MS022464 (gene) Bitter gourd (TR) v1

Overview


Name	MS022464
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	WRKY protein
Location	scaffold47: 3600495 .. 3601112 (-)
RNA-Seq Expression	MS022464
Synteny	MS022464

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGGGATATTACCGATGTAGTAGCTCCAAGGGCTGCCCCGCAAGAAAGCAAGTAGAGAGAAGCCGTGTGGACCCCACCAAGCTCGTTATCACCTACGCTTTCGACCACAACCACCAACTCCCGGCCGCCAAATCTCACCACCACCACCACCACCACCATTCTTCGCCGTCTCCCGCCGCGATCACCGCCGCTTCCGCCGCCGCCGACGACTCGTCCCCTGGGAGCACTACGACGTCGTCCTCCACGTCCTCCGGCGACCACACGAACATGACCCCCACGTCGCCGTCTCCGGCGGCCAAGTTCGAGGAGGCGGCGGCAGTCTTCGCGAGTCAGCCGGAGCTGGAACTCGGCGGCGACTCGCTGCTTATTAAACCCTGCCTCGGGGATTTCGGGTGGTTGGCTGACGTGGCGTACGATAGGATCCTCGAGGGCCCGATTTGCGGGGGAGGCGACATCTTCGACGACGCCGACGTCATGGTTTTGTCCACCAGAGTGGATGACGTGGACGAGTCGTTATTCGCGGATCTTGGGGAATTACCCGAAGGCTCCGTGATTTTCGGCCGGAGAGCGGTCCACACGGATGGACCGAACCGGACGCGTGGCAGAGTTTTAAACTGC

mRNA sequence

AGGGGATATTACCGATGTAGTAGCTCCAAGGGCTGCCCCGCAAGAAAGCAAGTAGAGAGAAGCCGTGTGGACCCCACCAAGCTCGTTATCACCTACGCTTTCGACCACAACCACCAACTCCCGGCCGCCAAATCTCACCACCACCACCACCACCACCATTCTTCGCCGTCTCCCGCCGCGATCACCGCCGCTTCCGCCGCCGCCGACGACTCGTCCCCTGGGAGCACTACGACGTCGTCCTCCACGTCCTCCGGCGACCACACGAACATGACCCCCACGTCGCCGTCTCCGGCGGCCAAGTTCGAGGAGGCGGCGGCAGTCTTCGCGAGTCAGCCGGAGCTGGAACTCGGCGGCGACTCGCTGCTTATTAAACCCTGCCTCGGGGATTTCGGGTGGTTGGCTGACGTGGCGTACGATAGGATCCTCGAGGGCCCGATTTGCGGGGGAGGCGACATCTTCGACGACGCCGACGTCATGGTTTTGTCCACCAGAGTGGATGACGTGGACGAGTCGTTATTCGCGGATCTTGGGGAATTACCCGAAGGCTCCGTGATTTTCGGCCGGAGAGCGGTCCACACGGATGGACCGAACCGGACGCGTGGCAGAGTTTTAAACTGC

Coding sequence (CDS)

AGGGGATATTACCGATGTAGTAGCTCCAAGGGCTGCCCCGCAAGAAAGCAAGTAGAGAGAAGCCGTGTGGACCCCACCAAGCTCGTTATCACCTACGCTTTCGACCACAACCACCAACTCCCGGCCGCCAAATCTCACCACCACCACCACCACCACCATTCTTCGCCGTCTCCCGCCGCGATCACCGCCGCTTCCGCCGCCGCCGACGACTCGTCCCCTGGGAGCACTACGACGTCGTCCTCCACGTCCTCCGGCGACCACACGAACATGACCCCCACGTCGCCGTCTCCGGCGGCCAAGTTCGAGGAGGCGGCGGCAGTCTTCGCGAGTCAGCCGGAGCTGGAACTCGGCGGCGACTCGCTGCTTATTAAACCCTGCCTCGGGGATTTCGGGTGGTTGGCTGACGTGGCGTACGATAGGATCCTCGAGGGCCCGATTTGCGGGGGAGGCGACATCTTCGACGACGCCGACGTCATGGTTTTGTCCACCAGAGTGGATGACGTGGACGAGTCGTTATTCGCGGATCTTGGGGAATTACCCGAAGGCTCCGTGATTTTCGGCCGGAGAGCGGTCCACACGGATGGACCGAACCGGACGCGTGGCAGAGTTTTAAACTGC

Protein sequence

RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAAITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDSLLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELPEGSVIFGRRAVHTDGPNRTRGRVLNC

Homology

[Close]

BLAST of MS022464 vs. NCBI nr
Match: XP_022147889.1 (probable WRKY transcription factor 69 isoform X1 [Momordica charantia])

HSP 1 Score: 406.0 bits (1042), Expect = 2.0e-109
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA
Sbjct: 96  RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 155

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS
Sbjct: 156 ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 215

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP
Sbjct: 216 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 275

Query: 181 EGSVIFGRRAVHTDGPNRTRGRVLNC 207
           EGSVIFGRRAVHTDGPNRTRGRVLNC
Sbjct: 276 EGSVIFGRRAVHTDGPNRTRGRVLNC 301

[Close]

BLAST of MS022464 vs. NCBI nr
Match: XP_022147890.1 (probable WRKY transcription factor 69 isoform X2 [Momordica charantia])

HSP 1 Score: 406.0 bits (1042), Expect = 2.0e-109
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA
Sbjct: 95  RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 154

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS
Sbjct: 155 ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 214

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP
Sbjct: 215 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 274

Query: 181 EGSVIFGRRAVHTDGPNRTRGRVLNC 207
           EGSVIFGRRAVHTDGPNRTRGRVLNC
Sbjct: 275 EGSVIFGRRAVHTDGPNRTRGRVLNC 300

[Close]

BLAST of MS022464 vs. NCBI nr
Match: AQX45437.1 (WRKY transcription factor 1-like protein [Luffa aegyptiaca])

HSP 1 Score: 344.7 bits (883), Expect = 5.4e-91
Identity = 182/207 (87.92%), Postives = 191/207 (92.27%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPA KS HHHHHH+SSPS A+
Sbjct: 95  RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPATKS-HHHHHHNSSPSSAS 154

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           I AA A  DDSSPGSTTTSSSTSSGD+TN TPTSP+ AAKFEEAAAVFASQPELELGGDS
Sbjct: 155 IAAAVAVPDDSSPGSTTTSSSTSSGDNTNATPTSPT-AAKFEEAAAVFASQPELELGGDS 214

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           L+IKPC+GDFGW ADVAYDRILEGPICGGGDIFDDADVMVLSTR DD +ESLFADLGELP
Sbjct: 215 LMIKPCIGDFGWFADVAYDRILEGPICGGGDIFDDADVMVLSTRGDDEEESLFADLGELP 274

Query: 181 EGSVIFG-RRAVHTDGPNRTRGRVLNC 207
           EGSV+FG RRAV  DGPNRT GRVL+C
Sbjct: 275 EGSVVFGRRRAVEPDGPNRTCGRVLDC 299

[Close]

BLAST of MS022464 vs. NCBI nr
Match: XP_038888987.1 (probable WRKY transcription factor 69 isoform X1 [Benincasa hispida])

HSP 1 Score: 339.7 bits (870), Expect = 1.7e-89
Identity = 178/207 (85.99%), Postives = 188/207 (90.82%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDP+KLVITYAFDHNHQLP  KS  HHHHH+SSPS A 
Sbjct: 95  RGYYRCSSSKGCPARKQVERSRVDPSKLVITYAFDHNHQLPVTKS--HHHHHNSSPSSAV 154

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           I AASAAAD SSPGSTTTSSSTSSGD+TN  P+SP+ AAKFEEAAAVFASQPELELGGDS
Sbjct: 155 IAAASAAADSSSPGSTTTSSSTSSGDNTNAAPSSPAAAAKFEEAAAVFASQPELELGGDS 214

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           L+IKPC+GDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTR DD +ESLFADLGELP
Sbjct: 215 LMIKPCIGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRGDDEEESLFADLGELP 274

Query: 181 EGSVIFG-RRAVHTDGPNRTRGRVLNC 207
           EGSV+FG RR V  DGPNRT G VL+C
Sbjct: 275 EGSVVFGRRRPVQPDGPNRTCGTVLDC 299

[Close]

BLAST of MS022464 vs. NCBI nr
Match: XP_038888988.1 (probable WRKY transcription factor 69 isoform X2 [Benincasa hispida])

HSP 1 Score: 339.7 bits (870), Expect = 1.7e-89
Identity = 178/207 (85.99%), Postives = 188/207 (90.82%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDP+KLVITYAFDHNHQLP  KS  HHHHH+SSPS A 
Sbjct: 94  RGYYRCSSSKGCPARKQVERSRVDPSKLVITYAFDHNHQLPVTKS--HHHHHNSSPSSAV 153

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           I AASAAAD SSPGSTTTSSSTSSGD+TN  P+SP+ AAKFEEAAAVFASQPELELGGDS
Sbjct: 154 IAAASAAADSSSPGSTTTSSSTSSGDNTNAAPSSPAAAAKFEEAAAVFASQPELELGGDS 213

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           L+IKPC+GDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTR DD +ESLFADLGELP
Sbjct: 214 LMIKPCIGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRGDDEEESLFADLGELP 273

Query: 181 EGSVIFG-RRAVHTDGPNRTRGRVLNC 207
           EGSV+FG RR V  DGPNRT G VL+C
Sbjct: 274 EGSVVFGRRRPVQPDGPNRTCGTVLDC 298

[Close]

BLAST of MS022464 vs. ExPASy Swiss-Prot
Match: Q93WV5 (Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.8e-17
Identity = 70/201 (34.83%), Postives = 96/201 (47.76%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDP+KL+ITYA DHNH  P++ ++   HH  S      
Sbjct: 90  RGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFPSSSANTKSHHRSS------ 149

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           +   +A  ++               +   +T     PA              +L      
Sbjct: 150 VVLKTAKKEE---------EYEEEEEELTVTAAEEPPAGL------------DLSHVDSP 209

Query: 121 LLIKPC---LGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLG 180
           LL+  C   +G+FGW  D +           G   F D  +    +   + DESLF DLG
Sbjct: 210 LLLGGCYSEIGEFGWFYDASISS------SSGSSNFLDVTLERGFSVGQEEDESLFGDLG 257

Query: 181 ELPEGSVIFGRRAVHTDGPNR 199
           +LP+ + +F R  V T+  +R
Sbjct: 270 DLPDCASVFRRGTVATEEQHR 257

[Close]

BLAST of MS022464 vs. ExPASy Swiss-Prot
Match: Q9LP56 (Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.3e-17
Identity = 69/194 (35.57%), Postives = 87/194 (44.85%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSS+KGCPARKQVERSR DPT ++ITY  +HNH  P                   
Sbjct: 94  RGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWP------------------- 153

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
                             +SST +G      P  P P  + E  A        + LG   
Sbjct: 154 -----------------LTSSTRNGPKPKPEP-KPEPEPEVEPEAEE-EDNKFMVLGRGI 213

Query: 121 LLIKPCLGDFGWLADV--AYDRILEGPICGG--GDIFDDADVMVLSTRVDDVDESLFADL 180
                C+ +F W  ++      ILE PI           AD + +   + + DESLFADL
Sbjct: 214 ETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAVSGADDVAVFFPMGEEDESLFADL 249

Query: 181 GELPEGSVIFGRRA 191
           GELPE SV+F  R+
Sbjct: 274 GELPECSVVFRHRS 249

[Close]

BLAST of MS022464 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 3.7e-10
Identity = 46/116 (39.66%), Postives = 64/116 (55.17%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGC ARKQVERSR DP  LVITY  +HNH  P  +       +  + S  +
Sbjct: 237 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQR-------NALAGSTRS 296

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELEL 117
            T++S+  + S P +   +SS+    +T   P+S +P      +A       ++EL
Sbjct: 297 STSSSSNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMEL 345

[Close]

BLAST of MS022464 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 48/106 (45.28%), Postives = 59/106 (55.66%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPA- 60
           RGYYRCSSSKGC ARKQVERSR DP  LVITY  +HNH  P  ++        SS S   
Sbjct: 235 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLN 294

Query: 61  AITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAA 106
             + +S AA  +SP S    +++S  +  N    S S    F+ AA
Sbjct: 295 PSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAA 340

[Close]

BLAST of MS022464 vs. ExPASy Swiss-Prot
Match: Q9FL92 (Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.0e-07
Identity = 41/101 (40.59%), Postives = 56/101 (55.45%), Query Frame = 0


Query: 1    RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
            R YYRC+SSKGC ARKQVERSR DP   VITY  +HNH  P  +         ++ + + 
Sbjct: 1200 RSYYRCASSKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLR---------NTLAGST 1259

Query: 61   ITAASAAADDSSPGSTTTSSSTSSGD--HTNMTPTSPSPAA 100
             +++S  +D ++  S+T S      D  H   +P SP  AA
Sbjct: 1260 RSSSSKCSDVTTSASSTVSQDKEGPDKSHLPSSPASPPYAA 1291

[Close]

BLAST of MS022464 vs. ExPASy TrEMBL
Match: A0A6J1D1E0 (probable WRKY transcription factor 69 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016715 PE=4 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 9.5e-110
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA
Sbjct: 95  RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 154

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS
Sbjct: 155 ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 214

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP
Sbjct: 215 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 274

Query: 181 EGSVIFGRRAVHTDGPNRTRGRVLNC 207
           EGSVIFGRRAVHTDGPNRTRGRVLNC
Sbjct: 275 EGSVIFGRRAVHTDGPNRTRGRVLNC 300

[Close]

BLAST of MS022464 vs. ExPASy TrEMBL
Match: A0A6J1D2C7 (probable WRKY transcription factor 69 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016715 PE=4 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 9.5e-110
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA
Sbjct: 96  RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 155

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS
Sbjct: 156 ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 215

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP
Sbjct: 216 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 275

Query: 181 EGSVIFGRRAVHTDGPNRTRGRVLNC 207
           EGSVIFGRRAVHTDGPNRTRGRVLNC
Sbjct: 276 EGSVIFGRRAVHTDGPNRTRGRVLNC 301

[Close]

BLAST of MS022464 vs. ExPASy TrEMBL
Match: A0A1S6YJF1 (WRKY transcription factor 1-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.6e-91
Identity = 182/207 (87.92%), Postives = 191/207 (92.27%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPA KS HHHHHH+SSPS A+
Sbjct: 95  RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPATKS-HHHHHHNSSPSSAS 154

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           I AA A  DDSSPGSTTTSSSTSSGD+TN TPTSP+ AAKFEEAAAVFASQPELELGGDS
Sbjct: 155 IAAAVAVPDDSSPGSTTTSSSTSSGDNTNATPTSPT-AAKFEEAAAVFASQPELELGGDS 214

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           L+IKPC+GDFGW ADVAYDRILEGPICGGGDIFDDADVMVLSTR DD +ESLFADLGELP
Sbjct: 215 LMIKPCIGDFGWFADVAYDRILEGPICGGGDIFDDADVMVLSTRGDDEEESLFADLGELP 274

Query: 181 EGSVIFG-RRAVHTDGPNRTRGRVLNC 207
           EGSV+FG RRAV  DGPNRT GRVL+C
Sbjct: 275 EGSVVFGRRRAVEPDGPNRTCGRVLDC 299

[Close]

BLAST of MS022464 vs. ExPASy TrEMBL
Match: A0A6J1GKP7 (probable WRKY transcription factor 69 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454826 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.5e-88
Identity = 180/207 (86.96%), Postives = 188/207 (90.82%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPA KS  HHHHH+SSPS AA
Sbjct: 94  RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPATKS--HHHHHNSSPSSAA 153

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           I   +AA D SSPGSTTTSSSTSSGDHTN  P S S AAKFEEAAAVFASQPELELGGDS
Sbjct: 154 I---AAAPDSSSPGSTTTSSSTSSGDHTNTDP-SLSAAAKFEEAAAVFASQPELELGGDS 213

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           L+IKPC+GDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTR +D DESLFADLGELP
Sbjct: 214 LMIKPCIGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRGNDEDESLFADLGELP 273

Query: 181 EGSVIFG-RRAVHTDGPNRTRGRVLNC 207
           EGS++FG RRAV  DGPNRTRGRVL+C
Sbjct: 274 EGSMVFGRRRAVQPDGPNRTRGRVLDC 294

[Close]

BLAST of MS022464 vs. ExPASy TrEMBL
Match: A0A6J1GKJ3 (probable WRKY transcription factor 69 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454826 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.5e-88
Identity = 180/207 (86.96%), Postives = 188/207 (90.82%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPA KS  HHHHH+SSPS AA
Sbjct: 95  RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPATKS--HHHHHNSSPSSAA 154

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           I   +AA D SSPGSTTTSSSTSSGDHTN  P S S AAKFEEAAAVFASQPELELGGDS
Sbjct: 155 I---AAAPDSSSPGSTTTSSSTSSGDHTNTDP-SLSAAAKFEEAAAVFASQPELELGGDS 214

Query: 121 LLIKPCLGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLGELP 180
           L+IKPC+GDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTR +D DESLFADLGELP
Sbjct: 215 LMIKPCIGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRGNDEDESLFADLGELP 274

Query: 181 EGSVIFG-RRAVHTDGPNRTRGRVLNC 207
           EGS++FG RRAV  DGPNRTRGRVL+C
Sbjct: 275 EGSMVFGRRRAVQPDGPNRTRGRVLDC 295

[Close]

BLAST of MS022464 vs. TAIR 10
Match: AT3G58710.1 (WRKY DNA-binding protein 69 )

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-18
Identity = 70/201 (34.83%), Postives = 96/201 (47.76%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDP+KL+ITYA DHNH  P++ ++   HH  S      
Sbjct: 91  RGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFPSSSANTKSHHRSS------ 150

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           +   +A  ++               +   +T     PA              +L      
Sbjct: 151 VVLKTAKKEE---------EYEEEEEELTVTAAEEPPAGL------------DLSHVDSP 210

Query: 121 LLIKPC---LGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLG 180
           LL+  C   +G+FGW  D +           G   F D  +    +   + DESLF DLG
Sbjct: 211 LLLGGCYSEIGEFGWFYDASISS------SSGSSNFLDVTLERGFSVGQEEDESLFGDLG 258

Query: 181 ELPEGSVIFGRRAVHTDGPNR 199
           +LP+ + +F R  V T+  +R
Sbjct: 271 DLPDCASVFRRGTVATEEQHR 258

[Close]

BLAST of MS022464 vs. TAIR 10
Match: AT3G58710.2 (WRKY DNA-binding protein 69 )

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-18
Identity = 70/201 (34.83%), Postives = 96/201 (47.76%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGCPARKQVERSRVDP+KL+ITYA DHNH  P++ ++   HH  S      
Sbjct: 90  RGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFPSSSANTKSHHRSS------ 149

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
           +   +A  ++               +   +T     PA              +L      
Sbjct: 150 VVLKTAKKEE---------EYEEEEEELTVTAAEEPPAGL------------DLSHVDSP 209

Query: 121 LLIKPC---LGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRVDDVDESLFADLG 180
           LL+  C   +G+FGW  D +           G   F D  +    +   + DESLF DLG
Sbjct: 210 LLLGGCYSEIGEFGWFYDASISS------SSGSSNFLDVTLERGFSVGQEEDESLFGDLG 257

Query: 181 ELPEGSVIFGRRAVHTDGPNR 199
           +LP+ + +F R  V T+  +R
Sbjct: 270 DLPDCASVFRRGTVATEEQHR 257

[Close]

BLAST of MS022464 vs. TAIR 10
Match: AT1G29280.1 (WRKY DNA-binding protein 65 )

HSP 1 Score: 89.4 bits (220), Expect = 3.8e-18
Identity = 69/194 (35.57%), Postives = 87/194 (44.85%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSS+KGCPARKQVERSR DPT ++ITY  +HNH  P                   
Sbjct: 94  RGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWP------------------- 153

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELELGGDS 120
                             +SST +G      P  P P  + E  A        + LG   
Sbjct: 154 -----------------LTSSTRNGPKPKPEP-KPEPEPEVEPEAEE-EDNKFMVLGRGI 213

Query: 121 LLIKPCLGDFGWLADV--AYDRILEGPICGG--GDIFDDADVMVLSTRVDDVDESLFADL 180
                C+ +F W  ++      ILE PI           AD + +   + + DESLFADL
Sbjct: 214 ETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAVSGADDVAVFFPMGEEDESLFADL 249

Query: 181 GELPEGSVIFGRRA 191
           GELPE SV+F  R+
Sbjct: 274 GELPECSVVFRHRS 249

[Close]

BLAST of MS022464 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 66.6 bits (161), Expect = 2.6e-11
Identity = 46/116 (39.66%), Postives = 64/116 (55.17%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPAA 60
           RGYYRCSSSKGC ARKQVERSR DP  LVITY  +HNH  P  +       +  + S  +
Sbjct: 237 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQR-------NALAGSTRS 296

Query: 61  ITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAAAVFASQPELEL 117
            T++S+  + S P +   +SS+    +T   P+S +P      +A       ++EL
Sbjct: 297 STSSSSNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMEL 345

[Close]

BLAST of MS022464 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 61.6 bits (148), Expect = 8.4e-10
Identity = 48/106 (45.28%), Postives = 59/106 (55.66%), Query Frame = 0


Query: 1   RGYYRCSSSKGCPARKQVERSRVDPTKLVITYAFDHNHQLPAAKSHHHHHHHHSSPSPA- 60
           RGYYRCSSSKGC ARKQVERSR DP  LVITY  +HNH  P  ++        SS S   
Sbjct: 235 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLN 294

Query: 61  AITAASAAADDSSPGSTTTSSSTSSGDHTNMTPTSPSPAAKFEEAA 106
             + +S AA  +SP S    +++S  +  N    S S    F+ AA
Sbjct: 295 PSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAA 340

The following BLAST results are available for this feature:

BLAST of MS022464 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147889.1	2.0e-109	100.00	probable WRKY transcription factor 69 isoform X1 [Momordica charantia]	[more]
XP_022147890.1	2.0e-109	100.00	probable WRKY transcription factor 69 isoform X2 [Momordica charantia]	[more]
AQX45437.1	5.4e-91	87.92	WRKY transcription factor 1-like protein [Luffa aegyptiaca]	[more]
XP_038888987.1	1.7e-89	85.99	probable WRKY transcription factor 69 isoform X1 [Benincasa hispida]	[more]
XP_038888988.1	1.7e-89	85.99	probable WRKY transcription factor 69 isoform X2 [Benincasa hispida]	[more]

BLAST of MS022464 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WV5	1.8e-17	34.83	Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 ...	[more]
Q9LP56	5.3e-17	35.57	Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 ...	[more]
Q9SA80	3.7e-10	39.66	Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ...	[more]
O64747	1.2e-08	45.28	Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ...	[more]
Q9FL92	5.0e-07	40.59	Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 S...	[more]

BLAST of MS022464 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1E0	9.5e-110	100.00	probable WRKY transcription factor 69 isoform X2 OS=Momordica charantia OX=3673 ...	[more]
A0A6J1D2C7	9.5e-110	100.00	probable WRKY transcription factor 69 isoform X1 OS=Momordica charantia OX=3673 ...	[more]
A0A1S6YJF1	2.6e-91	87.92	WRKY transcription factor 1-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1GKP7	3.5e-88	86.96	probable WRKY transcription factor 69 isoform X2 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1GKJ3	3.5e-88	86.96	probable WRKY transcription factor 69 isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]

BLAST of MS022464 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58710.1	1.3e-18	34.83	WRKY DNA-binding protein 69	[more]
AT3G58710.2	1.3e-18	34.83	WRKY DNA-binding protein 69	[more]
AT1G29280.1	3.8e-18	35.57	WRKY DNA-binding protein 65	[more]
AT1G30650.1	2.6e-11	39.66	WRKY DNA-binding protein 14	[more]
AT2G34830.1	8.4e-10	45.28	WRKY DNA-binding protein 35	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 1..40 e-value: 8.0E-8 score: 39.1
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 1..39 e-value: 4.7E-12 score: 46.0
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 1..41 score: 19.301605
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 1..42 e-value: 6.8E-14 score: 53.4
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 1..41
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..97
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 68..96
None	No IPR available	PANTHER	PTHR32096:SF19	OS01G0750100 PROTEIN	coord: 1..190
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR32096	WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED	coord: 1..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS022464.1	MS022464.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding