MS022474 (gene) Bitter gourd (TR) v1

Overview
NameMS022474
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionsec1 family domain-containing protein MIP3
Locationscaffold47: 3664098 .. 3668778 (-)
RNA-Seq ExpressionMS022474
SyntenyMS022474
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTGCTTGATGTCACAAGGTCATGCCTTGACTCCATTAACCAGGTAAGTCTATTAATAATAATTGTGGGTACCTTCTCTTTAATTTTGAACTTCAGCAAACCGTTCTTTTTTCTTTTTTTTTTCTTTGAAATCTTTTAGTCATTGATGCTGTTTCAGCTCCCCTCATTTCTGGTCGAGTTTGTGTTTTGGAAGTTTGCATTTTTCCATCTATCCAATGAAGGAGTTTTGTGTTTATTTTTATGAATTGGTTTTATAATCATACTTAAGACGTTTTGCTCAAAGGAGAAGTATGGGTGGTTCTTTCATGTTTTTTGTGGATATTTGATTATAATCCTTGCATCCTGGATGCCCATGCTAATTCATCGAAAGTGGTACTTTTTGGTACTGACACTTTAGGATTATTTGCCTTCACGCGCTGATTGTTAGGATGTGAAAGCTCGATGGTGTTCTATCTGTCCGGTTTATATAATGTTATCTCACTGTTTTGGCAGATATCAGATCACATTGAGGGCTCAATACTTTATCTCGATGCTGGGTGTGTGGAGAGTTTCCAAACTCTTGGGGGATTCCCGTTATTACTGGACCATGGTGTCCATGTTGTCTGTAGCTTGGAAAATCTGACTTCTCTTGATGCTGTAAGATAGTTGTTCTTGAGGTTTTACAGAAGGTTAAATGTTTCTCATCCTGCGTTTATATTGGAGTTTTTTTATTTTTTATTACCACCCCAAAAATTAAAGGGTAGAAATAAAACTTTTAAGTTCAATAATTTGACGAACAGAAACTTAGTGGTTGATTCACATAAACTATCCATGATTTAGTGGGTCGCATCTATTAGATTGTGTAAAATCTTCTGATTTACCAATGTTGTTTTATCATAAATTGTGGAAACCTCTTTTAGGTGGTTGATTGGAACCCAGCTTCTGCAAAGAAACTTGTGGTGATTACATCCCGTCTTCTAAGTGATGCACATCGTTATATTTTACGCTGCCTGACTGCGCATCAAGGTGTTCATCATTGTACCATATTTACATCTATCTCAGAGGTACTTCTTTTAAACTGTTTATTGTGCTTCATTTCCCTTGCATCTTGTATTTGCTTTGAATACGAGGTAGAACTAGGGTGCTATAACTGTGAAGACTTTAAACTTAAGCAATTAGATCCAAATAAGTAAATTTGATTAATAGCTTCTTCACTGCAATCAAATACTTTGCAGATTGCTCACTCTGCATACCCTGATTCACCTCTGGGACCAGATGCATACCATGAGTATGAATCTTTACTTATCCAAGATTATGAGGAGCTTGTTAAAAAAGGTGAAAAAAAAGCTGTACAGTCAGAAGACAGGAACTTGGAAAAGTATATATCCTCGGAAGATGAAGGATGGTCACGACTCACTTCTAGTGAAGAGGACATCACTCACCTGGAAGCTAGTTCAAGTGGAAGAGATTCGTATGAAGATATTCTGACGAGTCACCGAGAAGATGTAGGGCAAAAGCTCGTTGTTTCTGTGCATCACTTCCCCATGATTTTGTGTCCATTTTCACCAAGAGTTTTTGTGTTGCCTTCAGAGGGATTAATTGCTGAAGCATGCTTGTCAGCAGAAAGTGAGAATTCCCTTAGTCCTGGTTTGCCTCCCTTATACACTGGGTTGCCTCCTGATGGTGATGACGTTCCTCCTGGGGCAACTCTTACTGCACATTTTATTTACCATTTTGCTGCCAAGGTAATACCTCTGCACATGGTTAAGTCAAAATGTTTCCTATACTTGTTCCTTTTCATGGTTTTTATGTCCCGAGCTTCTATGGAAGTATGTTCTCTCTGTTTTTCGCTGAACAACATGAAAAAGGCTGAACCATAAGTTACTATTGAAGCTGAAGAAAGAAAAATCTTGTTAATACATCCATTGCCGAACTTTTTGTTTCTTTACTAAATTGCGTCTAAACTTTTGAAAACTAAAAATTGGTGTCTGTCTCATATACATGTTAATTCTTACATATATAAATATATTTCCTAAAAACCAGTAGAATGACTTTTACTAGACTTTTGTTAGAAGTCTAAGATCTGTTTTGTTTAGAATATGTAGCCTATTAGTTCTTTCCTATCATTACATGTTTTTGGTCTTTGAATGAATAGAGTAAAATCATTGATTTCAGATTTCACGAGTTCCAATTTCCATTTTCGTTTGGCCCTTTTTTCCAAGTTAAGAAATTTTTTTATCATGGGTTTCCATCTTGATAATTCTCTTCAATGCATTAAATTAAGTCTAGTGATTTAAACATCTCCTAGAGACCTTGCAAAGTCGATGCCCATTTCAGCAACAAAAAAAAAAAGTCGACTTTGACGTATCTATAATTGATTTATTTTTATTATTTTTTAAATGTATGAAATCTATAATAGAAATTCAACACATCTATACTAGATGAAAATAAAATGGTTATTTATTTTGTAGACTTGGATGAAAAGTTCATTTTTTCCTTGTAATATTGGCCATAATTATTCTTTCTCAATAATGTAGATGGACTTGAAGATGGAAATATTTTCCATTGGTGATCTGTCAAAAACTGTTGGAAAGATTTTGACAGATATGTCTAGTCTTTATGATGTAGGCCGACGCAAGAAATCAGCTGGTCTTTTACTAGTCGACCGCACTCTTGATCTTCTTACTCCATGTTGTCATGGCGACTCGCTTGTAGATCGCATGTTTTTGTCACTGCCTCGCAGAAAAAGAATGGCATCCTTTACCCATGTAAAAGGTCCAGAAACTTCCCTCAAACATGGTCCACCTATCTGTAGACGAGCGCCTCTTGATGTTCGGATACCATTAGCAGAAATTCTTACTGAAGATGGAAGTAAAGCTGATAAATTTCGGCTTGGAGAGAGAATTGAAGTTTTCCTGTCTGGATGGAATTCTGGAAACTCAACTTCCCAAAATATTGACAAGAGTGGTGAAAGCAACAGAGATCAAAATGGTCAATCACTATTTTATGACCCCGAGCTACTTAGTGGCTGTTTTGTCTCTTCCGAGAATTTTCGAGGAACTCCATACTTGGAAGCAATACTAGATAGGAAAACAAAAGATGGAGCTGTGCTGATAAAGAAGTGGCTGCAAGAAACTATGCGTAAGGAAAGCGTAGTTGTGAATGGGAAAATTCGACCTGGGGTTCCTTCCAAATTGGAGTTGGAATCTATTATCAAGGCACTGGCTAAAAGCCAGAGTTGTTTGTTGAAAAATAGAGGAATTCTTCAGCTAGCTGCTGCTGCAACAGTTGCACTCGAGGAATTAAACAGCGCTCGATGGGACGCCTTTCTTAGTGCAGAAAAAACATTGCGTGCAAGTGCTGAAGACACTACCCAGGGTCTGGCTGCACAAATTGTTGATCTTATAAACAAAAGTGTCTTGGTGGGTAAATTAGAATCTTCAAAGGGTGTCCTTTCATTTCAAGATTCTTTGCTCCTTACAATTACTGGTTATATATTGGCTGGCGATAATTTCCCAACATCTGGGCCCGATGGTCCGTTCTCTTGGCAAGAGGAGCATTTCATGAAAGAAGCTATTATAGATGCTATTTTAGAAAATCCAGTGGATGGAAGATTGAAGTTTCTCCATGGTTTAATAGAAGAGCTTGAGACGAACCGAGAAAGGATCAGACTGAAGGGATCAAAAGAGACGTCATCGAGCAAATTAAAAGAGGACGACTTTGACGATCAGTGGGAGAGCTGGGGTGATGAAGATGCTGATATTAACACTAATAATGAGGAAGTATATGATGACATGCAGCTGAAGTTGGAGTTGCGCGATCGGGTGGATAGTCTTTTCAAGTTACTTCACAAGTTGTCCAGTTCAAAGACGAGGAATTTACTGTTGAAGGAGACGTTAAATTCGGAAAATATGCTTCATGGTGATCAGTATGCAAATAAAGGAGTACTTTACAAGCTTCTGGCTAGAATCTTAAACAAGCATGATTTACCCAGTTTAGAATATCATTCCTCCACTATGGGGCGACTTTTCAAAAGCGGGTTTGGAAGATTTGGTCTTGGACAAGTAAGTTTTAATTCACGTAAGTGTTAAAACTAGATCTTTCTTATTCAAATGGTCTAAATTTTGGAGTTTGTTTTTCAGGCAAAACCTAGCCTTGCTGACCAAAATGTCATTCTAGTTTTTGTTCTGGGAGGCATCAATGGCCTTGAGGTATCTCTTTTACAAGAAAATGTAGCTAGCACTTTTATACACTTGATCTGTGAGTGAAATTAAAAATCTCTCTTTTTCAAAACACGCACCCACTTATTCTTTCTCTTTCGAGAAATTATTTAAAGGGTTGGAACAATGCAGCTGACACTTTATACTATTAGAGAAAAATTAAAACTTGTGTTCTGATTCTGCATTCGGTTCAATAGTTAGAAAGGTAGTCACATTGAATAATTTTTCTTCTCTTAACACGCACTTTTTCTCACTCACACACGCGCACATAAAATGCCATTCAAGTTGGTCGACAGATGGTCTCGAGTTGAAACATTCAAGCAAAACATGATGGTTATACTTATTTGCCAGGTTCGTGAAGCTCAGGAGGCATTGTCTGAGAATGGAAGACCAGATATAGAACTGATTATTGGTGGAACAACCTTCCTCACTCCTGATGCCATGTTTGATCTATTGCTTGGGGATTCAGCCTACGTT

mRNA sequence

ATGGCTTTGCTTGATGTCACAAGGTCATGCCTTGACTCCATTAACCAGATATCAGATCACATTGAGGGCTCAATACTTTATCTCGATGCTGGGTGTGTGGAGAGTTTCCAAACTCTTGGGGGATTCCCGTTATTACTGGACCATGGTGTCCATGTTGTCTGTAGCTTGGAAAATCTGACTTCTCTTGATGCTGTGGTTGATTGGAACCCAGCTTCTGCAAAGAAACTTGTGGTGATTACATCCCGTCTTCTAAGTGATGCACATCGTTATATTTTACGCTGCCTGACTGCGCATCAAGGTGTTCATCATTGTACCATATTTACATCTATCTCAGAGATTGCTCACTCTGCATACCCTGATTCACCTCTGGGACCAGATGCATACCATGAGTATGAATCTTTACTTATCCAAGATTATGAGGAGCTTGTTAAAAAAGGTGAAAAAAAAGCTGTACAGTCAGAAGACAGGAACTTGGAAAAGTATATATCCTCGGAAGATGAAGGATGGTCACGACTCACTTCTAGTGAAGAGGACATCACTCACCTGGAAGCTAGTTCAAGTGGAAGAGATTCGTATGAAGATATTCTGACGAGTCACCGAGAAGATGTAGGGCAAAAGCTCGTTGTTTCTGTGCATCACTTCCCCATGATTTTGTGTCCATTTTCACCAAGAGTTTTTGTGTTGCCTTCAGAGGGATTAATTGCTGAAGCATGCTTGTCAGCAGAAAGTGAGAATTCCCTTAGTCCTGGTTTGCCTCCCTTATACACTGGGTTGCCTCCTGATGGTGATGACGTTCCTCCTGGGGCAACTCTTACTGCACATTTTATTTACCATTTTGCTGCCAAGATGGACTTGAAGATGGAAATATTTTCCATTGGTGATCTGTCAAAAACTGTTGGAAAGATTTTGACAGATATGTCTAGTCTTTATGATGTAGGCCGACGCAAGAAATCAGCTGGTCTTTTACTAGTCGACCGCACTCTTGATCTTCTTACTCCATGTTGTCATGGCGACTCGCTTGTAGATCGCATGTTTTTGTCACTGCCTCGCAGAAAAAGAATGGCATCCTTTACCCATGTAAAAGGTCCAGAAACTTCCCTCAAACATGGTCCACCTATCTGTAGACGAGCGCCTCTTGATGTTCGGATACCATTAGCAGAAATTCTTACTGAAGATGGAAGTAAAGCTGATAAATTTCGGCTTGGAGAGAGAATTGAAGTTTTCCTGTCTGGATGGAATTCTGGAAACTCAACTTCCCAAAATATTGACAAGAGTGGTGAAAGCAACAGAGATCAAAATGGTCAATCACTATTTTATGACCCCGAGCTACTTAGTGGCTGTTTTGTCTCTTCCGAGAATTTTCGAGGAACTCCATACTTGGAAGCAATACTAGATAGGAAAACAAAAGATGGAGCTGTGCTGATAAAGAAGTGGCTGCAAGAAACTATGCGTAAGGAAAGCGTAGTTGTGAATGGGAAAATTCGACCTGGGGTTCCTTCCAAATTGGAGTTGGAATCTATTATCAAGGCACTGGCTAAAAGCCAGAGTTGTTTGTTGAAAAATAGAGGAATTCTTCAGCTAGCTGCTGCTGCAACAGTTGCACTCGAGGAATTAAACAGCGCTCGATGGGACGCCTTTCTTAGTGCAGAAAAAACATTGCGTGCAAGTGCTGAAGACACTACCCAGGGTCTGGCTGCACAAATTGTTGATCTTATAAACAAAAGTGTCTTGGTGGGTAAATTAGAATCTTCAAAGGGTGTCCTTTCATTTCAAGATTCTTTGCTCCTTACAATTACTGGTTATATATTGGCTGGCGATAATTTCCCAACATCTGGGCCCGATGGTCCGTTCTCTTGGCAAGAGGAGCATTTCATGAAAGAAGCTATTATAGATGCTATTTTAGAAAATCCAGTGGATGGAAGATTGAAGTTTCTCCATGGTTTAATAGAAGAGCTTGAGACGAACCGAGAAAGGATCAGACTGAAGGGATCAAAAGAGACGTCATCGAGCAAATTAAAAGAGGACGACTTTGACGATCAGTGGGAGAGCTGGGGTGATGAAGATGCTGATATTAACACTAATAATGAGGAAGTATATGATGACATGCAGCTGAAGTTGGAGTTGCGCGATCGGGTGGATAGTCTTTTCAAGTTACTTCACAAGTTGTCCAGTTCAAAGACGAGGAATTTACTGTTGAAGGAGACGTTAAATTCGGAAAATATGCTTCATGGTGATCAGTATGCAAATAAAGGAGTACTTTACAAGCTTCTGGCTAGAATCTTAAACAAGCATGATTTACCCAGTTTAGAATATCATTCCTCCACTATGGGGCGACTTTTCAAAAGCGGGTTTGGAAGATTTGGTCTTGGACAAGCAAAACCTAGCCTTGCTGACCAAAATGTCATTCTAGTTTTTGTTCTGGGAGGCATCAATGGCCTTGAGGTTCGTGAAGCTCAGGAGGCATTGTCTGAGAATGGAAGACCAGATATAGAACTGATTATTGGTGGAACAACCTTCCTCACTCCTGATGCCATGTTTGATCTATTGCTTGGGGATTCAGCCTACGTT

Coding sequence (CDS)

ATGGCTTTGCTTGATGTCACAAGGTCATGCCTTGACTCCATTAACCAGATATCAGATCACATTGAGGGCTCAATACTTTATCTCGATGCTGGGTGTGTGGAGAGTTTCCAAACTCTTGGGGGATTCCCGTTATTACTGGACCATGGTGTCCATGTTGTCTGTAGCTTGGAAAATCTGACTTCTCTTGATGCTGTGGTTGATTGGAACCCAGCTTCTGCAAAGAAACTTGTGGTGATTACATCCCGTCTTCTAAGTGATGCACATCGTTATATTTTACGCTGCCTGACTGCGCATCAAGGTGTTCATCATTGTACCATATTTACATCTATCTCAGAGATTGCTCACTCTGCATACCCTGATTCACCTCTGGGACCAGATGCATACCATGAGTATGAATCTTTACTTATCCAAGATTATGAGGAGCTTGTTAAAAAAGGTGAAAAAAAAGCTGTACAGTCAGAAGACAGGAACTTGGAAAAGTATATATCCTCGGAAGATGAAGGATGGTCACGACTCACTTCTAGTGAAGAGGACATCACTCACCTGGAAGCTAGTTCAAGTGGAAGAGATTCGTATGAAGATATTCTGACGAGTCACCGAGAAGATGTAGGGCAAAAGCTCGTTGTTTCTGTGCATCACTTCCCCATGATTTTGTGTCCATTTTCACCAAGAGTTTTTGTGTTGCCTTCAGAGGGATTAATTGCTGAAGCATGCTTGTCAGCAGAAAGTGAGAATTCCCTTAGTCCTGGTTTGCCTCCCTTATACACTGGGTTGCCTCCTGATGGTGATGACGTTCCTCCTGGGGCAACTCTTACTGCACATTTTATTTACCATTTTGCTGCCAAGATGGACTTGAAGATGGAAATATTTTCCATTGGTGATCTGTCAAAAACTGTTGGAAAGATTTTGACAGATATGTCTAGTCTTTATGATGTAGGCCGACGCAAGAAATCAGCTGGTCTTTTACTAGTCGACCGCACTCTTGATCTTCTTACTCCATGTTGTCATGGCGACTCGCTTGTAGATCGCATGTTTTTGTCACTGCCTCGCAGAAAAAGAATGGCATCCTTTACCCATGTAAAAGGTCCAGAAACTTCCCTCAAACATGGTCCACCTATCTGTAGACGAGCGCCTCTTGATGTTCGGATACCATTAGCAGAAATTCTTACTGAAGATGGAAGTAAAGCTGATAAATTTCGGCTTGGAGAGAGAATTGAAGTTTTCCTGTCTGGATGGAATTCTGGAAACTCAACTTCCCAAAATATTGACAAGAGTGGTGAAAGCAACAGAGATCAAAATGGTCAATCACTATTTTATGACCCCGAGCTACTTAGTGGCTGTTTTGTCTCTTCCGAGAATTTTCGAGGAACTCCATACTTGGAAGCAATACTAGATAGGAAAACAAAAGATGGAGCTGTGCTGATAAAGAAGTGGCTGCAAGAAACTATGCGTAAGGAAAGCGTAGTTGTGAATGGGAAAATTCGACCTGGGGTTCCTTCCAAATTGGAGTTGGAATCTATTATCAAGGCACTGGCTAAAAGCCAGAGTTGTTTGTTGAAAAATAGAGGAATTCTTCAGCTAGCTGCTGCTGCAACAGTTGCACTCGAGGAATTAAACAGCGCTCGATGGGACGCCTTTCTTAGTGCAGAAAAAACATTGCGTGCAAGTGCTGAAGACACTACCCAGGGTCTGGCTGCACAAATTGTTGATCTTATAAACAAAAGTGTCTTGGTGGGTAAATTAGAATCTTCAAAGGGTGTCCTTTCATTTCAAGATTCTTTGCTCCTTACAATTACTGGTTATATATTGGCTGGCGATAATTTCCCAACATCTGGGCCCGATGGTCCGTTCTCTTGGCAAGAGGAGCATTTCATGAAAGAAGCTATTATAGATGCTATTTTAGAAAATCCAGTGGATGGAAGATTGAAGTTTCTCCATGGTTTAATAGAAGAGCTTGAGACGAACCGAGAAAGGATCAGACTGAAGGGATCAAAAGAGACGTCATCGAGCAAATTAAAAGAGGACGACTTTGACGATCAGTGGGAGAGCTGGGGTGATGAAGATGCTGATATTAACACTAATAATGAGGAAGTATATGATGACATGCAGCTGAAGTTGGAGTTGCGCGATCGGGTGGATAGTCTTTTCAAGTTACTTCACAAGTTGTCCAGTTCAAAGACGAGGAATTTACTGTTGAAGGAGACGTTAAATTCGGAAAATATGCTTCATGGTGATCAGTATGCAAATAAAGGAGTACTTTACAAGCTTCTGGCTAGAATCTTAAACAAGCATGATTTACCCAGTTTAGAATATCATTCCTCCACTATGGGGCGACTTTTCAAAAGCGGGTTTGGAAGATTTGGTCTTGGACAAGCAAAACCTAGCCTTGCTGACCAAAATGTCATTCTAGTTTTTGTTCTGGGAGGCATCAATGGCCTTGAGGTTCGTGAAGCTCAGGAGGCATTGTCTGAGAATGGAAGACCAGATATAGAACTGATTATTGGTGGAACAACCTTCCTCACTCCTGATGCCATGTTTGATCTATTGCTTGGGGATTCAGCCTACGTT

Protein sequence

MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLTSLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPDSPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDITHLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLSAESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKTVGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFTHVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNSTSQNIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNSARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIRLKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLHKLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHSSTMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFLTPDAMFDLLLGDSAYV
Homology
BLAST of MS022474 vs. NCBI nr
Match: XP_022147932.1 (sec1 family domain-containing protein MIP3 [Momordica charantia] >XP_022147933.1 sec1 family domain-containing protein MIP3 [Momordica charantia])

HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 850/863 (98.49%), Postives = 852/863 (98.73%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT
Sbjct: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD
Sbjct: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AESENSLSP-------GLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIG 300
           AESENSLSP       GLPPLYTGLPPDGDDVPPGATLTAHF+YHFAAKMDLKMEIFSIG
Sbjct: 241 AESENSLSPGLPPLYTGLPPLYTGLPPDGDDVPPGATLTAHFLYHFAAKMDLKMEIFSIG 300

Query: 301 DLSKTVGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR 360
           DLSKTVGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR
Sbjct: 301 DLSKTVGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR 360

Query: 361 MASFTHVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWN 420
           MA FTHVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWN
Sbjct: 361 MAPFTHVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWN 420

Query: 421 SGNSTSQNIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAV 480
           SGNSTSQNIDKSGESNRDQNGQSLFYDPE+LSGCFVSSENFRGTPYLEAILDRKTKDGAV
Sbjct: 421 SGNSTSQNIDKSGESNRDQNGQSLFYDPEILSGCFVSSENFRGTPYLEAILDRKTKDGAV 480

Query: 481 LIKKWLQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATV 540
           LIKKWLQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATV
Sbjct: 481 LIKKWLQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATV 540

Query: 541 ALEELNSARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDS 600
           ALEELNS RWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDS
Sbjct: 541 ALEELNSTRWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDS 600

Query: 601 LLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELE 660
           LLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVD RLKFLHGLIEELE
Sbjct: 601 LLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDERLKFLHGLIEELE 660

Query: 661 TNRERIRLKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVD 720
           TNRERIRLKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVD
Sbjct: 661 TNRERIRLKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVD 720

Query: 721 SLFKLLHKLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHS 780
           SLFK LHKLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHS
Sbjct: 721 SLFKTLHKLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHS 780

Query: 781 STMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELI 840
           STMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELI
Sbjct: 781 STMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELI 840

Query: 841 IGGTTFLTPDAMFDLLLGDSAYV 857
           IGGTTFLTPDAMFDLLLGDSAYV
Sbjct: 841 IGGTTFLTPDAMFDLLLGDSAYV 863

BLAST of MS022474 vs. NCBI nr
Match: XP_038888123.1 (sec1 family domain-containing protein MIP3 [Benincasa hispida] >XP_038888124.1 sec1 family domain-containing protein MIP3 [Benincasa hispida])

HSP 1 Score: 1530.8 bits (3962), Expect = 0.0e+00
Identity = 768/856 (89.72%), Postives = 814/856 (95.09%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSI+QISDH+EGS+LYLDAGCVESFQ LGGFPLLLD GVHVVCSLEN+T
Sbjct: 1   MALLDVTRSCLDSISQISDHLEGSVLYLDAGCVESFQILGGFPLLLDLGVHVVCSLENMT 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           SLDAV+DWNPASAKKLVVITSRLLSDAHRYILRCLT HQGV HC IFTSISEIAHSAYPD
Sbjct: 61  SLDAVIDWNPASAKKLVVITSRLLSDAHRYILRCLTTHQGVRHCRIFTSISEIAHSAYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDA+HEYESLL+QDYEELVKKG KKAV SEDRNLEKYISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKGGKKAVPSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
            LEAS SGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASPSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKTVG 300
           AES +SLSPGLPPLY G+PPDGDD+PPGATLTAHF+YHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 AESVDSLSPGLPPLYIGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFTHV 360
           KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR +  +HV
Sbjct: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVSHV 360

Query: 361 KGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNSTSQ 420
           KGPETSL+HGP ICRRAPLDVRIPLAEILTEDG KADKFRLGERIEVFLSGWNSG+ST Q
Sbjct: 361 KGPETSLRHGPRICRRAPLDVRIPLAEILTEDGGKADKFRLGERIEVFLSGWNSGSSTPQ 420

Query: 421 NIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQ 480
           N DK GESNRDQN QS  YDPELLSGCFVSSENFRGT YLEAILDRKTKDG VLIKKWLQ
Sbjct: 421 NFDKGGESNRDQNLQSPIYDPELLSGCFVSSENFRGTSYLEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNS 540
           ETMRKESVVVNGKIRPG P+KLELES+IKAL+KSQ+CLL+N+G+LQLAAAATVA+EELNS
Sbjct: 481 ETMRKESVVVNGKIRPGFPTKLELESMIKALSKSQTCLLRNKGVLQLAAAATVAIEELNS 540

Query: 541 ARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITG 600
            RWDAFLSAEK LRASAEDT+QGLAAQIVDLINKSVL  K +SSKG+LSFQD+LLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLAVKSDSSKGILSFQDALLLTITG 600

Query: 601 YILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIR 660
           YILAG+NFPTSG DGPFSWQEEHFMKEAI DAILENPVDG+L FLHGLIEEL+TNR+RI+
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFMKEAITDAILENPVDGKLNFLHGLIEELQTNRDRIK 660

Query: 661 LKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLH 720
           LKG+K   S ++K+DDFDDQWESWGDEDADINT +EEVYDDMQLKLELRDRVD+LFK LH
Sbjct: 661 LKGTKVMGSMEIKDDDFDDQWESWGDEDADINTTSEEVYDDMQLKLELRDRVDNLFKTLH 720

Query: 721 KLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHSSTMGRLF 780
           KLS +K RNLLLKETLNSEN+L+GDQYANKGVLYKLLARILNKHDLP+LEYHSSTMGRLF
Sbjct: 721 KLSGTKKRNLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFL 840
           KSGFGRFGLGQAKP+LADQNVILVFV+GGINGLEVREAQEALSE+GRPDIELI+GGTTFL
Sbjct: 781 KSGFGRFGLGQAKPTLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840

Query: 841 TPDAMFDLLLGDSAYV 857
           TPD MFDLLLGDSAYV
Sbjct: 841 TPDDMFDLLLGDSAYV 856

BLAST of MS022474 vs. NCBI nr
Match: KAA0036925.1 (sec1 family domain-containing protein MIP3 [Cucumis melo var. makuwa] >TYK05547.1 sec1 family domain-containing protein MIP3 [Cucumis melo var. makuwa])

HSP 1 Score: 1516.1 bits (3924), Expect = 0.0e+00
Identity = 758/856 (88.55%), Postives = 814/856 (95.09%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSI+QI+DH++GSILYLDAGCVESFQ LGGFPLLLDHGV+VVCSLEN+ 
Sbjct: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           SLDAV+DWNPASA KLVVITSRLLSDAHRYILRCLT HQGV HCTIFTSISE+AHSAYPD
Sbjct: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQGVRHCTIFTSISELAHSAYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDA+HEYESLL+QDYEELVKK EKKA+ SEDRNLEKYISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKKALPSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
            LEASSSGR+SYEDILT+H+EDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASSSGRESYEDILTNHQEDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKTVG 300
           AES +SLSPGLPPLYTG+PPDGDD+PPGATLTAHF+YHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR +  THV
Sbjct: 301 KMLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360

Query: 361 KGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNSTSQ 420
           K PETSLK GP ICRRAP+DVRIP AEILTEDG KADKFRLGERIE FLSGWNSGNS SQ
Sbjct: 361 KSPETSLKKGPRICRRAPVDVRIPFAEILTEDGGKADKFRLGERIEAFLSGWNSGNSISQ 420

Query: 421 NIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQ 480
           N +KSGESNRDQ  QS  YDPELLSGCFVSSENFRGTPY+EAILDRKTKDG VLIKKWLQ
Sbjct: 421 NFNKSGESNRDQALQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNS 540
           ETMRKE+VVVNGKIRPG P+KLELES+IKALA+SQ+CLLKN+G+LQLAAAATVA+EELN+
Sbjct: 481 ETMRKENVVVNGKIRPGFPTKLELESMIKALARSQTCLLKNKGVLQLAAAATVAIEELNT 540

Query: 541 ARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITG 600
            RWDAFLSAEK LRASAEDT+QGLAAQIVDLINKSVLV K ESSKG+LSF+D+LLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSESSKGILSFEDALLLTITG 600

Query: 601 YILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIR 660
           YILAG+NFPTSG DGPFSWQEEHF+KEAIIDAILENPV G+LKFLHGLIEEL+TNR+RI+
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVGGKLKFLHGLIEELQTNRDRIK 660

Query: 661 LKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLH 720
            KG+K   SS++K+DDFDDQW+SWGD+DADINT NEEVYDDMQLKLELRDRVDSLFK LH
Sbjct: 661 SKGTKGIGSSQIKDDDFDDQWDSWGDDDADINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHSSTMGRLF 780
           KLS +K  NLLLKETLNSEN+L+GDQYANKGVLYKLLARILNKHDLP+LEYHSSTMGRLF
Sbjct: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFV+GG+NGLEVREAQEALSE+GRPDIELI+GGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGVNGLEVREAQEALSESGRPDIELIVGGTTFL 840

Query: 841 TPDAMFDLLLGDSAYV 857
           TPD MFDLLLGDSAYV
Sbjct: 841 TPDDMFDLLLGDSAYV 856

BLAST of MS022474 vs. NCBI nr
Match: XP_022952322.1 (sec1 family domain-containing protein MIP3 [Cucurbita moschata] >XP_022952323.1 sec1 family domain-containing protein MIP3 [Cucurbita moschata] >XP_022952324.1 sec1 family domain-containing protein MIP3 [Cucurbita moschata])

HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 761/856 (88.90%), Postives = 804/856 (93.93%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSI+QISDH+EGSILYLDAGCVESFQ LGGFPLLLDHGV VVCSLEN+T
Sbjct: 1   MALLDVTRSCLDSISQISDHLEGSILYLDAGCVESFQILGGFPLLLDHGVQVVCSLENMT 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           SLDAV+ WN AS KKLVV TSRLLSDAHRYILRCLTAHQGV HCTIFTSISE+AHSAYPD
Sbjct: 61  SLDAVIGWNLASTKKLVVFTSRLLSDAHRYILRCLTAHQGVRHCTIFTSISEVAHSAYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDA+HEYESLL+QDYEELVKKGEKK V SEDRN EKYISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKGEKKVVLSEDRNSEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           H+EASSSGR+SYE ILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGL+AEACLS
Sbjct: 181 HVEASSSGRNSYEGILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLVAEACLS 240

Query: 241 AESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKTVG 300
           AES +SLSPGLPPLYTG+PPD DD+PPGATLTAHF+YHFAAKMDLKMEIFSIGD+SKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDADDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDMSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVD MFLSLPRRKRMA  TH 
Sbjct: 301 KLLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDSMFLSLPRRKRMAPVTHA 360

Query: 361 KGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNSTSQ 420
           KGPE SLKH P ICRRAPLDVRIP AEILTEDG KADKFR+G RIE FLSGWNSGNS+SQ
Sbjct: 361 KGPEASLKHEPRICRRAPLDVRIPFAEILTEDGGKADKFRVGARIEAFLSGWNSGNSSSQ 420

Query: 421 NIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQ 480
           N DKSGESNRDQN QS  YDPELLSGCFVSSENFRGTP+LEAILDRK KD  VLIKKWLQ
Sbjct: 421 NFDKSGESNRDQNLQSPIYDPELLSGCFVSSENFRGTPFLEAILDRKIKDATVLIKKWLQ 480

Query: 481 ETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNS 540
           ETMRKESVVVNGKIR G P+KLELES+IKALAKSQSCLL+N+G+LQLAAAATVA+EE N 
Sbjct: 481 ETMRKESVVVNGKIRAGFPTKLELESMIKALAKSQSCLLRNKGVLQLAAAATVAIEESNR 540

Query: 541 ARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITG 600
            RWDAF SAEK LRASAEDT QGLAAQIVDLINKSVLVGK ESSKGVLSFQD+LLLTITG
Sbjct: 541 TRWDAFRSAEKVLRASAEDTIQGLAAQIVDLINKSVLVGKSESSKGVLSFQDALLLTITG 600

Query: 601 YILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIR 660
           YILAG+NFPTSG DGPFSWQEEHFMKEAI DAILENPV G LKFLHGL EEL+TNR+RI+
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFMKEAITDAILENPVGGELKFLHGLTEELQTNRDRIK 660

Query: 661 LKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLH 720
            KG+KETS S++K+DDFDDQWESWGDEDAD NT NEEVYDDMQLKLELRDRVDSLFK LH
Sbjct: 661 SKGTKETSLSEIKDDDFDDQWESWGDEDADNNTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHSSTMGRLF 780
           KLS +KTRNLLLKETLNSEN+L+GDQ+ANKGVLYKLLARILNKHDLP+LEYHSSTMGRLF
Sbjct: 721 KLSGTKTRNLLLKETLNSENILNGDQFANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALS++GRPDIELI+GGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSDSGRPDIELIVGGTTFL 840

Query: 841 TPDAMFDLLLGDSAYV 857
           TPD MFDLLLG+SAYV
Sbjct: 841 TPDDMFDLLLGESAYV 856

BLAST of MS022474 vs. NCBI nr
Match: XP_022968971.1 (sec1 family domain-containing protein MIP3 [Cucurbita maxima] >XP_022968972.1 sec1 family domain-containing protein MIP3 [Cucurbita maxima])

HSP 1 Score: 1509.6 bits (3907), Expect = 0.0e+00
Identity = 760/856 (88.79%), Postives = 803/856 (93.81%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSI+QISDH+EGSILYLDAGCVESFQ LGG PLLLDHGV VVCSLEN+T
Sbjct: 1   MALLDVTRSCLDSISQISDHLEGSILYLDAGCVESFQILGGLPLLLDHGVQVVCSLENMT 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           SLDAV+ WN ASAKKLVV TSRLLSDAHRYILRCLTAHQGV HCTIFTSISE+AHSAYPD
Sbjct: 61  SLDAVIGWNLASAKKLVVFTSRLLSDAHRYILRCLTAHQGVRHCTIFTSISEVAHSAYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDA+HEYESLL+QDYEELVKKGEKK V SEDRN  KYISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKGEKKVVLSEDRNSVKYISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           H+EASSSGR+SYE ILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGL+AEACLS
Sbjct: 181 HVEASSSGRNSYEGILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLVAEACLS 240

Query: 241 AESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKTVG 300
           AES +SLSPGLPPLYTG+PPDGDD+PPGATLTAHF+YHFAAKMDLKMEIFSIGD+SKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDMSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVD MFLSLPRRKRMA  TH 
Sbjct: 301 KLLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDSMFLSLPRRKRMAPVTHA 360

Query: 361 KGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNSTSQ 420
           KGPE SLKH P ICRRAPLDVRIP AEILTEDG KADKFRLG RIE FLSGWNSGNS+SQ
Sbjct: 361 KGPEASLKHEPRICRRAPLDVRIPFAEILTEDGGKADKFRLGARIEAFLSGWNSGNSSSQ 420

Query: 421 NIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQ 480
           N DKSGESNRDQN QS  YDPELLSGCFVSSENFRGTP+LEAILDRK KD  VLIKKWLQ
Sbjct: 421 NFDKSGESNRDQNLQSPIYDPELLSGCFVSSENFRGTPFLEAILDRKIKDATVLIKKWLQ 480

Query: 481 ETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNS 540
           ETMRKESVVVNGKIR G P+KLELES+IKALAKSQSCLL+N+G+LQLAAAATVA+EE N 
Sbjct: 481 ETMRKESVVVNGKIRAGFPTKLELESMIKALAKSQSCLLRNKGVLQLAAAATVAIEESNR 540

Query: 541 ARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITG 600
            RWDAF SAEK LRASAEDT QGLAAQIVDLINKSVLVGK ESSKGVLSFQD+LLLTITG
Sbjct: 541 TRWDAFRSAEKVLRASAEDTIQGLAAQIVDLINKSVLVGKSESSKGVLSFQDALLLTITG 600

Query: 601 YILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIR 660
           YILAG+NFPTSG DGPFSWQEEHFMKEAI DAILENPV G LKFLHGL EEL+TNR+RI+
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFMKEAITDAILENPVGGELKFLHGLTEELQTNRDRIK 660

Query: 661 LKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLH 720
            KG+K  S S++K+DDFDDQWESWGDEDAD NT NEEVYDDMQLKLELRDRVDSLFK+LH
Sbjct: 661 SKGTKAMSLSEIKDDDFDDQWESWGDEDADNNTTNEEVYDDMQLKLELRDRVDSLFKMLH 720

Query: 721 KLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHSSTMGRLF 780
           KLS +KTRNLLLKETLNSEN+L+GDQ+ANKGVLYKLLARILNKHDLP+LEYHSSTMGRLF
Sbjct: 721 KLSGTKTRNLLLKETLNSENILNGDQFANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALS++GRPDIELI+GGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSDSGRPDIELIVGGTTFL 840

Query: 841 TPDAMFDLLLGDSAYV 857
           TPD MFDLLLG+SAYV
Sbjct: 841 TPDDMFDLLLGESAYV 856

BLAST of MS022474 vs. ExPASy Swiss-Prot
Match: F4IP69 (Sec1 family domain-containing protein MIP3 OS=Arabidopsis thaliana OX=3702 GN=MIP3 PE=1 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 1.1e-278
Identity = 513/871 (58.90%), Postives = 645/871 (74.05%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MAL+DV  SCL+SI +I + ++ +I+Y+DAGC ESFQ +G FPL L+ G   VCSLEN+T
Sbjct: 1   MALIDVAISCLNSIREIEEDVKDAIVYIDAGCTESFQFVGAFPLFLELGARAVCSLENMT 60

Query: 61  SLDAVVDWNPAS--AKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAY 120
           SLDAV DWN  S  AK++V++TSRLL+DAHRY+LRCL+ H+GV  CT+FTSISE +HSA 
Sbjct: 61  SLDAVADWNSKSDCAKRIVIMTSRLLNDAHRYMLRCLSTHEGVQRCTVFTSISEGSHSAI 120

Query: 121 PDSPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEED 180
           PDSPLGPDAY EYE+LL+QDY E  KK +K    S+D+ + K+ S+ +     LT    +
Sbjct: 121 PDSPLGPDAYREYETLLVQDYNEHTKKSDK---ISKDKGVSKFSSALES----LTMEPIE 180

Query: 181 ITHLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEAC 240
             +++ SS G                Q LVVSVHHFP+I+CPF+PR FVLPS+G +AEA 
Sbjct: 181 SENVDISSGG---------------AQGLVVSVHHFPLIICPFTPRAFVLPSQGSVAEAS 240

Query: 241 LSAESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKT 300
           LS + E+SLS GLPP+ TG   D DDVPPGATLTAHF+Y  A KM+LK+EIFS+GD SK 
Sbjct: 241 LSRQHEDSLSFGLPPISTGSMSDTDDVPPGATLTAHFLYQLALKMELKLEIFSLGDQSKN 300

Query: 301 VGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFT 360
           VGKILTDMSS+YDV RRK+SAGLLLVDRTLDL+TPCCHGDSL DR+F SLPR +R +S  
Sbjct: 301 VGKILTDMSSVYDVARRKRSAGLLLVDRTLDLITPCCHGDSLFDRIFSSLPRAERFSS-- 360

Query: 361 HVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNST 420
                +  LK G P   R  LDV++PL E+L E+ SK     L E IE FL GW+S  S 
Sbjct: 361 -----QAQLKQGVPSINRPSLDVQVPLGELLNEEPSKIRDSGLPEGIEAFLRGWDSYTSA 420

Query: 421 SQNIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKW 480
            QN+    E ++    +S     ELL+G  V++E FRGTPYLEA++DRKTKDG+VL+KKW
Sbjct: 421 PQNVGLFNECDK----KSTTNWTELLNGSLVATECFRGTPYLEAMIDRKTKDGSVLVKKW 480

Query: 481 LQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEEL 540
           LQE +R+E++ VN + RPG  +K EL+++IKAL++SQS LLKN+GI+QL AA   AL+E 
Sbjct: 481 LQEALRRENISVNVRARPGYATKPELQAMIKALSQSQSSLLKNKGIIQLGAATAAALDES 540

Query: 541 NSARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLE---------SSKGVLS 600
            SA+WD F SAE  L  SA DT+QGLAAQI DLINKS  V +L+         SS+G+LS
Sbjct: 541 QSAKWDTFSSAEMMLNVSAGDTSQGLAAQISDLINKSA-VAELQAKKNEKPDSSSRGLLS 600

Query: 601 FQDSLLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLI 660
           F+D+LLLTI GYILAG+NFPTSG  GPFSWQEEHF+KEAI+DA+LENP  G LKFL+GL 
Sbjct: 601 FRDALLLTIVGYILAGENFPTSGSGGPFSWQEEHFLKEAIVDAVLENPSAGNLKFLNGLT 660

Query: 661 EELETNRERIRLKGSKETSSSKLKEDDF--DDQWESWGDEDAD--INTNNEEVYDDMQLK 720
           EELE    R++ + +KE  S    + D   DD W  WGDE+ +   N+  +E YDDMQLK
Sbjct: 661 EELEGRLNRLKSEETKEIPSDDQLDIDALDDDPWGKWGDEEEEEVDNSKADESYDDMQLK 720

Query: 721 LELRDRVDSLFKLLHKLSSSKTRNLLLKE-TLNSENMLHGDQYANKGVLYKLLARILNKH 780
           L+LRDRVDSLF+ LHKLSS +TRNL L+E +L SE+   G+   NKG++Y+L+ ++L+K 
Sbjct: 721 LDLRDRVDSLFRFLHKLSSLRTRNLPLREGSLASESSFPGEPSGNKGLVYRLITKVLSKQ 780

Query: 781 DLPSLEYHSSTMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSE 840
           ++P LEYHSST+GR  KSGFGRFGLGQAKPSLADQ+VILVFV+GGING+EV EAQEA+SE
Sbjct: 781 EIPGLEYHSSTVGRFIKSGFGRFGLGQAKPSLADQSVILVFVIGGINGIEVLEAQEAVSE 837

Query: 841 NGRPDIELIIGGTTFLTPDAMFDLLLGDSAY 856
           +GRPDI L+IGGTT LTPD MF+LLLG  ++
Sbjct: 841 SGRPDINLVIGGTTLLTPDDMFELLLGQFSH 837

BLAST of MS022474 vs. ExPASy Swiss-Prot
Match: Q8BTY8 (Sec1 family domain-containing protein 2 OS=Mus musculus OX=10090 GN=Scfd2 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.1e-10
Identity = 92/412 (22.33%), Postives = 173/412 (41.99%), Query Frame = 0

Query: 446 GCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQETMRKESVVVN---GKIRPGVPSKL 505
           GC   S         EA+L+ K K+  + +++ L E   +E++ +    G++ PG     
Sbjct: 330 GCLAPSNEASAKALWEALLNSKHKEAVMEVRRHLVEAASRENLPIKMSMGRVTPG----- 389

Query: 506 ELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNSARWDAFLSAEKTLRASAEDTTQ 565
           +L S I+    +   L  + G+LQL  A    L+   +A+WD FL+ E+ L  S  D+T 
Sbjct: 390 QLMSYIQLFKNNLRALRNHCGLLQLGMATVQTLKHPQTAKWDNFLAFERLLLQSLGDSTM 449

Query: 566 -GLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITGYILAGDNFPTSGPDGPFSWQE 625
            G+  Q++ +I  S      + +   L+ ++ L+L I  Y + GD               
Sbjct: 450 AGVLNQLLPMIKSS-----SQRTSDDLNPEELLILLIYIYSVPGD--------------- 509

Query: 626 EHFMKEAIIDAILENPVDGRLK--FLHGLIEELETNRERIRLKGSKETSSSKLKEDDFDD 685
                   +D  L   V+ ++K    H L EE E +    ++ G                
Sbjct: 510 ------VTLDRDL-GDVEEKVKKALAHVLSEESELSPLLQKITGC--------------- 569

Query: 686 QWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLHKLSSSKTRNLLLKETLNSE 745
                 D   D+     ++             V+ +F  L +++ ++      K      
Sbjct: 570 ------DSAVDLTLPKSQI------------AVNDVFMALREIAGARNLMRQFKSVYVPG 629

Query: 746 NMLHGDQYANKGVLYKLLARILN--KHDLPSLEYHSSTMGRLFKSGFGRFGLGQAKPSLA 805
           N  H   Y  K +L +++  I N  K D   +E+ SS +  L K+GF  F +  ++P  +
Sbjct: 630 NNTHQASY--KPLLKQVVEEIFNPEKSDPIDIEHMSSGLTDLLKTGFSMF-MKVSRPHPS 672

Query: 806 DQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFLTPDAMFDLL 850
           D  ++++FV+GG+   E +  ++ L  + +P  ++++  T  L P  + +LL
Sbjct: 690 DHPLLILFVVGGVTVAEAKMVKD-LVASLKPGTQVMVLSTRLLKPLNIPELL 672

BLAST of MS022474 vs. ExPASy Swiss-Prot
Match: Q8WU76 (Sec1 family domain-containing protein 2 OS=Homo sapiens OX=9606 GN=SCFD2 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 3.4e-09
Identity = 95/409 (23.23%), Postives = 173/409 (42.30%), Query Frame = 0

Query: 446 GCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQETMRKESVVVN---GKIRPGVPSKL 505
           GC   S +       EA+L+ K K+  + +++ L E   +E++ +    G++ PG     
Sbjct: 330 GCLSQSSDTTAKALWEALLNTKHKEAVMEVRRHLVEAASRENLPIKMSMGRVTPG----- 389

Query: 506 ELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNSARWDAFLSAEKTLRASAEDTTQ 565
           +L S I+    +   L+ + G+LQL  A    L+   +A+WD FL+ E+ L  S      
Sbjct: 390 QLMSYIQLFKNNLKALMNHCGLLQLGLATAQTLKHPQTAKWDNFLAFERLLLQS------ 449

Query: 566 GLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITGYILAGDNFPTSGPDGPFSWQEE 625
                          +G  ES+  V+  Q   LL +   +    N             E+
Sbjct: 450 ---------------IG--ESAMSVVLNQ---LLPMIKPVTQRTN-------------ED 509

Query: 626 HFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIRLKGSKETSSSKLKEDDFDDQWE 685
           +  +E +I  I    V G L     L E  E  ++ +     +E+  S L +   D    
Sbjct: 510 YSPEELLILLIYIYSVTGELTVDKDLCEAEEKVKKALAQVFCEESGLSPLLQKITD---- 569

Query: 686 SWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLHKLSSSKTRNLLLKETLNSENML 745
            W   D+ IN              + +  VD LF  L  ++ +++     K      N  
Sbjct: 570 -W---DSSINLTFH----------KSKIAVDELFTSLRDIAGARSLLKQFKSVYVPGNHT 629

Query: 746 HGDQYANKGVLYKLLARILN--KHDLPSLEYHSSTMGRLFKSGFGRFGLGQAKPSLADQN 805
           H   Y  K +L +++  I +  + D   +E+ SS +  L K+GF  F +  ++P  +D  
Sbjct: 630 HQASY--KPLLKQVVEEIFHPERPDSVDIEHMSSGLTDLLKTGFSMF-MKVSRPHPSDYP 672

Query: 806 VILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFLTPDAMFDLL 850
           ++++FV+GG+   EV+  ++ L  + +P  ++I+  T  L P  + +LL
Sbjct: 690 LLILFVVGGVTVSEVKMVKD-LVASLKPGTQVIVLSTRLLKPLNIPELL 672

BLAST of MS022474 vs. ExPASy TrEMBL
Match: A0A6J1D2N5 (sec1 family domain-containing protein MIP3 OS=Momordica charantia OX=3673 GN=LOC111016742 PE=3 SV=1)

HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 850/863 (98.49%), Postives = 852/863 (98.73%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT
Sbjct: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD
Sbjct: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AESENSLSP-------GLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIG 300
           AESENSLSP       GLPPLYTGLPPDGDDVPPGATLTAHF+YHFAAKMDLKMEIFSIG
Sbjct: 241 AESENSLSPGLPPLYTGLPPLYTGLPPDGDDVPPGATLTAHFLYHFAAKMDLKMEIFSIG 300

Query: 301 DLSKTVGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR 360
           DLSKTVGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR
Sbjct: 301 DLSKTVGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR 360

Query: 361 MASFTHVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWN 420
           MA FTHVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWN
Sbjct: 361 MAPFTHVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWN 420

Query: 421 SGNSTSQNIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAV 480
           SGNSTSQNIDKSGESNRDQNGQSLFYDPE+LSGCFVSSENFRGTPYLEAILDRKTKDGAV
Sbjct: 421 SGNSTSQNIDKSGESNRDQNGQSLFYDPEILSGCFVSSENFRGTPYLEAILDRKTKDGAV 480

Query: 481 LIKKWLQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATV 540
           LIKKWLQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATV
Sbjct: 481 LIKKWLQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATV 540

Query: 541 ALEELNSARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDS 600
           ALEELNS RWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDS
Sbjct: 541 ALEELNSTRWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDS 600

Query: 601 LLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELE 660
           LLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVD RLKFLHGLIEELE
Sbjct: 601 LLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDERLKFLHGLIEELE 660

Query: 661 TNRERIRLKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVD 720
           TNRERIRLKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVD
Sbjct: 661 TNRERIRLKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVD 720

Query: 721 SLFKLLHKLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHS 780
           SLFK LHKLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHS
Sbjct: 721 SLFKTLHKLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHS 780

Query: 781 STMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELI 840
           STMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELI
Sbjct: 781 STMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELI 840

Query: 841 IGGTTFLTPDAMFDLLLGDSAYV 857
           IGGTTFLTPDAMFDLLLGDSAYV
Sbjct: 841 IGGTTFLTPDAMFDLLLGDSAYV 863

BLAST of MS022474 vs. ExPASy TrEMBL
Match: A0A5A7T609 (Sec1 family domain-containing protein MIP3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold178G00550 PE=3 SV=1)

HSP 1 Score: 1516.1 bits (3924), Expect = 0.0e+00
Identity = 758/856 (88.55%), Postives = 814/856 (95.09%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSI+QI+DH++GSILYLDAGCVESFQ LGGFPLLLDHGV+VVCSLEN+ 
Sbjct: 1   MALLDVTRSCLDSISQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           SLDAV+DWNPASA KLVVITSRLLSDAHRYILRCLT HQGV HCTIFTSISE+AHSAYPD
Sbjct: 61  SLDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQGVRHCTIFTSISELAHSAYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDA+HEYESLL+QDYEELVKK EKKA+ SEDRNLEKYISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKKALPSEDRNLEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
            LEASSSGR+SYEDILT+H+EDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASSSGRESYEDILTNHQEDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKTVG 300
           AES +SLSPGLPPLYTG+PPDGDD+PPGATLTAHF+YHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR +  THV
Sbjct: 301 KMLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360

Query: 361 KGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNSTSQ 420
           K PETSLK GP ICRRAP+DVRIP AEILTEDG KADKFRLGERIE FLSGWNSGNS SQ
Sbjct: 361 KSPETSLKKGPRICRRAPVDVRIPFAEILTEDGGKADKFRLGERIEAFLSGWNSGNSISQ 420

Query: 421 NIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQ 480
           N +KSGESNRDQ  QS  YDPELLSGCFVSSENFRGTPY+EAILDRKTKDG VLIKKWLQ
Sbjct: 421 NFNKSGESNRDQALQSPIYDPELLSGCFVSSENFRGTPYMEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNS 540
           ETMRKE+VVVNGKIRPG P+KLELES+IKALA+SQ+CLLKN+G+LQLAAAATVA+EELN+
Sbjct: 481 ETMRKENVVVNGKIRPGFPTKLELESMIKALARSQTCLLKNKGVLQLAAAATVAIEELNT 540

Query: 541 ARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITG 600
            RWDAFLSAEK LRASAEDT+QGLAAQIVDLINKSVLV K ESSKG+LSF+D+LLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSESSKGILSFEDALLLTITG 600

Query: 601 YILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIR 660
           YILAG+NFPTSG DGPFSWQEEHF+KEAIIDAILENPV G+LKFLHGLIEEL+TNR+RI+
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVGGKLKFLHGLIEELQTNRDRIK 660

Query: 661 LKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLH 720
            KG+K   SS++K+DDFDDQW+SWGD+DADINT NEEVYDDMQLKLELRDRVDSLFK LH
Sbjct: 661 SKGTKGIGSSQIKDDDFDDQWDSWGDDDADINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHSSTMGRLF 780
           KLS +K  NLLLKETLNSEN+L+GDQYANKGVLYKLLARILNKHDLP+LEYHSSTMGRLF
Sbjct: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFV+GG+NGLEVREAQEALSE+GRPDIELI+GGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGVNGLEVREAQEALSESGRPDIELIVGGTTFL 840

Query: 841 TPDAMFDLLLGDSAYV 857
           TPD MFDLLLGDSAYV
Sbjct: 841 TPDDMFDLLLGDSAYV 856

BLAST of MS022474 vs. ExPASy TrEMBL
Match: A0A6J1GK80 (sec1 family domain-containing protein MIP3 OS=Cucurbita moschata OX=3662 GN=LOC111455034 PE=3 SV=1)

HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 761/856 (88.90%), Postives = 804/856 (93.93%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSI+QISDH+EGSILYLDAGCVESFQ LGGFPLLLDHGV VVCSLEN+T
Sbjct: 1   MALLDVTRSCLDSISQISDHLEGSILYLDAGCVESFQILGGFPLLLDHGVQVVCSLENMT 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           SLDAV+ WN AS KKLVV TSRLLSDAHRYILRCLTAHQGV HCTIFTSISE+AHSAYPD
Sbjct: 61  SLDAVIGWNLASTKKLVVFTSRLLSDAHRYILRCLTAHQGVRHCTIFTSISEVAHSAYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDA+HEYESLL+QDYEELVKKGEKK V SEDRN EKYISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKGEKKVVLSEDRNSEKYISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           H+EASSSGR+SYE ILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGL+AEACLS
Sbjct: 181 HVEASSSGRNSYEGILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLVAEACLS 240

Query: 241 AESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKTVG 300
           AES +SLSPGLPPLYTG+PPD DD+PPGATLTAHF+YHFAAKMDLKMEIFSIGD+SKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDADDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDMSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVD MFLSLPRRKRMA  TH 
Sbjct: 301 KLLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDSMFLSLPRRKRMAPVTHA 360

Query: 361 KGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNSTSQ 420
           KGPE SLKH P ICRRAPLDVRIP AEILTEDG KADKFR+G RIE FLSGWNSGNS+SQ
Sbjct: 361 KGPEASLKHEPRICRRAPLDVRIPFAEILTEDGGKADKFRVGARIEAFLSGWNSGNSSSQ 420

Query: 421 NIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQ 480
           N DKSGESNRDQN QS  YDPELLSGCFVSSENFRGTP+LEAILDRK KD  VLIKKWLQ
Sbjct: 421 NFDKSGESNRDQNLQSPIYDPELLSGCFVSSENFRGTPFLEAILDRKIKDATVLIKKWLQ 480

Query: 481 ETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNS 540
           ETMRKESVVVNGKIR G P+KLELES+IKALAKSQSCLL+N+G+LQLAAAATVA+EE N 
Sbjct: 481 ETMRKESVVVNGKIRAGFPTKLELESMIKALAKSQSCLLRNKGVLQLAAAATVAIEESNR 540

Query: 541 ARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITG 600
            RWDAF SAEK LRASAEDT QGLAAQIVDLINKSVLVGK ESSKGVLSFQD+LLLTITG
Sbjct: 541 TRWDAFRSAEKVLRASAEDTIQGLAAQIVDLINKSVLVGKSESSKGVLSFQDALLLTITG 600

Query: 601 YILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIR 660
           YILAG+NFPTSG DGPFSWQEEHFMKEAI DAILENPV G LKFLHGL EEL+TNR+RI+
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFMKEAITDAILENPVGGELKFLHGLTEELQTNRDRIK 660

Query: 661 LKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLH 720
            KG+KETS S++K+DDFDDQWESWGDEDAD NT NEEVYDDMQLKLELRDRVDSLFK LH
Sbjct: 661 SKGTKETSLSEIKDDDFDDQWESWGDEDADNNTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHSSTMGRLF 780
           KLS +KTRNLLLKETLNSEN+L+GDQ+ANKGVLYKLLARILNKHDLP+LEYHSSTMGRLF
Sbjct: 721 KLSGTKTRNLLLKETLNSENILNGDQFANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALS++GRPDIELI+GGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSDSGRPDIELIVGGTTFL 840

Query: 841 TPDAMFDLLLGDSAYV 857
           TPD MFDLLLG+SAYV
Sbjct: 841 TPDDMFDLLLGESAYV 856

BLAST of MS022474 vs. ExPASy TrEMBL
Match: A0A6J1HV02 (sec1 family domain-containing protein MIP3 OS=Cucurbita maxima OX=3661 GN=LOC111468119 PE=3 SV=1)

HSP 1 Score: 1509.6 bits (3907), Expect = 0.0e+00
Identity = 760/856 (88.79%), Postives = 803/856 (93.81%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSI+QISDH+EGSILYLDAGCVESFQ LGG PLLLDHGV VVCSLEN+T
Sbjct: 1   MALLDVTRSCLDSISQISDHLEGSILYLDAGCVESFQILGGLPLLLDHGVQVVCSLENMT 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           SLDAV+ WN ASAKKLVV TSRLLSDAHRYILRCLTAHQGV HCTIFTSISE+AHSAYPD
Sbjct: 61  SLDAVIGWNLASAKKLVVFTSRLLSDAHRYILRCLTAHQGVRHCTIFTSISEVAHSAYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDA+HEYESLL+QDYEELVKKGEKK V SEDRN  KYISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKGEKKVVLSEDRNSVKYISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
           H+EASSSGR+SYE ILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGL+AEACLS
Sbjct: 181 HVEASSSGRNSYEGILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLVAEACLS 240

Query: 241 AESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKTVG 300
           AES +SLSPGLPPLYTG+PPDGDD+PPGATLTAHF+YHFAAKMDLKMEIFSIGD+SKTVG
Sbjct: 241 AESVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDMSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFTHV 360
           K+LTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVD MFLSLPRRKRMA  TH 
Sbjct: 301 KLLTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDSMFLSLPRRKRMAPVTHA 360

Query: 361 KGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNSTSQ 420
           KGPE SLKH P ICRRAPLDVRIP AEILTEDG KADKFRLG RIE FLSGWNSGNS+SQ
Sbjct: 361 KGPEASLKHEPRICRRAPLDVRIPFAEILTEDGGKADKFRLGARIEAFLSGWNSGNSSSQ 420

Query: 421 NIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQ 480
           N DKSGESNRDQN QS  YDPELLSGCFVSSENFRGTP+LEAILDRK KD  VLIKKWLQ
Sbjct: 421 NFDKSGESNRDQNLQSPIYDPELLSGCFVSSENFRGTPFLEAILDRKIKDATVLIKKWLQ 480

Query: 481 ETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNS 540
           ETMRKESVVVNGKIR G P+KLELES+IKALAKSQSCLL+N+G+LQLAAAATVA+EE N 
Sbjct: 481 ETMRKESVVVNGKIRAGFPTKLELESMIKALAKSQSCLLRNKGVLQLAAAATVAIEESNR 540

Query: 541 ARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITG 600
            RWDAF SAEK LRASAEDT QGLAAQIVDLINKSVLVGK ESSKGVLSFQD+LLLTITG
Sbjct: 541 TRWDAFRSAEKVLRASAEDTIQGLAAQIVDLINKSVLVGKSESSKGVLSFQDALLLTITG 600

Query: 601 YILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIR 660
           YILAG+NFPTSG DGPFSWQEEHFMKEAI DAILENPV G LKFLHGL EEL+TNR+RI+
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFMKEAITDAILENPVGGELKFLHGLTEELQTNRDRIK 660

Query: 661 LKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLH 720
            KG+K  S S++K+DDFDDQWESWGDEDAD NT NEEVYDDMQLKLELRDRVDSLFK+LH
Sbjct: 661 SKGTKAMSLSEIKDDDFDDQWESWGDEDADNNTTNEEVYDDMQLKLELRDRVDSLFKMLH 720

Query: 721 KLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHSSTMGRLF 780
           KLS +KTRNLLLKETLNSEN+L+GDQ+ANKGVLYKLLARILNKHDLP+LEYHSSTMGRLF
Sbjct: 721 KLSGTKTRNLLLKETLNSENILNGDQFANKGVLYKLLARILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALS++GRPDIELI+GGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSDSGRPDIELIVGGTTFL 840

Query: 841 TPDAMFDLLLGDSAYV 857
           TPD MFDLLLG+SAYV
Sbjct: 841 TPDDMFDLLLGESAYV 856

BLAST of MS022474 vs. ExPASy TrEMBL
Match: A0A0A0LYK3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G538250 PE=3 SV=1)

HSP 1 Score: 1500.7 bits (3884), Expect = 0.0e+00
Identity = 754/856 (88.08%), Postives = 805/856 (94.04%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MALLDVTRSCLDSI QI+DH++GSILYLDAGCVESFQ LGGFPLLLDHGV+VVCSLEN+ 
Sbjct: 1   MALLDVTRSCLDSITQIADHLKGSILYLDAGCVESFQILGGFPLLLDHGVNVVCSLENMA 60

Query: 61  SLDAVVDWNPASAKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAYPD 120
           +LDAV+DWNPASA KLVVITSRLLSDAHRYILRCLT HQ V HCTIFTSISEIAHS YPD
Sbjct: 61  ALDAVIDWNPASATKLVVITSRLLSDAHRYILRCLTTHQDVRHCTIFTSISEIAHSVYPD 120

Query: 121 SPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEEDIT 180
           SPLGPDA+HEYESLL+QDYEELVKK EKKA+ SEDR LEK ISSEDEGWSRLTSSEEDIT
Sbjct: 121 SPLGPDAFHEYESLLVQDYEELVKKDEKKALPSEDRILEKCISSEDEGWSRLTSSEEDIT 180

Query: 181 HLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240
            LEASSSGRDSYED+LTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS
Sbjct: 181 QLEASSSGRDSYEDVLTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEACLS 240

Query: 241 AESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKTVG 300
            E+ +SLSPGLPPLYTG+PPDGDD+PPGATLTAHF+YHFAAKMDLKMEIFSIGDLSKTVG
Sbjct: 241 PENVDSLSPGLPPLYTGMPPDGDDIPPGATLTAHFLYHFAAKMDLKMEIFSIGDLSKTVG 300

Query: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFTHV 360
           KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKR +  THV
Sbjct: 301 KILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRTSPVTHV 360

Query: 361 KGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNSTSQ 420
           KGPETSLK GP ICRRAPLDVRIP AEILTED  KADKFRLGERIE FLSGWNS NSTSQ
Sbjct: 361 KGPETSLKKGPRICRRAPLDVRIPFAEILTEDEGKADKFRLGERIEAFLSGWNSRNSTSQ 420

Query: 421 NIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKWLQ 480
           N + SGESNRDQ  QS  YDPELLSGCFVSSENFRG PY+EAILDRKTKDG VLIKKWLQ
Sbjct: 421 NFNNSGESNRDQTLQSPIYDPELLSGCFVSSENFRGIPYMEAILDRKTKDGTVLIKKWLQ 480

Query: 481 ETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEELNS 540
           ETMRKE+VVVNGKIRPG P+K+ELES+IKALAKSQ+C L+N+G+LQLAAAATVA+EELNS
Sbjct: 481 ETMRKENVVVNGKIRPGFPTKVELESMIKALAKSQTCWLRNKGVLQLAAAATVAIEELNS 540

Query: 541 ARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLESSKGVLSFQDSLLLTITG 600
            RWDAFLSAEK LRASAEDT+QGLAAQIVDLINKSVLV K E+SKGVLSF+D+LLLTITG
Sbjct: 541 TRWDAFLSAEKILRASAEDTSQGLAAQIVDLINKSVLVVKSEASKGVLSFEDALLLTITG 600

Query: 601 YILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLIEELETNRERIR 660
           YILAG+NFPTSG DGPFSWQEEHF+KEAIIDAILENPVDG+LKFLHGLIEEL+TNR+R++
Sbjct: 601 YILAGENFPTSGSDGPFSWQEEHFIKEAIIDAILENPVDGKLKFLHGLIEELQTNRDRMK 660

Query: 661 LKGSKETSSSKLKEDDFDDQWESWGDEDADINTNNEEVYDDMQLKLELRDRVDSLFKLLH 720
            KG+KE  SS++K+DDFDDQWESWGD+DADINT NEEVYDDMQLKLELRDRVDSLFK LH
Sbjct: 661 SKGTKEMGSSQIKDDDFDDQWESWGDDDADINTTNEEVYDDMQLKLELRDRVDSLFKTLH 720

Query: 721 KLSSSKTRNLLLKETLNSENMLHGDQYANKGVLYKLLARILNKHDLPSLEYHSSTMGRLF 780
           KLS +K  NLLLKETLNSEN+L+GDQYANKGVLYKLL RILNKHDLP+LEYHSSTMGRLF
Sbjct: 721 KLSGTKKINLLLKETLNSENILNGDQYANKGVLYKLLTRILNKHDLPNLEYHSSTMGRLF 780

Query: 781 KSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSENGRPDIELIIGGTTFL 840
           KSGFGRFGLGQAKPSLADQNVILVFV+GGINGLEVREAQEALSE+GRPDIELI+GGTTFL
Sbjct: 781 KSGFGRFGLGQAKPSLADQNVILVFVIGGINGLEVREAQEALSESGRPDIELIVGGTTFL 840

Query: 841 TPDAMFDLLLGDSAYV 857
           TP  MFDLLLGDSAYV
Sbjct: 841 TPHDMFDLLLGDSAYV 856

BLAST of MS022474 vs. TAIR 10
Match: AT2G42700.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: vesicle-mediated transport, vesicle docking involved in exocytosis; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Sec1-like protein (InterPro:IPR001619); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 960.7 bits (2482), Expect = 8.0e-280
Identity = 513/871 (58.90%), Postives = 645/871 (74.05%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MAL+DV  SCL+SI +I + ++ +I+Y+DAGC ESFQ +G FPL L+ G   VCSLEN+T
Sbjct: 1   MALIDVAISCLNSIREIEEDVKDAIVYIDAGCTESFQFVGAFPLFLELGARAVCSLENMT 60

Query: 61  SLDAVVDWNPAS--AKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAY 120
           SLDAV DWN  S  AK++V++TSRLL+DAHRY+LRCL+ H+GV  CT+FTSISE +HSA 
Sbjct: 61  SLDAVADWNSKSDCAKRIVIMTSRLLNDAHRYMLRCLSTHEGVQRCTVFTSISEGSHSAI 120

Query: 121 PDSPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEED 180
           PDSPLGPDAY EYE+LL+QDY E  KK +K    S+D+ + K+ S+ +     LT    +
Sbjct: 121 PDSPLGPDAYREYETLLVQDYNEHTKKSDK---ISKDKGVSKFSSALES----LTMEPIE 180

Query: 181 ITHLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEAC 240
             +++ SS G                Q LVVSVHHFP+I+CPF+PR FVLPS+G +AEA 
Sbjct: 181 SENVDISSGG---------------AQGLVVSVHHFPLIICPFTPRAFVLPSQGSVAEAS 240

Query: 241 LSAESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKT 300
           LS + E+SLS GLPP+ TG   D DDVPPGATLTAHF+Y  A KM+LK+EIFS+GD SK 
Sbjct: 241 LSRQHEDSLSFGLPPISTGSMSDTDDVPPGATLTAHFLYQLALKMELKLEIFSLGDQSKN 300

Query: 301 VGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFT 360
           VGKILTDMSS+YDV RRK+SAGLLLVDRTLDL+TPCCHGDSL DR+F SLPR +R +S  
Sbjct: 301 VGKILTDMSSVYDVARRKRSAGLLLVDRTLDLITPCCHGDSLFDRIFSSLPRAERFSS-- 360

Query: 361 HVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNST 420
                +  LK G P   R  LDV++PL E+L E+ SK     L E IE FL GW+S  S 
Sbjct: 361 -----QAQLKQGVPSINRPSLDVQVPLGELLNEEPSKIRDSGLPEGIEAFLRGWDSYTSA 420

Query: 421 SQNIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKW 480
            QN+    E ++    +S     ELL+G  V++E FRGTPYLEA++DRKTKDG+VL+KKW
Sbjct: 421 PQNVGLFNECDK----KSTTNWTELLNGSLVATECFRGTPYLEAMIDRKTKDGSVLVKKW 480

Query: 481 LQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEEL 540
           LQE +R+E++ VN + RPG  +K EL+++IKAL++SQS LLKN+GI+QL AA   AL+E 
Sbjct: 481 LQEALRRENISVNVRARPGYATKPELQAMIKALSQSQSSLLKNKGIIQLGAATAAALDES 540

Query: 541 NSARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLE---------SSKGVLS 600
            SA+WD F SAE  L  SA DT+QGLAAQI DLINKS  V +L+         SS+G+LS
Sbjct: 541 QSAKWDTFSSAEMMLNVSAGDTSQGLAAQISDLINKSA-VAELQAKKNEKPDSSSRGLLS 600

Query: 601 FQDSLLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLI 660
           F+D+LLLTI GYILAG+NFPTSG  GPFSWQEEHF+KEAI+DA+LENP  G LKFL+GL 
Sbjct: 601 FRDALLLTIVGYILAGENFPTSGSGGPFSWQEEHFLKEAIVDAVLENPSAGNLKFLNGLT 660

Query: 661 EELETNRERIRLKGSKETSSSKLKEDDF--DDQWESWGDEDAD--INTNNEEVYDDMQLK 720
           EELE    R++ + +KE  S    + D   DD W  WGDE+ +   N+  +E YDDMQLK
Sbjct: 661 EELEGRLNRLKSEETKEIPSDDQLDIDALDDDPWGKWGDEEEEEVDNSKADESYDDMQLK 720

Query: 721 LELRDRVDSLFKLLHKLSSSKTRNLLLKE-TLNSENMLHGDQYANKGVLYKLLARILNKH 780
           L+LRDRVDSLF+ LHKLSS +TRNL L+E +L SE+   G+   NKG++Y+L+ ++L+K 
Sbjct: 721 LDLRDRVDSLFRFLHKLSSLRTRNLPLREGSLASESSFPGEPSGNKGLVYRLITKVLSKQ 780

Query: 781 DLPSLEYHSSTMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVREAQEALSE 840
           ++P LEYHSST+GR  KSGFGRFGLGQAKPSLADQ+VILVFV+GGING+EV EAQEA+SE
Sbjct: 781 EIPGLEYHSSTVGRFIKSGFGRFGLGQAKPSLADQSVILVFVIGGINGIEVLEAQEAVSE 837

Query: 841 NGRPDIELIIGGTTFLTPDAMFDLLLGDSAY 856
           +GRPDI L+IGGTT LTPD MF+LLLG  ++
Sbjct: 841 SGRPDINLVIGGTTLLTPDDMFELLLGQFSH 837

BLAST of MS022474 vs. TAIR 10
Match: AT2G42700.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: vesicle-mediated transport, vesicle docking involved in exocytosis; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Sec1-like protein (InterPro:IPR001619). )

HSP 1 Score: 945.7 bits (2443), Expect = 2.7e-275
Identity = 513/900 (57.00%), Postives = 645/900 (71.67%), Query Frame = 0

Query: 1   MALLDVTRSCLDSINQISDHIEGSILYLDAGCVESFQTLGGFPLLLDHGVHVVCSLENLT 60
           MAL+DV  SCL+SI +I + ++ +I+Y+DAGC ESFQ +G FPL L+ G   VCSLEN+T
Sbjct: 1   MALIDVAISCLNSIREIEEDVKDAIVYIDAGCTESFQFVGAFPLFLELGARAVCSLENMT 60

Query: 61  SLDAVVDWNPAS--AKKLVVITSRLLSDAHRYILRCLTAHQGVHHCTIFTSISEIAHSAY 120
           SLDAV DWN  S  AK++V++TSRLL+DAHRY+LRCL+ H+GV  CT+FTSISE +HSA 
Sbjct: 61  SLDAVADWNSKSDCAKRIVIMTSRLLNDAHRYMLRCLSTHEGVQRCTVFTSISEGSHSAI 120

Query: 121 PDSPLGPDAYHEYESLLIQDYEELVKKGEKKAVQSEDRNLEKYISSEDEGWSRLTSSEED 180
           PDSPLGPDAY EYE+LL+QDY E  KK +K    S+D+ + K+ S+ +     LT    +
Sbjct: 121 PDSPLGPDAYREYETLLVQDYNEHTKKSDK---ISKDKGVSKFSSALES----LTMEPIE 180

Query: 181 ITHLEASSSGRDSYEDILTSHREDVGQKLVVSVHHFPMILCPFSPRVFVLPSEGLIAEAC 240
             +++ SS G                Q LVVSVHHFP+I+CPF+PR FVLPS+G +AEA 
Sbjct: 181 SENVDISSGG---------------AQGLVVSVHHFPLIICPFTPRAFVLPSQGSVAEAS 240

Query: 241 LSAESENSLSPGLPPLYTGLPPDGDDVPPGATLTAHFIYHFAAKMDLKMEIFSIGDLSKT 300
           LS + E+SLS GLPP+ TG   D DDVPPGATLTAHF+Y  A KM+LK+EIFS+GD SK 
Sbjct: 241 LSRQHEDSLSFGLPPISTGSMSDTDDVPPGATLTAHFLYQLALKMELKLEIFSLGDQSKN 300

Query: 301 VGKILTDMSSLYDVGRRKKSAGLLLVDRTLDLLTPCCHGDSLVDRMFLSLPRRKRMASFT 360
           VGKILTDMSS+YDV RRK+SAGLLLVDRTLDL+TPCCHGDSL DR+F SLPR +R +S  
Sbjct: 301 VGKILTDMSSVYDVARRKRSAGLLLVDRTLDLITPCCHGDSLFDRIFSSLPRAERFSS-- 360

Query: 361 HVKGPETSLKHGPPICRRAPLDVRIPLAEILTEDGSKADKFRLGERIEVFLSGWNSGNST 420
                +  LK G P   R  LDV++PL E+L E+ SK     L E IE FL GW+S  S 
Sbjct: 361 -----QAQLKQGVPSINRPSLDVQVPLGELLNEEPSKIRDSGLPEGIEAFLRGWDSYTSA 420

Query: 421 SQNIDKSGESNRDQNGQSLFYDPELLSGCFVSSENFRGTPYLEAILDRKTKDGAVLIKKW 480
            QN+    E ++    +S     ELL+G  V++E FRGTPYLEA++DRKTKDG+VL+KKW
Sbjct: 421 PQNVGLFNECDK----KSTTNWTELLNGSLVATECFRGTPYLEAMIDRKTKDGSVLVKKW 480

Query: 481 LQETMRKESVVVNGKIRPGVPSKLELESIIKALAKSQSCLLKNRGILQLAAAATVALEEL 540
           LQE +R+E++ VN + RPG  +K EL+++IKAL++SQS LLKN+GI+QL AA   AL+E 
Sbjct: 481 LQEALRRENISVNVRARPGYATKPELQAMIKALSQSQSSLLKNKGIIQLGAATAAALDES 540

Query: 541 NSARWDAFLSAEKTLRASAEDTTQGLAAQIVDLINKSVLVGKLE---------SSKGVLS 600
            SA+WD F SAE  L  SA DT+QGLAAQI DLINKS  V +L+         SS+G+LS
Sbjct: 541 QSAKWDTFSSAEMMLNVSAGDTSQGLAAQISDLINKSA-VAELQAKKNEKPDSSSRGLLS 600

Query: 601 FQDSLLLTITGYILAGDNFPTSGPDGPFSWQEEHFMKEAIIDAILENPVDGRLKFLHGLI 660
           F+D+LLLTI GYILAG+NFPTSG  GPFSWQEEHF+KEAI+DA+LENP  G LKFL+GL 
Sbjct: 601 FRDALLLTIVGYILAGENFPTSGSGGPFSWQEEHFLKEAIVDAVLENPSAGNLKFLNGLT 660

Query: 661 EELETNRERIRLKGSKETSSSKLKEDDF--DDQWESWGDEDAD--INTNNEEVYDDMQLK 720
           EELE    R++ + +KE  S    + D   DD W  WGDE+ +   N+  +E YDDMQLK
Sbjct: 661 EELEGRLNRLKSEETKEIPSDDQLDIDALDDDPWGKWGDEEEEEVDNSKADESYDDMQLK 720

Query: 721 LELRDRVDSLFKLLHKLSSSKTRNLLLKE-TLNSENMLHGDQYANKGVLYKLLARILNKH 780
           L+LRDRVDSLF+ LHKLSS +TRNL L+E +L SE+   G+   NKG++Y+L+ ++L+K 
Sbjct: 721 LDLRDRVDSLFRFLHKLSSLRTRNLPLREGSLASESSFPGEPSGNKGLVYRLITKVLSKQ 780

Query: 781 DLPSLEYHSSTMGRLFKSGFGRFGLGQAKPSLADQNVILVFVLGGINGLEVR-------- 840
           ++P LEYHSST+GR  KSGFGRFGLGQAKPSLADQ+VILVFV+GGING+EV         
Sbjct: 781 EIPGLEYHSSTVGRFIKSGFGRFGLGQAKPSLADQSVILVFVIGGINGIEVSFFITKVTS 840

Query: 841 ---------------------EAQEALSENGRPDIELIIGGTTFLTPDAMFDLLLGDSAY 856
                                EAQEA+SE+GRPDI L+IGGTT LTPD MF+LLLG  ++
Sbjct: 841 HSRCRAIFFITVYVSSIVKVLEAQEAVSESGRPDINLVIGGTTLLTPDDMFELLLGQFSH 866

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147932.10.0e+0098.49sec1 family domain-containing protein MIP3 [Momordica charantia] >XP_022147933.1... [more]
XP_038888123.10.0e+0089.72sec1 family domain-containing protein MIP3 [Benincasa hispida] >XP_038888124.1 s... [more]
KAA0036925.10.0e+0088.55sec1 family domain-containing protein MIP3 [Cucumis melo var. makuwa] >TYK05547.... [more]
XP_022952322.10.0e+0088.90sec1 family domain-containing protein MIP3 [Cucurbita moschata] >XP_022952323.1 ... [more]
XP_022968971.10.0e+0088.79sec1 family domain-containing protein MIP3 [Cucurbita maxima] >XP_022968972.1 se... [more]
Match NameE-valueIdentityDescription
F4IP691.1e-27858.90Sec1 family domain-containing protein MIP3 OS=Arabidopsis thaliana OX=3702 GN=MI... [more]
Q8BTY81.1e-1022.33Sec1 family domain-containing protein 2 OS=Mus musculus OX=10090 GN=Scfd2 PE=1 S... [more]
Q8WU763.4e-0923.23Sec1 family domain-containing protein 2 OS=Homo sapiens OX=9606 GN=SCFD2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1D2N50.0e+0098.49sec1 family domain-containing protein MIP3 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A5A7T6090.0e+0088.55Sec1 family domain-containing protein MIP3 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A6J1GK800.0e+0088.90sec1 family domain-containing protein MIP3 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HV020.0e+0088.79sec1 family domain-containing protein MIP3 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0LYK30.0e+0088.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G538250 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G42700.18.0e-28058.90FUNCTIONS IN: molecular_function unknown; INVOLVED IN: vesicle-mediated transpor... [more]
AT2G42700.22.7e-27557.00FUNCTIONS IN: molecular_function unknown; INVOLVED IN: vesicle-mediated transpor... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 642..662
NoneNo IPR availableCOILSCoilCoilcoord: 136..156
NoneNo IPR availablePANTHERPTHR11679:SF71SEC1 FAMILY DOMAIN-CONTAINING PROTEIN MIP3coord: 3..855
IPR027482Sec1-like, domain 2GENE3D3.40.50.1910coord: 684..850
e-value: 1.1E-6
score: 30.3
IPR001619Sec1-like proteinPFAMPF00995Sec1coord: 673..846
e-value: 1.5E-7
score: 31.1
IPR001619Sec1-like proteinPANTHERPTHR11679VESICLE PROTEIN SORTING-ASSOCIATEDcoord: 3..855
IPR036045Sec1-like superfamilySUPERFAMILY56815Sec1/munc18-like (SM) proteinscoord: 183..337
IPR036045Sec1-like superfamilySUPERFAMILY56815Sec1/munc18-like (SM) proteinscoord: 732..850

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022474.1MS022474.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016192 vesicle-mediated transport