MS022461 (gene) Bitter gourd (TR) v1

Overview


Name	MS022461
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	E3 ubiquitin-protein ligase RNF12-like isoform X1
Location	scaffold47: 3580296 .. 3581887 (+)
RNA-Seq Expression	MS022461
Synteny	MS022461

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAGACGCTCTACTAATTTTCCTCCAGGAATTTGGCAGGCTAGAAGGGAGTATGGCCGCCAGAGACATAACATAAATTATTCAGACCATGTGAACTCCACGCCGCCACACTCCCATTCATTTCAGGTCAGCAGGTTAACAAATTCTATTGTTCCGCAACGAACTGGATTCAAACAGAATTTGCGGGCAGCAAAACAGAATTTGCAGATCACTTTTAATTCCTTATTAATATGAGAGTTCTCCTAATTTTTCTCAGAAATATGTTAGTAGTCCCGAAGTTAAAGAGATGATAACAAGCGTATTACTCATTATGCATGTGTTGTCAGAATTCTTATATAGAGAGAGATCCATTTCCATGGCGGCCTGCAGGGGAGGCAATGTTTGCAAGAATATTGGACATTGGAAGGTAAATAACACAAAAGCTTTGAAGTTTTATATGTGCTGTTTAGTGTTTTGGAAGAGTTATATGCATACCATAGAAGTGGATATTGCAGATTCATTTCTTAAATGATCTGAAATTATAAGAATTAGAGGTCAATCTAAGTTTGCTGTATGTTTCTCAAAACATGAACTAGTATGTAACTCAGGGAGATAACTCGACAGAGCAATCAGAAGTCCATGAATTTCACTAATGGGAGAATTTGAGGAAACAAATCATGCAAACCATGAAGTTTCAAAAATGGGGGTTGAGCACCAGTTCCTTCTTAGTTGGAGATTGGTTAACACAAGACAGTTCAACGACATTCAACACAATTCATTAAGCAAAAATTTGTTCAACAACCACCTCTATTTACAAGTTGCAGGGAAAAAGAAACATGTGTTCTCATTCATTTGATTGAATAGCTTATGATATAACATCACTCAATTTTTGTCATGAGCAGCATCAAATTCTGCAATAACAAACTAATCCCCTTTGAATACCGGTTGGTTTCAGAGAATCAACTTACATTGCTTTTATTACCTGTAAGTAAAATCCATGTCCTCCGAACTGTGTTGATATTTCATTCATTTTTTGAGAAATTTCCAGCCCTCCGGAAGAAACACCTACCATTGTCAGAATCATTACCCACTCCAGACCGGGACAGCTCGAATCCAGTCCAAGACCTGCGGAACTGTACACGCCAACTCCAAGATCCCAAGGGAACTCGACGCTAACATCTGAAGAGCAAAATATGGCACTGAATAAGCTCAAGAAAAAGATCTATGATCCTCCGAAGAACCTAGGTAAGAGAATTAACTTCTACTACAGAGAAAATTACAAGGAGAGGAGCAACGGGGAGAGCGATGAAGACGGCAAGAGATGCTCCGTTTGCCTTGAAGATTTCCAGCCGAGAGAGGAGGTGATGACGACGCCCTGCAACCATATGTTCCACGAAGAATGCATCGTGCCGTGGGTGAAGAGCAATGGCCAATGTCCGGTCTGCAGATTCGCTTTGTTCGAGCGAACAGGGCGAAGGGCGGCTTCATCCAACAACGCTACTGCATCAAATGTTCTGACCAATAATCTACCCGACGATGAGATCGTGTACTTAATACGAGCCATGGAAGAAGCTCTGATGCTGCGAAATCTCGCCGGATTTTGGGGAGCA

mRNA sequence

ATGAGCAGACGCTCTACTAATTTTCCTCCAGGAATTTGGCAGGCTAGAAGGGAGTATGGCCGCCAGAGACATAACATAAATTATTCAGACCATGTGAACTCCACGCCGCCACACTCCCATTCATTTCAGAATTCTTATATAGAGAGAGATCCATTTCCATGGCGGCCTGCAGGGGAGGCAATGTTTGCAAGAATATTGGACATTGGAAGCCCTCCGGAAGAAACACCTACCATTGTCAGAATCATTACCCACTCCAGACCGGGACAGCTCGAATCCAGTCCAAGACCTGCGGAACTGTACACGCCAACTCCAAGATCCCAAGGGAACTCGACGCTAACATCTGAAGAGCAAAATATGGCACTGAATAAGCTCAAGAAAAAGATCTATGATCCTCCGAAGAACCTAGGTAAGAGAATTAACTTCTACTACAGAGAAAATTACAAGGAGAGGAGCAACGGGGAGAGCGATGAAGACGGCAAGAGATGCTCCGTTTGCCTTGAAGATTTCCAGCCGAGAGAGGAGGTGATGACGACGCCCTGCAACCATATGTTCCACGAAGAATGCATCGTGCCGTGGGTGAAGAGCAATGGCCAATGTCCGGTCTGCAGATTCGCTTTGTTCGAGCGAACAGGGCGAAGGGCGGCTTCATCCAACAACGCTACTGCATCAAATGTTCTGACCAATAATCTACCCGACGATGAGATCGTGTACTTAATACGAGCCATGGAAGAAGCTCTGATGCTGCGAAATCTCGCCGGATTTTGGGGAGCA

Coding sequence (CDS)

ATGAGCAGACGCTCTACTAATTTTCCTCCAGGAATTTGGCAGGCTAGAAGGGAGTATGGCCGCCAGAGACATAACATAAATTATTCAGACCATGTGAACTCCACGCCGCCACACTCCCATTCATTTCAGAATTCTTATATAGAGAGAGATCCATTTCCATGGCGGCCTGCAGGGGAGGCAATGTTTGCAAGAATATTGGACATTGGAAGCCCTCCGGAAGAAACACCTACCATTGTCAGAATCATTACCCACTCCAGACCGGGACAGCTCGAATCCAGTCCAAGACCTGCGGAACTGTACACGCCAACTCCAAGATCCCAAGGGAACTCGACGCTAACATCTGAAGAGCAAAATATGGCACTGAATAAGCTCAAGAAAAAGATCTATGATCCTCCGAAGAACCTAGGTAAGAGAATTAACTTCTACTACAGAGAAAATTACAAGGAGAGGAGCAACGGGGAGAGCGATGAAGACGGCAAGAGATGCTCCGTTTGCCTTGAAGATTTCCAGCCGAGAGAGGAGGTGATGACGACGCCCTGCAACCATATGTTCCACGAAGAATGCATCGTGCCGTGGGTGAAGAGCAATGGCCAATGTCCGGTCTGCAGATTCGCTTTGTTCGAGCGAACAGGGCGAAGGGCGGCTTCATCCAACAACGCTACTGCATCAAATGTTCTGACCAATAATCTACCCGACGATGAGATCGTGTACTTAATACGAGCCATGGAAGAAGCTCTGATGCTGCGAAATCTCGCCGGATTTTGGGGAGCA

Protein sequence

MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEAMFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMALNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRCSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATASNVLTNNLPDDEIVYLIRAMEEALMLRNLAGFWGA

Homology

[Close]

BLAST of MS022461 vs. NCBI nr
Match: XP_022147817.1 (E3 ubiquitin-protein ligase RNF12-like isoform X1 [Momordica charantia])

HSP 1 Score: 535.0 bits (1377), Expect = 3.5e-148
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60
           MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA
Sbjct: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60

Query: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120
           MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA
Sbjct: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120

Query: 121 LNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRCSVCLEDFQPREEVMTTPC 180
           LNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRCSVCLEDFQPREEVMTTPC
Sbjct: 121 LNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRCSVCLEDFQPREEVMTTPC 180

Query: 181 NHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATASNVLTNNLPDDEIVYLIR 240
           NHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATASNVLTNNLPDDEIVYLIR
Sbjct: 181 NHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATASNVLTNNLPDDEIVYLIR 240

Query: 241 AMEEALMLRNLAGFWGA 258
           AMEEALMLRNLAGFWGA
Sbjct: 241 AMEEALMLRNLAGFWGA 257

[Close]

BLAST of MS022461 vs. NCBI nr
Match: XP_022147818.1 (E3 ubiquitin-protein ligase RNF12-B-like isoform X2 [Momordica charantia])

HSP 1 Score: 446.0 bits (1146), Expect = 2.1e-121
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 43  QNSYIERDPFPWRPAGEAMFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTP 102
           QNSYIERDPFPWRPAGEAMFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTP
Sbjct: 4   QNSYIERDPFPWRPAGEAMFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTP 63

Query: 103 TPRSQGNSTLTSEEQNMALNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRC 162
           TPRSQGNSTLTSEEQNMALNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRC
Sbjct: 64  TPRSQGNSTLTSEEQNMALNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRC 123

Query: 163 SVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATA 222
           SVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATA
Sbjct: 124 SVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATA 183

Query: 223 SNVLTNNLPDDEIVYLIRAMEEALMLRNLAGFWGA 258
           SNVLTNNLPDDEIVYLIRAMEEALMLRNLAGFWGA
Sbjct: 184 SNVLTNNLPDDEIVYLIRAMEEALMLRNLAGFWGA 218

[Close]

BLAST of MS022461 vs. NCBI nr
Match: XP_038888228.1 (uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida])

HSP 1 Score: 409.5 bits (1051), Expect = 2.2e-110
Identity = 201/262 (76.72%), Postives = 222/262 (84.73%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60
           MSRRSTNFPPGIWQARREY RQR+N N S+ +NSTP H++SFQNSY E DP PWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRINSTPSHTNSFQNSYREVDPVPWRPAGET 60

Query: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120
           M ARILDIG P EE P IVRIIT SRP QLE+SP PAELY+PTPRS+GNSTLTSEEQNMA
Sbjct: 61  MIARILDIGRPREEPPAIVRIITSSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKIYDPPKNLGKRINFYYRE----NYKERSNGESDEDGKRCSVCLEDFQPREEVM 180
           L KLKK +YDPP N  +RI+FYYRE    NY ER+N  SDE+GKRC+VCLEDFQP+EEVM
Sbjct: 121 LTKLKKNVYDPPSNTSRRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSN-NATASNVLTNNLPDDEI 240
           TTPCNHMFHEECIVPWVKSNGQCPVCRF LFERT RR ASSN N T S    ++L DD++
Sbjct: 181 TTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTERRGASSNSNNTVSTAPPSSLIDDDL 240

Query: 241 VYLIRAMEEALMLRNLAGFWGA 258
           +YLIRAME+ALMLRNLAGFWGA
Sbjct: 241 MYLIRAMEDALMLRNLAGFWGA 262

[Close]

BLAST of MS022461 vs. NCBI nr
Match: XP_008455285.1 (PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo])

HSP 1 Score: 404.8 bits (1039), Expect = 5.4e-109
Identity = 202/263 (76.81%), Postives = 220/263 (83.65%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60
           MSRRSTNFPPGIWQARREY RQR+N N S+ VNSTP H+ SFQNSY E DP PWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120
           M  RILDIGS PEE P IVRIITHSRP Q+E+SP PAELY+PTPRS+GNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKIYDPPKNLGKRINFYYRE----NYKERSNGESDEDGKRCSVCLEDFQPREEVM 180
           LNKLKKK+YDPP N  +RINFYYRE    NY ER N  SDE+GK C+VCLEDFQP+EEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTGRRAASSN-NATASNVLTNNLPDDE 240
           TTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RT RR ASSN N   S    ++L DDE
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNNVSIAPPSSLVDDE 240

Query: 241 IVYLIRAMEEALMLRNLAGFWGA 258
           ++YLIRAMEEALMLRN+A FWGA
Sbjct: 241 LMYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of MS022461 vs. NCBI nr
Match: TYK07001.1 (E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 401.4 bits (1030), Expect = 6.0e-108
Identity = 200/261 (76.63%), Postives = 219/261 (83.91%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60
           MSRRSTNFPPGIWQARREY RQR+N N S+ VNSTP H+ SFQNSY E DP PWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120
           M  RILDIGS PEE P IVRIITHSRP Q+E+SP PAELY+PTPRS+GNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKIYDPPKNLGKRINFYYRE----NYKERSNGESDEDGKRCSVCLEDFQPREEVM 180
           LNKLKKK+YDPP N  +RINFYYRE    NY ER N  SDE+GK C+VCLEDFQP+EEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTGRRAASSN-NATASNVLTNNLPDDE 240
           TTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RT RR ASSN N + S    ++L DDE
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNSVSIAPPSSLVDDE 240

Query: 241 IVYLIRAMEEALMLRNLAGFW 256
           ++YLIRAMEEALMLRN+A FW
Sbjct: 241 LMYLIRAMEEALMLRNVAEFW 261

[Close]

BLAST of MS022461 vs. ExPASy Swiss-Prot
Match: Q9Y4L5 (E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 5.8e-13
Identity = 34/76 (44.74%), Postives = 44/76 (57.89%), Query Frame = 0


Query: 154 ESDEDGKRCSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRR 213
           E  + G  C VC ED+   EEV   PCNH FH  CIVPW++ +  CPVCR +L      R
Sbjct: 220 EQVDMGLECPVCKEDYTVEEEVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTR 279

Query: 214 AASSNNATASNVLTNN 230
            + S  A+ASN  +N+
Sbjct: 280 QSQSTEASASNRFSND 295

[Close]

BLAST of MS022461 vs. ExPASy Swiss-Prot
Match: Q9D0C1 (E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.7e-12
Identity = 33/76 (43.42%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 154 ESDEDGKRCSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRR 213
           E    G  C VC ED+   E+V   PCNH FH  CIVPW++ +  CPVCR +L      R
Sbjct: 221 EQVNTGLECPVCKEDYTVEEKVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTR 280

Query: 214 AASSNNATASNVLTNN 230
              S+ A+ASN  +N+
Sbjct: 281 QTQSSEASASNRFSND 296

[Close]

BLAST of MS022461 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 9.3e-11
Identity = 28/70 (40.00%), Postives = 38/70 (54.29%), Query Frame = 0


Query: 162 CSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNAT 221
           C+VC E F+   E    PC H+FH++CIVPW+     CPVCRF L     RR+ ++    
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDN 258

Query: 222 ASNVLTNNLP 232
           A  +    LP
Sbjct: 259 AVGMTIWRLP 268

[Close]

BLAST of MS022461 vs. ExPASy Swiss-Prot
Match: Q91YL2 (E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.6e-10
Identity = 28/59 (47.46%), Postives = 38/59 (64.41%), Query Frame = 0


Query: 159 GKRCSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASS 218
           G  C VC ED+   E V   PCNH+FH+ CIVPW++ +  CPVCR +L   TG+  A++
Sbjct: 228 GLECPVCKEDYALGESVRQLPCNHLFHDSCIVPWLEQHDSCPVCRKSL---TGQNTATN 283

[Close]

BLAST of MS022461 vs. ExPASy Swiss-Prot
Match: Q6IRP0 (E3 ubiquitin-protein ligase RNF126-B OS=Xenopus laevis OX=8355 GN=rnf126-b PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.7e-10
Identity = 31/87 (35.63%), Postives = 43/87 (49.43%), Query Frame = 0


Query: 159 GKRCSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL------------ 218
           G  C VC ED+   E V   PCNH+FH +CI+PW++ +  CPVCR +L            
Sbjct: 225 GLECPVCKEDYTVGESVRQLPCNHLFHNDCIIPWLEQHDTCPVCRKSLSGQNTATNPPGL 284

Query: 219 ----FERTGRRAASSNNATASNVLTNN 230
               F  +   ++SS + T  N   NN
Sbjct: 285 TDMTFSSSSTSSSSSTSPTDENNTANN 311

[Close]

BLAST of MS022461 vs. ExPASy TrEMBL
Match: A0A6J1D3E4 (E3 ubiquitin-protein ligase RNF12-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016663 PE=4 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 1.7e-148
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60
           MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA
Sbjct: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60

Query: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120
           MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA
Sbjct: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120

Query: 121 LNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRCSVCLEDFQPREEVMTTPC 180
           LNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRCSVCLEDFQPREEVMTTPC
Sbjct: 121 LNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRCSVCLEDFQPREEVMTTPC 180

Query: 181 NHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATASNVLTNNLPDDEIVYLIR 240
           NHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATASNVLTNNLPDDEIVYLIR
Sbjct: 181 NHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATASNVLTNNLPDDEIVYLIR 240

Query: 241 AMEEALMLRNLAGFWGA 258
           AMEEALMLRNLAGFWGA
Sbjct: 241 AMEEALMLRNLAGFWGA 257

[Close]

BLAST of MS022461 vs. ExPASy TrEMBL
Match: A0A6J1D2C6 (E3 ubiquitin-protein ligase RNF12-B-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016663 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.0e-121
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 43  QNSYIERDPFPWRPAGEAMFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTP 102
           QNSYIERDPFPWRPAGEAMFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTP
Sbjct: 4   QNSYIERDPFPWRPAGEAMFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTP 63

Query: 103 TPRSQGNSTLTSEEQNMALNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRC 162
           TPRSQGNSTLTSEEQNMALNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRC
Sbjct: 64  TPRSQGNSTLTSEEQNMALNKLKKKIYDPPKNLGKRINFYYRENYKERSNGESDEDGKRC 123

Query: 163 SVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATA 222
           SVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATA
Sbjct: 124 SVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNATA 183

Query: 223 SNVLTNNLPDDEIVYLIRAMEEALMLRNLAGFWGA 258
           SNVLTNNLPDDEIVYLIRAMEEALMLRNLAGFWGA
Sbjct: 184 SNVLTNNLPDDEIVYLIRAMEEALMLRNLAGFWGA 218

[Close]

BLAST of MS022461 vs. ExPASy TrEMBL
Match: A0A1S3C054 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495488 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.6e-109
Identity = 202/263 (76.81%), Postives = 220/263 (83.65%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60
           MSRRSTNFPPGIWQARREY RQR+N N S+ VNSTP H+ SFQNSY E DP PWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120
           M  RILDIGS PEE P IVRIITHSRP Q+E+SP PAELY+PTPRS+GNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKIYDPPKNLGKRINFYYRE----NYKERSNGESDEDGKRCSVCLEDFQPREEVM 180
           LNKLKKK+YDPP N  +RINFYYRE    NY ER N  SDE+GK C+VCLEDFQP+EEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTGRRAASSN-NATASNVLTNNLPDDE 240
           TTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RT RR ASSN N   S    ++L DDE
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNNVSIAPPSSLVDDE 240

Query: 241 IVYLIRAMEEALMLRNLAGFWGA 258
           ++YLIRAMEEALMLRN+A FWGA
Sbjct: 241 LMYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of MS022461 vs. ExPASy TrEMBL
Match: A0A5D3C526 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003170 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.9e-108
Identity = 200/261 (76.63%), Postives = 219/261 (83.91%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60
           MSRRSTNFPPGIWQARREY RQR+N N S+ VNSTP H+ SFQNSY E DP PWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120
           M  RILDIGS PEE P IVRIITHSRP Q+E+SP PAELY+PTPRS+GNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKIYDPPKNLGKRINFYYRE----NYKERSNGESDEDGKRCSVCLEDFQPREEVM 180
           LNKLKKK+YDPP N  +RINFYYRE    NY ER N  SDE+GK C+VCLEDFQP+EEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTGRRAASSN-NATASNVLTNNLPDDE 240
           TTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RT RR ASSN N + S    ++L DDE
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNSVSIAPPSSLVDDE 240

Query: 241 IVYLIRAMEEALMLRNLAGFW 256
           ++YLIRAMEEALMLRN+A FW
Sbjct: 241 LMYLIRAMEEALMLRNVAEFW 261

[Close]

BLAST of MS022461 vs. ExPASy TrEMBL
Match: A0A5A7SLQ9 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003130 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.5e-107
Identity = 199/261 (76.25%), Postives = 218/261 (83.52%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYGRQRHNINYSDHVNSTPPHSHSFQNSYIERDPFPWRPAGEA 60
           MSRRSTNFPPGIWQARREY RQR+N N S+ VNSTP H+ SFQNSY E DP PWRPAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  MFARILDIGSPPEETPTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMA 120
           M  RILDIGS PEE P IVRIITHSRP Q+E+SP PAELY+PTPRS+GNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKIYDPPKNLGKRINFYYRE----NYKERSNGESDEDGKRCSVCLEDFQPREEVM 180
           LNKLKKK+YDPP N  +RINFYYRE    NY ER N  SDE+GK C+VCLEDFQ +EEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQSKEEVM 180

Query: 181 TTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTGRRAASSN-NATASNVLTNNLPDDE 240
           TTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RT RR ASSN N + S    ++L DDE
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNSVSIAPPSSLVDDE 240

Query: 241 IVYLIRAMEEALMLRNLAGFW 256
           ++YLIRAMEEALMLRN+A FW
Sbjct: 241 LMYLIRAMEEALMLRNVAEFW 261

[Close]

BLAST of MS022461 vs. TAIR 10
Match: AT3G58720.2 (RING/U-box superfamily protein )

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-30
Identity = 80/223 (35.87%), Postives = 122/223 (54.71%), Query Frame = 0


Query: 39  SHSFQNSYIERDPFPWRPAGEAMFARILDIGSPPEETPTI-VRIITHSRPGQLESSPRPA 98
           S+S  N ++  DP           +  +D+  P      I +R   + R   LE+  +  
Sbjct: 42  SNSIVNRHLIDDPI--SAYSRTSISSTVDLVPPSHTRHQIPIRNSNNLRRQHLEALRQAV 101

Query: 99  ELYTPTPRSQGNSTLTSEEQNMALNKLKKKIYDP-PKN-LGKRINFYYR------ENYKE 158
              +  P S   S+  + E++  L  L K+ Y+P PK+ L + ++ YYR      E+ + 
Sbjct: 102 HNQSTRPESSSRSSSQTNEEDEILKHLTKETYNPVPKSTLLRSLSLYYRNKNPGSESSRN 161

Query: 159 RSNGESDEDGKRCSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFER 218
             N   +ED KRCSVCLEDF+P+E VM TPC HMFHEECIVPW+K+ GQCPVCRF + + 
Sbjct: 162 TQNLSGEEDDKRCSVCLEDFEPKETVMLTPCKHMFHEECIVPWLKTKGQCPVCRFVILKP 221

Query: 219 TGRRAASSNNAT--ASNVLTNNLPDDEIVYLIRAMEEALMLRN 251
           T + ++   N +    ++ TN+L   E++ ++RAMEE  +  N
Sbjct: 222 TKQDSSPPANGSNLTGDMTTNDLFTLELLSVVRAMEETFLFGN 262

[Close]

BLAST of MS022461 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 2.4e-30
Identity = 75/192 (39.06%), Postives = 111/192 (57.81%), Query Frame = 0


Query: 71  PPEET--PTIVRIITHSRPGQLESSPRPAELYTPTPRSQGNSTLTSEEQNMALNKLKKKI 130
           PP  T     +R   + R   LE+  +     +  P S   S+  + E++  L  L K+ 
Sbjct: 43  PPSHTRHQIPIRNSNNLRRQHLEALRQAVHNQSTRPESSSRSSSQTNEEDEILKHLTKET 102

Query: 131 YDP-PKN-LGKRINFYYR------ENYKERSNGESDEDGKRCSVCLEDFQPREEVMTTPC 190
           Y+P PK+ L + ++ YYR      E+ +   N   +ED KRCSVCLEDF+P+E VM TPC
Sbjct: 103 YNPVPKSTLLRSLSLYYRNKNPGSESSRNTQNLSGEEDDKRCSVCLEDFEPKETVMLTPC 162

Query: 191 NHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNAT--ASNVLTNNLPDDEIVYL 250
            HMFHEECIVPW+K+ GQCPVCRF + + T + ++   N +    ++ TN+L   E++ +
Sbjct: 163 KHMFHEECIVPWLKTKGQCPVCRFVILKPTKQDSSPPANGSNLTGDMTTNDLFTLELLSV 222

[Close]

BLAST of MS022461 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 68.9 bits (167), Expect = 6.6e-12
Identity = 28/70 (40.00%), Postives = 38/70 (54.29%), Query Frame = 0


Query: 162 CSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTGRRAASSNNAT 221
           C+VC E F+   E    PC H+FH++CIVPW+     CPVCRF L     RR+ ++    
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDN 258

Query: 222 ASNVLTNNLP 232
           A  +    LP
Sbjct: 259 AVGMTIWRLP 268

[Close]

BLAST of MS022461 vs. TAIR 10
Match: AT2G44330.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.5e-11
Identity = 24/56 (42.86%), Postives = 35/56 (62.50%), Query Frame = 0


Query: 151 SNGESDEDGKRCSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL 207
           S+  SD+    C++C EDF   E     PCNH++H +CI+PW+ S+  CP+CR  L
Sbjct: 85  SSASSDDSALPCAICREDFVVGESARRLPCNHLYHNDCIIPWLTSHNSCPLCRVEL 140

[Close]

BLAST of MS022461 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.9e-11
Identity = 25/46 (54.35%), Postives = 31/46 (67.39%), Query Frame = 0


Query: 161 RCSVCLEDFQPREEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL 207
           +CSVCL+DF+   E    PC H FH  CIVPW++ +  CPVCRF L
Sbjct: 258 QCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLELHSSCPVCRFEL 303

The following BLAST results are available for this feature:

BLAST of MS022461 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147817.1	3.5e-148	100.00	E3 ubiquitin-protein ligase RNF12-like isoform X1 [Momordica charantia]	[more]
XP_022147818.1	2.1e-121	100.00	E3 ubiquitin-protein ligase RNF12-B-like isoform X2 [Momordica charantia]	[more]
XP_038888228.1	2.2e-110	76.72	uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida]	[more]
XP_008455285.1	5.4e-109	76.81	PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo]	[more]
TYK07001.1	6.0e-108	76.63	E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo var. makuwa]	[more]

BLAST of MS022461 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y4L5	5.8e-13	44.74	E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2	[more]
Q9D0C1	1.7e-12	43.42	E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1	[more]
Q940T5	9.3e-11	40.00	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q91YL2	1.6e-10	47.46	E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1	[more]
Q6IRP0	2.7e-10	35.63	E3 ubiquitin-protein ligase RNF126-B OS=Xenopus laevis OX=8355 GN=rnf126-b PE=2 ...	[more]

BLAST of MS022461 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D3E4	1.7e-148	100.00	E3 ubiquitin-protein ligase RNF12-like isoform X1 OS=Momordica charantia OX=3673...	[more]
A0A6J1D2C6	1.0e-121	100.00	E3 ubiquitin-protein ligase RNF12-B-like isoform X2 OS=Momordica charantia OX=36...	[more]
A0A1S3C054	2.6e-109	76.81	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A5D3C526	2.9e-108	76.63	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A5A7SLQ9	2.5e-107	76.25	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194...	[more]

BLAST of MS022461 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58720.2	1.1e-30	35.87	RING/U-box superfamily protein	[more]
AT3G58720.1	2.4e-30	39.06	RING/U-box superfamily protein	[more]
AT5G59550.1	6.6e-12	40.00	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G44330.1	1.5e-11	42.86	RING/U-box superfamily protein	[more]
AT5G56340.1	1.9e-11	54.35	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 162..202 e-value: 2.1E-9 score: 47.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 161..203 e-value: 3.3E-14 score: 52.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 162..203 score: 13.507235
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 146..220 e-value: 1.6E-21 score: 77.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 99..113
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..113
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 117..250
None	No IPR available	PANTHER	PTHR22765:SF328	ANAPHASE-PROMOTING COMPLEX SUBUNIT 11 RING-H2 FINGER PROTEIN	coord: 117..250
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 153..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS022461.1	MS022461.1	mRNA