MS022976 (gene) Bitter gourd (TR) v1

Overview
NameMS022976
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionConcanavalin A-like lectin protein kinase family protein
Locationscaffold357: 259667 .. 260590 (-)
RNA-Seq ExpressionMS022976
SyntenyMS022976
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCGAATTTCCAGAGCTGAAGAAGGCAACTGGCAATTTCGACCCCAAGAACAGGCTCGGGAGAGGCGGATTTGGAACGGTTTACAGAGGAAATTTGATGAACAGAGATGTCGCCGTGAAGAGAATCTCAGAGAATTCCCGTCAAGGGAAGCAAGAATTCATCGCGGAAGTGGCAACAATCGGTGGCCTCCATCACAAGAACCTCGTGAAACTAATGGGATGGTGCTACAAAAAGAGAGATCTTTTGTTGATATACGAATACATGCCCAACGGCAGCTTGGACAAGCTGATCTTCAGCAGCCATGACAAATCCTCCCCAACACCCAATTGGGAAATCAGACGGAACATAATCTGCGGAGTAGCGGAGGCTCTGGATTATCTTCACAACGGGTGCGAGAAGAGAGTGCTACACAGAGACGTGAAGCCTTCAAACATAATGCTGGATTCGAAGTTGGAAGCCAAATTGGGTGACTTCGGGTTGGCTCGGGCAATTCGTCGAACAGAACAGACGCATCACTCAACAAGAGGTATAGCTGGTACATACGGCTACATGGCACCAGAAATTTTCCTAACCCGCAGAGCAACAGCAGAGACAGACGTATACGCATTCGGGATTCTTGTTTTGGAAGTCGTGTGCGGGAGAAAGCGTACAAGTAAAAATCCGTCGAGTGGGTACGAAGGCCATATTACAAATTGGGTATGGGAGTTTCAAGAGAGAGGAGAGATTGTCGAAGCTGGTGATGGGAGAATTGGAGGGCAACACGAGGAAGAAGAGATGGAGAGAGCTTTGATTGTAGGATTGGCTTGTTGCCATCCGAACCCCCATGAGAGGCCGACCATGAAAAATGTTCTGAAAGTGCTTAAAGGGGAAGCAGATCCACCGATTCTGCCGCATGAAATGCCTTCGTTTGTGTGGCCGCTGGTG

mRNA sequence

TTCGAATTTCCAGAGCTGAAGAAGGCAACTGGCAATTTCGACCCCAAGAACAGGCTCGGGAGAGGCGGATTTGGAACGGTTTACAGAGGAAATTTGATGAACAGAGATGTCGCCGTGAAGAGAATCTCAGAGAATTCCCGTCAAGGGAAGCAAGAATTCATCGCGGAAGTGGCAACAATCGGTGGCCTCCATCACAAGAACCTCGTGAAACTAATGGGATGGTGCTACAAAAAGAGAGATCTTTTGTTGATATACGAATACATGCCCAACGGCAGCTTGGACAAGCTGATCTTCAGCAGCCATGACAAATCCTCCCCAACACCCAATTGGGAAATCAGACGGAACATAATCTGCGGAGTAGCGGAGGCTCTGGATTATCTTCACAACGGGTGCGAGAAGAGAGTGCTACACAGAGACGTGAAGCCTTCAAACATAATGCTGGATTCGAAGTTGGAAGCCAAATTGGGTGACTTCGGGTTGGCTCGGGCAATTCGTCGAACAGAACAGACGCATCACTCAACAAGAGGTATAGCTGGTACATACGGCTACATGGCACCAGAAATTTTCCTAACCCGCAGAGCAACAGCAGAGACAGACGTATACGCATTCGGGATTCTTGTTTTGGAAGTCGTGTGCGGGAGAAAGCGTACAAGTAAAAATCCGTCGAGTGGGTACGAAGGCCATATTACAAATTGGGTATGGGAGTTTCAAGAGAGAGGAGAGATTGTCGAAGCTGGTGATGGGAGAATTGGAGGGCAACACGAGGAAGAAGAGATGGAGAGAGCTTTGATTGTAGGATTGGCTTGTTGCCATCCGAACCCCCATGAGAGGCCGACCATGAAAAATGTTCTGAAAGTGCTTAAAGGGGAAGCAGATCCACCGATTCTGCCGCATGAAATGCCTTCGTTTGTGTGGCCGCTGGTG

Coding sequence (CDS)

TTCGAATTTCCAGAGCTGAAGAAGGCAACTGGCAATTTCGACCCCAAGAACAGGCTCGGGAGAGGCGGATTTGGAACGGTTTACAGAGGAAATTTGATGAACAGAGATGTCGCCGTGAAGAGAATCTCAGAGAATTCCCGTCAAGGGAAGCAAGAATTCATCGCGGAAGTGGCAACAATCGGTGGCCTCCATCACAAGAACCTCGTGAAACTAATGGGATGGTGCTACAAAAAGAGAGATCTTTTGTTGATATACGAATACATGCCCAACGGCAGCTTGGACAAGCTGATCTTCAGCAGCCATGACAAATCCTCCCCAACACCCAATTGGGAAATCAGACGGAACATAATCTGCGGAGTAGCGGAGGCTCTGGATTATCTTCACAACGGGTGCGAGAAGAGAGTGCTACACAGAGACGTGAAGCCTTCAAACATAATGCTGGATTCGAAGTTGGAAGCCAAATTGGGTGACTTCGGGTTGGCTCGGGCAATTCGTCGAACAGAACAGACGCATCACTCAACAAGAGGTATAGCTGGTACATACGGCTACATGGCACCAGAAATTTTCCTAACCCGCAGAGCAACAGCAGAGACAGACGTATACGCATTCGGGATTCTTGTTTTGGAAGTCGTGTGCGGGAGAAAGCGTACAAGTAAAAATCCGTCGAGTGGGTACGAAGGCCATATTACAAATTGGGTATGGGAGTTTCAAGAGAGAGGAGAGATTGTCGAAGCTGGTGATGGGAGAATTGGAGGGCAACACGAGGAAGAAGAGATGGAGAGAGCTTTGATTGTAGGATTGGCTTGTTGCCATCCGAACCCCCATGAGAGGCCGACCATGAAAAATGTTCTGAAAGTGCTTAAAGGGGAAGCAGATCCACCGATTCTGCCGCATGAAATGCCTTCGTTTGTGTGGCCGCTGGTG

