MS022465 (gene) Bitter gourd (TR) v1

Overview
NameMS022465
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionWRKY protein
Locationscaffold47: 3605480 .. 3607449 (-)
RNA-Seq ExpressionMS022465
SyntenyMS022465
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCGCCGTGTTCGTACAAACCCATTTGTCTCCGAGCAAGAAGATCCCGAACCCACGTCGGACGATGGCCTCCCGCTGGAGTCACCCTCCGACGGCAATGACTCCAAGCTCGCCGCCGGCCCCGCCCCCAAAAAGAGGTCAGTTTAATTTCATCACTTTTTATTTTTTCTTGATTTATAATTGAACTGTTTGGATGTGTAATTCAGTTTTTTAACGGTGTGGATTTTGTATGATGTGCAGTAGAAGGGGCGTGCAGAAGCGAGTGGTGTCAGTACCGATCGGCGACGTGGACGGTCCTAAGAGCAAAGGGGAAGCATATCCACCGTCGGATTCGTGGGCGTGGAGGAAATACGGCCAAAAGCCCATCAAAGGTTCTCCTTATCCCAGGTACTAAATAATTGGGCATTTTTCGCAATTCTTTCTTGTTTTTTGGAAGCTTGATTTCCATTTATTTCCACTAATACAATATAATTGAGCTATAATTGATCCGAAAATTGCATTTATTTTGATGCATTTAGATAATAATTTGGGCTAATTATAGTCGAGTAATTGAAGAAACGCTTGAGATTTCGACTTTTCTTCGTGTTGTTACCGCCGGCCGCCGCCGGCCGCCTGTCTCTAACCAATAAAATATCTAAACCGCCGGCTGCTATAGGATCTGGATTGAGATTGGGCGCAATTTAGCACAAAGGCGGCTTTTGCTACTAAGCGCTGGTTTTTATTTTCCGTAGTTGGGAATCATACTCATTATCCAATCGCTCTCGCAAAGCGTGTACTGCACGCGCTCTTTTTGAATTTTGATTTTTTTTTCCTCTTCTTTTACCTAATTATCGCCGTATTTGCGCCGAATTCCTGTTTTGAATTTTGATGAACATGGTTTCTCAAAACTTGTTGTTTTTAAATATTTTTAAACAGTCTTCAAATTTCAATTTATTTTTGAACAGACGTAAATTACGAAATTATTTGAAAGATGCCTTTCACATCGAGGGCATACCGGTGAGTGGCGGCCAAACGATAGGACTTGAAATAAAAAAGTATTATTTATTGGTTTTGGCCGGCATTTATAAATCAAATTGCCTAATTAATAATTATTTTTTCAGTTTTTAATAGAAAATTAATTAAACGAATTGAAAATAATTTTTAAATTATTGATGGAATATTGAATTAACGATTCTCAAGTAAAGAAATGATTGAAATTTTTAACTTTCGCATTCCTCTATGGGGACTTGTTTGGTTATTGGCTAATGGGCTTATCTTCTTTCCCGCTAACTTAACTCTTTTCTTTTGGAATATTTGTATTGAGAGCCCACGTTTTTTTAATTATTATTTATATATTATTTTATATTTATAGGGGATATTACCGATGTAGTAGCTCCAAGGGCTGCCCCGCAAGAAAGCAAGTAGAGAGAAGCCGTGTGGACCCCACCAAGCTCGTTATCACCTACGCTTTCGACCACAACCACCAACTCCCGGCCGCCAAATCTCACCACCACCACCACCACCACCATTCTTCGCCGTCTCCCGCCGCGATCACCGCCGCTTCCGCCGCCGCCGACGACTCGTCCCCTGGGAGCACTACGACGTCGTCCTCCACGTCCTCCGGCGACCACACGAACATGACCCCCACGTCGCCGTCTCCGGCGGCCAAGTTCGAGGAGGCGGCGGCAGTCTTCGCGAGTCAGCCGGAGCTGGAACTCGGCGGCGACTCGCTGCTTATTAAACCCTGCCTCGGGGATTTCGGGTGGTTGGCTGACGTGGCGTACGATAGGATCCTCGAGGGCCCGATTTGCGGGGGAGGCGACATCTTCGACGACGCCGACGTCATGGTTTTGTCCACCAGAGTGGATGACGTGGACGAGTCGTTATTCGCGGATCTTGGGGAATTACCCGAAGGCTCCGTGATTTTCGGCCGGAGAGCGGTCCACACGGATGGACCGAACCGGACGCGTGGCAGAGTTTTAAACTGC

mRNA sequence

ATGGACCGCCGTGTTCGTACAAACCCATTTGTCTCCGAGCAAGAAGATCCCGAACCCACGTCGGACGATGGCCTCCCGCTGGAGTCACCCTCCGACGGCAATGACTCCAAGCTCGCCGCCGGCCCCGCCCCCAAAAAGAGTAGAAGGGGCGTGCAGAAGCGAGTGGTGTCAGTACCGATCGGCGACGTGGACGGTCCTAAGAGCAAAGGGGAAGCATATCCACCGTCGGATTCGTGGGCGTGGAGGAAATACGGCCAAAAGCCCATCAAAGGTTCTCCTTATCCCAGGGGATATTACCGATGTAGTAGCTCCAAGGGCTGCCCCGCAAGAAAGCAAGTAGAGAGAAGCCGTGTGGACCCCACCAAGCTCGTTATCACCTACGCTTTCGACCACAACCACCAACTCCCGGCCGCCAAATCTCACCACCACCACCACCACCACCATTCTTCGCCGTCTCCCGCCGCGATCACCGCCGCTTCCGCCGCCGCCGACGACTCGTCCCCTGGGAGCACTACGACGTCGTCCTCCACGTCCTCCGGCGACCACACGAACATGACCCCCACGTCGCCGTCTCCGGCGGCCAAGTTCGAGGAGGCGGCGGCAGTCTTCGCGAGTCAGCCGGAGCTGGAACTCGGCGGCGACTCGCTGCTTATTAAACCCTGCCTCGGGGATTTCGGGTGGTTGGCTGACGTGGCGTACGATAGGATCCTCGAGGGCCCGATTTGCGGGGGAGGCGACATCTTCGACGACGCCGACGTCATGGTTTTGTCCACCAGAGTGGATGACGTGGACGAGTCGTTATTCGCGGATCTTGGGGAATTACCCGAAGGCTCCGTGATTTTCGGCCGGAGAGCGGTCCACACGGATGGACCGAACCGGACGCGTGGCAGAGTTTTAAACTGC

Coding sequence (CDS)

ATGGACCGCCGTGTTCGTACAAACCCATTTGTCTCCGAGCAAGAAGATCCCGAACCCACGTCGGACGATGGCCTCCCGCTGGAGTCACCCTCCGACGGCAATGACTCCAAGCTCGCCGCCGGCCCCGCCCCCAAAAAGAGTAGAAGGGGCGTGCAGAAGCGAGTGGTGTCAGTACCGATCGGCGACGTGGACGGTCCTAAGAGCAAAGGGGAAGCATATCCACCGTCGGATTCGTGGGCGTGGAGGAAATACGGCCAAAAGCCCATCAAAGGTTCTCCTTATCCCAGGGGATATTACCGATGTAGTAGCTCCAAGGGCTGCCCCGCAAGAAAGCAAGTAGAGAGAAGCCGTGTGGACCCCACCAAGCTCGTTATCACCTACGCTTTCGACCACAACCACCAACTCCCGGCCGCCAAATCTCACCACCACCACCACCACCACCATTCTTCGCCGTCTCCCGCCGCGATCACCGCCGCTTCCGCCGCCGCCGACGACTCGTCCCCTGGGAGCACTACGACGTCGTCCTCCACGTCCTCCGGCGACCACACGAACATGACCCCCACGTCGCCGTCTCCGGCGGCCAAGTTCGAGGAGGCGGCGGCAGTCTTCGCGAGTCAGCCGGAGCTGGAACTCGGCGGCGACTCGCTGCTTATTAAACCCTGCCTCGGGGATTTCGGGTGGTTGGCTGACGTGGCGTACGATAGGATCCTCGAGGGCCCGATTTGCGGGGGAGGCGACATCTTCGACGACGCCGACGTCATGGTTTTGTCCACCAGAGTGGATGACGTGGACGAGTCGTTATTCGCGGATCTTGGGGAATTACCCGAAGGCTCCGTGATTTTCGGCCGGAGAGCGGTCCACACGGATGGACCGAACCGGACGCGTGGCAGAGTTTTAAACTGC

Protein sequence

MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPIGDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLNC
Homology
BLAST of MS022465 vs. NCBI nr
Match: XP_022147889.1 (probable WRKY transcription factor 69 isoform X1 [Momordica charantia])

HSP 1 Score: 596.7 bits (1537), Expect = 1.1e-166
Identity = 301/301 (100.00%), Postives = 301/301 (100.00%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
           GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG
Sbjct: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP
Sbjct: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN 300
           ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN
Sbjct: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN 300

Query: 301 C 302
           C
Sbjct: 301 C 301

BLAST of MS022465 vs. NCBI nr
Match: XP_022147890.1 (probable WRKY transcription factor 69 isoform X2 [Momordica charantia])

HSP 1 Score: 590.5 bits (1521), Expect = 8.2e-165
Identity = 300/301 (99.67%), Postives = 300/301 (99.67%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKK RRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKK-RRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
           GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG
Sbjct: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP
Sbjct: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN 300
           ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN
Sbjct: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN 300

Query: 301 C 302
           C
Sbjct: 301 C 300

BLAST of MS022465 vs. NCBI nr
Match: AQX45437.1 (WRKY transcription factor 1-like protein [Luffa aegyptiaca])

HSP 1 Score: 515.0 bits (1325), Expect = 4.4e-142
Identity = 270/302 (89.40%), Postives = 281/302 (93.05%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPF+SEQEDPE TSDDGLP ESPSDGNDSK AAGP PKKSRRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFLSEQEDPEATSDDGLP-ESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
           GDV+G KSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  GDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           TKLVITYAFDHNHQLPA KS HHHHHH+SSPS A+I AA A  DDSSPGSTTTSSSTSSG
Sbjct: 121 TKLVITYAFDHNHQLPATKS-HHHHHHNSSPSSASIAAAVAVPDDSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           D+TN TPTSP+ AAKFEEAAAVFASQPELELGGDSL+IKPC+GDFGW ADVAYDRILEGP
Sbjct: 181 DNTNATPTSPT-AAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWFADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFG-RRAVHTDGPNRTRGRVL 300
           ICGGGDIFDDADVMVLSTR DD +ESLFADLGELPEGSV+FG RRAV  DGPNRT GRVL
Sbjct: 241 ICGGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRAVEPDGPNRTCGRVL 299

Query: 301 NC 302
           +C
Sbjct: 301 DC 299

BLAST of MS022465 vs. NCBI nr
Match: XP_038888987.1 (probable WRKY transcription factor 69 isoform X1 [Benincasa hispida])

HSP 1 Score: 511.9 bits (1317), Expect = 3.7e-141
Identity = 267/302 (88.41%), Postives = 278/302 (92.05%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPFVSEQEDPEPTSDDGLP ESPSDGNDSK AAGP PKKSRRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFVSEQEDPEPTSDDGLP-ESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
            DV+G KSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  SDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           +KLVITYAFDHNHQLP  KS  HHHHH+SSPS A I AASAAAD SSPGSTTTSSSTSSG
Sbjct: 121 SKLVITYAFDHNHQLPVTKS--HHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           D+TN  P+SP+ AAKFEEAAAVFASQPELELGGDSL+IKPC+GDFGWLADVAYDRILEGP
Sbjct: 181 DNTNAAPSSPAAAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFG-RRAVHTDGPNRTRGRVL 300
           ICGGGDIFDDADVMVLSTR DD +ESLFADLGELPEGSV+FG RR V  DGPNRT G VL
Sbjct: 241 ICGGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTCGTVL 299

Query: 301 NC 302
           +C
Sbjct: 301 DC 299

BLAST of MS022465 vs. NCBI nr
Match: XP_038888988.1 (probable WRKY transcription factor 69 isoform X2 [Benincasa hispida])

HSP 1 Score: 505.8 bits (1301), Expect = 2.7e-139
Identity = 266/302 (88.08%), Postives = 277/302 (91.72%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPFVSEQEDPEPTSDDGLP ESPSDGNDSK AAGP PKK RRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFVSEQEDPEPTSDDGLP-ESPSDGNDSKPAAGPPPKK-RRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
            DV+G KSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  SDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           +KLVITYAFDHNHQLP  KS  HHHHH+SSPS A I AASAAAD SSPGSTTTSSSTSSG
Sbjct: 121 SKLVITYAFDHNHQLPVTKS--HHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           D+TN  P+SP+ AAKFEEAAAVFASQPELELGGDSL+IKPC+GDFGWLADVAYDRILEGP
Sbjct: 181 DNTNAAPSSPAAAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFG-RRAVHTDGPNRTRGRVL 300
           ICGGGDIFDDADVMVLSTR DD +ESLFADLGELPEGSV+FG RR V  DGPNRT G VL
Sbjct: 241 ICGGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTCGTVL 298

Query: 301 NC 302
           +C
Sbjct: 301 DC 298

BLAST of MS022465 vs. ExPASy Swiss-Prot
Match: Q93WV5 (Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.5e-47
Identity = 125/286 (43.71%), Postives = 161/286 (56.29%), Query Frame = 0

Query: 11  VSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPIGDVDGPKSKG 70
           + E +D E  + + +  ESPS   DSK++  P PKK RR V+KRVVSVPI DV+G KS+G
Sbjct: 7   IQESDDEEDETYNDVVPESPSSCEDSKISK-PTPKK-RRNVEKRVVSVPIADVEGSKSRG 66

Query: 71  EAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFD 130
           E YPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP+KL+ITYA D
Sbjct: 67  EVYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACD 126

Query: 131 HNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSP 190
           HNH  P++ ++   HH  S      +   +A  ++               +   +T    
Sbjct: 127 HNHPFPSSSANTKSHHRSS------VVLKTAKKEE---------EYEEEEEELTVTAAEE 186

Query: 191 SPAAKFEEAAAVFASQPELELGGDSLLIKPC---LGDFGWLADVAYDRILEGPICGGGDI 250
            PA              +L      LL+  C   +G+FGW  D +           G   
Sbjct: 187 PPAGL------------DLSHVDSPLLLGGCYSEIGEFGWFYDASISS------SSGSSN 246

Query: 251 FDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNR 294
           F D  +    +   + DESLF DLG+LP+ + +F R  V T+  +R
Sbjct: 247 FLDVTLERGFSVGQEEDESLFGDLGDLPDCASVFRRGTVATEEQHR 257

BLAST of MS022465 vs. ExPASy Swiss-Prot
Match: Q9LP56 (Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.5e-41
Identity = 113/275 (41.09%), Postives = 145/275 (52.73%), Query Frame = 0

Query: 16  DPEPTSDDGLPLESPSDGNDSK-LAAGPAPKKSRRGVQKRVVSVPIGDVDGPKSKGEAYP 75
           D E + + G    + S  N  K L +  +PK+SRR V+KRVV+VP+ +++G + KG+  P
Sbjct: 13  DHESSQETGPESPNSSTFNGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTP 72

Query: 76  PSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQ 135
           PSDSWAWRKYGQKPIKGSPYPRGYYRCSS+KGCPARKQVERSR DPT ++ITY  +HNH 
Sbjct: 73  PSDSWAWRKYGQKPIKGSPYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHP 132

Query: 136 LPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAA 195
            P                                     +SST +G      P  P P  
Sbjct: 133 WP------------------------------------LTSSTRNGPKPKPEP-KPEPEP 192

Query: 196 KFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADV--AYDRILEGPICGG--GDIFDD 255
           + E  A        + LG        C+ +F W  ++      ILE PI           
Sbjct: 193 EVEPEAEE-EDNKFMVLGRGIETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAVSG 249

Query: 256 ADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRA 286
           AD + +   + + DESLFADLGELPE SV+F  R+
Sbjct: 253 ADDVAVFFPMGEEDESLFADLGELPECSVVFRHRS 249

BLAST of MS022465 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 4.1e-26
Identity = 74/166 (44.58%), Postives = 97/166 (58.43%), Query Frame = 0

Query: 46  KSRRGVQKRVVSVPIGDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK 105
           K R+   K+VV +P       +S GE   PSD WAWRKYGQKPIKGSP+PRGYYRCSSSK
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 106 GCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADD 165
           GC ARKQVERSR DP  LVITY  +HNH  P  +       +  + S  + T++S+  + 
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQR-------NALAGSTRSSTSSSSNPNP 307

Query: 166 SSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELEL 212
           S P +   +SS+    +T   P+S +P      +A       ++EL
Sbjct: 308 SKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMEL 345

BLAST of MS022465 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.9e-25
Identity = 77/156 (49.36%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 46  KSRRGVQKRVVSVPIGDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK 105
           K R+   K+VV +P       +S GE   PSD WAWRKYGQKPIKGSPYPRGYYRCSSSK
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 106 GCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPA-AITAASAAAD 165
           GC ARKQVERSR DP  LVITY  +HNH  P  ++        SS S     + +S AA 
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLNPSSKSSTAAA 305

Query: 166 DSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAA 201
            +SP S    +++S  +  N    S S    F+ AA
Sbjct: 306 TTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAA 340

BLAST of MS022465 vs. ExPASy Swiss-Prot
Match: Q9SUS1 (Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.3e-19
Identity = 80/205 (39.02%), Postives = 102/205 (49.76%), Query Frame = 0

Query: 11  VSEQEDPEPTSDD------GLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPIGDVD 70
           VS  ED +P  DD      G  L + S  +  +++   + KKS++  QKRVV        
Sbjct: 74  VSLPEDSKPFRDDKKQRSHGCLLSNGSRADHIRISESKS-KKSKKNQQKRVV-------- 133

Query: 71  GPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTKLV 130
             +   E    SD+WAWRKYGQKPIKGSPYPR YYRCSSSKGC ARKQVER+  +P K  
Sbjct: 134 --EQVKEENLLSDAWAWRKYGQKPIKGSPYPRSYYRCSSSKGCLARKQVERNPQNPEKFT 193

Query: 131 ITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSS----TSSG 190
           ITY  +HNH+LP  ++                 A S  A  S P  T T  S     SS 
Sbjct: 194 ITYTNEHNHELPTRRNS---------------LAGSTRAKTSQPKPTLTKKSEKEVVSSP 252

Query: 191 DHTNMTPTSPSPAAKFEEAAAVFAS 206
               M P++   +   +E +    S
Sbjct: 254 TSNPMIPSADESSVAVQEMSVAETS 252

BLAST of MS022465 vs. ExPASy TrEMBL
Match: A0A6J1D2C7 (probable WRKY transcription factor 69 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016715 PE=4 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 5.5e-167
Identity = 301/301 (100.00%), Postives = 301/301 (100.00%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
           GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG
Sbjct: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP
Sbjct: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN 300
           ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN
Sbjct: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN 300

Query: 301 C 302
           C
Sbjct: 301 C 301

BLAST of MS022465 vs. ExPASy TrEMBL
Match: A0A6J1D1E0 (probable WRKY transcription factor 69 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016715 PE=4 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.0e-165
Identity = 300/301 (99.67%), Postives = 300/301 (99.67%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKK RRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKK-RRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
           GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG
Sbjct: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP
Sbjct: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN 300
           ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN
Sbjct: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLN 300

Query: 301 C 302
           C
Sbjct: 301 C 300

BLAST of MS022465 vs. ExPASy TrEMBL
Match: A0A1S6YJF1 (WRKY transcription factor 1-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.1e-142
Identity = 270/302 (89.40%), Postives = 281/302 (93.05%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPF+SEQEDPE TSDDGLP ESPSDGNDSK AAGP PKKSRRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFLSEQEDPEATSDDGLP-ESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
           GDV+G KSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  GDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           TKLVITYAFDHNHQLPA KS HHHHHH+SSPS A+I AA A  DDSSPGSTTTSSSTSSG
Sbjct: 121 TKLVITYAFDHNHQLPATKS-HHHHHHNSSPSSASIAAAVAVPDDSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           D+TN TPTSP+ AAKFEEAAAVFASQPELELGGDSL+IKPC+GDFGW ADVAYDRILEGP
Sbjct: 181 DNTNATPTSPT-AAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWFADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFG-RRAVHTDGPNRTRGRVL 300
           ICGGGDIFDDADVMVLSTR DD +ESLFADLGELPEGSV+FG RRAV  DGPNRT GRVL
Sbjct: 241 ICGGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRAVEPDGPNRTCGRVL 299

Query: 301 NC 302
           +C
Sbjct: 301 DC 299

BLAST of MS022465 vs. ExPASy TrEMBL
Match: A0A6J1GKJ3 (probable WRKY transcription factor 69 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454826 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.3e-139
Identity = 268/302 (88.74%), Postives = 277/302 (91.72%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPFVSEQEDPE TSDDGLP ESPSD NDSK AAGP PKKSRRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFVSEQEDPEATSDDGLP-ESPSDDNDSKPAAGPPPKKSRRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
           GDV+G KSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  GDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           TKLVITYAFDHNHQLPA KS  HHHHH+SSPS AAI   +AA D SSPGSTTTSSSTSSG
Sbjct: 121 TKLVITYAFDHNHQLPATKS--HHHHHNSSPSSAAI---AAAPDSSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           DHTN  P S S AAKFEEAAAVFASQPELELGGDSL+IKPC+GDFGWLADVAYDRILEGP
Sbjct: 181 DHTNTDP-SLSAAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFG-RRAVHTDGPNRTRGRVL 300
           ICGGGDIFDDADVMVLSTR +D DESLFADLGELPEGS++FG RRAV  DGPNRTRGRVL
Sbjct: 241 ICGGGDIFDDADVMVLSTRGNDEDESLFADLGELPEGSMVFGRRRAVQPDGPNRTRGRVL 295

Query: 301 NC 302
           +C
Sbjct: 301 DC 295

BLAST of MS022465 vs. ExPASy TrEMBL
Match: A0A6J1I0A2 (probable WRKY transcription factor 69 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468262 PE=4 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.1e-138
Identity = 266/302 (88.08%), Postives = 277/302 (91.72%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPI 60
           MDRRVRTNPFVSEQEDPE TSDDGLP ESPSD NDSK AAGP PKKSRRGVQKRVVSVPI
Sbjct: 1   MDRRVRTNPFVSEQEDPEATSDDGLP-ESPSDDNDSKPAAGPPPKKSRRGVQKRVVSVPI 60

Query: 61  GDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120
           GDV+G KSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP
Sbjct: 61  GDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP 120

Query: 121 TKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSG 180
           TKLVITYAFDHNHQLPA KS  HHHHH+SSPS AAI   +AA D SSPGSTTTSSSTSSG
Sbjct: 121 TKLVITYAFDHNHQLPATKS--HHHHHNSSPSSAAI---AAAPDSSSPGSTTTSSSTSSG 180

Query: 181 DHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGP 240
           DHTN  P+ P+ AAKFEEAAAVFASQPELELGGDSL+IKPC+GDFGWLADVAYDRILEGP
Sbjct: 181 DHTNTDPSFPA-AAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGP 240

Query: 241 ICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFG-RRAVHTDGPNRTRGRVL 300
           ICGGGDIFDDADVMVLSTR DD DESLFADLGELPEGS++FG RRAV  DGPNRT GR+L
Sbjct: 241 ICGGGDIFDDADVMVLSTRGDDEDESLFADLGELPEGSMVFGRRRAVQLDGPNRTCGRIL 295

Query: 301 NC 302
           +C
Sbjct: 301 DC 295

BLAST of MS022465 vs. TAIR 10
Match: AT3G58710.1 (WRKY DNA-binding protein 69 )

HSP 1 Score: 196.8 bits (499), Expect = 2.5e-50
Identity = 126/286 (44.06%), Postives = 162/286 (56.64%), Query Frame = 0

Query: 11  VSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPIGDVDGPKSKG 70
           + E +D E  + + +  ESPS   DSK++  P PKKSRR V+KRVVSVPI DV+G KS+G
Sbjct: 7   IQESDDEEDETYNDVVPESPSSCEDSKISK-PTPKKSRRNVEKRVVSVPIADVEGSKSRG 66

Query: 71  EAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFD 130
           E YPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP+KL+ITYA D
Sbjct: 67  EVYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACD 126

Query: 131 HNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSP 190
           HNH  P++ ++   HH  S      +   +A  ++               +   +T    
Sbjct: 127 HNHPFPSSSANTKSHHRSS------VVLKTAKKEE---------EYEEEEEELTVTAAEE 186

Query: 191 SPAAKFEEAAAVFASQPELELGGDSLLIKPC---LGDFGWLADVAYDRILEGPICGGGDI 250
            PA              +L      LL+  C   +G+FGW  D +           G   
Sbjct: 187 PPAGL------------DLSHVDSPLLLGGCYSEIGEFGWFYDASISS------SSGSSN 246

Query: 251 FDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNR 294
           F D  +    +   + DESLF DLG+LP+ + +F R  V T+  +R
Sbjct: 247 FLDVTLERGFSVGQEEDESLFGDLGDLPDCASVFRRGTVATEEQHR 258

BLAST of MS022465 vs. TAIR 10
Match: AT3G58710.2 (WRKY DNA-binding protein 69 )

HSP 1 Score: 190.7 bits (483), Expect = 1.8e-48
Identity = 125/286 (43.71%), Postives = 161/286 (56.29%), Query Frame = 0

Query: 11  VSEQEDPEPTSDDGLPLESPSDGNDSKLAAGPAPKKSRRGVQKRVVSVPIGDVDGPKSKG 70
           + E +D E  + + +  ESPS   DSK++  P PKK RR V+KRVVSVPI DV+G KS+G
Sbjct: 7   IQESDDEEDETYNDVVPESPSSCEDSKISK-PTPKK-RRNVEKRVVSVPIADVEGSKSRG 66

Query: 71  EAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFD 130
           E YPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP+KL+ITYA D
Sbjct: 67  EVYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACD 126

Query: 131 HNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSP 190
           HNH  P++ ++   HH  S      +   +A  ++               +   +T    
Sbjct: 127 HNHPFPSSSANTKSHHRSS------VVLKTAKKEE---------EYEEEEEELTVTAAEE 186

Query: 191 SPAAKFEEAAAVFASQPELELGGDSLLIKPC---LGDFGWLADVAYDRILEGPICGGGDI 250
            PA              +L      LL+  C   +G+FGW  D +           G   
Sbjct: 187 PPAGL------------DLSHVDSPLLLGGCYSEIGEFGWFYDASISS------SSGSSN 246

Query: 251 FDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNR 294
           F D  +    +   + DESLF DLG+LP+ + +F R  V T+  +R
Sbjct: 247 FLDVTLERGFSVGQEEDESLFGDLGDLPDCASVFRRGTVATEEQHR 257

BLAST of MS022465 vs. TAIR 10
Match: AT1G29280.1 (WRKY DNA-binding protein 65 )

HSP 1 Score: 169.9 bits (429), Expect = 3.2e-42
Identity = 113/275 (41.09%), Postives = 145/275 (52.73%), Query Frame = 0

Query: 16  DPEPTSDDGLPLESPSDGNDSK-LAAGPAPKKSRRGVQKRVVSVPIGDVDGPKSKGEAYP 75
           D E + + G    + S  N  K L +  +PK+SRR V+KRVV+VP+ +++G + KG+  P
Sbjct: 13  DHESSQETGPESPNSSTFNGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTP 72

Query: 76  PSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQ 135
           PSDSWAWRKYGQKPIKGSPYPRGYYRCSS+KGCPARKQVERSR DPT ++ITY  +HNH 
Sbjct: 73  PSDSWAWRKYGQKPIKGSPYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHP 132

Query: 136 LPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAA 195
            P                                     +SST +G      P  P P  
Sbjct: 133 WP------------------------------------LTSSTRNGPKPKPEP-KPEPEP 192

Query: 196 KFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADV--AYDRILEGPICGG--GDIFDD 255
           + E  A        + LG        C+ +F W  ++      ILE PI           
Sbjct: 193 EVEPEAEE-EDNKFMVLGRGIETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAVSG 249

Query: 256 ADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRA 286
           AD + +   + + DESLFADLGELPE SV+F  R+
Sbjct: 253 ADDVAVFFPMGEEDESLFADLGELPECSVVFRHRS 249

BLAST of MS022465 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 120.2 bits (300), Expect = 2.9e-27
Identity = 74/166 (44.58%), Postives = 97/166 (58.43%), Query Frame = 0

Query: 46  KSRRGVQKRVVSVPIGDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK 105
           K R+   K+VV +P       +S GE   PSD WAWRKYGQKPIKGSP+PRGYYRCSSSK
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 106 GCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADD 165
           GC ARKQVERSR DP  LVITY  +HNH  P  +       +  + S  + T++S+  + 
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQR-------NALAGSTRSSTSSSSNPNP 307

Query: 166 SSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELEL 212
           S P +   +SS+    +T   P+S +P      +A       ++EL
Sbjct: 308 SKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMEL 345

BLAST of MS022465 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 116.3 bits (290), Expect = 4.2e-26
Identity = 77/156 (49.36%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 46  KSRRGVQKRVVSVPIGDVDGPKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK 105
           K R+   K+VV +P       +S GE   PSD WAWRKYGQKPIKGSPYPRGYYRCSSSK
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 106 GCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPA-AITAASAAAD 165
           GC ARKQVERSR DP  LVITY  +HNH  P  ++        SS S     + +S AA 
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLNPSSKSSTAAA 305

Query: 166 DSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAA 201
            +SP S    +++S  +  N    S S    F+ AA
Sbjct: 306 TTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAA 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147889.11.1e-166100.00probable WRKY transcription factor 69 isoform X1 [Momordica charantia][more]
XP_022147890.18.2e-16599.67probable WRKY transcription factor 69 isoform X2 [Momordica charantia][more]
AQX45437.14.4e-14289.40WRKY transcription factor 1-like protein [Luffa aegyptiaca][more]
XP_038888987.13.7e-14188.41probable WRKY transcription factor 69 isoform X1 [Benincasa hispida][more]
XP_038888988.12.7e-13988.08probable WRKY transcription factor 69 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q93WV52.5e-4743.71Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 ... [more]
Q9LP564.5e-4141.09Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 ... [more]
Q9SA804.1e-2644.58Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ... [more]
O647475.9e-2549.36Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
Q9SUS11.3e-1939.02Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 ... [more]
Match NameE-valueIdentityDescription
A0A6J1D2C75.5e-167100.00probable WRKY transcription factor 69 isoform X1 OS=Momordica charantia OX=3673 ... [more]
A0A6J1D1E04.0e-16599.67probable WRKY transcription factor 69 isoform X2 OS=Momordica charantia OX=3673 ... [more]
A0A1S6YJF12.1e-14289.40WRKY transcription factor 1-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A6J1GKJ38.3e-13988.74probable WRKY transcription factor 69 isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1I0A21.1e-13888.08probable WRKY transcription factor 69 isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
AT3G58710.12.5e-5044.06WRKY DNA-binding protein 69 [more]
AT3G58710.21.8e-4843.71WRKY DNA-binding protein 69 [more]
AT1G29280.13.2e-4241.09WRKY DNA-binding protein 65 [more]
AT1G30650.12.9e-2744.58WRKY DNA-binding protein 14 [more]
AT2G34830.14.2e-2649.36WRKY DNA-binding protein 35 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 75..135
e-value: 2.5E-34
score: 130.1
IPR003657WRKY domainPFAMPF03106WRKYcoord: 77..134
e-value: 1.2E-24
score: 86.4
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 76..136
score: 31.19219
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 65..137
e-value: 4.3E-29
score: 102.1
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 71..136
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 163..191
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 137..192
NoneNo IPR availablePANTHERPTHR32096:SF19OS01G0750100 PROTEINcoord: 30..285
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 30..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022465.1MS022465.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding