MS022246 (gene) Bitter gourd (TR) v1

Overview
NameMS022246
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionHomoserine kinase
Locationscaffold47: 1995094 .. 1996194 (+)
RNA-Seq ExpressionMS022246
SyntenyMS022246
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATCTGCTTTCAGCCAACATTGAAGCCGGTGGCGGTTCCTCCAGTTTCTCTATCTAACCCTAAATCTGTCCTCTTCAGGTGCAATTTGTCTCTACCATCCAGGACCGCCGTCACTACGGTCGAACCTGAACCTGTATTCTCCTCTGTCAAAGCATTCGCACCTGCAACCGTCGCTAATTTAGGCCCTGGCTTCGATTTCCTCGGCTGCGCTGTCGATGGACTCGGCGATTTCGTCTCCCTCAGCGTTGATTCCAATGTTCATCCTGGTGAGGTCGCAATCACCAATATTTCTGGCAATAACACGAATAAGCTTAGTAAAAACCCTTTGTACAATTGCGCGGGCATTGCCGCTATCGAGGTCATGAAAATGCTAGGGATCCGATCTGTCGGTCTCTCTCTGTCGCTCGAGAAGGGTTTGCCGTTGGGGAGCGGATTGGGATCTAGCGCGGCGAGCGCAGCCGCTGCGGCGATTGCTGTTAATGGATTGTTCGGCGGAAAATTAGGAGTCGAGGAATTGGTACTCGCGGGGCTGAAATCGGAAGAAAAGGTTTCTGGATACCATGCGGACAATGTCGCACCAGCGATCATGGGGGGTTTCATTCTGATCCGGAATTACGAACCCTTGGAACTGATCCGCCTGAAGTTCCCCGTCGAGAAGGAGCTGTTCTTCGTATTGGTGAGCCCGGAATTCGAAGCGCCGACCAAGAAAATGCGCGCTGCATTGCCGGCTGAAGTTGGGATGCCGCACCATGTATGGAACTGCAGCCAAGCCGGGGCGTTGGTGGCGGCGGTGCTGCAGGGCGACTTGGTGGGGCTAGGCAAGGCATTGTCATCGGACAAAATTGTGGAGCCAAGGCGATCCCCGCTGATTCCAGGGATGGATGGGGTCAAGAAGGCGGCGATTGCAGCCGGGGCATTCGGGTGCACGATAAGCGGGGCGGGGCCGACCGCGGTGGCAGTGATCGACGACGAGGAGAGGGGGAAGGAGATCGGTGAGAGGATGGTTATGGCGTTTCTGAAGGAGGGGAATTTGAAAGCCGTGGCATCAGTAAAGAGGCTCGATCGAGTTGGTGCAAGGCTTGTTGGCTCAACTCCT

mRNA sequence

ATGGCGATCTGCTTTCAGCCAACATTGAAGCCGGTGGCGGTTCCTCCAGTTTCTCTATCTAACCCTAAATCTGTCCTCTTCAGGTGCAATTTGTCTCTACCATCCAGGACCGCCGTCACTACGGTCGAACCTGAACCTGTATTCTCCTCTGTCAAAGCATTCGCACCTGCAACCGTCGCTAATTTAGGCCCTGGCTTCGATTTCCTCGGCTGCGCTGTCGATGGACTCGGCGATTTCGTCTCCCTCAGCGTTGATTCCAATGTTCATCCTGGTGAGGTCGCAATCACCAATATTTCTGGCAATAACACGAATAAGCTTAGTAAAAACCCTTTGTACAATTGCGCGGGCATTGCCGCTATCGAGGTCATGAAAATGCTAGGGATCCGATCTGTCGGTCTCTCTCTGTCGCTCGAGAAGGGTTTGCCGTTGGGGAGCGGATTGGGATCTAGCGCGGCGAGCGCAGCCGCTGCGGCGATTGCTGTTAATGGATTGTTCGGCGGAAAATTAGGAGTCGAGGAATTGGTACTCGCGGGGCTGAAATCGGAAGAAAAGGTTTCTGGATACCATGCGGACAATGTCGCACCAGCGATCATGGGGGGTTTCATTCTGATCCGGAATTACGAACCCTTGGAACTGATCCGCCTGAAGTTCCCCGTCGAGAAGGAGCTGTTCTTCGTATTGGTGAGCCCGGAATTCGAAGCGCCGACCAAGAAAATGCGCGCTGCATTGCCGGCTGAAGTTGGGATGCCGCACCATGTATGGAACTGCAGCCAAGCCGGGGCGTTGGTGGCGGCGGTGCTGCAGGGCGACTTGGTGGGGCTAGGCAAGGCATTGTCATCGGACAAAATTGTGGAGCCAAGGCGATCCCCGCTGATTCCAGGGATGGATGGGGTCAAGAAGGCGGCGATTGCAGCCGGGGCATTCGGGTGCACGATAAGCGGGGCGGGGCCGACCGCGGTGGCAGTGATCGACGACGAGGAGAGGGGGAAGGAGATCGGTGAGAGGATGGTTATGGCGTTTCTGAAGGAGGGGAATTTGAAAGCCGTGGCATCAGTAAAGAGGCTCGATCGAGTTGGTGCAAGGCTTGTTGGCTCAACTCCT

Coding sequence (CDS)

ATGGCGATCTGCTTTCAGCCAACATTGAAGCCGGTGGCGGTTCCTCCAGTTTCTCTATCTAACCCTAAATCTGTCCTCTTCAGGTGCAATTTGTCTCTACCATCCAGGACCGCCGTCACTACGGTCGAACCTGAACCTGTATTCTCCTCTGTCAAAGCATTCGCACCTGCAACCGTCGCTAATTTAGGCCCTGGCTTCGATTTCCTCGGCTGCGCTGTCGATGGACTCGGCGATTTCGTCTCCCTCAGCGTTGATTCCAATGTTCATCCTGGTGAGGTCGCAATCACCAATATTTCTGGCAATAACACGAATAAGCTTAGTAAAAACCCTTTGTACAATTGCGCGGGCATTGCCGCTATCGAGGTCATGAAAATGCTAGGGATCCGATCTGTCGGTCTCTCTCTGTCGCTCGAGAAGGGTTTGCCGTTGGGGAGCGGATTGGGATCTAGCGCGGCGAGCGCAGCCGCTGCGGCGATTGCTGTTAATGGATTGTTCGGCGGAAAATTAGGAGTCGAGGAATTGGTACTCGCGGGGCTGAAATCGGAAGAAAAGGTTTCTGGATACCATGCGGACAATGTCGCACCAGCGATCATGGGGGGTTTCATTCTGATCCGGAATTACGAACCCTTGGAACTGATCCGCCTGAAGTTCCCCGTCGAGAAGGAGCTGTTCTTCGTATTGGTGAGCCCGGAATTCGAAGCGCCGACCAAGAAAATGCGCGCTGCATTGCCGGCTGAAGTTGGGATGCCGCACCATGTATGGAACTGCAGCCAAGCCGGGGCGTTGGTGGCGGCGGTGCTGCAGGGCGACTTGGTGGGGCTAGGCAAGGCATTGTCATCGGACAAAATTGTGGAGCCAAGGCGATCCCCGCTGATTCCAGGGATGGATGGGGTCAAGAAGGCGGCGATTGCAGCCGGGGCATTCGGGTGCACGATAAGCGGGGCGGGGCCGACCGCGGTGGCAGTGATCGACGACGAGGAGAGGGGGAAGGAGATCGGTGAGAGGATGGTTATGGCGTTTCTGAAGGAGGGGAATTTGAAAGCCGTGGCATCAGTAAAGAGGCTCGATCGAGTTGGTGCAAGGCTTGTTGGCTCAACTCCT

Protein sequence

MAICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANLGPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARLVGSTP
Homology
BLAST of MS022246 vs. NCBI nr
Match: XP_022135832.1 (homoserine kinase [Momordica charantia])

HSP 1 Score: 697.6 bits (1799), Expect = 5.8e-197
Identity = 365/367 (99.46%), Postives = 366/367 (99.73%), Query Frame = 0

Query: 1   MAICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVA 60
           MAICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVA
Sbjct: 1   MAICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVA 60

Query: 61  NLGPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAI 120
           NLGPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAI
Sbjct: 61  NLGPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAI 120

Query: 121 EVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLK 180
           EVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLK
Sbjct: 121 EVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLK 180

Query: 181 SEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMR 240
           SEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMR
Sbjct: 181 SEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMR 240

Query: 241 AALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKK 300
           AALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSP+IPGMDGVKK
Sbjct: 241 AALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPMIPGMDGVKK 300

Query: 301 AAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGA 360
           AAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVA VKRLDRVGA
Sbjct: 301 AAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVALVKRLDRVGA 360

Query: 361 RLVGSTP 368
           RLVGSTP
Sbjct: 361 RLVGSTP 367

BLAST of MS022246 vs. NCBI nr
Match: XP_038888998.1 (homoserine kinase [Benincasa hispida])

HSP 1 Score: 664.1 bits (1712), Expect = 7.1e-187
Identity = 345/365 (94.52%), Postives = 359/365 (98.36%), Query Frame = 0

Query: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62
           I +QP LK +A+PPVSLSNPKSV+FRC+LSLPSRTAVT+VEPEPVFSSVKAFAPATVANL
Sbjct: 4   ISYQPPLKSLAIPPVSLSNPKSVIFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATVANL 63

Query: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122
           GPGFDFLGCAVDGLGD+VSLSVDSNVHPGEVAITNI+GNNTNKLSKNPLYNCAGIAAIEV
Sbjct: 64  GPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAAIEV 123

Query: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243

Query: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 302
           LP+EVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPSEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARL 362
           IAAGAFGCTISGAGPTAVAVID+EE+GKEIGERMVMAF+KEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVGARL 363

Query: 363 VGSTP 368
           +GSTP
Sbjct: 364 IGSTP 368

BLAST of MS022246 vs. NCBI nr
Match: XP_004153462.1 (homoserine kinase [Cucumis sativus] >KGN43352.1 hypothetical protein Csa_020446 [Cucumis sativus])

HSP 1 Score: 658.7 bits (1698), Expect = 3.0e-185
Identity = 342/365 (93.70%), Postives = 357/365 (97.81%), Query Frame = 0

Query: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62
           + +QP LK + +PPVSLSNPK VLFRC+LSLPSRTAVT+VEP+PVFSSVKAFAPATVANL
Sbjct: 4   LSYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRTAVTSVEPQPVFSSVKAFAPATVANL 63

Query: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122
           GPGFDFLGCAVDGLGD+VSLSVDSNVHPGEVAI++I+GNNTNKLSKNPLYNCAGIAAIEV
Sbjct: 64  GPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAAIEV 123

Query: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243

Query: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 302
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARL 362
           IAAGAFGCTISGAGPTAVAVID+EE+GKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 363

Query: 363 VGSTP 368
           +GSTP
Sbjct: 364 IGSTP 368

BLAST of MS022246 vs. NCBI nr
Match: KAA0059609.1 (homoserine kinase [Cucumis melo var. makuwa] >TYK08142.1 homoserine kinase [Cucumis melo var. makuwa])

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-184
Identity = 341/365 (93.42%), Postives = 356/365 (97.53%), Query Frame = 0

Query: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62
           I +QP LK + +PPVSLSNPK VLFRC+LSLPSR AVT+VEP+PVFSSVKAFAPATVANL
Sbjct: 4   ISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATVANL 63

Query: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122
           GPGFDFLGCAVDGLGD+VSLSVDSNVHPGEVAI++I+GNNTNKLSKNPLYNCAGIAAIEV
Sbjct: 64  GPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAAIEV 123

Query: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKMRAA 243

Query: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 302
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARL 362
           IAAGAFGCTISGAGPTAVAVID+EE+GKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKAAASVKRLDRVGARL 363

Query: 363 VGSTP 368
           +GSTP
Sbjct: 364 IGSTP 368

BLAST of MS022246 vs. NCBI nr
Match: XP_008451216.1 (PREDICTED: homoserine kinase [Cucumis melo])

HSP 1 Score: 656.0 bits (1691), Expect = 1.9e-184
Identity = 341/365 (93.42%), Postives = 356/365 (97.53%), Query Frame = 0

Query: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62
           I +QP LK + +PPVSLSNPK VLFRC+LSLPSR AVT+VEP+PVFSSVKAFAPATVANL
Sbjct: 4   ISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATVANL 63

Query: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122
           GPGFDFLGCAVDGLGD+VSLSVDSNVHPGEVAI++I+GNNTNKLSKNPLYNCAGIAAIEV
Sbjct: 64  GPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAAIEV 123

Query: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKMRAA 243

Query: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 302
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARL 362
           IAAGAFGCTISGAGPTAVAVID+EE+GKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEQGKEIGERMVMAFLKEGNLKAAASVKRLDRVGARL 363

Query: 363 VGSTP 368
           +GSTP
Sbjct: 364 IGSTP 368

BLAST of MS022246 vs. ExPASy Swiss-Prot
Match: Q8L7R2 (Homoserine kinase OS=Arabidopsis thaliana OX=3702 GN=HSK PE=1 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 6.3e-138
Identity = 269/369 (72.90%), Postives = 305/369 (82.66%), Query Frame = 0

Query: 2   AICFQPTLKPVA-VPPVSLSNP----KSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAP 61
           ++CFQ   KP++   P S  +P    K  +FRC  S+ +  A   VEPEPVF SVK FAP
Sbjct: 3   SLCFQSPSKPISYFQPKSNPSPPLFAKVSVFRCRASVQTLVA---VEPEPVFVSVKTFAP 62

Query: 62  ATVANLGPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAG 121
           ATVANLGPGFDFLGCAVDGLGD V+L VD +V  GEV+I+ I+G  T KLS NPL NCAG
Sbjct: 63  ATVANLGPGFDFLGCAVDGLGDHVTLRVDPSVRAGEVSISEITG-TTTKLSTNPLRNCAG 122

Query: 122 IAAIEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVL 181
           IAAI  MKMLGIRSVGLSL L KGLPLGSGLGSSAASAAAAA+AVN +FG KLG ++LVL
Sbjct: 123 IAAIATMKMLGIRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKLGSDQLVL 182

Query: 182 AGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPT 241
           AGL+SE KVSGYHADN+APAIMGGF+LIRNYEPL+L  L+FP +K+LFFVLVSP+FEAPT
Sbjct: 183 AGLESEAKVSGYHADNIAPAIMGGFVLIRNYEPLDLKPLRFPSDKDLFFVLVSPDFEAPT 242

Query: 242 KKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMD 301
           KKMRAALP E+ M HHVWN SQA ALVAAVL+GD V LGKALSSDKIVEP R+PLIPGM+
Sbjct: 243 KKMRAALPTEIPMVHHVWNSSQAAALVAAVLEGDAVMLGKALSSDKIVEPTRAPLIPGME 302

Query: 302 GVKKAAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLD 361
            VKKAA+ AGAFGCTISGAGPTAVAVID EE+G+ IGE+MV AF K G+LK+VASVK+LD
Sbjct: 303 AVKKAALEAGAFGCTISGAGPTAVAVIDSEEKGQVIGEKMVEAFWKVGHLKSVASVKKLD 362

Query: 362 RVGARLVGS 366
            VGARLV S
Sbjct: 363 NVGARLVNS 367

BLAST of MS022246 vs. ExPASy Swiss-Prot
Match: A4SGN0 (Homoserine kinase OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=290318 GN=thrB PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.5e-46
Identity = 135/322 (41.93%), Postives = 184/322 (57.14%), Query Frame = 0

Query: 50  SVKAFAPATVANLGPGFDFLGCAVDGLGDFVSLSVDSNVHPG-EVAITNISGNNTNKLSK 109
           SV+ FA ATV N+  GFD LG A+   GD V L+     H G  V I++ISG+   KL  
Sbjct: 3   SVRGFASATVGNVACGFDILGFAITEPGDEVILTRSERKHTGCPVTISSISGDG-GKLPL 62

Query: 110 NPLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAIAVNG 169
           +P  N +    ++ ++ +    G    G +SL L+K LPL SG+GSSAASAAAA  A N 
Sbjct: 63  DPRKNTSSFVVLKFLEHIRTHKGQDCTGHISLELKKHLPLSSGMGSSAASAAAALAAANE 122

Query: 170 LFGGKLGVEELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKE 229
           L G      ELV   ++ E    G  HADN APAI+G F+LIR+Y PL+LI +  P  ++
Sbjct: 123 LLGRPCTKMELVHFAIEGERVACGSAHADNAAPAILGNFVLIRSYTPLDLIVI--PPPEK 182

Query: 230 LFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDK 289
           LF  LV P  E  T   R+ LP ++ +          GALVA +L  D   +G++L  D 
Sbjct: 183 LFCSLVHPHTELRTSFARSVLPTDITLKDATRQWGNVGALVAGLLTSDYALVGRSL-VDV 242

Query: 290 IVEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFL- 349
           + EP+R+PLIPG   VK+AA+ AGA GC+I+G+GP+  A  D  E     GE M  AFL 
Sbjct: 243 VAEPKRAPLIPGFAEVKEAALNAGALGCSIAGSGPSIFAFSDSAETAHAAGEAMQKAFLA 302

Query: 350 KEGNLKAVASVKRLDRVGARLV 364
            + +LK+   V  + R GAR++
Sbjct: 303 SKKSLKSDMWVSPICREGARIL 320

BLAST of MS022246 vs. ExPASy Swiss-Prot
Match: B3QWX6 (Homoserine kinase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=517418 GN=thrB PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.4e-46
Identity = 132/320 (41.25%), Postives = 180/320 (56.25%), Query Frame = 0

Query: 51  VKAFAPATVANLGPGFDFLGCAVDGLGDFVSLS-VDSNVHPGEVAITNISGNNTNKLSKN 110
           V  FAPA+V N+  GFD LG A+   GD V++S  D    P +V I+ I G+    L  +
Sbjct: 4   VTGFAPASVGNVACGFDVLGFALTEPGDEVTVSFTDDENSPAQVIISKIIGDG-GALPTD 63

Query: 111 PLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAIAVNGL 170
           P  N A I  I+ ++ L    GI   G  S+ L+K LPL SG+GSSA+SAAAA IA N L
Sbjct: 64  PYKNTASIVVIKFLEFLKTHKGIEQSGTFSIELKKNLPLSSGMGSSASSAAAALIAANSL 123

Query: 171 FGGKLGVEELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKEL 230
            G      ELV   ++ E    G  HADN APA++G FIL R+Y+PL+LI +  P   EL
Sbjct: 124 LGSPCTKMELVPFVMEGERIACGSIHADNAAPAMLGNFILTRSYDPLDLIPISTP--PEL 183

Query: 231 FFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKI 290
           +  LV P  E PT   R+ L  E+ +   V      GALVA +L+ D   +G+AL  D +
Sbjct: 184 YCSLVHPHIEVPTSHARSILKKEIALSSAVRQWGNVGALVAGLLRCDYELIGRAL-EDVV 243

Query: 291 VEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKE 350
            EP R+PLIPG   VK AA+ AGA G +I+G+GP+  A  D   + + I + M   F K 
Sbjct: 244 AEPVRAPLIPGFYDVKHAALDAGALGGSIAGSGPSIFAFSDSLAKAETIAKAMQETFRKV 303

Query: 351 GNLKAVASVKRLDRVGARLV 364
            NL++      + R G+R++
Sbjct: 304 SNLESDIWFCPVSREGSRIL 319

BLAST of MS022246 vs. ExPASy Swiss-Prot
Match: Q8KAW7 (Homoserine kinase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=thrB PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.2e-45
Identity = 132/325 (40.62%), Postives = 183/325 (56.31%), Query Frame = 0

Query: 50  SVKAFAPATVANLGPGFDFLGCAVDGLGDFVSLSV-DSNVHPGEVAITNISGNNTNKLSK 109
           +V  FA ATV N+  GFD LG A+   GD V L++ D       V+IT+I G+    L  
Sbjct: 3   TVTGFASATVGNVACGFDVLGFAITEPGDEVVLALHDERRSDCPVSITSIVGDG-GALPL 62

Query: 110 NPLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAIAVNG 169
           +P  N +    ++ ++ +    GI   G + L L+K LPL SG+GSSAASAAAA IA N 
Sbjct: 63  DPKKNTSSFVVLKFLEYIRTTKGISFDGHIDLVLKKNLPLSSGMGSSAASAAAALIAANE 122

Query: 170 LFGGKLGVEELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKE 229
           LFG      ELV   ++ E    G  HADN APA++G FILIR+Y PL+LI +K P  K 
Sbjct: 123 LFGSPCTKMELVHFAIEGERVACGSAHADNAAPAMLGNFILIRSYNPLDLITIKPP--KN 182

Query: 230 LFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDK 289
           LF  LV P  E  T   R+ LP  + +          GAL+A +L  D   +G+AL  D 
Sbjct: 183 LFGTLVHPHTELKTSFARSVLPKSIPLSTATQQWGNVGALIAGLLMEDYDLIGRAL-VDV 242

Query: 290 IVEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFL- 349
           + EP+R+PLIPG + VK+AA+ AGA GC+I+G+GP+  A     +  + +G  M  AFL 
Sbjct: 243 VAEPKRAPLIPGFNEVKQAALDAGALGCSIAGSGPSVFAFSSSRQTAEAVGSAMQSAFLH 302

Query: 350 KEGNLKAVASVKRLDRVGARLVGST 367
               L++   V  +   GAR++ +T
Sbjct: 303 SRAALQSDMWVSPICSQGARIISTT 323

BLAST of MS022246 vs. ExPASy Swiss-Prot
Match: A1BJF6 (Homoserine kinase OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=thrB PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.8e-44
Identity = 130/322 (40.37%), Postives = 182/322 (56.52%), Query Frame = 0

Query: 50  SVKAFAPATVANLGPGFDFLGCAVDGLGDFVSLSV-DSNVHPGEVAITNISGNNTNKLSK 109
           +V+ FA ATV N+  GFD LG A+   GD V L++ +       V+IT ISG+    L  
Sbjct: 3   TVRGFASATVGNVACGFDVLGFAITEPGDEVILTLHEERKSECPVSITAISGDG-GALPL 62

Query: 110 NPLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAIAVNG 169
           +P  N +    ++ ++ +    GI   G + L L+K LPL SG+GSSAASAAAA  A N 
Sbjct: 63  DPKKNTSSFVVLKFLEYIRTSKGIDFKGHIDLELKKNLPLSSGMGSSAASAAAALAAANE 122

Query: 170 LFGGKLGVEELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKE 229
           L G      ELV   ++ E    G  HADN APAI+G F+LIR+Y PL+LI +  P  + 
Sbjct: 123 LLGQPCSKMELVHFAVEGERVACGSAHADNAAPAILGNFVLIRSYAPLDLITI--PSPEH 182

Query: 230 LFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDK 289
           LF  LV P  E  T   R+ LP  + +          GAL+A +L+ D   +G+AL  D 
Sbjct: 183 LFCTLVHPHTELKTSFARSVLPRSIPLKTATQQWGNVGALIAGLLKSDYDLIGRAL-VDV 242

Query: 290 IVEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAF-L 349
           + EP+R+PLIPG   VK AAI  GA GC+I+G+GP+  A    +E  + +G+ M  AF L
Sbjct: 243 VAEPKRAPLIPGFLDVKHAAIDCGALGCSIAGSGPSVFAFSSSKETAERVGQAMREAFLL 302

Query: 350 KEGNLKAVASVKRLDRVGARLV 364
            E NLK+   V  + + GA+++
Sbjct: 303 PETNLKSDMWVSPICKEGAKVL 320

BLAST of MS022246 vs. ExPASy TrEMBL
Match: A0A6J1C3W0 (Homoserine kinase OS=Momordica charantia OX=3673 GN=LOC111007689 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 2.8e-197
Identity = 365/367 (99.46%), Postives = 366/367 (99.73%), Query Frame = 0

Query: 1   MAICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVA 60
           MAICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVA
Sbjct: 1   MAICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVA 60

Query: 61  NLGPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAI 120
           NLGPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAI
Sbjct: 61  NLGPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAI 120

Query: 121 EVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLK 180
           EVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLK
Sbjct: 121 EVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLK 180

Query: 181 SEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMR 240
           SEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMR
Sbjct: 181 SEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMR 240

Query: 241 AALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKK 300
           AALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSP+IPGMDGVKK
Sbjct: 241 AALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPMIPGMDGVKK 300

Query: 301 AAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGA 360
           AAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVA VKRLDRVGA
Sbjct: 301 AAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVALVKRLDRVGA 360

Query: 361 RLVGSTP 368
           RLVGSTP
Sbjct: 361 RLVGSTP 367

BLAST of MS022246 vs. ExPASy TrEMBL
Match: A0A0A0K4Y4 (Homoserine kinase OS=Cucumis sativus OX=3659 GN=Csa_7G025730 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.4e-185
Identity = 342/365 (93.70%), Postives = 357/365 (97.81%), Query Frame = 0

Query: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62
           + +QP LK + +PPVSLSNPK VLFRC+LSLPSRTAVT+VEP+PVFSSVKAFAPATVANL
Sbjct: 4   LSYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRTAVTSVEPQPVFSSVKAFAPATVANL 63

Query: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122
           GPGFDFLGCAVDGLGD+VSLSVDSNVHPGEVAI++I+GNNTNKLSKNPLYNCAGIAAIEV
Sbjct: 64  GPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAAIEV 123

Query: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243

Query: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 302
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARL 362
           IAAGAFGCTISGAGPTAVAVID+EE+GKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 363

Query: 363 VGSTP 368
           +GSTP
Sbjct: 364 IGSTP 368

BLAST of MS022246 vs. ExPASy TrEMBL
Match: A0A5A7V1E5 (Homoserine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00200 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 7.2e-185
Identity = 341/365 (93.42%), Postives = 356/365 (97.53%), Query Frame = 0

Query: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62
           I +QP LK + +PPVSLSNPK VLFRC+LSLPSR AVT+VEP+PVFSSVKAFAPATVANL
Sbjct: 4   ISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATVANL 63

Query: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122
           GPGFDFLGCAVDGLGD+VSLSVDSNVHPGEVAI++I+GNNTNKLSKNPLYNCAGIAAIEV
Sbjct: 64  GPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAAIEV 123

Query: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKMRAA 243

Query: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 302
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARL 362
           IAAGAFGCTISGAGPTAVAVID+EE+GKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKAAASVKRLDRVGARL 363

Query: 363 VGSTP 368
           +GSTP
Sbjct: 364 IGSTP 368

BLAST of MS022246 vs. ExPASy TrEMBL
Match: A0A1S3BR18 (Homoserine kinase OS=Cucumis melo OX=3656 GN=LOC103492575 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 9.4e-185
Identity = 341/365 (93.42%), Postives = 356/365 (97.53%), Query Frame = 0

Query: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62
           I +QP LK + +PPVSLSNPK VLFRC+LSLPSR AVT+VEP+PVFSSVKAFAPATVANL
Sbjct: 4   ISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATVANL 63

Query: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122
           GPGFDFLGCAVDGLGD+VSLSVDSNVHPGEVAI++I+GNNTNKLSKNPLYNCAGIAAIEV
Sbjct: 64  GPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAAIEV 123

Query: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKMRAA 243

Query: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 302
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARL 362
           IAAGAFGCTISGAGPTAVAVID+EE+GKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEQGKEIGERMVMAFLKEGNLKAAASVKRLDRVGARL 363

Query: 363 VGSTP 368
           +GSTP
Sbjct: 364 IGSTP 368

BLAST of MS022246 vs. ExPASy TrEMBL
Match: A0A6J1GJP3 (Homoserine kinase OS=Cucurbita moschata OX=3662 GN=LOC111454929 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 5.0e-178
Identity = 333/365 (91.23%), Postives = 345/365 (94.52%), Query Frame = 0

Query: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62
           I FQP LK  AVP V L NPKSV   C+LSLPSRT VT VEP PVF+SVKAFAPATVANL
Sbjct: 4   ISFQPPLKSFAVPQVPLYNPKSVAVCCSLSLPSRTVVTAVEPAPVFASVKAFAPATVANL 63

Query: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122
           GPGFDFLGCAVDGLGDFVSLSVDSNV PGEVAITNI+GN+ NKLSKNPLYNCAGIAAIEV
Sbjct: 64  GPGFDFLGCAVDGLGDFVSLSVDSNVRPGEVAITNITGNDPNKLSKNPLYNCAGIAAIEV 123

Query: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVE+LVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEDLVLAGLKSE 183

Query: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243

Query: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 302
           LPAEVGMPHHVWNCSQAGALVAAVLQGD++GLGKALSSDKIVEP R PLIPGM GVKKAA
Sbjct: 244 LPAEVGMPHHVWNCSQAGALVAAVLQGDVIGLGKALSSDKIVEPTRVPLIPGMGGVKKAA 303

Query: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARL 362
           IAAGAFGCTISGAGPTAVAV D++ERGKEIGE MVMAF++EGNLKAVASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVTDNKERGKEIGESMVMAFMREGNLKAVASVKRLDRVGARL 363

Query: 363 VGSTP 368
           +GSTP
Sbjct: 364 IGSTP 368

BLAST of MS022246 vs. TAIR 10
Match: AT2G17265.1 (homoserine kinase )

HSP 1 Score: 491.9 bits (1265), Expect = 4.5e-139
Identity = 269/369 (72.90%), Postives = 305/369 (82.66%), Query Frame = 0

Query: 2   AICFQPTLKPVA-VPPVSLSNP----KSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAP 61
           ++CFQ   KP++   P S  +P    K  +FRC  S+ +  A   VEPEPVF SVK FAP
Sbjct: 3   SLCFQSPSKPISYFQPKSNPSPPLFAKVSVFRCRASVQTLVA---VEPEPVFVSVKTFAP 62

Query: 62  ATVANLGPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAG 121
           ATVANLGPGFDFLGCAVDGLGD V+L VD +V  GEV+I+ I+G  T KLS NPL NCAG
Sbjct: 63  ATVANLGPGFDFLGCAVDGLGDHVTLRVDPSVRAGEVSISEITG-TTTKLSTNPLRNCAG 122

Query: 122 IAAIEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVL 181
           IAAI  MKMLGIRSVGLSL L KGLPLGSGLGSSAASAAAAA+AVN +FG KLG ++LVL
Sbjct: 123 IAAIATMKMLGIRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKLGSDQLVL 182

Query: 182 AGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPT 241
           AGL+SE KVSGYHADN+APAIMGGF+LIRNYEPL+L  L+FP +K+LFFVLVSP+FEAPT
Sbjct: 183 AGLESEAKVSGYHADNIAPAIMGGFVLIRNYEPLDLKPLRFPSDKDLFFVLVSPDFEAPT 242

Query: 242 KKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPLIPGMD 301
           KKMRAALP E+ M HHVWN SQA ALVAAVL+GD V LGKALSSDKIVEP R+PLIPGM+
Sbjct: 243 KKMRAALPTEIPMVHHVWNSSQAAALVAAVLEGDAVMLGKALSSDKIVEPTRAPLIPGME 302

Query: 302 GVKKAAIAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLD 361
            VKKAA+ AGAFGCTISGAGPTAVAVID EE+G+ IGE+MV AF K G+LK+VASVK+LD
Sbjct: 303 AVKKAALEAGAFGCTISGAGPTAVAVIDSEEKGQVIGEKMVEAFWKVGHLKSVASVKKLD 362

Query: 362 RVGARLVGS 366
            VGARLV S
Sbjct: 363 NVGARLVNS 367

BLAST of MS022246 vs. TAIR 10
Match: AT4G35295.1 (homoserine kinase, putative / HSK, putative )

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-12
Identity = 41/61 (67.21%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 305 AGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVASVKRLDRVGARLVG 364
           AGAFG TI     TAVAVID  E+G EIGE+MV  FLK GNLK+ ASVK+LD+VGARL+ 
Sbjct: 55  AGAFGRTI-----TAVAVIDTAEKGYEIGEKMVQVFLKIGNLKSTASVKKLDKVGARLIE 110

Query: 365 S 366
           S
Sbjct: 115 S 110

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135832.15.8e-19799.46homoserine kinase [Momordica charantia][more]
XP_038888998.17.1e-18794.52homoserine kinase [Benincasa hispida][more]
XP_004153462.13.0e-18593.70homoserine kinase [Cucumis sativus] >KGN43352.1 hypothetical protein Csa_020446 ... [more]
KAA0059609.11.5e-18493.42homoserine kinase [Cucumis melo var. makuwa] >TYK08142.1 homoserine kinase [Cucu... [more]
XP_008451216.11.9e-18493.42PREDICTED: homoserine kinase [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q8L7R26.3e-13872.90Homoserine kinase OS=Arabidopsis thaliana OX=3702 GN=HSK PE=1 SV=1[more]
A4SGN01.5e-4641.93Homoserine kinase OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=29031... [more]
B3QWX67.4e-4641.25Homoserine kinase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=51... [more]
Q8KAW72.2e-4540.62Homoserine kinase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC... [more]
A1BJF61.8e-4440.37Homoserine kinase OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=t... [more]
Match NameE-valueIdentityDescription
A0A6J1C3W02.8e-19799.46Homoserine kinase OS=Momordica charantia OX=3673 GN=LOC111007689 PE=3 SV=1[more]
A0A0A0K4Y41.4e-18593.70Homoserine kinase OS=Cucumis sativus OX=3659 GN=Csa_7G025730 PE=3 SV=1[more]
A0A5A7V1E57.2e-18593.42Homoserine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00... [more]
A0A1S3BR189.4e-18593.42Homoserine kinase OS=Cucumis melo OX=3656 GN=LOC103492575 PE=3 SV=1[more]
A0A6J1GJP35.0e-17891.23Homoserine kinase OS=Cucurbita moschata OX=3662 GN=LOC111454929 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G17265.14.5e-13972.90homoserine kinase [more]
AT4G35295.11.1e-1267.21homoserine kinase, putative / HSK, putative [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00958HOMSERKINASEcoord: 187..200
score: 52.78
coord: 227..244
score: 38.89
coord: 307..322
score: 47.92
coord: 58..73
score: 61.11
coord: 148..163
score: 45.83
NoneNo IPR availablePANTHERPTHR20861:SF8BNAA09G09000D PROTEINcoord: 5..365
NoneNo IPR availablePANTHERPTHR20861HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASEcoord: 5..365
IPR013750GHMP kinase, C-terminal domainPFAMPF08544GHMP_kinases_Ccoord: 263..336
e-value: 2.0E-10
score: 40.9
IPR000870Homoserine kinasePIRSFPIRSF000676Homoser_kincoord: 47..363
e-value: 1.3E-93
score: 311.2
IPR000870Homoserine kinaseTIGRFAMTIGR00191TIGR00191coord: 53..361
e-value: 3.9E-64
score: 214.3
IPR000870Homoserine kinaseHAMAPMF_00384Homoser_kinasecoord: 51..363
score: 27.189369
IPR006204GHMP kinase N-terminal domainPFAMPF00288GHMP_kinases_Ncoord: 135..200
e-value: 3.4E-10
score: 40.0
IPR036554GHMP kinase, C-terminal domain superfamilyGENE3D3.30.70.890coord: 221..351
e-value: 6.1E-37
score: 128.4
IPR036554GHMP kinase, C-terminal domain superfamilySUPERFAMILY55060GHMP Kinase, C-terminal domaincoord: 222..336
IPR014721Ribosomal protein S5 domain 2-type fold, subgroupGENE3D3.30.230.10coord: 45..220
e-value: 1.1E-45
score: 157.3
IPR006203GHMP kinase, ATP-binding, conserved sitePROSITEPS00627GHMP_KINASES_ATPcoord: 141..152
IPR020568Ribosomal protein S5 domain 2-type foldSUPERFAMILY54211Ribosomal protein S5 domain 2-likecoord: 50..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022246.1MS022246.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006566 threonine metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0004413 homoserine kinase activity