bits (1516), Expect = 3.5e-164
Identity = 294/340 (86.47%), Postives = 309/340 (90.88%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           M+IGSIS CTRN V LN R   QMGSNIC+VLSRRTS SCCSYGISRPKYADQSVTTISS
Sbjct: 1   MSIGSISICTRNAVPLNLRT--QMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60

Query: 61  CTPNTSQRFPE-GCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSI 120
           C+PNTSQRF   GCLS CFSR+ IDF+  TVKDLIT GGS GR +EISLACKGMNVKLSI
Sbjct: 61  CSPNTSQRFQAGGCLSTCFSRRSIDFQAFTVKDLITDGGSRGRDVEISLACKGMNVKLSI 120

Query: 121 PNDG-FSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKF 180
           PNDG FSKIK+NMRWPER ASAGLVFGWVVCYS+SEPVHAEAA EK+DNEENSDSS VK 
Sbjct: 121 PNDGTFSKIKYNMRWPERWASAGLVFGWVVCYSTSEPVHAEAAYEKDDNEENSDSSHVKL 180

Query: 181 SHGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFI 240
           SHGK VYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELR+ VADEFI
Sbjct: 181 SHGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRSNVADEFI 240

Query: 241 KRRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIA 300
           KRR+ETEWFVEGDFD Y+SNMR PH+WGGEPELFMASHVL APIIVYMYDKDSGGLISIA
Sbjct: 241 KRREETEWFVEGDFDTYMSNMRNPHVWGGEPELFMASHVLQAPIIVYMYDKDSGGLISIA 300

Query: 301 EYGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           EYGDEYGKENPIRVLYHGFGHYDALQIP  A++GV RSKL
Sbjct: 301 EYGDEYGKENPIRVLYHGFGHYDALQIP--ANQGVGRSKL 336

BLAST of MS023027 vs. NCBI nr
Match: XP_022935652.1 (OTU domain-containing protein At3g57810 isoform X2 [Cucurbita moschata] >XP_022935653.1 OTU domain-containing protein At3g57810 isoform X2 [Cucurbita moschata])

HSP 1 Score: 572.8 bits (1475), Expect = 2.0e-159
Identity = 282/341 (82.70%), Postives = 302/341 (88.56%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           M+IGSIS CTRNV+ LN RV  QMGSNIC+VLS RT  SCCSYGISRPKY DQSVT ISS
Sbjct: 1   MSIGSISVCTRNVIPLNFRVCTQMGSNICTVLSGRTPTSCCSYGISRPKYGDQSVTIISS 60

Query: 61  CTPNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIP 120
           CTPNT QR   GCLSYCFSR+P DF+ LTVKDLIT GGS GRGLEISLACKGMN K+S+P
Sbjct: 61  CTPNTCQRIQGGCLSYCFSRRPNDFEALTVKDLITDGGSRGRGLEISLACKGMNAKISVP 120

Query: 121 NDGF-SKIKHNMRWPERC--ASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVK 180
           +DG  SKIK+NMRWPERC  ASAGLVFGWVVC S+SEPVHAEAA +K+ NEEN+DSS VK
Sbjct: 121 SDGMCSKIKYNMRWPERCASASAGLVFGWVVCCSTSEPVHAEAAYDKKGNEENNDSSHVK 180

Query: 181 FSHGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEF 240
           FSHGK VY DYSVIGIPGDGRCLFRSVAHGACLRSGK AP+ESLQR+LADELR KVADEF
Sbjct: 181 FSHGKKVYIDYSVIGIPGDGRCLFRSVAHGACLRSGKSAPTESLQRELADELRTKVADEF 240

Query: 241 IKRRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 300
           IKRR+ETEWFVEGDFD YVSNMRKPHIWGGEPELFMASHVL APIIVYMYDKD+GGLISI
Sbjct: 241 IKRREETEWFVEGDFDTYVSNMRKPHIWGGEPELFMASHVLQAPIIVYMYDKDAGGLISI 300

Query: 301 AEYGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           AEYGDEYGK+NPI+VLYHGFGHYDALQIPA   +   RSKL
Sbjct: 301 AEYGDEYGKDNPIKVLYHGFGHYDALQIPANQGQ-AGRSKL 340

BLAST of MS023027 vs. NCBI nr
Match: XP_022935651.1 (OTU domain-containing protein At3g57810 isoform X1 [Cucurbita moschata])