Protein sequence

FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATIGGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQERGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPHEMPSFVWPLV
Homology
BLAST of MS022976 vs. NCBI nr
Match: XP_022151362.1 (probable L-type lectin-domain containing receptor kinase S.5 [Momordica charantia])

HSP 1 Score: 643.3 bits (1658), Expect = 1.1e-180
Identity = 305/308 (99.03%), Postives = 307/308 (99.68%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEF+AEVATI
Sbjct: 323 FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFVAEVATI 382

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 120
           GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV
Sbjct: 383 GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 442

Query: 121 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGT 180
           AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRG+AGT
Sbjct: 443 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGLAGT 502

Query: 181 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQERG 240
           YGYMAPEIFLTRRATAETDVYAFGILVLEVVC RKRTSKNPSSGYEGHITNWVWEFQERG
Sbjct: 503 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCRRKRTSKNPSSGYEGHITNWVWEFQERG 562

Query: 241 EIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPHEM 300
           EIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPHEM
Sbjct: 563 EIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPHEM 622

Query: 301 PSFVWPLV 309
           PSFVWPLV
Sbjct: 623 PSFVWPLV 630

BLAST of MS022976 vs. NCBI nr
Match: XP_022151409.1 (probable L-type lectin-domain containing receptor kinase S.5 [Momordica charantia])

HSP 1 Score: 590.5 bits (1521), Expect = 8.4e-165
Identity = 285/309 (92.23%), Postives = 289/309 (93.53%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           FEF ELKKATGNFD KNRLGRGGFGTVYRG+LMNR+VAVKRISENS QGKQEFIAEVATI
Sbjct: 329 FEFSELKKATGNFDAKNRLGRGGFGTVYRGDLMNREVAVKRISENSHQGKQEFIAEVATI 388

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 120
           GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIF   D SS TPNWE RRNIICGV
Sbjct: 389 GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFGGDDNSSATPNWETRRNIICGV 448

Query: 121 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGT 180
           AEALDYLHNGCEKRV+HRDVKPSNIMLDSKLEAKLGDFGLARAI RTEQTHHSTRGIAGT
Sbjct: 449 AEALDYLHNGCEKRVIHRDVKPSNIMLDSKLEAKLGDFGLARAIHRTEQTHHSTRGIAGT 508

Query: 181 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPS--SGYEGHITNWVWEFQE 240
           YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPS  S YEGHITNWVWEFQE
Sbjct: 509 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSNLSVYEGHITNWVWEFQE 568

Query: 241 RGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPH 300
           RGEI  A DGRIGGQ EEEEMER LIVGLACCHPNPHERPTMKNVL+VLKGEADPP LPH
Sbjct: 569 RGEIRGAADGRIGGQCEEEEMERTLIVGLACCHPNPHERPTMKNVLQVLKGEADPPXLPH 628

Query: 301 EMPSFVWPL 308
           EMP FVWPL
Sbjct: 629 EMPLFVWPL 637

BLAST of MS022976 vs. NCBI nr
Match: XP_022158345.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111024852 [Momordica charantia])

HSP 1 Score: 512.3 bits (1318), Expect = 2.9e-141
Identity = 247/308 (80.19%), Postives = 269/308 (87.34%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           F F ELK ATGNFDPKNRLGRGGFGTVYRGNLMN+DVAVKRISE+SRQGKQEFIAEVATI
Sbjct: 331 FGFTELKNATGNFDPKNRLGRGGFGTVYRGNLMNKDVAVKRISEDSRQGKQEFIAEVATI 390

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 120
           GGLHHKNLVKL+GWCY+KRDLLL+YEYMPNGSLDKLIF   DKSS  PNWEIR+NII GV
Sbjct: 391 GGLHHKNLVKLIGWCYQKRDLLLVYEYMPNGSLDKLIFGGDDKSSTAPNWEIRQNIIYGV 450

Query: 121 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGT 180
           AEALDYLHNGCEK VLHRDVK SNIMLDS+ EAKLGDFGLAR IRRTEQTHHSTR IAGT
Sbjct: 451 AEALDYLHNGCEKTVLHRDVKSSNIMLDSRFEAKLGDFGLARTIRRTEQTHHSTREIAGT 510

Query: 181 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQERG 240
            GYMAPEIFLT RATAETDVYAFG+LVLEVVCGRK  S +   GY+G + +W WEF + G
Sbjct: 511 PGYMAPEIFLTSRATAETDVYAFGVLVLEVVCGRKPGSPSELGGYDGSLAHWAWEFHKEG 570

Query: 241 EIVEAGDGRI--GGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPH 300
           +I EA D RI   G+  +EEME  LI+GLACCHPNP +RPTM+NVL+VLKGEA+PPILP+
Sbjct: 571 KIGEAVDERIEVEGEFVKEEMEYLLILGLACCHPNPLQRPTMRNVLQVLKGEANPPILPN 630

Query: 301 EMPSFVWP 307
           E PSFVWP
Sbjct: 631 ERPSFVWP 638

BLAST of MS022976 vs. NCBI nr
Match: XP_023527734.1 (probable L-type lectin-domain containing receptor kinase S.5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503.8 bits (1296), Expect = 1.0e-138
Identity = 241/315 (76.51%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           F F ELKKAT NFDPKNRLGRGGFGTVY+GNL NR+VAVKRISE+SRQGKQEFI EVATI
Sbjct: 336 FRFRELKKATDNFDPKNRLGRGGFGTVYKGNLTNREVAVKRISEDSRQGKQEFITEVATI 395

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSS--------HDKSSPTPNWEI 120
           GGLHHKNLVKL+GWCY+KRD LL+YEYMPNGSLDKLIF           ++ S  PNWEI
Sbjct: 396 GGLHHKNLVKLIGWCYEKRDFLLVYEYMPNGSLDKLIFGGGGGDSGYVDNRFSTGPNWEI 455

Query: 121 RRNIICGVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHH 180
           R +II GVAEALDYLHNGCEK VLHRDVK SNIMLDSK E KLGDFGLAR IRRTEQTHH
Sbjct: 456 RLSIIYGVAEALDYLHNGCEKTVLHRDVKSSNIMLDSKFEPKLGDFGLARTIRRTEQTHH 515

Query: 181 STRGIAGTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSG-YEGHITN 240
           STR IAGT GYMAPEIFLT RAT ETDVYAFG+LVLEV CGRK  + +   G Y+G IT+
Sbjct: 516 STREIAGTRGYMAPEIFLTSRATVETDVYAFGVLVLEVACGRKAANPSEIGGNYDGDITH 575

Query: 241 WVWEFQERGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEA 300
           WVWEFQERGEIV+A D R+ GQ+E+EEMER L++GL CCHPNPH+RPTMKNVL+VL GE 
Sbjct: 576 WVWEFQERGEIVKAADERMAGQYEKEEMERILVLGLGCCHPNPHQRPTMKNVLQVLTGEM 635

Query: 301 DPPILPHEMPSFVWP 307
           +PP LPHE P+F+WP
Sbjct: 636 NPPTLPHEKPTFMWP 650

BLAST of MS022976 vs. NCBI nr
Match: XP_022935657.1 (probable L-type lectin-domain containing receptor kinase S.5 [Cucurbita moschata])

HSP 1 Score: 502.7 bits (1293), Expect = 2.3e-138
Identity = 241/315 (76.51%), Postives = 265/315 (84.13%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           F F ELKKAT NFDPKNRLGRGGFGTVY+GNL NR+VAVKRISE+SRQGKQEFI EVATI
Sbjct: 336 FRFRELKKATDNFDPKNRLGRGGFGTVYKGNLTNREVAVKRISEDSRQGKQEFITEVATI 395

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHD--------KSSPTPNWEI 120
           GGLHHKNLVKL+GWCY+KRD LL+YEYMPNGSLDKLIF            + S  PNWEI
Sbjct: 396 GGLHHKNLVKLIGWCYEKRDFLLVYEYMPNGSLDKLIFGGGGGDSGYVDYRFSTGPNWEI 455

Query: 121 RRNIICGVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHH 180
           R +II GVAEALDYLHNGCEK VLHRDVK SNIMLDSK E KLGDFGLAR IRRTEQTHH
Sbjct: 456 RLSIIYGVAEALDYLHNGCEKTVLHRDVKSSNIMLDSKFEPKLGDFGLARTIRRTEQTHH 515

Query: 181 STRGIAGTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSG-YEGHITN 240
           STR IAGT GYMAPEIFLT RAT ETDVYAFG+LVLEV CGRK  + +   G Y+G IT+
Sbjct: 516 STREIAGTRGYMAPEIFLTSRATVETDVYAFGVLVLEVACGRKAANPSEIGGNYDGDITH 575

Query: 241 WVWEFQERGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEA 300
           WVWEFQERGEIV+A D R+ GQ+E+EEMER L++GL CCHPNPH+RPTMKNVL+VL GE 
Sbjct: 576 WVWEFQERGEIVKAADERMAGQYEKEEMERILVLGLGCCHPNPHQRPTMKNVLQVLTGEM 635

Query: 301 DPPILPHEMPSFVWP 307
           +PP LPHE P+F+WP
Sbjct: 636 NPPTLPHEKPTFMWP 650

BLAST of MS022976 vs. ExPASy Swiss-Prot
Match: Q9FG33 (Probable L-type lectin-domain containing receptor kinase S.5 OS=Arabidopsis thaliana OX=3702 GN=LECRKS5 PE=2 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.0e-113
Identity = 188/309 (60.84%), Postives = 238/309 (77.02%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           F+  ELK+ATGNF  +N+LG+GGFG V++G    RD+AVKR+SE S QGKQEFIAE+ TI
Sbjct: 318 FKLRELKRATGNFGAENKLGQGGFGMVFKGKWQGRDIAVKRVSEKSHQGKQEFIAEITTI 377

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 120
           G L+H+NLVKL+GWCY++++ LL+YEYMPNGSLDK +F   DKS     WE R+NII G+
Sbjct: 378 GNLNHRNLVKLLGWCYERKEYLLVYEYMPNGSLDKYLF-LEDKSRSNLTWETRKNIITGL 437

Query: 121 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGT 180
           ++AL+YLHNGCEKR+LHRD+K SN+MLDS   AKLGDFGLAR I+++E THHST+ IAGT
Sbjct: 438 SQALEYLHNGCEKRILHRDIKASNVMLDSDFNAKLGDFGLARMIQQSEMTHHSTKEIAGT 497

Query: 181 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRT---SKNPSSGYEGHITNWVWEFQ 240
            GYMAPE FL  RAT ETDVYAFG+L+LEVV G+K +    K+  + Y   I NW+WE  
Sbjct: 498 PGYMAPETFLNGRATVETDVYAFGVLMLEVVSGKKPSYVLVKDNQNNYNNSIVNWLWELY 557

Query: 241 ERGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILP 300
             G I +A D  +G   ++EEM+  L++GLACCHPNP++RP+MK VLKVL GE  PP +P
Sbjct: 558 RNGTITDAADPGMGNLFDKEEMKSVLLLGLACCHPNPNQRPSMKTVLKVLTGETSPPDVP 617

Query: 301 HEMPSFVWP 307
            E P+FVWP
Sbjct: 618 TERPAFVWP 625

BLAST of MS022976 vs. ExPASy Swiss-Prot
Match: Q9LFH9 (L-type lectin-domain containing receptor kinase VIII.1 OS=Arabidopsis thaliana OX=3702 GN=LECRK81 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.2e-78
Identity = 148/309 (47.90%), Postives = 201/309 (65.05%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRD--VAVKRISENSRQGKQEFIAEVA 60
           F + ELK  T NF+    +G G FG VYRG L      VAVKR S +S+  K EF++E++
Sbjct: 364 FSYKELKAGTKNFNESRIIGHGAFGVVYRGILPETGDIVAVKRCSHSSQDKKNEFLSELS 423

Query: 61  TIGGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIIC 120
            IG L H+NLV+L GWC++K ++LL+Y+ MPNGSLDK +F S      T  W+ R+ I+ 
Sbjct: 424 IIGSLRHRNLVRLQGWCHEKGEILLVYDLMPNGSLDKALFESRF----TLPWDHRKKILL 483

Query: 121 GVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIA 180
           GVA AL YLH  CE +V+HRDVK SNIMLD    AKLGDFGLAR I   +    +    A
Sbjct: 484 GVASALAYLHRECENQVIHRDVKSSNIMLDESFNAKLGDFGLARQIEHDKSPEATV--AA 543

Query: 181 GTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKN-----PSSGYEGHITNWV 240
           GT GY+APE  LT RA+ +TDV+++G +VLEVV GR+   K+      + G   ++  WV
Sbjct: 544 GTMGYLAPEYLLTGRASEKTDVFSYGAVVLEVVSGRRPIEKDLNVQRHNVGVNPNLVEWV 603

Query: 241 WEFQERGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADP 300
           W   + G++  A D R+ G+ +E EM R L+VGLAC HP+P  RPTM++V+++L GEAD 
Sbjct: 604 WGLYKEGKVSAAADSRLEGKFDEGEMWRVLVVGLACSHPDPAFRPTMRSVVQMLIGEADV 663

Query: 301 PILPHEMPS 303
           P++P   P+
Sbjct: 664 PVVPKSRPT 666

BLAST of MS022976 vs. ExPASy Swiss-Prot
Match: Q9FHG4 (Probable L-type lectin-domain containing receptor kinase S.7 OS=Arabidopsis thaliana OX=3702 GN=LECRKS7 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.6e-76
Identity = 138/304 (45.39%), Postives = 196/304 (64.47%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDV--AVKRISENSRQGKQEFIAEVA 60
           F + EL  AT  F     +GRG FG VYR   ++     AVKR   NS +GK EF+AE++
Sbjct: 353 FSYKELYTATKGFHSSRVIGRGAFGNVYRAMFVSSGTISAVKRSRHNSTEGKTEFLAELS 412

Query: 61  TIGGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIIC 120
            I  L HKNLV+L GWC +K +LLL+YE+MPNGSLDK+++      +   +W  R NI  
Sbjct: 413 IIACLRHKNLVQLQGWCNEKGELLLVYEFMPNGSLDKILYQESQTGAVALDWSHRLNIAI 472

Query: 121 GVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIA 180
           G+A AL YLH+ CE++V+HRD+K SNIMLD    A+LGDFGLAR +   +++  ST   A
Sbjct: 473 GLASALSYLHHECEQQVVHRDIKTSNIMLDINFNARLGDFGLAR-LTEHDKSPVSTL-TA 532

Query: 181 GTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQE 240
           GT GY+APE      AT +TD +++G+++LEV CGR+   K P S    ++ +WVW    
Sbjct: 533 GTMGYLAPEYLQYGTATEKTDAFSYGVVILEVACGRRPIDKEPESQKTVNLVDWVWRLHS 592

Query: 241 RGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPH 300
            G ++EA D R+ G+ +EE M++ L+VGL C HP+ +ERP+M+ VL++L  E +P  +P 
Sbjct: 593 EGRVLEAVDERLKGEFDEEMMKKLLLVGLKCAHPDSNERPSMRRVLQILNNEIEPSPVPK 652

Query: 301 EMPS 303
             P+
Sbjct: 653 MKPT 654

BLAST of MS022976 vs. ExPASy Swiss-Prot
Match: Q9M021 (L-type lectin-domain containing receptor kinase VI.2 OS=Arabidopsis thaliana OX=3702 GN=LECRK62 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.7e-73
Identity = 140/298 (46.98%), Postives = 188/298 (63.09%), Query Frame = 0

Query: 3   FPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRD-VAVKRISENSRQGKQEFIAEVATIG 62
           + +L  AT  F     +G GGFGTV++G L N D +AVK+I  +SRQG +EF+AE+ ++G
Sbjct: 357 YRDLYVATDGFKKTGIIGTGGFGTVFKGKLPNSDPIAVKKIIPSSRQGVREFVAEIESLG 416

Query: 63  GLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGVA 122
            L HKNLV L GWC  K DLLLIY+Y+PNGSLD L+++   +S    +W  R  I  G+A
Sbjct: 417 KLRHKNLVNLQGWCKHKNDLLLIYDYIPNGSLDSLLYTVPRRSGAVLSWNARFQIAKGIA 476

Query: 123 EALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGTY 182
             L YLH   EK V+HRDVKPSN+++DSK+  +LGDFGLAR   R   T   T  + GT 
Sbjct: 477 SGLLYLHEEWEKIVIHRDVKPSNVLIDSKMNPRLGDFGLARLYER--GTLSETTALVGTI 536

Query: 183 GYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQERGE 242
           GYMAPE+      ++ +DV+AFG+L+LE+VCGRK     P+      + +WV E    GE
Sbjct: 537 GYMAPELSRNGNPSSASDVFAFGVLLLEIVCGRK-----PTDSGTFFLVDWVMELHANGE 596

Query: 243 IVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPHE 300
           I+ A D R+G  ++  E   AL VGL CCH  P  RP+M+ VL+ L GE + P +  E
Sbjct: 597 ILSAIDPRLGSGYDGGEARLALAVGLLCCHQKPASRPSMRIVLRYLNGEENVPEIDDE 647

BLAST of MS022976 vs. ExPASy Swiss-Prot
Match: Q66GN2 (Lectin-domain containing receptor kinase VI.4 OS=Arabidopsis thaliana OX=3702 GN=LECRK64 PE=2 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 3.5e-73
Identity = 139/296 (46.96%), Postives = 187/296 (63.18%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNR--DVAVKRISENSRQGKQEFIAEVA 60
           F + +L KAT  F     +G GGFG VYRGN+ +    +AVK+I+ NS QG +EF+AE+ 
Sbjct: 351 FRYRDLYKATEGFKENRVVGTGGFGIVYRGNIRSSSDQIAVKKITPNSMQGVREFVAEIE 410

Query: 61  TIGGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIIC 120
           ++G L HKNLV L GWC  + DLLLIY+Y+PNGSLD L++S   +S    +W  R  I  
Sbjct: 411 SLGRLRHKNLVNLQGWCKHRNDLLLIYDYIPNGSLDSLLYSKPRRSGAVLSWNARFQIAK 470

Query: 121 GVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIA 180
           G+A  L YLH   E+ V+HRDVKPSN+++DS +  +LGDFGLAR   R  Q+   T  + 
Sbjct: 471 GIASGLLYLHEEWEQIVIHRDVKPSNVLIDSDMNPRLGDFGLARLYERGSQS--CTTVVV 530

Query: 181 GTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQE 240
           GT GYMAPE+     +++ +DV+AFG+L+LE+V GRK     P+      I +WV E Q 
Sbjct: 531 GTIGYMAPELARNGNSSSASDVFAFGVLLLEIVSGRK-----PTDSGTFFIADWVMELQA 590

Query: 241 RGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPP 295
            GEI+ A D R+G  ++E E   AL VGL CCH  P  RP M+ VL+ L  + D P
Sbjct: 591 SGEILSAIDPRLGSGYDEGEARLALAVGLLCCHHKPESRPLMRMVLRYLNRDEDVP 639

BLAST of MS022976 vs. ExPASy TrEMBL
Match: A0A6J1DDA1 (probable L-type lectin-domain containing receptor kinase S.5 OS=Momordica charantia OX=3673 GN=LOC111019323 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 5.3e-181
Identity = 305/308 (99.03%), Postives = 307/308 (99.68%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEF+AEVATI
Sbjct: 323 FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFVAEVATI 382

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 120
           GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV
Sbjct: 383 GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 442

Query: 121 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGT 180
           AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRG+AGT
Sbjct: 443 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGLAGT 502

Query: 181 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQERG 240
           YGYMAPEIFLTRRATAETDVYAFGILVLEVVC RKRTSKNPSSGYEGHITNWVWEFQERG
Sbjct: 503 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCRRKRTSKNPSSGYEGHITNWVWEFQERG 562

Query: 241 EIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPHEM 300
           EIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPHEM
Sbjct: 563 EIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPHEM 622

Query: 301 PSFVWPLV 309
           PSFVWPLV
Sbjct: 623 PSFVWPLV 630

BLAST of MS022976 vs. ExPASy TrEMBL
Match: A0A6J1DD00 (probable L-type lectin-domain containing receptor kinase S.5 OS=Momordica charantia OX=3673 GN=LOC111019347 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.1e-165
Identity = 285/309 (92.23%), Postives = 289/309 (93.53%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           FEF ELKKATGNFD KNRLGRGGFGTVYRG+LMNR+VAVKRISENS QGKQEFIAEVATI
Sbjct: 329 FEFSELKKATGNFDAKNRLGRGGFGTVYRGDLMNREVAVKRISENSHQGKQEFIAEVATI 388

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 120
           GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIF   D SS TPNWE RRNIICGV
Sbjct: 389 GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFGGDDNSSATPNWETRRNIICGV 448

Query: 121 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGT 180
           AEALDYLHNGCEKRV+HRDVKPSNIMLDSKLEAKLGDFGLARAI RTEQTHHSTRGIAGT
Sbjct: 449 AEALDYLHNGCEKRVIHRDVKPSNIMLDSKLEAKLGDFGLARAIHRTEQTHHSTRGIAGT 508

Query: 181 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPS--SGYEGHITNWVWEFQE 240
           YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPS  S YEGHITNWVWEFQE
Sbjct: 509 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSNLSVYEGHITNWVWEFQE 568

Query: 241 RGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPH 300
           RGEI  A DGRIGGQ EEEEMER LIVGLACCHPNPHERPTMKNVL+VLKGEADPP LPH
Sbjct: 569 RGEIRGAADGRIGGQCEEEEMERTLIVGLACCHPNPHERPTMKNVLQVLKGEADPPXLPH 628

Query: 301 EMPSFVWPL 308
           EMP FVWPL
Sbjct: 629 EMPLFVWPL 637

BLAST of MS022976 vs. ExPASy TrEMBL
Match: A0A6J1DVK5 (LOW QUALITY PROTEIN: uncharacterized protein LOC111024852 OS=Momordica charantia OX=3673 GN=LOC111024852 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.4e-141
Identity = 247/308 (80.19%), Postives = 269/308 (87.34%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           F F ELK ATGNFDPKNRLGRGGFGTVYRGNLMN+DVAVKRISE+SRQGKQEFIAEVATI
Sbjct: 331 FGFTELKNATGNFDPKNRLGRGGFGTVYRGNLMNKDVAVKRISEDSRQGKQEFIAEVATI 390

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 120
           GGLHHKNLVKL+GWCY+KRDLLL+YEYMPNGSLDKLIF   DKSS  PNWEIR+NII GV
Sbjct: 391 GGLHHKNLVKLIGWCYQKRDLLLVYEYMPNGSLDKLIFGGDDKSSTAPNWEIRQNIIYGV 450

Query: 121 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGT 180
           AEALDYLHNGCEK VLHRDVK SNIMLDS+ EAKLGDFGLAR IRRTEQTHHSTR IAGT
Sbjct: 451 AEALDYLHNGCEKTVLHRDVKSSNIMLDSRFEAKLGDFGLARTIRRTEQTHHSTREIAGT 510

Query: 181 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQERG 240
            GYMAPEIFLT RATAETDVYAFG+LVLEVVCGRK  S +   GY+G + +W WEF + G
Sbjct: 511 PGYMAPEIFLTSRATAETDVYAFGVLVLEVVCGRKPGSPSELGGYDGSLAHWAWEFHKEG 570

Query: 241 EIVEAGDGRI--GGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPH 300
           +I EA D RI   G+  +EEME  LI+GLACCHPNP +RPTM+NVL+VLKGEA+PPILP+
Sbjct: 571 KIGEAVDERIEVEGEFVKEEMEYLLILGLACCHPNPLQRPTMRNVLQVLKGEANPPILPN 630

Query: 301 EMPSFVWP 307
           E PSFVWP
Sbjct: 631 ERPSFVWP 638

BLAST of MS022976 vs. ExPASy TrEMBL
Match: A0A6J1F671 (probable L-type lectin-domain containing receptor kinase S.5 OS=Cucurbita moschata OX=3662 GN=LOC111442448 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.1e-138
Identity = 241/315 (76.51%), Postives = 265/315 (84.13%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           F F ELKKAT NFDPKNRLGRGGFGTVY+GNL NR+VAVKRISE+SRQGKQEFI EVATI
Sbjct: 336 FRFRELKKATDNFDPKNRLGRGGFGTVYKGNLTNREVAVKRISEDSRQGKQEFITEVATI 395

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHD--------KSSPTPNWEI 120
           GGLHHKNLVKL+GWCY+KRD LL+YEYMPNGSLDKLIF            + S  PNWEI
Sbjct: 396 GGLHHKNLVKLIGWCYEKRDFLLVYEYMPNGSLDKLIFGGGGGDSGYVDYRFSTGPNWEI 455

Query: 121 RRNIICGVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHH 180
           R +II GVAEALDYLHNGCEK VLHRDVK SNIMLDSK E KLGDFGLAR IRRTEQTHH
Sbjct: 456 RLSIIYGVAEALDYLHNGCEKTVLHRDVKSSNIMLDSKFEPKLGDFGLARTIRRTEQTHH 515

Query: 181 STRGIAGTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSG-YEGHITN 240
           STR IAGT GYMAPEIFLT RAT ETDVYAFG+LVLEV CGRK  + +   G Y+G IT+
Sbjct: 516 STREIAGTRGYMAPEIFLTSRATVETDVYAFGVLVLEVACGRKAANPSEIGGNYDGDITH 575

Query: 241 WVWEFQERGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEA 300
           WVWEFQERGEIV+A D R+ GQ+E+EEMER L++GL CCHPNPH+RPTMKNVL+VL GE 
Sbjct: 576 WVWEFQERGEIVKAADERMAGQYEKEEMERILVLGLGCCHPNPHQRPTMKNVLQVLTGEM 635

Query: 301 DPPILPHEMPSFVWP 307
           +PP LPHE P+F+WP
Sbjct: 636 NPPTLPHEKPTFMWP 650

BLAST of MS022976 vs. ExPASy TrEMBL
Match: A0A6J1J0L6 (probable L-type lectin-domain containing receptor kinase S.5 OS=Cucurbita maxima OX=3661 GN=LOC111481592 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.5e-138
Identity = 240/314 (76.43%), Postives = 265/314 (84.39%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           F F ELKKAT NFDPKNRLGRGGFGTVY+GNL NR+VAVKRISE+SRQGKQEFI EVATI
Sbjct: 336 FRFRELKKATDNFDPKNRLGRGGFGTVYKGNLTNREVAVKRISEDSRQGKQEFITEVATI 395

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSS-------HDKSSPTPNWEIR 120
           GGLHHKNLVKL+GWCY+KRD LL+YEYMPNGSLDKLIF          ++ S   NWEIR
Sbjct: 396 GGLHHKNLVKLIGWCYEKRDFLLVYEYMPNGSLDKLIFGGGGDGGYVDNRFSTGLNWEIR 455

Query: 121 RNIICGVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHS 180
            +II GVAEALDYLHNGCEK VLHRDVK SNIMLDSK E KLGDFGLAR IRRTEQTHHS
Sbjct: 456 LSIIYGVAEALDYLHNGCEKTVLHRDVKSSNIMLDSKFEPKLGDFGLARTIRRTEQTHHS 515

Query: 181 TRGIAGTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSG-YEGHITNW 240
           TR IAGT GYMAPEIFLT RAT ETDVYAFG+LVLEV CGRK  + +   G Y+G IT+W
Sbjct: 516 TREIAGTRGYMAPEIFLTSRATVETDVYAFGVLVLEVACGRKAANPSEIGGNYDGDITHW 575

Query: 241 VWEFQERGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEAD 300
           VWEFQERGEIV+A D R+ GQ+E+EEMER L++GL CCHPNPH+RPTMKNVL+VL GE +
Sbjct: 576 VWEFQERGEIVKAADERMAGQYEKEEMERILVLGLGCCHPNPHQRPTMKNVLQVLTGEMN 635

Query: 301 PPILPHEMPSFVWP 307
           PP LPHE P+F+WP
Sbjct: 636 PPTLPHEKPTFMWP 649

BLAST of MS022976 vs. TAIR 10
Match: AT5G06740.1 (Concanavalin A-like lectin protein kinase family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.4e-114
Identity = 188/309 (60.84%), Postives = 238/309 (77.02%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDVAVKRISENSRQGKQEFIAEVATI 60
           F+  ELK+ATGNF  +N+LG+GGFG V++G    RD+AVKR+SE S QGKQEFIAE+ TI
Sbjct: 318 FKLRELKRATGNFGAENKLGQGGFGMVFKGKWQGRDIAVKRVSEKSHQGKQEFIAEITTI 377

Query: 61  GGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGV 120
           G L+H+NLVKL+GWCY++++ LL+YEYMPNGSLDK +F   DKS     WE R+NII G+
Sbjct: 378 GNLNHRNLVKLLGWCYERKEYLLVYEYMPNGSLDKYLF-LEDKSRSNLTWETRKNIITGL 437

Query: 121 AEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGT 180
           ++AL+YLHNGCEKR+LHRD+K SN+MLDS   AKLGDFGLAR I+++E THHST+ IAGT
Sbjct: 438 SQALEYLHNGCEKRILHRDIKASNVMLDSDFNAKLGDFGLARMIQQSEMTHHSTKEIAGT 497

Query: 181 YGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRT---SKNPSSGYEGHITNWVWEFQ 240
            GYMAPE FL  RAT ETDVYAFG+L+LEVV G+K +    K+  + Y   I NW+WE  
Sbjct: 498 PGYMAPETFLNGRATVETDVYAFGVLMLEVVSGKKPSYVLVKDNQNNYNNSIVNWLWELY 557

Query: 241 ERGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILP 300
             G I +A D  +G   ++EEM+  L++GLACCHPNP++RP+MK VLKVL GE  PP +P
Sbjct: 558 RNGTITDAADPGMGNLFDKEEMKSVLLLGLACCHPNPNQRPSMKTVLKVLTGETSPPDVP 617

Query: 301 HEMPSFVWP 307
            E P+FVWP
Sbjct: 618 TERPAFVWP 625

BLAST of MS022976 vs. TAIR 10
Match: AT3G53380.1 (Concanavalin A-like lectin protein kinase family protein )

HSP 1 Score: 294.7 bits (753), Expect = 8.8e-80
Identity = 148/309 (47.90%), Postives = 201/309 (65.05%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRD--VAVKRISENSRQGKQEFIAEVA 60
           F + ELK  T NF+    +G G FG VYRG L      VAVKR S +S+  K EF++E++
Sbjct: 364 FSYKELKAGTKNFNESRIIGHGAFGVVYRGILPETGDIVAVKRCSHSSQDKKNEFLSELS 423

Query: 61  TIGGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIIC 120
            IG L H+NLV+L GWC++K ++LL+Y+ MPNGSLDK +F S      T  W+ R+ I+ 
Sbjct: 424 IIGSLRHRNLVRLQGWCHEKGEILLVYDLMPNGSLDKALFESRF----TLPWDHRKKILL 483

Query: 121 GVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIA 180
           GVA AL YLH  CE +V+HRDVK SNIMLD    AKLGDFGLAR I   +    +    A
Sbjct: 484 GVASALAYLHRECENQVIHRDVKSSNIMLDESFNAKLGDFGLARQIEHDKSPEATV--AA 543

Query: 181 GTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKN-----PSSGYEGHITNWV 240
           GT GY+APE  LT RA+ +TDV+++G +VLEVV GR+   K+      + G   ++  WV
Sbjct: 544 GTMGYLAPEYLLTGRASEKTDVFSYGAVVLEVVSGRRPIEKDLNVQRHNVGVNPNLVEWV 603

Query: 241 WEFQERGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADP 300
           W   + G++  A D R+ G+ +E EM R L+VGLAC HP+P  RPTM++V+++L GEAD 
Sbjct: 604 WGLYKEGKVSAAADSRLEGKFDEGEMWRVLVVGLACSHPDPAFRPTMRSVVQMLIGEADV 663

Query: 301 PILPHEMPS 303
           P++P   P+
Sbjct: 664 PVVPKSRPT 666

BLAST of MS022976 vs. TAIR 10
Match: AT5G55830.1 (Concanavalin A-like lectin protein kinase family protein )

HSP 1 Score: 287.0 bits (733), Expect = 1.8e-77
Identity = 138/304 (45.39%), Postives = 196/304 (64.47%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRDV--AVKRISENSRQGKQEFIAEVA 60
           F + EL  AT  F     +GRG FG VYR   ++     AVKR   NS +GK EF+AE++
Sbjct: 353 FSYKELYTATKGFHSSRVIGRGAFGNVYRAMFVSSGTISAVKRSRHNSTEGKTEFLAELS 412

Query: 61  TIGGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIIC 120
            I  L HKNLV+L GWC +K +LLL+YE+MPNGSLDK+++      +   +W  R NI  
Sbjct: 413 IIACLRHKNLVQLQGWCNEKGELLLVYEFMPNGSLDKILYQESQTGAVALDWSHRLNIAI 472

Query: 121 GVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIA 180
           G+A AL YLH+ CE++V+HRD+K SNIMLD    A+LGDFGLAR +   +++  ST   A
Sbjct: 473 GLASALSYLHHECEQQVVHRDIKTSNIMLDINFNARLGDFGLAR-LTEHDKSPVSTL-TA 532

Query: 181 GTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQE 240
           GT GY+APE      AT +TD +++G+++LEV CGR+   K P S    ++ +WVW    
Sbjct: 533 GTMGYLAPEYLQYGTATEKTDAFSYGVVILEVACGRRPIDKEPESQKTVNLVDWVWRLHS 592

Query: 241 RGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPH 300
            G ++EA D R+ G+ +EE M++ L+VGL C HP+ +ERP+M+ VL++L  E +P  +P 
Sbjct: 593 EGRVLEAVDERLKGEFDEEMMKKLLLVGLKCAHPDSNERPSMRRVLQILNNEIEPSPVPK 652

Query: 301 EMPS 303
             P+
Sbjct: 653 MKPT 654

BLAST of MS022976 vs. TAIR 10
Match: AT5G01540.1 (lectin receptor kinase a4.1 )

HSP 1 Score: 276.9 bits (707), Expect = 1.9e-74
Identity = 140/298 (46.98%), Postives = 188/298 (63.09%), Query Frame = 0

Query: 3   FPELKKATGNFDPKNRLGRGGFGTVYRGNLMNRD-VAVKRISENSRQGKQEFIAEVATIG 62
           + +L  AT  F     +G GGFGTV++G L N D +AVK+I  +SRQG +EF+AE+ ++G
Sbjct: 357 YRDLYVATDGFKKTGIIGTGGFGTVFKGKLPNSDPIAVKKIIPSSRQGVREFVAEIESLG 416

Query: 63  GLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIICGVA 122
            L HKNLV L GWC  K DLLLIY+Y+PNGSLD L+++   +S    +W  R  I  G+A
Sbjct: 417 KLRHKNLVNLQGWCKHKNDLLLIYDYIPNGSLDSLLYTVPRRSGAVLSWNARFQIAKGIA 476

Query: 123 EALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIAGTY 182
             L YLH   EK V+HRDVKPSN+++DSK+  +LGDFGLAR   R   T   T  + GT 
Sbjct: 477 SGLLYLHEEWEKIVIHRDVKPSNVLIDSKMNPRLGDFGLARLYER--GTLSETTALVGTI 536

Query: 183 GYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQERGE 242
           GYMAPE+      ++ +DV+AFG+L+LE+VCGRK     P+      + +WV E    GE
Sbjct: 537 GYMAPELSRNGNPSSASDVFAFGVLLLEIVCGRK-----PTDSGTFFLVDWVMELHANGE 596

Query: 243 IVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPPILPHE 300
           I+ A D R+G  ++  E   AL VGL CCH  P  RP+M+ VL+ L GE + P +  E
Sbjct: 597 ILSAIDPRLGSGYDGGEARLALAVGLLCCHQKPASRPSMRIVLRYLNGEENVPEIDDE 647

BLAST of MS022976 vs. TAIR 10
Match: AT5G01560.1 (lectin receptor kinase a4.3 )

HSP 1 Score: 276.6 bits (706), Expect = 2.5e-74
Identity = 139/296 (46.96%), Postives = 187/296 (63.18%), Query Frame = 0

Query: 1   FEFPELKKATGNFDPKNRLGRGGFGTVYRGNLMNR--DVAVKRISENSRQGKQEFIAEVA 60
           F + +L KAT  F     +G GGFG VYRGN+ +    +AVK+I+ NS QG +EF+AE+ 
Sbjct: 351 FRYRDLYKATEGFKENRVVGTGGFGIVYRGNIRSSSDQIAVKKITPNSMQGVREFVAEIE 410

Query: 61  TIGGLHHKNLVKLMGWCYKKRDLLLIYEYMPNGSLDKLIFSSHDKSSPTPNWEIRRNIIC 120
           ++G L HKNLV L GWC  + DLLLIY+Y+PNGSLD L++S   +S    +W  R  I  
Sbjct: 411 SLGRLRHKNLVNLQGWCKHRNDLLLIYDYIPNGSLDSLLYSKPRRSGAVLSWNARFQIAK 470

Query: 121 GVAEALDYLHNGCEKRVLHRDVKPSNIMLDSKLEAKLGDFGLARAIRRTEQTHHSTRGIA 180
           G+A  L YLH   E+ V+HRDVKPSN+++DS +  +LGDFGLAR   R  Q+   T  + 
Sbjct: 471 GIASGLLYLHEEWEQIVIHRDVKPSNVLIDSDMNPRLGDFGLARLYERGSQS--CTTVVV 530

Query: 181 GTYGYMAPEIFLTRRATAETDVYAFGILVLEVVCGRKRTSKNPSSGYEGHITNWVWEFQE 240
           GT GYMAPE+     +++ +DV+AFG+L+LE+V GRK     P+      I +WV E Q 
Sbjct: 531 GTIGYMAPELARNGNSSSASDVFAFGVLLLEIVSGRK-----PTDSGTFFIADWVMELQA 590

Query: 241 RGEIVEAGDGRIGGQHEEEEMERALIVGLACCHPNPHERPTMKNVLKVLKGEADPP 295
            GEI+ A D R+G  ++E E   AL VGL CCH  P  RP M+ VL+ L  + D P
Sbjct: 591 SGEILSAIDPRLGSGYDEGEARLALAVGLLCCHHKPESRPLMRMVLRYLNRDEDVP 639

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151362.11.1e-18099.03probable L-type lectin-domain containing receptor kinase S.5 [Momordica charanti... [more]
XP_022151409.18.4e-16592.23probable L-type lectin-domain containing receptor kinase S.5 [Momordica charanti... [more]
XP_022158345.12.9e-14180.19LOW QUALITY PROTEIN: uncharacterized protein LOC111024852 [Momordica charantia][more]
XP_023527734.11.0e-13876.51probable L-type lectin-domain containing receptor kinase S.5 [Cucurbita pepo sub... [more]
XP_022935657.12.3e-13876.51probable L-type lectin-domain containing receptor kinase S.5 [Cucurbita moschata... [more]
Match NameE-valueIdentityDescription
Q9FG332.0e-11360.84Probable L-type lectin-domain containing receptor kinase S.5 OS=Arabidopsis thal... [more]
Q9LFH91.2e-7847.90L-type lectin-domain containing receptor kinase VIII.1 OS=Arabidopsis thaliana O... [more]
Q9FHG42.6e-7645.39Probable L-type lectin-domain containing receptor kinase S.7 OS=Arabidopsis thal... [more]
Q9M0212.7e-7346.98L-type lectin-domain containing receptor kinase VI.2 OS=Arabidopsis thaliana OX=... [more]
Q66GN23.5e-7346.96Lectin-domain containing receptor kinase VI.4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1DDA15.3e-18199.03probable L-type lectin-domain containing receptor kinase S.5 OS=Momordica charan... [more]
A0A6J1DD004.1e-16592.23probable L-type lectin-domain containing receptor kinase S.5 OS=Momordica charan... [more]
A0A6J1DVK51.4e-14180.19LOW QUALITY PROTEIN: uncharacterized protein LOC111024852 OS=Momordica charantia... [more]
A0A6J1F6711.1e-13876.51probable L-type lectin-domain containing receptor kinase S.5 OS=Cucurbita moscha... [more]
A0A6J1J0L65.5e-13876.43probable L-type lectin-domain containing receptor kinase S.5 OS=Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT5G06740.11.4e-11460.84Concanavalin A-like lectin protein kinase family protein [more]
AT3G53380.18.8e-8047.90Concanavalin A-like lectin protein kinase family protein [more]
AT5G55830.11.8e-7745.39Concanavalin A-like lectin protein kinase family protein [more]
AT5G01540.11.9e-7446.98lectin receptor kinase a4.1 [more]
AT5G01560.12.5e-7446.96lectin receptor kinase a4.3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 13..295
e-value: 6.1E-38
score: 142.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 16..285
e-value: 3.7E-51
score: 174.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 13..296
score: 39.014801
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 88..293
e-value: 1.3E-61
score: 209.5
NoneNo IPR availablePIRSFPIRSF000654ILKcoord: 4..290
e-value: 1.1E-31
score: 107.9
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 1..87
e-value: 2.7E-32
score: 112.9
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 11..213
e-value: 1.8E-15
score: 54.1
NoneNo IPR availablePANTHERPTHR27007:SF252L-TYPE LECTIN-DOMAIN CONTAINING RECEPTOR KINASE S.5-RELATEDcoord: 1..306
NoneNo IPR availablePANTHERPTHR27007FAMILY NOT NAMEDcoord: 1..306
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 19..289
e-value: 2.4427E-93
score: 276.076
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 135..147
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 19..40
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022976.1MS022976.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity