MS022518 (gene) Bitter gourd (TR) v1

BLAST of MS022518 vs. NCBI nr
Match: XP_022146713.1 (uncharacterized protein LOC111015850 [Momordica charantia])

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 789/809 (97.53%), Postives = 790/809 (97.65%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNS 60
           MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNP    
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNP---- 60

Query: 61  NSNSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPP 120
           NSNSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPP
Sbjct: 61  NSNSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPP 120

Query: 121 PPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGD 180
           PPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGD
Sbjct: 121 PPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGD 180

Query: 181 FEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAK 240
           FEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAK
Sbjct: 181 FEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAK 240

Query: 241 PPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMER 300
           PPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMER
Sbjct: 241 PPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMER 300

Query: 301 DRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMAVEDEQQQRPQFVITPRENQGFS 360
           DRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGM VEDEQQQRPQFVITPRENQGFS
Sbjct: 301 DRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFS 360

Query: 361 TIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVG--AS 420
           TIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVG  AS
Sbjct: 361 TIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASAS 420

Query: 421 ASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLVLNSQ 480
           ASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNL+LNSQ
Sbjct: 421 ASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLILNSQ 480

Query: 481 DSIHGWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDE 540
           DSIHGWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDE
Sbjct: 481 DSIHGWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDE 540

Query: 541 IRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFS 600
           IRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFS
Sbjct: 541 IRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFS 600

Query: 601 EGHTQPKSSSDNEEEEEEEEEDENSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRA 660
           EGHTQPKSSSDNEEEE      E SDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRA
Sbjct: 601 EGHTQPKSSSDNEEEE------ETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRA 660

Query: 661 ASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCC 720
           ASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCC
Sbjct: 661 ASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCC 720

Query: 721 MDMQLMLQQSIKQEVVGGKIAGGRGRKSKSRKPKCC-ICHEMQIDSLLYRCGHMCSCMKC 780
           MDMQLMLQQSIKQEVVG    GGRGRKSKSRKPKCC ICHEMQIDSLLYRCGHMCSCMKC
Sbjct: 721 MDMQLMLQQSIKQEVVG----GGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKC 780

Query: 781 AKELQWRGGKCPVCGAPIDDVVRASFTHS 807
           AKELQWRGGKCPVCGAPIDDVVRASFTHS
Sbjct: 781 AKELQWRGGKCPVCGAPIDDVVRASFTHS 795

BLAST of MS022518 vs. NCBI nr
Match: XP_038877490.1 (putative uncharacterized protein DDB_G0271606 [Benincasa hispida])

HSP 1 Score: 974.5 bits (2518), Expect = 5.4e-280
Identity = 578/854 (67.68%), Postives = 658/854 (77.05%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASSQVE SSSSSPFGCVLRDHNRR        +VA+FR+NLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVAATHVARFRDNLKTLVMDRLNDCITITPNK 60

Query: 61  NPNSNSNSNS-------NFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQR 120
           N N N N N        NFR+ +TN NT   + PRR++  +R+          T+I+  +
Sbjct: 61  NKNKNQNQNQNPNPVLPNFRVSKTNHNTTTNAAPRRANDSQRQ----------TSINDPQ 120

Query: 121 NNHHISPQTAPPPPPSPSK-ETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNAN-----A 180
                   + P P    +K +TSKLGASSLVQIWEKRLNVSS++VGLN N NA+     +
Sbjct: 121 TQTQTQTTSTPTPETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANASNTAVCS 180

Query: 181 KEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSS---SSTQSQSSDAG 240
            + E E EQEQACSVE GDFEDERYDAGPGSEDGFADWHS+RTSSS   SSTQSQSSDAG
Sbjct: 181 AKQETETEQEQACSVEVGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAG 240

Query: 241 ERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVE-HKCLS 300
           ER  ERVRV DIIRRLTLTAAKPPH S VED++ HPNESSS    +L LR+QVE  +CLS
Sbjct: 241 ER--ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNESSSSSHPTLILRDQVEPARCLS 300

Query: 301 HVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGM 360
           H+LCSPRIRGRQAFADLL+Q+ERDRQ+ELD L++RR VSKF QRGRIQSLLRLKILKRGM
Sbjct: 301 HILCSPRIRGRQAFADLLLQIERDRQRELDILVERRAVSKFSQRGRIQSLLRLKILKRGM 360

Query: 361 AVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEK-T 420
           A+EDE Q+RPQFVITPREN   STIMHLRE+FS VGENG RSPIGEMLNNN+ + ++  T
Sbjct: 361 ALEDE-QKRPQFVITPRENHRSSTIMHLREKFSGVGENGARSPIGEMLNNNDEDKNQSHT 420

Query: 421 QSDTDTHATCNN----DNDNHQVGASASAS------------ACVSDSDSAIHSSSMNTV 480
            + T  HAT  N    DNDN QV    S S              + + +          +
Sbjct: 421 NAHTPPHATNTNEKEKDNDNEQVVGIHSNSINNDQILEGFKEEQIEEREREQEQEQKQEI 480

Query: 481 DDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLVLNSQDSIHGWE---ESEAAEENYGAE 540
           +    P+  +EQ E+   DPP+SE TWQD PNL L+SQDSI+GWE   +SEAAEE+YGA 
Sbjct: 481 EQEQEPKQEQEQGEEV--DPPSSEGTWQDRPNLNLDSQDSINGWEAEDQSEAAEESYGA- 540

Query: 541 SDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFR 600
            DY+GTSY+WF+DISRPRSYWEDRRQSWYQQMLDS+SANDEIRQLI+RKTVS FL+SDFR
Sbjct: 541 -DYVGTSYDWFSDISRPRSYWEDRRQSWYQQMLDSSSANDEIRQLIQRKTVSTFLSSDFR 600

Query: 601 ERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEEEEE 660
           ERMDKLMV+RLE++TQ++EEY+E NE++D  +  EELWCFSEG TQ KSS + EEEE+E 
Sbjct: 601 ERMDKLMVTRLERQTQEEEEYDEVNEEDDD-DRAEELWCFSEGRTQSKSSDNGEEEEDER 660

Query: 661 -----EEDENSDYLDQSASPLQLASPSILSSWSYQ-DNEMGEDSNRAASMSSPQPFPPQF 720
                +  E SDYLDQS SPLQLASPSILSSWSYQ DNEMGEDSNR AS SSPQPF PQF
Sbjct: 661 SLISAQYHEASDYLDQSTSPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQF 720

Query: 721 SSNNQQ-SSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIK 780
           SSNNQQ SSLVS +HH SIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQ SIK
Sbjct: 721 SSNNQQGSSLVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIK 780

Query: 781 QEVVGGKIAGGRGRKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPV 805
           +  VGG   G R +K KSRKPKCCIC+ M+IDSLLYRCGHMCSCMKCAKELQWRGGKCPV
Sbjct: 781 RHEVGG---GRRSKKEKSRKPKCCICYNMEIDSLLYRCGHMCSCMKCAKELQWRGGKCPV 833

BLAST of MS022518 vs. NCBI nr
Match: XP_022977222.1 (uncharacterized protein LOC111477598 [Cucurbita maxima])

HSP 1 Score: 968.4 bits (2502), Expect = 3.9e-278
Identity = 585/852 (68.66%), Postives = 655/852 (76.88%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NPNSNSNS-NSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
           N N N N    NFR PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 61  NQNHNPNPVIPNFRGPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 120

Query: 121 SPQ--TAPPPPPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAK------EP 180
           +PQ  T P P     K  S   ASSLVQIWEKRLNVSS++VGLN N NANA       + 
Sbjct: 121 NPQIRTTPTPETGTDKNHSSKLASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVKQ 180

Query: 181 EPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSS---SSTQSQSSDAGERE 240
           E EQEQEQACS+EAGDF DERYDAG GSEDGFADWHS+RTSSS   SSTQSQ SDA  RE
Sbjct: 181 ETEQEQEQACSLEAGDFSDERYDAGLGSEDGFADWHSSRTSSSSPPSSTQSQISDA--RE 240

Query: 241 SERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLC 300
            ERVRV DIIRRLTLTAAKPPH S VEDND H +ESSS+PT  L LR QVE KCLSH+L 
Sbjct: 241 RERVRVVDIIRRLTLTAAKPPHSSWVEDND-HSSESSSNPT--LILRYQVEPKCLSHILY 300

Query: 301 SPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMAVED 360
           SPRIRGRQAFADLL+Q+ERDRQ+EL+ L++RR VSKFPQRGRIQSLLRLKIL+RGMA+ED
Sbjct: 301 SPRIRGRQAFADLLLQIERDRQRELEALVERRAVSKFPQRGRIQSLLRLKILQRGMALED 360

Query: 361 EQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTD 420
           E Q+RP+FVITPR N    TI HLRERFS  GENG RSPIGEML   +NNDD+K Q DTD
Sbjct: 361 E-QKRPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEML---DNNDDDKNQLDTD 420

Query: 421 --THATCNNDNDNHQVGASASASACVSDSDSAIHSSSMNTVDDHPIPED----------- 480
             THAT  NDNDN              D+D+   S++   V  +PIPED           
Sbjct: 421 AHTHATNANDNDN--------------DNDNDKDSNNQQVVGINPIPEDFNEEEIEEQEP 480

Query: 481 VKEQTEDQGHDPPTSEPTWQDTPNLVLNSQDSIHGWE---ESEAAEENYGAESDYMGTSY 540
           V+E   +   DPP+SE  WQD PNL L+SQDSI+GWE    SEAAEE+Y  + +Y+GTSY
Sbjct: 481 VQEPVPEPEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESY--DENYLGTSY 540

Query: 541 NWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMV 600
           +WF DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSNFL+S+FRERMDKLMV
Sbjct: 541 DWFADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMV 600

Query: 601 SRLEQRTQQQEEYNE--DNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEEEEEEEDEN- 660
           SRLE++TQQ+EEY++  + E+ +  EEEEELWCFSEGHTQP+ SSDNEEEEEEE+ DE  
Sbjct: 601 SRLERQTQQEEEYDDGAEEEEEEEEEEEEELWCFSEGHTQPE-SSDNEEEEEEEDRDERS 660

Query: 661 ----------SDYLDQSASPLQLASPSILSSWSYQ-DNEMGEDSNRAASMSSPQPFPPQF 720
                     SD LDQSASPLQ  SPSILSSWSYQ DNEMGEDSNR AS SSPQPF PQF
Sbjct: 661 LISSAPYQEASDDLDQSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQF 720

Query: 721 SSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIKQ 780
           SSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQ SIK+
Sbjct: 721 SSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKR 780

Query: 781 EVVGGKIAGGRGRKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 804
             VGG   G + +K KSRK KCCIC++MQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC
Sbjct: 781 HEVGG--GGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 811

BLAST of MS022518 vs. NCBI nr
Match: XP_022925581.1 (trichohyalin-like [Cucurbita moschata] >KAG7034825.1 Protein neuralized, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 965.3 bits (2494), Expect = 3.3e-277
Identity = 585/856 (68.34%), Postives = 653/856 (76.29%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NPNSNSNS-NSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
           N N N N    NFR+PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 61  NQNHNPNPVIPNFRVPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 120

Query: 121 SPQTAPPPPP---SPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA-------- 180
           +PQT   P P   +    +SKLGASSLVQIWEKRLNVSS++VGLN N NANA        
Sbjct: 121 NPQTRTTPTPQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVK 180

Query: 181 ------KEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSS---SSTQS 240
                 +E E EQEQEQACS+EAGDF DERYDAG GSED FADWHS+RTSSS   SSTQS
Sbjct: 181 QETEQEQEQEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQS 240

Query: 241 QSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVE 300
           Q SDA  RE ERVRV DIIRRLTLTAAKPPH S VEDND H NESSS+PT  L LR QVE
Sbjct: 241 QISDA--RERERVRVVDIIRRLTLTAAKPPHSSWVEDND-HSNESSSNPT--LILRYQVE 300

Query: 301 HKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKI 360
            KCLSH+L SPRIRGRQAFADLL+Q+ERDRQ+EL+TL++RR VSKFPQRGRIQSLLRLKI
Sbjct: 301 PKCLSHILYSPRIRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKI 360

Query: 361 LKRGMAVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNND 420
           L+RGMA+EDE Q+RP+FVITPR N    TI HLRERFS  GENG RSPIGEML   +NND
Sbjct: 361 LQRGMALEDE-QKRPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEML---DNND 420

Query: 421 DEKTQSDTD--THATCNNDNDNHQVGASASASACVSDSDSAIHSSSMNTVDDHPIPEDVK 480
           D+K Q DTD  THAT   DNDN      ++    V  +    H +     +    P    
Sbjct: 421 DDKNQLDTDPHTHATNTKDNDNDN-DKDSNNQQVVGINPIPEHFNEEEIEEKEEEPAQEP 480

Query: 481 EQTEDQGHDPPTSEPTWQDTPNLVLNSQDSIHGWE---ESEAAEENYGAESDYMGTSYNW 540
           E  ++Q  DPP+SE  WQD PNL L+SQDSI+GWE    SEAAEE+Y  + +Y+GTSY+W
Sbjct: 481 EPEQEQEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESY--DENYLGTSYDW 540

Query: 541 FTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMVSR 600
           F DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSNFL+S+FRERMDKLMVSR
Sbjct: 541 FADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMVSR 600

Query: 601 LEQRTQQQEEYNEDNEDNDGAEE------EEELWCFSEGHTQPKSSSDNEEEEEEEEED- 660
           LE++TQQ+EEY++  E+ D  EE      EEELWCFSEGHTQPK SSDNEEEEEEEEED 
Sbjct: 601 LERQTQQEEEYDDGAEEEDEEEEEEEEEGEEELWCFSEGHTQPK-SSDNEEEEEEEEEDR 660

Query: 661 ------------ENSDYLDQSASPLQLASPSILSSWSYQ-DNEMGEDSNRAASMSSPQPF 720
                       E SD LD SASPLQ  SPSILSSWSYQ DNEMGEDSNR AS SSPQPF
Sbjct: 661 DERSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPF 720

Query: 721 PPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQ 780
            PQFSSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQ 
Sbjct: 721 QPQFSSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQH 780

Query: 781 SIKQEVVGGKIAGGRGRKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGK 804
           SIK+  VGG   G + +K KSRK KCCIC++MQIDSLLYRCGHMCSCMKCAKELQWRGGK
Sbjct: 781 SIKRHEVGG--GGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRGGK 828

BLAST of MS022518 vs. NCBI nr
Match: KAG6581531.1 (Protein neuralized, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 965.3 bits (2494), Expect = 3.3e-277
Identity = 585/856 (68.34%), Postives = 653/856 (76.29%), Query Frame = 0

Query: 1    MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
            MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 515  MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 574

Query: 61   NPNSNSNS-NSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
            N N N N    NFR+PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 575  NQNHNPNPVIPNFRVPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 634

Query: 121  SPQTAPPPPP---SPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA-------- 180
            +PQT   P P   +    +SKLGASSLVQIWEKRLNVSS++VGLN N NANA        
Sbjct: 635  NPQTRTTPTPQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVK 694

Query: 181  ------KEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSS---SSTQS 240
                  +E E EQEQEQACS+EAGDF DERYDAG GSED FADWHS+RTSSS   SSTQS
Sbjct: 695  QETEQEQEQEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQS 754

Query: 241  QSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVE 300
            Q SDA  RE ERVRV DIIRRLTLTAAKPPH S VEDND H NESSS+PT  L LR QVE
Sbjct: 755  QISDA--RERERVRVVDIIRRLTLTAAKPPHSSWVEDND-HSNESSSNPT--LILRYQVE 814

Query: 301  HKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKI 360
             KCLSH+L SPRIRGRQAFADLL+Q+ERDRQ+EL+TL++RR VSKFPQRGRIQSLLRLKI
Sbjct: 815  PKCLSHILYSPRIRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKI 874

Query: 361  LKRGMAVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNND 420
            L+RGMA+EDE Q+RP+FVITPR N    TI HLRERFS  GENG RSPIGEML   +NND
Sbjct: 875  LQRGMALEDE-QKRPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEML---DNND 934

Query: 421  DEKTQSDTD--THATCNNDNDNHQVGASASASACVSDSDSAIHSSSMNTVDDHPIPEDVK 480
            D+K Q DTD  THAT   DNDN      ++    V  +    H +     +    P    
Sbjct: 935  DDKNQLDTDPHTHATNTKDNDNDN-DKDSNNQQVVGINPIPEHFNEEEIEEKEEEPAQEP 994

Query: 481  EQTEDQGHDPPTSEPTWQDTPNLVLNSQDSIHGWE---ESEAAEENYGAESDYMGTSYNW 540
            E  ++Q  DPP+SE  WQD PNL L+SQDSI+GWE    SEAAEE+Y  + +Y+GTSY+W
Sbjct: 995  EPEQEQEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESY--DENYLGTSYDW 1054

Query: 541  FTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMVSR 600
            F DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSNFL+S+FRERMDKLMVSR
Sbjct: 1055 FADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMVSR 1114

Query: 601  LEQRTQQQEEYNEDNEDNDGAEE------EEELWCFSEGHTQPKSSSDNEEEEEEEEED- 660
            LE++TQQ+EEY++  E+ D  EE      EEELWCFSEGHTQPK SSDNEEEEEEEEED 
Sbjct: 1115 LERQTQQEEEYDDGAEEEDEEEEEEEEEGEEELWCFSEGHTQPK-SSDNEEEEEEEEEDR 1174

Query: 661  ------------ENSDYLDQSASPLQLASPSILSSWSYQ-DNEMGEDSNRAASMSSPQPF 720
                        E SD LD SASPLQ  SPSILSSWSYQ DNEMGEDSNR AS SSPQPF
Sbjct: 1175 DERSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPF 1234

Query: 721  PPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQ 780
             PQFSSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQ 
Sbjct: 1235 QPQFSSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQH 1294

Query: 781  SIKQEVVGGKIAGGRGRKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGK 804
            SIK+  VGG   G + +K KSRK KCCIC++MQIDSLLYRCGHMCSCMKCAKELQWRGGK
Sbjct: 1295 SIKRHEVGG--GGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRGGK 1342

BLAST of MS022518 vs. ExPASy Swiss-Prot
Match: P29503 (Protein neuralized OS=Drosophila melanogaster OX=7227 GN=neur PE=1 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 3.8e-10
Identity = 33/64 (51.56%), Postives = 41/64 (64.06%), Query Frame = 0

Query: 744 RKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWR---GGKCPVCGAPIDDVVR 803
           ++S     +C IC+E  IDS+LY CGHMC C  CA E QWR   GG+CP+C A I DV+R
Sbjct: 692 QQSTDSSAECTICYENPIDSVLYMCGHMCMCYDCAIE-QWRGVGGGQCPLCRAVIRDVIR 751

Query: 804 ASFT 805
              T
Sbjct: 752 TYTT 754

BLAST of MS022518 vs. ExPASy Swiss-Prot
Match: Q24746 (Protein neuralized OS=Drosophila virilis OX=7244 GN=neur PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.8e-10
Identity = 33/64 (51.56%), Postives = 41/64 (64.06%), Query Frame = 0

Query: 744 RKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWR---GGKCPVCGAPIDDVVR 803
           ++S     +C IC+E  IDS+LY CGHMC C  CA E QWR   GG+CP+C A I DV+R
Sbjct: 685 QQSTDSSAECTICYENPIDSVLYMCGHMCMCYDCAIE-QWRGVGGGQCPLCRAVIRDVIR 744

Query: 804 ASFT 805
              T
Sbjct: 745 TYTT 747

BLAST of MS022518 vs. ExPASy TrEMBL
Match: A0A6J1CZA0 (uncharacterized protein LOC111015850 OS=Momordica charantia OX=3673 GN=LOC111015850 PE=4 SV=1)

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 789/809 (97.53%), Postives = 790/809 (97.65%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNS 60
           MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNP    
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNP---- 60

Query: 61  NSNSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPP 120
           NSNSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPP
Sbjct: 61  NSNSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPP 120

Query: 121 PPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGD 180
           PPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGD
Sbjct: 121 PPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGD 180

Query: 181 FEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAK 240
           FEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAK
Sbjct: 181 FEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAK 240

Query: 241 PPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMER 300
           PPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMER
Sbjct: 241 PPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMER 300

Query: 301 DRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMAVEDEQQQRPQFVITPRENQGFS 360
           DRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGM VEDEQQQRPQFVITPRENQGFS
Sbjct: 301 DRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFS 360

Query: 361 TIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVG--AS 420
           TIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVG  AS
Sbjct: 361 TIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASAS 420

Query: 421 ASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLVLNSQ 480
           ASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNL+LNSQ
Sbjct: 421 ASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLILNSQ 480

Query: 481 DSIHGWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDE 540
           DSIHGWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDE
Sbjct: 481 DSIHGWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDE 540

Query: 541 IRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFS 600
           IRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFS
Sbjct: 541 IRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFS 600

Query: 601 EGHTQPKSSSDNEEEEEEEEEDENSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRA 660
           EGHTQPKSSSDNEEEE      E SDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRA
Sbjct: 601 EGHTQPKSSSDNEEEE------ETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRA 660

Query: 661 ASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCC 720
           ASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCC
Sbjct: 661 ASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCC 720

Query: 721 MDMQLMLQQSIKQEVVGGKIAGGRGRKSKSRKPKCC-ICHEMQIDSLLYRCGHMCSCMKC 780
           MDMQLMLQQSIKQEVVG    GGRGRKSKSRKPKCC ICHEMQIDSLLYRCGHMCSCMKC
Sbjct: 721 MDMQLMLQQSIKQEVVG----GGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKC 780

Query: 781 AKELQWRGGKCPVCGAPIDDVVRASFTHS 807
           AKELQWRGGKCPVCGAPIDDVVRASFTHS
Sbjct: 781 AKELQWRGGKCPVCGAPIDDVVRASFTHS 795

BLAST of MS022518 vs. ExPASy TrEMBL
Match: A0A6J1IHV7 (uncharacterized protein LOC111477598 OS=Cucurbita maxima OX=3661 GN=LOC111477598 PE=4 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.9e-278
Identity = 585/852 (68.66%), Postives = 655/852 (76.88%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NPNSNSNS-NSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
           N N N N    NFR PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 61  NQNHNPNPVIPNFRGPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 120

Query: 121 SPQ--TAPPPPPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAK------EP 180
           +PQ  T P P     K  S   ASSLVQIWEKRLNVSS++VGLN N NANA       + 
Sbjct: 121 NPQIRTTPTPETGTDKNHSSKLASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVKQ 180

Query: 181 EPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSS---SSTQSQSSDAGERE 240
           E EQEQEQACS+EAGDF DERYDAG GSEDGFADWHS+RTSSS   SSTQSQ SDA  RE
Sbjct: 181 ETEQEQEQACSLEAGDFSDERYDAGLGSEDGFADWHSSRTSSSSPPSSTQSQISDA--RE 240

Query: 241 SERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLC 300
            ERVRV DIIRRLTLTAAKPPH S VEDND H +ESSS+PT  L LR QVE KCLSH+L 
Sbjct: 241 RERVRVVDIIRRLTLTAAKPPHSSWVEDND-HSSESSSNPT--LILRYQVEPKCLSHILY 300

Query: 301 SPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMAVED 360
           SPRIRGRQAFADLL+Q+ERDRQ+EL+ L++RR VSKFPQRGRIQSLLRLKIL+RGMA+ED
Sbjct: 301 SPRIRGRQAFADLLLQIERDRQRELEALVERRAVSKFPQRGRIQSLLRLKILQRGMALED 360

Query: 361 EQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTD 420
           E Q+RP+FVITPR N    TI HLRERFS  GENG RSPIGEML   +NNDD+K Q DTD
Sbjct: 361 E-QKRPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEML---DNNDDDKNQLDTD 420

Query: 421 --THATCNNDNDNHQVGASASASACVSDSDSAIHSSSMNTVDDHPIPED----------- 480
             THAT  NDNDN              D+D+   S++   V  +PIPED           
Sbjct: 421 AHTHATNANDNDN--------------DNDNDKDSNNQQVVGINPIPEDFNEEEIEEQEP 480

Query: 481 VKEQTEDQGHDPPTSEPTWQDTPNLVLNSQDSIHGWE---ESEAAEENYGAESDYMGTSY 540
           V+E   +   DPP+SE  WQD PNL L+SQDSI+GWE    SEAAEE+Y  + +Y+GTSY
Sbjct: 481 VQEPVPEPEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESY--DENYLGTSY 540

Query: 541 NWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMV 600
           +WF DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSNFL+S+FRERMDKLMV
Sbjct: 541 DWFADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMV 600

Query: 601 SRLEQRTQQQEEYNE--DNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEEEEEEEDEN- 660
           SRLE++TQQ+EEY++  + E+ +  EEEEELWCFSEGHTQP+ SSDNEEEEEEE+ DE  
Sbjct: 601 SRLERQTQQEEEYDDGAEEEEEEEEEEEEELWCFSEGHTQPE-SSDNEEEEEEEDRDERS 660

Query: 661 ----------SDYLDQSASPLQLASPSILSSWSYQ-DNEMGEDSNRAASMSSPQPFPPQF 720
                     SD LDQSASPLQ  SPSILSSWSYQ DNEMGEDSNR AS SSPQPF PQF
Sbjct: 661 LISSAPYQEASDDLDQSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQF 720

Query: 721 SSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIKQ 780
           SSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQ SIK+
Sbjct: 721 SSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKR 780

Query: 781 EVVGGKIAGGRGRKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 804
             VGG   G + +K KSRK KCCIC++MQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC
Sbjct: 781 HEVGG--GGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 811

BLAST of MS022518 vs. ExPASy TrEMBL
Match: A0A6J1ECL3 (trichohyalin-like OS=Cucurbita moschata OX=3662 GN=LOC111432973 PE=4 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.6e-277
Identity = 585/856 (68.34%), Postives = 653/856 (76.29%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NPNSNSNS-NSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
           N N N N    NFR+PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 61  NQNHNPNPVIPNFRVPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 120

Query: 121 SPQTAPPPPP---SPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA-------- 180
           +PQT   P P   +    +SKLGASSLVQIWEKRLNVSS++VGLN N NANA        
Sbjct: 121 NPQTRTTPTPQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVK 180

Query: 181 ------KEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSS---SSTQS 240
                 +E E EQEQEQACS+EAGDF DERYDAG GSED FADWHS+RTSSS   SSTQS
Sbjct: 181 QETEQEQEQEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQS 240

Query: 241 QSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVE 300
           Q SDA  RE ERVRV DIIRRLTLTAAKPPH S VEDND H NESSS+PT  L LR QVE
Sbjct: 241 QISDA--RERERVRVVDIIRRLTLTAAKPPHSSWVEDND-HSNESSSNPT--LILRYQVE 300

Query: 301 HKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKI 360
            KCLSH+L SPRIRGRQAFADLL+Q+ERDRQ+EL+TL++RR VSKFPQRGRIQSLLRLKI
Sbjct: 301 PKCLSHILYSPRIRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKI 360

Query: 361 LKRGMAVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNND 420
           L+RGMA+EDE Q+RP+FVITPR N    TI HLRERFS  GENG RSPIGEML   +NND
Sbjct: 361 LQRGMALEDE-QKRPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEML---DNND 420

Query: 421 DEKTQSDTD--THATCNNDNDNHQVGASASASACVSDSDSAIHSSSMNTVDDHPIPEDVK 480
           D+K Q DTD  THAT   DNDN      ++    V  +    H +     +    P    
Sbjct: 421 DDKNQLDTDPHTHATNTKDNDNDN-DKDSNNQQVVGINPIPEHFNEEEIEEKEEEPAQEP 480

Query: 481 EQTEDQGHDPPTSEPTWQDTPNLVLNSQDSIHGWE---ESEAAEENYGAESDYMGTSYNW 540
           E  ++Q  DPP+SE  WQD PNL L+SQDSI+GWE    SEAAEE+Y  + +Y+GTSY+W
Sbjct: 481 EPEQEQEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESY--DENYLGTSYDW 540

Query: 541 FTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMVSR 600
           F DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSNFL+S+FRERMDKLMVSR
Sbjct: 541 FADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMVSR 600

Query: 601 LEQRTQQQEEYNEDNEDNDGAEE------EEELWCFSEGHTQPKSSSDNEEEEEEEEED- 660
           LE++TQQ+EEY++  E+ D  EE      EEELWCFSEGHTQPK SSDNEEEEEEEEED 
Sbjct: 601 LERQTQQEEEYDDGAEEEDEEEEEEEEEGEEELWCFSEGHTQPK-SSDNEEEEEEEEEDR 660

Query: 661 ------------ENSDYLDQSASPLQLASPSILSSWSYQ-DNEMGEDSNRAASMSSPQPF 720
                       E SD LD SASPLQ  SPSILSSWSYQ DNEMGEDSNR AS SSPQPF
Sbjct: 661 DERSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPF 720

Query: 721 PPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQ 780
            PQFSSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQ 
Sbjct: 721 QPQFSSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQH 780

Query: 781 SIKQEVVGGKIAGGRGRKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGK 804
           SIK+  VGG   G + +K KSRK KCCIC++MQIDSLLYRCGHMCSCMKCAKELQWRGGK
Sbjct: 781 SIKRHEVGG--GGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRGGK 828

BLAST of MS022518 vs. ExPASy TrEMBL
Match: A0A1S3AYB9 (uncharacterized protein LOC103484234 OS=Cucumis melo OX=3656 GN=LOC103484234 PE=4 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 1.3e-268
Identity = 570/845 (67.46%), Postives = 645/845 (76.33%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASSQVEFSSSSSPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNQ 60

Query: 61  NPNSNSNSNSNFRLPRTNENTANPSTPRRSSILERRPTK-PLHRTLSTNIDRQRNNHHIS 120
           N N      +NF + +TN +T   ST R     +   T  P  +T ST            
Sbjct: 61  NTNP---VLTNFPVTKTNHDT---STKRSDDSQQHASTNDPQTQTTST------------ 120

Query: 121 PQTAPP-PPPSPSK-ETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA------KEPE 180
              APP P  SP+K +TSKLGASSLVQIWEKRLNVSS+++GLN N NAN       +E E
Sbjct: 121 --PAPPIPETSPNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETE 180

Query: 181 PEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSS---SSTQSQSSDAGERES 240
            EQEQEQACSVEAGDFEDERYDAGPGS+DGFADWHS+RTSSS   SSTQSQSSDAGER  
Sbjct: 181 AEQEQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGER-- 240

Query: 241 ERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSS-HPTLSLSLREQVEHKCLSHVLC 300
           ERVRV DIIRRLTLTAAKPPH S VED++  PNESSS HPT  L  ++QVE KCLSH+LC
Sbjct: 241 ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPT--LIPKDQVEPKCLSHILC 300

Query: 301 SPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMAVED 360
           SPRIRGRQAFADLL+Q+ERDRQ+ELD L++RR VSKFPQRGRIQSLLRLKILKRGMA+ED
Sbjct: 301 SPRIRGRQAFADLLLQIERDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALED 360

Query: 361 EQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDT- 420
           E Q+RPQFVITPREN    TIMHLRERFS VGENG +SP GEML   N+NDD+KTQ DT 
Sbjct: 361 E-QKRPQFVITPRENHRSFTIMHLRERFSGVGENGAKSPRGEML---NDNDDDKTQPDTN 420

Query: 421 -DTHA--------TCNNDNDNHQVGASASASACVSDSDSAIHSSSMNTVDDHPIPEDVKE 480
            +TH           + DNDN QV    S +   +D+D  I       ++      D + 
Sbjct: 421 ANTHTPPHVINTNEKDKDNDNQQVVGIHSNN---TDNDQIIGGFKEEQIEKQEQKPDQEL 480

Query: 481 QTEDQGH-DPPTSEPTWQDTPNLVLNSQDSIHGWE---ESEAAEENYGAESDYMGTSYNW 540
           + E  G  DPP+ E  WQD   L  +S DS++GWE   +SEA EE+YGA  DY+GTSY+W
Sbjct: 481 EPEQGGEVDPPSLEGIWQDGSKLNSDSHDSMNGWEAEYQSEAGEESYGA--DYVGTSYDW 540

Query: 541 FTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMVSR 600
           F DISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLI+RKTVSNFL+SDFRERMDKLMV+R
Sbjct: 541 FADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTR 600

Query: 601 LEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEEEEE------EEDE 660
           LE++TQQ+EEYNE  ED+D    +EELWCFSEGHTQPK SSDNEEE+++E      +  E
Sbjct: 601 LERQTQQEEEYNEVKEDDD---VDEELWCFSEGHTQPK-SSDNEEEDDDERSLITAQYHE 660

Query: 661 NSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFPPQFSSNNQQ--SS 720
            SDYLDQSASPLQLASPSILSSWSY   +MGEDSNR  S+SSPQP  PQFSSNN Q   S
Sbjct: 661 ASDYLDQSASPLQLASPSILSSWSY---DMGEDSNRGTSISSPQPLLPQFSSNNNQHRPS 720

Query: 721 LVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIKQEVVGGKIA 780
           LVS SH+ SIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQ SIK+     ++ 
Sbjct: 721 LVSASHNPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKR---SHEVG 780

Query: 781 GGRGRKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCGAPIDDVV 805
           G + +K KSRKPKCCIC+ M+IDSLLYRCGHMCSCMKC KELQWRGGKCPVC API+DVV
Sbjct: 781 GRKSKKDKSRKPKCCICYNMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRAPIEDVV 802

BLAST of MS022518 vs. ExPASy TrEMBL
Match: A0A5A7U932 (Altered inheritance of mitochondria protein 44 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00080 PE=4 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 1.1e-267
Identity = 572/852 (67.14%), Postives = 645/852 (75.70%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASSQVEFSSSSSPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNQ 60

Query: 61  NPNSNSNSNSNFRLPRTNENTANPSTPRRSSILERRPTK-PLHRTLSTNIDRQRNNHHIS 120
           N N      +NF + +TN +T   ST R     +   T  P  +T ST            
Sbjct: 61  NTNP---VLTNFPVTKTNHDT---STKRSDDSQQHASTNDPQTQTTST------------ 120

Query: 121 PQTAPP-PPPSPSK-ETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA---------- 180
              APP P  SP+K +TSKLGASSLVQIWEKRLNVSSN +GLN N NANA          
Sbjct: 121 --PAPPIPETSPNKNQTSKLGASSLVQIWEKRLNVSSN-IGLNANANANANANANTAVCS 180

Query: 181 --KEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSS---SSTQSQSSD 240
             +E E EQEQEQACSVEAGDFEDERYDAGPGS+DGFADWHS+RTSSS   SSTQSQSSD
Sbjct: 181 TKQETEAEQEQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSD 240

Query: 241 AGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSS-HPTLSLSLREQVEHKC 300
           AGER  ERVRV DIIRRLTLTAAKPPH S VED++  PNESSS HPT  L  ++QVE KC
Sbjct: 241 AGER--ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPT--LIPKDQVEPKC 300

Query: 301 LSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKR 360
           LSH+LCSPRIRGRQAFADLL+Q+ERDRQ+ELD L++RR VSKFPQRGRIQSLLRLKILKR
Sbjct: 301 LSHILCSPRIRGRQAFADLLLQIERDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKR 360

Query: 361 GMAVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEK 420
           GMA+EDE Q+RPQFVITPREN    TIMHLRERFS VGENG +SP GEML   N+NDD+K
Sbjct: 361 GMALEDE-QKRPQFVITPRENHRSFTIMHLRERFSGVGENGAKSPRGEML---NDNDDDK 420

Query: 421 TQSDT--DTHA--------TCNNDNDNHQVGASASASACVSDSDSAIHSSSMNTVDDHPI 480
           TQ DT  +TH           + DNDN QV    S +   +D+D  I       ++    
Sbjct: 421 TQPDTNANTHTPPHVINTNEKDKDNDNQQVVGIHSNN---TDNDQIIGGFKEEQIEKQEQ 480

Query: 481 PEDVKEQTEDQGH-DPPTSEPTWQDTPNLVLNSQDSIHGWE---ESEAAEENYGAESDYM 540
             D + + E  G  DPP+ E  WQD   L  +S DS++GWE   +SEA EE+YGA  DY+
Sbjct: 481 KPDQELEPEQGGEVDPPSLEGIWQDGSKLNSDSHDSMNGWEAEYQSEAGEESYGA--DYV 540

Query: 541 GTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMD 600
           GTSY+WF DISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLI+RKTVSNFL+SDFRERMD
Sbjct: 541 GTSYDWFADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMD 600

Query: 601 KLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEEEEEEED- 660
           KLMV+RLE++TQQ+EEYNE  ED+D    +EELWCFSEGHTQPK SSDNEEE++++E   
Sbjct: 601 KLMVTRLERQTQQEEEYNEVKEDDD---VDEELWCFSEGHTQPK-SSDNEEEDDDDERSL 660

Query: 661 ------ENSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFPPQFSSN 720
                 E SDYLDQSASPLQLASPSILSSWSY   +MGEDSNR  S+SSPQP  PQFSSN
Sbjct: 661 ITAQYHEASDYLDQSASPLQLASPSILSSWSY---DMGEDSNRGTSISSPQPLLPQFSSN 720

Query: 721 NQQ--SSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIKQE 780
           N Q   SLVS SH+ SIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQ SIK+ 
Sbjct: 721 NNQHRPSLVSASHNPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKR- 780

Query: 781 VVGGKIAGGRGRKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCG 805
               ++ G + +K KSRKPKCCIC+ M+IDSLLYRCGHMCSCMKC KELQWRGGKCPVC 
Sbjct: 781 --SHEVGGRKSKKDKSRKPKCCICYNMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCR 808

BLAST of MS022518 vs. TAIR 10
Match: AT2G34920.1 (RING/U-box superfamily protein )

HSP 1 Score: 238.0 bits (606), Expect = 2.6e-62
Identity = 251/815 (30.80%), Postives = 372/815 (45.64%), Query Frame = 0

Query: 15  FGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNSNSNSNFRLPRTNEN 74
           FG VLRD N+R+ +   F+ NLK  V          T  P  +  S+ N    L      
Sbjct: 8   FGSVLRDRNQRHNDDVVFKKNLKAQVK---------TAPPAISDESSENRVDSLIGNKRK 67

Query: 75  TANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSPSKETSKLGAS 134
             N S P         P KP          R R  ++ S                  GAS
Sbjct: 68  KNNKSRP-------GSPEKP----------RTRKGNNFSDNLG--------------GAS 127

Query: 135 SLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDERYDAGPGSED 194
           SLVQIWE RLN S+      GN   +++  E   E     SV+      E +   P S D
Sbjct: 128 SLVQIWEARLNRSN-----GGNSAIHSQSIEISSE----ASVQ------EIHLLAP-SID 187

Query: 195 GFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHP 254
           G ++   +   S S  Q+   ++G   S    V+DIIRRL+              N+Q  
Sbjct: 188 GESE---SENESKSPDQTVEIESGTLNS----VSDIIRRLS--------------NEQKL 247

Query: 255 NESSSHPTLSLSL--REQVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDR 314
             S++   + + +     +E      V CSPRIRGRQA++DLL+ +ER+R +EL++LL R
Sbjct: 248 TASNNGGAVDMPIVKTPTLEKSSFQVVTCSPRIRGRQAYSDLLVHLERERHRELESLLGR 307

Query: 315 RPVSKFPQRGRIQSLLRLKILKRGMAVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRV 374
             VS+FPQRGR+QS+LRL+ LKRG+A++D  +   +  +     Q  STI+HLRE+    
Sbjct: 308 NAVSRFPQRGRLQSMLRLRSLKRGLAIQDRHRGTTKSDL--NRFQPSSTILHLREKLREK 367

Query: 375 GENG---------------------------LRSPIGEMLNNNNNNDDEKTQSDTDTHAT 434
             N                            L SP  E L+    N +E      +T   
Sbjct: 368 AANAAAEAGLKKGQQSTVETESMQSKETSGILHSPSTERLSPQKRNIEEAILRKNETETK 427

Query: 435 CNNDNDNHQVGASASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEP 494
            +      Q+  +  A     +SD+    +S+   +   +      + E+ G     +E 
Sbjct: 428 MS----YLQLKKAIVAEVLERESDNTSPLTSVTPQEPRIL------RNEEAGKLESGTEG 487

Query: 495 TWQDTPNLVLNSQDSIHGWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSW 554
           T Q+TP L         GWEE E  E+    +S Y   SY+WFT+ISRPR+YWED R+S 
Sbjct: 488 T-QETPFLETQEMSFQSGWEEQEEYEDE---QSYYGDMSYDWFTEISRPRTYWEDLRKSR 547

Query: 555 YQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDN 614
           Y +++++ S  D+I +L+ER+TVS FL S  RE++DKL++SR++                
Sbjct: 548 YLEVMNTKSDKDDICRLLERRTVSGFLQSGLREKIDKLIMSRVQ---------------- 607

Query: 615 DGAEEEEELWCFSEGHTQPKSSSDNEEEEEEEEEDENSDYLDQSASPLQLASPSILSSWS 674
                          H   +++ + E+ +  EE+DE+ D L QS+S  Q+ +PS   SWS
Sbjct: 608 -----------IHPAHRIEEATKEEEKYDIGEEKDEDRDDLSQSSS--QIFAPSPAGSWS 645

Query: 675 YQDNEMGEDSNRAASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLY 734
             D  +                          +S  + + H+++++              
Sbjct: 668 SLDTGV--------------------------TSTPTHNLHSTLQL-------------- 645

Query: 735 QEMSELRKSIKCCMDMQLMLQQSIKQEVVGGKIAGGRGRKSKSRKPKCCICHEMQIDSLL 794
            EMSELR S+K C+D+   LQ+S+  E                 K KCC+C+E Q+++LL
Sbjct: 728 -EMSELRDSVKTCLDVNASLQKSVHLE--------------NPFKRKCCVCNETQVETLL 645

Query: 795 YRCGHMCSCMKCAKELQWRGGKCPVCGAPIDDVVR 801
           YRCGHMC+C++CA ELQ+ GGKCP+C A I DVVR
Sbjct: 788 YRCGHMCTCLRCANELQYNGGKCPICHAKILDVVR 645

BLAST of MS022518 vs. TAIR 10
Match: AT1G30860.1 (RING/U-box superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 1.8e-60
Identity = 259/861 (30.08%), Postives = 382/861 (44.37%), Query Frame = 0

Query: 10  SSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNSNSNSNFRLP 69
           SS   FG +LRD   RN N   F+ NLK+ V + L   +         S+ N   ++   
Sbjct: 3   SSPQRFGLILRD---RNQNAGVFQKNLKSPVKEVLKSHV---------SDENLVDSWIET 62

Query: 70  RTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSPSKETS 129
             N+N                     +R L + ++ +RN++                   
Sbjct: 63  HNNKN---------------------NRVLESPLESKRNDN------------------- 122

Query: 130 KLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDERYDAG 189
            L ASSLVQIWE RLN S+        GN+      P   Q  A S    D      D+G
Sbjct: 123 -LVASSLVQIWEARLNRSN-------GGNS------PSSSQSTASSSSRSDSGVSVQDSG 182

Query: 190 ---PGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHPSL 249
                S DG ++         S +    SD+G  ES+  RVAD+IRRL+           
Sbjct: 183 LSESSSIDGDSEIADRTVEIESRSHGLVSDSG--ESKWGRVADLIRRLS----------- 242

Query: 250 VEDNDQHPNESSSHPTLSLSLREQVEHKCLSH---------VLCSPRIRGRQAFADLLMQ 309
            ED  +   ++ S     L +       C S          V  SP+IRGRQAF D LMQ
Sbjct: 243 NEDKKRTAGDNGSGGGGGLEIIRTPRPYCTSSSSEKSNFPVVSFSPKIRGRQAFTDFLMQ 302

Query: 310 MERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMAVEDEQQQRPQFVITPRENQ 369
           MERDR +ELD+L +R  VS+F QRGR+QS+LRL+ L R + ++D  +   +     R   
Sbjct: 303 MERDRHRELDSLFERNAVSRFTQRGRLQSMLRLRNLNRCLVIQDRNRSNAKTTGLNRIES 362

Query: 370 GFSTIMHLRERF--------------------------------------SRVGENG--L 429
           G S ++HLRE+F                                      +R+ + G  L
Sbjct: 363 G-SAVLHLREKFHANVVNSAAATDKRKNHQRSTEMNNKSVEETEGTTTTSTRLKDGGVTL 422

Query: 430 RSPIGEMLNNNNNNDDEKT--QSDTDTHATCNNDNDNHQVGASASASACVSDSDSAIHSS 489
            +   E L   N   +E T  + +     T ++  +  Q+  +       +D  S     
Sbjct: 423 EAFFAERLRPRNRKIEEATLCKEEESVSGTVDSQKNCLQLQETREVEETCNDGKSEKKEE 482

Query: 490 SMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLVLNSQDSIHGWEESEAA----- 549
              +  ++   E    Q +D+ +     + T ++T  LV  S +     ++ E +     
Sbjct: 483 ENTSPSEYLSRESRLRQNQDENNVGKYMQET-RETEGLVHESNEMDQCLDQQETSYLNRW 542

Query: 550 --EENYGAESDYMGTSYN--WFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERK 609
             +E Y  E  Y G  YN  W ++I+RPRSYWE+ R+S Y +++++ S  D+I +L+ER+
Sbjct: 543 GEQEEYADEQSYYG-EYNDDWLSEIARPRSYWEELRKSRYLEVMNTRSEKDDICRLLERR 602

Query: 610 TVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHTQPKS 669
           TV++FL S  RE++D LM+SR++  + +  +  E  +     EEEEE+            
Sbjct: 603 TVTDFLDSGLREKIDNLMMSRVQTHSDKHSKKWELQQ-----EEEEEV----------NF 662

Query: 670 SSDNEEEEEE----EEEDENSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMS 729
             D E +EE     EE+D+  D    S+S +  +SP+   SWS QD ++   S  A  + 
Sbjct: 663 EIDEEIKEEPLRGGEEQDDRDDLSQSSSSHISASSPA--GSWSSQDTDV--TSTPALVVQ 722

Query: 730 SPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQ 789
           +PQ                      S EMELI  +R  ++QL QEMS LR S+K C+D  
Sbjct: 723 NPQ----------------------SPEMELISGMRSQIQQLQQEMSVLRDSVKTCLDAN 726

Query: 790 LMLQQSIKQEVVGGKIAGGRGRKSKSRKPKCCICHEMQIDSLLYRCGHMCSCMKCAKELQ 804
             LQ    QE                 K KCC+C E Q++++LYRCGHMC C+KCA EL 
Sbjct: 783 ASLQHKAHQE--------------NPMKRKCCVCDETQVEAVLYRCGHMCMCLKCANELH 726

BLAST of MS022518 vs. TAIR 10
Match: AT4G20160.1 (LOCATED IN: chloroplast; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, 4 anthesis, petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: RING/U-box superfamily protein (TAIR:AT1G30860.1); Has 64917 Blast hits to 39974 proteins in 1949 species: Archae - 279; Bacteria - 7425; Metazoa - 26604; Fungi - 5788; Plants - 3193; Viruses - 275; Other Eukaryotes - 21353 (source: NCBI BLink). )

HSP 1 Score: 141.7 bits (356), Expect = 2.5e-33
Identity = 128/378 (33.86%), Postives = 197/378 (52.12%), Query Frame = 0

Query: 399  DTDTHATCNNDNDNHQVGASASASACVSDSDSAIHSSSMNTVDD---HPIPEDVK--EQT 458
            +++  +T   + +  Q  +    SA     +SA   +S    D+   H  P D K  ++ 
Sbjct: 827  NSEAKSTKEIERNKSQEVSQGEESASHGSRESAKEKNSSQQDDETSTHRNPNDKKGIKEP 886

Query: 459  EDQGHDPPTSEPTWQDTPNLVLNSQDSIHGWEESEAAEENYGAESDYMGTSYNWFTDISR 518
            ED+       E T +   N+   S + ++ W+ +E  EE      DY     +W  DISR
Sbjct: 887  EDEESKKVEREETGE---NVEEASVEFVNDWDGNEMEEEEEEEYGDYFNGDDDWIHDISR 946

Query: 519  PRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQ 578
            PRSYWED R+  Y ++L++ S   +I  LIER+TVSNFLTSD R+++D LM++R++    
Sbjct: 947  PRSYWEDLRKERYLEVLNTESEKKDICNLIERRTVSNFLTSDLRQKIDNLMITRVQSHIG 1006

Query: 579  QQEEYNEDNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEEEEEEE--DENSDYLDQSAS 638
                    N+  +G E EEE W   E     ++  DNE EEE E+   +  SD   QS+ 
Sbjct: 1007 VPL-----NQIEEGDEYEEE-W---EVECSARNEEDNETEEEPEKTNLEAPSDVCSQSS- 1066

Query: 639  PLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEM 698
                A  S + SW+++D ++ +D+    S+S P+P  P              ++ ++ +M
Sbjct: 1067 ----ARSSTMMSWNFRDQDIDKDNEPTTSLSLPEPLVP--------------TNQSTQDM 1126

Query: 699  ELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIKQEVV-GGKIAGGR-GRKSKSR 758
            + I DL+  MEQL +EM ELR ++K C+DMQL  Q+S+ Q++   G  A  R   K    
Sbjct: 1127 QTISDLKEQMEQLQREMLELRNTVKSCIDMQLHFQKSVTQDLSRSGSSAEQRVDPKKDPL 1173

Query: 759  KPKCCICHEMQIDSLLYR 768
            K KCC+C EM +DSLLYR
Sbjct: 1187 KRKCCVCSEMPVDSLLYR 1173

HSP 2 Score: 105.9 bits (263), Expect = 1.5e-22
Identity = 126/420 (30.00%), Postives = 182/420 (43.33%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVL---------RDHNR-------------RNANVAKFRNNLKT 60
           MASS+VE SS  + F CVL         RD+N              RN +VA F  NL  
Sbjct: 1   MASSEVEMSSCQT-FNCVLSRPHERCTQRDNNTNNNKKKNNNNNNVRNIHVAAFEKNLNV 60

Query: 61  LVMDRLNDCI---------SITPNPNPNSNSNSNSNFRLPRTNENTANPSTPRRSSILER 120
            V D L +C          SI   P  +SN +S S+ RL +T E  +    P  SS    
Sbjct: 61  FVRDHLENCSVSVDDVVDDSIKAVPECSSNKSSVSDHRLSKTEE--SRQDVPSSSS---- 120

Query: 121 RPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSPSKETSKLGASSLVQIWEKR------ 180
                   +L ++ D         P +  P  P      S+  ASSLVQIWE R      
Sbjct: 121 --------SLGSDSD---------PNSGQPENP------SRNAASSLVQIWEARTTQQPP 180

Query: 181 ------LNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFA 240
                 ++  ++ +G N   N+ + E   E +  Q       + E+E  +  P       
Sbjct: 181 SSNQSLIDSRTSSMGSNALENSESLESVKESQMIQPIEECNNEEEEEEIECAP------- 240

Query: 241 DWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNES 300
                             ++GE++ E VRV DIIR+L+  +    +     DN    N++
Sbjct: 241 ---------------PPLESGEKDREGVRVMDIIRKLSNDSETITN----NDNGSSGNDN 300

Query: 301 SSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSK 360
           S          +  E +    V CSPRIRGRQA ADLL+QM RDR+K+L  L +R  VSK
Sbjct: 301 SKEV-------QTTEARSFPQVACSPRIRGRQALADLLVQMTRDREKDLACLRERHCVSK 357

Query: 361 FPQRGRIQSLLRLKILKRGMAVEDEQQQRPQFV--ITPRENQGFSTIMHLRERFSRVGEN 376
           F  RGRIQS LR++  +R +A++   + +       + R ++G   +  LRE++    EN
Sbjct: 361 FTNRGRIQSTLRIRCYERCLAIQGRHRSKSTSAGSDSNRSSRGSGVMHLLREKYKANSEN 357

BLAST of MS022518 vs. TAIR 10
Match: AT5G04460.1 (RING/U-box superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 5.6e-33
Identity = 196/698 (28.08%), Postives = 302/698 (43.27%), Query Frame = 0

Query: 156 NGNANAKEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSS 215
           N N +++E  P          + GD E ER             W  +R +  SS   Q  
Sbjct: 230 NNNTSSREQSP----------DLGDGERERV------RHIARGWMDSRINDHSSNVRQRD 289

Query: 216 D------AGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLRE 275
           D       G+ E ERVR+     ++T         +  ED         +H       R 
Sbjct: 290 DNRRPEWLGDTERERVRIIREWMQMTSQQRGGARATPREDQRSTSEADRNHDAAPQVDRV 349

Query: 276 QVEHKCLSHVLCSP--------RIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQR 335
           +V    ++H    P        R+RGRQA  DLLM+ ER+RQ+EL  LL+ R VS F  R
Sbjct: 350 RV-GLAVNHEEGQPPHVRRDLRRVRGRQALLDLLMRAERERQRELQGLLEHRAVSDFAHR 409

Query: 336 GRIQSLLRLKILK--RGMAVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRS 395
            RIQSLLR + L+  R    E       + ++  RE Q   T+  LRE F     NG  +
Sbjct: 410 NRIQSLLRGRFLRNERPTVPERTPSMASRELLQLRERQ---TVSGLREGF----HNGREN 469

Query: 396 PIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQV-GASASASACVSDSDSAIHSSSMN 455
            + E   N +N D++ + + T+  A      ++ +V   S+++S   +DS     +S  N
Sbjct: 470 IVHE---NTSNTDNDNSNTSTNALAIAITAGNSQRVTDESSTSSRQGNDSPILPDNSESN 529

Query: 456 TVD-DHPIPEDVKEQTEDQGHDPPTSEPTWQDT--------PNLVLNSQDS--------I 515
             + D    ED  ++   Q + P    P  + T         N  +N  ++         
Sbjct: 530 LANADRDWEEDTNQRRIWQENVPVDERPNLEQTTLTQFDGYDNTDINRDETSVSDMHREA 589

Query: 516 HGWEESE-AAEENYGA--ESDYMGTSYNW---FTDISRPRSYWEDRRQSWYQQMLDSNSA 575
            G+ + E   +E +G   E+    +  NW    ++  R R   + RR + +    D N  
Sbjct: 590 SGFADDEYRTQEAHGVWHENSSRQSDGNWPGTRSEALRSRRVVQLRRLNRFHPPEDDNVY 649

Query: 576 NDEIRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELW 635
           + E+R+L+ R++VSN L S FRE +D+L+ S  E+R     +++                
Sbjct: 650 SMELRELLSRRSVSNLLHSGFRESLDQLIQSYAERRGHTHVDWD---------------- 709

Query: 636 CFSEGHTQPKSSSDNEEEEEEEEEDENSDYLDQSASPLQLASPSILSSWSYQDNEMGEDS 695
                + Q       E + + +    N + L+       L +P                 
Sbjct: 710 --LHANLQTAIPDSPERDTDHQVFVRNDNQLNGINGSQLLPTP----------------- 769

Query: 696 NRAASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSI 755
                  +P P P      +  S      H + IE E++ DLRG + +L Q MS++++ +
Sbjct: 770 ------PAPPPQPIWHQDLHHTSWSRHSMHRSEIEWEVMNDLRGDVARLQQGMSQMQRML 829

Query: 756 KCCMDMQLMLQQSIKQEVVG--GKIAGGRG----------RKSKSRKPKCCICHEMQIDS 802
           + CMDMQL LQ+S++QEV     + AG +G          R S   K  CC+C +  ID+
Sbjct: 830 EACMDMQLELQRSVRQEVSAALNRSAGDQGMSAETSEDGSRWSHVSKGTCCVCCDNHIDA 859

BLAST of MS022518 vs. TAIR 10
Match: AT5G04460.3 (RING/U-box superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 5.6e-33
Identity = 196/698 (28.08%), Postives = 302/698 (43.27%), Query Frame = 0

Query: 156 NGNANAKEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSS 215
           N N +++E  P          + GD E ER             W  +R +  SS   Q  
Sbjct: 230 NNNTSSREQSP----------DLGDGERERV------RHIARGWMDSRINDHSSNVRQRD 289

Query: 216 D------AGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLRE 275
           D       G+ E ERVR+     ++T         +  ED         +H       R 
Sbjct: 290 DNRRPEWLGDTERERVRIIREWMQMTSQQRGGARATPREDQRSTSEADRNHDAAPQVDRV 349

Query: 276 QVEHKCLSHVLCSP--------RIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQR 335
           +V    ++H    P        R+RGRQA  DLLM+ ER+RQ+EL  LL+ R VS F  R
Sbjct: 350 RV-GLAVNHEEGQPPHVRRDLRRVRGRQALLDLLMRAERERQRELQGLLEHRAVSDFAHR 409

Query: 336 GRIQSLLRLKILK--RGMAVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRS 395
            RIQSLLR + L+  R    E       + ++  RE Q   T+  LRE F     NG  +
Sbjct: 410 NRIQSLLRGRFLRNERPTVPERTPSMASRELLQLRERQ---TVSGLREGF----HNGREN 469

Query: 396 PIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQV-GASASASACVSDSDSAIHSSSMN 455
            + E   N +N D++ + + T+  A      ++ +V   S+++S   +DS     +S  N
Sbjct: 470 IVHE---NTSNTDNDNSNTSTNALAIAITAGNSQRVTDESSTSSRQGNDSPILPDNSESN 529

Query: 456 TVD-DHPIPEDVKEQTEDQGHDPPTSEPTWQDT--------PNLVLNSQDS--------I 515
             + D    ED  ++   Q + P    P  + T         N  +N  ++         
Sbjct: 530 LANADRDWEEDTNQRRIWQENVPVDERPNLEQTTLTQFDGYDNTDINRDETSVSDMHREA 589

Query: 516 HGWEESE-AAEENYGA--ESDYMGTSYNW---FTDISRPRSYWEDRRQSWYQQMLDSNSA 575
            G+ + E   +E +G   E+    +  NW    ++  R R   + RR + +    D N  
Sbjct: 590 SGFADDEYRTQEAHGVWHENSSRQSDGNWPGTRSEALRSRRVVQLRRLNRFHPPEDDNVY 649

Query: 576 NDEIRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELW 635
           + E+R+L+ R++VSN L S FRE +D+L+ S  E+R     +++                
Sbjct: 650 SMELRELLSRRSVSNLLHSGFRESLDQLIQSYAERRGHTHVDWD---------------- 709

Query: 636 CFSEGHTQPKSSSDNEEEEEEEEEDENSDYLDQSASPLQLASPSILSSWSYQDNEMGEDS 695
                + Q       E + + +    N + L+       L +P                 
Sbjct: 710 --LHANLQTAIPDSPERDTDHQVFVRNDNQLNGINGSQLLPTP----------------- 769

Query: 696 NRAASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSI 755
                  +P P P      +  S      H + IE E++ DLRG + +L Q MS++++ +
Sbjct: 770 ------PAPPPQPIWHQDLHHTSWSRHSMHRSEIEWEVMNDLRGDVARLQQGMSQMQRML 829

Query: 756 KCCMDMQLMLQQSIKQEVVG--GKIAGGRG----------RKSKSRKPKCCICHEMQIDS 802
           + CMDMQL LQ+S++QEV     + AG +G          R S   K  CC+C +  ID+
Sbjct: 830 EACMDMQLELQRSVRQEVSAALNRSAGDQGMSAETSEDGSRWSHVSKGTCCVCCDNHIDA 859