HSP 1 Score: 572.8 bits (1475), Expect = 2.0e-159
Identity = 282/341 (82.70%), Postives = 302/341 (88.56%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           M+IGSIS CTRNV+ LN RV  QMGSNIC+VLS RT  SCCSYGISRPKY DQSVT ISS
Sbjct: 13  MSIGSISVCTRNVIPLNFRVCTQMGSNICTVLSGRTPTSCCSYGISRPKYGDQSVTIISS 72

Query: 61  CTPNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIP 120
           CTPNT QR   GCLSYCFSR+P DF+ LTVKDLIT GGS GRGLEISLACKGMN K+S+P
Sbjct: 73  CTPNTCQRIQGGCLSYCFSRRPNDFEALTVKDLITDGGSRGRGLEISLACKGMNAKISVP 132

Query: 121 NDGF-SKIKHNMRWPERC--ASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVK 180
           +DG  SKIK+NMRWPERC  ASAGLVFGWVVC S+SEPVHAEAA +K+ NEEN+DSS VK
Sbjct: 133 SDGMCSKIKYNMRWPERCASASAGLVFGWVVCCSTSEPVHAEAAYDKKGNEENNDSSHVK 192

Query: 181 FSHGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEF 240
           FSHGK VY DYSVIGIPGDGRCLFRSVAHGACLRSGK AP+ESLQR+LADELR KVADEF
Sbjct: 193 FSHGKKVYIDYSVIGIPGDGRCLFRSVAHGACLRSGKSAPTESLQRELADELRTKVADEF 252

Query: 241 IKRRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 300
           IKRR+ETEWFVEGDFD YVSNMRKPHIWGGEPELFMASHVL APIIVYMYDKD+GGLISI
Sbjct: 253 IKRREETEWFVEGDFDTYVSNMRKPHIWGGEPELFMASHVLQAPIIVYMYDKDAGGLISI 312

Query: 301 AEYGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           AEYGDEYGK+NPI+VLYHGFGHYDALQIPA   +   RSKL
Sbjct: 313 AEYGDEYGKDNPIKVLYHGFGHYDALQIPANQGQ-AGRSKL 352

BLAST of MS023027 vs. ExPASy Swiss-Prot
Match: Q8LBZ4 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 OS=Arabidopsis thaliana OX=3702 GN=OTU4 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.4e-75
Identity = 167/340 (49.12%), Postives = 212/340 (62.35%), Query Frame = 0

Query: 6   ISACTRNVVHLNSRVPMQMGSNICSVLSRRTSN-SCCSY--GISRPKYADQSVTTISSCT 65
           I+ C+RN +     +   +GS +  V++  +S  SC S   G+SR  Y    V    S +
Sbjct: 8   ITTCSRNAI----SIKRHLGSRLYGVVAHGSSKFSCYSLLSGLSRRHYTGFRV----SVS 67

Query: 66  PNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIPND 125
              S    +G        +P      TV        +    LE+S      N+K      
Sbjct: 68  NRPSSWHDKGLFGSVLINRP------TV--------APKEKLEVSFLSPEANMKC----- 127

Query: 126 GFSKIKHNMR---WPERCASAGLVFGWVVCYSS-SEPVHAEAACEKEDNEENSDSSPVKF 185
             SKI+ NMR      R A  G++   +VCYSS S+  +A+++ +K+ N  +  SS  KF
Sbjct: 128 --SKIESNMRNLYCYSRFAYTGVIVSLLVCYSSTSQSAYADSSRDKDANNVHHHSSDGKF 187

Query: 186 SHGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFI 245
            +GK VYTDYS+IGIPGDGRCLFRSVAHG CLRSGK AP E +QR+LADELR +VADEFI
Sbjct: 188 HNGKRVYTDYSIIGIPGDGRCLFRSVAHGFCLRSGKLAPGEKMQRELADELRTRVADEFI 247

Query: 246 KRRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIA 305
           +RR+ETEWFVEGDFD YV  +R PH+WGGEPELFMASHVL  PI VYM D  +GGLISIA
Sbjct: 248 QRRQETEWFVEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDDKAGGLISIA 307

Query: 306 EYGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           EYG EYGK++PIRVLYHGFGHYDAL +  + +  + +SKL
Sbjct: 308 EYGQEYGKDDPIRVLYHGFGHYDALLLHESKA-SIPKSKL 317

BLAST of MS023027 vs. ExPASy Swiss-Prot
Match: Q8GYW0 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=OTU3 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.7e-15
Identity = 56/160 (35.00%), Postives = 78/160 (48.75%), Query Frame = 0

Query: 188 YSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIKRRKETEWF 247
           Y+V  + GDGRCLFR++  G     G    +   +RD ADELR  V +      KE E +
Sbjct: 76  YAVDRVKGDGRCLFRALVKGMAFNKGITL-NPQRERDDADELRMAVKEVICNDPKEREKY 135

Query: 248 --------VEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSG------- 307
                   V+    R+   + +   WGGE EL + S +   PIIVY+ + + G       
Sbjct: 136 KEALVAITVDESLKRFCQRIGRHDFWGGESELLVLSKLCKQPIIVYIPEHEHGRGGGYGP 195

Query: 308 GLISIAEYGDEY------GK--ENPIRVLYHGFGHYDALQ 325
           G I I EYG E+      GK  +N +R+LY G  HYD L+
Sbjct: 196 GFIPIQEYGSEFRGGWGKGKTNKNVVRLLYSGRNHYDLLR 234

BLAST of MS023027 vs. ExPASy Swiss-Prot
Match: Q96G74 (OTU domain-containing protein 5 OS=Homo sapiens OX=9606 GN=OTUD5 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.8e-07
Identity = 38/139 (27.34%), Postives = 62/139 (44.60%), Query Frame = 0

Query: 196 DGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIKRRKETEWFVEGDFDRY 255
           DG CLFR+V           A      +D+ + +R    D  +K       +V  DF  Y
Sbjct: 221 DGACLFRAV-----------ADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTY 280

Query: 256 VSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEY-GDEYGKENPIRVLY 315
           ++  RK +  G   E+   + + + P+ VY Y   +  +  I  + G    ++ PIRV Y
Sbjct: 281 INRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTGTSAVEPINTFHGIHQNEDEPIRVSY 340

Query: 316 HGFGHYDALQIPATASKGV 334
           H   HY+++  P  A+ GV
Sbjct: 341 HRNIHYNSVVNPNKATIGV 348

BLAST of MS023027 vs. ExPASy Swiss-Prot
Match: Q640H3 (OTU domain-containing protein 5-B OS=Xenopus laevis OX=8355 GN=otud5-b PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.4e-06
Identity = 37/138 (26.81%), Postives = 61/138 (44.20%), Query Frame = 0

Query: 196 DGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIKRRKETEWFVEGDFDRY 255
           DG CLFR+V           A      +D+ + +R    D  +K       +V  DF  Y
Sbjct: 179 DGACLFRAV-----------ADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTY 238

Query: 256 VSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEYGDEYGKENPIRVLYH 315
           ++  RK +  G   E+   + + + P+ VY Y  +         +G +  ++ PIRV YH
Sbjct: 239 INRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYGTEPINTF----HGIQQNEDEPIRVSYH 298

Query: 316 GFGHYDALQIPATASKGV 334
              HY+++  P  A+ GV
Sbjct: 299 RNIHYNSVVNPNKATIGV 301

BLAST of MS023027 vs. ExPASy Swiss-Prot
Match: Q6GL44 (OTU domain-containing protein 5 OS=Xenopus tropicalis OX=8364 GN=otud5 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.4e-06
Identity = 37/138 (26.81%), Postives = 61/138 (44.20%), Query Frame = 0

Query: 196 DGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIKRRKETEWFVEGDFDRY 255
           DG CLFR+V           A      +D+ + +R    D  +K       +V  DF  Y
Sbjct: 179 DGACLFRAV-----------ADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTY 238

Query: 256 VSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEYGDEYGKENPIRVLYH 315
           ++  RK +  G   E+   + + + P+ VY Y  +         +G +  ++ PIRV YH
Sbjct: 239 INRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYGTEPINTF----HGIQQNEDEPIRVSYH 298

Query: 316 GFGHYDALQIPATASKGV 334
              HY+++  P  A+ GV
Sbjct: 299 RNIHYNSVVNPNKATIGV 301

BLAST of MS023027 vs. ExPASy TrEMBL
Match: A0A6J1DJP8 (OTU domain-containing protein At3g57810 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021529 PE=4 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.0e-194
Identity = 332/338 (98.22%), Postives = 332/338 (98.22%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           MNIGSISACTRNVVHLNSRVPMQMGSNICSVLS R SNSCCSYGISRPKYADQSVTTISS
Sbjct: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSPRISNSCCSYGISRPKYADQSVTTISS 60

Query: 61  CTPNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIP 120
           CTPNTSQRFP GCLSYCFSRQPIDFKNLTVKDLIT GGSHGRGLEISLACKGMNVKLSI 
Sbjct: 61  CTPNTSQRFPGGCLSYCFSRQPIDFKNLTVKDLITDGGSHGRGLEISLACKGMNVKLSIT 120

Query: 121 NDGFSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFSH 180
           NDGFSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFSH
Sbjct: 121 NDGFSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFSH 180

Query: 181 GKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIKR 240
           GKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGK APSESLQRDLADELRNKVADEFIKR
Sbjct: 181 GKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKAAPSESLQRDLADELRNKVADEFIKR 240

Query: 241 RKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEY 300
           RKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEY
Sbjct: 241 RKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEY 300

Query: 301 GDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           GDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL
Sbjct: 301 GDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 338

BLAST of MS023027 vs. ExPASy TrEMBL
Match: A0A1S3B6T1 (OTU domain-containing protein At3g57810 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486791 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.6e-168
Identity = 296/339 (87.32%), Postives = 311/339 (91.74%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           M+IGSIS CTRN V LN R+  QMGSNIC+VLSRRTS SCCSYGISRPKYADQSVTTISS
Sbjct: 1   MSIGSISICTRNAVPLNLRIYTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60

Query: 61  CTPNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIP 120
           C+PNTSQRF  GCLS CFSR+ IDF+  TVKDLIT GGS GR +EISLACKGMNVKLSIP
Sbjct: 61  CSPNTSQRFQGGCLSTCFSRRSIDFQAFTVKDLITDGGSPGREVEISLACKGMNVKLSIP 120

Query: 121 NDG-FSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFS 180
           NDG FSKIK+NMRWPER ASAGLVFGWVVCYS+SEPVHAEAA EK+DNEENSDSS VK S
Sbjct: 121 NDGTFSKIKYNMRWPERWASAGLVFGWVVCYSTSEPVHAEAAYEKDDNEENSDSSHVKLS 180

Query: 181 HGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIK 240
           HGK VYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELR+ VADEFIK
Sbjct: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRSNVADEFIK 240

Query: 241 RRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300
           RR+ETEWFVEGDFD YVSNMRKPH+WGGEPELFMASHVL APIIVYMYDKDSGGLISIAE
Sbjct: 241 RREETEWFVEGDFDTYVSNMRKPHVWGGEPELFMASHVLQAPIIVYMYDKDSGGLISIAE 300

Query: 301 YGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           YGDEYGKENPIRVLYHGFGHYDALQIP  A++GV RSKL
Sbjct: 301 YGDEYGKENPIRVLYHGFGHYDALQIP--ANQGVGRSKL 337

BLAST of MS023027 vs. ExPASy TrEMBL
Match: A0A5D3DP86 (OTU domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005670 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.6e-168
Identity = 296/339 (87.32%), Postives = 311/339 (91.74%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           M+IGSIS CTRN V LN R+  QMGSNIC+VLSRRTS SCCSYGISRPKYADQSVTTISS
Sbjct: 1   MSIGSISICTRNAVPLNLRIYTQMGSNICTVLSRRTSTSCCSYGISRPKYADQSVTTISS 60

Query: 61  CTPNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIP 120
           C+PNTSQRF  GCLS CFSR+ IDF+  TVKDLIT GGS GR +EISLACKGMNVKLSIP
Sbjct: 61  CSPNTSQRFQGGCLSTCFSRRSIDFQAFTVKDLITDGGSPGREVEISLACKGMNVKLSIP 120

Query: 121 NDG-FSKIKHNMRWPERCASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVKFS 180
           NDG FSKIK+NMRWPER ASAGLVFGWVVCYS+SEPVHAEAA EK+DNEENSDSS VK S
Sbjct: 121 NDGTFSKIKYNMRWPERWASAGLVFGWVVCYSTSEPVHAEAAYEKDDNEENSDSSHVKLS 180

Query: 181 HGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIK 240
           HGK VYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELR+ VADEFIK
Sbjct: 181 HGKKVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRSNVADEFIK 240

Query: 241 RRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAE 300
           RR+ETEWFVEGDFD YVSNMRKPH+WGGEPELFMASHVL APIIVYMYDKDSGGLISIAE
Sbjct: 241 RREETEWFVEGDFDTYVSNMRKPHVWGGEPELFMASHVLQAPIIVYMYDKDSGGLISIAE 300

Query: 301 YGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           YGDEYGKENPIRVLYHGFGHYDALQIP  A++GV RSKL
Sbjct: 301 YGDEYGKENPIRVLYHGFGHYDALQIP--ANQGVGRSKL 337

BLAST of MS023027 vs. ExPASy TrEMBL
Match: A0A6J1FB45 (OTU domain-containing protein At3g57810 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442443 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.6e-160
Identity = 282/341 (82.70%), Postives = 302/341 (88.56%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           M+IGSIS CTRNV+ LN RV  QMGSNIC+VLS RT  SCCSYGISRPKY DQSVT ISS
Sbjct: 1   MSIGSISVCTRNVIPLNFRVCTQMGSNICTVLSGRTPTSCCSYGISRPKYGDQSVTIISS 60

Query: 61  CTPNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIP 120
           CTPNT QR   GCLSYCFSR+P DF+ LTVKDLIT GGS GRGLEISLACKGMN K+S+P
Sbjct: 61  CTPNTCQRIQGGCLSYCFSRRPNDFEALTVKDLITDGGSRGRGLEISLACKGMNAKISVP 120

Query: 121 NDGF-SKIKHNMRWPERC--ASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVK 180
           +DG  SKIK+NMRWPERC  ASAGLVFGWVVC S+SEPVHAEAA +K+ NEEN+DSS VK
Sbjct: 121 SDGMCSKIKYNMRWPERCASASAGLVFGWVVCCSTSEPVHAEAAYDKKGNEENNDSSHVK 180

Query: 181 FSHGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEF 240
           FSHGK VY DYSVIGIPGDGRCLFRSVAHGACLRSGK AP+ESLQR+LADELR KVADEF
Sbjct: 181 FSHGKKVYIDYSVIGIPGDGRCLFRSVAHGACLRSGKSAPTESLQRELADELRTKVADEF 240

Query: 241 IKRRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 300
           IKRR+ETEWFVEGDFD YVSNMRKPHIWGGEPELFMASHVL APIIVYMYDKD+GGLISI
Sbjct: 241 IKRREETEWFVEGDFDTYVSNMRKPHIWGGEPELFMASHVLQAPIIVYMYDKDAGGLISI 300

Query: 301 AEYGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           AEYGDEYGK+NPI+VLYHGFGHYDALQIPA   +   RSKL
Sbjct: 301 AEYGDEYGKDNPIKVLYHGFGHYDALQIPANQGQ-AGRSKL 340

BLAST of MS023027 vs. ExPASy TrEMBL
Match: A0A6J1F5B0 (OTU domain-containing protein At3g57810 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442443 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.6e-160
Identity = 282/341 (82.70%), Postives = 302/341 (88.56%), Query Frame = 0

Query: 1   MNIGSISACTRNVVHLNSRVPMQMGSNICSVLSRRTSNSCCSYGISRPKYADQSVTTISS 60
           M+IGSIS CTRNV+ LN RV  QMGSNIC+VLS RT  SCCSYGISRPKY DQSVT ISS
Sbjct: 13  MSIGSISVCTRNVIPLNFRVCTQMGSNICTVLSGRTPTSCCSYGISRPKYGDQSVTIISS 72

Query: 61  CTPNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIP 120
           CTPNT QR   GCLSYCFSR+P DF+ LTVKDLIT GGS GRGLEISLACKGMN K+S+P
Sbjct: 73  CTPNTCQRIQGGCLSYCFSRRPNDFEALTVKDLITDGGSRGRGLEISLACKGMNAKISVP 132

Query: 121 NDGF-SKIKHNMRWPERC--ASAGLVFGWVVCYSSSEPVHAEAACEKEDNEENSDSSPVK 180
           +DG  SKIK+NMRWPERC  ASAGLVFGWVVC S+SEPVHAEAA +K+ NEEN+DSS VK
Sbjct: 133 SDGMCSKIKYNMRWPERCASASAGLVFGWVVCCSTSEPVHAEAAYDKKGNEENNDSSHVK 192

Query: 181 FSHGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEF 240
           FSHGK VY DYSVIGIPGDGRCLFRSVAHGACLRSGK AP+ESLQR+LADELR KVADEF
Sbjct: 193 FSHGKKVYIDYSVIGIPGDGRCLFRSVAHGACLRSGKSAPTESLQRELADELRTKVADEF 252

Query: 241 IKRRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISI 300
           IKRR+ETEWFVEGDFD YVSNMRKPHIWGGEPELFMASHVL APIIVYMYDKD+GGLISI
Sbjct: 253 IKRREETEWFVEGDFDTYVSNMRKPHIWGGEPELFMASHVLQAPIIVYMYDKDAGGLISI 312

Query: 301 AEYGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           AEYGDEYGK+NPI+VLYHGFGHYDALQIPA   +   RSKL
Sbjct: 313 AEYGDEYGKDNPIKVLYHGFGHYDALQIPANQGQ-AGRSKL 352

BLAST of MS023027 vs. TAIR 10
Match: AT3G57810.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 167/340 (49.12%), Postives = 212/340 (62.35%), Query Frame = 0

Query: 6   ISACTRNVVHLNSRVPMQMGSNICSVLSRRTSN-SCCSY--GISRPKYADQSVTTISSCT 65
           I+ C+RN +     +   +GS +  V++  +S  SC S   G+SR  Y    V    S +
Sbjct: 8   ITTCSRNAI----SIKRHLGSRLYGVVAHGSSKFSCYSLLSGLSRRHYTGFRV----SVS 67

Query: 66  PNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIPND 125
              S    +G        +P      TV        +    LE+S      N+K      
Sbjct: 68  NRPSSWHDKGLFGSVLINRP------TV--------APKEKLEVSFLSPEANMKC----- 127

Query: 126 GFSKIKHNMR---WPERCASAGLVFGWVVCYSS-SEPVHAEAACEKEDNEENSDSSPVKF 185
             SKI+ NMR      R A  G++   +VCYSS S+  +A+++ +K+ N  +  SS  KF
Sbjct: 128 --SKIESNMRNLYCYSRFAYTGVIVSLLVCYSSTSQSAYADSSRDKDANNVHHHSSDGKF 187

Query: 186 SHGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFI 245
            +GK VYTDYS+IGIPGDGRCLFRSVAHG CLRSGK AP E +QR+LADELR +VADEFI
Sbjct: 188 HNGKRVYTDYSIIGIPGDGRCLFRSVAHGFCLRSGKLAPGEKMQRELADELRTRVADEFI 247

Query: 246 KRRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIA 305
           +RR+ETEWFVEGDFD YV  +R PH+WGGEPELFMASHVL  PI VYM D  +GGLISIA
Sbjct: 248 QRRQETEWFVEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDDKAGGLISIA 307

Query: 306 EYGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           EYG EYGK++PIRVLYHGFGHYDAL +  + +  + +SKL
Sbjct: 308 EYGQEYGKDDPIRVLYHGFGHYDALLLHESKA-SIPKSKL 317

BLAST of MS023027 vs. TAIR 10
Match: AT3G57810.3 (Cysteine proteinases superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 167/340 (49.12%), Postives = 212/340 (62.35%), Query Frame = 0

Query: 6   ISACTRNVVHLNSRVPMQMGSNICSVLSRRTSN-SCCSY--GISRPKYADQSVTTISSCT 65
           I+ C+RN +     +   +GS +  V++  +S  SC S   G+SR  Y    V    S +
Sbjct: 8   ITTCSRNAI----SIKRHLGSRLYGVVAHGSSKFSCYSLLSGLSRRHYTGFRV----SVS 67

Query: 66  PNTSQRFPEGCLSYCFSRQPIDFKNLTVKDLITGGGSHGRGLEISLACKGMNVKLSIPND 125
              S    +G        +P      TV        +    LE+S      N+K      
Sbjct: 68  NRPSSWHDKGLFGSVLINRP------TV--------APKEKLEVSFLSPEANMKC----- 127

Query: 126 GFSKIKHNMR---WPERCASAGLVFGWVVCYSS-SEPVHAEAACEKEDNEENSDSSPVKF 185
             SKI+ NMR      R A  G++   +VCYSS S+  +A+++ +K+ N  +  SS  KF
Sbjct: 128 --SKIESNMRNLYCYSRFAYTGVIVSLLVCYSSTSQSAYADSSRDKDANNVHHHSSDGKF 187

Query: 186 SHGKTVYTDYSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFI 245
            +GK VYTDYS+IGIPGDGRCLFRSVAHG CLRSGK AP E +QR+LADELR +VADEFI
Sbjct: 188 HNGKRVYTDYSIIGIPGDGRCLFRSVAHGFCLRSGKLAPGEKMQRELADELRTRVADEFI 247

Query: 246 KRRKETEWFVEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIA 305
           +RR+ETEWFVEGDFD YV  +R PH+WGGEPELFMASHVL  PI VYM D  +GGLISIA
Sbjct: 248 QRRQETEWFVEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDDKAGGLISIA 307

Query: 306 EYGDEYGKENPIRVLYHGFGHYDALQIPATASKGVRRSKL 339
           EYG EYGK++PIRVLYHGFGHYDAL +  + +  + +SKL
Sbjct: 308 EYGQEYGKDDPIRVLYHGFGHYDALLLHESKA-SIPKSKL 317

BLAST of MS023027 vs. TAIR 10
Match: AT3G57810.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 275.4 bits (703), Expect = 6.1e-74
Identity = 133/202 (65.84%), Postives = 161/202 (79.70%), Query Frame = 0

Query: 138 ASAGLVFGWVVCYSS-SEPVHAEAACEKEDNEENSDSSPVKFSHGKTVYTDYSVIGIPGD 197
           A  G++   +VCYSS S+  +A+++ +K+ N  +  SS  KF +GK VYTDYS+IGIPGD
Sbjct: 74  AYTGVIVSLLVCYSSTSQSAYADSSRDKDANNVHHHSSDGKFHNGKRVYTDYSIIGIPGD 133

Query: 198 GRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIKRRKETEWFVEGDFDRYV 257
           GRCLFRSVAHG CLRSGK AP E +QR+LADELR +VADEFI+RR+ETEWFVEGDFD YV
Sbjct: 134 GRCLFRSVAHGFCLRSGKLAPGEKMQRELADELRTRVADEFIQRRQETEWFVEGDFDTYV 193

Query: 258 SNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSGGLISIAEYGDEYGKENPIRVLYHG 317
             +R PH+WGGEPELFMASHVL  PI VYM D  +GGLISIAEYG EYGK++PIRVLYHG
Sbjct: 194 RQIRDPHVWGGEPELFMASHVLQMPITVYMKDDKAGGLISIAEYGQEYGKDDPIRVLYHG 253

Query: 318 FGHYDALQIPATASKGVRRSKL 339
           FGHYDAL +  + +  + +SKL
Sbjct: 254 FGHYDALLLHESKA-SIPKSKL 274

BLAST of MS023027 vs. TAIR 10
Match: AT2G38025.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 84.0 bits (206), Expect = 2.6e-16
Identity = 56/160 (35.00%), Postives = 78/160 (48.75%), Query Frame = 0

Query: 188 YSVIGIPGDGRCLFRSVAHGACLRSGKPAPSESLQRDLADELRNKVADEFIKRRKETEWF 247
           Y+V  + GDGRCLFR++  G     G    +   +RD ADELR  V +      KE E +
Sbjct: 76  YAVDRVKGDGRCLFRALVKGMAFNKGITL-NPQRERDDADELRMAVKEVICNDPKEREKY 135

Query: 248 --------VEGDFDRYVSNMRKPHIWGGEPELFMASHVLHAPIIVYMYDKDSG------- 307
                   V+    R+   + +   WGGE EL + S +   PIIVY+ + + G       
Sbjct: 136 KEALVAITVDESLKRFCQRIGRHDFWGGESELLVLSKLCKQPIIVYIPEHEHGRGGGYGP 195

Query: 308 GLISIAEYGDEY------GK--ENPIRVLYHGFGHYDALQ 325
           G I I EYG E+      GK  +N +R+LY G  HYD L+
Sbjct: 196 GFIPIQEYGSEFRGGWGKGKTNKNVVRLLYSGRNHYDLLR 234